Gene sequencing optical system and gene sequencer
By using a dichroic mirror array to separate imaging light in a gene sequencer, the use of fluorescent dyes is reduced, solving the problems of complex and high cost in optical system design, and achieving cost reduction and design simplification.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-27
- Publication Date
- 2026-03-13
AI Technical Summary
The design of existing gene sequencer optical systems is difficult and costly, mainly due to the need for a variety of fluorescent dyes and complex optical component designs.
A dichroic mirror group is used to separate the imaging light generated after the base sample is excited by excitation light into three fluorescence bands of three base types, reducing the use of one fluorescent dye and simplifying the design of the optical system.
It significantly reduces the cost of gene sequencing optical systems and fluorescent dye reagents, simplifies design requirements, and maintains the same sequencing efficiency.
Smart Images
Figure CN223993017U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of gene sequencing technology, and in particular to a gene sequencing optical system and a gene sequencer. Background Technology
[0002] Microscopic imaging systems are widely used in gene sequencers. When a gene sequencer is working, it is necessary to perform fluorescence imaging of the four bases A, T, G, and C to determine the base sequence in DNA.
[0003] To achieve this, gene sequencers require an optical system, including an excitation module and a fluorescence imaging module. The excitation module induces stimulated emission of fluorescence from the four bases, while the imaging module images the fluorescence emitted by the bases. On the other hand, chemical methods are needed to attach different fluorescent groups to the A, T, G, and C bases, allowing them to emit fluorescence at different wavelengths upon excitation for differentiation. Currently known technologies modify the A, T, G, and C bases with different fluorescent groups, each emitting fluorescence in different wavelengths. To differentiate the four fluorescence bands emitted from a single imaging location, multi-channel imaging is commonly used, typically a dual-channel or four-channel system. This involves using a dichroic mirror to disperse the fluorescence across multiple wavelengths, with each channel including an imaging component. Filters are used to purify the fluorescence at each wavelength, resulting in four images of different bases. However, designing such an optical system for a gene sequencer is complex and costly. Summary of the Invention
[0004] To address the existing technical problems, this application provides a gene sequencing optical system and gene sequencer with lower cost and simplified design requirements.
[0005] In a first aspect, embodiments of this application provide a gene sequencing optical system, comprising:
[0006] A light source, used to emit excitation light of different wavelengths;
[0007] A dichroic mirror assembly includes a first dichroic mirror disposed in the outgoing light path of the light source and a second dichroic mirror disposed in the transmission light path of the first dichroic mirror. The first dichroic mirror reflects the excitation light emitted by the light source toward the base sample to be tested, and allows the imaging light generated by the base sample to be tested after being excited by the excitation light to pass through. The second dichroic mirror separates the imaging light into multiple channels of imaging light. The wavelength range of the light transmitted by the first dichroic mirror covers the fluorescence bands of the three base types, and the channel imaging light is the imaging light corresponding to the fluorescence bands of the three base types respectively.
[0008] The system includes multiple imaging channels, each of which includes an imaging module for forming a base image based on the imaging light of the corresponding channel.
[0009] In a second aspect, a gene sequencer is provided, including a displacement stage and the gene sequencing optical system described in any embodiment of this application;
[0010] The displacement stage is used to support the sequencing chip and move the sequencing chip so that the gene sequencing optical system can image the base sample to be tested at different positions on the sequencing chip.
[0011] The gene sequencing optical system provided in the above embodiments, with its dichroic mirror group, can separate the imaging light generated and reflected after the base sample to be tested is excited by excitation light, and obtain imaging light corresponding to the fluorescence bands of the three base types respectively. The entire gene sequencing optical system can reduce the use of one type of fluorescent dye, thereby reducing the detection requirement for one type of fluorescence band, and correspondingly reducing the design and manufacturing of optical elements for the fluorescence band corresponding to that fluorescent dye, thus simplifying design requirements and significantly reducing the cost of the gene sequencing optical system and the cost of fluorescent dye reagents.
[0012] The gene sequencer provided in the above embodiments belongs to the same concept as the corresponding gene sequencing optical system embodiments, and thus has the same technical effect as the corresponding gene sequencing optical system embodiments, which will not be repeated here. Attached Figure Description
[0013] Figure 1 This is a schematic diagram of the structure of a gene sequencing optical system in one embodiment.
[0014] Figure 2 This is a schematic diagram showing the fluorescence bands corresponding to the four base types.
[0015] Figure 3 A schematic diagram of a gene sequencing optical system including a dual-channel imaging system in one embodiment.
[0016] Figure 4 This is a schematic diagram of a gene sequencing optical system including a three-channel imaging system in one embodiment. Detailed Implementation
[0017] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
[0018] To make the objectives, technical solutions, and advantages of this application clearer, the application will be further described in detail below with reference to the accompanying drawings. The described embodiments should not be regarded as limitations on this application. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0019] In the following description, the phrase "some embodiments" refers to a subset of all possible embodiments. It should be noted that "some embodiments" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.
[0020] In the following description, the terms "first, second, and third" are used merely to distinguish similar objects and do not represent a specific ordering of objects. It is understood that "first, second, and third" may be interchanged in a specific order or sequence where permitted, so that the embodiments of this application described herein can be implemented in an order other than that illustrated or described herein.
[0021] To address the issues of high design difficulty and cost of current gene sequencer optical systems, the inventors of this application have conducted the following analysis of known gene sequencing optical systems:
[0022] For dual-channel imaging systems in gene sequencing optical systems, two excitation wavelengths need to be alternately activated sequentially. For each base sample to be tested at each location on the sequencing chip, the camera in each imaging channel takes an image once under excitation light of a different wavelength, thus obtaining images of the four bases A, T, G, and C respectively. On the one hand, due to the limitations of the camera itself, a data transmission time of tens of milliseconds is required after each exposure. However, each camera in a dual-channel imaging system needs to expose twice at the same location on the sample. Adding the time for the two data transmissions, this inevitably prolongs the imaging time. Each imaging channel needs to capture images of two bases; therefore, the filter for each channel needs to transmit two fluorescence bands. Only dual-bandpass filters can meet the requirements. The design difficulty and manufacturing cost of dual-bandpass filters are generally much higher than those of single-bandpass filters.
[0023] For a four-channel imaging system in gene sequencing optical systems, two excitation wavelengths can be turned on simultaneously. For each location on the sequencing chip, the camera in each imaging channel takes one picture, thus obtaining images of the four bases A, T, G, and C respectively. Compared to a two-channel imaging system, the four-channel imaging system can save at least half the imaging time. However, the imaging components in each imaging channel are more expensive, inevitably leading to an increase in cost.
[0024] In addition, fluorescent dyes with a single base type are also more expensive.
[0025] Based on this, the inventors of this application propose a gene sequencing optical system that uses fluorescence bands excited by three base types for base type identification. This reduces the use of one fluorescent dye and eliminates the need to design and manufacture optical components for the fluorescence bands generated by that dye, thus significantly reducing the cost of the gene sequencing optical system and the cost of the fluorescent dye reagents. Furthermore, based on the four base types (A, T, G, C) to be detected, the use of fluorescent dyes corresponding to the C base type (with the longest fluorescence band) is reduced. By reducing the detection requirements for the C base type, the overall spectral range that the gene sequencing optical system needs to support can be reduced, thereby lowering the design requirements for the main optical components required in the gene sequencing optical system.
[0026] Please see Figure 1 This is a schematic diagram of a gene sequencing optical system provided in an embodiment of this application, including: a light source 10 for emitting excitation light of different wavelengths; a dichroic mirror group 20, including a first dichroic mirror 21 disposed on the outgoing light path of the light source 10 and a second dichroic mirror 22 disposed on the transmission light path of the first dichroic mirror 21, the first dichroic mirror 21 is used to reflect the excitation light emitted by the light source 10 to the base sample 50 to be tested, and to allow the imaging light generated after the base sample 50 to be tested is excited by the excitation light to pass through; the second dichroic mirror 22 separates the imaging light generated after the base sample 50 to be tested is excited by the excitation light to form a multi-channel imaging light; wherein, the wavelength range of the light that the first dichroic mirror 21 can transmit covers the fluorescence bands of the three base types, and the channel imaging light is the imaging light corresponding to the fluorescence bands of the three base types respectively; a multi-channel imaging channel 30, each imaging channel 30 including an imaging module 306, the imaging module 306 being used to form a base image according to the imaging light of the corresponding channel.
[0027] Excitation light is light used to excite other substances (such as fluorescent substances) to produce light emission. Excitation light can be common types of light sources, such as thermal radiation sources (such as incandescent lamps), gas discharge sources (such as mercury lamps), or semiconductor sources (such as light-emitting diodes).
[0028] A dichroic mirror is a special optical element that selectively reflects or transmits light according to its wavelength. Dichroic mirrors operate based on the principle of multilayer thin-film interference, typically consisting of alternating layers of thin-film materials with different refractive indices. The thickness and material selection of these films are precisely designed to achieve specific spectral characteristics.
[0029] In the above embodiments, in the gene sequencing optical system, the dichroic mirror group 20 can separate the imaging light generated after the base sample 50 is excited by the excitation light, and obtain the imaging light corresponding to the fluorescence bands of the three base types. The entire gene sequencing optical system can reduce the use of one type of fluorescent dye, thereby reducing the detection requirement for one type of fluorescence band, and correspondingly reducing the design and manufacturing of optical elements for the fluorescence band corresponding to that fluorescent dye, thereby simplifying the design requirements and significantly reducing the cost of the gene sequencing optical system and the cost of fluorescent dye reagents.
[0030] In some embodiments, the base samples 50 to be tested in the gene sequencing optical system include A, T, G, and C bases. For the four base types A, T, G, and C, the use of fluorescent dyes for C bases is reduced. When the base sample 50 is excited by excitation light, A, T, and G bases emit A-base fluorescence, T-base fluorescence, and G-base fluorescence, respectively. Correspondingly, the wavelength range of light transmitted by the first dichroic mirror 21 covers the fluorescence bands of A, T, and G bases.
[0031] Please see Figure 2 The four fluorescence bands correspond to the four base types: A base (546–581 nm), T base (587–629 nm), G base (654–706 nm), and C base (712–794 nm). During gene sequencing imaging, it is necessary to ensure that the image quality across the entire field of view for all four base types reaches the diffraction limit, and that the color difference between the fluorescence bands for each base type is kept within a controllable range. The C base fluorescence band is the longest among the four base types. The inventors of this application have found that a wider fluorescence band places higher demands on the design of the optical system. Most commercially available optical elements suitable for the visible light band only support a maximum wavelength up to 750 nm, while the upper limit of the C base fluorescence band is close to 800 nm, entering the near-infrared band, which greatly limits the selection of optical elements. If optical components are independently developed, and image quality design requirements for near-infrared bands are added to the visible light band, the design difficulty will increase significantly, and the processing difficulty and cost will also increase accordingly. In this way, for the four base types A, T, G, and C, the use of fluorescent dyes of the C base type with the longest fluorescence band can be reduced accordingly. The design of the optical system can support a narrower spectral range, only within the visible light band of 546 to 706 nm, which greatly reduces the difficulty of selecting optical components or design requirements.
[0032] In some embodiments, please refer to Figure 3This is a schematic diagram of a gene sequencing optical system in a dual-channel imaging system according to one embodiment. An objective lens 40 is also provided between the first dichroic mirror 21 and the sample 50 to be tested. The wavelength range of light collected and transmitted by the objective lens 40 covers the fluorescence bands of A, T, and G bases. As an optical element in the gene sequencing optical system, the objective lens 40 needs to be compatible with imaging light of all bands generated by the excitation light so that imaging light of all bands can be reflected to the imaging system. This greatly limits the selection of the objective lens 40. In this embodiment, for the four base types A, T, G, and C, the use of C base type fluorescent dye is reduced, thereby reducing the detection requirement of the fluorescence band of the largest wavelength C base type. The spectral range that the objective lens 40 needs to reflect can be limited to the visible light band of 546-706nm, thereby reducing the difficulty of selecting or designing the objective lens 40.
[0033] Optionally, the imaging channel 30 includes a first imaging channel 31 and a second imaging channel 32. The wavelength range of light reflected by the second dichroic mirror 22 covers the fluorescence bands of A and G bases, and the wavelength range of light transmitted covers the fluorescence band of T bases. The first imaging channel 31 is located in the reflected light path of the second dichroic mirror 22, and the second imaging channel 32 is located in the transmitted light path of the second dichroic mirror 22. In this embodiment, the second dichroic mirror 22 works in conjunction with the first dichroic mirror 21. The first dichroic mirror 21 can reflect excitation light and transmit fluorescence bands of A, T, and G bases. The second dichroic mirror 22 can reflect fluorescence bands of A and G bases and transmit fluorescence bands of T bases. By using the second dichroic mirror 22 to form two separate imaging channels, the imaging light of the fluorescence bands corresponding to the three base types A, T, and G is separated by combining the illumination of excitation light of two wavelengths.
[0034] Optionally, the excitation light includes a first excitation light and a second excitation light with different wavelengths. The base sample 50 to be tested is excited by the first excitation light, emitting A-base fluorescence and T-base fluorescence; the base sample 50 to be tested is excited by the second excitation light, emitting G-base fluorescence. In the gene sequencing process, for the base sample 50 to be tested at the same position on the sequencing chip, the first excitation light and the second excitation light are alternately turned on once. When the first excitation light is turned on, the first imaging channel 31 and the second imaging channel 32 are each exposed once; when the second excitation light is turned on, the first imaging channel 31 is exposed once. Thus, three base images of the base sample 50 to be tested at the same position on the sequencing chip are obtained. Afterward, the sequencing chip moves to the next position, and the base sample 50 to be tested at the next position on the sequencing chip is photographed. Gene sequencing base clusters typically exceed a certain number, such as 50 or more. Consequently, more than 50 sequencing rounds are required. When analyzing the images, the positions of all base clusters can be located using images of the A, T, and G bases from at least 50 rounds. Based on the positions of the base clusters on the sequencing chip, bases with no signal at their corresponding A, T, and G cluster positions can be identified as C bases.
[0035] Optionally, the first imaging channel 31 further includes a first sleeve lens 311 and a first filter 312 disposed on the reflected light path of the second dichroic mirror 22. The first sleeve lens 311 is used to converge light, and the first filter 312 is a dual-bandpass filter that only allows light in the fluorescence bands of A and G bases to pass through. The corresponding imaging module 306 in the first imaging channel 31 is used to receive the converged light. The second imaging channel 32 further includes a second sleeve lens 321 and a second filter 322 disposed on the transmitted light path of the second dichroic mirror 22. The second sleeve lens 321 is used to converge light, and the second filter 322 is a single-bandpass filter that only allows light in the fluorescence band of T bases to pass through. The corresponding imaging module 306 in the second imaging channel 32 is used to receive the converged light.
[0036] In some embodiments, please refer to Figure 4This is a schematic diagram of a gene sequencing optical system of a three-channel imaging system in one embodiment. The dichroic mirror group 20 also includes a third dichroic mirror 23. The third dichroic mirror 23 is disposed on the outgoing light path of the transmitted light of the second dichroic mirror 22. The third dichroic mirror 23 separates the imaging light transmitted through the second dichroic mirror 22 to form multi-channel imaging light. The difference between this and the aforementioned dual-channel imaging system for gene sequencing optical systems is that a third dichroic mirror 23 is further provided in the dichroic mirror group 20. The imaging light of the fluorescence bands of the T base and G base transmitted by the second dichroic mirror 22 is separated by the third dichroic mirror 23. Thus, in the gene sequencing process, for the base sample 50 to be tested at the same position on the sequencing chip, the first excitation light and the second excitation light can be turned on simultaneously. The three base types of the base sample 50, namely A base, T base and G base, are excited by the excitation light and simultaneously generate light (i.e., light up). Through the cooperation of the first dichroic mirror 21, the second dichroic mirror 22 and the third dichroic mirror 23, the fluorescence bands of the corresponding three base types, namely A base, T base and G base, are separated to form a three-channel imaging light.
[0037] Correspondingly, the imaging channel 30 includes a first imaging channel 31, a second imaging channel 32, and a third imaging channel 33; the wavelength range of light reflected by the second dichroic mirror 22 covers the fluorescence band of A bases, and the wavelength range of light transmitted covers the fluorescence bands of T and G bases; the wavelength range of light reflected by the third dichroic mirror 23 covers the fluorescence band of G bases, and the wavelength range of light transmitted covers the fluorescence band of T bases; the first imaging channel 31 is located on the reflected light path of the second dichroic mirror 22, the second imaging channel 32 is located on the transmitted light path of the third dichroic mirror 23, and the third imaging channel 33 is located on the reflected light path of the third dichroic mirror 23.
[0038] In some embodiments, the first imaging channel 31 further includes a first sleeve lens 311 and a first filter 312 disposed on the reflected light path of the second dichroic mirror 22. The first sleeve lens 311 and the first filter 312 are sequentially arranged between the second dichroic mirror 22 and the imaging module 306. The first sleeve lens 311 is used to converge light, and the first filter 312 is a single bandpass filter that only allows light in the fluorescence band of the A base to pass through. The corresponding imaging module 306 in the first imaging channel 31 is used to receive the converged light. The second imaging channel 32 further includes a second sleeve lens 32 disposed on the transmitted light path of the second dichroic mirror 22. The second imaging channel 32 includes a second lens 321 for converging light, and a second lens 322 for converging light. The second lens 322 is a single bandpass filter that only allows light in the fluorescence band of the T base to pass through. The corresponding imaging module 306 in the second imaging channel 32 is used to receive the converged light. The third imaging channel 33 also includes a third lens 331 and a third lens 332 disposed on the reflected light path of the second dichroic mirror 22. The third lens 331 is used for converging light, and the third lens 332 is a single bandpass filter that only allows light in the fluorescence band of the G base to pass through. The corresponding imaging module 306 in the third imaging channel 33 is used to receive the converged light. In the gene sequencing process, for the base sample 50 to be tested at the same position on the sequencing chip, the first excitation light and the second excitation light can be turned on simultaneously. The three base types A, T and G in the base sample 50 are excited by the excitation light and lit up simultaneously. The imaging light of different fluorescence bands is separated by the dichroic mirror group 20 to form three channels of imaging light. The first imaging channel 31, the second imaging channel 32 and the third imaging channel 33 correspond to the three channels of imaging light, and each is exposed once, thereby obtaining three base images of the base sample 50 to be tested at the same position on the sequencing chip. After that, the sequencing chip moves to the next position and the base sample 50 to be tested at the next position on the sequencing chip is photographed.
[0039] The gene sequencing optical system provided in this application embodiment has at least the following characteristics:
[0040] First, it reduces the preparation cost of one fluorescent dye reagent, and the optical system correspondingly reduces the number of base images captured by one base type, thus solving a quarter of the image storage space issue.
[0041] Secondly, the preparation of fluorescent dyes corresponding to C-base types is reduced, consequently decreasing the optical system's detection requirements for the C-base type fluorescence band. To ensure the chromatic aberration of the optical system remains within a controllable range, a wider spectral range of fluorescence bands places higher demands on the design of optical components, such as the objective lens and the telescope lens. The C-base fluorescence band is the longest of the four base fluorescence bands, and the long, wide bands at the edges increase the design complexity of the optical system. By reducing the detection requirements for the C-base fluorescence band, the optical system needs to support a narrower spectral range, effectively reducing the difficulty of selecting or designing the optical system.
[0042] Third, for the imaging channel 30 in the optical system, the design of the dual bandpass filter in the imaging channel 30 can be simplified. For example, for a dual-channel imaging system, only one imaging channel 30 needs to be designed with a dual bandpass filter, while the other imaging channel 30 can use a single bandpass filter, thereby reducing the design difficulty and cost of optical components. For a three-channel imaging system, all three imaging channels 30 can use single bandpass filters. Compared with an optical system that can complete the acquisition of four base types of base images with only one exposure, the design and manufacturing cost of one imaging channel 30 is reduced while maintaining the same sequencing efficiency.
[0043] In another aspect, this application provides a gene sequencer, including a displacement stage 60 and a gene sequencing optical system according to any embodiment of this application; wherein, the displacement stage 60 is used to carry a sequencing chip and move the sequencing chip so that the gene sequencing optical system can image the base sample 50 to be tested at different positions on the sequencing chip.
[0044] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.
[0045] The above are merely specific embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
Claims
1. A gene sequencing optical system, characterized by, The method comprises the following steps: a light source for emitting excitation light of different wavelengths; a dichroic mirror set comprising a first dichroic mirror arranged on an outgoing light path of the light source and a second dichroic mirror arranged on a transmission light path of the first dichroic mirror, the first dichroic mirror being used for reflecting the excitation light emitted by the light source to a to-be-tested base sample and transmitting imaging light generated by the to-be-tested base sample after being excited by the excitation light; the second dichroic mirror separates the imaging light to form multi-channel imaging light; wherein the wavelength range of the light that can be transmitted by the first dichroic mirror covers the fluorescence wave bands of three types of bases, and the channel imaging light is the imaging light corresponding to the fluorescence wave bands of the three types of bases respectively; a plurality of imaging channels, each of the imaging channels comprising a shooting module, the shooting module being used for forming a base image according to the channel imaging light.
2. The genetic sequencing optical system of claim 1, wherein, The to-be-tested base types include A base, T base, G base and C base, and the to-be-tested base sample emits A base fluorescence, T base fluorescence and G base fluorescence after being excited by the excitation light. The wavelength range of the light that can be transmitted by the first dichroic mirror covers the fluorescence wave bands of A base, T base and G base.
3. The genetic sequencing optical system of claim 2, wherein, An objective lens is further arranged between the first dichroic mirror and the to-be-tested base sample; The wavelength range of the light that can be collected and transmitted by the objective lens covers the fluorescence wave bands of A base, T base and G base.
4. The genetic sequencing optical system of claim 2, wherein, The imaging channels include a first imaging channel and a second imaging channel; The wavelength range of the light that can be reflected by the second dichroic mirror covers the fluorescence wave bands of A base and G base, and the wavelength range of the light that can be transmitted covers the fluorescence wave band of T base, the first imaging channel is located on a reflection light path of the second dichroic mirror, and the second imaging channel is located on a transmission light path of the second dichroic mirror.
5. The genetic sequencing optical system of claim 4, wherein, The excitation light includes first excitation light and second excitation light of different wavelengths; The to-be-tested base sample emits A base fluorescence and T base fluorescence after being excited by the first excitation light, and emits G base fluorescence after being excited by the second excitation light.
6. The genetic sequencing optical system of claim 5, wherein, The first imaging channel further comprises a first sleeve lens and a first filter arranged on the reflection light path of the second dichroic mirror, the first sleeve lens is used for converging light, the first filter is a double-band pass filter, and only allows light of the fluorescence wave bands of A base and G base to pass through, and the shooting module in the first imaging channel is used for receiving the converged light; The second imaging channel further comprises a second sleeve lens and a second filter arranged on the transmission light path of the second dichroic mirror, the second sleeve lens is used for converging light, the second filter is a single-band pass filter, and only allows light of the fluorescence wave band of T base to pass through, and the shooting module in the second imaging channel is used for receiving the converged light.
7. The genetic sequencing optical system of claim 2, wherein, The dichroic mirror set further comprises a third dichroic mirror; The third dichroic mirror is arranged on an outgoing light path of the transmission light of the second dichroic mirror, and the third dichroic mirror separates the imaging light transmitted through the second dichroic mirror to form multi-channel imaging light.
8. The genetic sequencing optical system of claim 7, wherein, The imaging channels include a first imaging channel, a second imaging channel and a third imaging channel. The second dichroic mirror can reflect light in a wavelength range covering the fluorescence wavelength band of A bases and can transmit light in a wavelength range covering the fluorescence wavelength bands of T bases and G bases. The third dichroic mirror can reflect light in a wavelength range covering the fluorescence wavelength band of G bases and can transmit light in a wavelength range covering the fluorescence wavelength band of T bases. The first imaging channel is located on the reflected light path of the second dichroic mirror, the second imaging channel is located on the transmitted light path of the third dichroic mirror, and the third imaging channel is located on the reflected light path of the third dichroic mirror.
9. The genetic sequencing optical system of claim 8, wherein, The first imaging channel further includes a first sleeve lens and a first filter located on the reflected light path of the second dichroic mirror, the first sleeve lens is used for converging light, the first filter is a single band-pass filter that only allows light in the fluorescence wavelength band of A bases to pass, and the shooting module in the first imaging channel is used for receiving the converged light. The second imaging channel further includes a second sleeve lens and a second filter located on the transmitted light path of the second dichroic mirror, the second sleeve lens is used for converging light, the second filter is a single band-pass filter that only allows light in the fluorescence wavelength band of T bases to pass, and the shooting module in the second imaging channel is used for receiving the converged light. The third imaging channel further includes a third sleeve lens and a third filter located on the reflected light path of the second dichroic mirror, the third sleeve lens is used for converging light, the third filter is a single band-pass filter that only allows light in the fluorescence wavelength band of G bases to pass, and the shooting module in the third imaging channel is used for receiving the converged light.
10. A genetic sequencer, characterized by, The gene sequencing optical system includes a displacement table and the gene sequencing optical system according to any one of claims 1 to 9. The displacement table is used for carrying a sequencing chip and carrying the sequencing chip to move so that the gene sequencing optical system images different base samples on the sequencing chip.