Cutting and equally-dividing device for PDX model inoculation
By designing a slicing and equalizing device for PDX model inoculation, the problem of uneven tissue slice division during PDX model inoculation was solved, achieving uniform tissue block cutting, improving experimental stability and reducing costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-12
- Publication Date
- 2026-03-06
AI Technical Summary
In existing technologies, the tissue slice cutting process during PDX model inoculation is affected by the operator's skill level, resulting in uneven slices, which affects the stability of the experiment and increases the experimental cost.
Design a slicing and equalizing device for PDX model inoculation, including a base, a clamping element, a cutting groove and a cutting blade. Through the cooperation of the clamping element and the cutting groove, the tissue slices are uniformly positioned and cut, ensuring the uniformity of the cutting process.
This method achieves uniform tissue block cutting, improves experimental stability, reduces experimental costs, and decreases the number of immunodeficient mice used.
Smart Images
Figure CN223971761U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of medical device technology, specifically relating to a slicing and equalizing device for PDX model inoculation. Background Technology
[0002] PDX model, short for patient-derived xenograft model, is a xenograft model formed by implanting tumor tissue and primary cells derived from a patient into immunodeficient mice. PDX model retains most of the characteristics of primary tumors at the histopathological, molecular biological, and genetic levels, and has good predictive value for clinical efficacy.
[0003] Whether fresh or cryopreserved and thawed, PDX tumor tissue needs to be evenly divided into small pieces with an area of 2-3 mm². This has a significant impact on the uniformity of subsequent tumor formation. Currently, most PDX models are cut manually with a blade, which can only estimate the size of the pieces. Therefore, this step is often affected by the operator's skill level, which greatly affects the uniformity of subsequent PDX tumor formation and thus the stability of the experiment. At present, most PDX models use severely immunodeficient mice for subsequent experiments, which are relatively expensive. Poor uniformity of PDX tissue inoculation not only leads to poor stability of experimental results, but also requires the use of more mice to conduct experiments, increasing experimental expenses.
[0004] Therefore, a slicing device is designed to uniformly divide tissue slices into tissue blocks of the same shape. Specifically, this slicing device is a slicing and dividing device for PDX model inoculation. Utility Model Content
[0005] To overcome the problems mentioned in the background art, the present invention adopts the following technical solution:
[0006] A slicing and equalizing device for PDX model inoculation includes: a base having a cylindrical cavity and a first cutting groove, the first cutting groove penetrating the base and overlapping a portion of the cylindrical cavity; a clamping member movably disposed within the cylindrical cavity, the outer wall of the clamping member having a second cutting groove penetrating through it, the first cutting groove and the second cutting groove merging to form a cutting channel when the clamping member enters the cylindrical cavity; and a cutting blade movably disposed within the cutting groove channel.
[0007] Furthermore, when the clamping member extends into the cylindrical cavity, the end of the clamping member facing the seat is always parallel to the bottom end of the cylindrical cavity. Therefore, the tissue slices stacked in the cylindrical cavity are subjected to uniform force when squeezed by the clamping member, and will not be displaced due to uneven force. The clamping member can move along the central axis of the cylindrical cavity to a limit position, and the clamping member does not contact the bottom end of the cylindrical cavity when it is in the limit position.
[0008] Furthermore, several of the first cutting grooves are arranged in a linear array to form a group of first cutting grooves, and the spacing between adjacent first cutting grooves is the same; there are at least two groups of first cutting grooves, and the first cutting grooves in different groups do not completely overlap; several second cutting grooves are arranged in a linear array to form a group of second cutting grooves, and the spacing between adjacent second cutting grooves is the same; there are at least two groups of second cutting grooves, and the second cutting grooves in different groups do not completely overlap.
[0009] Furthermore, it also includes a limiting arm, which is connected to the clamping member. When the clamping member extends into the cylindrical cavity, the limiting arm abuts against the outer wall of the seat. When the limiting arm abuts against the outer wall of the seat, the first cutting groove and the second cutting groove overlap one-to-one to form a number of intersecting cutting channels.
[0010] Furthermore, it also includes an actuator arm, which includes a slide rod and a tool holder connected to each other. The slide rod is slidably connected to the clamping member and drives the tool holder to move closer to or away from the base. The cutting blade is connected to the tool holder. When the tool holder moves towards the base under the drive of the slide rod, the cutting blade enters the cutting channel.
[0011] Furthermore, the clamping member includes a clamping part and a rotating part. The second cutting groove is disposed on the clamping part, the rotating part is rotatably connected to the clamping part, the rotating part is provided with a sliding groove, and the sliding rod is sleeved on the sliding groove and can reciprocate within the sliding groove.
[0012] Furthermore, the blade holder is arrayed with several blade slots. When the slide rod is connected to the clamping member, the blade slots are arranged in a linear array at the bottom of the blade holder, with adjacent blade slots having the same spacing. The blade slots are detachably connected to the cutting blade. The user can adjust the number of cutting blades on the blade holder according to cutting needs, thereby enabling the cutting of tissue blocks of different sizes.
[0013] Furthermore, the tool holder can move to a disengaged position and disengage the cutting blade from the cutting channel; the tool holder can move to a through position and allow the cutting blade to penetrate the cylindrical cavity; when the tool holder moves between the disengaged position and the through position, the slide rod is always sleeved on the slide groove.
[0014] Furthermore, the number of limiting arms is at least one, and the limiting arms and the clamping members simultaneously compress against the seat, which is sufficient to restrict most of the degrees of freedom of the clamping members; the number of sliding grooves is at least two, and the number of sliding grooves is the same as the number of sliding rods. More than one sliding rod can effectively ensure the stability of the tool holder during movement.
[0015] Furthermore, when the rotating part rotates about the pressing part, the blade holder is only in the disengaged position, thereby preventing the cutting blade from colliding with the limiting arm; when the blade holder reciprocates between the disengaged position and the through position, the pressing part and the rotating part do not rotate relative to each other, thereby preventing the cutting blade from colliding with the cutting channel.
[0016] The beneficial effects of this utility model are:
[0017] By setting up mutually compatible clamping components and cylindrical cavities, the cut tissue slices can be confined, preventing deformation during the cutting process and ensuring consistent sizes of the resulting tissue blocks. The presence of cutting channels penetrating the clamping components and the base, along with cutting blades that move within these channels, allows for efficient cutting simply by placing the blades into different channels and pushing or pulling them. This avoids the inefficiencies and errors associated with manual cutting, which rely on predicting the blade's position and controlling the cutting trajectory. Furthermore, by setting equally spaced cutting channels and grooves, users can customize the number and intervals of cutting blades on the blade holder according to the desired tissue block volume, resulting in excellent practicality. Attached Figure Description
[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:
[0019] Figure 1 A schematic diagram of the present invention when the clamping element is in the limiting position and the tool holder is in the through position;
[0020] Figure 2 This is a schematic diagram of the exploded structure of this utility model;
[0021] Figure 3 for Figure 2 A schematic diagram of the cross-sectional structure;
[0022] Figure 4 for Figure 1 A schematic diagram of the structure of this utility model when the central tool holder moves to the disengaged position;
[0023] Figure 5 for Figure 4 A schematic diagram of the structure of this utility model showing the central rotating part rotating and driving the cutting blade to align with another set of cutting grooves;
[0024] Figure 6 for Figure 5A schematic diagram of the structure of this utility model when the central tool holder moves to the through position;
[0025] Figure 7 for Figure 6 A schematic diagram of the cross-sectional structure;
[0026] Figure 8 This is a schematic diagram of the actuator arm.
[0027] Figure 9 This is a schematic diagram of the structure when the clamping part is connected to the two limiting arms;
[0028] In the figure, 1 is the base; 11 is the cylindrical cavity; 2 is the cutting channel; 21 is the first cutting groove; 22 is the second cutting groove; 3 is the clamping part; 31 is the clamping part; 32 is the rotating part; 321 is the sliding groove; 4 is the limiting arm; 5 is the actuating arm; 51 is the sliding rod; 52 is the tool holder; 521 is the tool groove; and 53 is the cutting tool. Detailed Implementation
[0029] The technical solutions of this utility model are clearly and completely described below through specific embodiments. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0030] A dicing and equalizing device for PDX model inoculation, such as Figure 1-9As shown, the device includes: a base 1 with a cylindrical cavity 11 and a cutting groove 21, the cutting groove 21 penetrating the base 1 and overlapping part of the cylindrical cavity 11; a clamping member 3, movably disposed within the cylindrical cavity 11, with a cutting groove 22 penetrating its outer wall; when the clamping member 3 enters the cylindrical cavity 11, the cutting groove 21 and the cutting groove 22 overlap to form a cutting channel 2; and a cutting blade 53, movably disposed within the cutting groove channel. The vertical cross-section of the cylindrical cavity 11 along the axial direction is one of a circle, rectangle, or regular polygon. The cylindrical cavity 11 can fit with the clamping member 3, and the height of the clamping member 3 is greater than the depth of the cylindrical cavity 11 to facilitate the operator in removing the clamping member 3 from the cylindrical cavity 11. The base 1 is made of one of the following materials: 316 stainless steel, 304 stainless steel, PPS, PC, PP or PTFE. The cutting blade 53 is made of the same material as the base 1. This gives the cutting blade 53 and the base 1 excellent corrosion resistance while also providing the hardness to cut tumor slices, which helps to improve the service life of the dicing and equalizing device.
[0031] In some embodiments of this application, such as Figure 1-9 As shown, when the clamping member 3 extends into the cylindrical cavity 11, the end of the clamping member 3 facing the seat 1 is always parallel to the bottom end of the cylindrical cavity 11. Therefore, the tissue slices stacked in the cylindrical cavity 11 are subjected to uniform force when squeezed by the clamping member 3, and will not be displaced due to uneven force. The clamping member 3 can move along the central axis of the cylindrical cavity 11 to a limit position. When the clamping member 3 is in the limit position, it does not contact the bottom end of the cylindrical cavity 11. When the operator uses the clamping member 3 to limit the tissue slices, he cannot see the situation inside the cylindrical cavity 11 directly. When the clamping member 3 moves to the limit position in the cylindrical cavity 11, it cannot continue to approach the bottom end of the cylindrical cavity 11, so that there is always a cylindrical space in the cylindrical cavity 11, which helps to prevent the clamping member 3 from applying too much pressure and damaging the tissue slices.
[0032] In some embodiments of this application, such as Figure 1-9As shown, several cutting grooves 21 are arranged in a linear array to form a group of cutting grooves 21. The spacing between adjacent cutting grooves 21 is the same, and the spacing between adjacent cutting grooves 21 is not less than 1 mm. There are at least two groups of cutting grooves 21, and the cutting grooves 21 in different groups do not completely overlap. Several cutting grooves 22 are arranged in a linear array to form a group of cutting grooves 22. The spacing between adjacent cutting grooves 22 is the same, and the spacing between adjacent cutting grooves 21 is not less than 1 mm. There are at least two groups of cutting grooves 22, and the cutting grooves 22 in different groups do not completely overlap. This causes the blade to form intersecting trajectories when moving within the different groups of cutting grooves 21, and these trajectories cut several identical polygonal patterns on the tissue slice, thereby dividing the tissue slice with uniform thickness into tissue blocks of the same volume. There is an included angle between different groups of cutting grooves 21. The value of the included angle is related to the number of groups of cutting grooves 21. If the number of cutting grooves 21 is defined as a, then the included angle between adjacent groups of cutting grooves 21 is 360° / 2a. Therefore, when there are two groups of cutting grooves 21, the included angle between adjacent groups of cutting grooves 21 is 90°. The same applies to cutting groove 22.
[0033] In some embodiments of this application, such as Figure 1-9 As shown, it also includes a limiting arm 4, which is connected to the clamping member 3. When the clamping member 3 extends into the cylindrical cavity 11, the limiting arm 4 abuts against the outer wall of the base 1. When the limiting arm 4 abuts against the outer wall of the base 1, the cutting groove 1 21 and the cutting groove 22 overlap one-to-one to form several intersecting cutting channels 2. The inner side of the limiting arm 4 is parallel to the outer wall of the base 1, which allows the user to complete the connection operation between the limiting arm 4 and the base 1 at the same time as inserting the clamping member 3 into the cylindrical cavity 11. This not only enhances the connection stability between the clamping member 3 and the base 1, but also simplifies the complexity of the operation.
[0034] In some embodiments of this application, such as Figure 1-9As shown, the device also includes an actuator arm 5, which comprises a slide rod 51 and a tool holder 52 connected to each other. The slide rod 51 is slidably connected to the clamping member 3 and drives the tool holder 52 to move closer to or away from the base 1. The cutting blade 53 is connected to the tool holder 52. When the tool holder 52 moves towards the base 1 under the action of the slide rod 51, the cutting blade 53 enters the cutting channel 2. The tool holder 52 has several slots 521 arranged in an array. When the slide rod 51 is connected to the clamping member 3, the slots 521 are arranged in a linear array at the bottom of the tool holder 52. The spacing between adjacent slots 521 is the same, and the spacing between the slots 521 is the same as the spacing between cutting slot 1 21 or cutting slot 22. The blade groove 521 is detachably connected to the cutting blade 53. Specifically, the blade groove 521 is elastic and can hold the inserted blade; or, the spacing between the inner walls of the blade groove 521 is the same as the thickness of the blade back of the cutting blade 53, which allows the cutting blade 53 to slide into the blade groove 521 during assembly and be pressed and fixed by the inner wall of the blade groove 521. The user can adjust the number of cutting blades 53 on the blade holder 52 according to the cutting needs, thereby being able to cut tissue blocks of different sizes. The blade holder 52 can move to a disengaged position and disengage the cutting blade 53 from the cutting channel 2; the blade holder 52 can move to a through position and allow the cutting blade 53 to penetrate the cylindrical cavity 11; when the blade holder 52 moves between the disengaged position and the through position, the slide rod 51 is always sleeved in the slide groove 321.
[0035] In some embodiments of this application, such as Figure 1-9 As shown, the clamping member 3 includes a clamping part 31 and a rotating part 32. A cutting groove 22 is disposed on the clamping part 31, and the rotating part 32 is rotatably connected to the clamping part 31. The rotating part 32 is provided with a sliding groove 321, and the sliding rod 51 is sleeved in the sliding groove 321 and can reciprocate within the sliding groove 321. The number of sliding grooves 321 is at least two, and the number of sliding grooves 321 is the same as the number of sliding rods 51. More than one sliding rod 51 can effectively ensure the stability of the tool holder 52 during movement.
[0036] In some embodiments of this application, such as Figure 1-9 As shown, there is at least one limiting arm 4. The limiting arm 4 and the clamping member 3 simultaneously compress against the seat 1, which is sufficient to restrict most of the degrees of freedom of the clamping member 3, leaving the clamping member 3 with only the degree of freedom to reciprocate along the axial direction of the cylindrical cavity 11. Too many limiting arms 4 would restrict the movement of the cutting blade 53 and the number of cutting blades 53 in each group. When the rotating part 32 rotates about the clamping part 31, the blade holder 52 is only in the disengaged position, thereby preventing the cutting blade 53 from colliding with the limiting arm 4. When the blade holder 52 reciprocates between the disengaged position and the through position, the clamping part 31 and the rotating part 32 do not rotate relative to each other, thereby preventing the cutting blade 53 from colliding with the cutting channel 2.
Claims
1. A device for uniform division of a cut piece for PDX model inoculation, characterized by, The utility model provides a cutting device, including the seat body that is opened with the cylindrical cavity and cutting slot one, cutting slot one penetrates the seat body and coincides with part cylindrical cavity, the pressure part can move setting in the cylindrical cavity, the outer wall of pressure part is provided with cutting slot two and penetrates, cutting slot one and cutting slot two coincide and constitute cutting channel when pressure part enters cylindrical cavity, Cutting knife can move setting in cutting channel.
2. The device for uniform division of a cut piece for PDX model inoculation according to claim 1, characterized in that, When the pressure part extends into the cylindrical cavity, the end of the pressure part towards the seat body is always parallel to the bottom end of the cylindrical cavity, the pressure part can move along the central axis of the cylindrical cavity to a limit position, and the pressure part does not contact the bottom end of the cylindrical cavity when it is in the limit position.
3. The device for uniform division of a cut piece for PDX model inoculation according to claim 1, characterized in that, A plurality of cutting slots one are linearly arrayed and constitute a group of cutting slots one, and the spacing between adjacent cutting slots one is the same. The cutting slots one are provided with at least two groups, and the cutting slots one of different groups do not completely coincide. A plurality of cutting slots two are linearly arrayed and constitute a group of cutting slots two, and the spacing between adjacent cutting slots two is the same. The cutting slots two are provided with at least two groups, and the cutting slots two of different groups do not completely coincide.
4. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 3, wherein, It also includes a limiting arm connected to the pressure part, the limiting arm abuts against the outer wall of the seat body when the pressure part extends into the cylindrical cavity, and the cutting slot one and the cutting slot two coincide one by one and form a plurality of intersecting cutting channels when the limiting arm abuts against the outer wall of the seat body.
5. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 4, wherein It also includes an execution arm including a sliding rod and a knife holder connected to each other, the sliding rod can be slidingly connected with the pressure part and drive the knife holder to approach or move away from the seat body, the cutting knife is connected with the knife holder, and the cutting knife enters the cutting channel when the knife holder moves towards the seat body under the drive of the sliding rod.
6. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 5, wherein, The pressure part includes a pressing part and a rotating part, the cutting slot two is provided on the pressing part, the rotating part is rotationally connected with the pressing part, the rotating part is provided with a sliding groove, and the sliding rod is sleeved on the sliding groove and can reciprocate in the sliding groove.
7. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 5, wherein A plurality of knife grooves are linearly arrayed on the knife holder, the knife grooves are linearly arrayed at the bottom end of the knife holder when the sliding rod is connected with the pressure part, the spacing between adjacent knife grooves is the same, and the knife grooves are detachably connected with the cutting knife.
8. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 6, wherein The knife holder can move to a disengagement position and make the cutting knife disengage from the cutting channel, the knife holder can move to a penetration position and make the cutting knife penetrate the cylindrical cavity, and the sliding rod is always sleeved on the sliding groove when the knife holder moves between the disengagement position and the penetration position.
9. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 6, wherein, The number of limiting arms is at least one, and the number of sliding grooves is at least two, which is consistent with the number of sliding rods.
10. The apparatus for uniform division of a cut piece for PDX model inoculation according to claim 8, wherein, When the rotating part rotates relative to the pressing part, the knife holder is only in the disengagement position, and the pressing part and the rotating part do not rotate relative to each other when the knife holder reciprocates between the disengagement position and the penetration position.