Feeding bottle feeding frequency monitoring device and lumbar intervertebral disc degeneration experiment system

Abstract

The utility model provides a feeding bottle feeding frequency monitoring device and a lumbar intervertebral disc degeneration experiment system. The feeding bottle feeding frequency monitoring device comprises a frame top plate which is provided with a through hole matched with the appearance of a bottle opening of a feeding bottle; the pair of frame side plates are respectively connected and oppositely arranged at two opposite ends of the frame top plate, and form a fixed frame with the frame top plate; the pair of clamping mechanisms are respectively arranged on the pair of frame side plates and are used for fixedly clamping the fixed frame at the bottle opening which is arranged in the through hole in a penetrating manner; the photoelectric sensor is arranged at the tail end, away from the frame top plate, of the frame side plate and used for detecting the frequency of the experimental animal entering the inner side of the fixed frame. The feeding bottle feeding frequency monitoring device can continuously monitor the feeding condition of an experimental mouse stretching and standing below the bottle opening for a long time, and the lumbago degree of the experimental mouse is effectively quantified and evaluated. The feeding bottle feeding frequency monitoring device is simple in structure, fast to assemble and low in manufacturing cost, an experimenter only needs to collect data and does not need to carry out other operations on an experimental mouse, and the burden of manpower and material resources is relieved.

Description

TECHNICAL FIELD

[0001] The utility model relates to lumbar intervertebral disc degeneration experimental device technical field, especially relates to a feeding bottle eating frequency monitoring devices and the lumbar intervertebral disc degeneration experimental system of application thereof. BACKGROUND

[0002] Low back pain is a common disease affecting the quality of life of people all over the world, and the incidence has gradually increased in recent years, causing a huge medical burden to the world. The main cause of low back pain is lumbar intervertebral disc degeneration. The current main method for studying intervertebral disc degeneration is to construct an animal intervertebral disc degeneration model to study the disease progression and degeneration mechanism of intervertebral disc degeneration.

[0003] For the evaluation of animal model low back pain, subjective questionnaire evaluation cannot be performed like humans, so the current main objective evaluation is pain assessment. The existing methods for evaluating rodent pain mainly include various mechanical, anatomical or chemical evaluation methods. Such as mouse rotarod test, dynamic weight bearing (DWB) system, spontaneous behavior evaluation, experimental animal behavior observation registration and analysis system (LABORAS), mechanical sensitivity-von Frey test, thermal sensitivity-Hargreaves test, etc. The above methods have certain defects, such as the pain standard of animals cannot be quantified, it is difficult to continuously monitor and evaluate for a long time, and a large amount of manpower and material resources are required, resulting in high monitoring cost, etc. UTILITY MODEL CONTENT

[0004] The utility model provides a kind of feeding bottle eating frequency monitoring devices and the lumbar intervertebral disc degeneration experimental system of application thereof, to solve the technical problems that the existing animal model low back pain evaluation standard cannot be quantified, it is difficult to monitor and evaluate for a long time.

[0005] To solve the above problems, the technical scheme adopted by the utility model is:

[0006] The utility model provides a kind of feeding bottle eating frequency monitoring devices, comprising:

[0007] Frame top plate is provided with the perforation matched with the bottle mouth shape of feeding bottle;

[0008] A pair of frame side plates are respectively connected to the opposite ends of frame top plate, and a pair of frame side plates are oppositely arranged, and a pair of frame side plates and frame top plate form a fixed frame;

[0009] A pair of clamping mechanisms are respectively arranged on a pair of frame side plates, for fixing and clamping the bottle mouth in the perforation;

[0010] Photoelectric sensor is arranged at the end of frame side plate away from frame top plate, for detecting the frequency of experimental animal entering the inside of fixed frame.

[0011] Preferably, the bottle mouth is in the shape of a circular tube, and the perforation is a circular hole matching the shape of the bottle mouth.

[0012] Preferably, the clamping mechanism comprises:

[0013] A fixing nut is fixedly arranged on the side plate of the frame;

[0014] An adjusting screw is movably arranged in the fixing nut, and an end of the adjusting screw extending into the inside of the fixing frame is provided with a clamping piece for clamping the bottle mouth in cooperation with the clamping piece of the other adjusting screw when the adjusting screw moves close to the bottle mouth.

[0015] Preferably, the clamping piece is an arc-shaped clamping piece matching the shape of the bottle mouth.

[0016] Preferably, the photoelectric sensor comprises a laser emitting device and a laser receiving device arranged at the distal ends of the pair of side plates away from the top plate of the frame.

[0017] Preferably, the photoelectric sensor is an NPN type normally open three-wire laser sensor.

[0018] The utility model also provides a lumbar intervertebral disc degeneration experiment system, including current recorder, switching power supply, still include above-mentioned feeding bottle eating frequency monitoring devices, photoelectric sensor is connected to switching power supply, photoelectric sensor still includes contact detection circuit connected to current recorder, is used for when photoelectric sensor judges experimental animal to enter the inside of fixed frame to current recorder output trigger signal.

[0019] Further, the lumbar intervertebral disc degeneration experiment system further comprises:

[0020] A breeding cage;

[0021] A water feeding bottle filled with clean water is installed on the inside of the breeding cage.

[0022] A sugar water feeding bottle filled with sugar water is installed on the inside of the breeding cage higher than the water feeding bottle.

[0023] Compared with the prior art, the utility model has the following beneficial effects:

[0024] The feeding bottle eating frequency monitoring device provided by the utility model monitors whether there is an object blocking the bottle mouth below the bottle mouth of the feeding bottle through the photoelectric sensor, and further can long-term continuously monitor the condition that the experimental mouse stands and eats below the bottle mouth, and the current recorder and the central control device connected with the photoelectric sensor are used to count the eating frequency of the experimental mouse at the bottle mouth, so that the degree of lumbar pain of the experimental mouse is effectively quantitatively evaluated. Meanwhile, the feeding bottle eating frequency monitoring device provided by the utility model has the advantages of simple structure, fast assembly, low manufacturing cost, and the experimental personnel only need to collect data without performing other operations on the experimental mouse, so that the burden of manpower and material resources is greatly reduced. Attached Figure Description

[0025] To more clearly illustrate the technical solution proposed by this utility model, the present utility model will be described in detail below with reference to the embodiments and accompanying drawings. It should be understood that the embodiments and accompanying drawings described in the following detailed description are merely some embodiments of this utility model, and those skilled in the art can make changes to these drawings under the concept of this utility model.

[0026] Figure 1 A perspective view of an embodiment of the feeding bottle feeding frequency monitoring device provided by this utility model;

[0027] Figure 2 A front view schematic diagram of an embodiment of the feeding bottle feeding frequency monitoring device provided by this utility model;

[0028] Figure 3 A side view schematic diagram of an embodiment of the feeding bottle feeding frequency monitoring device provided by this utility model;

[0029] Figure 4 A top view schematic diagram of an embodiment of the feeding bottle feeding frequency monitoring device provided by this utility model;

[0030] Figure 5 This is a schematic diagram of an embodiment of the experimental system for lumbar intervertebral disc degeneration provided by this utility model.

[0031] The main markings in the attached figures are as follows:

[0032] 1. Frame top plate; 11. Perforation; 2. Frame side plate; 3. Clamping mechanism; 31. Fixing nut; 32. Adjusting screw; 33. Clamping component; 4. Photoelectric sensor; 41. Laser emitting device; 42. Laser receiving device; 5. Feeding bottle; 51. Bottle mouth; 6. Breeding cage; 7. Contact detection circuit; 8. Water bottle; 9. Laboratory mouse. Detailed Implementation

[0033] To make the technical problem to be solved, the technical solution and the beneficial effects of this utility model clearer, the following description is provided in conjunction with the appendix. Figures 1-5 The present invention will be further described in detail with reference to the embodiments.

[0034] Please refer to the following: Figures 1-5 The feeding bottle feeding frequency monitoring device provided by this utility model includes:

[0035] The frame top plate 1 is provided with a through hole 11 matched with the shape of the bottle mouth 51 of the feeding bottle 5; a pair of frame side plates 2 are respectively connected to the opposite ends of the frame top plate 1, and the pair of frame side plates 2 are oppositely arranged, and the pair of frame side plates 2 and the frame top plate 1 form a fixed frame; a pair of clamping mechanisms 3 are respectively arranged on the pair of frame side plates 2, and are used for clamping and fixing the fixed frame on the bottle mouth 51 arranged in the through hole 11; and the photoelectric sensor 4 is arranged at the end of the frame side plate 2 away from the frame top plate 1, and is used for triggering the detection of the frequency of the experimental animal entering the inside space of the fixed frame by shielding or reflecting the light signal of the object (i.e. the head or body part of the experimental animal).

[0036] Please refer to Figures 1-4 In the embodiment, the bottle mouth 51 of the feeding bottle 5 is in an elongated circular tube shape, and the above-mentioned through hole 11 of the frame top plate 1 is a circular hole matched with the shape of the bottle mouth 51, which is used for allowing the bottle mouth 51 of the feeding bottle 5 in the elongated circular tube shape to pass through, so that the bottle mouth 51 of the inverted feeding bottle 5 extends into the inside space of the fixed frame from above the frame top plate 1 through the through hole 11.

[0037] In other embodiments, the bottle mouth 51 of the feeding bottle 5 can also be in a square tube or rectangular tube shape, and the above-mentioned through hole 11 can also be a square hole or a rectangular hole matched with the shape of the bottle mouth 51.

[0038] Please refer to Figures 1-4 In the embodiment, the clamping mechanism 3 includes:

[0039] The fixed nut 31 is fixedly arranged in the frame side plate 2; the adjusting screw 32 is movably arranged in the fixed nut 31, and one end of the adjusting screw 32 extending into the inside of the fixed frame is provided with a clamping piece 33, which is used for cooperating with the clamping piece 33 of the adjusting screw 32 arranged on the other frame side plate 2 (of the fixed nut 31) when the adjusting screw 32 moves close to the bottle mouth 51, and the corresponding clamping pieces 33 of the pair of adjusting screws 32 move close to the bottle mouth 51 until both of them abut against the bottle mouth 51, so that the fixed frame is clamped and fixed on the bottle mouth 51 arranged in the through hole 11 by the pair of clamping pieces 33, to perform subsequent monitoring.

[0040] When it is necessary to disassemble the feeding bottle 5 from the fixed frame, the pair of clamping pieces 33 can be loosened by moving the pair of adjusting screws 32 away from each other, and then the bottle mouth 51 is withdrawn from the through hole 11 of the frame top plate 1, so that the disassembly of the feeding bottle 5 is finally completed.

[0041] Please refer to Figures 1-4 As a preferred embodiment of the embodiment, the clamping piece 33 is an arc-shaped clamping piece matched with the shape of the bottle mouth 51.

[0042] In other embodiments of the present application, the clamping member 33 can also be in the form of a clamping strip or clamping block with an arc-shaped clamping groove matching the shape of the bottle mouth 51.

[0043] In other embodiments of the present application, the clamping mechanism 3 can also be driven by a cylinder mechanism or other reset mechanism to drive the clamping member 33 to open and close to clamp or release the bottle mouth 51.

[0044] In the present embodiment, the photoelectric sensor 4 can be a pair of photoelectric sensors 4, a reflective plate type photoelectric switch or a diffuse reflection type photoelectric switch.

[0045] Please refer to Figures 1-5 , as a preferred embodiment of the present application, the photoelectric sensor 4 is a pair of photoelectric sensors 4, including a laser emitting device 41 and a laser receiving device 42, respectively arranged at the ends of a pair of frame side plates 2 away from the frame top plate 1, when the object (i.e. the experimental mouse) enters between the laser emitting device 41 and the laser receiving device 42, the laser receiving device 42 blocks the light signal emitted by the laser emitting device 41, at this time the sensor outputs a trigger signal.

[0046] As a more preferred embodiment of the present application, the photoelectric sensor 4 is an NPN type normally open three-wire laser sensor.

[0047] Please refer to Figure 5 , the utility model also provides a lumbar intervertebral disc degeneration experiment system, including the central control device (not shown in the drawing), current recorder (not shown in the drawing), switching power supply (not shown in the drawing), still including the above-mentioned feeding bottle 5 eating frequency monitoring device, photoelectric sensor 4 is connected to switching power supply, photoelectric sensor 4 still includes the contact detection circuit 7 connected to current recorder, for when photoelectric sensor 4 judges experimental animal to enter inside fixed frame to the current recorder output trigger signal, by current recorder record and the data is conducted to the central control device of lumbar intervertebral disc degeneration experiment system and is saved, for its subsequent data analysis.

[0048] Please refer to Figure 5 , in the present embodiment, the lumbar intervertebral disc degeneration experiment system further includes:

[0049] The breeding cage 6 is provided with a door body for experimental animals to enter and exit, for breeding experimental animals; the water bottle 8 is filled with clean water and is installed inside the breeding cage 6.

[0050] The above-mentioned feeding bottle 5 is a sugar water feeding bottle 5 filled with sugar water (such as sucrose or glucose solution) and is installed inside the breeding cage 6 at a position higher than the water bottle 8.

[0051] Please refer to Figure 5, as the preferred embodiment of the present embodiment, the cage 6 is strip-shaped and horizontally placed, the top surface of the cage 6 is connected with the corresponding side surface to form a transition inclined surface with an inclination, and the water feeding bottle 8 is vertically arranged on the transition inclined surface (i.e. obliquely downward), so that the water inlet of the water feeding bottle 8 extends into the inner space of the fixed frame.

[0052] The feeding bottle 5 is arranged vertically on the horizontal top surface of the cage 6 (i.e. the feeding bottle 5 is vertically arranged), so that the elongated tubular bottle mouth 51 of the feeding bottle 5 extends into the inner space of the fixed frame, and the height of the bottle mouth 51 of the feeding bottle 5 is higher than the height of the water inlet of the feeding bottle 5.

[0053] Please refer to Figure 5 In the present embodiment, the experimental animal is preferably an experimental mouse 9 (experimental mouse).

[0054] Please refer to Figure 5 The working principle of the lumbar intervertebral disc degeneration experiment system provided by the utility model is as follows:

[0055] First, the perforation 11 on the frame top plate 1 is passed through the elongated tubular bottle mouth 51 of the inverted feeding bottle 5 from bottom to top (downward), and the bottle mouth 51 extending into the inner space of the fixed frame is clamped by adjusting a pair of adjusting screws 32 to drive a pair of clamping members 33 to move towards each other until the bottle mouth 51 is clamped, so as to fix and clamp the feeding frequency monitoring device of the feeding bottle 5 at the bottle mouth 51 of the feeding bottle 5.

[0056] Then, the laser emitting device 41 and the laser receiving device 42 of the NPN normally open three-wire laser sensor are respectively fixed on the ends of a pair of frame side plates 2 away from the frame top plate 1 and are respectively connected to the switching power supply, and the current recorder is connected to the contact detection circuit 7, so that the installation operation of the feeding frequency monitoring device of the feeding bottle 5 is completed.

[0057] When the experiment is performed, the experimental mouse 9 for the lumbar intervertebral disc degeneration model is placed in the cage 6 and the cage 6 is closed, and the experimental mouse 9 will reduce the frequency of drinking water due to back pain, especially the frequency of sucking sugar water from the bottle mouth 51 of the higher feeding bottle 5 by stretching the waist, and the behavior will be sensed by the feeding frequency monitoring device of the feeding bottle 5, and when the head or other body parts of the experimental mouse extend between the laser emitting device 41 and the laser receiving device 42, the current recorder will record and transmit the data to the central control device for storage, so that the central control device can analyze and count the data subsequently.

[0058] Please refer to Figure 5 The utility model also provides a lumbar intervertebral disc degeneration experiment method applied to the above lumbar intervertebral disc degeneration experiment system, which comprises the following steps:

[0059] The solid feed and clean water are not restricted in the feeding cage 6, and the feeding bottle 5 containing sugar water is fixedly installed at a higher position in the feeding cage 6 (to ensure that the bottle mouth 51 of the feeding bottle 5 can be touched by the experimental mouse 9 standing with the waist stretched), and the water bottle 8 containing clean water is fixedly installed at a lower position in the feeding cage 6.

[0060] According to the sweet behavior of the experimental mouse 9, the experimental mouse 9 will prefer to drink sugar water. After the experimental mouse 9 is adaptively fed in the feeding cage 6 for 3-5 days, the feeding bottle 5 food intake frequency monitoring device is installed on the feeding bottle 5 containing sugar water according to the above installation mode, and the positions of the solid feed and the water bottle 8 providing clean water in the feeding cage 6 remain unchanged. The experimental mouse 9 is placed in the feeding cage 6. Since the experimental mouse 9 will feel waist pain when stretching the waist due to lumbar disc degeneration, it will naturally reduce the drinking of sugar water in the feeding bottle 5 at a higher position. This phenomenon will be perceived and recorded by the feeding bottle 5 food intake frequency monitoring device.

[0061] The specific experimental procedure of the lumbar disc degeneration experiment is as follows:

[0062] The experiment includes four stages of adaptation period, baseline test, experimental test and data analysis, and takes about eight days.

[0063] S1. Adaptation period:

[0064] S11. Sucrose solution adaptation stage (1st day to 3rd day): from 5 pm on the 1st day, the feeding bottle 5 containing 1% sucrose solution and the water bottle 8 containing ordinary clean water are placed in the corresponding positions of the control feeding cage 6 for 48 hours, and the installation heights of the feeding bottle 5 and the water bottle 8 in the control feeding cage 6 are the same, until 5 pm on the 3rd day. In this stage, in order to avoid the experimental mouse 9 forming a position preference, the positions of the feeding bottle 5 and the water bottle 8 should be changed every day.

[0065] S12. Experimental equipment adaptation stage (3rd day to 4th day): at 5 pm on the 3rd day, another experimental feeding cage 6 is cleaned with 70% ethanol or odorless disinfectant water and dried. Fresh 1% sucrose solution and ordinary clean water are placed in the feeding bottle 5 and the water bottle 8 of the experimental feeding cage 6, respectively, and an appropriate amount of solid feed is placed in the food trough of the experimental feeding cage 6. At the same time, the installation heights of the feeding bottle 5 and the water bottle 8 in the experimental feeding cage 6 remain the same.

[0066] The experimental mouse 9 adapted to the sucrose solution is transferred from the control feeding cage 6 to the prepared experimental feeding cage 6. At this time, the experimental mouse 9 can freely contact two different solutions (1% sucrose solution and ordinary clean water) and solid feed.

[0067] On the fourth day, the feeding bottle 5 with sugar water is installed in the experimental cage 6 in the same way as described above, and the experimental mouse 9 needs to stretch its neck to reach the bottle mouth 51, and the water bottle 8 with ordinary water is installed in the experimental cage 6, which is easy for the experimental mouse 9 to reach and is lower than the feeding bottle 5. This step is continued for one day until 5:00 pm on the fourth day, and then the experimental mouse 9 is returned to the control cage 6 and provided with ordinary water and solid feed.

[0068] S2. Baseline determination:

[0069] The experimental cage 6 is prepared in advance, and fresh 1% sucrose solution and ordinary water are placed in the feeding bottle 5 and the water bottle 8 of the experimental cage 6, respectively, and an appropriate amount of solid feed is placed in the feeding trough of the experimental cage 6.

[0070] S3. Experimental test:

[0071] First test: At 9:00 pm on the fourth day, the experimental mouse 9 is transferred from the control cage 6 to the experimental cage 6, and the intake amount, intake frequency, and intake time of the experimental mouse 9 for the two solutions are recorded from 9:00 pm on the same day to 9:00 am on the fifth day, a total of 12 hours. After the first test, the experimental mouse 9 is returned to the control cage 6 and provided with ordinary water and solid feed. At the same time, the experimental cage 6 is cleaned for subsequent use.

[0072] Second test: At 9:00 pm on the fifth day, the experimental mouse 9 is again transferred from the control cage 6 to the experimental cage 6 (note that the positions of the feeding bottle 5 and the water bottle 8 need to be changed to avoid the experimental mouse 9 forming a position preference), and the intake amount, intake frequency, and intake time of the experimental mouse 9 for the two solutions are recorded from 9:00 pm on the same day to 9:00 am on the sixth day, a total of 12 hours. After the second test, the experimental mouse 9 is returned to the control cage 6 and provided with ordinary water and solid feed.

[0073] S4. Record the experimental data obtained in the two tests, and complete the subsequent comparison and analysis of the experimental data.

[0074] In another experimental scheme, the experimental mouse 9 can also be continuously raised in the experimental cage 6 and not returned to the ordinary control cage 6 during the experiment, so that the change process of the experimental mouse 9's waist pain data can be continuously measured.

[0075] The above is only a preferred embodiment of the present application, and is not intended to limit the present application. Those skilled in the art should understand that any modification, equivalent replacement, and improvement made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A feeding bottle feeding frequency monitoring device, characterised in that, include: The top plate of the frame (1) is provided with perforations (11) that match the shape of the bottle mouth (51) of the feeding bottle (5); A pair of frame side plates (2) are respectively connected to the opposite ends of the frame top plate (1), and the pair of frame side plates (2) are arranged opposite to each other, and the pair of frame side plates (2) and the frame top plate (1) form a fixed frame. A pair of clamping mechanisms (3) are respectively provided on a pair of frame side plates (2) for fixing the fixed frame to the bottle mouth (51) passing through the perforation (11); A photoelectric sensor (4) is located at the end of the side plate (2) of the frame away from the top plate (1) of the frame, and is used to detect the frequency of experimental animals entering the inside of the fixed frame.

2. The feeding bottle feeding frequency monitoring device of claim 1, wherein, The bottle mouth (51) is in the shape of a round tube, and the perforation (11) is a round hole that matches the shape of the bottle mouth (51).

3. The feeding frequency monitoring device for a feeding bottle according to claim 2, wherein The clamping mechanism (3) includes: A fixing nut (31) is fixedly installed on the side plate (2) of the frame; An adjusting screw (32) is movably inserted into the fixing nut (31). One end of the adjusting screw (32) extending into the inner side of the fixing frame is provided with a clamping member (33), which is used to cooperate with the clamping member (33) of another adjusting screw (32) to clamp the bottle mouth (51) when the adjusting screw (32) moves close to the bottle mouth (51).

4. The feeding frequency monitoring device for a feeding bottle according to claim 3, wherein The clamping member (33) is an arc-shaped clamping piece that matches the shape of the bottle opening (51).

5. A feeding frequency monitoring device for a feeding bottle according to any one of claims 1 to 4, wherein The photoelectric sensor (4) includes a laser emitting device (41) and a laser receiving device (42), which are respectively located at the ends of a pair of frame side plates (2) away from the frame top plate (1).

6. The feeding bottle feeding frequency monitoring device of claim 5, wherein, The photoelectric sensor (4) is an NPN type normally open three-wire laser sensor.

7. A lumbar disc degeneration experimental system comprising a current recorder, a switching power supply, characterized in that, It also includes a feeding bottle feeding frequency monitoring device as described in any one of claims 1-6, wherein the photoelectric sensor (4) is connected to the switching power supply, and the photoelectric sensor (4) further includes a contact detection circuit (7) connected to the current recorder, used to output a trigger signal to the current recorder when the photoelectric sensor (4) determines that the experimental animal has entered the inner side of the fixed frame.

8. The lumbar disc degeneration test system of claim 7, wherein, Also includes: Feeding cage (6); A water bottle (8) containing clean water is installed inside the feeding cage (6); The feeding bottle (5) is a sugar water feeding bottle (5) containing sugar water, which is installed inside the feeding cage (6) at a position higher than the water bottle (8).

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