Novel exosome high-purity extraction device

Abstract

The utility model relates to the technical field of exosome purification, and discloses a novel exosome high-purity extraction device which comprises a bottom plate, a tank body is fixedly connected to one side of the top of the bottom plate, a servo motor is fixedly connected to the middle of the top of the tank body, and the output end of the servo motor is fixedly connected with a limiting rod. Hydraulic cylinders are fixedly connected to the two sides of the top of the tank body, fixing rings are fixedly connected to the output ends of the hydraulic cylinders, water tank rings penetrate through and are rotationally connected to the bottoms of the fixing rings, evenly-distributed spray heads penetrate through and are fixedly connected to the inner walls of the water tank rings, and brushes are fixedly connected to the inner walls of the water tank rings. According to the utility model, the servo motor, the limiting rod, the hydraulic cylinder, the fixed ring, the water tank ring, the brush, the limiting shaft, the spray head, the scraper, the hose, the water tank and the water pump are matched, the wall of the tank body is scraped by the scraper, the filter plate is wiped by the brush, and liquid is stirred, so that biological liquid adhesion and filter plate blockage are effectively prevented, and a smooth purification process is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model relates to exosome purification technical field, concretely is a novel exosome high purity extraction device. BACKGROUND

[0002] Exosome is the extracellular vesicle of diameter in 30-150 nanometers, and is released to the extracellular environment after the fusion of intracellular multivesicular body and cell membrane. It is constituted by lipid, protein and nucleic acid etc., and the basic structure is formed by lipid such as phospholipid bilayer, and various proteins such as membrane transport protein and heat shock protein participate in function realization, and exosome is various in function, transmits bioactive molecule to regulate cell behavior in intercellular communication, like tumor exosome transmitting carcinogenic signal, in immune response, the exosome of antigen presenting cell can activate immunity, and also reflects the state of source cell due to component, has great potential in disease diagnosis, and is expected to become important diagnostic marker.

[0003] In the prior art, one way of filtering filter plate is usually to make biological liquid pass through filter plate by using gravity or pressure difference, to make biological liquid flow naturally under the action of self-gravity, or to apply certain pressure at one end of the device to push biological liquid to flow through filter plate, so as to realize solid-liquid separation, make the components meeting the aperture requirements of filter plate pass through filter plate to complete filtration, and larger particle impurities are intercepted on filter plate.

[0004] Lack of automatic cleaning of filter plate and maintenance mechanism of tank body, in the filtering process, biological liquid is easy to adhere to the surface of filter plate and the inside of tank body, leading to filter plate blockage, reducing the filtering efficiency, and also needing frequent shutdown for manual cleaning, affecting the overall work progress, unable to carry out efficient filtration on filter plate, in view of the above problems, therefore, a novel exosome high purity extraction device is provided. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a novel exosome high purity extraction device, and solves the problem of inefficient filtration on filter plate in the background art.

[0006] To achieve the above object, the utility model provides the following technical scheme: A novel exosome high -purity extraction device, including the bottom plate, one side fixed connection of bottom plate top has the jar body, the middle fixed connection of jar body top has the servo motor, the output fixed connection of servo motor has the limiting rod, both sides fixed connection of jar body top has the hydraulic cylinder, the output fixed connection of hydraulic cylinder has the fixed ring, the bottom penetrates and rotationally connects with the water tank ring of fixed ring, the evenly distributed shower head of water tank ring inner wall penetrates and is fixedly connected, the brush of water tank ring inner wall is fixedly connected, one end of brush is fixedly connected with the limiting shaft, and the top of limiting shaft is penetrated with limiting rod and is connected with sliding, the bottom of water tank ring is fixedly connected with the scraper, the outer ring of jar body penetrates and is connected with the pull plate of sliding, the dismounting assembly is arranged in the inner wall of jar body.

[0007] Through the above technical scheme, the driving of the hydraulic cylinder can make the fixed ring move up and down, and the rotation of the water tank ring will not drive the fixed ring to rotate, and the brush can wipe the filter plate or stir the biological liquid.

[0008] As a further description of the above technical scheme: the dismounting assembly includes six springs, the front end and the rear end of the inner wall of the jar body are fixedly connected with the springs, the bottom of the spring is fixedly connected with a fixed block, the bottom of the fixed block is fixedly connected with a fixed rod, and the top of the fixed rod is penetrated with the pull plate and is connected with sliding.

[0009] Through the above technical scheme, the fixed rod can fix the position of the pull plate, and lifting the fixed block can make the fixed rod move.

[0010] As a further description of the above technical scheme: the bottom of the inner wall of the jar body is fixedly connected with a fixed plate.

[0011] Through the above technical scheme, the fixed plate can limit the position of the filter plate.

[0012] As a further description of the above technical scheme: the other side of the top of the bottom plate is fixedly connected with a water tank, and the inner wall of the water tank is fixedly connected with a water pump.

[0013] Through the above technical scheme, the water pump can extract and transport the cleaning liquid in the water tank.

[0014] As a further description of the above technical scheme: the output of the water pump is fixedly connected with a hose, and one end of the hose is penetrated with the fixed ring and is fixedly connected.

[0015] Through the above technical scheme, the hose can stretch according to the up and down movement of the fixed ring.

[0016] As a further description of the above technical solution: the tank top front end is penetrated and fixedly connected with a discharging port, and the tank outer circle one side is penetrated and fixedly connected with a discharging port.

[0017] Through the above technical scheme, the discharging port and the discharging port outer circle are provided with valves.

[0018] As a further description of the above technical solution: the water tank one side is penetrated and slidingly connected with an end cover.

[0019] Through the above technical scheme, the end cover can be pulled out to replace the cleaning liquid in the water tank.

[0020] As a further description of the above technical solution: the pull plate inner wall is fixedly connected with a filter plate, and the filter plate and the fixed block inner wall are penetrated and slidingly connected.

[0021] Through the above technical scheme, the filter plate can filter biological liquid.

[0022] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0023] 1. The novel exosome high-purity extraction device provided by the utility model, through the cooperation between the servo motor, the limiting rod, the hydraulic cylinder, the fixing ring, the water tank ring, the brush, the limiting shaft, the spray head, the scraper, the hose, the water tank and the water pump, through the cooperative operation of multiple components, the scraper scrapes the tank wall, the brush wipes the filter plate and the stirring liquid, effectively prevents the biological liquid from adhering and the filter plate from being blocked, ensures smooth purification process, reduces purification interruption and efficiency reduction caused by blockage, and the combination of the water pump, the water tank, the water tank ring and the spray head can quickly deliver cleaning liquid to the inside of the tank for flushing.

[0024] 2. The novel exosome high-purity extraction device provided by the utility model, through the cooperation between the spring, the fixed block, the fixed rod, the pull plate, the filter plate and the fixed plate, the fixed block can be removed by only being pushed upward, reverse operation can complete installation, without the aid of complex tools or professional skills, ordinary workers can operate after simple training, the convenient and fast disassembly and installation mode enhances the adaptability of the equipment to different biological liquid purification tasks when facing different filtering requirements. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a perspective view of the utility model;

[0026] Figure 2 It is a water tank cutaway perspective view of the utility model;

[0027] Figure 3 It is an exploded view of the fixing ring of the utility model;

[0028] Figure 4 A tank body sectional view of the utility model.

[0029] Legend:

[0030] 1, bottom plate; 2, tank body; 3, servo motor; 4, limit rod; 5, hydraulic cylinder; 6, fixed ring; 7, sink ring; 8, brush; 9, limit shaft; 10, spray head; 11, scraper; 12, hose; 13, water tank; 14, water pump; 15, end cover; 16, spring; 17, fixed block; 18, fixed rod; 19, pull plate; 20, filter plate; 21, fixed plate; 22, discharge opening; 23, discharge opening. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the utility model will be described clearly and completely below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the utility model.

[0032] To further understand the content of the utility model, the utility model will be described in detail with reference to the drawings.

[0033] Reference Figure 1 and Figure 2 The utility model relates to a novel exosome high-purity extraction device, including bottom plate 1, the other side fixedly connected with water tank 13 at bottom plate 1 top, water tank 13 stores cleaning agent inside, is convenient for the washing of tank body 2, water pump 14 is fixedly connected in water tank 13 inner wall, water pump 14 output is fixedly connected with hose 12, and one end of hose 12 is fixedly connected with fixed ring 6 and penetrates, and water pump 14 can transmit cleaning agent to fixed ring 6 inside by hose 12, tank body 2 top front end is fixedly connected with discharge opening 22 and penetrates, and biological liquid enters tank body 2 inside by discharge opening 22, tank body 2 outer circle one side is fixedly connected with discharge opening 23 and penetrates, water tank 13 one side is fixedly connected with end cover 15 and penetrates, pull plate 19 inner wall is fixedly connected with filter plate 20, filter plate 20 can filter biological liquid, and filter plate 20 and fixed block 17 inner wall penetrate and slide connection.

[0034] Reference Figure 2 and Figure 3The bottom plate 1 top side is fixedly connected with a tank body 2, the tank body 2 top middle is fixedly connected with a servo motor 3, the servo motor 3 output end is fixedly connected with a limiting rod 4, the servo motor 3 drives the limiting rod 4 to rotate, the tank body 2 top two sides are fixedly connected with a hydraulic cylinder 5, the hydraulic cylinder 5 output end is fixedly connected with a fixed ring 6, the drive of the hydraulic cylinder 5 makes the fixed ring 6 move up and down, the fixed ring 6 bottom is fixedly connected with a water tank ring 7, the water tank ring 7 can store cleaning fluid, the spray head 10 is convenient to spray, the water tank ring 7 inner wall is fixedly connected with a plurality of spray heads 10, the water tank ring 7 inner wall is fixedly connected with a plurality of brushes 8, the surface of the filter plate 20 can be wiped through the brush 8, and the rotation of the brush 8 can stir the inside of the tank body 2, to prevent the biological liquid from being blocked, one end of the brush 8 is fixedly connected with a limiting shaft 9, and the top of the limiting shaft 9 is fixedly connected with the limiting rod 4, the limiting shaft 9 moves up and down at the limiting rod 4, and the rotation of the limiting rod 4 drives the limiting shaft 9 to rotate, the water tank ring 7 bottom is fixedly connected with a scraper 11, the tank body 2 outer circle is fixedly connected with a pull plate 19, and the tank body 2 inner wall is provided with a disassembly assembly.

[0035] Referring to Figure 1 And Figure 4 The disassembly assembly includes six springs 16, the springs 16 are fixedly connected with the inner wall front end and rear end of the tank body 2, the spring 16 bottom is fixedly connected with a fixed block 17, the spring 16 is compressed and released to reset the fixed block 17, the fixed block 17 bottom is fixedly connected with a fixed rod 18, and the fixed rod 18 is fixedly connected with the top of the pull plate 19, the fixed rod 18 can fix the position of the pull plate 19, and the tank body 2 inner wall bottom is fixedly connected with a fixed plate 21, and the fixed plate 21 can limit the position of the filter plate 20.

[0036] Working principle: first through the biological liquid through the discharge port 22 into the tank 2, then through the filter plate 20 filtration, finally through the discharge port 23 discharge, so as to complete the purification of biological liquid, in the process of filter plate 20 filtration, through the drive of hydraulic cylinder 5 makes fixed ring 6 drive water tank ring 7 up and down, through servo motor 3 drive limit rod 4 produces rotation, limit rod 4 rotation drives limit shaft 9 to produce rotation, limit shaft 9 rotation through the back brush 8 drive water tank ring 7 rotation, can make the scraper 11 to the inner wall of the tank 2 is scraped, prevent biological liquid adhesion, the rotation of brush 8 can wipe the surface of filter plate 20, and can be stirred in the tank 2 biological liquid, prevent clogging, so as to prevent biological liquid adhesion in the inner wall of the tank 2, and can prevent the filter plate 20 clogging, improve the efficiency of biological liquid purification, when the tank 2 inner wall needs to be cleaned, water pump 14 will clean the liquid in water tank 13 through the hose 12 transmission to water tank ring 7, then through the nozzle 10 can flush the inner wall of the tank 2, again through the cooperation between the scraper 11 and brush 8, can clean the inside of the tank 2, when the filter plate 20 needs to be disassembled or replaced, by pushing the fixed block 17 upwards, at the same time, the spring 16 is pressed, the fixed block 17 drives the fixed rod 18 to move upwards, in the process of moving, the fixing of pull plate 19 is released, so that the filter plate 20 can be pulled out and replaced, in the installation of filter plate 20, the fixed block 17 is again actuated, and then the spring 16 is pressed to release, so that the fixed rod 18 enters the fixed block 17, thereby completing the installation of the filter plate 20, which can quickly disassemble the filter plate 20.

[0037] It should be noted that, in the present text, relational terms such as first and second and the like can only be used to distinguish one entity or operation from another entity or operation, without necessarily requiring or implying any such actual relationship or order between these entities or operations. Moreover, the terms "comprises", "comprising", or any other variations thereof are intended to cover non-exclusive inclusions, so that a process, method, article, or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such a process, method, article, or equipment.

[0038] Although the embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A novel high-purity exosome extraction device, comprising a base plate (1), characterized in that: A tank body (2) is fixedly connected to one side of the top of the base plate (1). A servo motor (3) is fixedly connected to the middle of the top of the tank body (2). A limit rod (4) is fixedly connected to the output end of the servo motor (3). Hydraulic cylinders (5) are fixedly connected to both sides of the top of the tank body (2). A fixing ring (6) is fixedly connected to the output end of the hydraulic cylinder (5). A water tank ring (7) is rotatably connected through the bottom of the fixing ring (6). A uniformly distributed nozzle (10) is fixedly connected through the inner wall of the water tank ring (7). A brush (8) is fixedly connected to the inner wall of the water tank ring (7). A limit shaft (9) is fixedly connected to one end of the brush (8). The top of the limit shaft (9) is slidably connected to the limit rod (4). A scraper (11) is fixedly connected to the bottom of the water tank ring (7). A pull plate (19) is slidably connected through the outer ring of the tank body (2). A disassembly assembly is provided on the inner wall of the tank body (2).

2. The novel high-purity exosome extraction device according to claim 1, characterized in that: The disassembly assembly includes six springs (16), which are fixedly connected to the front and rear ends of the inner wall of the tank (2). A fixing block (17) is fixedly connected to the bottom of the spring (16), and a fixing rod (18) is fixedly connected to the bottom of the fixing block (17). The fixing rod (18) is slidably connected to the top of the pull plate (19).

3. The novel high-purity exosome extraction device according to claim 1, characterized in that: The bottom of the inner wall of the tank (2) is fixedly connected to a fixing plate (21).

4. The novel high-purity exosome extraction device according to claim 1, characterized in that: A water tank (13) is fixedly connected to the other side of the top of the base plate (1), and a water pump (14) is fixedly connected to the inner wall of the water tank (13).

5. The novel high-purity exosome extraction device according to claim 4, characterized in that: The output end of the water pump (14) is fixedly connected to a hose (12), and one end of the hose (12) is connected to the fixing ring (6) through and fixedly connected.

6. The novel high-purity exosome extraction device according to claim 1, characterized in that: The top front end of the tank (2) is connected to a discharge port (22), and the outer side of the tank (2) is connected to a discharge port (23).

7. The novel high-purity exosome extraction device according to claim 4, characterized in that: An end cap (15) is slidably connected to one side of the water tank (13).

8. The novel high-purity exosome extraction device according to claim 1, characterized in that: The inner wall of the pull plate (19) is fixedly connected to a filter plate (20), and the filter plate (20) is slidably connected to the inner wall of the fixing block (17).

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