Image flow cytometry detection device
By employing techniques such as a spiral delivery structure, a flared tapering design, heating, and a low surface energy coating in the image flow cytometry cell detection device, the problems of adhesion and blockage during sample transport have been solved, achieving efficient and accurate cell detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CHANGDE VOCATIONAL & TECH COLLEGE
- Filing Date
- 2025-04-21
- Publication Date
- 2026-04-24
AI Technical Summary
In existing image flow cytometry cell detection devices, samples are prone to adhesion and blockage during transport, affecting detection efficiency and result accuracy.
It adopts a transparent microchannel system with a spiral conveying structure and a horn-shaped tapering design on the inner wall. Combined with a guide vane and guide shaft with a spiral angle of 45 degrees, it is equipped with a heating rod and a low surface energy coating. It is equipped with an anti-reverse airflow valve and a flow monitoring sensor, uses multi-LED array lighting, and has a waste liquid outlet connected to a self-priming pump. It also has a cleaning interface and a replaceable filter assembly.
It effectively reduces sample adhesion and blockage during transport, ensures unobstructed flow channels, improves detection efficiency and result accuracy, and supports high-quality optical imaging and automated operation.
Smart Images
Figure CN224163542U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of cell detection technology, specifically to an image flow cytometry cell detection device. Background Technology
[0002] Image flow cytometry is a device that uses optical imaging technology combined with fluid dynamics principles to perform high-throughput, high-speed analysis of single cells or other microparticles. However, a technical challenge exists in this process: how to reduce sample adhesion and blockage during transport. Some components in the sample are prone to non-specific adsorption inside the microchannels, or large particles can cause channel blockage, thus affecting detection efficiency and the accuracy of results. Summary of the Invention
[0003] In view of this, embodiments of the present disclosure provide an image flow cytometry cell detection device that at least partially solves the problems existing in the prior art.
[0004] This application discloses an image flow cytometry cell detection device, comprising:
[0005] Sample inlet, used to introduce the sample to be tested;
[0006] A microchannel system for transporting samples; wherein the microchannel system is transparent, has a spiral transport structure on its inner wall, and tapers in a funnel shape near the sample inlet;
[0007] An illumination unit, mounted on the side of the microchannel system, provides uniform illumination to the sample flowing through the microchannel system to facilitate image acquisition;
[0008] The detection unit, located on one side of the downstream section of the microchannel system, is used to capture the optical signal of the sample passing through the microchannel system;
[0009] The waste liquid outlet, located at the end of the microchannel system, is used to discharge the liquid sample that has been tested; among which...
[0010] The spiral conveying structure on the inner wall of the microchannel system includes guide vanes and a guide shaft, wherein a heating rod is installed inside the guide shaft to prevent sample deterioration or sedimentation caused by temperature changes; and
[0011] The spiral conveyor structure also includes multiple micro-protrusion units, which are evenly distributed on the guide vanes.
[0012] Preferably, the spiral conveying structure is a spiral concave structure.
[0013] Preferably, the spiral angle of the guide vane is 45 degrees.
[0014] Preferably, the junction of the flared, tapering inlet and the microchannel system is equipped with an anti-backflow valve.
[0015] Preferably, the microchannel system is coated with a low surface energy coating.
[0016] Preferably, the lighting unit is a multi-LED array.
[0017] Preferably, the waste liquid outlet is connected to a self-priming pump.
[0018] Preferably, the end of the microchannel system is provided with a cleaning port.
[0019] Preferably, a flow monitoring sensor is also installed within the micropipeline system.
[0020] Preferably, a replaceable filter assembly is provided at the connection between the sample inlet and the microchannel system.
[0021] This disclosure provides an image flow cytometry detection device, comprising: a sample inlet for introducing a sample to be tested; a microchannel system for transporting the sample; wherein the microchannel system is transparent, has a spiral transport structure on its inner wall, and tapers in a funnel shape near the sample inlet; an illumination unit installed on the side of the microchannel system to provide uniform illumination to the sample flowing through the microchannel system for image acquisition; a detection unit located on the downstream side of the microchannel system for capturing the optical signal of the sample passing through the microchannel system; and a waste liquid outlet located at the end of the microchannel system for discharging the liquid sample that has been tested; wherein the spiral transport structure on the inner wall of the microchannel system includes a guide plate and a guide shaft, wherein a heating rod is provided in the guide shaft to avoid sample deterioration or sedimentation caused by temperature changes; and the spiral transport structure also includes multiple micro-protrusion units evenly distributed on the guide plate. The solution of this disclosure can solve the problem of reducing sample adhesion and blockage during transport. Attached Figure Description
[0022] In the accompanying drawings, unless otherwise specified, the same reference numerals throughout the various drawings denote the same or similar parts or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings depict only some embodiments disclosed in this application and should not be construed as limiting the scope of this application.
[0023] Figure 1 This is a schematic diagram of the structure of the image flow cytometry cell detection device described in this utility model;
[0024] Figure 2 This is a schematic diagram of the internal structure of the microchannel system in the image flow cytometry cell detection device of this utility model;
[0025] Figure 3 The image flow cytometry cell detection device described in this utility model Figure 2Enlarged view of point A in the middle;
[0026] Figure 4 This is a schematic diagram of the internal structure of the sample inlet in the image flow cytometry detection device of this utility model.
[0027] In the diagram: 1. Sample inlet; 2. Microchannel system; 3. Illumination unit; 4. Detection unit; 5. Waste outlet; 6. Flow guide plate; 7. Anti-reverse flow valve; 8. Low surface energy coating; 9. LED array; 10. Flow guide shaft; 11. Self-priming pump; 12. Cleaning port; 13. Flow monitoring sensor; 14. Micro-protrusion unit; 15. Filter assembly; 16. Heating rod Detailed Implementation
[0028] The embodiments of this disclosure will now be described in detail with reference to the accompanying drawings.
[0029] The following specific examples illustrate the implementation of this disclosure. Those skilled in the art can easily understand other advantages and effects of this disclosure from the content disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of this disclosure, and not all of them. This disclosure can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this disclosure. It should be noted that, in the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.
[0030] It should be noted that various aspects of embodiments within the scope of the appended claims are described below. It will be apparent that the aspects described herein can be embodied in a wide variety of forms, and any particular structure and / or function described herein is merely illustrative. Based on this disclosure, those skilled in the art will understand that one aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number of aspects set forth herein can be used to implement the device and / or practice the method. Additionally, this device and / or method can be implemented using structures and / or functionalities other than one or more of the aspects set forth herein.
[0031] It should also be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of this disclosure. The drawings only show the components related to this disclosure and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.
[0032] Furthermore, specific details are provided in the following description to facilitate a thorough understanding of the examples. However, those skilled in the art will understand that the described aspects can be practiced without these specific details.
[0033] like Figure 1 As shown, the image flow cytometry detection device of this application includes: a sample inlet 1 for introducing a sample to be tested; a microchannel system 2, which includes microchannels for transporting the sample; an illumination unit 3, which is installed on the side of the microchannel system 2 and provides uniform illumination therein for image acquisition; a detection unit 4, located in the downstream portion of the microchannel system 2 to capture optical signals; and a waste liquid outlet 5, located at the end of the microchannel system 2, for discharging the liquid that has been tested.
[0034] The sample inlet 1 connects to the front opening of the microchannel system 2, where the shape gradually tapers in a funnel shape. This tapering design helps to generate vortex flow, suspending particles in the liquid and preventing deposition.
[0035] The microchannel system 2 is constructed from transparent polymer materials with excellent surface smoothness. These materials ensure that the internal structure of the device does not easily trap samples during actual operation and support high-quality imaging under a microscope. The spiral transport texture on the channel wall creates a stirring-like force field in the narrow sections of the flow channel to disperse and drive the sample forward, thereby preventing material sedimentation, accumulation, or blockage.
[0036] The illumination unit 3 is fixedly placed next to the sample path to ensure that the beam can be perpendicularly incident into the space carrying the analyte, thereby illuminating the entire passage point to provide the optimal lighting conditions necessary for subsequent detection.
[0037] The detection unit 4 is located on the same side or opposite side of the light source and is set in the downstream section of the microchannel. It is mainly composed of sensor components and can receive information emitted or scattered back from the object. After processing, it outputs the corresponding electrical signal to represent the quantitative change or qualitative difference information of the observed object's characteristics.
[0038] Waste liquid outlet 5 is set at the end of the microchannel as a termination interface of the kit. After the sample completes the corresponding test process, it leaves the main working space from this position and is discharged from the outside of the system as a waste discharge port, thereby realizing the maintenance of a closed environment in the unidirectional material circulation mode.
[0039] In this application, a pipe design featuring a spiral concave structure and a flared inlet section effectively improves the sample's travel characteristics along the pipe. The introduction of this spiral angle structure not only enhances the dynamic effect of medium transport to a certain extent but also disturbs the turbulent flow near the wall layer, promoting uniform mixing of the suspended phase and preventing local aggregation and solidification. The gradually expanding shape at the sample inlet induces a circumferential flow pattern in the initial stage, thereby exerting an outward ejection effect on the sample particles just entering the pipe, reducing the possibility of bottom-layer accumulation. These two design elements work together to significantly reduce the likelihood of adhesion and blockage problems traditionally associated with pipe design.
[0040] In one embodiment, such as Figure 2 As shown, the microchannel system 2 of the image flow cytometry detection device of this application has a spiral delivery structure on its inner wall. This structure consists of a guide vane 6 with a spiral angle of 45 degrees and a guide shaft 10. The spiral vane is installed on the inner surface of the microchannel system 2 and arranged at fixed intervals along its entire length, thereby forming a continuous spiral path. This design further guides the sample flow, making the fluid flow within the microchannel smoother and more efficient. At the same time, this spiral structure helps to reduce the probability of sample deposition during delivery, ensuring unobstructed flow. In particular, the guide shaft 10 is located at the core of the microchannel system 2, and its function is to provide a stable transport channel for the central fluid.
[0041] To maintain temperature stability during sample transport and prevent sample deterioration or sedimentation due to temperature differences, a heating rod 16 is installed inside the guide shaft 10. Specifically, the heating rod 16 is embedded in the hollow part of the guide shaft 10 and connected to an external controller to achieve precise temperature control. The operating status of the heating rod 16 can be monitored in real time by a built-in sensor and automatically adjusted according to preset values to maintain a constant temperature environment for the entire microchannel system 2. This ensures consistency of detection conditions and reduces the risk of sample variation.
[0042] For example, in the specific technical implementation process, the heating rod 16 is fixed inside the guide shaft 10 by a thermally conductive insulating material. At the same time, the guide shaft 10 and the microchannel system 2 adopt a seamless docking design to ensure effective transfer of heating effect and avoid heat loss. In addition, all components are assembled using highly compatible materials to ensure long-term stability and safety.
[0043] In one embodiment, see [specific example] Figure 1In this application, an anti-backflow valve 7 is installed at the junction of the sample inlet 1 and the microchannel system 2 of the image flow cytometry detection device. Specifically, the anti-backflow valve 7 is installed at the end of the flared, tapered inlet where it connects to the microchannel. This design prevents outside air and any contaminants that may be present from flowing back into the microchannel system 2 during sample introduction, thereby effectively improving the stability and cleanliness of the sample introduction stage.
[0044] Specifically, a flexible valve made of special materials with excellent sealing properties can be directly embedded into the junction of the converging inlet and the microchannel during the manufacturing stage. Under normal conditions, the valve is tightly closed to prevent any potential sources of contamination. When a sample is injected, the transient high pressure formed by the expansion of the inlet will cause the valve plate to open automatically, allowing the substance to flow smoothly into the tube. Once the sample is stopped, the valve will quickly return to its original state and close the channel, eliminating the possibility of external substances entering.
[0045] In one embodiment, see Figure 2 and Figure 3 The microchannel system 2 of the image flow cytometry detection device in this application is made of polycarbonate. This choice is primarily due to polycarbonate's high transparency, excellent mechanical strength, and good chemical resistance, ensuring no changes during long-term use and supporting high-quality optical signal capture. Furthermore, a low surface energy coating 8 is applied to the inner surface of the polycarbonate material to reduce the possibility of sample adhesion. By selecting a coating with appropriate properties, the sample can flow more smoothly through the microchannel system 2 during transport, without easily leaving residues or causing blockages.
[0046] Specifically, the construction of the microchannel system 2 involves selecting a specific type of polycarbonate for injection molding to form the desired structure, and then coating its inner surface with a low surface energy material using physical deposition or chemisorption. The low surface energy coating 8 can be uniformly applied to the entire inner surface using vapor deposition or solution impregnation. Specifically, after the polycarbonate channels are fabricated, they undergo pre-cleaning to remove contaminants, followed by coating with the appropriate low surface energy material according to the selected process. These steps ensure that the coating is not only robust and continuous but also minimizes the impact on sample flow, thereby guaranteeing an efficient and accurate sample analysis process.
[0047] In one embodiment, the illumination unit 3 of the image flow cytometry detection device of this application takes the form of a multi-LED array 9 (see [link]). Figure 1The light source is evenly distributed around the microchannel system 2 to achieve a balanced distribution and support high-quality imaging. Specifically, multiple LED components are arranged in an array around the microchannel, installed close to each other without affecting the normal passage of samples inside the microchannel, ensuring that each LED can effectively illuminate the entire area inside the microchannel. This design not only guarantees consistent light intensity but also avoids shadow problems, enabling ideal lighting conditions for image acquisition.
[0048] For example, the selection, arrangement, and angle of each LED are carefully calculated to create a uniform and sufficient illumination environment on the sides of the microchannel system 2. To achieve this, engineers need to adjust the parameters of the LED array 9, such as light intensity and color temperature, taking into account factors such as the microchannel size and sample flow rate, to match the requirements for clear sample microscopic photography under optimal operating conditions. This process may involve multiple simulations and testing verifications to ensure that the finally assembled equipment can operate stably and efficiently.
[0049] In one embodiment, the waste liquid outlet 5 of the image flow cytometry detection device of this application is connected to a self-priming pump 11 to quickly extract the sample liquid after detection, preventing backflow. This design avoids waste liquid stagnation at the end of the microchannel system 2, which could lead to secondary contamination or blockage. By placing the waste liquid outlet 5 at the very end of the microchannel system 2 and then connecting the self-priming pump 11 thereafter, it is ensured that the liquid can be discharged from the device in a timely and effective manner. The entire system can quickly return to its initial state after operation, ready for the next detection.
[0050] The specific self-priming pump 11 consists of two main parts: the pump body and the internally integrated self-priming mechanism. Together, they form a complete fluid delivery system. The pump body is made of corrosion-resistant materials, suitable for various types of cell samples and reagents. The device is also equipped with necessary valve components and connection interfaces. These structures work together to ensure unidirectional fluid flow and efficient system operation. For example, the pump body has an inlet and an outlet. The inlet directly connects to the waste liquid outlet 5, allowing waste liquid to flow smoothly into the pump and be discharged smoothly through subsequent channels, ensuring that backflow does not occur.
[0051] In one embodiment, continue to refer to Figure 1The microchannel system 2 of the image flow cytometry detection device of this application has a cleaning port 12 at its end. This port is precisely installed to ensure a seamless connection with the microchannel system 2 and is located at the final output section of the microchannel system 2. This design allows cleaning solvent to be periodically injected into the microchannel through the cleaning port 12, thereby thoroughly flushing the channel. To achieve efficient self-cleaning, the cleaning port 12 must be equipped with appropriate valve control mechanisms and connection structures. These structures prevent liquid backflow while ensuring that external solvents can smoothly enter and permeate the entire pipeline, thereby achieving the purpose of cleaning residues.
[0052] In practice, a cleaning interface 12 can be formed by directly installing an openable and closable valve at the end outlet of the micropipeline system 2. In use, the valve opens to allow the cleaning solution to flow along a predetermined path through a pump or other pressurizing device until it covers all contact surfaces, and then drains out of the system. This technique effectively supports online maintenance in automated operating procedures without disrupting or stopping daily operations.
[0053] The presence of cleaning interface 12 ensures the system's internal cleanliness and hygiene standards, and improves the efficiency of sample conversion between subsequent batches. This interface not only enhances system reliability but also simplifies maintenance procedures for operators. The cleaning process can be set to start periodically or on demand, tailored to the user's specific application. This ensures that detection accuracy is unaffected by contaminant contamination while allowing users to maintain optimal performance without disrupting experimental procedures.
[0054] In one embodiment, a flow monitoring sensor 13 at a key node is installed within the microchannel system 2 of the image flow cytometry detection device of this application (see [link]). Figure 1 These flow monitoring sensors 13 monitor the sample transport status in real time, ensuring that the microchannel remains unobstructed at all times. This not only avoids detection delays caused by sample blockage but also improves the operating efficiency and stability of the device. Specifically, the flow monitoring sensors 13 are deployed at multiple key locations in the microchannel system 2, covering critical flow sections from the flared inlet taper to the downstream end of the microchannel.
[0055] The selection and configuration of the flow monitoring sensor 13 takes into account its adaptability to environmental changes and its compatibility with biological samples. It employs a non-contact sensing principle, which does not cause additional interference to the flowing sample. The sensor can measure using optical, acoustic, or electromagnetic methods. During installation, these sensors are typically embedded in the outer wall of the microchannel and connected to a built-in data processing unit to ensure accurate signal acquisition and processing.
[0056] For example, in one specific implementation, the flow monitoring sensor 13 utilizes ultrasonic technology to monitor the fluid velocity within the microchannel in real time. These ultrasonic sensors, positioned near the exterior of the horn-shaped converging section, emit and receive sound wave signals reflected back from the microchannel wall, thereby accurately determining whether the sample flows smoothly. Simultaneously, the sensor is connected to the device's central control system, which can promptly issue an alarm and adjust sample input parameters when potential blockages are detected. This design not only improves the overall performance of the equipment but also makes troubleshooting faster and more convenient.
[0057] In one embodiment, such as Figure 3 As shown, the spiral groove structure of the image flow cytometry detection device of this application also includes multiple micro-protrusion units 14, which are uniformly distributed on the guide plate 6 to effectively disperse liquid pressure and reduce the probability of sample adhering to the wall. Specifically, the micro-protrusion units 14 are located on the inner surface of the spiral groove structure and are arranged at intervals on the guide plate 6. This arrangement ensures that the liquid sample can pass through the pipeline system more smoothly with the help of the micro-protrusion units 14, thereby reducing stagnation or blockage caused by excessive local pressure.
[0058] To further enhance sample transport efficiency, the guide vane 6 itself features a unique structural design. The spiral grooves not only provide a guiding path for liquid flow, but their optimized curvature and depth also minimize liquid resistance, ensuring a relatively stable flow pattern for the liquid sample throughout the channel. Furthermore, by precisely setting the number and spacing of the micro-protrusion units 14, problems caused by concentrated liquid pressure can be effectively mitigated. Therefore, the synergistic effect of the micro-protrusion units 14 and the spiral groove structure is fully realized during system operation.
[0059] For example, during the molding stage of the guide vane 6, the specific parameters of the micro-protrusion unit 14 can be pre-set based on the results of computational simulation, and the micro-protrusion unit 14 and the spiral groove can be cast together using a precision mold. The manufactured guide vane 6 is then installed into the microchannel system 2 to form a complete fluid control system. During operation, as the sample flows into this area, the micro-protrusion unit 14 immediately begins to function, helping to adjust the hydrodynamic properties and reduce unnecessary adhesion.
[0060] In one embodiment, see [specific example] Figure 4The image flow cytometry detection device of this application features a replaceable filter assembly 15 at the connection between the sample inlet 1 and the microchannel system 2. This filter assembly 15 is designed to intercept larger particles, preventing them from entering the microchannel system 2 and effectively avoiding blockage during sample flow. The filter assembly 15 is installed at the critical connection point between the sample inlet 1 and the microchannel system 2, ensuring that all incoming samples undergo preliminary filtration before entering the complex microchannel structure.
[0061] Specifically, the filter assembly 15 consists of a filter screen and its supporting structure. The filter screen is made of finely woven material, which can effectively capture particles larger than a preset standard. The filter assembly 15 is connected to the main body of the device by a snap-fit connection, which allows users to easily remove the old filter screen and replace it with a new one. The optimized design makes disassembly and assembly simple and reliable, and maintenance can be performed quickly without affecting the functions of other parts of the detection device.
[0062] For example, in practical applications, the sample is first introduced into the device through sample inlet 1. When the liquid carrying large particles flows through this inlet, a pre-installed replaceable filter blocks these large particles that are difficult to pass through, allowing only smaller particles suitable for transport by the microchannel system 2 to pass through and continue downstream into the spiral conveyor structure to begin the subsequent detection process. This not only improves sample preparation efficiency but also protects the core detection components from unnecessary damage.
[0063] In actual operation, when this device is in use, the sample to be tested can be introduced through sample inlet 1. After introduction, the sample enters the microchannel system 2, where the spiral conveying structure allows the sample to flow forward uniformly, reducing adhesion and blockage. Simultaneously, the funnel-shaped tapering design of the microchannels near sample inlet 1 induces eddies during sample flow, mitigating sample deposition. During this process, the illumination unit 3, installed on the side of the microchannel system 2, provides uniform illumination to the sample flowing through the channel, facilitating image acquisition. Subsequently, the sample continues to flow to the detection unit 4 on the downstream side of the microchannel system 2, where the optical signal is captured and processed. Finally, the liquid sample that has completed testing is discharged from the device through waste outlet 5. Because the microchannel system 2 is made of a transparent and smooth polymer material, this material is less prone to sample residue and supports high-definition imaging, making the entire process smooth and efficient.
[0064] The above are merely specific embodiments of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this application, and these should all be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
Claims
1. An image flow cytometry cell detection device, characterized in that, include: Sample inlet (1) is used to import the sample to be tested; A microchannel system (2) is used to transport a sample; wherein the microchannel system (2) is transparent, has a spiral transport structure on its inner wall, and the microchannel system (2) tapers in a funnel shape near the sample inlet (1); The illumination unit (3) is installed on the side of the microchannel system (2) to provide uniform illumination to the sample flowing through the microchannel system (2) in order to facilitate image acquisition; The detection unit (4), located on one side of the downstream section of the microchannel system (2), is used to capture the optical signal of the sample passing through the microchannel system (2); Waste liquid outlet (5), located at the end of the microchannel system (2), is used to discharge the liquid sample that has been tested; wherein The spiral conveying structure on the inner wall of the microchannel system (2) includes a guide plate (6) and a guide shaft (10), wherein a heating rod (16) is provided inside the guide shaft (10) to avoid sample deterioration or sedimentation caused by temperature difference changes; and The spiral conveying structure also includes multiple micro-protrusion units (14), which are evenly distributed on the guide plate (6).
2. The image flow cytometry cell detection device according to claim 1, characterized in that: The spiral conveying structure is a spiral-shaped concave structure.
3. The image flow cytometry cell detection device according to claim 2, characterized in that: The spiral angle of the guide vane (6) is 45 degrees.
4. The image flow cytometry cell detection device according to claim 3, characterized in that: The junction of the flared, tapering inlet (1) and the microchannel system (2) is equipped with an anti-reverse airflow valve (7).
5. The image flow cytometry cell detection device according to claim 1, characterized in that: The microchannel system (2) is coated with a low surface energy coating (8).
6. The image flow cytometry cell detection device according to claim 1, characterized in that: The lighting unit (3) is a multi-LED array (9).
7. The image flow cytometry cell detection device according to claim 1, characterized in that: The waste liquid outlet (5) is connected to a self-priming pump (11).
8. The image flow cytometry cell detection device according to claim 1, characterized in that: The end of the microchannel system (2) is provided with a cleaning port (12).
9. The image flow cytometry cell detection device according to claim 8, characterized in that: A flow monitoring sensor (13) is also installed in the micropipe system (2).
10. The image flow cytometry cell detection device according to claim 1, characterized in that: A replaceable filter assembly (15) is provided at the connection between the sample inlet (1) and the microchannel system.