Human recombinant triple-helix collagen culture device

Abstract

The utility model discloses a human-derived recombinant triple-helix collagen culture device which comprises a guide groove, a roller, a pressing plate, a hook lug, a long screw rod and a rotary pressing handle, according to the human-derived recombinant triple-helix collagen culture device, a culture box matched with a placement seat is arranged in the placement seat, guide grooves are symmetrically and fixedly connected to the two sides of the placement seat, rolling wheels are rotationally installed on the two sides of the culture box in parallel and correspond to the guide grooves, pressing plates are symmetrically hinged to the front side of the placement seat, and hook lugs are fixedly connected to the pressing plates; a long screw rod jointly penetrates through the notches, rotating pressing handles are in threaded connection with the two ends of the long screw rod, the culture box can be placed in the placement base in the mode that the rolling wheels are inserted into the guide grooves, meanwhile, the culture box can be limited at the front side opening of the placement base through closing of the pressing plates, and the long screw rod is matched with the rotating pressing handles to extrude the hook lugs to form fixation; therefore, an assembly type installation effect is formed, and the culture box can be conveniently taken out after the human-derived recombinant triple-helix collagen is cultured.

Description

Technical Field

[0001] This utility model relates to the field of recombinant humanized collagen culture technology, specifically a humanized recombinant triple helix collagen culture device. Background Technology

[0002] Collagen is a biological macromolecule with wide applications in biomedical materials, cosmetics, and health products. For a long time, the collagen sold on the market has primarily been animal-derived hydrolyzed collagen extracted from the skin and bones of pigs and cattle, or fish skin and scales, using acid, alkali, or enzymatic methods. However, with the outbreak of livestock diseases such as mad cow disease and the emergence of problems such as opacity, lack of standardization, and unreliable quality in the industrial production process of animal-derived hydrolyzed collagen, people have become concerned about the bioactivity and safety of animal collagen products. This has, to some extent, affected the rapid and orderly development of my country's collagen market. Recombinant human collagen is produced through industrial fermentation using protein engineering principles and molecular biology techniques, designed, synthesized, and constructed with methanol-inducible recombinant engineered bacteria. This human collagen, expressed through genetic engineering technology, solves various problems of traditional animal collagen products due to its well-defined molecular structure and bioactivity, as well as its non-antigenicity, and has broad application prospects and market potential.

[0003] A search revealed a patent document with publication number CN 221166569 U, which discloses a recombinant humanized collagen culture device. This device addresses the issues of low culture efficiency and limited practicality in related technologies. It includes an incubator and a temperature control device. A front door is rotatably mounted on one side of the incubator, and a partition is fixedly connected inside. A motor is installed on one side of the incubator. Through the arrangement of the motor and a straight rod, the motor drives a first connecting rod and a second connecting rod, causing the first and second culture boxes to shake slightly. This shaking increases the surface area for cell adhesion and growth, promoting better cell growth and reproduction. It also increases the exchange rate of oxygen and nutrients in the culture medium, helping cells better absorb nutrients and metabolites, maintaining normal cell growth and metabolism, and improving the practicality of the device.

[0004] While the existing technology can improve culture efficiency, the first and second culture boxes are directly penetrated by two rotating shafts, making it difficult to remove the culture boxes from the incubator and affecting the removal of the cultured products. Therefore, a new human recombinant triple helix collagen culture device is proposed to optimize the existing technology. Utility Model Content

[0005] The purpose of this invention is to provide a human recombinant triple helix collagen culture device to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A human recombinant triple-helix collagen culture device includes an incubator, characterized in that: two placement seats are arranged in parallel inside the incubator, the placement seats are rotatably installed with the incubator via a rotating shaft, a swing mechanism is provided on the outer side of the placement seats, a culture box adapted to it is provided inside the placement seats, guide grooves are symmetrically fixedly connected to both sides of the placement seats, and rollers are rotatably installed in parallel on both sides of the culture box corresponding to the guide grooves, the width of the rollers is adapted to the guide grooves, the front end of the guide groove is set with a flared opening, the flared opening facilitates the rollers to enter the guide groove, the front side and top surface of the placement seats are both set with openings, pressure plates are symmetrically hinged to the front side of the placement seats, hooks are fixedly connected to both pressure plates, the hooks are provided with notches, a long screw passes through the notches, the two ends of the long screw are threadedly connected to rotating handles, the rotating handles press against the hooks, and a nut corresponding to and adapted to the long screw is embedded through the center of the rotating handles, the rotating handles can be limited by the hooks of the hooks to prevent the rotating handles from detaching from the hooks.

[0008] As a further embodiment of this utility model: the swing mechanism includes a connecting rod, a horizontal groove, a toggle pin, a connecting pin, a turntable, and a drive motor.

[0009] As a further embodiment of this utility model: the connecting pin is rotatably connected to the side walls of the two placement seats and is vertically corresponding; the connecting rod is rotatably connected to the two connecting pins; the horizontal groove is fixedly connected to the bottom end of the connecting rod; the actuating pin is rotatably connected to the eccentric part of the turntable; the drive motor is fixedly installed on the side wall of the incubator; and the output end of the drive motor is inserted into the incubator and fixedly connected to the center of the turntable.

[0010] As a further improvement of this utility model: the actuating pin is inserted into the horizontal groove and is rolled in place.

[0011] As a further improvement of this utility model: the bottom of the incubator is provided with a support groove, which raises the entire incubator to facilitate the installation of the circulation ventilation pipe.

[0012] As a further embodiment of this utility model: a circulating ventilation pipe is fixedly connected to the rear side of the incubator, with both ends of the circulating ventilation pipe inserted into the top and bottom inner sides of the incubator, respectively. A circulating fan and an electric heating grid are fixedly installed inside the circulating ventilation pipe. Multiple temperature sensors are fixedly installed inside the incubator. An inspection port is provided on the circulating ventilation pipe corresponding to the circulating fan and the electric heating grid. An inspection cover is provided on the outer side of the inspection port. The inspection cover is assembled with the circulating ventilation pipe, which can be fixed by snap-fit ​​or bolt, preferably by bolt.

[0013] As a further improvement of this utility model: the front side of the incubator has a retrieval opening corresponding to the placement seat, and a matching door is provided on the outer side of the retrieval opening. One side of the door is hinged to the incubator, and a door lock is provided between the other side of the door and the incubator. The door lock is an existing cabinet lock available for sale.

[0014] As a further improvement of this invention: a control panel is fixedly installed on the outer wall of the incubator. The control panel has a built-in temperature control module. The drive motor, circulating fan, electric heating grid, and temperature sensor are all electrically connected to the control panel via wires. The temperature control module can set the temperature, and the operation of the electric heating grid is controlled by the temperature feedback from the temperature sensor, thus facilitating the control of the temperature inside the incubator. The control panel is connected to an external power source via a power module.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] 1. The culture box of this utility model can be placed into the placement seat by inserting rollers into the guide groove. At the same time, the culture box can be limited by the closing of the pressure plate through the front opening of the placement seat, and fixed by the long screw and the rotating pressure handle to squeeze the hook. This forms an assembled installation effect, so that the culture box can be easily removed after the human recombinant triple helix collagen culture is completed.

[0017] 2. This utility model uses a drive motor to drive a turntable to rotate. The turntable pushes a horizontal groove through a toggle pin. The horizontal groove, through a connecting rod and a connecting pin, pulls the culture box to make it shake slightly, which promotes better cell growth and reproduction. It can increase the exchange rate of oxygen and nutrients in the culture medium, help cells better absorb nutrients and metabolic products, and maintain the normal growth and metabolism of cells.

[0018] 3. This utility model uses a circulating fan in conjunction with a circulating ventilation pipe to circulate the air inside the incubator, thereby making it easier to maintain a uniform air temperature inside the incubator. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of a human recombinant triple-helix collagen culture device.

[0020] Figure 2 This is a cross-sectional view of a human recombinant triple-helix collagen culture device.

[0021] Figure 3 This is an internal diagram of a human recombinant triple-helix collagen culture device.

[0022] Figure 4 This is a partial structural illustration of a human recombinant triple-helix collagen culture device.

[0023] Figure 5This is an exploded view of a local structure in a human recombinant triple-helix collagen culture device.

[0024] In the diagram: 1. Incubator; 2. Placement seat; 3. Culture box; 4. Guide groove; 5. Roller; 6. Pressure plate; 7. Hook; 8. Long screw; 9. Rotating pressure handle; 10. Connecting rod; 11. Horizontal groove; 12. Actuating pin; 13. Connecting pin; 14. Turntable; 15. Drive motor; 16. Support groove; 17. Circulating ventilation pipe; 18. Circulating fan; 19. Electric heating grid; 20. Temperature sensor; 21. Inspection cover; 22. Chamber door; 23. Control panel. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Please see Figures 1-5 In this embodiment of the invention, a human recombinant triple-helix collagen culture device includes a culture chamber 1. Two placement seats 2 are arranged parallel to each other inside the culture chamber 1. The placement seats 2 are rotatably mounted to the culture chamber 1 via a rotating shaft. A swing mechanism is provided on the outer side of each placement seat 2. A culture box 3 adapted to the placement seat 2 is provided inside the placement seat 2. Guide grooves 4 are symmetrically fixedly connected to both sides of the placement seat 2. Rollers 5 are rotatably mounted parallel to each other on both sides of the culture box 3 corresponding to the guide grooves 4. The width of the rollers 5 is adapted to the width of the guide grooves 4. The front end of the guide groove 4 is a flared opening. The opening design facilitates the entry of the roller 5 into the guide groove 4. The front and top surfaces of the placement seat 2 are both open. The front of the placement seat 2 is symmetrically hinged with pressure plates 6. Each pressure plate 6 is fixedly connected with a hook lug 7. The hook lug 7 has a notch, and a long screw 8 passes through the notch. Both ends of the long screw 8 are threadedly connected to a rotating pressure handle 9. The rotating pressure handle 9 presses against the hook lug 7. A nut corresponding to and adapted to the long screw 8 is embedded in the center of the rotating pressure handle 9. The rotating pressure handle 9 can be limited by the hook of the hook lug 7 to prevent it from detaching from the hook lug 7.

[0027] The culture box 3 can be placed into the placement seat 2 by inserting the roller 5 into the guide groove 4. At the same time, the culture box 3 can be limited by the closing of the pressure plate 6 through the front opening of the placement seat 2, and the hook 7 can be squeezed by the long screw 8 in conjunction with the rotating pressure handle 9 to form a fixed position, thus forming an assembly installation effect. This allows the culture box 3 to be easily removed after the human recombinant triple helix collagen culture is completed.

[0028] The swing mechanism includes a connecting rod 10, a transverse groove 11, a toggle pin 12, a connecting pin 13, a turntable 14, and a drive motor 15.

[0029] Connecting pin 13 is rotatably connected to the side walls of the two placement seats 2 and is vertically aligned. Connecting rod 10 is rotatably connected to the two connecting pins 13. Horizontal groove 11 is fixedly connected to the bottom end of connecting rod 10. Actuating pin 12 is rotatably connected to the eccentric part of turntable 14. Drive motor 15 is fixedly installed on the side wall of incubator 1. After the output end of drive motor 15 is inserted into incubator 1, it is fixedly connected to the center of turntable 14.

[0030] The actuating pin 12 is inserted into the horizontal groove 11 and is rolled in place.

[0031] The turntable 14 is driven to rotate by the drive motor 15. The turntable 14 pushes the transverse groove 11 through the actuating pin 12. The transverse groove 11 pulls the culture box 3 slightly through the connecting rod 10 and the connecting pin 13, which promotes better cell growth and reproduction. It can increase the exchange rate of oxygen and nutrients in the culture medium, help cells better absorb nutrients and metabolic products, and maintain the normal growth and metabolism of cells. During cell culture, cells will form clumps, which will affect cell growth and differentiation. Shaking the culture box 3 can reduce the formation of cell clumps, make the cells more dispersed and evenly distributed, and improve the practicality of the device.

[0032] The bottom of the incubator 1 is provided with a support groove 16, which raises the incubator 1 as a whole, making it convenient to install the circulation ventilation pipe 17.

[0033] A circulating ventilation pipe 17 is fixedly connected to the rear side of the incubator 1. The two ends of the circulating ventilation pipe 17 are inserted into the top and bottom inner sides of the incubator 1, respectively. A circulating fan 18 and an electric heating grid 19 are fixedly installed inside the circulating ventilation pipe 17. Multiple temperature sensors 20 are fixedly installed inside the incubator 1. The circulating ventilation pipe 17 has an inspection port corresponding to the circulating fan 18 and the electric heating grid 19. An inspection cover plate 21 is provided on the outside of the inspection port. The inspection cover plate 21 is assembled with the circulating ventilation pipe 17. It can be fixed by snap-fit ​​or bolt, preferably bolted. Through the setting of the inspection cover plate 21 and the inspection port, Fang Baini can inspect and maintain the electric heating grid 19 and the circulating fan 18.

[0034] The incubator 1 has a retrieval opening on its front side corresponding to the placement seat 2. A door 22 is provided on the outer side of the retrieval opening. One side of the door 22 is hinged to the incubator 1, and a lock is provided between the other side of the door 22 and the incubator 1. The lock is an existing cabinet lock available for sale.

[0035] A control panel 23 is fixedly installed on the outer wall of the incubator 1. The control panel 23 has a built-in temperature control module. The drive motor 15, circulating fan 18, electric heating grid 19, and temperature sensor 20 are all electrically connected to the control panel 23 via wires. The temperature control module can set the temperature, and the operation of the electric heating grid 19 is controlled by the temperature feedback from the temperature sensor 20, which facilitates the control of the internal temperature of the incubator 1. The control panel 23 is connected to an external power supply via a power module.

[0036] The air inside the incubator 1 is circulated by the circulating fan 18 and the circulating ventilation pipe 17, which makes it easier to maintain a uniform air temperature inside the incubator 1.

[0037] The working principle of this utility model is as follows:

[0038] In use, open the chamber door 22, place the culture box 3 in the corresponding placement seat 2, so that the roller 5 is inserted into the guide groove 4, and then close the pressure plate 6. The pressure plate 6 presses into the culture box 3 to form a constraint. Further, the long screw 8 passes through the notch on the hook 7, and the rotating handle 9 is screwed on. Rotating the pressure handle 9 squeezes the hook 7 to form a compression constraint, so that the pressure plate 6 is relatively fixed. At this time, the chamber door 22 can be closed. Further, the temperature is set through the control panel 23. At the same time, the drive motor 15, the circulating fan 18, the electric heating grid 19, and the temperature sensor 20 are started. The control panel 23 controls the operation of the electric heating grid 19 through the temperature feedback of the temperature sensor 20. The circulating fan 18 circulates air through the circulating ventilation pipe 17, and the circulated air is heated by the electric heating grid 19. This maintains a constant internal temperature in incubator 1. The drive motor 15 rotates the turntable 14, which in turn pushes the transverse groove 11 via the actuating pin 12. The transverse groove 11, through the connecting rod 10 and connecting pin 13, pulls the placement seat 2 to swing. The placement seat 2 then causes the culture box 3 inside it to swing, resulting in slight shaking. This gentle shaking promotes better cell growth and reproduction, increases the exchange rate of oxygen and nutrients in the culture medium, and helps cells better absorb nutrients and metabolic products, maintaining normal cell growth and metabolism. During cell culture, cells can clump together, affecting cell growth and differentiation. Shaking the culture box 3 reduces cell clumping, making cells more dispersed and evenly distributed, improving the device's practicality. After culture is complete, the process can be reversed to remove the culture box 3 and extract the cultured human recombinant triple-helix collagen.

[0039] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A human recombinant triple-helix collagen culture device, comprising an incubator (1), characterized in that: The incubator (1) has two parallel placement seats (2) inside. The placement seats (2) are rotatably installed with the incubator (1) via a rotating shaft. The outer side of the placement seats (2) is provided with a swing mechanism. The placement seats (2) are provided with a culture box (3) that is adapted to them. The two sides of the placement seats (2) are symmetrically fixedly connected with guide grooves (4). The two sides of the culture box (3) are parallelly rotatably installed with rollers (5) corresponding to the guide grooves (4). The width of the rollers (5) is adapted to the guide grooves (4). The front end of the guide grooves (4) is set with a flared opening. The front side and top surface of the placement seats (2) are both set with openings. The front side of the placement seats (2) is symmetrically hinged with pressure plates (6). The pressure plates (6) are fixedly connected with hooks (7). The hooks (7) are provided with notches. A long screw (8) passes through the notches. The two ends of the long screw (8) are threadedly connected with rotating handles (9). The rotating handles (9) press against the hooks (7).

2. The human recombinant triple-helix collagen culture device according to claim 1, characterized in that: The swing mechanism includes a connecting rod (10), a transverse groove (11), a toggle pin (12), a connecting pin (13), a turntable (14), and a drive motor (15).

3. The human recombinant triple-helix collagen culture device according to claim 2, characterized in that: The connecting pin (13) is rotatably connected to the side walls of the two placement seats (2) and is vertically aligned. The connecting rod (10) is rotatably connected to the two connecting pins (13). The horizontal groove (11) is fixedly connected to the bottom end of the connecting rod (10). The actuating pin (12) is rotatably connected to the eccentric part of the turntable (14). The drive motor (15) is fixedly installed on the side wall of the incubator (1). After the output end of the drive motor (15) is inserted into the incubator (1), it is fixedly connected to the center of the turntable (14).

4. The human recombinant triple-helix collagen culture device according to claim 2, characterized in that: The actuating pin (12) is inserted into the transverse groove (11) and is in a rolling connection.

5. The human recombinant triple-helix collagen culture device according to claim 1, characterized in that: The bottom of the incubator (1) is provided with a support groove (16).

6. The human recombinant triple-helix collagen culture device according to claim 1, characterized in that: A circulating ventilation pipe (17) is fixedly connected to the rear side of the incubator (1). The two ends of the circulating ventilation pipe (17) are inserted into the top and bottom of the incubator (1) respectively. A circulating fan (18) and an electric heating grid (19) are fixedly installed inside the circulating ventilation pipe (17). Multiple temperature sensors (20) are fixedly installed inside the incubator (1). The circulating ventilation pipe (17) is provided with an inspection port corresponding to the circulating fan (18) and the electric heating grid (19). An inspection cover plate (21) is provided on the outside of the inspection port. The inspection cover plate (21) is assembled with the circulating ventilation pipe (17).

7. The human recombinant triple-helix collagen culture device according to claim 1, characterized in that: The incubator (1) has a pick-up and drop-off opening on the front side corresponding to the placement seat (2). A door (22) is provided on the outside of the pick-up and drop-off opening. One side of the door (22) is hinged to the incubator (1) by a hinge, and a door lock is provided between the other side of the door (22) and the incubator (1).

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