Solid-phase synthesizer for synthesizing small nucleic acid with high purity

Abstract

The utility model discloses a small nucleic acid solid phase synthesizer with high synthesis purity, which comprises an instrument main body, the outer surface of the instrument main body is provided with a mounting groove, the inner bottom wall of the mounting groove is fixedly connected with a spring, the top end of the spring is fixedly connected with a bottom bottle rack, and the inner wall of the upper surface of the bottom bottle rack is fixedly connected with a reagent bottle main body. According to the small nucleic acid solid-phase synthesizer with high synthesis purity, when a reagent bottle is placed, the bottom bottle rack and the compression spring downwards slide in the bottom groove, then the bottom end of the reagent bottle is placed in a hole in the upper surface of the bottom bottle rack, the reagent bottle is vertically arranged in the mounting groove, a communicating pipe and an air pump pipe are placed in the reagent bottle, and the reagent bottle is placed in the top seat. Then the spring is slowly reset, the top end of the reagent bottle enters a hole in the lower surface of the bottle blocking cover, the reagent bottle is fixed between the bottom bottle rack and the bottle blocking cover, the bottom bottle rack is also slid downwards to enable the top end of the bottom bottle rack to leave the bottle blocking cover, and the reagent bottle can be taken away.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid synthesis equipment technology, and in particular to a solid-phase synthesizer for small nucleic acids with high synthesis purity. Background Technology

[0002] Artificial nucleic acid synthesis is the only known method for targeted modification of gene sequences, and it is widely used in many fields such as protein modification and life sciences, including nucleic acid drugs, enzyme engineering, gene detection, and gene therapy. In the synthesis process, various reaction reagents are sequentially injected into a synthesis column to react with the carrier within the column. With the rapid development of oligonucleotide chain synthesis technology, synthesis costs have continuously decreased, and synthesis length and precision have continuously improved, making large-scale DNA and RNA synthesis starting from oligonucleotide chains possible. Therefore, nucleic acid synthesizers have emerged.

[0003] Existing nucleic acid synthesizers can only hold reagent bottles of a fixed length and cannot adjust the position of the bottle rack according to the height of the reagent bottles, thus failing to meet the needs of users and having poor applicability. Therefore, there is a need for a small nucleic acid solid-phase synthesizer that can accommodate reagent bottles of different lengths. Utility Model Content

[0004] The main objective of this invention is to provide a solid-phase synthesizer for small nucleic acids with high synthesis purity, which can effectively solve the problems in the background technology.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0006] A solid-phase synthesizer for synthesizing small nucleic acids with high purity includes an instrument body. An installation groove is formed on the outer surface of the instrument body. A spring is fixedly connected to the inner bottom wall of the installation groove. A bottom bottle holder is fixedly connected to the top of the spring. A reagent bottle body is fixedly connected to the inner wall of the upper surface of the bottom bottle holder. A top seat is fixedly connected to the upper surface of the instrument body. A bottle cap is fixedly connected to the inner wall of the top seat. A connecting tube is fixedly connected to the inner wall of the upper surface of the bottle cap. An air pump tube is fixedly connected to the inner inner wall of the upper surface of the bottle cap. A synthesis column is fixedly connected to the inner top wall of the instrument body.

[0007] In order to achieve the effect of sealing the bottom groove opening, as a small nucleic acid solid phase synthesizer with high synthesis purity according to this utility model, the instrument body is provided with a bottom groove inside, and the outer surface of the instrument body is hinged with a sealing door.

[0008] To facilitate the up-and-down movement of the bottom bottle holder, as a small nucleic acid solid-phase synthesizer with high synthesis purity according to this utility model, the inner wall of the mounting groove is fixedly connected with a slide rail, and the outer surface of the bottom bottle holder is provided with a slide groove.

[0009] To facilitate the delivery of reagent solutions into the mixing chamber, this invention provides a solid-phase synthesizer for synthesizing small nucleic acids with high purity. The upper surface of the instrument body is provided with a mixing chamber, and a collection frame is fixedly connected to the top of the inner wall of the mixing chamber.

[0010] In order to achieve the effect of mixing various reagent solutions, as a small nucleic acid solid-phase synthesizer with high synthesis purity according to this utility model, a mixing cylinder is fixedly connected to the inner wall of the mixing chamber, and a spiral blade is fixedly connected to the inner wall of the mixing cylinder.

[0011] In order to facilitate the flow of liquid into the synthesis column, as a solid-phase synthesizer for small nucleic acids with high synthesis purity according to this utility model, a liquid outlet hopper is fixedly connected to the inner wall of the lower surface of the mixing cylinder, and a liquid outlet is opened on the inner bottom wall of the mixing chamber.

[0012] In order to achieve the effect of sealing the mixing chamber, as a small nucleic acid solid-phase synthesizer with high synthesis purity according to this utility model, a sealing cover is fixedly connected to the upper surface of the instrument body, and a tube hole is opened on the upper surface of the sealing cover.

[0013] To facilitate the control of pipeline opening and closing, as a solid-phase synthesizer for synthesizing high-purity small nucleic acids according to this utility model, an electromagnetic valve is fixedly connected to the upper surface of the bottle cap, and an electromagnetic valve is fixedly connected to the inner top wall of the instrument body.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. This high-purity small nucleic acid solid-phase synthesizer, through the arrangement of the instrument body, mounting slot, spring, bottom bottle rack, top seat, bottle cap, connecting tube, air pump tube, and synthesis column, allows for precise placement of reagent bottles. First, slide the bottom bottle rack downwards in the bottom slot to compress the spring. Then, place the bottom end of the reagent bottle into the hole on the upper surface of the bottom bottle rack, ensuring the bottle is vertical in the mounting slot. Place the connecting tube and air pump tube into the reagent bottle. Slowly return the spring to its original position, allowing the top of the reagent bottle to enter the hole on the lower surface of the bottle cap, thus securing the bottle between the bottom bottle rack and the bottle cap. Removing the reagent bottle involves sliding the bottom bottle rack downwards until the top of the rack leaves the bottle cap, allowing the bottle to be removed. This allows for the installation of reagent bottles of different lengths within the instrument for nucleic acid synthesis, meeting user needs and improving instrument applicability.

[0016] 2. This high-purity small nucleic acid solid-phase synthesizer, through the configuration of a collection frame, mixing cylinder, spiral blades, outlet hopper, and outlet, allows for efficient synthesis. After the air pump tube and air pump are connected and the reagent bottles are fixed in place, the solenoid valves at the corresponding reagent bottles and synthesis column are opened, and the air pump is started. Inert gas enters the reagent bottles through the air pump tube, sending the solutions inside the bottles into the mixing chamber through the connecting tube. The various reagent solutions entering the mixing chamber flow in the spiral blades within the mixing cylinder, causing them to slowly mix together during the flow. Then, the solutions flow into the synthesis column from the outlet for solid-phase conversion. Thus, the designed mixing channel can mix the reagent solutions, ensuring the purity of the subsequent nucleic acid synthesis. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a solid-phase synthesizer for synthesizing small nucleic acids with high purity, as described in Embodiment 1 of this utility model.

[0018] Figure 2 This is a bottom-view isometric structural diagram of a solid-phase synthesizer for synthesizing high-purity small nucleic acids according to Embodiment 1 of this utility model;

[0019] Figure 3 This is an isometric structural diagram of the main body of a solid-phase synthesizer for synthesizing small nucleic acids with high purity, according to Embodiment 1 of this utility model.

[0020] Figure 4 This is an isometric view of the top seat in a solid-phase synthesizer for synthesizing small nucleic acids with high purity, according to Embodiment 1 of this utility model.

[0021] Figure 5 This is a frontal cross-sectional view of a solid-phase synthesizer for synthesizing high-purity small nucleic acids, according to Embodiment 1 of this utility model.

[0022] Figure 6 This is a schematic cross-sectional view of the right side of a solid-phase synthesizer for synthesizing high-purity small nucleic acids, according to Embodiment 1 of this utility model.

[0023] Figure 7 This is a cross-sectional view of the bottom bottle rack in a solid-phase synthesizer for synthesizing small nucleic acids with high purity, according to Embodiment 1 of this utility model.

[0024] In the diagram: 1. Instrument body; 2. Mounting slot; 3. Spring; 4. Bottom bottle holder; 5. Top seat; 6. Bottle cover; 7. Connecting tube; 8. Air pump tube; 9. Synthesis column; 10. Bottom groove; 11. Reagent bottle body; 12. Slide rail; 13. Slide groove; 14. Liquid collection frame; 15. Mixing cylinder; 16. Spiral blade; 17. Dispensing funnel; 18. Dispensing port; 19. Sealing cap; 20. Tube hole; 21. Sealing door; 22. Mixing chamber. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model. Example

[0026] like Figure 1-7 As shown, a solid-phase synthesizer for synthesizing small nucleic acids with high purity includes an instrument body 1. An installation groove 2 is formed on the outer surface of the instrument body 1. A spring 3 is fixedly connected to the inner bottom wall of the installation groove 2. A bottom bottle holder 4 is fixedly connected to the top of the spring 3. A reagent bottle body 11 is fixedly connected to the inner wall of the upper surface of the bottom bottle holder 4. A top seat 5 is fixedly connected to the upper surface of the instrument body 1. A bottle cover 6 is fixedly connected to the inner wall of the top seat 5. A connecting tube 7 is fixedly connected to the inner wall of the upper surface of the bottle cover 6. An air pump tube 8 is fixedly connected to the inner wall of the upper surface of the bottle cover 6. A synthesis column 9 is fixedly connected to the inner top wall of the instrument body 1.

[0027] In practical use, the instrument body 1, mounting slots 2, springs 3, bottom bottle holders 4, top seat 5, bottle caps 6, connecting pipes 7, air pump pipes 8, and synthesis column 9 are arranged as follows: The instrument body 1 has eight mounting slots 2, each containing a spring 3 and a bottom bottle holder 4. The bottom bottle holder 4 can slide within the mounting slot 2. The bottom of the reagent bottle body 11 is placed inside the bottom bottle holder 4. The top seat 5 above the instrument body 1 contains eight bottle caps 6 corresponding to the mounting slots 2. The top of the reagent bottle body 11 is located inside the bottle caps 6. The top of the air pump pipe 8 is connected to an air pump filled with inert gas, and its bottom end extends into the bottom of the reagent bottle, connecting to a solenoid valve at the bottle cap 6. One end of the connecting pipe 7 is located inside the reagent bottle body 11, and the other end passes through the sealing cap 19 and is located inside the mixing chamber 22. It is also connected to another solenoid valve at the bottle cover 6. The top of the synthesis column 9 is connected to a solenoid valve, which is connected to the outlet 18. When placing the reagent bottle, first slide the bottom bottle rack 4 downward in the bottom groove 10 to compress the spring 3. Then, put the bottom end of the reagent bottle into the hole on the upper surface of the bottom bottle rack 4 so that the reagent bottle is vertical in the mounting groove 2. Then, put the connecting tube 7 and the air pump tube 8 into the reagent bottle. Then, slowly let the spring 3 return to its original position. The top of the reagent bottle enters the hole on the lower surface of the bottle cover 6, fixing the reagent bottle between the bottom bottle rack 4 and the bottle cover 6. To remove the reagent bottle, slide the bottom bottle rack 4 downward so that the top of the bottom bottle rack 4 leaves the bottle cover 6, and then the reagent bottle can be removed. In this way, reagent bottles of different lengths can be installed in the instrument for nucleic acid synthesis, meeting the needs of users and improving the applicability of the instrument.

[0028] In this embodiment, the instrument body 1 has a bottom groove 10 inside, and a closed door 21 is hinged to the outer surface of the instrument body 1.

[0029] In practical use, the opening of the bottom groove 10 is closed by setting the closing door 21.

[0030] In this embodiment, a slide rail 12 is fixedly connected to the inner wall of the mounting groove 2, and a slide groove 13 is provided on the outer surface of the bottom bottle holder 4.

[0031] In practical use, the slide rail 12 and slide groove 13 facilitate the up and down movement of the bottom bottle holder 4.

[0032] In this embodiment, a mixing chamber 22 is provided on the upper surface of the instrument body 1, and a liquid collection frame 14 is fixedly connected to the top of the inner wall of the mixing chamber 22.

[0033] In practical use, the liquid collection frame 14 facilitates the delivery of reagent solution into the mixing cylinder 15.

[0034] In this embodiment, a mixing cylinder 15 is fixedly connected to the inner wall of the mixing chamber 22, and a spiral blade 16 is fixedly connected to the inner wall of the mixing cylinder 15.

[0035] In practical use, various reagent solutions are mixed using the mixing cylinder 15.

[0036] In this embodiment, a liquid outlet 17 is fixedly connected to the inner wall of the lower surface of the mixing cylinder 15, and a liquid outlet 18 is provided on the inner bottom wall of the mixing chamber 22.

[0037] In practical use, the liquid outlet 18 is designed to facilitate the flow of liquid into the synthesis column 9.

[0038] In this embodiment, a sealing cover 19 is fixedly connected to the upper surface of the instrument body 1, and a tube hole 20 is opened on the upper surface of the sealing cover 19.

[0039] In practical use, the mixing chamber 22 is sealed by setting the sealing cover 19.

[0040] In this embodiment, a solenoid valve is fixedly connected to the upper surface of the bottle cover 6, and a solenoid valve is fixedly connected to the inner top wall of the instrument body 1.

[0041] In practical use, the solenoid valve allows for convenient control of the opening and closing of the pipeline.

[0042] Working principle: When placing the reagent bottle, first slide the bottom bottle holder 4 downwards in the bottom groove 10 to compress the spring 3. Then, place the bottom end of the reagent bottle into the hole on the upper surface of the bottom bottle holder 4, making the reagent bottle vertical in the mounting groove 2. Insert the connecting tube 7 and the air pump tube 8 into the reagent bottle. Then, slowly return the spring 3 to its original position, and the top of the reagent bottle enters the hole on the lower surface of the bottle stop 6, fixing the reagent bottle between the bottom bottle holder 4 and the bottle stop 6. To remove the reagent bottle, slide the bottom bottle holder 4 downwards to remove the top of the bottom bottle holder 4 from the bottle stop 6. After removing the reagent bottle and connecting it to the air pump tube 8, and securing the reagent bottle, open the solenoid valves at the corresponding reagent bottle and the synthesis column 9, start the air pump, and inert gas enters the reagent bottle from the air pump tube 8, sending the solution inside the bottle into the mixing chamber 22 through the connecting tube 7. The various reagent solutions entering the mixing chamber 22 flow in the spiral blades 16 inside the mixing cylinder 15, causing the various reagent solutions to slowly mix together during the flow. Then, they flow into the synthesis column 9 from the outlet 18 for solid-phase conversion to synthesize nucleic acids.

[0043] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A solid phase synthesizer for small nucleic acids with high purity of synthesis, comprising an instrument body (1), characterized in that: The outer surface of the instrument body (1) is provided with a mounting groove (2), the inner bottom wall of the mounting groove (2) is fixedly connected with a spring (3), the top end of the spring (3) is fixedly connected with a bottom bottle rack (4), the inner wall of the upper surface of the bottom bottle rack (4) is fixedly connected with a reagent bottle body (11), the upper surface of the instrument body (1) is fixedly connected with a top seat (5), the inner wall of the top seat (5) is fixedly connected with a bottle cover (6), the inner wall of the upper surface of the bottle cover (6) is fixedly connected with a communication pipe (7), the inner wall of the upper surface of the bottle cover (6) is fixedly connected with a gas pump pipe (8), and the inner top wall of the instrument body (1) is fixedly connected with a synthesis column (9).

2. The solid phase synthesizer for small nucleic acids with high purity according to claim 1, characterized in that: The inside of the instrument body (1) is provided with a bottom groove (10), and the outer surface of the instrument body (1) is hingedly connected with a closed door (21) through a hinge.

3. The solid phase synthesizer for small nucleic acids with high purity according to claim 1, characterized in that: The inner wall of the mounting groove (2) is fixedly connected with a sliding rail (12), and the outer surface of the bottom bottle rack (4) is provided with a sliding groove (13).

4. The solid phase synthesizer for small nucleic acids with high purity according to claim 1, characterized in that: The upper surface of the instrument body (1) is provided with a mixed liquid cavity (22), and the inner wall top of the mixed liquid cavity (22) is fixedly connected with a liquid collecting frame (14).

5. The high-purity small nucleic acid solid-phase synthesizer according to claim 4, characterized in that: The inner wall of the mixed liquid cavity (22) is fixedly connected with a mixed liquid cylinder (15), and the inner wall of the mixed liquid cylinder (15) is fixedly connected with a spiral blade (16).

6. The high-purity small nucleic acid solid-phase synthesizer according to claim 5, characterized in that: The inner wall of the lower surface of the mixed liquid cylinder (15) is fixedly connected with a liquid outlet (17), and the inner bottom wall of the mixed liquid cavity (22) is provided with a liquid outlet (18).

7. The solid phase synthesizer for small nucleic acids according to claim 1, wherein: The upper surface of the instrument body (1) is fixedly connected with a closed cover (19), and the upper surface of the closed cover (19) is provided with a pipe hole (20).

8. The high-purity small nucleic acid solid-phase synthesizer according to claim 1, characterized in that: The upper surface of the bottle cover (6) is fixedly connected with a solenoid valve, and the inner top wall of the instrument body (1) is fixedly connected with a solenoid valve.

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