Semi-automatic culture instrument for cell culture

Abstract

The utility model belongs to the field of cell culture, and particularly relates to a semi-automatic culture instrument for cell culture, which comprises a frame body, a culture tube is inserted into the top end of the frame body, a bump is fixedly connected to the rear side of the top end of the culture tube, and a sealing component is arranged on the rear side of the top end of the frame body. After the culture tubes are placed in the corresponding positions of the frame body, the storage plates can drive the cover plates to cover the tops of the culture tubes, the positions, covering the culture tubes, of the storage plates are accurate and do not have errors, operators do not need to manually cover the cover plates again, and therefore on the premise that the internal sealing performance and safety of the culture tubes are improved, the culture tubes can be conveniently and rapidly stored. The operation amount of an operator in batch operation is also reduced; an operator can directly press the control switch to enable the culture tube to be slowly pushed upwards, and at the moment, the operator can easily take down the culture tube, so that the situation that cells in the culture tube are damaged or die due to the fact that the movement amplitude is too large when the culture tube is pulled out too quickly is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture field, concretely is a kind of semi-automatic culture instrument for cell culture. BACKGROUND

[0002] In the treatment of cancer and other diseases, NK cells are separated, expanded and activated to enhance their anticancer ability, while CAR-T cells are genetically engineered to express specific CARs to enhance their specific anticancer ability, so NK cell and CAR-T cell culture are two important immune cell therapies, and in the process of cultivating, growing and maintaining cells, culture instruments are needed to culture cells, so that cells can obtain better culture environment, avoid external factors such as temperature, humidity, gas concentration and nutrient supply affecting cell growth, so as to ensure that cells remain active and functional during in vitro culture, and common cell culture instruments include culture dishes and culture tubes.

[0003] Currently, when using culture tubes to cultivate cells, the lid needs to be manually covered after the cells are placed in the culture tube, which will result in low efficiency if batch operation is required, and manual operation may result in inconsistent tightness of the lid, affecting the sealing of the culture tube and the stability of the cell culture environment, and in the process of removing the culture tube, if the culture tube is manually removed from the inside of the test tube rack, and the culture tube is stuck in the inside of the test tube rack, the operator may cause the cells inside to move significantly under the action of inertia when forcibly removing the culture tube, which may damage or kill the cells due to mechanical shear force; Therefore, a semi-automatic culture instrument for cell culture is proposed to solve the above problems. SUMMARY

[0004] To make up for the shortcomings of the prior art and avoid errors in the stability and safety of cultured cells, the utility model provides a semi-automatic culture instrument for cell culture.

[0005] The utility model solves the technical problems by adopting the technical scheme of a semi-automatic culture instrument for cell culture, which comprises a frame body, a culture tube is inserted into the inside of the top end of the frame body, a protruding block is fixedly connected to the rear side of the top end of the culture tube, a sealing assembly is arranged on the rear side of the top end of the frame body, and a boosting assembly is arranged on the bottom wall of the frame body.

[0006] The sealing assembly comprises a fixed table fixedly connected to the rear side of the top end of the frame body, a rotating rod rotatably connected to the rear side of the fixed table, a connecting plate fixedly connected to the surface of the rotating rod, a storage plate fixedly connected to the rear end of the connecting plate, a cavity formed in the top end of the storage plate, a cover plate detachably connected to the cavity, and a handle fixedly connected to the rear side of the cover plate.

[0007] Preferably, the rear side of the handle extends to the outside of the cavity, and a clamping groove is formed in the bottom end of the storage plate and matches the clamping of the clamping block, so as to limit the cover plate inside the storage plate, the top end of the cover plate matches the top end of the culture tube, and the culture tube is sealed by rotating the storage plate to drive the cover plate to cover the top end of the culture tube.

[0008] Preferably, a sliding rod is slidably connected to the inside of the fixed table, an extension plate is fixedly connected to the top surface of the sliding rod, a positioning rod is fixedly connected to the inner wall of the fixed table, a rack is slidably connected to the surface of the positioning rod, and a gear is fixedly connected to the surface of the connecting plate.

[0009] Preferably, the top end of the sliding rod matches the bottom end of the protrusion, the bottom end of the sliding rod penetrates through the top wall of the fixed table and the frame body, the rack and the gear are meshingly connected, the rack is fixedly connected to the rear end of the extension plate, and when the culture tube drives the protrusion to move downward, the extension plate is pushed to move, at this time, the extension plate pulls the rack to move and drives the meshing gear to rotate, so as to drive the storage plate to rotate and make the cover plate cover the top of the culture tube.

[0010] Preferably, the boosting assembly comprises a base fixedly connected to the bottom wall of the frame body, an electromagnet fixedly connected to the top end of the base, a guide rod fixedly connected to the top end of the base, a lifting plate arranged above the base, a side plate fixedly connected to the surface of the lifting plate, a magnetic block fixedly connected to the surface of the bottom of the lifting plate, support rods fixedly connected to the front and rear sides of the bottom wall of the frame body, a buffer spring fixedly connected to the top wall of the support rod, and a buffer plate fixedly connected to the bottom end of the buffer spring.

[0011] Preferably, the electromagnet is electrically connected to a control switch, the electromagnet and the magnetic block have the same magnetic pole at the proximal end, and the side plate is slidably connected to the surface of the guide rod, so that when the control switch is operated to make the electromagnet electrified, the magnetic block is repelled, and the magnetic block drives the lifting plate to move upward.

[0012] As preferred, the bottom end of the buffer plate and the top end of the magnetic block are in contact, the bottom end of the culture tube is in contact with the inside of the top end of the lifting plate, and the lifting plate lowers the instantaneous speed under the buffering of the buffer spring when moving upward, avoiding the culture tube being pushed by the lifting plate to cause the cells inside the culture tube to have a large displacement, thereby protecting the safety of the cells inside the culture tube.

[0013] The utility model discloses the beneficial effect lies in:

[0014] The utility model discloses the culture tube is put into the corresponding position of frame body, and the storage plate covers the top of culture tube at this moment, not only the position of culture tube covered by the storage plate is accurate and does not have error, and the operating personnel need not manually cover the cover plate again, thereby improving the internal sealing property and the safety of culture tube, and reducing the operation amount of operating personnel in batch operation under the premise.

[0015] The operating personnel can directly press the control switch to slowly push the culture tube upward, and the operating personnel can easily take down the culture tube, avoiding the culture tube being pulled out too fast to cause the cells inside the culture tube to be damaged or die due to too large displacement. DRAWINGS

[0016] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0017] Figure 1 It is the three-dimensional structure schematic diagram of the utility model;

[0018] Figure 2 It is the bottom structure schematic diagram of the sealing assembly of the utility model;

[0019] Figure 3 It is the surface structure schematic diagram of the storage plate of the utility model;

[0020] Figure 4 It is the Figure 3 It is the enlarged structure schematic diagram of A in the utility model;

[0021] Figure 5 It is the structure schematic diagram of the rack installation of the utility model;

[0022] Figure 6 It is the structure schematic diagram of the boost assembly of the utility model.

[0023] In the figure: 1, frame body; 21, culture tube; 22, protruding block; 3, sealing assembly; 31, fixed table; 321, rotating rod; 322, connecting plate; 331, storage plate; 332, cavity; 333, cover plate; 334, handle; 335, clamping block; 341, sliding rod; 342, extension plate; 343, positioning rod; 344, rack; 345, gear; 4, boosting assembly; 41, base; 42, electromagnet; 43, guide rod; 441, lifting plate; 442, side plate; 45, magnetic block; 461, supporting rod; 462, buffer spring; 463, buffer plate. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0025] The drawings will be described below Figures 1-6 The application will be further described in detail,

[0026] The embodiment of the application discloses a kind of semi-automatic culture instruments for cell culture. Refer to Figure 1 A kind of semi-automatic culture instruments for cell culture, including frame body 1, the inside of the top end of frame body 1 is inserted with culture tube 21, the rear side of the top end of culture tube 21 is fixedly connected with protruding block 22, the rear side of the top end of frame body 1 is provided with sealing assembly 3, the bottom wall of frame body 1 is provided with boosting assembly 4;

[0027] Refer to Figures 2-4 Sealing assembly 3 includes fixedly connected to the rear side of the top end of frame body 1 fixed table 31, the rear side of fixed table 31 is rotatably connected with rotating rod 321, the surface of rotating rod 321 is fixedly connected with connecting plate 322, the rear end of connecting plate 322 is fixedly connected with storage plate 331, the inside of the top end of storage plate 331 is provided with cavity 332, the inside of cavity 332 is clamped with cover plate 333, the rear side of cover plate 333 is fixedly connected with handle 334, the bottom end of handle 334 is fixedly connected with clamping block 335, the rear side of handle 334 extends to the outside of cavity 332, the bottom end of storage plate 331 is provided with clamping groove matched with clamping block 335, so as to limit cover plate 333 in the inside of storage plate 331, the top end of cover plate 333 is matched with the top end of culture tube 21, rotating storage plate 331 drives cover plate 333 to rotate and cover the top end of culture tube 21, so as to seal culture tube 21.

[0028] Refer to Figures 4-5The inner slide connection of the fixed table 31 is provided with a sliding rod 341, the surface of the top of the sliding rod 341 is fixedly connected with an extension plate 342, the inner wall of the fixed table 31 is fixedly connected with a positioning rod 343, the surface of the positioning rod 343 is slidably connected with a rack 344, the surface of the connecting plate 322 is fixedly connected with a gear 345, the top end of the sliding rod 341 and the bottom end of the protrusion 22 are in contact, the bottom end of the sliding rod 341 penetrates through the top wall of the fixed table 31 and the frame body 1, the rack 344 and the gear 345 are in meshing connection, the rack 344 is fixedly connected to the rear end of the extension plate 342, when the culture tube 21 drives the protrusion 22 to move downward, the extension plate 342 is pushed to move, at this time, the extension plate 342 pulls the rack 344 to move, thereby driving the meshing gear 345 to rotate, so as to drive the storage plate 331 to rotate and make the cover plate 333 cover the top of the culture tube 21.

[0029] Referring to Figure 6 The boost assembly 4 comprises a base 41 fixedly connected to the bottom wall of the frame body 1, the top end of the base 41 is fixedly connected with an electromagnet 42, the top end of the base 41 is fixedly connected with a guide rod 43, the upper side of the base 41 is provided with a lifting plate 441, the surface of the lifting plate 441 is fixedly connected with a side plate 442, the surface of the bottom of the lifting plate 441 is fixedly connected with a magnetic block 45, the electromagnet 42 is electrically connected with a control switch, the proximal ends of the electromagnet 42 and the magnetic block 45 are of the same magnetic pole, the side plate 442 is slidably connected to the surface of the guide rod 43, when the control switch is operated to make the electromagnet 42 electrified, the magnetic block 45 is repelled, so that the magnetic block 45 drives the lifting plate 441 to move upward, the front and rear sides of the bottom wall of the frame body 1 are fixedly connected with support rods 461, the top wall of the support rod 461 is fixedly connected with a buffer spring 462, the bottom end of the buffer spring 462 is fixedly connected with a buffer plate 463, the bottom end of the buffer plate 463 and the top end of the magnetic block 45 are in contact, the bottom end of the culture tube 21 is telescopically connected in the inside of the top end of the lifting plate 441, when the lifting plate 441 moves upward, the buffer spring 462 reduces the instantaneous speed, avoiding the lifting plate 441 pushing the culture tube 21 too fast to cause the cells in the culture tube 21 to move greatly, thereby protecting the safety of the cells in the culture tube 21.

[0030] Working principle: the operator first holds the handle 334 and pushes the clean cover plate 333 into the inside of the storage plate 331, at this time the clamping block 335 is clamped in the inside of the clamping groove, then the culture tube 21 is inserted into the corresponding position of the frame body 1, at this time the culture tube 21 drives the protruding block 22 to move downward, and the protruding block 22 pushes the sliding rod 341 downward, and the sliding rod 341 drives the extension plate 342 to move downward, at the same time the extension plate 342 pulls the rack 344, the rack 344 is always engaged with the gear 345 under the guidance of the positioning rod 343, so that the rack 344 is pulled to drive the engaged gear 345 to rotate, at this time the gear 345 drives the fixed connecting plate 322 to rotate, and the connecting plate 322 drives the storage plate 331 to rotate, at this time the storage plate 331 drives the inside cover plate 333 to rotate to the culture tube 21, until the cover plate 333 rotates and covers the top end of the culture tube 21, and at this time the culture tube 21 is completely inserted into the inside of the frame body 1, and the bottom of the culture tube 21 is in contact with the inner wall at the top end of the lifting plate 441.

[0031] Then the operator needs to take down the culture tube 21, at this time the control switch is pressed first, the control switch makes the electromagnet 42 electrified, and the electromagnet 42 repels the magnetic block 45 with the same magnetic pole, at this time the magnetic block 45 is pushed by the repulsive force and drives the lifting plate 441 to move upward, and the lifting plate 441 and the magnetic block 45 move upward and contact the buffer plate 463 and compress the buffer spring 462, at this time the lifting plate 441 moves upward at a reduced speed, so that the lifting plate 441 drives the culture tube 21 to move upward slowly, so that the culture tube 21 is slowly boosted upward, then the operator can easily take down the culture tube 21 from the inside of the frame body 1.

[0032] The basic principle, main characteristics and advantages of the present application are shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application.

Claims

1. A semi-automatic culture apparatus for cell culture, characterized by: The utility model provides a culture tube sealing device, including frame (1), the inside plug -in of frame (1) top end is cultured pipe (21), the rear side fixed connection of cultured pipe (21) top end is protruding block (22), the rear side of frame (1) top end is provided with sealing assembly (3), the bottom wall of frame (1) is provided with boost assembly (4); The sealing assembly (3) includes a fixed table (31) fixedly connected to the rear side of the top end of the frame (1), a rotating rod (321) rotatably connected to the rear side of the fixed table (31), a connecting plate (322) fixedly connected to the surface of the rotating rod (321), a storage plate (331) fixedly connected to the rear end of the connecting plate (322), a cavity (332) formed in the top end of the storage plate (331), a cover plate (333) inserted into the cavity (332), a handle (334) fixedly connected to the rear side of the cover plate (333), and a clamping block (335) fixedly connected to the bottom end of the handle (334).

2. A semi-automatic culture apparatus for cell culture according to claim 1, characterized in that: The rear side of the handle (334) extends to the outside of the cavity (332), the bottom end of the storage plate (331) is provided with a clamping groove matched with the clamping block (335), and the top end of the cover plate (333) is matched with the top end of the culture tube (21).

3. A semi-automatic culture apparatus for cell culture according to claim 1, characterized in that: The inside of the fixed table (31) is slidably connected with a sliding rod (341), the top surface of the sliding rod (341) is fixedly connected with an extension plate (342), the inner wall of the fixed table (31) is fixedly connected with a positioning rod (343), the surface of the positioning rod (343) is slidably connected with a rack (344), and the surface of the connecting plate (322) is fixedly connected with a gear (345).

4. A semi-automatic culture apparatus for cell culture according to claim 3, characterized in that: The top end of the sliding rod (341) is matched with the bottom end of the protruding block (22), the bottom end of the sliding rod (341) penetrates through the top wall of the fixed table (31) and the frame (1), the rack (344) is meshingly connected with the gear (345), and the rack (344) is fixedly connected to the rear end of the extension plate (342).

5. The semi-automatic culture apparatus for cell culture according to claim 1, characterized in that: The boost assembly (4) includes a base (41) fixedly connected to the bottom wall of the frame (1), an electromagnet (42) fixedly connected to the top end of the base (41), a guide rod (43) fixedly connected to the top end of the base (41), a lifting plate (441) arranged above the base (41), a side plate (442) fixedly connected to the surface of the lifting plate (441), a magnetic block (45) fixedly connected to the surface of the bottom of the lifting plate (441), support rods (461) fixedly connected to the front and rear sides of the bottom wall of the frame (1), buffer springs (462) fixedly connected to the top wall of the support rods (461), and buffer plates (463) fixedly connected to the bottom end of the buffer springs (462).

6. A semi-automatic culture apparatus for cell culture according to claim 5, characterized in that: The electromagnet (42) is electrically connected with a control switch, the proximal ends of the electromagnet (42) and the magnetic block (45) have the same magnetic pole, and the side plate (442) is slidably connected to the surface of the guide rod (43).

7. A semi-automatic culture apparatus for cell culture according to claim 5, characterized in that: The bottom end of the buffer plate (463) and the top end of the magnetic block (45) are in contact, and the bottom end of the culture tube (21) is in contact with the inside of the top end of the lifting plate (441).

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