Enzyme activity detection kit

Abstract

The utility model provides an enzyme activity detection kit and relates to the technical field of enzyme activity detection. Comprising a box body and a box cover, one side of the box body is an open end, a baffle is hinged to the box body and used for blocking the open end, a mounting plate is fixedly arranged in the box body, a plurality of strip-shaped grooves are formed in the mounting plate, the ends, close to the baffle, of the strip-shaped grooves are open ends, and fixing assemblies are arranged in the strip-shaped grooves in a sliding mode; the fixing assembly comprises clamping pieces and a bearing plate which are symmetrically arranged, a space used for containing an enzyme detection test tube is formed between the two hinged pieces, the bottoms of the clamping pieces are hinged to the top of the bearing plate, the fixing assembly is in sliding fit with the inner wall of the strip-shaped groove, and a first silica gel pad is arranged at the end, away from the clamping pieces, in the strip-shaped groove; the clamping piece abuts against the first silica gel pad. The enzyme detection test tube box is simple in structure, facilitates taking and placing of enzyme detection test tubes, and can guarantee that the enzyme detection test tubes are stably fixed.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, and in particular to an enzyme activity detection kit. Background Technology

[0002] Enzyme activity assays are important techniques in biochemical and molecular biology research, widely used in drug screening, disease diagnosis, and enzyme characterization. Among these, the assay of antiviral enzymes (such as RNase H, proteinase K, and reverse transcriptase) is of great significance for antiviral drug development, research on viral replication mechanisms, and clinical testing.

[0003] Currently, most commercially available enzyme activity assay kits use plastic boxes or foam pads to store enzyme tubes and other reagents. Existing kits use compartmentalized plastic boxes to store enzyme tubes and reaction buffers, with simple grooves fixing the tube positions. However, this traditional design has the following technical drawbacks in practical use:

[0004] Enzyme tubes (especially 1.5mL or 2mL tubes) are typically long and thin, and in standard reagent kits, they are secured only by simple grooves or foam padding, lacking a stable clamping structure. When handling these tubes, researchers often need to use tweezers, or even both hands, significantly impacting detection efficiency. During kit transport or movement, conventional securing methods cannot effectively cushion vibrations, causing enzyme tubes to tilt, shift, or even break. This is particularly true for lyophilized enzyme preparations, where vibration can cause powder to detach, affecting the accuracy of subsequent assays. Utility Model Content

[0005] This invention provides an enzyme activity detection kit that enables rapid placement and removal of enzyme test tubes while ensuring stable fixation.

[0006] An enzyme activity detection kit includes a box body and a lid. One side of the box body is open, and a baffle is hinged to the box body to seal the open end. A mounting plate is fixed inside the box body, and multiple strip grooves are formed on the mounting plate. The end of each strip groove near the baffle is an open end. A fixing component is provided in the strip groove via a sliding assembly. The fixing component includes a support plate and two clamping members. The clamping members are symmetrically arranged along the central axis of the support plate, and a space for placing enzyme detection test tubes is formed between the two clamping members. The bottom of the clamping member is hinged to the top of the support plate. The fixing component slides against the inner wall of the strip groove. A first silicone pad is provided at the end of the strip groove away from the clamping member, and the clamping member abuts against the first silicone pad.

[0007] This invention features a mounting plate with grooves for mounting enzyme test tubes. Fixing components are slidably installed within these grooves, ensuring stable clamping of the test tubes while facilitating storage and retrieval. The fixing components are hinged, allowing them to open to the sides when separated from the grooves for easy placement and removal of the test tubes; when positioned between the grooves, they close together to secure the test tubes, ensuring stable placement. The fixing components hold the upper part of the test tube, while the lower part is secured by a first silicone pad. The radial dimension of the first silicone pad is adapted to the diameter of the test tube. This invention has a simple structure, facilitating both easy placement and removal of the test tubes and ensuring stable fixation.

[0008] Furthermore, a limiting slide is provided at the bottom of the support plate, the length of the limiting slide is greater than the axial length of the support plate, and the limiting slide is slidably connected to the groove wall of the strip.

[0009] To facilitate the sliding of the support plate along the bottom of the strip groove and prevent deviation during the sliding process, a limiting slide bar is installed at the bottom of the support plate.

[0010] Furthermore, at least one limiting slide bar is provided, and the limiting slide bar is arranged axially along the bottom of the support plate along the strip groove. The bottom of the strip groove is provided with a sliding groove, and the limiting slide bar slides in cooperation with the sliding groove.

[0011] One, two, or more limit sliders can be set at the bottom of the support plate. When there are two or more limit sliders, they should be set symmetrically.

[0012] Furthermore, a second silicone pad is provided inside the clamping member, and the second silicone pad is in contact with the inner wall of the clamping member.

[0013] The second silicone pad is used to enhance the elasticity within the clamping component, ensuring that the clamped enzyme test tube can be placed stably and stably during transportation.

[0014] Furthermore, the first silicone pad abuts against the second silicone pad, and the inner surfaces of the first silicone pad and the second silicone pad are on the same horizontal plane.

[0015] The inner surfaces of the first and second silicone pads are on the same horizontal plane, which ensures that the enzyme test tube is on the same horizontal plane when it is laid horizontally, and that there will be no vibration displacement along the radial direction of the tube.

[0016] When the clamps are put together, they form a groove with a semi-circular cross-section. The end of the groove near the opening of the strip groove is closed, and the end near the first silicone pad is open, forming a plane with the first silicone pad. The enzyme test tube can be placed horizontally on it stably. The size of the groove is adapted to the size of the enzyme test tube.

[0017] Furthermore, the inner wall of the clamping member is arc-shaped, and the side of the clamping member away from the support plate is provided with protruding teeth, and the side of the protruding teeth near the enzyme test tube is arc-shaped.

[0018] The serrated edges are used to radially restrict the test tube, creating a ring-like constraint around it and ensuring stable placement of the enzyme test tube. The curved surface at the bottom of the serrations is used to conform to the test tube wall, forming a stable fit.

[0019] Furthermore, the clamping member is provided with a first fixing area and a second fixing area. The diameter of the first fixing area is larger than the diameter of the second fixing area. The size of the first fixing area is adapted to the cap of the enzyme test tube, and the size of the second fixing area is adapted to the body of the enzyme test tube.

[0020] Furthermore, the first silicone pad is in contact with the inner wall of the groove.

[0021] The first silicone pad is glued inside the strip groove, forming a buffer between the enzyme test tube and the strip groove, ensuring the stability of the enzyme test tube during transportation and reducing vibration and bumps.

[0022] Furthermore, rotating parts are provided on both sides of the baffle, and fixing parts are provided at corresponding positions on the box body, with the rotating parts and fixing parts engaging with each other.

[0023] Furthermore, the rotating component has a bent portion at its end, and a shaft is connected to the base of the rotating component. The shaft is rotatably connected to the baffle via a bearing.

[0024] The rotating part has a bend at its end. When the baffle blocks the open end of the box, the bend of the rotating part hooks into the annular groove of the fixing part to form a detachable connection.

[0025] Beneficial effects of utility model

[0026] This invention features a mounting plate with slotted grooves within which fixing components are slidably installed. The fixing components are hinged, allowing the two clamping parts to open to the sides when separated from the grooves, facilitating the placement and removal of the enzyme test tube. When positioned between the grooves, they close together, securing the enzyme test tube and ensuring stable placement. This invention has a simple structure, facilitating both the placement and removal of the enzyme test tube and ensuring its stable fixation. Attached Figure Description

[0027] Figure 1 This is a plan view of the box body of this utility model;

[0028] Figure 2 This is a cross-sectional schematic diagram of the present invention;

[0029] Figure 3 This is an enlarged view of point A in this utility model;

[0030] Figure 4 This is a schematic diagram of the structure of the support plate of this utility model;

[0031] Reference numerals: 1-box body, 2-mounting plate, 3-first silicone pad, 4-clamping component, 5-second silicone pad, 6-baffle, 7-protruding tooth, 8-first fixing area, 9-second fixing area, 10-fixing component, 11-rotating component, 12-strip groove, 13-bearing plate, 14-limiting slide bar, 15-annular groove. Detailed Implementation

[0032] Example 1

[0033] like Figure 1 As shown, an enzyme activity detection kit includes a box body 1 and a lid. One side of the box body 1 is open, and a baffle 6 is hinged to the box body 1 to seal the open end. A mounting plate 2 is fixed inside the box body 1, and multiple strip-shaped grooves 12 are formed on the mounting plate 2. The end of each strip-shaped groove 12 near the baffle 6 is open, and a fixing component is provided inside the strip-shaped groove 12 via a sliding component.

[0034] The fixing assembly includes a support plate 13 and two clamping members 4. The clamping members 4 are symmetrically arranged along the central axis of the support plate 13, and a space for placing enzyme test tubes is formed between the two clamping members 4. The bottom of the clamping member 4 is hinged to the top of the support plate 13. The fixing assembly is slidably engaged with the inner wall of the strip groove 12. A first silicone pad 3 is provided at one end of the strip groove 12 away from the clamping member 4, and the clamping member 4 abuts against the first silicone pad 3.

[0035] An installation plate 2 is installed inside the box body 1. Seven strip grooves 12 are formed on the installation plate 2. One end of each strip groove 12 is open, and the end of the box body 1 closest to the open end of the strip groove 12 is an open end, which is also provided with a baffle 6 for sealing the open end. A fixing component for fixing enzyme test tubes is slidably installed inside the strip groove 12. The fixing component includes two symmetrically arranged clamping parts 4 and a support plate 13. The bottoms of the two clamping parts 4 are respectively hinged to the support plate 13. The fixing component slides in cooperation with the inner wall of the strip groove 12. The size of the fixing component is adapted to the strip groove 12. A first silicone pad 3 is also installed inside the strip groove 12. The first silicone pad 3 abuts against the two clamping parts 4. The radial dimension of the first silicone pad 3 is adapted to the diameter of the enzyme test tube.

[0036] When storing enzyme test tubes, the fixing component is slid out from the open end of the strip groove 12. The clamping member 4, hinged to the support plate 13, opens outwards at the hinge point due to the removal of the restriction of the strip groove 12, expanding the space between the two clamping members 4 to facilitate the placement of the enzyme test tube. After the enzyme test tube is placed, the clamping members 4 on both sides are closed by hand, reducing the space between them and just securing the head and top of the enzyme test tube. The fixing component is then pushed into the strip groove 12, and the end of the enzyme test tube moves along the surface of the first silicone pad 3 towards the closed end of the strip groove 12. Finally, the fixing component is restricted by the side walls of the strip groove 12, securing the head and top of the enzyme test tube, with the end of the enzyme test tube located within the first silicone pad 3. Then, the baffle 6 is pulled up and fixed to the box body 1, and the mounting plate 2 is fixed inside the box body 1.

[0037] When extracting the enzyme test tube, the fixing component is slid out from the open end of the strip groove 12. The clamping member 4, which is hinged to the support plate 13, will open outward at the hinge point because it is no longer restricted by the strip groove 12. The space between the two clamping members 4 becomes larger, and the enzyme test tube is exposed, which facilitates the extraction of the enzyme test tube.

[0038] Example 2

[0039] Based on Example 1, such as Figure 2 As shown, a limiting slide bar 14 is provided at the bottom of the support plate 13. The length of the limiting slide bar 14 is greater than the axial length of the support plate 13, and the limiting slide bar 14 is slidably connected to the groove wall of the strip groove 12. At least one limiting slide bar 14 is provided, and the limiting slide bar 14 is axially arranged at the bottom of the support plate 13 along the strip groove 12. A sliding groove is provided at the bottom of the strip groove 12, and the limiting slide bar 14 slides in cooperation with the sliding groove.

[0040] A limiting slide bar 14 is fixed at the bottom of the support plate. The axis of the limiting slide bar 14 is parallel to the axis of the strip groove 12. A sliding groove is opened in the strip groove 12 at the position directly opposite the limiting slide bar 14. The length of the sliding groove is the same as the length of the strip groove 12. The length of the limiting slide bar 14 is the same as the length of the sliding groove. The limiting slide bar 14 and the sliding groove slide together. The cross-sections of the limiting slide bar 14 and the sliding groove are both trapezoidal.

[0041] Alternatively, three limiting slide bars 14 can be set at the bottom of the support plate 13. One limiting slide bar 14 is fixed at the lowest point of the bottom of the support plate 13, and two limiting slide bars 14 are symmetrically fixed on both sides of the bottom of the support plate 13. On the inner wall of the strip groove 12, sliding grooves are respectively opened at positions directly opposite to the three limiting slide bars 14 to form three sets of sliding fits, thereby ensuring that the support plate 13 can slide stably in the strip groove 12 and preventing displacement during the sliding process.

[0042] Example 3

[0043] Based on Example 1, such as Figure 1 As shown, the inner wall of the clamping member 4 is arc-shaped, and a protruding tooth 7 is provided on the side of the clamping member 4 away from the support plate 13. The side of the protruding tooth 7 near the enzyme test tube is arc-shaped. The clamping member 4 is provided with a first fixing area 8 and a second fixing area 9. The diameter of the first fixing area 8 is larger than the diameter of the second fixing area 9. The size of the first fixing area 8 is adapted to the test tube cap, and the size of the second fixing area 9 is adapted to the test tube body.

[0044] The inside of the clamp 4 is an arc surface, which fits against the outer wall of the enzyme test tube. A first fixing area 8 and a second fixing area 9 are provided on the arc surface of the clamp 4, used to fit and fix the cap and body of the enzyme test tube, respectively. This ensures that the clamp 4 fits tightly against all parts of the enzyme test tube.

[0045] Example 4

[0046] Based on Example 1, such as Figure 1 and Figure 3 As shown, rotating parts 11 are provided on both sides of the baffle 6, and fixing parts 10 are provided at corresponding positions on the box body 1. The rotating parts 11 are engaged with the fixing parts 10. The rotating parts 11 have a bent portion at their ends, and the base of the rotating parts 11 is connected to a shaft. The shaft is rotatably connected to the baffle 6 through a bearing.

[0047] The baffle 6 is hinged to the bottom edge of the box body 1 at its middle section, allowing the baffle 6 to rotate and expose its open end, facilitating the entry and exit of the fixing component into the strip groove 12, thereby enabling the placement and removal of enzyme test tubes. Rotating components 11 with bent ends are installed on both sides of the baffle 6. The base of the rotating component 11 is welded to the shaft core, and the shaft core and baffle 6 are connected by bearings, thus forming a rotatable connection between the rotating component 11 and the baffle 6. Fixing components 10 are installed at corresponding positions on the box body 1. The fixing component 10 is cylindrical, and an annular groove 15 is radially formed in the middle of the fixing component 10. The bent part of the rotating component 11 is embedded into the annular groove 15 on the fixing component 10, forming a snap-fit ​​connection.

Claims

1. An enzyme activity test kit comprising a box body (1) and a box cover, characterized in that, One side of the box body (1) is open. A baffle (6) is hinged to the box body (1) to seal the open end. An installation plate (2) is fixed inside the box body (1). The installation plate (2) has multiple slots (12). The end of the slot (12) near the baffle (6) is open. A fixing component is slidably disposed inside the slot (12). The fixing component includes a support plate (13) and a clamping member (4). There are two clamping members (4). The clamping members (4) are symmetrically arranged along the central axis of the support plate (13). A space for placing enzyme test tubes is formed between the two clamping members (4). The bottom of the clamping member (4) is hinged to the top of the support plate (13). The fixing component is slidably engaged with the inner wall of the strip groove (12). A first silicone pad (3) is provided at one end of the strip groove (12) away from the clamping member (4). The clamping member (4) abuts against the first silicone pad (3).

2. The enzymatic activity assay kit according to claim 1, characterized in that, The bottom of the support plate (13) is provided with a limiting slide (14), the length of the limiting slide (14) is greater than the axial length of the support plate (13), and the limiting slide (14) is slidably connected to the groove wall of the strip groove (12).

3. The enzymatic activity assay kit according to claim 2, characterized in that, At least one limiting slide bar (14) is provided. The limiting slide bar (14) is axially arranged at the bottom of the bearing plate (13) along the strip groove (12). A sliding groove is provided at the bottom of the strip groove (12). The limiting slide bar (14) slides in cooperation with the sliding groove.

4. The enzymatic activity assay kit of claim 1, wherein, A second silicone pad (5) is provided inside the clamping member (4), and the second silicone pad (5) is in contact with the inner wall of the clamping member (4).

5. The enzymatic activity assay kit according to claim 4, characterized in that, The first silicone pad (3) abuts against the second silicone pad (5), and the inner surfaces of the first silicone pad (3) and the second silicone pad (5) are on the same horizontal plane.

6. The enzymatic activity assay kit of claim 1, wherein, The inner wall of the clamping member (4) is arc-shaped, and the end of the clamping member (4) away from the bearing plate (13) is provided with a protruding tooth (7). The side of the protruding tooth (7) close to the enzyme test tube is arc-shaped.

7. The enzyme activity detection kit according to claim 6, characterized in that, The clamp (4) is provided with a first fixing area (8) and a second fixing area (9). The diameter of the first fixing area (8) is larger than the diameter of the second fixing area (9). The size of the first fixing area (8) is adapted to the cap of the enzyme test tube, and the size of the second fixing area (9) is adapted to the body of the enzyme test tube.

8. The enzymatic activity assay kit of claim 1, wherein, The first silicone pad (3) is in contact with the inner wall of the groove (12).

9. The enzymatic activity assay kit of claim 1, wherein, Rotating components (11) are provided on both sides of the baffle (6), and fixing components (10) are provided at corresponding positions on the box body (1). The rotating components (11) are engaged with the fixing components (10).

10. The enzymatic activity assay kit according to claim 9, characterized in that, The rotating part (11) has a bent part at its end, and the base of the rotating part (11) is connected to a shaft core. The shaft core is rotatably connected to the baffle (6) through a bearing.

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