Object stage living cell in-vitro culture device

Abstract

The utility model discloses an objective table living cell in-vitro culture device, and relates to the technical field of mytilus edulis blood cell in-vitro culture. The culture device comprises a culture device body, a heating plate, a top cover, a partition plate, a thin slide and a metal sheet, an adjusting mechanism is arranged on the lower portion of the interior of the culture device body, a screw is arranged on the other side of a movable plate, and a rotating rod is arranged on the screw. The moving plates are adjusted, so that the positions of the two groups of moving plates are changed, the moving plates are matched with the size of a cell culture plate which can be placed in the settling tank, the maximum size of the cell culture plate is the size of the settling tank, and the moving plates are smoothly collected into the storage tank under the action of the screw rod at the moment, so that the culture device main body is convenient to use in the use process. The culture device can flexibly adapt to cell culture plates with multiple sizes, so that the use flexibility of the culture device main body is improved.

Description

Technical Field

[0001] This utility model relates to the field of in vitro culture technology of mussel blood cells, specifically a live cell in vitro culture device on a platform. Background Technology

[0002] Living cells are the basic structural and functional units of living organisms, so real-time observation of individual living cells is of great significance. In order to observe cell activity, migration and proliferation over a long period of time, a cell culture device that matches the microscope stage mechanism is required. When culturing mussel blood cells in vitro, a live cell in vitro culture device with a stage is needed.

[0003] A search revealed that Chinese patent number CN214612535U discloses a "live cell in vitro culture device with a stage." The design of the U-shaped spacer allows for a smoother gas injection process and better thermal uniformity within the culture chamber. The convective heat transfer effect caused by gas flow improves heat transfer efficiency and uniformity. However, this live cell in vitro culture device requires the use of standardized cell culture plates. This necessitates that laboratories either order specific sizes from the manufacturer or prepare cell culture plates that meet the device's requirements. Consequently, the device lacks flexibility in practical use, making it unsuitable for most laboratories. Utility Model Content

[0004] Based on this, the purpose of this utility model is to provide a live cell in vitro culture device with a stage to solve the technical problem that the limiting part of the existing live cell in vitro culture device with a stage has a fixed size and the flexibility in use needs to be further improved.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a live cell in vitro culture device with a stage, comprising a culture device body, a heating plate, a top cover, a partition, a thin glass slide, and a metal sheet. An adjustment mechanism is provided at the lower interior of the culture device body. The adjustment mechanism includes a settling tank, a storage tank, and a moving plate. A gasket is provided on one side of the moving plate, and a screw is provided on the other side of the moving plate. A rotating rod is provided on the screw. A wire outlet is provided on the front side of the culture device body, and a sensor is provided on one side of the wire outlet. An air inlet is provided on the rear side of the culture device body, and a vent is provided on the partition.

[0006] By adopting the above technical solution and adjusting the setting of the mechanism, the edge of the sedimentation tank where the cell culture plate is placed can be adjusted, so that the moving plate and the pad can be smoothly locked around the cell culture plate, and the cell culture plate of different sizes can be limited.

[0007] Furthermore, the movable plate and the storage slot are provided in two sets, one set of the movable plate is adapted to one set of storage slots, and the other set of the movable plate is adapted to another set of storage slots.

[0008] By adopting the above technical solution, one set of movable plates and storage tanks are set on the left and right sides, and another set of movable plates and storage tanks are set on the front and rear sides, so as to use the two sets of movable plates to limit the cell culture plates placed in the sedimentation tank.

[0009] Furthermore, the settling trough has a rectangular structure and is connected to the storage trough.

[0010] By adopting the above technical solution, the sedimentation tank can be used to place cell culture plates. The rectangular sedimentation tank limits the cell culture plates around their perimeter. If the size of the cell culture plates is smaller than the sedimentation tank, the adjustment mechanism can be activated.

[0011] Furthermore, the screw is threadedly connected to the main body of the culture device, the screw is fixedly connected to the rotating rod, and the screw is rotatably connected to the moving plate.

[0012] By adopting the above technical solution, when the rotating rod is manually rotated, it drives the screw fixedly connected to it to rotate, so that the screw continues to extend into or out of the main body of the culture device, thereby changing the position of the moving plate.

[0013] Furthermore, the partition has a U-shaped structure, and the ventilation holes are provided in two sets, with multiple ventilation holes in each set, and the multiple ventilation holes are evenly and equidistantly distributed.

[0014] By adopting the above technical solution, the U-shaped partition and the two sets of vent holes allow the gas entering from the air inlet to first pass through the space between the partition and the main body of the culture device, be heated by the heating plate, and then enter the partition through the vent holes.

[0015] Furthermore, the heating plate is provided in two sets, which are arranged symmetrically. A heat insulation pad is provided on the outer side of the heating plate, and the heat insulation pad is a hollow rectangular structure with one side open.

[0016] By adopting the above technical solution, two sets of symmetrically arranged heating plates can heat the airflow between the partition and the main body of the culture device, so that the incoming gas reaches the most suitable temperature for the growth of mussel blood cells.

[0017] Furthermore, a limiting groove is provided on the top of the main body of the culture device, a first through groove and a first locking groove are provided on the inner side of the bottom of the main body of the culture device, a limiting block is provided on the bottom of the top cover, a second through groove and a second locking groove are provided on the top cover, a top edge and a top locking strip are provided on the top of the partition, and a bottom edge and a bottom locking strip are provided on the bottom of the partition.

[0018] By adopting the above technical solution, the bottom edge and bottom clip extend into the first through groove and the first clip groove, and the top edge and top clip extend into the second through groove and the second clip groove, thereby making the side of the partition near the air inlet form a relatively sealed space with the main body of the culture device and the top cover.

[0019] Furthermore, fixing plates are provided on both sides of the top cover, the fixing plates are fixedly connected to the top cover, and the top cover is fixedly connected to the limiting block.

[0020] By adopting the above technical solution, the setting of the fixing plate makes it convenient for the hands to apply force on both sides of the top cover when removing the top cover, so that the top cover can be separated from the main body of the culture device and the partition.

[0021] Furthermore, the limiting blocks are provided in two sets, with two limiting blocks in each set. The two limiting blocks are arranged symmetrically, and the limiting blocks engage with the limiting grooves.

[0022] By adopting the above technical solution, each set of two symmetrical limiting blocks can engage with the limiting groove at multiple positions on the bottom of the top cover.

[0023] Furthermore, both the first and second through slots are U-shaped structures, the first and second through slots are consistent, the first and second through slots are adapted to the top and bottom edges, the first and second locking slots are consistent, and the first and second locking slots engage with the top locking strip and the bottom locking strip.

[0024] By adopting the above technical solution, the top edge and bottom edge can be smoothly inserted into the second through groove and the first through groove, respectively, and the top locking strip and the bottom locking strip can be smoothly inserted into the second locking groove and the first locking groove, respectively.

[0025] In summary, the present invention has the following main advantages:

[0026] 1. This utility model, by setting up a storage trough, a movable plate, and a screw, uses the rotation of the screw to adjust the movable plate, thereby changing the position of the two sets of movable plates to adapt to the size of the cell culture plates that can be placed in the sedimentation tank. The maximum size of the cell culture plate is the size of the sedimentation tank. At this time, the movable plate is smoothly retracted into the storage trough under the action of the screw. In this way, the main body of the culture device can flexibly adapt to cell culture plates of various sizes during use, thereby improving the flexibility of the main body of the culture device.

[0027] 2. This utility model, by setting a top cover, limiting block, partition, top edge, top locking strip, bottom edge, and bottom locking strip, uses the partition to divide the internal space of the main body of the culture device into two parts. The combination of the top edge, top locking strip, bottom edge, and bottom locking strip with the second through groove, the second slot, the first through groove, and the first slot respectively ensures that the connection between the partition on the air inlet side and the top cover and the main body of the culture device is seamless. This ensures that the gas entering must be heated in the space outside the partition before entering the inside of the partition. At the same time, the presence of the limiting block, top edge, top locking strip, bottom edge, and bottom locking strip makes the installation and disassembly of the top cover and the partition simpler and faster. Attached Figure Description

[0028] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0029] Figure 2 This is a rear-view three-dimensional structural diagram of the present invention;

[0030] Figure 3 This is a bottom-view three-dimensional structural diagram of the present invention;

[0031] Figure 4 This is a top-view three-dimensional structural diagram of the present invention;

[0032] Figure 5 This utility model Figure 4 Enlarged structural diagram at point A in the middle.

[0033] In the diagram: 1. Main body of the culture device; 2. First through groove; 3. First slot; 4. Limiting groove; 5. Heating plate; 6. Heat insulation pad; 7. Top cover; 8. Limiting block; 9. Second through groove; 10. Second slot; 11. Partition; 12. Top edge; 13. Top retaining strip; 14. Bottom edge; 15. Bottom retaining strip; 16. Vent hole; 17. Adjustment mechanism; 170. Settling tank; 171. Storage tank; 172. Moving plate; 173. Screw; 174. Rotating rod; 175. Gasket; 18. Air inlet; 19. Sensor; 20. Wire outlet; 21. Thin glass slide; 22. Fixing plate; 23. Metal sheet. Detailed Implementation

[0034] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0035] The embodiments of this utility model will be described below based on its overall structure.

[0036] Example 1:

[0037] A staged live cell in vitro culture device, such as Figures 1-5 As shown, the device includes a culture apparatus body 1, a heating plate 5, a top cover 7, a partition 11, a thin glass sheet 21, and a metal sheet 23. An adjustment mechanism 17 is located at the lower interior of the culture apparatus body 1. The adjustment mechanism 17 includes a settling tank 170, a storage tank 171, and a moving plate 172. A gasket 175 is located on one side of the moving plate 172, and a screw 173 is located on the other side. A rotating rod 174 is mounted on the screw 173. A wire outlet 20 is located on the front side of the culture apparatus body 1, and a sensor 19 is located on one side of the wire outlet 20. An air inlet 18 is located on the rear side of the culture apparatus body 1. An opening is located on the partition 11. With the inclusion of vents 16 and an adjustment mechanism 17, the edges of the settling trough 170 for placing cell culture plates can be adjusted, allowing the moving plate 172 and the pad 175 to be smoothly engaged around the cell culture plates. This limits the positioning of cell culture plates of different sizes, thereby improving the flexibility of the entire culture device 1 during use. Furthermore, the adjustment process of the screw 173 ensures that the moving plate 172 and the pad 175 can stop at any position at any time, and that they can immediately maintain their position after stopping. A sealing gasket is provided at the wire outlet 20 to increase the sealing performance of the wire outlet 20.

[0038] See Figure 4 Two sets of movable plates 172 and storage troughs 171 are provided. One set of movable plates 172 is adapted to one set of storage troughs 171, and the other set of movable plates 172 is adapted to another set of storage troughs 171. One set of movable plates 172 and storage troughs 171 is located on the left and right sides, and the other set of movable plates 172 and storage troughs 171 is located on the front and rear sides. This is so that the two sets of movable plates 172 can be used to limit the cell culture plates placed in the sedimentation tank 170. When the movable plates 172 move outward to their limit, they can be retracted into the corresponding storage troughs 171, so that the maximum size of the cell culture plates placed in the sedimentation tank 170 is consistent with the size of the sedimentation tank 170.

[0039] See Figure 4 , Figure 5 The settling trough 170 has a rectangular structure and is connected to the placement trough 171. The settling trough 170 can be used to place cell culture plates. The rectangular settling trough 170 is used to limit the cell culture plates around their perimeter. If the size of the cell culture plates is smaller than the settling trough 170, the adjustment mechanism 17 can be activated to use the moving plate 172 to assist in limiting the position. The settling trough 170 and the placement trough 171 are kept in a connected state, so that the maximum position of the moving plate 172 moving outward is inside the placement trough 171.

[0040] See Figure 1 , Figure 2 , Figure 3 , Figure 4The screw 173 is threadedly connected to the main body 1 of the culture device, the screw 173 is fixedly connected to the rotating rod 174, and the screw 173 is rotatably connected to the moving plate 172. When the rotating rod 174 is manually rotated, it drives the screw 173 fixedly connected to it to rotate, so that the screw 173 continues to extend into or out of the main body 1 of the culture device, thereby changing the position of the moving plate 172 to ensure flexible positioning of the cell culture plate. The rotatable connection between the screw 173 and the moving plate 172 ensures that the moving plate 172 will not rotate when it is displaced by the rotation of the screw 173.

[0041] See Figure 1 , Figure 2 , Figure 3 , Figure 4 The partition 11 has a U-shaped structure and two sets of vent holes 16, each set containing multiple vent holes 16. The multiple vent holes 16 are evenly and equidistantly distributed. The U-shaped partition 11 and the two sets of vent holes 16 allow the gas entering from the air inlet 18 to first pass through the space between the partition 11 and the main body 1 of the culture device, be heated by the heating plate 5, and then enter the partition 11 through the vent holes 16. When the temperature detected by the sensor 19 does not meet the requirements, the heating plate 5 is used to adjust the gas temperature to a temperature suitable for the survival of mussel blood cells. The evenly and equidistantly distributed vent holes 16 allow the hot gas to enter the partition 11 evenly. The convective heat transfer effect caused by the gas flow process improves the heat transfer efficiency and thermal uniformity.

[0042] See Figure 4 There are two sets of heating plates 5, which are symmetrically arranged. A heat insulation pad 6 is provided on the outside of the heating plate 5. The heat insulation pad 6 is a hollow rectangular structure with one side open. The two sets of symmetrically arranged heating plates 5 can heat the airflow between the partition 11 and the main body of the culture device 1, so that the incoming gas reaches the most suitable temperature for the growth of mussel blood cells. The heat insulation pad 6 can form a protection on the outside of the heating plate 5 to prevent the main body of the culture device 1 from being affected by the heating plate 5.

[0043] Example 2:

[0044] See Figure 1 , Figure 2 , Figure 3 , Figure 4The top of the culture device body 1 has a limiting groove 4, the bottom inner side of the culture device body 1 has a first through groove 2 and a first locking groove 3, the bottom of the top cover 7 has a limiting block 8, the top cover 7 has a second through groove 9 and a second locking groove 10, the top of the partition 11 has a top edge 12 and a top locking strip 13, the bottom of the partition 11 has a bottom edge 14 and a bottom locking strip 15, the bottom edge 14 and the bottom locking strip 15 extend into the first through groove 2 and the first locking groove 3, the top edge 12 and the top locking strip 13 extend into the second through groove 9 and the second locking groove 10, so that the side of the partition 11 near the air inlet 18 forms a relatively sealed space with the culture device body 1 and the top cover 7, so that the auxiliary airflow can participate in the heating as much as possible before entering the inner side of the partition 11, and at the same time, the installation and disassembly process of the top cover 7 and the partition 11 is simpler and faster.

[0045] See Figure 1 , Figure 2 , Figure 3 , Figure 4 The top cover 7 is provided with fixing plates 22 on both sides. The fixing plates 22 are fixedly connected to the top cover 7. The top cover 7 is fixedly connected to the limiting block 8. The setting of the fixing plates 22 makes it convenient for the hands to apply force on both sides of the top cover 7 when the top cover 7 is removed, so that the top cover 7 is separated from the main body 1 of the culture device and the partition 11. When the fixing plates 22 are pulled upward by force, they drive the top cover 7 fixed to them to move upward, thereby causing the limiting block 8 fixed at the bottom of the top cover 7 to move out of the limiting groove 4.

[0046] See Figure 1 , Figure 2 , Figure 3 , Figure 4 There are two sets of limiting blocks 8, with two limiting blocks 8 in each set. The two limiting blocks 8 are symmetrically arranged and engage with the limiting groove 4. The two symmetrical limiting blocks 8 in each set can engage with the limiting groove 4 at multiple positions on the bottom of the top cover 7 to ensure that the four corners of the bottom of the top cover 7 are well fixed and to ensure the engagement and fixation effect between the top cover 7 and the main body 1 of the cultivation device.

[0047] See Figure 1 , Figure 2 , Figure 3 , Figure 4Both the first through groove 2 and the second through groove 9 have a U-shaped structure. The first through groove 2 and the second through groove 9 are consistent with each other. The first through groove 2 and the second through groove 9 are adapted to the top edge 12 and the bottom edge 14. The first locking groove 3 and the second locking groove 10 are consistent with each other. The first locking groove 3 and the second locking groove 10 are engaged with the top locking strip 13 and the bottom locking strip 15. The top edge 12 and the bottom edge 14 can be smoothly inserted into the second through groove 9 and the first through groove 2, respectively. The top locking strip 13 and the bottom locking strip 15 can be smoothly inserted into the second locking groove 10 and the first locking groove 3, respectively. This ensures that the connection between the partition 11 and the bottom of the culture device body 1 and the top cover 7 is tight and without gaps. This allows the gas entering from the air inlet 18 side to be heated and then enter the partition 11 through the vent 16 for the growth of mussel blood cells.

[0048] The implementation principle of this utility model is as follows: First, the main body 1 of the culture device and its interior are thoroughly sterilized and disinfected; second, the bottom edge 14 and bottom locking strip 15 of the partition 11 are aligned with the first through groove 2 and the first locking groove 3 respectively, and the partition 11 is installed; third, the cell culture plate for mussel blood cell culture is placed in the settling tank 170, and according to the size of the cell culture plate used in this experiment, a set of rotating rods 174 are rotated simultaneously to drive the screw 173 to rotate, pushing or pulling a set of moving plates 172 to move, adjusting the position of the set of moving plates 172 until the pad 175 on one side of the set of moving plates 172 abuts against the cell culture plate, and the same steps are used to adjust the position of the other set of moving plates 172 until the two sets of moving plates 172 fix the cell culture plate in the middle of the settling tank 170, above the thin glass slide 21; then, the top cover 7 is limited. Block 8 is aligned with the limiting groove 4. At this time, the second through groove 9 and the second locking groove 10 correspond to the top edge 12 and the top locking strip 13, respectively. The top cover 7 is pressed down so that the limiting block 8 is engaged with the limiting groove 4, the second through groove 9 is engaged with the top edge 12, and the second locking groove 10 is engaged with the top locking strip 13. Then, the sealed culture device body 1 is moved to the microscope stage for fixation so that the center of the thin glass slide 21 is aligned with the light aperture of the objective lens below. Finally, the gas and temperature are monitored using the corresponding sensor 19, and the gas entering from the air inlet 18 is heated by the heating plate 5 so that it enters the inner side of the partition 11 through the vent 16, ensuring that the mussel blood cells on the cell culture plate are cultured in a suitable temperature and gas environment. The stage is moved and the position and focus of the objective lens are adjusted until the cultured cells can be clearly seen in the microscope field of view, so as to perform microscopic observation of the live mussel blood cells.

[0049] All parts not covered in this utility model are the same as or can be implemented using existing technologies, and will not be described in detail here.

[0050] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. A live cell in vitro culture device with a stage, characterized in that: The device includes a culture device body (1), a heating plate (5), a top cover (7), a partition (11), a thin glass slide (21), and a metal sheet (23). An adjustment mechanism (17) is provided at the bottom inside the culture device body (1). The adjustment mechanism (17) includes a settling tank (170), a storage tank (171), and a moving plate (172). A gasket (175) is provided on one side of the moving plate (172), and a screw (173) is provided on the other side of the moving plate (172). A rotating rod (174) is provided on the screw (173). A wire outlet (20) is provided on the front side of the culture device body (1). A sensor (19) is provided on one side of the wire outlet (20). An air inlet (18) is provided on the rear side of the culture device body (1). A vent hole (16) is provided on the partition (11).

2. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The movable plate (172) and the storage slot (171) are each provided in two sets, one set of the movable plate (172) is adapted to one set of storage slots (171), and the other set of the movable plate (172) is adapted to another set of storage slots (171).

3. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The settling trough (170) has a rectangular structure and is connected to the storage trough (171).

4. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The screw (173) is threadedly connected to the main body (1) of the culture device, the screw (173) is fixedly connected to the rotating rod (174), and the screw (173) is rotatably connected to the moving plate (172).

5. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The partition (11) has a U-shaped structure, and the ventilation holes (16) are provided in two sets. Each set of ventilation holes (16) has multiple ventilation holes (16), and the multiple ventilation holes (16) are evenly and equidistantly distributed.

6. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The heating plate (5) is provided in two sets, and the two sets of heating plates (5) are arranged symmetrically. A heat insulation pad (6) is provided on the outside of the heating plate (5), and the heat insulation pad (6) is a hollow rectangular structure with one side open.

7. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The top of the culture device body (1) is provided with a limiting groove (4), the bottom inner side of the culture device body (1) is provided with a first through groove (2) and a first slot (3), the bottom of the top cover (7) is provided with a limiting block (8), the top cover (7) is provided with a second through groove (9) and a second slot (10), the top of the partition (11) is provided with a top edge (12) and a top locking strip (13), and the bottom of the partition (11) is provided with a bottom edge (14) and a bottom locking strip (15).

8. The live cell in vitro culture device on a stage according to claim 1, characterized in that: The top cover (7) is provided with fixing plates (22) on both sides. The fixing plates (22) are fixedly connected to the top cover (7), and the top cover (7) is fixedly connected to the limiting block (8).

9. The live cell in vitro culture device on a stage according to claim 7, characterized in that: The limiting block (8) is provided in two sets, and each set of the limiting block (8) has two blocks. The two limiting blocks (8) are arranged symmetrically, and the limiting block (8) engages with the limiting groove (4).

10. The live cell in vitro culture device on a stage according to claim 7, characterized in that: Both the first through groove (2) and the second through groove (9) are U-shaped structures. The first through groove (2) and the second through groove (9) are consistent. The first through groove (2) and the second through groove (9) are adapted to the top edge (12) and the bottom edge (14). The first slot (3) and the second slot (10) are consistent. The first slot (3) and the second slot (10) are engaged with the top slot strip (13) and the bottom slot strip (15).

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