Planktonic microorganism sampling device

Abstract

The utility model relates to the technical field of plankton, in particular to a plankton sampling device which comprises a device main body, a filter box arranged at the top end in the device main body, a cover plate arranged at the top of the filter box, a multi-layer composite filter component arranged in the filter box, and a centrifugal component arranged at the bottom of one end of a two-way pipe, a liquid inlet is formed in the side wall of one side of the filter box and connected with a pump body, a transfer pipe is connected with a corrugated pipe, the corrugated pipe is connected with a sampling pipe, electric telescopic rods are symmetrically arranged at the top end of a connecting plate and connected with a support, the support is connected with an electric push rod, a filter plate is arranged in a fixing plate, and a waterproof pressure sensor is arranged on the fixing plate. A temperature sensor is arranged on one side of the waterproof pressure sensor, and the structure of the multi-layer composite filter assembly, the supporting frame, the stainless steel filter screen and the mounting plate is utilized, so that the problems that according to the structure, a single concentrated sample is obtained only through a centrifugal cylinder, an original water sample and a filtered water sample cannot be synchronously obtained, and contrast experiment data is lacked are solved.

Description

Technical Field

[0001] This utility model relates to the field of planktonic technology, specifically to a planktonic microorganism sampling device. Background Technology

[0002] Plankton refers to drifting organisms that live in water but lack effective mobility. It includes phytoplankton and zooplankton. Some plankton have the ability to swim, but their swimming speed is often slower than the ocean currents they are in, so they cannot move flexibly in the water. Among the organisms in oceans, lakes and rivers, plankton have no mobility at all, or only very weak mobility, so they cannot move against the current and instead float on the surface. These organisms are collectively called plankton, and sampling devices are needed in the detection of plankton.

[0003] Furthermore, the application document with publication number CN216473207U mentions a planktonic microorganism sampling device. In use, an external controller controls a rotary motor, a servo motor, and a water pump. The output of the servo motor drives a screw to rotate, which in turn moves a threaded sleeve downwards. The sleeve moves a connecting rod downwards, which in turn moves the sampling tube downwards, facilitating adjustment of the sampling height. After adjustment, the water pump generates suction at its input, drawing water through the sampling tube and corrugated pipe. The pump's output then delivers the water into the centrifuge chamber. The rotary motor's output, via a mounting base, drives the centrifuge to rotate, utilizing centrifugal force to sample the planktonic microorganisms. Separating organisms from water effectively improves sampling efficiency. The combined use of the above structures solves the problems of existing sampling devices being inconvenient to use, often requiring manual separation of water and plankton, and lacking the ability to adjust sampling height, thus reducing overall practicality and making them difficult for users. However, certain shortcomings still exist and need optimization. Specific shortcomings are as follows: This structure only separates plankton through a centrifuge tube, lacking a pretreatment filtration module. When the water contains a large amount of impurities, the centrifuge tube is prone to clogging, requiring frequent shutdowns for cleaning, reducing sampling continuity. Furthermore, it only obtains a single concentrated sample through the centrifuge tube, failing to simultaneously obtain raw and filtered water samples, and lacking control experimental data.

[0004] Therefore, it is particularly important to design a planktonic microbial sampling device to overcome the above-mentioned technical defects and improve its overall practicality. Utility Model Content

[0005] The purpose of this invention is to provide a planktonic microorganism sampling device to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A planktonic microorganism sampling device includes a main body, a filter box at the top of the main body, a cover plate at the top of the filter box, a multi-layer composite filter assembly inside the filter box, a double-pass pipe on the filter box, a centrifugal assembly at the bottom of one end of the double-pass pipe, a liquid inlet on one side wall of the filter box, the liquid inlet connected to a pump body, the liquid inlet of the pump body connected to a transfer pipe, the transfer pipe connected to a corrugated pipe, the corrugated pipe connected to a sampling tube, a connecting plate at the top of the sampling tube, symmetrically equipped with electric telescopic rods at the top of the connecting plate, the electric telescopic rods connected to a bracket, the bracket connected to an electric push rod, a fixing plate at the other end of the sampling tube, a filter plate inside the fixing plate, a waterproof pressure sensor on the fixing plate, and a temperature sensor on one side of the waterproof pressure sensor.

[0008] As a preferred embodiment of this utility model, the device body is provided with four movable wheels at the bottom corners, and the movable wheels are universal wheels with a self-locking structure. The device body is provided with an inspection door and a rotating shaft. The inspection door can rotate along the central axis of the rotating shaft, and a sealing gasket is provided on one side of the inspection door. The inspection door is fixed to the device body by a latch, and a push handle is provided on one side of the device body.

[0009] As a preferred embodiment of this utility model, the multi-layer composite filter assembly includes three sets of support frames. The interior of each of the three sets of support frames is respectively provided with a stainless steel filter screen, a nylon mesh, and a polytetrafluoroethylene membrane. The top of each support frame is provided with a mounting plate, and the cover plate is provided with a slot. The two ends of the slot and the filter box are respectively provided with mounting holes. The stainless steel filter screen has a pore size of 50μm, the nylon mesh has a pore size of 10μm, and the polytetrafluoroethylene membrane has a pore size of 0.45μm.

[0010] As a preferred embodiment of this utility model, the surface of the cover plate is provided with a plurality of grooves, the inside of the grooves is provided with a sealing gasket, and the bottom surface of the mounting plate is provided with a protrusion for matching the grooves, the protrusion and the grooves being fitted together.

[0011] As a preferred embodiment of this utility model, the centrifugal assembly includes a drive motor, a centrifugal cylinder, and a rotating ring. The inner side wall of the main body of the device is provided with a rotating groove for matching the rotating ring. The bottom end of the main body of the device is provided with a water outlet pipe. The top end of the centrifugal cylinder is provided with a feeding port. The top end of the feeding port is provided with a cover cylinder, and the cover cylinder and the feeding port are fixed in position by threads.

[0012] As a preferred embodiment of this utility model, the filter plate and the fixing plate are fixed in position by bolts, the transfer pipe is T-shaped, and the transfer pipe and the corrugated pipe are equipped with flow meters. The double-pass pipe, the outlet pipe and the transfer pipe are equipped with solenoid valves on their exteriors.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] This invention utilizes a planktonic microorganism sampling device with a multi-layer composite filter assembly, support frame, stainless steel filter screen, polytetrafluoroethylene membrane, nylon mesh, and mounting plate. The device is pushed to the sampling point and self-locked. An electric push rod drives the sampling tube to a designated depth. A pump then draws water, which passes through a front-end filter plate to intercept large impurities. The water sample is diverted to a transfer pipe to obtain the original sample. The remaining water sample is filtered through a three-stage filter cartridge. The final filtrate enters a centrifuge assembly for concentration via a dual-pass pipe. Simultaneously, the original water sample, filtered water sample, and concentrated sample are obtained for comparison. The modular design supports customization of filter cartridge pore size and material. The support frame can be independently disassembled for sample transfer. This design solves the problems of previous structures that only separate planktonic organisms through a centrifuge tube, lacking a pretreatment filtration module. When the water contains a large amount of impurities, the centrifuge tube is prone to clogging, requiring frequent shutdowns for cleaning and reducing sampling continuity. Furthermore, it only obtains a single concentrated sample through the centrifuge tube, failing to simultaneously obtain original and filtered water samples, resulting in a lack of control experimental data. Attached Figure Description

[0015] Figure 1 This is a planar structural diagram of the present invention;

[0016] Figure 2 This is a schematic diagram of the filter box structure of this utility model;

[0017] Figure 3 This is a structural schematic diagram of the sampling pipeline assembly layout of this utility model.

[0018] In the diagram: 1. Main body of the device; 101. Filter box; 102. Cover plate; 104. Dual-way pipe; 105. Liquid inlet; 106. Pump body; 108. Corrugated pipe; 109. Sampling tube; 110. Connecting plate; 111. Electric telescopic rod; 112. Bracket; 113. Electric push rod; 114. Fixing plate; 115. Filter plate; 116. Waterproof pressure sensor; 117. Temperature sensor; 118. Transfer pipe; 2. Multi-layer composite filter assembly; 201. Support frame; 202. Stainless steel filter screen; 203. Polytetrafluoroethylene membrane; 204. Nylon mesh; 205. Mounting plate; 206. Groove; 207. Mounting hole; 3. Centrifuge assembly. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0020] To facilitate understanding of this utility model, a more comprehensive description will be given below with reference to the accompanying drawings. Several embodiments of this utility model are provided. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of this utility model will be more thorough and complete.

[0021] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or there may be an intervening component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there may be an intervening component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] For examples, please refer to Figure 1-3 This utility model provides a technical solution:

[0024] A planktonic microorganism sampling device includes a main body 1. A filter box 101 is located at the top of the main body 1, and a cover plate 102 is located on the top of the filter box 101. A multi-layer composite filter assembly 2 is located inside the filter box 101. A double-pass pipe 104 is provided on the filter box 101, and a centrifugal assembly 3 is located at the bottom of one end of the double-pass pipe 104. A liquid inlet 105 is located on one side wall of the filter box 101, and the liquid inlet 105 is connected to a pump body 106. The liquid inlet of the pump body 106 is connected to a transfer pipe 118, and the transfer pipe 118 is connected to a corrugated pipe 108. The corrugated pipe 108 is connected to a sampling tube 109, and a connecting plate 110 is located at the top of the sampling tube 109. Electric motors are symmetrically mounted on the top of the connecting plate 110. Telescopic rod 111, electric telescopic rod 111 connected to bracket 112, bracket 112 connected to electric push rod 113, sampling tube 109 has a fixed plate 114 at the other end, fixed plate 114 has a filter plate 115 inside, fixed plate 114 has a waterproof pressure sensor 116 on the fixed plate 114, and a temperature sensor 117 on one side of the waterproof pressure sensor 116. Sampling cups are placed at one end of transfer tube 118 and double-pass tube 104 respectively. The main body 1 of the device is pushed to a suitable position by the pusher, and then the moving wheel is self-locked by the self-locking structure to fix the position of the device. Then the electric push rod 113 is activated to push the bracket 112 to a suitable position horizontally. At this time, the sampling tube 109 is located at the top of the water surface. Afterwards, the electric telescopic rod 111 is activated to drive the sampling tube 109 into the water. The waterproof pressure sensor 116, in conjunction with the temperature sensor 117, monitors the diving depth in real time and provides feedback for adjustment. Once the designated depth is reached, the pump body 106 is activated to pump water for sampling. The filter plate 115 is located at the end of the sampling tube 109 closest to the water resource and is used to filter large-volume impurities such as snail shells. The water resource enters the bellows 108 through the sampling tube 109. Part of the water resource enters one end of the transfer pipe 118 and, through the cooperation of a flow meter and a solenoid valve, after obtaining the original water sample of the designated volume, one end of the transfer pipe 118 is closed. The remaining water resource enters the pump body 106 and is delivered to the filter box 101. The filter box 101 is then processed according to... Taking the target biological sample as a virus or nanoscale plankton as an example, the multi-layer composite filter component 2 can be assembled. The stainless steel filter 202 intercepts large cladocerans and fish eggs, the nylon mesh 204 intercepts adult copepods and rotifers, and the polytetrafluoroethylene membrane 203 intercepts diatoms, small dinoflagellates, and bacteria. The remaining viruses and nanoscale plankton can be centrifuged and concentrated through the double-pass tube 104 into the centrifuge component 3. The filter element and pore size of the multi-layer composite filter component 2 can be customized according to actual needs. Each support frame 201 can be disassembled and transferred to extract the target sample. At the same time, a filtered water sample can be obtained at the other end of the double-pass tube 104. The three sets of water samples can be compared, and the waste liquid is discharged through the outlet pipe.

[0025] The main body 1 of the device has four casters at its bottom corners, and these casters are omnidirectional with a self-locking mechanism. The main body 1 also has an inspection door. The omnidirectional wheels and self-locking mechanism allow the device to move flexibly and securely in complex terrain, improving field operation efficiency. The main body 1 has a pivot, and the inspection door can rotate along the central axis of the pivot. One side of the inspection door has a sealing gasket, and the inspection door is fixed to the main body 1 by a latch. The inspection door uses a pivot sealing design, which facilitates quick and easy maintenance of internal components while ensuring the device's airtightness. To prevent contamination, a push handle is provided on one side of the main body 1. The multi-layer composite filter assembly 2 includes three sets of support frames 201. The interiors of the three support frames 201 are respectively equipped with a stainless steel filter screen 202, a nylon mesh 204, and a polytetrafluoroethylene membrane 203. A mounting plate 205 is provided at the top of the support frame 201, and a slot 206 is provided on the cover plate 102. Mounting holes 207 are provided at both ends of the slot 206 and on the filter box 101. The stainless steel filter screen 202 has a pore size of 50 μm, the nylon mesh 204 has a pore size of 10 μm, and the polytetrafluoroethylene membrane 203 has a pore size of 0 μm. The 0.45μm modular filter cartridge design facilitates quick and easy assembly, replacement, and disassembly. It retains target biological samples while removing other non-target samples, adapting to different water quality sampling needs. The surface of the cover plate 102 has several grooves, with sealing gaskets inside the grooves. The bottom surface of the mounting plate 205 has protrusions that fit into the grooves, and the protrusions and grooves interlock. The sealing design between the top mounting plate of the support frame and the grooves of the cover plate ensures that there is no leakage or contamination during the filtration process. The centrifuge assembly 3 includes a drive motor, a centrifuge cylinder, and a rotating ring. The inner side wall of the main body 1 has a design for... The rotating ring is adapted to the rotating trough. The bottom of the main body 1 of the device is equipped with a water outlet pipe. The top of the centrifuge tube is equipped with a feeding port. The top of the feeding port is equipped with a cover. The cover and the feeding port are fixed by threads to facilitate the acquisition of the target sample centrifuged liquid. The filter plate 115 and the fixing plate 114 are fixed by bolts. The transfer tube 118 is T-shaped. The transfer tube 118 and the corrugated pipe 108 are equipped with flow meters. The double-pass pipe 104, the water outlet pipe and the transfer tube 118 are equipped with solenoid valves to facilitate the acquisition of the original water sample, the filtered water sample and the concentrated sample for reference.

[0026] The working process of this utility model is as follows: When using this planktonic microorganism sampling device, firstly, sampling cups are placed at one end of the transfer pipe 118 and the double-pass pipe 104, respectively. The main body 1 of the device is pushed to a suitable position using a pusher. Then, the self-locking structure is used to lock the moving wheels and fix the position of the device. Subsequently, the electric push rod 113 is activated to push the bracket 112 to a suitable position horizontally. At this time, the sampling tube 109 is located at the top of the water surface. Then, the electric telescopic rod 111 is activated to drive the sampling tube 109 into the water. The waterproof pressure sensor 116, together with the temperature sensor 117, monitors the diving depth in real time and provides feedback for adjustment. After reaching the designated depth, the pump body 106 is activated to pump water for sampling. The filter plate 115 is located at the end of the sampling tube 109 near the water resource and is used to filter large-volume impurities such as snail shells. The water resource enters the corrugated pipe 108 through the sampling tube 109, and part of the water resource enters one end of the transfer pipe 118 through the flow meter. In conjunction with the solenoid valve, after obtaining the original water sample of the specified volume, one end of the transfer pipe 118 is closed, and the remaining water resources enter the pump body 106 and are transported to the filter box 101. The filter box 101 can be equipped with multi-layer composite filter components 2 according to the requirements, taking the target biological sample as a virus or nano-scale plankton as an example. Among them, the stainless steel filter screen 202 intercepts large cladocerans, fish eggs, etc., the nylon mesh 204 intercepts adult copepods and rotifers, and the polytetrafluoroethylene membrane 203 intercepts diatoms, small dinoflagellates, and bacteria. The remaining viruses and nano-scale plankton can enter the centrifuge component 3 through the double-pass pipe 104 for centrifugation and concentration. The filter element and pore size of the multi-layer composite filter component 2 can be customized according to actual needs. Each support frame 201 can be disassembled and transferred to extract the target sample. At the same time, the filtered water sample can be obtained at the other end of the double-pass pipe 104. The three sets of water samples can be compared, and the waste liquid is discharged through the outlet pipe.

[0027] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

[0028] All standard parts used in this application can be purchased from the market. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art. The control method is through a controller, which is a PLC controller. At the same time, the two terminals of the external controller are connected to a power plug through wires. In this application, the mains power is used for power supply. The control circuit of the controller can be implemented by a person skilled in the art through simple circuit connection. It is common knowledge in the art. Therefore, this application will not explain the control method and circuit connection in detail.

Claims

1. A planktonic microorganism sampling device, comprising a device body (1), characterized in that: The main body (1) of the device has a filter box (101) at its top. The top of the filter box (101) has a cover plate (102). The filter box (101) has a multi-layer composite filter assembly (2) inside. The filter box (101) has a double-pass pipe (104). One end of the double-pass pipe (104) has a centrifugal assembly (3). One side wall of the filter box (101) has a liquid inlet (105). The liquid inlet (105) is connected to a pump body (106). The liquid inlet of the pump body (106) is connected to a transfer pipe (118). The transfer pipe (118) is connected to a corrugated pipe (108). (108) Connect the sampling tube (109). The top end of the sampling tube (109) is provided with a connecting plate (110). The top end of the connecting plate (110) is symmetrically provided with an electric telescopic rod (111). The electric telescopic rod (111) is connected to a bracket (112). The bracket (112) is connected to an electric push rod (113). The other end of the sampling tube (109) is provided with a fixing plate (114). The inside of the fixing plate (114) is provided with a filter plate (115). The fixing plate (114) is provided with a waterproof pressure sensor (116). A temperature sensor (117) is provided on one side of the waterproof pressure sensor (116).

2. The planktonic microorganism sampling device according to claim 1, characterized in that: The device body (1) has four corners at the bottom of the device body, and the four corners of the device body are provided with casters. The casters are provided with a self-locking structure. The device body (1) is provided with an inspection door. The device body (1) is provided with a rotating shaft. The inspection door can rotate along the central axis of the rotating shaft. The inspection door is provided with a sealing gasket on one side. The inspection door is fixed to the device body (1) by a latch. The device body (1) is provided with a push handle on one side.

3. The planktonic microorganism sampling device according to claim 1, characterized in that: The multi-layer composite filter assembly (2) includes three sets of support frames (201). The interior of each of the three sets of support frames (201) is provided with a stainless steel filter screen (202), a nylon mesh (204), and a polytetrafluoroethylene membrane (203). The top of each support frame (201) is provided with an mounting plate (205), and the cover plate (102) is provided with a slot (206). The two ends of the slot (206) and the filter box (101) are provided with mounting holes (207). The stainless steel filter screen (202) has a pore size of 50 μm, the nylon mesh (204) has a pore size of 10 μm, and the polytetrafluoroethylene membrane (203) has a pore size of 0.45 μm.

4. The planktonic microorganism sampling device according to claim 3, characterized in that: The surface of the cover plate (102) is provided with a plurality of grooves, and the inside of the grooves is provided with sealing gaskets. The bottom surface of the mounting plate (205) is provided with protrusions for matching the grooves, and the protrusions are fitted into the grooves.

5. The planktonic microorganism sampling device according to claim 1, characterized in that: The centrifugal assembly (3) includes a drive motor, a centrifugal cylinder, and a rotating ring. The inner side wall of the main body (1) is provided with a rotating groove for matching the rotating ring. The bottom end of the main body (1) is provided with a water outlet pipe. The top end of the centrifugal cylinder is provided with a feeding port. The top end of the feeding port is provided with a cover cylinder. The cover cylinder and the feeding port are fixed in position by threads.

6. A planktonic microbial sampling device according to claim 1 or 5, characterized in that: The filter plate (115) and the fixing plate (114) are fixed in position by bolts. The transfer pipe (118) is T-shaped and a flow meter is provided on the transfer pipe (118) and the corrugated pipe (108). Solenoid valves are provided on the outside of the double pipe (104), the water outlet pipe and the transfer pipe (118).

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