Ultrafiltration, purification and concentration device for recombinant antigen

Abstract

The utility model discloses a recombinant antigen ultrafiltration purification and concentration device, which belongs to the technical field of biological medicine and comprises a storage tank and a storage tank, the top of the storage tank is provided with a first guide pipe and a second connecting pipe which are provided with electromagnetic valves, and the top of the storage tank is provided with a second guide pipe and a first connecting pipe which are provided with electromagnetic valves; the filter is arranged between a storage tank and a storage tank, the top surface of the filter is respectively communicated with a guide pipe I, a guide pipe II, a connecting pipe I and a connecting pipe II, the peripheral side of the guide pipe II is communicated with the storage tank, and the peripheral side of the connecting pipe II is communicated with the storage tank; the ends, far away from the filter, of the guide pipe I and the connecting pipe I are respectively communicated with a first pump body and a second pump body, and the input ends of the first pump body and the second pump body are respectively communicated with the storage tank and the storage tank.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the biological medicine technical field, especially a kind of recombinant antigen ultrafiltration purification concentration device. BACKGROUND

[0002] Recombinant antigen processing needs to use ultrafiltration purification concentration device, so that the filter of ultrafiltration purification concentration device carries out ultrafiltration purification concentration to recombinant antigen, the existing ultrafiltration purification concentration device when using, adopt the way of circulation to carry out multiple ultrafiltration purification concentration to recombinant antigen, and after the backflow of recombinant antigen ultrafiltration purification concentration, it is mixed with the recombinant antigen that has not carried out ultrafiltration purification concentration, reduce the ultrafiltration purification concentration quality of ultrafiltration purification concentration device to recombinant antigen.

[0003] In the related art, through retrieval, it is found that a kind of ultrafiltration concentration device in Chinese patent document (grant announcement number CN217887551U), including the harvest liquid bottle, clarifier, transfer bottle, filter and sample bottle that are sequentially communicated by pipeline;First conveying pump is arranged between harvest liquid bottle and clarifier, second conveying pump is arranged between transfer bottle and filter;First Y type pipe fitting is arranged on the pipeline between sample bottle and filter, the third end of first Y type pipe fitting is communicated with transfer bottle by pipeline;First valve is arranged on the pipeline that first Y type pipe fitting is communicated with transfer bottle, second valve is arranged on the pipeline that first Y type pipe fitting is communicated with sample bottle.By adopting the above technical scheme, clarification, concentration are sequentially carried out in device, realize synchronization, save time, while realize when using buffer to wash elution to pipeline, solution in sample bottle does not need to be transferred, avoid the pollution caused by pipetting, but the device does not consider that, during the process of circulating ultrafiltration purification concentration recombinant antigen, the ultrafiltration purification concentrated recombinant antigen mixes with the recombinant antigen that has not carried out ultrafiltration purification concentration after backflow, reduce the ultrafiltration purification concentration quality of device to recombinant antigen. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of recombinant antigen ultrafiltration purification concentration device to solve the problems raised in the background art.

[0005] To achieve the above object, the utility model provides the following technical scheme: a kind of recombinant antigen ultrafiltration purification concentration device, comprising:

[0006] storage tank and storage tank for recombinant antigen ultrafiltration purification concentration, storage tank top is provided with the conduit one with electromagnetic valve and connecting pipe two, and storage tank top is provided with the conduit two with electromagnetic valve and connecting pipe one;

[0007] The filter for recombination antigen ultrafiltration purification concentration is arranged between a storage tank and a storage tank, the top surface of the filter is communicated with a guide pipe one, a guide pipe two, a connecting pipe one and a connecting pipe two, the outer circumferential side of the guide pipe two is communicated with the storage tank, the outer circumferential side of the connecting pipe two is communicated with the storage tank, the guide pipe one and the connecting pipe one are respectively communicated with a first pump body and a second pump body at the end away from the filter, and the input ends of the first pump body and the second pump body are respectively communicated with the storage tank and the storage tank.

[0008] Preferably, the filter is provided with a chromatography column and an ultrafiltration membrane filter column, and the chromatography column and the ultrafiltration membrane filter column are sequentially arranged along the flow direction of the recombination antigen.

[0009] Preferably, the ultrafiltration membrane filter column comprises an external pressure ultrafiltration membrane filter column and an internal pressure ultrafiltration membrane filter column, the chromatography column is connected with the internal pressure ultrafiltration membrane filter column, and the external pressure ultrafiltration membrane filter column is connected with the storage tank.

[0010] The filter is provided with a pressurizing device, the recombination antigen in the cavity is accelerated to pass through the external pressure ultrafiltration membrane filter column by pressurizing the inner cavity of the filter.

[0011] Preferably, the pressure-reducing assembly comprises a fixed pipe and a connecting ring with a threaded inner annular surface, and the outer circumferential side of the left fixed pipe is communicated with the storage tank.

[0012] Preferably, the outer circumferential side of the right fixed pipe is communicated with the storage tank, and the inner pipe surface of the fixed pipe is connected with an air film for balancing the internal and external air pressures.

[0013] Preferably, the outer surface of the fixed pipe is connected with a threaded ring, and the threaded ring is threadedly connected with the connecting ring.

[0014] Preferably, the top surface of the connecting ring is connected with four connecting plates arranged at equal intervals, and the top of the air film is provided with a conical shielding plate.

[0015] Preferably, the top surfaces of the four connecting plates are connected and fixed with the shielding plate.

[0016] Compared with the prior art, the technical effects and advantages of the utility model are as follows:

[0017] The recombination antigen ultrafiltration purification and concentration device, the recombination antigen needs to be ultrafiltration purified and concentrated during processing, and the quality of the ultrafiltration purification and concentration of the recombination antigen is improved by repeatedly performing the ultrafiltration purification and concentration in cycles, and the recombination antigen that flows back after the completion of the ultrafiltration purification and concentration is mixed with the recombination antigen that has not been subjected to the ultrafiltration purification and concentration, so that the quality of the ultrafiltration purification and concentration of the recombination antigen is low, the first pump body is arranged to extract the recombination antigen in the storage tank, the recombination antigen is introduced into the filter through the first conduit, the recombination antigen that is ultrafiltration purified and concentrated by the filter is introduced into the storage tank through the second conduit, after the recombination antigen in the storage tank is ultrafiltration purified and concentrated, the second pump body is powered, and the ultrafiltration purification and concentration are performed again under the communication of the first connecting pipe and the second connecting pipe, so that the processing quality of the recombination antigen is improved;

[0018] The recombination antigen ultrafiltration purification and concentration device, the two pressure reduction assemblies are arranged to balance the internal and external air pressure of the storage tank and the storage tank, prevent the negative pressure from occurring in the storage tank and the storage tank, the air film is arranged to filter the sundries in the external environment, prevent the sundries in the external environment from flowing into the recombination antigen, the surface structure of the air film is precise and is easily damaged, the inner thread of the connecting ring moves along the thread path of the thread ring, the shielding plate is located on the upper portion of the air film during the transmission process, the air film is shielded and protected, and the air film is prevented from being damaged, the connecting plate provides a supporting effect, and a gap exists between the shielding plate and the air film, so that the flow of air is provided with conditions;

[0019] The recombination antigen ultrafiltration purification and concentration device, the electromagnetic valves in the first conduit and the second conduit are synchronously opened and closed, the electromagnetic valves in the first connecting pipe and the second connecting pipe are synchronously opened and closed, when the recombination antigen in the storage tank flows towards the storage tank, the electromagnetic valves in the first conduit and the second conduit are opened, and the electromagnetic valves in the first connecting pipe and the second connecting pipe are closed, when the recombination antigen in the storage tank flows towards the storage tank, the electromagnetic valves in the first connecting pipe and the second connecting pipe are opened, and the electromagnetic valves in the first conduit and the second conduit are closed. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the following will briefly introduce the drawings needed to be used in the specific embodiments or the prior art description. Obviously, the drawings described below are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0021] Fig. 1 It is a structural schematic view of the present application;

[0022] Fig. 2 It is a structural schematic view of the pressure reduction assembly of the present application;

[0023] Fig. 3 It is the split structure schematic view of the fixed pipe and the shielding plate of the utility model;

[0024] Fig. 4 It is another view structure schematic view of the shielding plate of the utility model.

[0025] Explanation of reference signs:

[0026] In the figure: 1, storage tank; 2, filter; 3, storage tank; 4, conduit one; 5, conduit two; 6, connecting pipe one; 7, connecting pipe two; 8, first pump body; 9, second pump body; 10, feed inlet; 11, discharge outlet; 12, pressure reducing assembly; 13, fixed pipe; 14, air film; 15, threaded ring; 16, connecting ring; 17, connecting plate; 18, shielding plate. Specific implementation

[0027] In the following description, a large number of specific details are given in order to provide a more thorough understanding of the utility model. However, it is obvious to those skilled in the art that the utility model can be implemented without one or more of these details. In other examples, in order to avoid obscuring the utility model, some technical features known in the art are not described.

[0028] Unless the direction indicated by the single definition, the directions such as up, down, left, right, front, back, inside and outside involved in this paper are based on the directions such as up, down, left, right, front, back, inside and outside in the figure shown by the utility model, which is explained here.

[0029] The connection mode can adopt existing modes such as bonding, welding, bolt connection and the like, and the actual needs are accurate.

[0030] In order to solve the problem that the ultrafiltration purification and concentration quality of recombinant antigen is low after the recombinant antigen is ultrafiltrated and purified and concentrated for multiple times in the recombinant antigen ultrafiltration purification and concentration device, please refer to Figs. 1 to 4 The structure of the device body is as follows:

[0031] The storage tank 1 and the storage tank 3 for ultrafiltration purification and concentration of recombinant antigen are provided with conduit one 4 and connecting pipe two 7 with electromagnetic valves at the top of the storage tank 1, and the storage tank 3 is provided with conduit two 5 and connecting pipe one 6 with electromagnetic valves at the top;

[0032] The filter 2 is arranged between the storage tank 1 and the storage tank 3, and the filter 2 is provided with a first conduit 4, a second conduit 5, a first connecting pipe 6 and a second connecting pipe 7. The second conduit 5 is communicated with the storage tank 3, and the second connecting pipe 7 is communicated with the storage tank 1. The first pump body 8 and the second pump body 9 are respectively communicated with the first conduit 4 and the second conduit 5.

[0033] In the embodiment, the filter 2 is used for ultrafiltration purification and concentration of the recombinant antigen. The existing ultrafiltration purification and concentration device usually adopts continuous ultrafiltration purification and concentration to improve the ultrafiltration purification and concentration quality of the recombinant antigen. When the recombinant antigen that has completed the ultrafiltration purification and concentration flows back, it is mixed with the recombinant antigen that has not been subjected to the ultrafiltration purification and concentration, thereby reducing the ultrafiltration purification and concentration quality of the recombinant antigen. The first pump body 8 is used to extract the recombinant antigen in the storage tank 1. At this time, the electromagnetic valves in the first connecting pipe 6 and the second connecting pipe 7 are closed, and the electromagnetic valves in the first conduit 4 and the second conduit 5 are opened, so that the recombinant antigen flows through the filter 2 for ultrafiltration purification and concentration and is introduced into the storage tank 3. When the ultrafiltration purification and concentration of the recombinant antigen in the storage tank 1 is completed, the second pump body 9 extracts the recombinant antigen in the storage tank 3. At this time, the electromagnetic valves in the first connecting pipe 6 and the second connecting pipe 7 are opened, and the electromagnetic valves in the first conduit 4 and the second conduit 5 are closed, so that the recombinant antigen flows through the filter 2 for re-ultrafiltration purification and concentration, and then flows into the storage tank 1. The ultrafiltration purification and concentration device is more thorough for the ultrafiltration purification and concentration of the recombinant antigen, thereby improving the ultrafiltration purification and concentration quality of the recombinant antigen. The filter 2 is divided into two space parts, the first conduit 4 and the second conduit 5 are communicated with the same space part of the filter 2, and the first connecting pipe 6 and the second connecting pipe 7 are communicated with the same space part of the filter 2.

[0034] The filter 2 is provided with a chromatographic column and an ultrafiltration membrane filter column. The chromatographic column and the ultrafiltration membrane filter column are arranged in sequence along the flow direction of the recombinant antigen. In order to improve the ultrafiltration concentration effect, the ultrafiltration membrane filter column includes an external pressure ultrafiltration membrane filter column and an internal pressure ultrafiltration membrane filter column. The chromatographic column is connected with the internal pressure ultrafiltration membrane filter column, and the external pressure ultrafiltration membrane filter column is connected with the storage tank. In this way, the antigen is purified by the chromatographic column, and then subjected to ultrafiltration concentration by the internal pressure ultrafiltration membrane filter column and released into the filter cavity. The once-ultrafiltration-concentrated antigen passes through the external pressure ultrafiltration membrane filter column again and enters the storage tank 3. When the recombinant antigen in the storage tank 3 flows through the filter 2 again, it is subjected to ultrafiltration concentration by the external pressure ultrafiltration membrane filter column again, thereby obtaining highly concentrated recombinant antigen.

[0035] In some embodiments, a pressurizing device is arranged on the filter 2, and by pressurizing the inner cavity of the filter 2, the rate of the antigen passing through the outer pressure ultrafiltration membrane filter column in the cavity is accelerated, and the working efficiency of the ultrafiltration purification and concentration device is improved.

[0036] Specifically, the storage tank 1 is respectively provided with a feed inlet 10 and a discharge outlet 11 with electromagnetic valves on the side, and the storage tank 1 and the storage tank 3 are respectively provided with pressure relief components 12 on the top surface to balance the internal and external air pressure.

[0037] In this embodiment, the feed inlet 10 and the discharge outlet 11 are respectively used to introduce the recombinant antigen to be ultrafiltration purified and concentrated and to discharge the recombinant antigen after ultrafiltration purification and concentration, and the internal air pressure of the storage tank 1 and the storage tank 3 changes during the conveying of the ultrafiltration purified and concentrated recombinant antigen. The two pressure relief components 12 are arranged to balance the internal and external air pressure of the storage tank 1 and the storage tank 3, thereby improving the structural stability of the parts of the device.

[0038] Specifically, the pressure relief component 12 comprises a fixed tube 13 and a connecting ring 16 with threads on the inner ring surface. The outer circumferential side of the left fixed tube 13 is in communication with the storage tank 1, and the outer circumferential side of the right fixed tube 13 is in communication with the storage tank 3. The inner tube surface of the fixed tube 13 is connected with an air film 14 for balancing the internal and external air pressure. The outer surface of the fixed tube 13 is connected with a threaded ring 15, and the threaded ring 15 is threadedly connected with the connecting ring 16. The top surface of the connecting ring 16 is connected with four equidistantly arranged connecting plates 17. The top of the air film 14 is provided with a conical-shaped shielding plate 18, and the top surface of the four connecting plates 17 is connected and fixed with the shielding plate 18.

[0039] In this embodiment, under the connection of the fixed tube 13 and the air film 14, the outside air flows into the storage tank 1 or the storage tank 3, and the gas inside the storage tank 1 and the storage tank 3 flows out. The air film 14 is arranged to shield external impurities and prevent the external impurities from flowing in and contaminating the recombinant antigen. Since the air film 14 is structurally precise and is easily damaged, the shielding plate 18 is arranged to shield and protect the air film 14, thereby improving the structural stability of the parts of the device. The outer ring surface of the threaded ring 15 has threads, the inner ring surface of the connecting ring 16 has threads, and the threads of the threaded ring 15 and the threads of the connecting ring 16 are engaged, so that the connecting ring 16 moves downward along the thread path of the threaded ring 15. When it moves to the specified position, it is fixed, so that the shielding plate 18 is located above the air film 14 for shielding. The four connecting plates 17 provide support, so that there is a gap between the shielding plate 18 and the air film 14, which facilitates the flow of air and improves the structural stability of the parts of the device.

[0040] The filter 2, the first pump body 8, the second pump body 9 and the electromagnetic valve are prior arts, and the working principles, sizes and models are irrelevant to the functions of the present application, so no more description is made, the control mode of the present application is controlled by the controller, the control circuit of the controller can be realized by simple programming of the person skilled in the art, the power supply also belongs to the common knowledge in the art, and the present application is mainly used for protecting the mechanical device, so the control mode and the circuit connection of the present application will not be explained in detail.

[0041] The working principle of the recombinant antigen ultrafiltration purification and concentration device of the present application will be introduced below. When the user uses the recombinant antigen ultrafiltration purification and concentration device, the electromagnetic valves inside the conduit one 4 and the conduit two 5 are separately opened, the first pump body 8 is started to draw the recombinant antigen in the storage tank 1, the recombinant antigen flows into the filter 2 for ultrafiltration purification and concentration, and then flows into the storage tank 3;

[0042] When the ultrafiltration purification and concentration of the recombinant antigen in the storage tank 1 is completed, the electromagnetic valves inside the conduit one 4 and the conduit two 5 are closed, the electromagnetic valves inside the connecting pipe one 6 and the connecting pipe two 7 are separately opened, the second pump body 9 draws the recombinant antigen in the storage tank 3, the recombinant antigen flows into the filter 2 for re-ultrafiltration purification and concentration, and then flows into the storage tank 1;

[0043] The connecting ring 16 is moved downward along the thread path of the thread ring 15, under the support of the four connecting plates 17, the shielding plate 18 is located at the upper part of the air film 14, the air film 14 is shielded and protected, and the air film 14 is prevented from being damaged in the use process.

[0044] It should be noted that, in this text, relational terms such as one and two are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variant thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment. Without more limitations. The statement "including a limited element" does not exclude the existence of another identical element in the process, method, article or equipment including the element.

[0045] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A recombinant antigen ultrafiltration purification and concentration device, characterized in that, include: Storage tank (1) and storage tank (3) for ultrafiltration purification and concentration of recombinant antigens, wherein the top of the storage tank (1) is provided with a conduit (4) with a solenoid valve and a connecting pipe (7), and the top of the storage tank (3) is provided with a conduit (5) with a solenoid valve and a connecting pipe (6). A filter (2) for ultrafiltration purification and concentration of recombinant antigens is provided between a storage tank (1) and a storage tank (3). The top surface of the filter (2) is connected to a first conduit (4), a second conduit (5), a first connecting tube (6), and a second connecting tube (7). The outer periphery of the second conduit (5) is connected to the storage tank (3), and the outer periphery of the second connecting tube (7) is connected to the storage tank (1). The ends of the first conduit (4) and the first connecting tube (6) away from the filter (2) are connected to a first pump body (8) and a second pump body (9), respectively. The input ends of the first pump body (8) and the second pump body (9) are connected to the storage tank (1) and the storage tank (3), respectively.

2. The recombinant antigen ultrafiltration purification and concentration device according to claim 1, characterized in that: The filter (2) is provided with a chromatography column and an ultrafiltration membrane filter column, which are arranged sequentially along the flow direction of the recombinant antigen.

3. The recombinant antigen ultrafiltration purification and concentration device according to claim 2, characterized in that: The ultrafiltration membrane filter column includes an external pressure ultrafiltration membrane filter column and an internal pressure ultrafiltration membrane filter column. The chromatography column is connected to the internal pressure ultrafiltration membrane filter column, and the external pressure ultrafiltration membrane filter column is connected to the storage tank (3).

4. The recombinant antigen ultrafiltration purification and concentration device according to claim 3, characterized in that: The filter (2) is equipped with a pressurizing device, which pressurizes the inner cavity of the filter (2) to accelerate the rate at which the recombinant antigen in the cavity passes through the external pressure ultrafiltration membrane filter column.

5. The recombinant antigen ultrafiltration purification and concentration device according to claim 1, characterized in that: The storage tank (1) is equipped with a feed inlet (10) and a discharge outlet (11) with solenoid valves on its side. The top surfaces of the storage tank (1) and the storage tank (3) are equipped with pressure reducing components (12) to balance the internal and external air pressure.

6. The recombinant antigen ultrafiltration purification and concentration apparatus according to claim 5, characterized in that: The pressure-reducing assembly (12) includes a fixed tube (13) and a connecting ring (16) with threads on its inner ring surface. The outer periphery of the fixed tube (13) on the left is connected to the storage tank (1).

7. The recombinant antigen ultrafiltration purification and concentration apparatus according to claim 6, characterized in that: The outer periphery of the fixed tube (13) on the right is connected to the storage tank (3), and the inner surface of the fixed tube (13) is connected to an air film (14) to balance the internal and external air pressure.

8. The recombinant antigen ultrafiltration purification and concentration apparatus according to claim 7, characterized in that: The fixed tube (13) has a threaded ring (15) connected to its outer surface, and the threaded ring (15) is threadedly connected to the connecting ring (16).

9. The recombinant antigen ultrafiltration purification and concentration apparatus according to claim 8, characterized in that: The top surface of the connecting ring (16) is connected to four equidistant connecting plates (17), and the top of the air film (14) is provided with a cone-shaped baffle plate (18).

10. The recombinant antigen ultrafiltration purification and concentration apparatus according to claim 9, characterized in that: The top surfaces of the four connecting plates (17) are connected and fixed to the shielding plate (18).

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