Modularized multilayer breathable tetrahymena thermophila high-throughput culture device

Abstract

The utility model discloses a modular multilayer breathable tetrahymena thermophila high-flux culture device which comprises a box body, a plurality of partition plates are arranged in the box body, the box body is divided into a plurality of culture units by the partition plates, and the interiors of the culture units are jointly connected with an oxygen supply assembly. A top cover is rotationally connected to the top end of each culture unit through a hinge, the middle of each top cover is hollowed out, a breathable film is fixed in each top cover, supporting plates are fixed to the two sides of the inner side wall of each culture unit, and the upper surfaces of the supporting plates are attached to the lower surfaces of the top covers. According to the culture box, the box bodies are arranged, the multiple independent culture units are arranged in the box bodies, the inserting-connecting assemblies and the butt-joint assemblies are arranged at the bottoms and the tops of the box bodies respectively, and when every two adjacent box bodies are stacked up and down, the upper box body and the lower box body are fixed through inserting-connecting of the inserting-connecting assemblies and the butt-joint assemblies; a plurality of stacked box bodies can be conveniently placed on the shaking table at the same time, so that the space of the shaking table is utilized to the maximum extent.

Description

TECHNICAL FIELD

[0001] The utility model relates to tetrahymena thermophila culture technical field especially relates to modularization multilayer breathable tetrahymena thermophila high throughput culture device. BACKGROUND

[0002] Tetrahymena thermophila is the model organism of toxicology research. In order to determine the action concentration of toxic substances on tetrahymena thermophila, multiple concentration gradients from low to high need to be set, and three parallels are made for each concentration. Then the conical flask is placed on the shaker support to fix, and the oscillation culture is carried out. However, the existing tetrahymena thermophila culture device has the following shortcomings:

[0003] In the operation, the large number of conical flasks and the large volume lead to low space utilization in the shaker, and the single culture throughput is limited. The oxygen required for tetrahymena thermophila culture depends on the shaker oscillation to improve the dissolved oxygen supply, which is low in efficiency and limited by the shape of the container.

[0004] Therefore, in view of the above shortcomings, the utility model provides a modularization multilayer breathable tetrahymena thermophila high throughput culture device. UTILITY MODEL CONTENTS

[0005] The utility model aims at solving the shortcomings in the prior art, and provides a modularization multilayer breathable tetrahymena thermophila high throughput culture device.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme: the modularization multilayer breathable tetrahymena thermophila high throughput culture device comprises a box body, a plurality of partitions are arranged in the box body, and the box body is divided into a plurality of culture units by the partitions, a plurality of oxygen supply assemblies are connected in the culture units, a top cover is rotatably connected to the top end of each culture unit through a hinge, the middle part of the top cover is designed as a hollow, a breathable film is fixed in the top cover, support plates are fixed on the two sides of the inner wall of each culture unit, the upper surface of the support plate is attached to the lower surface of the top cover, bottom side plates are fixed on the two sides of the bottom end of the box body, top side plates are fixed on the two sides of the top end of the box body, a plug-in assembly is fixed to the middle part of the lower surface of the bottom side plate, and a docking assembly for plugging into the plug-in assembly is fixed to the middle part of the upper surface of the top side plate.

[0007] Further, support rods are fixed to the lower surface of the bottom side plate.

[0008] Further, the oxygen supply assembly comprises a plurality of branch pipes penetrating through a row of culture units, gas holes are formed on the surface of each culture unit, a main gas pipe is fixedly connected to one end of the plurality of branch pipes outside the box body, and the main gas pipe is connected with a micro air pump.

[0009] Further, a one-way valve and a microporous filter membrane are fixed to the middle part of the main gas pipe.

[0010] Further, the upper surface of the top cover away from the hinge side is fixed with a handle, and the lower surface of the top cover and the upper surface of the support plate are both fixed with a magnet.

[0011] Further, the side edge of the top cover is provided with an annular groove, and a sealing strip is fixedly connected in the annular groove.

[0012] Further, the plug-in assembly comprises a plug rod fixed to the lower surface of the bottom side plate, a groove is formed in the side wall of the bottom end of the plug rod, a spring is fixedly connected to the inner side wall of the groove, and a clamping tooth is fixedly connected to the other end of the spring, and the butt joint assembly comprises a clamping sleeve fixed to the upper surface of the top side plate, and a clamping hole is formed in the side wall of the side close to the clamping tooth of the clamping sleeve.

[0013] The utility model discloses the beneficial effect that:

[0014] 1. When using, the modular multi-layer breathable Tetrahymena thermophila high-throughput culture device is provided with a box body, a plurality of separate culture units are arranged in the box body, plug-in assemblies and butt joint assemblies are arranged at the bottom and top of the box body respectively, when two adjacent box bodies are placed in a stacked mode, the plug-in assemblies and the butt joint assemblies are plugged in to fix the two box bodies, the stacked box bodies can be placed on a shaking table at the same time, and the space of the shaking table is utilized to the maximum.

[0015] 2. When using, the modular multi-layer breathable Tetrahymena thermophila high-throughput culture device is provided with a box body, a top cover and a breathable membrane are arranged at the top of each culture unit, and an oxygen supply assembly is connected to the plurality of units of each box body, the breathable membrane allows gas exchange but blocks microbial contamination, then clean oxygen is provided to each culture unit through the oxygen supply assembly, the oxygen supply mode is optimized, and the dependence on mechanical oscillation is reduced. In addition, since the top cover is arranged at the top of each culture unit, each culture unit can be sampled individually, and the pollution of other culture units during liquid addition and sampling is avoided. DRAWINGS

[0016] In order to more clearly illustrate the technical scheme of the utility model, the following will be a brief introduction to the drawings needed to be used in the specific implementation mode, obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to these drawings without creating labor.

[0017] Figure 1 The utility model discloses a perspective view;

[0018] Figure 2 The utility model discloses a stacked placement section view;

[0019] Figure 3 The utility model discloses aFigure 2 Enlarged view at A;

[0020] Figure 4 The utility model discloses Figure 2 Enlarged view at B.

[0021] Reference signs are as follows:

[0022] 1, box body;101, bottom side plate;102, top side plate;2, culture unit;3, oxygen supply assembly;31, branch pipe;32, main gas pipe;33, one-way valve;34, microporous filter membrane;4, top cover;41, sealing strip;5, gas permeable membrane;6, support plate;7, plug-in assembly;71, plug rod;72, groove;73, spring;74, clamping tooth;8, docking assembly;81, clamping sleeve;82, clamping hole;9, support rod. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] As Figures 1-4 shown, relate to a kind of modular multilayer breathable T. thermophila high-throughput culture device, including box body 1, multiple partitions are arranged in box body 1 interior, and box body 1 is divided into several culture units 2 by partition, and several culture units 2 interior are connected with oxygen supply assembly 3, each culture unit 2 top end is rotatably connected with top cover 4 by hinge, and the middle part of top cover 4 is hollow design, and gas permeable membrane 5 is fixed in top cover 4 interior, the both sides of each culture unit 2 inner wall are fixed with support plate 6, and the upper surface of support plate 6 and the lower surface of top cover 4 are pasted, the bottom end of box body 1 both sides are fixed with bottom side plate 101, and the top end of box body 1 both sides are fixed with top side plate 102, the lower surface middle part of bottom side plate 101 is fixed with plug-in assembly 7, and the upper surface middle part of top side plate 102 is fixed with docking assembly 8 for clamping into plug-in assembly 7.

[0025] In the embodiment, box body 1 and the partition inside box body 1 adopt polycarbonate (PC) or medical grade polypropylene (PP), and can withstand high temperature and high pressure sterilization.

[0026] The lower surface both ends of bottom side plate 101 are fixed with support rod 9.

[0027] By being provided with support rod 9, box body 1 can be conveniently supported.

[0028] The oxygen supply assembly 3 comprises a plurality of branch pipes 31 penetrating through the plurality of culture units 2 in a row, and the branch pipes 31 are provided with air holes on the surface of each culture unit 2. The plurality of branch pipes 31 are fixedly connected with a main air pipe 32 at one end outside the box body 1, and the main air pipe 32 is connected with a micro air pump.

[0029] In this embodiment, the main air pipe 32 is connected with the air outlet of the micro air pump through a hose. The micro air pump pumps air into the main air pipe 32, and the air is then distributed to each branch pipe 31, and finally discharged from each air hole on the branch pipe 31, thereby providing air and oxygen for each culture unit 2.

[0030] The gas permeable membrane 5 is made of PDMS or PTFE material, which allows gas exchange but blocks microbial contamination. The air introduced into each culture unit 2 can pass through the gas permeable membrane 5 and be discharged.

[0031] The main air pipe 32 is fixedly provided with a one-way valve 33 and a microporous filter membrane 34 in the middle.

[0032] When air is introduced into the branch pipe 31 through the main air pipe 32, the one-way valve 33 prevents the culture medium in the culture unit 2 from being discharged along the branch pipe 31, and sterile air or oxygen is injected into the culture medium through the microporous filter membrane 34, thereby meeting the oxygen demand in the cultivation of Tetrahymena thermophila.

[0033] A handle is fixed to the upper surface of the top cover 4 away from the hinge. Magnets are fixed to the lower surface of the top cover 4 and the upper surface of the support plate 6.

[0034] The handle facilitates the rotation of the top cover 4. When the top cover 4 is closed and attached to the support plate 6, the magnets attract and fix the top cover 4.

[0035] A ring-shaped groove is formed in the side edge of the top cover 4, and a sealing strip 41 is fixedly connected inside the ring-shaped groove.

[0036] When the top cover 4 is closed on the surface of the support plate 6, the sealing strip 41 starts the sealing function, preventing the entry of external unclean air into the culture unit 2 through the gap in the side edge of the top cover 4.

[0037] The plug-in assembly 7 comprises a plug rod 71 fixed to the lower surface of the bottom side plate 101. A groove 72 is formed in the bottom end side wall of the plug rod 71, and a spring 73 is fixedly connected to the inner side wall of the groove 72. The other end of the spring 73 is fixedly connected with a clamping tooth 74. The docking assembly 8 comprises a clamping sleeve 81 fixed to the upper surface of the top side plate 102, and a clamping hole 82 is formed in the side wall of the clamping sleeve 81 close to the clamping tooth 74.

[0038] In the embodiment, the top of the tooth 74 away from the end of the spring 73 is designed as an inclined surface, so when the upper and lower box bodies 1 are fixed by the plug-in assembly 7 and the docking assembly 8, the bottom end of the plug rod 71 of the upper box body 1 is inserted into the inside of the sleeve 81 of the lower box body 1, the tooth 74 is first pressed into the groove 72 by the inner wall of the sleeve 81, when the tooth 74 is coincided with the position of the clamping hole 82, the spring 73 presses the tooth 74 into the clamping hole 82, realizing the fixed connection between the plug rod 71 and the sleeve 81, and realizing the stacking and fixing of the upper and lower box bodies 1. When disassembling, the tooth 74 is first pressed to separate from the clamping hole 82, and then the plug rod 71 can be pulled out from the inside of the sleeve 81.

[0039] Working principle: when in use, each top cover 4 is opened by rotating alone, then the culture medium and tetrahymena thermophila are added in the culture unit 2, then the top cover 4 is covered, when the culture unit 2 of each box body 1 is added, the multiple box bodies 1 are placed in a stacking mode, the upper and lower box bodies 1 are fixed and connected through the plug-in assembly 7 and the docking assembly 8, finally the stacked multiple box bodies 1 are placed on a shaking table, each box body 1 is connected with a micro air pump through the main air pipe 32, and clean oxygen can be provided for the culture unit 2 of each box body 1 through the micro air pump.

[0040] The preferred embodiments disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details and limit the utility model to the specific implementation. Obviously, according to the content of the specification, many modifications and changes can be made. The specification selects and specifically describes these embodiments, in order to better explain the principle and practical application of the utility model, so that the person skilled in the art can well understand and utilize the utility model. The utility model is limited by the claims and the whole scope and equivalents.

Claims

1. A modular multilayer air-lifted Tetrahymena thermophila high-throughput cultivation device, characterized in that: The utility model provides a culture box, including box (1), a plurality of partitions are arranged inside the box (1), and the box (1) is divided into several culture units (2) by the partition, a plurality of oxygen supply assemblies (3) are connected in the culture unit (2) together, the top of each culture unit (2) is rotatably connected with the top cover (4) through the hinge, and the middle part of top cover (4) is hollow design, the breathable film (5) is fixed in the top cover (4), the both sides of the inner wall of each culture unit (2) are fixed with the support plate (6), and the upper surface of support plate (6) is bonded with the lower surface of top cover (4), the bottom of the both sides of box (1) is fixed with the bottom side plate (101), and the both sides of the top of box (1) are fixed with the top side plate (102), the lower surface middle part of bottom side plate (101) is fixed with the plug-in assembly (7), the upper surface middle part of top side plate (102) is fixed with the docking assembly (8) for clamping into plug-in assembly (7).

2. The modular multilayer Tetrahymena high throughput culture device of claim 1, wherein: The lower surface both ends of bottom side plate (101) are fixed with support rod (9).

3. The modular multilayer Tetrahymena high throughput culture device of claim 1, wherein: The oxygen supply assembly (3) includes a plurality of branch pipes (31) penetrating through a row of culture units (2), and the branch pipe (31) is provided with a gas hole on the surface of each culture unit (2), and the plurality of branch pipes (31) are fixedly connected with a main gas pipe (32) at one end outside the box (1), and the main gas pipe (32) is connected with a micro air pump.

4. The modular multilayer Tetrahymena high throughput culture device of claim 3, wherein: The main gas pipe (32) is fixed with a one-way valve (33) and a microporous filter membrane (34) in the middle part.

5. The modular multilayer Tetrahymena high throughput culture device of claim 1, wherein: The upper surface of the side, away from the hinge, of the top cover (4) is fixed with a handle, and the lower surface of the top cover (4) and the upper surface of the support plate (6) are both fixed with a magnet.

6. The modular multilayer Tetrahymena high throughput culture device of claim 1, wherein: The side of the top cover (4) is provided with an annular groove, and a sealing strip (41) is fixedly connected in the annular groove.

7. The modular multilayer Tetrahymena high throughput culture device of claim 1, wherein: The plug-in assembly (7) includes a plug rod (71) fixed to the lower surface of the bottom side plate (101), the bottom end of the plug rod (71) is provided with a groove (72) on the side wall, and a spring (73) is fixedly connected to the inner side wall of the groove (72), the other end of the spring (73) is fixedly connected with a clamping tooth (74), and the docking assembly (8) includes a clamping sleeve (81) fixed to the upper surface of the top side plate (102), and a clamping hole (82) is formed in the side wall of the clamping sleeve (81) close to the clamping tooth (74).

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