Batch centrifugal device for centrifugal tubes

Abstract

The utility model relates to a centrifugal tube batch centrifuging device which comprises a rotatable rotating frame and a plurality of containing box bodies arranged on the side portion of the rotating frame, and containing grooves used for containing sample boxes are formed in the middles of the containing box bodies. According to the utility model, large-batch operation can be carried out, the operation is not like the traditional repeated operation of a single sample, the samples can be uniformly placed in the sample detection box and placed on the corresponding rotating frame for centrifugation, the working efficiency is improved, the flow of repeated operation of the single sample is reduced, the operation time is saved, and the labor intensity of workers is reduced. And meanwhile, the conditions of wrong taking, repeated operation or less operation and the like when the sample quantity is large are avoided, the samples are uniformly operated and transferred in the sample box, and the probability of process errors is reduced.

Description

Technical Field

[0001] This utility model relates to a batch centrifugation device for centrifuge tubes. Background Technology

[0002] In many fields such as medical testing, drug development, and biotechnology research, it is often necessary to process large numbers of samples, most of which are stored in 1.5 mL or 2.0 mL centrifuge tubes. Traditional methods rely on individual shaking and centrifugation, such as using the Qilinbell VORTEX-6 shaker and the Tiangen TGear centrifuge.

[0003] When centrifuging samples, this conventional method reveals many drawbacks when dealing with a large number of samples: on the one hand, operators need to spend a lot of time and energy repeatedly placing individual centrifuge tubes into the centrifuge for operation and then taking them out and putting them back, making the whole process cumbersome and lengthy; on the other hand, frequent individual operations greatly increase the probability of errors, making it easy to take the wrong sample, repeat the operation, or operate on the wrong sample, which not only affects the experimental progress but may even lead to deviations in the experimental results. Utility Model Content

[0004] This invention addresses the aforementioned problem by providing a batch centrifugation device for centrifuge tubes that is convenient and efficient to use.

[0005] This utility model is constructed as follows: it includes a rotatable rotating frame and a plurality of receiving boxes disposed on the side of the rotating frame, wherein the receiving box is provided with a receiving groove in the middle for placing a sample box.

[0006] Furthermore, the rotating frame is connected to the housing via a rotating shaft.

[0007] Furthermore, two stops are provided on the upper front and rear sides of the receiving box, and the two stops extend above the rotating frame.

[0008] Furthermore, sampling slots are provided on both sides of the receiving slot of the receiving box.

[0009] Furthermore, the receiving box is provided with slots at the front and back, and a locking block is provided in the slot. The rotating shaft is located between the locking block and the rotating frame, and the rotating shaft is rotatably engaged with the receiving box.

[0010] Furthermore, a connecting hole is provided in the middle of the rotating frame.

[0011] Furthermore, the rotating frame is I-shaped.

[0012] Furthermore, it also includes a centrifuge body, with a working chamber in the middle of the centrifuge body, a rotating frame disposed in the working chamber, a motor disposed on the bottom surface of the working chamber, the output shaft of the motor extending upward and connected to the rotating frame, and the motor output shaft being fixedly connected to the connecting hole of the rotating frame by a connector.

[0013] Furthermore, the connecting member is fixedly connected to the rotating frame and the motor output shaft, respectively.

[0014] Furthermore, the sample box includes a box body and a box lid. The box body has multiple fixing slots in the middle for placing centrifuge tubes. The centrifuge tube openings are covered with tube caps. The box lid is used to close the box body.

[0015] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention breaks through the limitations of traditional single sample processing and has the advantage of batch operation. It allows multiple samples to be put into a standard sample detection box and then placed on the corresponding adapter for centrifugation. Whether it is large-scale medical sample screening or multiple batches of small sample processing in drug development, it can be easily handled, greatly improving the sample processing throughput. When a large number of samples need to be centrifuged, after the reaction system is constructed, the sample is placed in the centrifuge tube and the centrifuge tube is fixed in the sample box. The sample box is fixed in the receiving slot of the receiving box body. The centrifuge is started. The motor of the centrifuge body drives the rotating frame, receiving box body and sample box to rotate for centrifugation separation, and the precipitate or supernatant is quickly obtained for subsequent analysis. (2) The present invention is conducive to improving work efficiency. After the operator performs the routine sample addition operation on the sample box, the sample box is put into the appropriate centrifuge equipment at once. There is no need to frequently operate the sample individually afterward. Compared with the traditional method, it reduces a large number of repetitive individual sample handling and placement actions, significantly saving operation time and making the entire experimental process more compact and efficient, providing strong support for the rapid advancement of scientific research projects; (3) Since the samples are handled and transferred in a unified sample box throughout the process, it avoids human errors such as mishandling, repeated operations, or missing operations during the process of repeatedly taking out and putting back individual samples. This is crucial for scientific research experiments that require high precision and high repeatability, ensuring the reliability and stability of experimental data and reducing experimental failures and resource waste caused by operational errors. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of an embodiment of the present utility model. Figure 1 ;

[0017] Figure 2 This is a schematic diagram of the structure of an embodiment of the present utility model. Figure 2 ;

[0018] Figure 3 This is a schematic diagram of the structure of an embodiment of the present utility model. Figure 3 ;

[0019] Figure 4 This is a schematic diagram of the structure of an embodiment of the present utility model. Figure 4 ;

[0020] Figure 5 This is a partial sectional view of the overall embodiment of this utility model;

[0021] Figure 6 This is an exploded view of the sample box in an embodiment of this utility model. Detailed Implementation

[0022] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0023] Example 1: As Figures 1-6 As shown, in this embodiment, a centrifuge tube batch centrifugation device is provided, including a rotatable rotating frame 1 and two receiving boxes 2 disposed on the side of the rotating frame. The receiving boxes are provided with a receiving groove 21 in the middle for placing sample boxes 5.

[0024] When centrifuging large batches of samples is required, after constructing the reaction system, place the samples in centrifuge tubes and secure the tubes in the sample container. Secure the sample container in the receiving slot of the centrifuge housing. Start the centrifuge; the centrifuge motor drives the rotating frame, receiving container, and sample container to rotate and separate the samples, quickly obtaining the precipitate or supernatant for subsequent analysis. Of course, the rotation speed and time can be adjusted according to the actual situation.

[0025] In this embodiment of the utility model, the rotating frame and the housing body are connected by a rotating shaft 3.

[0026] In this embodiment of the utility model, two stop points 4 are provided on the upper front and rear sides of the receiving box. The two stop points extend to the top of the rotating frame and are used to limit the swing angle of the receiving box.

[0027] In this embodiment of the present invention, in order to facilitate the removal of the sample box, sampling slots 22 are provided on both sides of the receiving slot of the receiving box body.

[0028] In this embodiment of the utility model, the front and rear of the receiving box are provided with slots 23, and a card block is provided in the slot. The rotating shaft is disposed between the card block and the rotating frame, and the rotating shaft is rotatably engaged with the receiving box. Specifically, the rotating shaft can be fixed to the rotating frame, and the rotating shaft is rotatably engaged with the card block.

[0029] In this embodiment of the utility model, a connecting hole 11 is provided in the middle of the rotating frame.

[0030] In this embodiment of the invention, the rotating frame is I-shaped.

[0031] In this embodiment of the utility model, the sample box includes a box body 51 and a box cover 52. The box body is provided with a plurality of fixing slots 511 for placing centrifuge tubes in the middle. The centrifuge tube openings are provided with tube caps. The box cover is used to close the box body.

[0032] Example 2: Based on Example 1, this embodiment of the present invention further includes a centrifuge body 6, a working chamber 61 in the middle of the centrifuge body, a rotating frame disposed in the working chamber, a motor 62 disposed on the bottom surface of the working chamber, the output shaft of the motor extending upward and connected to the rotating frame, and the output shaft of the motor being fixedly connected to the connecting hole of the rotating frame by a connector 63.

[0033] The aforementioned connecting parts are fixedly connected to the rotating frame and the motor output shaft, respectively.

[0034] The centrifuge body described above can be the Xiangyi H2050R high-capacity high-speed benchtop refrigerated centrifuge, which possesses powerful centrifugation capabilities. The rotating frame and sample container of this device are structurally designed to fully consider the mechanical requirements of high-speed centrifuge rotation, securely fixing the sample container within the centrifuge rotor to ensure a safe and stable centrifugation process, resulting in uniform sample processing under a consistent centrifugal force field. Its centrifugation speed can range from 500 to 5000 r / min.

[0035] For compound screening experiments in drug development, after completing the construction of the reaction system, the reaction tube (2.0 mL centrifuge tube) is placed in a 96-well sample box. The sample box is then fixed in the container of the Xiangyi H2050R high-capacity high-speed benchtop refrigerated centrifuge. The centrifuge speed (e.g., 4000 r / min) and centrifugation time (e.g., 5 minutes) are set according to the characteristics of the compound. Centrifugation is then performed to quickly obtain the precipitate or supernatant for subsequent analysis.

[0036] Example 3: Based on Example 2, the centrifugation device of this embodiment was used in conjunction with a Xiangyi large-capacity high-speed benchtop refrigerated centrifuge H2050R and a batch shaking device (Hangzhou Youning Instruments Co., Ltd.'s HD-2500 multi-tube vortex mixer) to perform batch centrifugation on 1.5 mL EP centrifuge tubes. Each tube contained 30-200 μL of nucleotide solution (i.e., primers or probes), which was thoroughly mixed and centrifuged to the bottom of the tube. The shaking conditions were set to 250 r / min × 30 s, and the centrifugation conditions were set to 3000 r / min × 30 s. 192 samples could be processed at a time, and the total duration of the shaking and centrifugation process was approximately 5 min. Therefore, the speed of this embodiment was approximately 5 min / 192 tubes.

[0037] The traditional manual method involves using a Linbell VORTEX-6 oscillator for 30 seconds (processing 2 EP tubes per cycle) and a Tiangen TGear micro centrifuge for 30 seconds (processing 8 EP tubes per cycle). The total time for the oscillating and centrifuging process is approximately 3 minutes, meaning the traditional method takes about 3 minutes per 8 tubes.

[0038] In this embodiment, 10,000 1.5 mL EP centrifuge tubes containing primer (or probe) solutions were centrifuged by shaking. This was completed within 5 hours, while the traditional method would take 63 hours. Therefore, the efficiency of this embodiment is significantly improved when centrifuging a batch of centrifuge tubes by shaking.

[0039] The rotor type of the device is a horizontal rotor.

[0040] The sample box is a 96-well centrifuge tube box, suitable for 1.5mL and 2mL EP centrifuge tubes and 1.5mL and 2mL screw cap centrifuge tubes.

[0041] This device can be used in conjunction with a batch centrifuge tube shaking device to achieve batch centrifuge tube shaking and centrifugation.

[0042] Unless otherwise stated, if any of the technical solutions disclosed in this utility model discloses a numerical range, then the disclosed numerical range is a preferred numerical range. Any person skilled in the art should understand that the preferred numerical range is merely one among many feasible numerical values ​​that has a more obvious or representative technical effect. Because there are many numerical values, it is impossible to list them all. Therefore, this utility model discloses only some numerical values ​​to illustrate the technical solutions of this utility model. Furthermore, the numerical values ​​listed above should not constitute a limitation on the scope of protection of this utility model.

[0043] If the terms "first" or "second" are used in this document to specify components, those skilled in the art should know that the use of "first" or "second" is merely for the purpose of distinguishing components in description, and unless otherwise stated, the above terms have no special meaning.

[0044] Meanwhile, if the present invention discloses or relates to the protection of mutually fixedly connected parts or structural components, then, unless otherwise stated, the fixed connection can be understood as: a detachable fixed connection (e.g., using bolts or screws), or a non-detachable fixed connection (e.g., riveting, welding). Of course, the mutually fixed connection can also be replaced by an integral structure (e.g., manufactured by casting process) (except where it is obviously impossible to use an integral forming process).

[0045] In addition, unless otherwise stated, the terms used in any of the technical solutions disclosed in this utility model to indicate positional relationships or shapes include states or shapes that are similar to, close to, or approximate with those states or shapes.

[0046] Any component provided by this utility model can be assembled from multiple individual components, or it can be a single component manufactured by a one-piece molding process.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and not to limit it; although the utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can still be made to the specific implementation of this utility model or equivalent substitutions can be made to some technical features without departing from the spirit of the technical solution of this utility model, and all such modifications and substitutions should be covered within the scope of the technical solution claimed by this utility model.

Claims

1. A batch centrifugation device for centrifuge tubes, characterized in that, It includes a rotatable rotating frame and multiple receiving boxes disposed on the side of the rotating frame, wherein the receiving box is provided with a receiving groove in the middle for placing a sample box.

2. The centrifuge tube batch centrifugation device according to claim 1, characterized in that, The rotating frame is connected to the housing via a rotating shaft.

3. The batch centrifugation device for centrifuge tubes according to claim 1, characterized in that, The upper front and rear sides of the housing are each provided with two stops, which extend to the top of the rotating frame.

4. The batch centrifugation device for centrifuge tubes according to claim 1, characterized in that, Sampling slots are provided on both sides of the receiving slot of the receiving box.

5. A batch centrifugation device for centrifuge tubes according to claim 2, characterized in that, The receiving box has slots on the front and back, and a locking block is provided in the slot. The rotating shaft is located between the locking block and the rotating frame, and the rotating shaft is rotatably engaged with the receiving box.

6. A batch centrifugation device for centrifuge tubes according to claim 1, characterized in that, A connecting hole is provided in the middle of the rotating frame.

7. A batch centrifugation device for centrifuge tubes according to claim 1, characterized in that, The rotating frame is I-shaped.

8. A batch centrifugation device for centrifuge tubes according to claim 1, characterized in that, It also includes a centrifuge body, a working chamber in the middle of the centrifuge body, a rotating frame in the working chamber, a motor in the bottom of the working chamber, the output shaft of the motor extending upward and connecting to the rotating frame, and the motor output shaft and the connecting hole of the rotating frame are fixedly connected by a connector.

9. A batch centrifugation device for centrifuge tubes according to claim 8, characterized in that, The connecting parts are fixedly connected to the rotating frame and the motor output shaft, respectively.

10. A batch centrifugation device for centrifuge tubes according to claim 1, characterized in that, The sample box includes a box body and a box lid. The box body has multiple fixing slots in the middle for placing centrifuge tubes. The centrifuge tube openings are covered with tube caps. The box lid is used to close the box body.

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