Heat cover structure

Abstract

The utility model relates to a heat cover structure. The heat cover structure comprises a substrate, an optical fiber assembly and a heating plate, the substrate is provided with a mounting hole; the optical fiber assembly comprises an optical fiber and a lens connected with the optical fiber; a first through hole is formed in the heating plate and is aligned with the lens; a circulation groove is formed in the surface of the side, away from the lens, of the heating plate and connected with the first through hole. The circulation groove is used for communicating the first through hole with the side, away from the lens, of the heating plate. The circulating groove is formed in the side, away from the lens, of the heating plate, so that external air can be quickly exchanged and circulated with air in the circulating groove and the through hole, the temperature of the air in the circulating groove and the through hole is quickly increased, water mist on the lens can be quickly eliminated, interference on a light path transmitted by the lens is avoided, and the accuracy of a test result is improved.

Description

Technical Field

[0001] This application relates to the field of biodetection technology, and in particular to a heat-cap structure. Background Technology

[0002] In polymerase chain reaction (PCR) technology, the hot lid is a crucial component, primarily used to prevent the evaporation and condensation of liquids in the reaction system. PCR reactions typically require denaturation steps at high temperatures (e.g., 94-98°C), and the liquids in the reaction tubes are prone to evaporation at high temperatures, especially during prolonged cycles. Evaporation not only leads to changes in the volume of the reaction system but can also alter reactant concentrations, thereby affecting the efficiency and accuracy of PCR.

[0003] The design of the heated lid ensures that the liquid inside the reaction tube does not condense or evaporate due to temperature differences by providing a constant high-temperature surface at the top of the tube. Traditional heated lids are typically made of metal and maintain a constant temperature using heating elements, usually set at 100-110°C, slightly higher than the maximum temperature of the reaction system. This design effectively prevents liquid evaporation and avoids condensate flowing back into the reaction tube, ensuring the stability and reproducibility of the PCR reaction. With the development of PCR technology, the design of the heated lid is constantly being optimized.

[0004] Roche proposed a fiber-optic top-reading method, directly acquiring PCR fluorescence signals using optical fibers, thus eliminating the drawback of edge effects. However, its disadvantage lies in the direct detection at the top of the fiber, where the fiber is relatively far from the liquid, resulting in weaker light intensity and less stable signals. Later, some companies incorporated lenses into the fiber-optic top-reading structure. However, when the heated cap is heated, the air inside the chamber rapidly heats up, causing moisture in the air to condense on the lens surface, interfering with the light path transmitted through the lens and leading to signal attenuation or distortion. For experiments relying on optical detection (such as quantitative PCR), this means a significant reduction in data accuracy and reliability. Summary of the Invention

[0005] The purpose of this application is to provide a heat-cap structure that can improve the efficiency of disassembly and maintenance of the heat-cap structure, while eliminating fogging on the lens and improving the accuracy of the test.

[0006] This application provides a heat-cap structure, including:

[0007] The substrate has mounting holes;

[0008] An optical fiber assembly, comprising an optical fiber and a lens connecting the optical fiber; the lens is located within a mounting hole;

[0009] A heating plate is provided with a first through hole aligned with a lens; a flow groove is provided on the surface of the heating plate away from the lens, and the flow groove is connected to the first through hole; the flow groove is used to connect the first through hole and the side of the heating plate away from the lens.

[0010] In one embodiment, the heat-cap structure further includes:

[0011] A heat insulation plate is placed between the base plate and the heating plate.

[0012] The heat insulation plate is provided with a second through hole, which is connected to the first through hole of the heating plate.

[0013] In one embodiment, the heat-cap structure further includes:

[0014] A heating film is placed between a heat insulation plate and a heating plate to heat the heating plate.

[0015] The heating film is provided with a third through hole, which is connected to the second through hole and the first through hole.

[0016] In one embodiment, the heat-cap structure further includes:

[0017] A sealing gasket is placed between the heat insulation plate and the optical fiber assembly;

[0018] The sealing gasket is provided with a fourth through hole, which is connected to the third through hole, the second through hole and the first through hole.

[0019] In one embodiment, the heat-cap structure further includes:

[0020] The sealing ring has a first surface that contacts the substrate and a second surface that contacts the heating plate, and is used to seal the gap between the substrate and the heating plate.

[0021] In one embodiment, the optical fiber assembly includes:

[0022] A base with a through hole corresponding to the through hole; a lens aligned with the through hole is located on the side surface of the base near the heating plate.

[0023] The optical fiber passes through the through-hole to connect to the lens.

[0024] In one embodiment, the optical fiber assembly includes:

[0025] The lens clamp is located between the heating plate and the fiber optic assembly, and is in contact with the lens to fix it in place.

[0026] In one embodiment, the optical fiber assembly and the substrate are detachably connected.

[0027] In one embodiment, the heat cap structure further includes:

[0028] Fasteners are used to secure fiber optic assemblies and substrates.

[0029] In one embodiment, the heating plate includes a first plate and a second plate perpendicular to the first plate; a first through hole is disposed on the first plate; the second plate extends away from the substrate to form a heating space.

[0030] In one embodiment, the heat cap structure further includes:

[0031] The test tube is aligned with the first through hole and placed in the heating space. The opening diameter of the test tube is larger than the diameter of the first through hole.

[0032] In one embodiment, multiple first through holes are provided, and the multiple first through holes are located in the same flow groove.

[0033] In one embodiment, there are multiple first through holes and multiple flow grooves; the multiple first through holes are arranged in an array, and the first through holes in the same row are located in the same flow groove.

[0034] The advantages of this application compared to the prior art are:

[0035] This application provides a flow groove connected to the first through hole on the side of the heating plate away from the lens, allowing external air to quickly exchange and circulate with the air inside the flow groove and through hole. This can quickly eliminate water mist on the lens, avoid interference with the light path transmitted by the lens, and improve the accuracy and reliability of the test results. Attached Figure Description

[0036] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0037] Figure 1 This is a schematic diagram illustrating the working principle of a top-reading heated cover in related technologies.

[0038] Figure 2 This is a schematic diagram of a heat cover structure provided in an embodiment of this application;

[0039] Figure 3 This is a schematic diagram of the optical fiber assembly provided in the embodiments of this application;

[0040] Figure 4 This is a schematic diagram of the use of the heat cover structure provided in one embodiment of this application;

[0041] Figure 5 Provided in one embodiment of this application Figure 4 A magnified view of a portion of the image;

[0042] Figure 6 This is a schematic diagram of a heating plate structure provided in one embodiment of this application.

[0043] The above figures include the following reference numerals:

[0044] 1-Lens mounting plate; 2-Cavity; 3-Substrate; 4-Mounting hole; 5-Heating plate; 6-First through hole; 7-Flow groove; 8-First plate; 9-Second plate; 10-Heating space; 11-Fiber optic assembly; 12-Fiber optic cable; 13-Lens; 14-Base; 15-Through hole; 16-Lens pressure plate; 17-Heat insulation plate; 18-Second through hole; 19-Heating film; 20-Third through hole; 21-Sealing gasket; 22-Fourth through hole; 23-Sealing ring; 24-Test tube. Detailed Implementation

[0045] The terms “first,” “second,” “third,” etc., are used only for distinguishing descriptions and do not indicate a sequence number, nor should they be interpreted as indicating or implying relative importance.

[0046] Furthermore, terms such as "horizontal," "vertical," and "sag" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," not that the structure must be completely horizontal, but can be slightly tilted.

[0047] In the description of this application, it should be noted that the terms "inner", "outer", "left", "right", "upper", "lower", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this application is in use. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0048] In the description of this application, unless otherwise expressly specified and limited, the terms “set up,” “install,” “connect,” and “link” shall be interpreted broadly, for example, as a fixed connection, a detachable connection, or an integral connection; as a mechanical connection or an electrical connection; as a direct connection or an indirect connection through an intermediate medium; or as a connection within two components.

[0049] Figure 1 This is a schematic diagram illustrating the working principle of a top-reading heated cover in related technologies, such as... Figure 1As shown, the top-reading heated cover includes, from bottom to top in the illustrated direction, a heating plate 5, a heat insulation plate 17, and a lens mounting plate 1. The lens mounting plate 1 has multiple lenses 13. The heating plate 5 and the heat insulation plate 17 have multiple corresponding through holes, forming cavities 2. Each cavity 2 has a lens 13 on its upper surface and a test tube 24 on its lower surface. The upper surface of the test tube 24 contacts and tightly adheres to the lower surface of the heating plate 5, thus creating a sealed environment within the cavity 2 between the test tube 24 and the lens 13. When the heating plate 5 is heated, the hot air at the bottom of the cavity 2 rises (…). Figure 1 (The arrow indicates the direction) When the lens 13 on the upper side is exposed to fog, it will affect the entire optical path, causing abnormal light intensity signals. Furthermore, due to the small surface area of ​​the air being heated, the internal air is sealed, and the internal air heats up slowly, preventing moisture circulation and making it difficult to eliminate the fog. Additionally, mounting multiple lenses 13 on the lens mounting plate 1 is not only cumbersome, but also requires reinstalling all lenses 13 if an error occurs during operation. Moreover, if a lens 13 is damaged during later use, disassembly and maintenance will be extremely inconvenient.

[0050] Figure 2 This is a schematic diagram of a heat cover structure provided in an embodiment of this application, as shown below. Figure 2 As shown, this application provides a heat-covering structure, including: a substrate 3, an optical fiber assembly 11, and a heating plate 5. The substrate 3 has a mounting hole 4; the optical fiber assembly 11 includes an optical fiber 12 and a lens 13 connected to the optical fiber 12, the lens 13 being located within the mounting hole 4; the heating plate 5 is provided with a first through hole 6, the through hole being aligned with the lens 13; a flow groove 7 is provided on the surface of the heating plate 5 away from the lens 13, the flow groove 7 being connected to the first through hole 6; the flow groove 7 is used to connect the first through hole 6 and the side of the heating plate 5 away from the lens 13, wherein the heating plate 5 can be a heating aluminum plate.

[0051] This application provides a flow groove 7 on the side of the heating plate 5 away from the lens 13. The flow groove 7 is connected to the first through hole 6, which allows external air to exchange and circulate quickly with the air inside the flow groove 7 and the through hole. This can quickly eliminate water mist on the lens 13, which is set one-to-one with the first through hole 6, avoid interference with the light path transmitted by the lens 13, and improve the accuracy and reliability of the test results.

[0052] In one embodiment, the heat cover structure further includes: a heat insulation plate 17, a heating film 19, and a sealing gasket 21. The heat insulation plate 17 is disposed between the substrate 3 and the heating plate 5 to prevent the heat from the heating plate 5 from being directly conducted to the substrate 3, thereby reducing heat loss. The heating film 19 is disposed between the heat insulation plate 17 and the heating plate 5 to heat the heating plate 5. The sealing gasket 21 is disposed between the heat insulation plate 17 and the optical fiber assembly 11 to ensure a seal between the optical fiber assembly 11 and the heat insulation plate 17, preventing external air from entering the interior of the heat cover structure. The heat insulation plate 17 has a second through hole 18, the heating film 19 has a third through hole 20, and the sealing gasket 21 has a fourth through hole 22, which communicates with the third through hole 20, the second through hole 18, and the first through hole 6.

[0053] In one embodiment, Figure 3 This is a schematic diagram of the optical fiber assembly provided in the embodiments of this application, such as... Figure 3 As shown, the fiber optic assembly 11 includes: a base 14, multiple optical fibers 12, multiple lenses 13, and a lens clamping plate 16. The base 14 has multiple through holes 15 corresponding to the through holes; the lenses 13 are aligned with the through holes 15 and located on the side surface of the base 14 closest to the heating plate 5; the optical fibers 12 pass through the through holes 15 and connect to the lenses 13; the lens clamping plate 16 is located between the heating plate 5 and the fiber optic assembly 11, and contacts the lenses 13 for fixing the lenses 13.

[0054] In one embodiment, the heat cover structure further includes a fastener (not shown in the figure) for fixing the optical fiber assembly 11 and the substrate 3. The optical fiber assembly 11 and the substrate 3 are detachably connected by the fastener. In related technologies, multiple lenses 13 are uniformly mounted on the lens mounting plate 1 and combined with the heat cover. If an error occurs during operation, multiple lenses 13 will need to be reinstalled. Furthermore, if a lens 13 is damaged during later use, disassembly and maintenance are extremely inconvenient. The solution of this application is not only easy to install, but if the lens 13 and the optical fiber 12 are damaged, the entire optical fiber assembly 11 can be replaced individually, greatly saving maintenance time. The fastener can be a bolt, pin, or magnetic connector, etc.

[0055] In one embodiment, Figure 4 This is a schematic diagram of the use of the heat cover structure provided in one embodiment of this application. Figure 5 Provided in one embodiment of this application Figure 4 An enlarged schematic diagram of part I, as shown below. Figure 4-5As shown, the heated cover structure also includes: a test tube 24; a heating plate 5 including a first plate 8 and a second plate 9 perpendicular to the first plate 8; a first through hole 6 disposed on the first plate 8; the second plate 9 extending away from the substrate 3 to form a heating space 10; the test tube 24 is aligned with the first through hole 6 and disposed in the heating space 10, the opening diameter of the test tube 24 is larger than the diameter of the first through hole 6, the opening part of the test tube 24 contacts the heating plate 5, and the other part contacts the flow groove 7, wherein the optical fiber assembly 11, the sealing gasket 21, the heat insulation plate 17, the heating film 19, and the heating plate 5 are in close contact to ensure that the heat on the upper side of the test tube 24 will not be lost.

[0056] Specifically, the air inside the first through-hole 6 and the air outside (heating space 10) circulate directly. Although the air inside the first through-hole 6 will still form fog when it rises after being heated and encounters the lens 13 on the upper side, because the air in the first through-hole 6 and the heating space 10 circulate with each other, the air in the heating space 10 has a large heating area and heats up quickly, allowing for rapid exchange with the air inside the first through-hole 6. Figure 5 (As indicated by the arrow) This causes the air inside the first through-hole 6 to heat up rapidly, dissipating the water vapor and thus eliminating the fog. During the experiment, the light emitted from the optical fiber 12 in the optical fiber assembly 11 is focused by the lens 13, and then passes sequentially through the fourth through-hole 22, the second through-hole 18, the third through-hole 20, and the first through-hole 6 to irradiate the liquid in the test tube 24. The light emitted by the fluorescent dye in the liquid is then focused by the lens 13 back onto the optical fiber 12, and subsequently detected by the receiver.

[0057] In one embodiment, Figure 6 This is a schematic diagram of a heating plate structure provided in one embodiment of this application. Figure 6 As shown, multiple first through holes 6 are provided, and these first through holes 6 are all located within the same flow groove 7. With this design, external air (air within the heating space 10) can be evenly distributed within the flow groove 7, thereby ensuring the working efficiency and uniform heat conduction of each first through hole 6 of the heating plate 5.

[0058] In one embodiment, the heat cover structure further includes a sealing ring 23, the first surface of which contacts the substrate 3 and the second surface of which contacts the heating plate 5, and a second plate 9, for sealing the gap between the substrate 3 and the heating plate 5.

[0059] In one embodiment, such as Figure 6 As shown, there are multiple first through holes 6 and multiple flow channels 7; the multiple first through holes 6 are arranged in an array, and the first through holes 6 in the same row are located in the same flow channel 7, forming a more complex heat transfer network. This design not only improves the space utilization of the heating plate 5, but also enables a more balanced distribution of external air (air in the heating space 10) among different flow channels 7.

[0060] It should be noted that, where there is no conflict, the features in the embodiments of this application can be combined with each other. The above are merely preferred embodiments of this application and are not intended to limit this application. For those skilled in the art, this application can have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A heat-cap structure, characterized in that, include: A substrate having mounting holes; An optical fiber assembly, the optical fiber assembly including an optical fiber and a lens connecting the optical fiber; the lens is located within the mounting hole; A heating plate is provided with a first through hole aligned with the lens; a flow groove is provided on the surface of the heating plate away from the lens, the flow groove being connected to the first through hole; the flow groove is used to connect the first through hole and the side of the heating plate away from the lens.

2. The heat-cap structure according to claim 1, characterized in that, Also includes: A heat insulation plate is disposed between the substrate and the heating plate; The heat insulation plate is provided with a second through hole, which communicates with the first through hole of the heating plate.

3. The heat-cap structure according to claim 2, characterized in that, Also includes: A heating film is disposed between the heat insulation plate and the heating plate for heating the heating plate; The heating film is provided with a third through hole, which is connected to the second through hole and the first through hole.

4. The heat-cap structure according to claim 3, characterized in that, Also includes: A sealing gasket is disposed between the heat insulation plate and the optical fiber assembly; The sealing gasket is provided with a fourth through hole, which is connected to the third through hole, the second through hole and the first through hole.

5. The heat-cap structure according to claim 1, characterized in that, Also includes: A sealing ring, the first surface of which contacts the substrate and the second surface of which contacts the heating plate, is used to seal the gap between the substrate and the heating plate.

6. The heat-cap structure according to claim 1, characterized in that, The optical fiber assembly includes: A base having a through hole corresponding to the through hole; a lens aligned with the through hole and located on the side surface of the base near the heating plate; The optical fiber passes through the through-hole and connects to the lens.

7. The heat-cap structure according to claim 1, characterized in that, The optical fiber assembly includes: A lens clamping plate is located between the heating plate and the optical fiber assembly, and is in contact with the lens to fix the lens.

8. The heat-cap structure according to claim 1, characterized in that, The optical fiber assembly and the substrate are detachably connected.

9. The heat-cap structure according to claim 8, characterized in that, Also includes: Fasteners for securing the optical fiber assembly and the substrate.

10. The heat-cap structure according to claim 1, characterized in that, The heating plate includes a first plate and a second plate perpendicular to the first plate; the first through hole is disposed on the first plate; the second plate extends away from the substrate to form a heating space.

11. The heat-cap structure according to claim 10, characterized in that, Also includes: A test tube, aligned with the first through hole, is placed within the heating space, and the opening diameter of the test tube is larger than the diameter of the first through hole.

12. The heat-cap structure according to claim 1, characterized in that, The first through hole is provided in multiple ways, and the multiple first through holes are located in the same flow groove.

13. The heat-cap structure according to claim 1, characterized in that, The first through hole is provided in multiple ways, and the flow groove is provided in multiple ways; the multiple first through holes are arranged in an array, and the first through holes in the same row are located in the same flow groove.

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