Waterproof buoy for water bath resuscitation of cells
By designing the sleeve and timing module of the water-resistant float, the problem of cell contamination during water bath resuscitation was solved, achieving low-cost and high-efficiency cell resuscitation, which is suitable for cell water bath resuscitation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANGHAI ANGECON BIOTECH
- Filing Date
- 2025-02-26
- Publication Date
- 2026-04-17
AI Technical Summary
Traditional water bath resuscitation methods are prone to cell contamination, and waterless resuscitation devices are expensive and have poor resuscitation effects, especially for sensitive and fragile cells, which current technologies cannot effectively solve.
A water-resistant float for cell resuscitation in a water bath is designed. By setting a sleeve and a sealed storage cavity on the float body, the cell cryopreservation container is prevented from directly contacting the liquid medium. Combined with an adjustable number of sleeves and a timing module, water-resistant heating is achieved.
Without affecting heat conduction, cell contamination is avoided, ensuring resuscitation results are similar to those of traditional water baths, and at a lower cost than anhydrous resuscitation devices.
Smart Images
Figure CN224133069U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell resuscitation technology, and in particular to a water-resistant float for cell water bath resuscitation. Background Technology
[0002] Cell resuscitation is the opposite of cell cryopreservation, that is, the process of cell recovery and growth. It involves thawing cells frozen in liquid nitrogen or a -70°C freezer and then reculturing them. Traditional cell resuscitation methods usually use a water bath. Specifically, the cryovials or cryopreservation bags are taken out of the liquid nitrogen container and directly immersed in a 37°C water bath, and shaken from time to time to thaw them as quickly as possible. Alternatively, an anhydrous resuscitation device can be used for treatment.
[0003] However, the aforementioned existing technology has the following drawbacks:
[0004] The warm and humid environment inside a water bath is prone to bacterial growth, which can easily lead to contamination of the revived cell samples during the thawing process if the cells come into direct contact with the cryovials. While anhydrous resuscitation systems have largely solved the problem of introducing contaminants into water baths, their thawing effect is still inferior to that of water baths for relatively sensitive and fragile cells. Furthermore, anhydrous resuscitation systems are more expensive.
[0005] Therefore, there is an urgent need for a water-resistant float for cell resuscitation that can prevent cell cryopreservation containers from directly contacting the liquid medium and introducing contaminants while ensuring the effectiveness of water bath resuscitation. Utility Model Content
[0006] To solve the above-mentioned technical problems, this utility model provides a water-resistant float for cell water bath resuscitation.
[0007] According to one objective of this utility model, this utility model provides a water-resistant float for cell water bath resuscitation, including a float body, wherein a floating direction is provided on the float body, and the two sides of the floating direction are the water bath side and the exposure side, respectively.
[0008] The float body is provided with a sleeve extending toward the water bath side, and the sleeve is provided with a storage cavity that matches the cell cryopreservation container. The float body and the sleeve are sealed and detachable to open and close the storage cavity of the sleeve, and the number of sleeves is adjustable.
[0009] Preferably, the float body includes an external float frame and a built-in float adapter, the built-in float adapter being provided with the sleeve, and the built-in float adapter being replaceably installed on the external float frame.
[0010] Preferably, the center of the float body extends toward the exposed side to form a handle, and the handle is detachably connected to the exposed side of the external float frame.
[0011] Preferably, the handle is columnar, and the radial outer side of the handle is arc-shaped, wherein in the floating direction, the radial dimension of the handle gradually decreases away from the float body.
[0012] Preferably, the hand handle is provided with anti-slip particles.
[0013] Preferably, the external float frame adopts a cylindrical structure with the same axial direction and floating direction. The built-in float adapter is disc-shaped. The inner side of the water bath side of the external float frame and the outer side of the built-in float adapter are threaded together. The handle includes a connecting part and a handle part. The connecting part is hollow columnar. The water bath side of the connecting part is provided with an opening. The inner side of the opening is threaded together with the outer side of the external float frame. The center of the exposed side of the connecting part extends outward along the floating direction to form the handle. The connecting part, the built-in float adapter, and the external float frame form a floating cavity. The floating cavity and the storage cavity are connected.
[0014] Preferably, the water bath side of the outer float frame extends radially outward to form an abutment portion, the radial outer side of the abutment portion is flush with the radial outer side of the connecting portion, and the exposed side of the abutment portion and the water bath side of the connecting portion are sealed and matched.
[0015] Preferably, the sleeves are evenly distributed on the water bath side of the float body.
[0016] Preferably, the water-resistant float is provided with a timing module, which is configured to start timing when the water-resistant float comes into contact with the liquid medium, and the timing module is located inside the hand handle.
[0017] Preferably, the hand handle is provided with adjustment buttons, including a start / stop button, a minute setting button, and a second setting button, and the hand handle is provided with a display screen that is electrically connected to the timing module.
[0018] Compared with the prior art, the beneficial effects of this utility model are:
[0019] This water-resistant float for cell resuscitation allows cell cryopreservation containers to be placed inside the storage cavity of a sleeve. The sleeve, together with the float body, seals the cell cryopreservation containers. The float body is then placed in the liquid medium for water resuscitation, with the sleeve completely submerged below the surface of the liquid medium. This achieves water-resistant heating of the cell cryopreservation containers, preventing the cell cryopreservation containers from directly contacting the liquid medium and introducing contaminants.
[0020] The number of cannulas can be adjusted according to the requirements of different batch recovery to meet the requirements of different batch recovery.
[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments. Attached Figure Description
[0022] Figure 1 This is a partial cross-sectional schematic diagram of the overall unfolded structure of the water-resistant float for cell resuscitation described in this utility model;
[0023] Figure 2 This is a partial cross-sectional schematic diagram of the water-resistant float for cell resuscitation described in this utility model;
[0024] Figure 3 This is a schematic diagram of the overall unfolded structure of the water-resistant float for cell resuscitation described in this utility model.
[0025] Figure 4 This is a schematic diagram showing the connection between the sleeve and the float adapter of a water-resistant float for cell resuscitation described in this utility model. Detailed Implementation
[0026] The following description is intended to provide a detailed account of the present invention so that those skilled in the art can implement it. The preferred embodiments described below are merely examples, and other obvious variations will occur to those skilled in the art. The basic principles of the present invention defined in the following description can be applied to other embodiments, modifications, improvements, equivalents, and other technical solutions that do not depart from the spirit and scope of the present invention.
[0027] Please see Figure 1-3 This utility model provides a technical solution: a water-resistant float for cell water bath resuscitation, including a float body 100, wherein a floating direction is provided on the float body 100, and the two sides of the floating direction are the water bath side a and the exposure side b, respectively.
[0028] The float body 100 is provided with a sleeve 200 extending toward the water bath side a. The sleeve 200 is provided with a storage cavity 200a that matches the cell cryopreservation container. The float body 100 and the sleeve 200 are sealed and detachably connected to open and close the storage cavity 200a of the sleeve 200. The number of sleeves 200 is adjustable.
[0029] In use, the cell cryopreservation container can be placed in the storage cavity 200a of the sleeve 200. The sleeve 200, together with the float body 100, seals the cell cryopreservation container. The float body 100 is then placed in the liquid medium for water bath resuscitation, wherein the sleeve 200 is completely submerged below the liquid surface of the liquid medium to achieve water-isolated heating of the cell cryopreservation container and avoid the cell cryopreservation container from directly contacting the liquid medium and introducing contaminants.
[0030] The number of 200 sheaths can be adjusted according to the requirements of different batch recovery to meet the requirements of different batch recovery.
[0031] In one embodiment of this utility model, the liquid medium used for water bath resuscitation is an aqueous solution at 37°C.
[0032] Regarding the specific structure of the floating body, the float body 100 includes an outer float frame 101 and a built-in float adapter 102. The built-in float adapter 102 is provided with the sleeve 200. The built-in float adapter 102 can be replaced on the outer float frame 101. In use, the built-in float adapter 102 with the required number of sleeves 200 can be replaced on the outer float frame 101 to meet different batch recovery requirements.
[0033] Furthermore, the center of the float body 100 extends toward the exposed side b to form a handle 103. By adding the handle 103, it is convenient to pick up and put down the whole device. The handle 103 is detachably connected to the exposed side b of the outer float frame 101. Furthermore, the handle 103 is a columnar handle 103. The radial outer side of the handle 103 is arc-shaped. In the floating direction, the radial dimension of the handle 103 gradually decreases away from the float body 100 so that the radial outer side of the handle 103 can fit the hand for easy gripping. Furthermore, anti-slip particles 1031 are provided on the handle 103 to ensure the stability of the hand during the recovery process.
[0034] In one embodiment of this utility model, the external float frame 101 adopts a cylindrical structure with the same axial direction and floating direction. The built-in float adapter 102 is a disc-shaped built-in float adapter 102. The inner side of the water bath side a of the external float frame 101 and the outer side of the built-in float adapter 102 are threadedly connected. The handle 103 includes a connecting part 1032 and a handle part 1033. The connecting part 1032 is a hollow columnar connecting part 1032. The water bath side a of the connecting part 1032 is provided with an opening 10321. The inner side of the opening 10321 is threadedly connected to the outer side of the external float frame 101. The center of the exposed side b of the connecting part 1032 extends outward along the floating direction to form the handle 103. The connecting part 1032, the built-in float adapter 102, and the external float frame 101 form a floating cavity 100a. The floating cavity 100a is connected to the storage cavity 200a.
[0035] To improve the sealing effect of the floating cavity 100a, the water bath side a of the outer float frame 101 extends radially outward to form an abutment portion 1011. The radial outer side of the abutment portion 1011 is flush with the radial outer side of the connecting portion 1032, and the exposed side b of the abutment portion 1011 and the water bath side a of the connecting portion 1032 are sealed and matched.
[0036] To improve the stability of the device floating in water, the sleeves 200 are further distributed at equal intervals on the water bath side a of the float body 100. In one embodiment of this utility model, see... Figure 4 The built-in float adapter 102 is a disc-shaped built-in float adapter 102, and the sleeves 200 are arranged at equal intervals along the circumference of the built-in float adapter 102, wherein one of the sleeves 200 corresponds to the center of the built-in float adapter 102.
[0037] Furthermore, the water-resistant float is equipped with a timing module, which is configured to start timing when the water-resistant float comes into contact with the liquid medium. During use, the timing module starts timing simultaneously with the water-bath heating of the water-resistant float in contact with the liquid medium, so that the operator can accurately judge the water-bath heating time of the cell cryopreservation container. Furthermore, the timing module is located inside the handle 103, integrating the timing module into the handle 103 for easy operation and to reduce the overall space occupied by the device. An adjustment button 1034 is provided on the handle 103, which controls the connection to the timing module.
[0038] In one embodiment of this utility model, the adjustment button 1034 includes a start / stop button, a minute setting button, and a second setting button for measuring and controlling time. The hand handle 103 is provided with a display screen 1035 electrically connected to the timing module for displaying time.
[0039] In summary, compared with traditional water bath resuscitation, this water-isolated float for cell resuscitation can perform water-isolated resuscitation without affecting the heat conduction effect, thus solving the problem of introducing contaminants into the water bath during cell resuscitation. Compared with traditional waterless resuscitation, the water-isolated float for cell resuscitation can still achieve the same resuscitation effect for relatively sensitive and fragile cells as direct water bath resuscitation, and the cost is lower than that of waterless resuscitation devices.
[0040] The water bath resuscitation method using a water-resistant float achieves resuscitation effects comparable to direct water bath resuscitation for relatively sensitive and fragile cells without affecting heat conduction.
[0041] The embodiments described above are only used to illustrate the technical ideas and features of this utility model. Their purpose is to enable those skilled in the art to understand the content of this utility model and implement it accordingly. The scope of patent application of this utility model should not be limited by these embodiments. That is, any equivalent changes or modifications made in accordance with the spirit disclosed in this utility model still fall within the patent scope of this utility model.
Claims
1. A water bath resuscitation float for cells, characterized by, It includes a float body (100), on which a floating direction is provided, and the two sides of the floating direction are a water bath side (a) and an exposed side (b). The float body (100) is provided with a sleeve (200) extending toward the water bath side (a), and the sleeve (200) is provided with a storage cavity (200a) that matches the cell cryopreservation container. The float body (100) and the sleeve (200) are sealed and detachably connected to open and close the storage cavity (200a) of the sleeve (200), wherein the number of sleeves (200) is adjustable.
2. A water barrier float for cell water bath recovery according to claim 1, wherein The float body (100) includes an external float frame (101) and a built-in float adapter (102). The built-in float adapter (102) is provided with the sleeve (200). The built-in float adapter (102) can be replacedly installed on the external float frame (101).
3. A water barrier float for cell water bath recovery according to claim 2, wherein The center of the float body (100) extends toward the exposed side (b) to form a handle (103), and the handle (103) and the exposed side (b) of the outer float frame (101) are detachably connected.
4. A water barrier float for cell water bath recovery according to claim 3, wherein The handle (103) is columnar, and the radial outer side of the handle (103) is arc-shaped, wherein in the floating direction, the radial dimension of the handle (103) gradually decreases away from the float body (100).
5. A water barrier float for cell water bath recovery according to claim 4, wherein The hand handle (103) is provided with anti-slip particles (1031).
6. A water barrier float for cell water bath recovery according to claim 3, wherein The external float frame (101) adopts a cylindrical structure with the same axial direction and floating direction. The built-in float adapter (102) is disc-shaped. The inner side of the water bath side (a) of the external float frame (101) and the outer side of the built-in float adapter (102) are threaded together. The hand handle (103) includes a connecting part (1032) and a handle part (1033). The connecting part (1032) is hollow columnar, and the water bath side (a) of the connecting part (1032) is provided with an opening. (10321), the inner side of the opening (10321) and the outer side of the external float frame (101) are threaded together. The center of the exposed side (b) of the connecting part (1032) extends outward along the floating direction to form the hand handle (103). The connecting part (1032), the built-in float adapter (102), and the external float frame (101) form a floating cavity (100a). The floating cavity (100a) and the storage cavity (200a) are connected.
7. A water-resistant float for cell resuscitation in a water bath according to claim 6, characterized in that, The water bath side (a) of the external float frame (101) extends radially outward to form an abutment (1011), the radial outer side of the abutment (1011) is flush with the radial outer side of the connecting part (1032), and the exposed side (b) of the abutment (1011) and the water bath side (a) of the connecting part (1032) are sealed and matched.
8. A water barrier float for cell water bath recovery according to claim 1, wherein The sleeves (200) are evenly distributed on the water bath side (a) of the float body (100).
9. A water barrier float for cell water bath recovery according to claim 3, wherein The water-resistant float is provided with a timing module, which is configured to start timing when the water-resistant float comes into contact with the liquid medium, and the timing module is located inside the hand handle (103).
10. A water barrier float for cell water bath recovery according to claim 9, wherein The hand handle (103) is provided with adjustment buttons (1034), which include a start / stop button, a minute setting button and a second setting button. The hand handle (103) is provided with a display screen (1035) that is electrically connected to the timing module.