Multifunctional detection sample accommodating device and multifunctional detection device

Abstract

Each of the multifunctional detection sample accommodating device and the multifunctional detection device comprises a detection part A and a detection part B; the detection part A comprises a cavity A; the cavity A is used for accommodating a sample A, the cavity A is used for shooting a microscopic magnification image of a microscopic examination sample, and the microscopic magnification image is used for visible component analysis; the detection part B comprises a cavity B; the cavity B is used for accommodating a sample B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for detection-free analysis of the sample B. The multifunctional detection device comprises a microscopic amplification digital imaging assembly and an optical detection assembly, the microscopic magnification digital imaging assembly is used for shooting a microscopic magnification image of the microscopic examination sample; and obtaining an image of the sample in the cavity A for visible component analysis. Light in the optical detection assembly irradiates a sample in a cavity B of the external multifunctional detection device, and detection-free analysis of the sample is carried out according to optical reaction characteristics of the sample.

Description

Technical Field

[0001] This application belongs to the field of medical device technology, and in particular relates to a multifunctional sample container for testing. Background Technology

[0002] Medical testing analytical devices include a variety of instruments, which can be classified in various ways according to their function or detection principle, such as:

[0003] Type A: Detection devices based on the optical reaction characteristics of samples, such as flow cytometers and biochemical analysis instruments. These devices detect light energy based on the transmission, scattering, or absorption characteristics of different substances in the sample, thereby obtaining information about the composition or content of the corresponding components.

[0004] Type B: Devices for the quantitative and qualitative detection of trace amounts of immunomarkers. These instruments operate on complex principles and include enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, immunoturbidimetric assay, immunofluorescence assay, and chemiluminescence immunoassay. Most utilize optical properties for detection. Type C: Devices based on microscopic magnification for the analysis of formed elements. Examples include blood analyzers for detecting white blood cells and red blood cells, and urine analyzers for detecting crystalline components in urine.

[0005] Patients entering the hospital need to undergo various tests and use different instruments, which takes a long time and is costly.

[0006] With the development of the pet economy, pet hospitals have increasingly higher requirements for high-end equipment. However, pet hospitals are sensitive to the cost of equipment investment, and at the same time, the area of ​​pet hospitals is limited, making it difficult to accommodate a large number of instruments and equipment.

[0007] The applicant has filed a series of Chinese patents, such as

[0008] 1. CN2020112669290, "Cellular analysis methods and systems and quantitative methods and systems";

[0009] 2. CN2022104799126, "A method for rapid focusing of a microscopic image acquisition device and a method for detecting microscopic images";

[0010] 3. CN2023100423151, "Blood Imaging Analysis System and Method".

[0011] Using a brand-new technological solution, artificial intelligence is used to measure the content of formed elements in blood samples.

[0012] In practice, traditional tests, such as hematocrit and immunoassay, require additional instruments. Therefore, how to reduce the types and number of samples collected during the testing process, and how to complete multiple tests in one test, especially tests across sample types and testing principles in one test, are the technical problems that this application needs to solve. Summary of the Invention

[0013] This application proposes that the multifunctional sample container device includes both a sample container part that is flattened and suitable for microscopic digital imaging, and a sample container part that is suitable for light to pass through and detect, so that the sample container device can be used in multifunctional detection scenarios.

[0014] The solution to the above-mentioned technical problems is a multifunctional sample containing device, including a detection unit A and a detection unit B; the detection unit A includes a cavity A; the cavity A is used to contain sample A, and the cavity A is used to capture a magnified microscopic image of the microscopic sample, the magnified microscopic image being used for formed element analysis; the detection unit B includes a cavity B; the cavity B is used to contain sample B, and the cavity B is used for optical detection and analysis of the sample, the optical detection and analysis being used for immunoassay analysis of sample B.

[0015] It is possible that the detection unit B also includes a sample inlet B; the cavity B is connected to the sample inlet B; the detection unit A also includes a sample inlet A; the cavity A is connected to the sample inlet A.

[0016] It may also include a reagent container C, which includes a cavity C and a sample dispensing port C. The cavity C and the sample dispensing port C are connected. The cavity C is used to contain the test reagent. The sample dispensing port C opens upward, and liquid is added to or removed from the cavity C through the sample dispensing port C.

[0017] It is possible that the cavity C described above is used to contain staining reagents; or that the cavity C described above is used to contain immunoassay reagents or biochemical assay reagents.

[0018] It is possible that the aforementioned immunoassay reagent or biochemical assay reagent includes detection microparticles, the surface of which includes antigens or antibodies. When the aforementioned detection microparticles are added to sample B, the detection microparticles aggregate, causing changes in the optical properties of sample B.

[0019] It may also include a sample preparation unit D, which includes a cavity D and a sample dispensing port D, and the cavity D is connected to the sample dispensing port D; the cavity D is used for the preparation of sample A; or the cavity D is used for the preparation of sample B.

[0020] It is permissible that the bottom of the aforementioned cavity C is higher than the aforementioned sample inlet A.

[0021] It is permissible that the bottom of the aforementioned cavity C is lower than the aforementioned sample inlet A.

[0022] It is possible that detection unit A and detection unit B are located on the same side of the multifunctional detection sample holding device.

[0023] It is possible that detection unit A and detection unit B are located on different sides of the multifunctional sample container.

[0024] Alternatively, the bottom of cavity C could be at the same height as the bottom of cavity D.

[0025] The solution to the above-mentioned technical problems in this application can also be a multifunctional detection device, used in conjunction with the aforementioned multifunctional detection sample containing device, including a microscopic magnification digital imaging component and an optical detection component; the microscopic magnification digital imaging component is used to capture microscopic magnified images of the microscopically examined sample; to obtain an image of the sample in cavity A for formed element analysis; the light in the optical detection component irradiates the sample in cavity B of the external multifunctional detection device, and the sample is subjected to immune detection and quarantine analysis based on the optical reaction characteristics of the sample.

[0026] The aforementioned optical detection component includes a light source and a light detection component; the light source illuminates the aforementioned cavity B, and the light detection component acquires the light passing through the cavity B to obtain the optical reaction characteristics information of the sample.

[0027] The technical advantages of the above solution are as follows: Cavity A, the formed element detection cavity, is used to capture magnified microscopic images of the microscopic sample; Cavity B, the optical detection cavity, is used for optical detection and analysis of the sample. This makes the sample containment device suitable for different application scenarios, including comprehensive applications. It can be used for microscopic digital imaging analysis of formed elements, as well as for biochemical and immunological detection analysis. The material of Cavity B meets the corresponding optical characteristic requirements and can be made of materials with different optical properties, such as glass, sapphire, or transparent plastic.

[0028] The technical effect of the above solution is that the reagent container C, like the detection container B, can increase the capacity for one more detection sample.

[0029] The technical effect of the above technical solution is that the cavity C in the reagent containing part C is used to contain staining reagents; or the cavity C is used to contain immunoassay reagents or biochemical assay reagents; providing storage space for reagents in the process, so that the reagent and sample containing device can be integrated.

[0030] The technical effect of the above solution is: based on the detection of particle aggregation, which causes changes in the optical properties of sample B, optical feature detection is performed to achieve immunoassay or biochemical detection.

[0031] The technical effect of the above technical solution is that the cavity D in the sample preparation section D provides space for sample preparation, and the same sample holding device can support the entire process of reagent packaging, sample preparation and sample detection.

[0032] The technical advantages of the above solution are: detection unit A and detection unit B are located on the same side or different sides of the multifunctional detection sample containing device, which facilitates the setting of different detection components, and has better adaptability and flexibility.

[0033] The technical effect of the above technical solution is that the bottom of cavity C is higher than the sample inlet A, which facilitates the manufacturing of cavity C and allows for direct combination and stacking.

[0034] The technical effect of the above-mentioned technical solution is that the bottom of the cavity C is lower than the sample inlet A, so that the volume of the cavity C can be as large as possible at the same height, and can accommodate more samples.

[0035] The technical effect of the above solution is that the bottom of cavity C is at the same height as the bottom of cavity D, which facilitates manufacturing and comparison of sample storage volume between different cavities.

[0036] The technical effects of the above-mentioned technical solution are: the multifunctional detection device can acquire magnified microscopic images; perform formed element analysis based on the images; and perform immune detection and quarantine analysis of the samples based on the optical reaction characteristics of the samples. Attached Figure Description

[0037] Figure 1 This is a schematic block diagram of a multi-functional detection device;

[0038] Figure 2 This is a schematic diagram of a multi-functional detection device;

[0039] Figure 3 This is a schematic diagram of a multifunctional sample container for testing;

[0040] Figure 4 This is a schematic diagram of a multifunctional sample container for testing;

[0041] Figure 5 This is a schematic diagram of the disassembled state of the multifunctional sample container device.

[0042] Figure 6 This is a schematic diagram of a multifunctional sample container for testing;

[0043] Figure 7 This is a schematic diagram of the disassembled state of the multifunctional sample container device. Detailed Implementation

[0044] The contents of this application will be further described in detail below with reference to the accompanying drawings.

[0045] It should be noted that the following description of preferred embodiments of this application does not constitute any limitation on this application. The description of preferred embodiments is merely an illustration of the general principles of this application. The embodiments described in this application are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0046] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the equipment or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. Furthermore, the terms "first," "second," and technical features numbered with Arabic numerals 1, 2, 3, etc., and designations such as "A" and "B," are used for descriptive purposes only, for ease of explanation, and do not represent a temporal or spatial order; they should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first," "second," and numbered with Arabic numerals 1, 2, 3, etc., may explicitly or implicitly include one or more of that feature. In the description of this application, "several" means two or more, unless otherwise expressly and specifically defined.

[0047] like Figures 1 to 3 In one embodiment of a multifunctional sample container, a detection unit A and a detection unit B are included. Detection unit A includes a cavity A, i.e., a formed element detection cavity. Cavity A is used to contain sample A and to capture a magnified microscopic image of the microscopic sample, which is used for formed element analysis. Detection unit B includes a cavity B, i.e., an optical detection cavity. Cavity B is used to contain sample B and to perform optical detection and analysis of the sample, which is used for immunoassay analysis of sample B.

[0048] like Figure 3 In one embodiment of a multifunctional sample receiving device, the detection unit B further includes a sample inlet B; the cavity B is connected to the sample inlet B; the detection unit A further includes a sample inlet A; the cavity A is connected to the sample inlet A.

[0049] like Figures 3 to 5In one embodiment of a multifunctional sample containing device, a reagent containing section C is further included. The reagent containing section C includes a cavity C and a sample dispensing port C, which are connected. The cavity C is used to contain detection reagents, and the sample dispensing port C faces upwards, through which liquid is added to or removed from the cavity C. The cavity C is used to contain staining reagents; or the cavity C is used to contain immunoassay reagents or biochemical assay reagents. The immunoassay reagents or biochemical assay reagents include detection microparticles, the surface of which includes antigens or antibodies. When the detection microparticles are added to sample B, they aggregate, causing changes in the optical properties of sample B. The cavity C can also be used for optical detection.

[0050] like Figures 3 to 5 In one embodiment of a multifunctional sample container, a sample preparation unit D is further included. The sample preparation unit D includes a cavity D and a sample dispensing port D, and the cavity D and the sample dispensing port D are connected. The cavity D is used for preparing sample A; or the cavity D is used for preparing sample B. There can be one or more cavities D. There can also be one or more cavities D. The cavity D can also be used for optical detection.

[0051] like Figure 5 In one embodiment of a multifunctional sample receiving device, cavities C and A are located on the same side of the main body of the device. The bottom of cavity C may be higher than the sample dispensing port A. Protective films are provided on the tops of cavities B, C, and D; these films may be provided individually or as a whole. An external device can puncture the protective film to enter sample dispensing port B and add the corresponding liquid to cavity B. An external device can puncture the protective film to enter sample dispensing port C and add the corresponding liquid to cavity C. An external device can puncture the protective film to enter sample dispensing port D and add the corresponding liquid to cavity D.

[0052] like Figure 6 and Figure 7 In one embodiment of a multifunctional sample receiving device, cavity C and cavity A are located on different sides of the main body of the device, facilitating the placement of various detection devices. The bottom of cavity C is lower than the sample dispensing port A; a protective membrane is provided at the bottom of cavity C for sealing. An external device can puncture the protective membrane to enter the sample dispensing port C and add the corresponding liquid to cavity C. An external device can puncture the protective membrane to enter the corresponding sample dispensing port and add the corresponding liquid to the corresponding cavity.

[0053] like Figure 6 and Figure 7 In one embodiment of a multifunctional sample container, the bottom of cavity C is at the same height as the bottom of cavity D.

[0054] like Figures 1 to 2An embodiment of a multifunctional detection device is provided for use in conjunction with a multifunctional sample containing device, including a microscopic magnification digital imaging component and an optical detection component; the microscopic magnification digital imaging component is used to capture magnified microscopic images of the microscopically examined sample; an image of the sample in cavity A is obtained for formed element analysis; light from the optical detection component illuminates the sample in cavity B of the external multifunctional detection device, and the sample is subjected to immunoassay analysis based on the optical reaction characteristics of the sample.

[0055] like Figures 1 to 2 An embodiment of a multifunctional detection device includes an optical detection component comprising a light source and a light detection component; the light source illuminates the cavity B, and the light detection component acquires the light passing through the cavity B to obtain optical reaction characteristic information of the sample.

[0056] While this application has been described and illustrated with reference to preferred embodiments and several alternatives, it is not intended to be limited to the specific descriptions herein. Other alternatives or equivalent components may also be used in practicing this application.

Claims

1.A multifunctional detection sample container, comprising: a detection part A and a detection part B; the detection part A comprises a cavity A, the cavity A is used for containing a sample A, the cavity A is used for taking a microscopic magnified image of a microscopic sample, and the microscopic magnified image is used for morphological component analysis; and the detection part B comprises a cavity B, the cavity B is used for containing a sample B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for immunoassay of the sample B. 2.The multifunctional detection sample container according to claim 1, wherein: the detection part B further comprises a sample adding port B, and the cavity B is communicated with the sample adding port B; and the detection part A further comprises a sample adding port A, and the cavity A is communicated with the sample adding port A. 3.The multifunctional detection sample container according to claim 2, further comprising a reagent container C, the reagent container C comprises a cavity C and a sample adding port C, the cavity C is communicated with the sample adding port C, the cavity C is used for containing a detection reagent, and the sample adding port C is upwardly open and used for adding or removing liquid to or from the cavity C. 4.The multifunctional detection sample container according to claim 3, wherein: the cavity C is used for containing a staining reagent; or the cavity C is used for containing an immunoassay reagent or a biochemical reagent. 5.The multifunctional detection sample container according to claim 4, wherein: the immunoassay reagent or the biochemical reagent comprises detection microparticles, a surface of the detection microparticle comprises an antigen or an antibody, when the detection microparticle is added into the sample B, the detection microparticle is aggregated, and the optical property of the sample B is changed. 6.The multifunctional detection sample container according to claim 3, further comprising a sample preparation part D, the sample preparation part D comprises a cavity D and a sample adding port D, the cavity D is communicated with the sample adding port D, the cavity D is used for preparation of the sample A; or the cavity D is used for preparation of the sample B. Any one of the following technical features is included: TA10: a bottom of the cavity C is higher than the sample adding port A; TA20: a bottom of the cavity C is lower than the sample adding port A; TA30: the detection part A and the detection part B are located on the same side of the multifunctional detection sample container; TA40: the detection part A and the detection part B are located on different sides of the multifunctional detection sample container; TA50: the cavity A is used for morphological component analysis, and the morphological component analysis comprises any one or more of morphological analysis, biochemical analysis and immunoassay analysis. The bottom of the cavity C is at the same height as the bottom of the cavity D. 9.A multifunctional detection device, used in cooperation with the multifunctional detection sample container according to any one of claims 1 to 8, comprising a microscopic magnified digital imaging assembly and an optical detection assembly; the microscopic magnified digital imaging assembly is used for taking a microscopic magnified image of a microscopic sample, and an image of a sample in the cavity A is obtained for morphological component analysis; and the optical detection assembly is used for irradiating a sample in the cavity B of the multifunctional detection device with light, and performing immunoassay of the sample according to an optical reaction characteristic of the sample. ​ 7. The multi-functional sample container of claim 3, wherein ​ ​ ​ ​ ​ ​ 8. The multi-functional sample container of claim 6, wherein ​ ​ ​ ​ ​ 10. The multi-functional detection device according to claim 9, wherein The optical detection assembly comprises a light source and a light detection assembly; the light source irradiates the cavity B, and the light detection assembly acquires light passing through the cavity B to obtain optical reaction characteristic information of the sample.

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