One-Pot reaction device

Abstract

The utility model belongs to the field of One-Pot reaction, particularly relates to an One-Pot reaction device, and provides the following scheme aiming at the problems that the existing device cannot realize integrated detection of multiple targets, and in addition, the uncovering treatment of amplification products increases potential risk of cross contamination of aerosol and the operation is tedious, the One-Pot reaction device comprises a reaction tube, a cross-shaped light blocking plate is fixedly arranged in the reaction tube, the reaction tube is divided into four areas through the cross-shaped light blocking plate, a sealing plate is fixedly arranged at the top of the cross-shaped light blocking plate, four sample adding holes are formed in the top of the sealing plate, and reagent storage bags are arranged in the four areas on the inner wall of the reaction tube. Through the arrangement of the cross-shaped light blocking plate and the reagent storage bag, effective separation of multi-target reaction and avoidance of light pollution are realized, meanwhile, reagents are automatically released by utilizing centrifugal force, the operation is simple and convenient, and the pollution risk of aerosol carried by frequently opening and closing the cover is reduced.

Description

Technical Field

[0001] This utility model relates to the field of One-Pot reaction technology, and in particular to a One-Pot reaction apparatus. Background Technology

[0002] One-pot reactions are based on the similarity of optimal reaction temperatures, seamlessly integrating different reaction systems into a single device to save reaction time and improve the speed of detection. However, due to the differences and incompatibilities of different reaction system enzymes and buffer reagents, mutual interference problems greatly limit the application of one-pot reactions in practical scenarios.

[0003] In recent years, although studies have optimized the buffer substances and / or DNA / RNA templates for different reaction systems of One-Pot, interference problems such as the incompatibility of components in multiple systems remain the biggest obstacles to the further application of One-Pot (storage, transportation, competitive inhibition, etc.). In particular, it cannot achieve integrated detection of multiple targets. In addition, the opening and handling of amplification products increases the potential risk of aerosol cross-contamination and the complexity of operation. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies, such as the inability to achieve integrated detection of multiple targets, the increased risk of aerosol cross-contamination due to the opening of amplification products, and the cumbersome operation. Therefore, a One-Pot reaction device is proposed.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A One-Pot reaction apparatus, comprising:

[0007] The reaction tube has a cross-shaped light-blocking plate fixedly installed inside. The reaction tube is divided into four regions by the cross-shaped light-blocking plate. A sealing plate is fixedly installed on the top of the cross-shaped light-blocking plate. Four sample dispensing holes are opened on the top of the sealing plate. Reagent storage capsules are provided on the inner wall of the reaction tube in each of the four regions.

[0008] In one possible design, the four sample dispensing holes correspond to the four regions of the reaction tube, and each of the four sample dispensing holes of the sealing plate is sealed with a sealing film.

[0009] In one possible design, each of the four reagent storage capsules has a connection point fixedly provided on one side of its bottom, and each of the four connection points has a thread fixedly provided on one side, with a breakable section provided in the middle of each of the four threads.

[0010] In one possible design, the cross-shaped light-blocking plate is fixedly provided with multiple evenly distributed needles on one side of each of the four regions.

[0011] In one possible design, both the sealing film and the reagent preservation capsule are made of easily puncturable polyvinyl chloride (PVC).

[0012] In one possible design, the top of the reaction tube is threaded with a sealing screw cap, the outer ring of which has evenly distributed anti-slip stripes.

[0013] In this application, when starting to use the product, first turn the sealing screw cap to remove it from the top of the reaction tube. Then, use a pipette to add the reaction reagent and sample into the reaction tube. First, draw the mixture of reaction reagent and sample into the pipette. Then, pass the pipette tip through the sealing membrane of the sample hole on the sealing plate. Next, push the mixture of reaction reagent and sample into one area of ​​the reaction tube through the pipette tip. Then, follow the above operation to inject the reagent into the other areas of the reaction tube in sequence. After all the reagents in the four areas have been injected, turn the sealing screw cap again to seal the reaction tube and react for 10-20 minutes under the corresponding temperature conditions.

[0014] After the reaction is complete, place the reaction tube in a centrifuge and set the centrifugation speed (low speed) and time (1-2 minutes) according to the experimental requirements. During centrifugation, the centrifugal force drives the reagent preservation capsules to move inside the reaction tube. As the centrifugal force and the movement of the reagent preservation capsules cause the threads to break at their breakable points, causing each reagent preservation capsule to fall into its respective area. The fallen reagent preservation capsules will also move under the action of centrifugal force. During their movement, the reagent preservation capsules will come into contact with the cross-shaped light blocking plate and be punctured by the needles on it, releasing the internal detection reagents into the corresponding areas of the reaction tube. At this time, the four areas contain different detection reagents, realizing multi-target reaction separation.

[0015] This utility model has the following beneficial effects:

[0016] This invention achieves effective separation of multi-target reactions and avoidance of light pollution through the design of a cross-shaped light-blocking plate and a reagent storage capsule. At the same time, it uses centrifugal force to automatically release reagents, making the operation simple and reducing the risk of aerosol contamination caused by frequent opening and closing of the lid. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of a One-Pot reaction device proposed in this utility model;

[0018] Figure 2 This is a front view structural diagram of the reaction tube of a One-Pot reaction device proposed in this utility model;

[0019] Figure 3 This is a side view of the cross-sectional structure of the reaction tube of a One-Pot reaction device proposed in this utility model;

[0020] Figure 4 This is a schematic diagram of the reagent storage capsule and a partially enlarged structure of a One-Pot reaction device proposed in this utility model.

[0021] In the diagram: 1. Reaction tube; 2. Sealing screw cap; 201. Anti-slip stripe; 3. Cross-shaped light-blocking plate; 4. Sealing plate; 5. Sample dispensing hole; 501. Sealing film; 6. Needle; 7. Reagent storage capsule; 8. Connection point; 9. Thread; 901. Easily broken part. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0023] Example 1

[0024] Reference Figure 1-4 A reaction apparatus, comprising:

[0025] The reaction tube 1 has a cross-shaped light-blocking plate 3 fixedly installed inside it. The cross-shaped light-blocking plate 3 divides the reaction tube 1 into four independent areas, so that different target reactions can be carried out in each area, avoiding mutual interference.

[0026] A sealing plate 4 is fixedly installed on the top of the cross-shaped light blocking plate 3. Four sample dispensing holes 5 are opened on the top of the sealing plate 4. These four sample dispensing holes 5 correspond one-to-one with the four areas of the reaction tube 1. In the initial state, each sample dispensing hole 5 is sealed with a sealing film 501, which makes it easy for the pipette tip to penetrate and inject the reagent into the reaction tube 1. The sealing film 501 and the reagent preservation capsule 7 are both made of easily puncturable polyvinyl chloride material, which makes it easy to add samples or puncture the reagent preservation capsule 7 through the pipette tip in the future.

[0027] On the inner wall of reaction tube 1, above each area, there is a reagent storage capsule 7. The reagent storage capsule 7 is used to store the reagents required for the reaction before centrifugation. A connection point 8 is fixedly set on one side of the bottom of each of the four reagent storage capsules 7. A thread 9 is fixedly connected to one side of the connection point 8. A breakable section 901 is set in the middle of the thread 9. The design of this breakable section 901 is to allow the thread 9 to break at the breakable section 901 by the action of centrifugal force during centrifugation, so that the reagent storage capsule 7 falls into the corresponding area of ​​reaction tube 1.

[0028] Each area of ​​the cross-shaped light-blocking plate 3 is fixed with multiple evenly distributed needles 6. When the reagent storage capsule 7 falls into the reaction tube 1, the needles 6 will puncture it, releasing the reagent inside the reagent storage capsule 7 into the corresponding area of ​​the reaction tube 1, thereby starting the reaction.

[0029] This application can be used in the field of One-Pot reaction device technology, or in other fields applicable to this application.

[0030] Example 2

[0031] Based on Embodiment 1, Embodiment 2 further includes: a One-Pot reaction device, which is applied to the field of One-Pot reaction device technology. The top of the reaction tube 1 is also threaded with a sealing screw cap 2. The outer ring of the sealing screw cap 2 is provided with uniformly distributed anti-slip stripes 201. The anti-slip stripes 201 are designed to increase grip stability and facilitate operation when tightening or unscrewing the sealing screw cap 2.

[0032] The accompanying drawings in this application are for illustrative purposes only. The dimensions and shapes of the components shown are not actual limitations but are merely schematic representations. In actual implementation, the components can be reasonably configured and adjusted according to specific needs and actual conditions.

[0033] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A One-Pot reaction apparatus characterized by, Include: Reaction tube (1), the inside of the reaction tube (1) is fixedly provided with a cross-shaped light blocking plate (3), the reaction tube (1) is divided into four areas by the cross-shaped light blocking plate (3), the top of the cross-shaped light blocking plate (3) is fixedly provided with a sealing plate (4), four sample holes (5) are formed in the top of the sealing plate (4), and the inner wall of the reaction tube (1) is provided with a reagent storage bag (7) in the four areas.

2. The One-Pot reaction device according to claim 1, wherein, Four said sample holes (5) correspond to the four areas of the reaction tube (1), and the four sample holes (5) of the sealing plate (4) are sealed with sealing films (501).

3. The One-Pot reaction device of claim 1, wherein, The bottom side of the four reagent storage bags (7) is fixedly provided with a connecting point (8), one side of the four connecting points (8) is fixedly provided with a silk thread (9), and the middle segment of the four silk threads (9) is provided with a breakable part (901).

4. The One-Pot reaction device of claim 1, wherein, The cross-shaped light blocking plate (3) is fixedly provided with a plurality of evenly distributed thorn needles (6) on one side of the four areas.

5. The One-Pot reaction device of claim 2, wherein, The sealing film (501) and the reagent storage bag (7) are both made of easily punctured polyvinyl chloride material.

6. The One-Pot reaction device of claim 1, wherein, The top of the reaction tube (1) is threadedly connected with a sealing screw cap (2), and the outer ring of the sealing screw cap (2) is provided with evenly distributed anti-skid stripes (201).

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