Device for analyzing chemotactic factors

Abstract

The utility model relates to a device for analyzing chemotactic factors, which comprises a substrate, and a culture substrate and a main invasion channel communicated with the culture substrate are arranged on the substrate; a plurality of secondary invasion channels are connected to the main invasion channel in a penetrating manner, and matrigel is injected into the main invasion channel and the secondary invasion channels; a storage groove is formed in the tail end of each secondary invasion channel, and different chemotactic factors are placed in every two adjacent storage grooves. Through the design of the secondary invasion channel, matrigel is added into the primary invasion channel and the secondary invasion channel, and chemotactic factors of different types or different concentrations are placed in each storage groove; cells invade the matrigel under the action of the chemotactic factors, and distribution of the cells in the matrigel in a secondary invasion channel can be more intuitively observed by utilizing image analysis and fluorescent staining analysis, so that the influence of the chemotactic factors of different types or different concentrations on the effect that the cells invade the matrigel can be analyzed.

Description

TECHNICAL FIELD

[0001] The utility model relates to biomedical experiment instrument technical field especially a device for analyzing chemotactic factor. BACKGROUND

[0002] Chemotactic factor: it is a kind of small cell factor or signal protein by cell secretion, because they have the ability of inducing nearby reaction cell directional chemotaxis, therefore plays a decisive role in the process of analyzing cell migration and invasion, therefore analyzing the influence of different kinds and different concentrations of chemotactic factor on same cell is the indispensable work in biomedical experiment.

[0003] A tumor cell in vitro invasion ability analysis chip is disclosed in Chinese patent with publication number CN220643053U, which comprises a bottom sheet and a cover sheet bonded with the bottom sheet, a cell loading pool, a plurality of chemotactic factor loading pools and an invasion microchannel are formed between the bottom sheet and the cover sheet, the plurality of chemotactic factor loading pools are communicated with the cell loading pool through independent invasion microchannels respectively; the cover sheet is provided with a matrix adding port at the position of the invasion microchannel. The chip can be used to analyze the invasion ability of tumor cells on different tissues such as liver, lung, bone, etc., and is simple to use. By using the above technical solution, different types of chemotactic factors are placed in different chemotactic factor loading pools, and the same matrix glue is placed in each invasion microchannel. Then, by using image analysis and fluorescence staining analysis, the distribution of cells in the matrix glue in the invasion microchannel can be more directly observed. Thus, the influence of different types and different concentrations of chemotactic factors on cell invasion can be analyzed inversely. However, in the above technical solution, only the influence of chemotactic factors on cell invasion of the same type of matrix glue can be analyzed. The influence of chemotactic factors on cell invasion of different types of matrix glue cannot be analyzed. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a device for analyzing chemotactic factor to solve the problems in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model adopts the technical scheme of: a device for analyzing chemotactic factor, comprising a substrate, a culture medium and a main invasion channel connected through the culture medium are arranged on the substrate; a plurality of secondary invasion channels are connected through the main invasion channel, and matrix glue is injected into the main invasion channel and the secondary invasion channel; a storage groove is arranged at the end of each secondary invasion channel, and different chemotactic factors are placed in adjacent two storage grooves.

[0006] Compared with the prior art, by the design of the secondary invasion channel and the addition of the matrix glue in the primary invasion channel and the secondary invasion channel, and by placing different kinds or different concentrations of chemotactic factors in each storage groove, cells invade the matrix glue under the action of the chemotactic factors, image analysis and fluorescent staining analysis can be used to more directly observe the distribution of the cells in the matrix glue in the secondary invasion channel, and the influence of different kinds or different concentrations of chemotactic factors on the cell invasion of the matrix glue can be analyzed.

[0007] The technical scheme of the utility model discloses, the primary invasion channel and the secondary invasion channel inject same or different matrix glue.

[0008] Compared with the prior art, when injecting different kinds of matrix glue in the primary invasion channel and the secondary invasion channel, and placing different kinds or different concentrations of chemotactic factors in each storage groove, cells invade the matrix glue under the action of the chemotactic factors, image analysis and fluorescent staining analysis can be used to more directly observe the distribution of the cells in the matrix glue in the secondary invasion channel, and the influence of different kinds or different concentrations of chemotactic factors on the cell invasion of the two different kinds of matrix glue can be analyzed.

[0009] The technical scheme of the utility model discloses, still be provided with the guide base body with the primary invasion channel through connection on the base plate, and the guide base body and the culture base body are located at both ends of the primary invasion channel.

[0010] The technical scheme of the utility model discloses, a plurality of secondary invasion channels are arranged on one side of the primary invasion channel.

[0011] The technical scheme of the utility model discloses, the secondary invasion channel and the primary invasion channel are T-shaped through connection.

[0012] The technical scheme of the utility model discloses, the both sides of the primary invasion channel are arranged with a plurality of secondary invasion channels.

[0013] The technical scheme of the utility model discloses, the secondary invasion channel is branchy or symmetrically arranged on both sides of the primary invasion channel.

[0014] The technical scheme of the utility model discloses, the secondary invasion channel width is 400um-600mm, and the depth is 100-110um.

[0015] The technical scheme of the utility model discloses, the material of the base body is any one of PC, COC, PMMA, PDMS

[0016] In addition to the technical problems solved by the application described above, the technical features constituting the technical scheme, and the advantages brought by these technical features, other technical problems solved by the application, other technical features included in the technical scheme, and the advantages brought by these technical features will be further described in detail with reference to the drawings. Attached Figure Description

[0017] Figure 1 This is a cross-sectional view of one embodiment of the present utility model.

[0018] Figure 2 This is a cross-sectional view of adding the same matrix gel to the main and secondary invasion channels.

[0019] Figure 3 It is a cross-sectional view of adding different matrix adhesives in the main invasion channel and the secondary invasion channel.

[0020] Figure 4 This is a cross-sectional view of Embodiment 2 of this utility model.

[0021] Figure 5 This is a cross-sectional view of Embodiment 3 of this utility model.

[0022] Explanation of reference numerals: 01. Substrate, 02. Culture medium, 03. Main invasion channel, 04. Secondary invasion channel, 05. Storage tank, 06. Guide substrate. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0024] Example 1, please refer to Figures 1-3 As shown, the device for analyzing chemokines provided by this invention is mainly used to analyze the effects of different chemokines on cell invasion. It includes a substrate 01, on which a culture medium 02 and a main invasion channel 03 connected and communicating with the culture medium 02 are disposed. The culture medium 02 is used for seeding cells. To intuitively understand the effects of different concentrations or types of chemokines on cell invasion, multiple secondary invasion channels 04 are provided on one side of the main invasion channel 03. The secondary invasion channels 04 are T-shaped and connected to the main invasion channel 03. A storage groove 05 is provided at the end of each secondary invasion channel 04.

[0025] The first method involves injecting the same type of matrix adhesive (such as...) into both the main invasion microchannel and the secondary invasion channel 04. Figure 2 (As shown), cells were then seeded in culture medium 02, and different types of chemokines were placed in each storage tank 05. Cells invaded the matrix gel under the influence of the chemokines. Image analysis and fluorescence staining analysis allowed for a more direct observation of cell distribution within the matrix gel. This revealed the effect of different types or concentrations of chemokines on cell invasion within the same matrix gel.

[0026] The same way in each storage tank 05 placed different concentrations of chemotactic factor, using image analysis and fluorescence staining analysis, can be more intuitive to observe the distribution of cells in the matrix gel. Can analyze the influence of different concentrations of chemotactic factor on cell invasion in the same matrix gel.

[0027] The second use, in the secondary invasion channel 04 injection and the main invasion micro-channel in different kinds of matrix gel (such as Figure 3 shown). Then the cells are inoculated in the culture medium 02, in each storage tank 05 placed different kinds of chemotactic factor. Cells in the chemotactic effect in different kinds of matrix gel invasion. With image analysis and fluorescence staining analysis, can be more intuitive to observe the distribution of cells in the matrix gel. So as to analyze the influence of different kinds of chemotactic factor on cell invasion in two kinds of matrix gel.

[0028] The same way in each storage tank 05 placed different concentrations of chemotactic factor, using the same method, can be obtained in different concentrations of chemotactic factor on cell invasion in two kinds of matrix gel.

[0029] At the same time in the secondary invasion channel 04 and the main invasion channel 03 injection of different kinds of matrix gel, also can simulate the invasion of cells in different tissues in the human body.

[0030] The above secondary invasion channel 04 width is 400um-600mm, depth is 100-110um, in this embodiment, preferably the secondary invasion channel width is 500um, depth is 105um.

[0031] The material of the matrix 02 is any one of PC, COC, PMMA, PDMS, the material of the matrix 02 is preferably used PDMS.

[0032] Example two, please refer to Figure 4 shown, the difference between this embodiment and the above embodiment is that the main invasion channel 03 both sides are arranged with a plurality of secondary invasion channel 04. The end of each secondary invasion channel 04 is provided with a storage tank 05, the secondary invasion channel 04 is dendritic or symmetrically arranged on both sides of the main invasion channel 03. The secondary invasion channel 04 is arranged on both sides of the main invasion channel 03 can be used to simulate the relationship between the human body blood vessels and human tissues. At the same time in the actual application process, the main invasion channel and the secondary invasion channel located on both sides of the main invasion channel are added with different kinds of matrix gel, and different kinds or different concentrations of chemotactic factor are placed in the storage tank 05. Using image analysis and fluorescence staining analysis, can be more intuitive to observe the distribution of cells in the secondary invasion channel. So as to analyze the influence of different kinds and different concentrations of chemotactic factor on cell invasion.

[0033] That is, cells A are inoculated in the culture base 02, the main invasion channel 03 is filled with matrix glue A, the secondary invasion channel 04 located on one side of the main invasion channel 03 is filled with matrix glue B, and the secondary invasion channel 04 located on the other side of the main invasion channel 03 is filled with matrix glue C. Meanwhile, different kinds or different concentrations of chemotactic factors are placed in the storage tank 05. Image analysis and fluorescent staining analysis are used to obtain the distribution of cells that invade and migrate from the matrix glue A to the matrix glue B, and the distribution of cells that invade and migrate from the matrix glue A to the matrix glue C. Then, the influence of different kinds and different concentrations of chemotactic factors on cell invasion is reversely analyzed.

[0034] For example, the matrix glue placed in the secondary invasion channel located on one side of the main invasion channel is the same as the matrix glue in the main invasion channel, the matrix glue placed in the secondary invasion channel located on the other side of the main invasion channel is different from the matrix glue in the main invasion channel, and different kinds or different concentrations of chemotactic factors are placed in the storage tank 05. Image analysis and fluorescent staining analysis can be used to more directly observe the distribution of cells in the secondary invasion channel. Thus, the influence of different kinds and different concentrations of chemotactic factors on cell invasion is reversely analyzed.

[0035] That is, cells A are inoculated in the culture base 02, the main invasion channel 03 is filled with matrix glue A, the secondary invasion channel 04 located on one side of the main invasion channel 03 is filled with matrix glue A, and the secondary invasion channel 04 located on the other side of the main invasion channel 03 is filled with matrix glue B. Meanwhile, different kinds or different concentrations of chemotactic factors are placed in the storage tank 05. Image analysis and fluorescent staining analysis are used to obtain the distribution of cells that invade and migrate in the matrix glue A, and the distribution of cells that invade and migrate from the matrix glue A to the matrix glue B. Then, the influence of different kinds and different concentrations of chemotactic factors on cell invasion is reversely analyzed.

[0036] For example, the matrix glue placed in the secondary invasion channel located on one side of the main invasion channel is the same as the matrix glue in the main invasion channel, the matrix glue placed in the secondary invasion channel located on the other side of the main invasion channel is different from the matrix glue in the main invasion channel, and different kinds or different concentrations of chemotactic factors are placed in the storage tank 05. Image analysis and fluorescent staining analysis can be used to more directly observe the distribution of cells in the secondary invasion channel. Thus, the influence of different kinds and different concentrations of chemotactic factors on cell invasion is reversely analyzed. Figure 5 The difference between the embodiment and the above-mentioned embodiments one and two is that the guide base is further arranged on the substrate 01 and is connected with the main invasion channel, and the guide base and the culture base 02 are located at two ends of the main invasion channel 03. Different kinds or different concentrations of matrix glues are added in the main invasion channel and the secondary invasion channel. Different kinds or different concentrations of chemotactic factors are placed in the guide base and the storage tank. The chemotactic factor in the guide base provides initial guidance for cell invasion.

[0037] If the embodiments of the utility model have directionality indication (such as up, down, left, right, front, back...), then the directionality indication is only used to explain the relative position relationship, movement condition and the like between components in a certain specific posture (as shown in the drawings), if the specific posture changes, then the directionality indication also changes accordingly.

[0038] The above embodiments only describe the preferred embodiments of the utility model, and do not limit the scope of the utility model, and various deformations and improvements of the technical scheme of the utility model made by the ordinary engineering technicians in the art without departing from the design spirit of the utility model shall fall within the protection scope determined by the claims of the utility model.

Claims

1. An apparatus for analyzing chemokines, comprising a substrate, wherein a culture medium and a main invasion channel communicating with the culture medium are disposed on the substrate; characterized in that, Multiple secondary invasion channels are connected to the main invasion channel. Both the main and secondary invasion channels are filled with matrix gel. Each secondary invasion channel has a storage tank at its end, and different chemotactic factors are placed in adjacent storage tanks.

2. The apparatus for analyzing chemokines according to claim 1, characterized in that: The main and secondary invasion channels are injected with the same or different matrix adhesives.

3. The apparatus for analyzing chemokines according to claim 1, characterized in that: Multiple secondary attack channels are arranged on one side of the main attack channel.

4. The apparatus for analyzing chemokines according to claim 1, characterized in that: Multiple secondary attack channels are arranged on both sides of the main attack channel.

5. The apparatus for analyzing chemokines according to claim 4, characterized in that: Secondary attack channels are arranged in a tree-like or symmetrical manner on both sides of the main attack channel.

6. The apparatus for analyzing chemokines according to any one of claims 1-5, characterized in that: The substrate is also provided with a guide substrate that is connected to the main invasion channel. The guide substrate and the culture medium are located at both ends of the main invasion channel.

7. The apparatus for analyzing chemokines according to any one of claims 1-5, characterized in that: The width of the secondary invasion channel is 400um-600mm, and the depth is 100-110um.

8. The apparatus for analyzing chemokines according to claim 1, characterized in that: The matrix material can be any one of PC, COC, PMMA, or PDMS.

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