Detection device capable of releasing double reagents in stages

Abstract

A detection device capable of releasing double reagents in stages comprises a reaction tube and a through knife, the reaction tube comprises an upper tube, a transition connecting tube and a lower tube, a second reagent and a first reagent are arranged in the upper tube and the transition connecting tube respectively, the lower tube is a filtering reaction tube, a sealing film is arranged at the lower end of the transition connecting tube to isolate the first reagent from the lower tube, and the through knife is a sealing rod through knife. The lower end of the sealing rod through knife is a puncturing part, the middle part of the sealing rod through knife is provided with an annular sealing part capable of performing stage sealing on a second reagent, and the annular sealing part is always sealed with the lower end of the upper tube before the sealing rod through knife punctures the sealing membrane and when the first reagent flows into the lower tube; the sealing between the annular sealing part and the upper tube is released, the second reagent flows into the lower tube, and the upper part of the sealing rod through knife is provided with a staged stop bulge. The device is reasonable in structure, convenient to operate and high in detection precision, and realizes physical isolation of double reagents, staged release and efficient treatment of small samples by improving the through knife structure of the sealing rod and adding a waste liquid separation structure.

Description

Technical Field

[0001] This utility model belongs to the fields of bioreactors and chemical engineering technology, and relates to a detection device that releases dual reagents in stages. It is suitable for microbial samples, cell nucleic acid extraction, enzyme-linked immunosorbent assay (ELISA) and biological sample filtration, small sample centrifugation and other chemical processes that require the release of dual reagents in stages. Background Technology

[0002] In biological detection (such as microbial samples, cell nucleic acid extraction, enzyme-linked immunosorbent assays) and chemical production (chemical synthesis reactions; chemical product quality testing; fine chemical production), reagents often need to be added in a specific order to avoid interference from pre-reactions between components. For example, in quantitative fluorescence detection, if the fluorescent dye comes into contact with the lysis buffer prematurely, it may lead to fluorescence quenching or a decrease in reaction efficiency. Another example is chemical synthesis reactions: in drug synthesis, some reactions require the addition of a catalyst first, followed by the addition of another reactant after the reaction has progressed to a certain extent. This device allows for precise control of the addition time and dosage of the two reagents, improving the selectivity and yield of the reaction. In the production of fine chemicals, such as cosmetics and fragrances, precise control of the order and amount of reagent addition is often necessary. For example, in fragrance synthesis, a precursor substance with a specific fragrance is added first, followed by another reagent to react and generate the final fragrance product.

[0003] Meanwhile, in response to the serious problem of high false negative rates in ATP testing caused by the interference of high ion residues of disinfectant on ATP luciferase during the application process, a further improved solution has been developed.

[0004] The applicant's existing Chinese patent, CN201711318648.3, entitled "A Separation and Preservation Reagent Filtration and Detection Device," includes a sealing cap, a filter pump head, and a filter reaction tube. The sealing cap is compressible and contains a first-class reagent. The filter reaction tube is located inside the filter pump head, with a sealing plug at the bottom. The filter reaction tube contains a filter membrane capable of trapping microorganisms. The upper end of the filter reaction tube is connected to the sealing cap via a support ring. The bottom of the support ring has a sealing isolation layer for placing a second-class reagent. A hollow sealing rod is located inside the support ring and the sealing cap. The upper end of the sealing rod has a top cover for the sealing layer, with a sharp protrusion on the top cover for piercing the sealing layer. The inner wall of the top of the sealing cap has an inverted triangular protrusion, and the bottom of the sealing rod has a protrusion for piercing the isolation layer.

[0005] Meanwhile, the Chinese patent application of the applicant, entitled "A Rotary Separation and Preservation Reagent Detection Device" (patent number CN202222654729.3), includes a circular tube, a connecting tube, a reaction tube, and a plug. Its features include: a rotating cylinder connected to the upper part of the circular tube, and the circular tube containing a first-class reagent; a connecting groove provided at the top of the rotating cylinder, and a through-blade installed within the connecting groove; the upper part of the through-blade is a connecting portion that can cooperate with the connecting groove, and the bottom is provided with a through-blade thread, with a sharp protrusion below the through-blade thread; the bottom of the circular tube is provided with a connecting hole with internal threads that connects to the through-blade.

[0006] Both of the aforementioned patents involve directly mixing the two reagents by piercing the sealing layer, and then reacting the mixed reagents with the analyte. The original invention is suitable for applications where the transition reaction tube contains lyophilized powder and the upper tube contains sterile water, but it is not suitable for applications involving two stages of reagent release. Therefore, the above structure has the following drawbacks during use:

[0007] First, the second reagent and the first reagent may come into slight contact before release due to seal failure, leading to a pre-reaction.

[0008] Second, the timing of reagent release depends on a single puncture action, making it impossible to control the reaction process in stages, which affects the detection accuracy.

[0009] Third, the lack of a waste liquid separation structure during sample processing makes it inconvenient for centrifugation of small sample volumes.

[0010] Fourth, this system can only satisfy the requirement of reacting the two reagents together with the analyte, and cannot satisfy the requirement of the two reagents being released in stages and reacting with the analyte. Therefore, based on the above two patents, further improvements have been made, making it applicable to a wider range of fields. Utility Model Content

[0011] The technical problem to be solved by this utility model is to provide a detection device that releases dual reagents in stages, which has a reasonable structure, is easy to operate, and has high detection accuracy, for adding reagents in stages in biochemical reactions or other chemical reactions.

[0012] The technical solution adopted by this utility model to solve the above-mentioned technical problems is as follows: a detection device for phased release of dual reagents, comprising a reaction tube and a through-blade, characterized in that: the reaction tube includes an upper tube, a transition connecting tube, and a lower filter reaction tube with a waste liquid separation structure; the upper tube and the transition connecting tube respectively contain a second reagent and a first reagent; the lower tube is a filter reaction tube with a filter reaction chamber inside; the lower end of the transition connecting tube is provided with a sealing membrane to isolate the first reagent from the filter reaction chamber; the through-blade is a sealing rod through-blade, and the lower end of the sealing rod through-blade is a piercing... The upper tube has an annular sealing section in the middle section to seal the second reagent inside. Before the sealing rod pierces the sealing membrane, and during the flow of the first reagent into the lower tube of the filtration reaction, the annular sealing section of the sealing rod pierces the sealing membrane. When the sealing rod continues to move down to the position where the top of the sealing rod is level with the sealing cap of the upper tube, the seal between the annular sealing section and the middle sealing ring of the upper tube is released, and the second reagent flows smoothly into the lower tube of the filtration reaction through the transition connecting tube. A staged stop protrusion is provided on the upper part of the sealing rod piercing section.

[0013] As an improvement, the upper part of the sealing rod through-blade is a polygonal prism, the diameter of the annular sealing part is larger than the outer diameter of the upper part of the sealing rod through-blade, the upper end of the upper tube is open, and the lower end of the upper tube is provided with a hollow groove for the second reagent to fall. The lower end of the upper tube, corresponding to the hollow groove, is formed with a circular sleeve that mates with the annular sealing part of the sealing rod through-blade. The annular sealing part adopts an external thread structure, and the inner wall of the circular sleeve is formed with a corresponding internal sealing thread. The sealing rod through-blade seals with the lower end of the upper tube through the thread structure.

[0014] Furthermore, the lower end of the sealing rod through-knife is a triangular prism or a blade-shaped piercing part with a tip angle of 30~60°. The annular sealing part is located above and close to the piercing part. A rotatable top cover is provided at the upper end opening of the upper tube. A connecting post with a prismatic hole protrudes from the bottom surface of the top cover. The upper end of the sealing rod through-knife is inserted into the prismatic hole, and the sealing rod through-knife is rotated by the rotation of the top cover.

[0015] Furthermore, the inner wall of the upper opening of the upper tube is provided with an annular groove, and the outer wall of the lower end of the top cover is provided with a corresponding rib. The top cover is rotatably connected to the upper tube through the rib and the groove.

[0016] Furthermore, the staged stop protrusions are two in number, one above the other. When the sealing rod cutter moves down to the first stop protrusion, the sealing membrane is punctured, and the first reagent flows into the lower tube to react. When the sealing rod cutter continues to move down to the second stop protrusion, the seal between the annular sealing part and the upper tube is released, and the second reagent flows into the lower tube to react.

[0017] Furthermore, the sample to be reacted in the lower tube can be sampled by filtration or by smearing. The lower end of the lower tube is provided with a filter membrane or smearing head with the sample, and the tail end is provided with a silicone plug.

[0018] Furthermore, the filtration sampling process is as follows: the upper opening of the filtration reaction tube is connected to the container bottle through the container bottle nozzle, and a filter membrane is set at the lower opening of the tube. After filtration, the sample remains on the filter membrane. The smear head for smear sampling is equipped with a filter membrane and a flocked sampling layer. A detachable smearing rod is inserted into the hollow cavity of the smear head. After smear sampling is completed, the smear head is inserted into the lower opening of the tube, the smearing rod is removed, and the outer wall of the smear head and the inner wall of the lower opening of the tube are positioned by a groove.

[0019] Furthermore, the waste liquid separation structure includes a waste liquid tube, and the lower end of the filter reaction tube is provided with a snap-fit ​​interface on the outer wall. The snap-fit ​​interface has a concave-convex locking structure. The inner wall of the waste liquid tube is a snap-fit ​​centrifuge tube that is connected to the snap-fit ​​interface. It is suitable for processing samples ≤2ml and can withstand centrifugation force ≥10000 rpm.

[0020] Furthermore, the bottom of the lower tube is provided with a negative pressure interface for connection to a negative pressure pump, which is adapted to external pump body to process ≥2ml samples, and the auxiliary target object is rinsed with sterile physiological saline and switched to centrifugation mode.

[0021] Finally, the annular sealing part is not limited to a threaded structure, but can also adopt a nested silicone rubber sealing ring structure. The middle part of the sealing rod through-hole forms an interference sliding seal with the through hole at the lower end of the upper tube through the silicone rubber sealing ring. In this case, the sealing rod through-hole is driven by pressing.

[0022] Compared with existing technologies, the advantages of this invention are as follows: The sealing rod through-cutter structure is improved by adding an annular sealing part in the middle that seals with the through-hole at the lower end of the upper tube. This ensures that when the sealing rod through-cutter pierces the transition connection sealing membrane and the first reagent flows into the lower tube, the second reagent remains sealed. A stop protrusion is provided at the rear of the through-cutter. When the sealing rod through-cutter moves down to the first stop protrusion, the sealing membrane is pierced, and the first reagent flows into the lower tube for reaction. When the top of the sealing rod through-cutter moves down to the second stop protrusion and is level with the top cover of the upper tube, the seal between the annular sealing part and the upper tube is released, and the second reagent flows into the lower tube for reaction. A waste liquid separation structure is added to the lower tube to facilitate centrifugation of small samples. This invention has a reasonable structure, is easy to operate, and has high detection accuracy. By improving the sealing rod through-cutter structure and adding a waste liquid separation structure, it achieves physical isolation of the two reagents, staged release, and efficient processing of small samples. Attached Figure Description

[0023] Figure 1 This is a structural schematic diagram of an embodiment of the present utility model;

[0024] Figure 2 This is a structural cross-sectional view of an embodiment of the present utility model;

[0025] Figure 3This is a cross-sectional view of the present invention in the state of piercing the sealing film and releasing the first reagent;

[0026] Figure 4 This is a cross-sectional view of the present invention in the state of releasing the second reagent;

[0027] Figure 5 This is a schematic diagram of the upper pipe structure;

[0028] Figure 6 yes Figure 5 A sectional view;

[0029] Figure 7 This is a schematic diagram of the transition tube structure;

[0030] Figure 8 yes Figure 7 A sectional view;

[0031] Figure 9 This is a schematic diagram of the structure in the filtered state;

[0032] Figure 10 yes Figure 9 A sectional view;

[0033] Figure 11 This is a structural diagram of the top cover;

[0034] Figure 12 yes Figure 11 A sectional view;

[0035] Figure 13 This is a schematic diagram of the structure under centrifugal conditions;

[0036] Figure 14 yes Figure 13 A sectional view;

[0037] Figure 15 This is a structural diagram of the sealing rod through-hole tool;

[0038] Figure 16 This is a cross-sectional view of the applicator head and applicator stick. Detailed Implementation

[0039] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0040] like Figures 1-16As shown, a detection device for phased release of dual reagents can perform biochemical reactions or other chemical reactions by adding reagents in stages. It includes a reaction tube A and a through-blade 4. The reaction tube A includes an upper tube 1, a transition connecting tube 2, and a lower tube 3 with a waste liquid separation structure. The upper tube 1 and the transition connecting tube 2 respectively contain a second reagent and a first reagent. The lower tube 3 is a filter reaction tube with a filter reaction chamber. The lower end of the transition connecting tube 2 is equipped with a sealing membrane 22 to isolate the first reagent from the filter reaction chamber. The through-blade 4 is a sealing rod. The sealing rod through-knife 4 has a piercing part 41 at its lower end and an annular sealing part 42 in its middle part to seal the second reagent in the upper tube 1. Before the sealing rod through-knife 4 pierces the sealing film and until the sealing film is pierced and the first reagent flows into the lower tube 3, the annular sealing part 42 of the sealing rod through-knife 4 is always sealed with the lower end of the upper tube 1. When the sealing rod through-knife 4 continues to move down to a certain position, the seal between the annular sealing part 42 and the upper tube 1 is released, and the second reagent flows into the lower tube 3. A staged stop protrusion is provided on the upper part of the sealing rod through-knife 4.

[0041] The specific structure is as follows: The connection structure of the upper pipe 1, the transition connecting pipe 2, and the lower filter reaction pipe 3 is as follows: The transition connecting pipe 2 is divided into upper and lower parts, with the upper diameter larger than the lower diameter. The inner diameter of the upper opening of the lower filter reaction pipe 3 matches the lower part of the transition pipe 2. The inner wall of the upper opening of the lower filter reaction pipe 3 and the lower outer wall of the transition connecting pipe 2 are respectively provided with corresponding grooves. The lower part of the transition connecting pipe 2 is inserted into the lower filter reaction pipe 3 and fixed by the grooves. The lower end of the upper pipe 1 is provided with an extension section 14 that matches the upper part of the transition connecting pipe 3. The outer wall of the extension section 14 and the upper inner wall of the transition pipe 3 are respectively provided with corresponding grooves. The lower end of the upper pipe 1 is inserted into the transition pipe 2 through the extension section 14 and fixed by the grooves. Of course, the groove connection can also be replaced by a threaded connection, with a similar effect.

[0042] The upper part of the sealing rod through-knife 4 is a polygonal prism. The diameter of the annular sealing part 42 is larger than the outer diameter of the upper part of the sealing rod through-knife 4. The upper end of the upper tube 1 is open. A hollow groove 11 is provided in the middle of the lower end face of the upper tube 1 for the second reagent to fall. A circular sleeve 12 is formed at the lower end of the upper tube 1, corresponding to the hollow groove 11, to cooperate with the annular sealing part 42 of the sealing rod through-knife 4. In this embodiment, the annular sealing part 42 adopts an external thread structure. The inner wall of the circular sleeve 12 is formed with a corresponding internal thread. The sealing rod through-knife 4 is sealed to the lower end of the upper tube 1 by a threaded connection, and the sealing rod through-knife 4 can move up and down by rotation. The lower end piercing part 41 of the sealing rod through knife 4 is a triangular prism or blade-shaped piercing part with a tip angle of 30~60°. The annular sealing part 42 is located above and close to the piercing part 41. A rotatable top cover 5 is provided at the upper opening of the upper tube 1. The inner wall of the upper opening of the upper tube 1 is provided with an annular groove 13. The lower outer wall of the top cover 5 is provided with a corresponding groove 51. The top cover 5 is rotatably connected to the upper tube 1 through the groove 13 and the groove 51. The bottom surface of the top cover 5 is provided with a connecting post with a prismatic hole 52. The upper end of the sealing rod through knife 4 is inserted into the prismatic hole 52. The rotation of the top cover 5 drives the sealing rod through knife 4 to rotate.

[0043] There are two stop protrusions in the stage, namely the first stop protrusion 43 and the second stop protrusion 44. When the sealing rod knife 4 moves down to the first stop protrusion, the sealing membrane is punctured, and the first reagent flows into the filter reaction tube 3 to react with the filtered or centrifuged sample. When the top of the sealing rod knife 4 continues to move down to the second stop protrusion and is level with the top cover of the upper tube, the seal between the annular sealing part 42 and the upper tube 4 is released, and the second reagent flows into the lower tube 3 to react. In this embodiment, the second stop protrusion is located at the upper end of the sealing rod knife 4. When the upper end of the sealing rod knife 4 is level with the top cover 5, the sealing rod knife 4 is in the second stop protrusion position. Since the top cover 5 has a reasonable thickness, the sealing rod knife 4 cannot be separated from the top cover. The threaded gap at the lower end of the upper tube 1 is sufficient to allow the second reagent to flow into the lower tube 3 during up-and-down vibration.

[0044] The sample to be reacted in the lower tube 3 of the filtration reaction can be sampled by filtration or by smearing. The lower opening of the lower tube 3 is equipped with a filter membrane or smearing head 8 containing the sample, and the tail end is equipped with a silicone plug. The filtration sampling process is as follows: Figure 11 , 12As shown, the lower tube 3 is connected to the container bottle 6 via the container nozzle 7. A filter membrane is installed at the lower opening of the lower tube 3, and the filtered sample remains on the filter membrane. The smear head 8 for smearing sampling is equipped with a filter membrane 81 and a flocked sampling layer 82. A detachable smearing rod 9 is inserted into the hollow cavity of the smear head. After smearing sampling, the smear head is inserted into the lower opening of the lower tube 3. The outer wall of the smear head and the inner wall of the lower opening of the lower tube 3 are positioned by a groove. After sampling in an environment that has not been sterilized, the smearing rod 9 can be removed directly. After sealing the tail end with a silicone plug, ATP reagent is added for detection. When sampling after sterilization, since the disinfectant affects the ATP luciferase reaction, a corresponding buffer solution needs to be prepared for cleaning. The steps are as follows: after adding the silicone plug, add the buffer solution, remove the plug and place it in a centrifuge tube or waste tube, centrifuge and clean it, and then add ATP reagent for detection.

[0045] The waste liquid separation structure includes a waste liquid tube 10. The lower end of the lower tube 3 is provided with a snap-fit ​​interface 31. The snap-fit ​​interface 31 has a concave-convex locking structure. The waste liquid tube 10 is a snap-fit ​​centrifuge tube that is connected to the snap-fit ​​interface. It is suitable for processing samples ≤2ml and can withstand centrifugation force ≥10000 rpm.

[0046] The bottom of the lower tube 3 is also equipped with a negative pressure interface for connecting to a negative pressure pump, which is compatible with external pumps to process samples ≥2ml and rinses the auxiliary target material with physiological saline before switching to centrifugation mode.

[0047] In addition, the annular sealing part 42 is not limited to a threaded structure, but can also adopt a nested silicone rubber sealing ring structure. The middle part of the sealing rod through knife 4 forms an interference sliding seal with the through hole at the lower end of the upper tube 3 through the silicone rubber sealing ring. At this time, the sealing rod through knife 4 is driven by pressing.

[0048] The working principle is as follows: Rotating the top cover 5 drives the sealing rod and the through knife 4 to rotate and move downwards until the first stop protrusion pierces the sealing membrane, releasing the first reagent, such as... Figure 3 At this point, the external thread of the annular sealing part 42 of the sealing rod knife 4 and the internal thread at the lower end of the upper tube 1 have not completely disengaged, and the second reagent cannot flow out. Continue to rotate the top cover 5, the sealing rod knife 4 rotates and moves down to the second stop protrusion, the sealing rod knife 4 is flush with the top cover 5, the external thread of the annular sealing part 42 of the sealing rod knife 4 and the internal thread at the lower end of the upper tube 1 are completely disengaged, the second reagent is released and flows into the lower tube 3 to react.

[0049] Sample processing:

[0050] Small sample (≤2ML): Inject into the filter reaction chamber of lower tube 3 → connect the snap-on waste liquid tube 10 → centrifuge (3000~10000 rpm, 2 minutes) → remove waste liquid.

[0051] Large samples (≥2 mL): Connect the negative pressure pump for enrichment → rinse with physiological saline → switch to centrifugation mode. After large sample processing is complete, it can be washed and centrifuged again as a small sample.

[0052] Meanwhile, in response to the serious problem of high false negative rates in ATP testing caused by the high ion residue of disinfectant during the smear sampling process, which interferes with ATP luciferase, a solution has been further improved.

[0053] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A detection device for the phased release of two reagents, comprising a reaction tube and a through-blade, characterized in that: The reaction tube includes an upper tube, a transition connecting tube, and a lower filtration reaction tube with a waste liquid separation structure. The upper tube and the transition connecting tube contain a second reagent and a first reagent, respectively. The lower tube is a filtration reaction tube with a filtration reaction chamber inside. The lower end of the transition connecting tube is equipped with a sealing membrane to isolate the first reagent from the filtration reaction chamber. The through-blade is a sealing rod through-blade. The lower end of the sealing rod through-blade is a piercing part, and the middle part is equipped with an annular sealing part that can perform staged sealing of the second reagent in the upper tube. Before the sealing rod through-blade pierces the sealing membrane until the sealing membrane is pierced and the first reagent flows into the lower tube, the annular sealing part of the sealing rod through-blade is always sealed with the lower end of the upper tube. When the sealing rod through-blade continues to move down to a certain position, the seal between the annular sealing part and the upper tube is released, and the second reagent flows into the lower tube. The upper part of the sealing rod through-blade is equipped with a staged stop protrusion.

2. The detection device according to claim 1, characterized in that: The upper part of the sealing rod through-blade is a polygonal prism. The diameter of the annular sealing part is larger than the outer diameter of the upper part of the sealing rod through-blade. The upper end of the upper tube is open, and the lower end of the upper tube has a hollow groove for the second reagent to fall through. The lower end of the upper tube, corresponding to the hollow groove, has a circular sleeve that mates with the annular sealing part of the sealing rod through-blade. The annular sealing part adopts an external thread structure, and the inner wall of the circular sleeve is formed with a corresponding internal thread. The sealing rod through-blade seals with the lower end of the upper tube through the thread structure.

3. The detection device according to claim 2, characterized in that: The lower end of the sealing rod through-knife has a triangular prism or blade-shaped piercing part with a tip angle of 30-60°. The annular sealing part is located above and close to the piercing part. A rotatable top cover is provided at the upper opening of the upper tube. A connecting post with a prismatic hole protrudes from the bottom surface of the top cover. The upper end of the sealing rod through-knife is inserted into the prismatic hole. The rotation of the top cover drives the sealing rod through-knife to rotate.

4. The detection device according to claim 3, characterized in that: The upper opening of the upper tube has an annular groove on its inner wall, and the lower outer wall of the top cover has a corresponding rib. The top cover is rotatably connected to the upper tube through the rib and the groove.

5. The detection device according to claim 4, characterized in that: The staged stop protrusions are two in number, one above the other. When the sealing rod cutter moves down to the first stop protrusion, the sealing membrane is punctured, and the first reagent flows into the lower tube to react. When the sealing rod cutter continues to move down to the second stop protrusion, the seal between the annular sealing part and the upper tube is released, and the second reagent flows into the lower tube to react.

6. The detection device according to claim 1, characterized in that: The sample to be reacted in the lower tube can be sampled by filtration or by smearing. The lower end of the lower tube is equipped with a filter membrane or smearing head with the sample, and the tail end is equipped with a silicone plug.

7. The detection device according to claim 6, characterized in that: The filtration and sampling process is as follows: the lower tube is connected to the container bottle through the container nozzle, and a filter membrane is set at the lower end opening of the lower tube. After filtration, the sample remains on the filter membrane. The smear head for smear sampling is equipped with a filter membrane and a flocked sampling layer. A detachable smearing rod is inserted into the hollow cavity of the smear head. After the smear sampling is completed, the smear head is inserted into the lower end opening of the lower tube, and the smearing rod is removed. The outer wall of the smear head and the inner wall of the lower end opening of the lower tube are positioned by a groove.

8. The detection device according to any one of claims 1 to 7, characterized in that: The waste liquid separation structure includes a waste liquid pipe, and the lower end of the lower pipe is provided with a snap-fit ​​interface. The snap-fit ​​interface has a concave-convex locking structure, and the waste liquid pipe is a snap-fit ​​centrifuge tube that is connected to the snap-fit ​​interface.

9. The detection device according to any one of claims 1 to 7, characterized in that: The bottom of the lower tube is equipped with a negative pressure interface that connects to the negative pressure pump, which is suitable for external pumps to process samples ≥2ml. The auxiliary target material is rinsed with physiological saline or pyrogen-free sterile water and then switched to centrifugation mode.

10. The detection device according to any one of claims 2 to 7, characterized in that: The annular sealing part is not limited to a threaded structure, but can also adopt a nested silicone rubber sealing ring structure. The middle part of the sealing rod through-hole forms an interference sliding seal with the through hole at the lower end of the upper tube through the silicone rubber sealing ring. At this time, the sealing rod through-hole is driven by pressing.

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