Culture and observation device for ground simulation weightlessness experiment

Abstract

The utility model discloses a culture and observation device for a ground simulation weightlessness experiment, which comprises a culture bottle body, a cell carrying unit and an organ chip carrying unit, a bottle opening of the culture bottle body is provided with a gas-liquid separation plug, the bottle bottom can be opened and closed and is provided with a sealing gasket, and the cell carrying unit can be used for conventional cell culture after being arranged in the culture bottle body. A simulated weightlessness experiment can be carried out on a ground cell gyroscope by changing the solution and injecting the perfusion culture solution, and a cell carrying unit taken out after the experiment can be directly dyed; alternatively, the organ chip mounting unit is replaced and mounted, and the organ chip unit is mounted. The culture and observation device disclosed by the utility model has the advantages of simple perfusion operation and high perfusion culture solution utilization rate, can be conveniently used for experimental observation of cell and organ chips in a ground simulation weightless environment, and can be repeatedly used after sterilization, so that the experimental efficiency is improved, and the experimental cost is reduced.

Description

Technical Field

[0001] This utility model relates to the field of cell culture and organ-on-a-chip technology, specifically to culture flasks for carrying and observing cell and organ-on-a-chip experiments to realize ground-based simulated weightlessness experiments. Background Technology

[0002] In recent years, aerospace medicine has developed rapidly. Cell culture in simulated weightlessness environments on Earth (e.g., using cell vortex culture systems) helps to understand the effects of microgravity on multiple tissue systems in organisms (e.g., the human body). T25 cell culture flasks are commonly used for cell culture in simulated weightlessness environments on Earth. However, conventional T25 cell culture flasks have a large volume. To prevent air bubbles in the liquid culture medium from shearing cells attached to one side of the flask during rotation and causing cell detachment, it is often necessary to add culture medium in multiple small amounts using a pipette until the flask is full. This perfusion method of removing air bubbles makes perfusion experiments more complex and increases the risk of contamination. More importantly, the utilization rate of perfusion culture medium is low, which is not conducive to controlling experimental costs. When using conventional T25 cell culture flasks, there are limitations in cell culture manipulation and a series of problems arising from this. Common problems include the need to digest the cells in the culture flask before transferring them to a confocal dish for immunofluorescence staining (for example, in CN205528844U, although a partition is set inside the culture flask and a gas exchange space is formed above the partition, the cells in the culture medium on and below the partition cannot be directly stained and still need to be digested and transferred first), which often leads to unsatisfactory experimental observation results.

[0003] Besides cell culture, organ-on-a-chip, as an advanced biotechnology tool widely used in drug development and testing, disease research, personalized medicine, toxicology research, and regenerative medicine (see CN113862154A, etc.), simulates the structure and function of different organs in organisms (such as the human body). It can be used in relevant experimental systems (such as cell cyclotron culture systems) to help researchers gain a deeper understanding of the effects of the microgravity environment in space. However, these experiments lack the corresponding structural units for mounting and observing organ-on-a-chip. Typically, organ-on-a-chip experiments must undergo complex experimental procedures and face significant contamination risks to simulate weightlessness on Earth, resulting in less than ideal experimental results.

[0004] In addition, CN105602846A proposed a cell culture experimental device with observation and chip-mounted structure. However, on the one hand, it is directly used for space experiments, and the perfusion flow rate inside the chip is already very low; on the other hand, the cultured cells are not suitable for staining observation (due to the low biomass of the cell culture, the staining results are not obvious, so a CCD component is used for microscopic image acquisition and analysis). Summary of the Invention

[0005] The purpose of this invention is to provide a culture and observation device for ground-based simulated weightlessness experiments, which can efficiently, conveniently, and at low cost carry out related experimental operations by mounting cell and organ chips.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A culture and observation device for terrestrial simulated weightlessness experiments includes a culture bottle body and a first cell mounting unit. The culture bottle body includes a bottle body and a bottle opening disposed on one side of the bottle body. The side of the bottle body opposite to the bottle opening (i.e., the bottom side of the bottle) adopts an openable and closable structure. The bottle body is also provided with a plurality of slot structures extending from its bottle opening side to the other side of the bottle body (i.e., the bottom side of the bottle). The first cell mounting unit includes a plate structure that can be embedded into the bottle body by cooperating with the corresponding slot structures (e.g., a set of U-shaped slots) and dividing the internal space of the bottle body (i.e., dividing the internal space of the bottle body into two).

[0008] Preferably, the culture and observation device further includes a second cell-carrying unit, which includes a plate structure that can fit tightly against the inner wall of the bottle and provide the same cell culture area as the plate structure of the first cell-carrying unit by cooperating with a corresponding slot structure (e.g., a set of L-shaped slots).

[0009] Preferably, the plate structure of the first cell carrier unit and the second cell carrier unit is made of materials suitable for cell culture, wherein the plate structure of the first cell carrier unit is made of polytetrafluoroethylene or glass, and the plate structure of the second cell carrier unit is made of polytetrafluoroethylene or glass.

[0010] Preferably, the culture and observation device further includes a gas-liquid separation plug, which includes a plug body disposed inside the mouth of the culture bottle body, and the plug body is provided with a ventilation channel and a filling channel respectively.

[0011] Preferably, the plug is made of a material that allows gas but not liquid to pass through (e.g., a membrane separation material used in a degassing membrane assembly) and has pre-drilled venting and injection channels, the diameter of which is adapted to the specifications of the syringe needle.

[0012] Preferably, the culture and observation device further includes a bottle cap, which is detachably mounted on the bottle opening.

[0013] Preferably, the culture and observation device further includes an organ-on-a-chip mounting unit and a fixing unit. The organ-on-a-chip mounting unit includes a plate structure that can be embedded into the bottle body by cooperating with a corresponding slot structure (such as a set of U-shaped slots) and converting the space inside the bottle body into space only for observing the supported organ-on-a-chip unit (containing organ tissue culture medium, etc.). The fixing unit includes fixing screws for connecting the organ-on-a-chip unit to the plate structure, thereby supporting the organ-on-a-chip unit in the space inside the bottle body.

[0014] Preferably, the plate structure and fixing unit of the organ-on-a-chip carrier unit are both made of polytetrafluoroethylene.

[0015] Preferably, the openable structure further includes a push-button switch disposed on the body of the culture bottle for controlling the opening and closing state of the side opposite to the bottle opening (i.e., the bottom side of the bottle), and a sealing gasket is disposed on the side opposite to the bottle opening (i.e., the bottom side of the bottle).

[0016] Preferably, the entire culture bottle body (including most of the bottle body and the bottle mouth) is made of polytetrafluoroethylene, and the bottle body is also provided with an observation area for observing the cells cultured on the first cell carrier unit (or the organ-on-a-chip unit fixed on the organ-on-a-chip carrier unit) directly through the bottle body, and the observation area is made of glass.

[0017] Preferably, the bottle body (specifically the outer wall of the bottle body) is also provided with a frosted area for easy marking.

[0018] Preferably, the culture flask body adopts the same specifications as a conventional T25 cell culture flask (e.g., cell culture area of ​​25 cm²). 2 ).

[0019] Preferably, the culture and observation device further includes a cell observation support unit and a cell separation unit for partitioning the cell carrier unit placed in the cell observation support unit (for example, after the perfusion experiment, the second cell carrier unit and the cells cultured on its plate structure can be removed and transferred to the cell observation support unit). The cell observation support unit includes a box-shaped structure composed of a support base plate and support side plates (for example, it is formed by connecting one support base plate with four support side plates located around the perimeter of the support base plate). One of the support side plates and the adjacent support side plates are sequentially (respectively) provided with notches and transverse slots for the cell separation units to be embedded and positioned on the support base plate (the two are aligned in height).

[0020] Preferably, the cell separation unit includes a cross-shaped plate structure, each end of which is inserted into a vertical slot (adjacent to a horizontal slot on the corresponding bearing side plate) provided on each bearing side plate.

[0021] The beneficial effects of this utility model are reflected in:

[0022] The culture and observation device of this invention can be used for ground-based simulated weightlessness experiments. Compared with conventional T25 cell culture flasks, by setting a slot structure inside the culture flask body and combining it with a cell carrier unit that fits into the corresponding slot structure, the utilization rate of perfusion culture medium can be improved (for example, when filled with the same perfusion culture medium, the culture needs of two layers of cells can be met simultaneously in the experiment), and the experimental cost can be reduced. At the same time, the cell carrier unit can be removed for direct staining, thereby improving the experimental effect.

[0023] Furthermore, the culture bottle body of this invention is provided with a gas-liquid separation plug at its mouth, which can significantly shorten the filling and bubble removal time and reduce the risk of contamination.

[0024] Furthermore, the organ-on-a-chip mounting unit of this invention can be used to replace the cell mounting unit and be installed inside the culture flask. Combined with the fixation unit, it can provide an effective way to mount organ-on-a-chip units of various specifications quickly, reliably, and with low risk of contamination in experiments.

[0025] Furthermore, the openable structure used in this invention (e.g., opening and closing via a push-button switch on the bottom side of the bottle) can be used to open and close the culture bottle body from the side, and with the cooperation of the sealing gasket, the cell-carrying unit or organ-on-a-chip-loaded unit placed inside the culture bottle body is in a state of good sealing and isolation from the outside world, effectively preventing leakage during experiments or contamination of the organ-on-a-chip unit due to exposure to the external environment.

[0026] Furthermore, the observation area on the culture flask body in this invention is made of glass (which refracts light differently than polytetrafluoroethylene), allowing direct visual observation of cells or organ-on-a-chip units or observation under a microscope without removing the cell-carrying unit or organ-on-a-chip unit. This simplifies experimental procedures and reduces the risk of contamination.

[0027] Furthermore, the culture flask body of this invention has a flat exterior (no different in shape from a conventional T25 cell culture flask), which can be directly adapted to a cell rotary instrument, making the culture and observation device of this invention more suitable for widespread application.

[0028] Furthermore, the cell observation carrier unit in this invention has horizontal and vertical slots on its inner wall, which can be used to embed the cell carrier unit and the cell segmentation unit. The cells cultured on the cell carrier unit can be divided into sections by the cell segmentation unit so that multiple antibodies can be used to incubate and stain them separately, thereby improving experimental efficiency.

[0029] Furthermore, the culture flask body, cell carrier unit, and other components in this invention are made of high-temperature resistant materials such as polytetrafluoroethylene and glass, which can be repeatedly sterilized and reused, thus helping to reduce experimental costs. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the culture flask structure after modification of the conventional T25 cell culture flask in Example 1;

[0031] Figure 2-1 This is an overall structural diagram of the cell culture and observation device in Example 2 (a cell carrier unit is shown in the diagram, but the push-button switch and sealing gasket are not shown).

[0032] Figure 2-2 yes Figure 2-1 A three-dimensional view of the cell-carrying unit shown in the image;

[0033] Figure 2-3 yes Figure 2-1 A cross-sectional view of the cell-carrying unit shown in the image;

[0034] Figure 2-4 This is a schematic diagram of the assembly of the cell observation carrier unit and the cell segmentation unit used to stain the removed cell carrier unit in Example 2 (the horizontal slot is not shown in the figure).

[0035] Figure 2-5 yes Figure 2-4 The three-dimensional view (a), top view (b), and cross-sectional view (c) of the cell observation carrier unit shown in the figure.

[0036] Figure 2-6 yes Figure 2-4 A three-dimensional view of the cell segmentation unit shown;

[0037] Figure 3-1 This is a general structural diagram of the organ-on-a-chip mounting and observation device in Example 3 (the organ-on-a-chip unit is installed in the figure, but the push-button switch, frosted area, bottle mouth, and bottle cap are not shown).

[0038] Figure 3-2 yes Figure 3-1 Top view (a) and cross-sectional view (b) of the organ-on-a-chip unit shown.

[0039] Figure 3-3 yes Figure 3-1 Top view (a) and cross-sectional view (b) of the organ-on-a-chip unit shown;

[0040] Figure 3-4 yes Figure 3-1 The front view (a) and top view (b) of the fixing unit (specifically the fixing screw) shown in the figure;

[0041] In the diagram: 1 is the culture flask body, 2 is the cell mounting unit, 3 is the cell observation carrying unit, 4 is the cell separation unit, 5 is the organ-on-a-chip mounting unit, 6 is the organ-on-a-chip unit, 7 is the fixing unit, 11 is the gas-liquid separation plug, 12 is the bottle cap, 13 is the push-button switch, 14 is the sealing gasket, 15 is the L-shaped slot, 16 is the frosted area, 17 is the U-shaped slot, 18 is the observation area, 21 is the cell mounting protrusion, 31 is the carrying base plate, 32 is the carrying side plate, 33 is the horizontal slot, 34 is the vertical slot, 51 is the organ-on-a-chip mounting protrusion, 61 is the through hole, 71 is the threaded hole, and 72 is the fixing screw. Detailed Implementation

[0042] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The embodiments described are only for explaining the present invention and are not intended to limit the scope of protection of the present invention.

[0043] Example 1

[0044] See Figure 1 To accommodate both cell culture and observation, as well as organ-on-a-chip (O2-A) experiments in simulated weightlessness on Earth, the conventional T25 cell culture flask was structurally modified. The modified flask consists of a cap 12 and a body. The cap 12 seals the opening on one side of the body, into which a gas-liquid separator 11 can be inserted. The side of the body opposite the opening (the bottom side) has an openable / closable structure. The bottom and the adjacent side of the body (this side of the body has a central observation area 18) are also included. Figure 1 The observation area 18 is located in the middle of the top surface of the bottle. A push-button switch 13 is located on one side of the bottom of the bottle. Figure 1 Specifically, the upper side of the bottle bottom) and the hinge (located on the other side of the bottle bottom, in Figure 1 Specifically, the bottom side of the bottle; the push-button switch 13 mainly consists of a plug and a socket. The plug is fixed to the bottom of the bottle, and the socket is fixed to the side of the bottle body adjacent to the bottom and can be plugged in (the specific plugging method can be referred to the switch of the car glasses case for corresponding structural design); the bottom of the bottle is also provided with a sealing gasket 14 made of heat-resistant silicone, and the sealing gasket 14 can cover the four edges of the bottom of the bottle, so that after the bottom of the bottle is closed by using the push-button switch 13, the bottom of the bottle can be tightly fitted to the sealing gasket 14 to keep the bottle body (specifically the bottom side of the bottle) in a sealed state; the other two sides of the bottle body adjacent to the bottom and opposite each other (one of which has a frosted area 16 on the outer surface to facilitate marking with a marker pen, in Figure 1The frosted area 16 is located on the side of the bottle closest to the paper surface. It has two sets of slot structures (each set is symmetrically arranged on the inner surface of both sides of the bottle). The set located in the middle of both sides of the bottle is a set of U-shaped slots 17 with opposite positions, while the set located on the edges of both sides of the bottle (i.e. near the corner of the bottle below the set of U-shaped slots 17) is a set of L-shaped slots 15 with opposite positions.

[0045] The gas-liquid separation plug 11 is made of membrane separation material from existing degassing membrane components (which can remove gas mixed in liquids) (specifically, it can be made of hollow fiber membrane filaments woven or stacked). It is cylindrical in shape and can be tightly embedded into the bottle mouth along its axis to form a plug body. The plug body is provided with ventilation channels and filling channels that can connect the inside and outside (specifically, the inside of the bottle and the external environment). When injecting the perfusion culture medium, simultaneously insert syringe needles into both channels. The perfusion culture medium is injected into the bottle through the syringe and the syringe needle at the perfusion channel (structural units for cell attachment can be pre-loaded through the U-shaped slot 17 and / or L-shaped slot 15). When the bottle is almost full (or after injecting a certain amount of perfusion culture medium), use another empty syringe to aspirate through the syringe needle at the vent channel to release air bubbles mixed in with the perfusion culture medium inside the bottle. After the air bubbles have risen completely, fill the bottle with perfusion culture medium. At this time, the residual gas inside the bottle will permeate through the micropores of the stopper itself, ensuring that the liquid injected into the culture bottle is free of air bubbles. Then, screw the bottle cap 12 onto the bottle mouth, tighten the bottle cap 12, and place it on the cell rotation instrument to complete the perfusion experiment under the simulated weightlessness environment on the ground.

[0046] Example 2

[0047] See Figure 2-1 This embodiment provides a cell culture and observation device for ground-based simulated weightlessness experiments. The device includes a culture flask body 1 and a cell-carrying unit 2. The culture flask body 1 is a modified culture flask as described in Embodiment 1, and the cell-carrying unit 2 is inserted into the flask body from the bottom side (e.g., via an openable / closable bottom). Figure 2-1 The cell carrier unit 2 is secured in a slot structure near the lower corner of the vial body and is tightly pressed against the bottom of the vial. For details of this slot structure, please refer to [link / reference needed]. Figure 1The L-shaped slot 15 in the culture flask allows for routine cell culture and medium changes after the cell carrier unit 2 is installed. When perfusion is needed, the cell culture medium can be injected through the flask opening (specifically via the gas-liquid separator plug 11) using a syringe or peristaltic pump (e.g., LongerPump BT100-1L). (After filling, the flask opening is resealed with the cap). This avoids the need for multiple small injections using a pipette, simplifying the operation and significantly reducing the risk of contamination. The culture flask body 1 is primarily made of polytetrafluoroethylene (with a portion of the outer surface made of glass for easy observation; the glass observation area is 0.17mm ± 0.02mm thick), and the culture area is 25cm². 2 Not only is it highly compatible with cell gyroscopes, allowing direct mounting, but it also allows for simultaneous placement of two cell-carrying units 2 at different heights below the glass observation area inside the vial (i.e., in...). Figure 2-1 When a cell carrier unit 2 is inserted into the middle half of the bottle via a slot structure, the details of which can be found in [reference needed]. Figure 1 The U-shaped slot 17 in the bottle, filled with bubble-free cell culture medium, can simultaneously supply the culture needs of the cells attached to the two cell carrier units 2, thereby improving the utilization rate of cell culture medium (i.e., perfusion culture medium) in the perfusion experiment. Furthermore, to avoid the lower cell carrier unit 2 potentially affecting the observation of the upper cell carrier unit 2 when two cell carrier units 2 are loaded simultaneously for the experiment, only one cell carrier unit 2 can be loaded in the middle of the bottle, and the cells attached to the cell carrier unit 2 located in the middle of the bottle can be directly observed through the observation area during the experiment.

[0048] See Figure 2-2 and Figure 2-3 The cell-carrying unit 2 is a double-layer plate structure (made of polytetrafluoroethylene or glass; glass cell-carrying units can be used for cell immunofluorescence staining, while polytetrafluoroethylene cell-carrying units are used for cell protein and mRNA extraction experiments, as well as other staining experiments that do not have strict observation requirements). The area of ​​the two plates is smaller at the top and larger at the bottom, thus forming cell-carrying protrusions 21 on both sides (belonging to the lower plate of the cell-carrying unit 2). These protrusions are used for the cell-carrying unit 2 to be embedded in different slot structures on the inner surface of the bottle. The upper plate surface of the cell-carrying unit 2 is used for inoculating and attaching the cells required for the experiment. The corresponding cell inoculation information is marked on the outer surface of the bottle so as to distinguish the cells carried in different culture bottles during perfusion experiments.

[0049] See Figure 2-4 and Figure 2-5The cell culture and observation device further includes a cell observation support unit 3 and a cell separation unit 4. The cell observation support unit 3 includes a support base plate 31 (made of glass with a thickness of 0.17mm±0.02mm) and support side plates 32 (made of polytetrafluoroethylene) arranged around the support base plate 31. The lower part of the inner wall of the two opposing support side plates 32 is provided with a horizontal slot 33 (also L-shaped), which allows the cell carrier unit 2 to be inserted into the support base plate 31 from the lower notch of another support side plate 32 adjacent to the two support side plates 32. The middle part of the inner wall of the four support side plates 32 is provided with a vertical slot 34, which allows the cell separation unit 4 to be inserted.

[0050] See Figure 2-6 The cell separation unit 4 adopts a cross-shaped structure composed of two plates (made of polytetrafluoroethylene). The lower surface of the structure is flush, and the end can be placed on the cell carrier unit 2, which has been embedded in the cell observation carrier unit 3 through the horizontal slot 33, via the vertical slot 34. This divides the area on the cell carrier unit 2 with attached cells into four parts. After the ground cell simulated weightlessness experiment (e.g., perfusion experiment), one or two cell carrier units 2 can be taken out of the culture flask for subsequent experimental operations (e.g., the taken-out cell carrier unit 2 is placed on the corresponding cell observation carrier unit 3 for partitioning and then immunofluorescence staining by incubating different antibodies), and then the cells can be observed under an optical microscope or a fluorescence microscope.

[0051] Example 3

[0052] See Figure 3-1 This embodiment provides an organ-on-a-chip mounting and observation device for ground-based simulated weightlessness experiments. The device includes a culture flask body 1, an organ-on-a-chip mounting unit 5, and a fixing unit 7. The culture flask body 1 is a modified culture flask as described in Embodiment 1, and its interior provides space for mounting and observing the organ-on-a-chip. The organ-on-a-chip mounting unit 5 and the organ-on-a-chip unit 6 fixed thereon are inserted into the flask body from the openable bottom side (e.g., ...). Figure 3-1 The organ-on-a-chip carrier unit 5 is secured in a slot structure located at the midpoint of the bottle's height, and is tightly pressed against the bottom of the bottle. For details of this slot structure, please refer to [link / reference needed]. Figure 1 After inserting the U-shaped slot 17, the sealed culture flask body 1 can be directly placed on the flask opening. The cap remains tightly screwed on before and after inserting the organ-on-a-chip unit 5 and organ-on-a-chip unit 6. No perfusion is required during organ-on-a-chip insertion, as the organ-on-a-chip unit 6 already contains culture medium. No cell culture is performed during organ-on-a-chip insertion; that is, the slot structure near the lower corner of the flask body is empty and the cell-on-a-chip unit 2 is not inserted. For details of this slot structure, please refer to [link to relevant documentation]. Figure 1The L-shaped slot 15 is fixed to the cell gyroscope for rotation. The main material of the culture flask body 1 is polytetrafluoroethylene (partial areas of the outer surface of the flask body are made of glass to facilitate observation during rotation, and the thickness of the glass observation area is 0.17mm±0.02mm).

[0053] See Figure 3-2 The organ-on-a-chip mounting unit 5 has a three-layer plate structure (made of polytetrafluoroethylene), with the middle plate having the largest area (larger than the two plates above and below it with the same area). This creates organ-on-a-chip mounting protrusions 51 on both sides, which are used to allow the organ-on-a-chip mounting unit 5 to be embedded in a slot structure at a specific position on the inner surface of the bottle. The uppermost plate of the organ-on-a-chip mounting unit 5 is used to place the organ-on-a-chip unit 6. The fixing unit 7 includes threaded holes 71 arranged symmetrically on both sides of the organ-on-a-chip mounting unit 5 according to different organ-on-a-chip unit 6 specifications. There can be four threaded holes 71, each penetrating the three-layer plate structure of the organ-on-a-chip mounting unit 5. By selecting an organ-on-a-chip mounting unit 5 with threaded holes 71 in appropriate positions according to the specifications of the organ-on-a-chip unit 6, the corresponding organ-on-a-chip unit 6 can be fixed onto the organ-on-a-chip mounting unit 5.

[0054] See Figure 3-3 and Figure 3-4 The fixing unit also includes fixing screws 72 (made of polytetrafluoroethylene). Each organ-on-a-chip unit 6 has a through hole 61 opposite to the threaded hole 71. The fixing screws 72 are screwed into the threaded hole 71 through the hole 61 to fix the organ-on-a-chip unit 6, thus preventing oscillation of the organ-on-a-chip unit 6 during rotation. After a ground-based organ-on-a-chip simulation of weightlessness (e.g., after rotation), the culture flask body 1 containing the organ-on-a-chip unit 6 can be placed on the support of an inverted microscope and aligned with the observation area, allowing observation of the corresponding organ-on-a-chip state under the inverted microscope.

Claims

1. A culture and observation device for ground-based simulated weightlessness experiments, characterized in that: The culture and observation device comprises a culture bottle body (1) and a first cell carrying unit, the culture bottle body (1) comprises a bottle body and a bottle mouth, the side of the bottle body opposite to the bottle mouth is provided with an openable and closable structure, the bottle body is provided with a plurality of clamping groove structures extending from the bottle mouth side to the other side, and the first cell carrying unit comprises a plate body structure capable of being embedded into the bottle body by cooperating with the clamping groove structures and separating the space in the bottle body.

2. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The culture and observation device further comprises a second cell carrying unit, the second cell carrying unit comprises a plate body structure capable of closely adhering to the bottle body by cooperating with the clamping groove structures and providing the same culture area as the plate body structure of the first cell carrying unit.

3. The culture and observation device for ground-based simulated weightlessness experiments according to claim 2, characterized in that: The first cell carrying unit is made of polytetrafluoroethylene or glass, and the second cell carrying unit is made of polytetrafluoroethylene or glass.

4. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The culture and observation device further comprises a gas-liquid separation plug (11), the gas-liquid separation plug (11) comprises a plug body arranged in the bottle mouth, and the plug body is respectively provided with a gas passage and a perfusion passage; the bottle mouth is provided with a detachable bottle cap (12).

5. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The culture and observation device further comprises an organ chip carrying unit (5) and a fixing unit (7), the organ chip carrying unit (5) comprises a plate body structure capable of being embedded into the bottle body by cooperating with the clamping groove structures and converting the space in the bottle body into an observation area for the organ chip unit (6), and the fixing unit (7) comprises a fixing screw (72) for connecting the organ chip unit (6) to the plate body structure.

6. The culture and observation device for ground-based simulated weightlessness experiments according to claim 5, characterized in that: The organ chip carrying unit (5) and the fixing unit (7) are both made of polytetrafluoroethylene.

7. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The side of the bottle body opposite to the bottle mouth is provided with a sealing gasket (14).

8. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The bottle body is further provided with an observation area (18) made of glass.

9. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The bottle body is further provided with a frosted area (16).

10. The culture and observation device for ground-based simulated weightlessness experiments according to claim 1, characterized in that: The culture area of the culture bottle body (1) is 25cm 2 .

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