Perfusion module for tissue staggered perfusion

Abstract

The utility model provides a perfusion module for tissue staggered perfusion, which is provided with at least two sample carrying cavities for carrying biological tissue slice samples, and the sample carrying cavities are communicated through infiltration runners; the sample loading cavity is communicated with a liquid outlet; the infiltration flow channel comprises a flow outlet positioned on the upper side of one end of the sample loading cavity on one side, a flow inlet positioned on the lower side of the other end of the sample loading cavity on the other side, and a communicating channel for communicating the flow outlet with the flow inlet; a height difference exists between the flow outlet and the flow inlet; and the sample loading cavity is communicated with the outside and is provided with a liquid inlet flow channel. By arranging the outflow ports and the inflow ports with the height difference in the sample carrying cavities on the sample carrying plate, a perfusion solution can fully infiltrate biological tissue slice samples in the sample carrying cavities when passing through the perfusion module, and the infiltration effect is ensured.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue section sample perfusion technology, specifically, to a perfusion module for interleaved tissue perfusion. Background Technology

[0002] The perfusion of biological tissue sections under in vitro conditions directly affects the cell viability of the sections during subsequent cryopreservation, depending on factors such as appropriate temperature control, sufficient tissue section infiltration, and stable perfusion solution flow. Existing perfusion devices suffer from challenges such as difficulty in standardizing perfusion conditions, large fluctuations in experimental results, and difficulty in controlling experimental reproducibility and consistency. These factors significantly impact the cell viability of cryopreserved biological tissue sections. Therefore, improving the stability and reproducibility of perfusion has been a pressing issue in this field. Utility Model Content

[0003] This utility model provides an irrigation module for organizing staggered irrigation to overcome the shortcomings of the prior art.

[0004] The perfusion module for staggered perfusion is provided with at least two sample-carrying cavities for carrying biological tissue slice samples, and the sample-carrying cavities are connected by an infiltration channel; the sample-carrying cavities are connected to a drain port; the infiltration channel includes an outlet located on the upper side of one end of one sample-carrying cavity, an inlet located on the lower side of the other end of the other sample-carrying cavity, and a connecting channel connecting the outlet and the inlet; the outlet and the inlet have a height difference; the sample-carrying cavity is connected to an externally provided inlet channel.

[0005] Furthermore, it includes: a sample carrier plate, on which each of the sample carrier cavities and the immersion channel are disposed; a liquid inlet plate, which is pressed onto the sample carrier plate to form a sealed space for each sample carrier cavity; and a liquid inlet channel is provided on the liquid inlet plate to connect the outside world with the sample carrier cavity.

[0006] Furthermore, the multiple sample-carrying cavities are arranged in a group along the horizontal direction on the sample-carrying plate, and the immersion channel is connected between two adjacent sample-carrying cavities in the same group along the front-to-back direction.

[0007] Furthermore, the sample carrier plate is provided with at least two sets of sample carrier cavities arranged in a front-to-back direction; a flow distribution space is provided on the rear side of the sample carrier cavity located at the rear, and each flow inlet on the lower side of the rear end of the sample carrier cavity in each set is connected to the flow distribution space; the liquid inlet channel is connected to the flow distribution space.

[0008] Furthermore, the liquid inlet channel includes a liquid inlet, a liquid inlet outlet, and a liquid inlet channel connecting the liquid inlet and the liquid inlet outlet located on the liquid inlet plate; the diversion space includes a diversion cavity disposed on the sample carrier plate, the extension width of the diversion cavity being set to correspond to the arrangement width of each set of sample carrier cavities, and the diversion cavity being connected to each flow inlet on the lower rear side of each sample carrier cavity; the liquid inlet outlet is disposed corresponding to the diversion cavity.

[0009] Furthermore, the liquid inlet is located on the outer periphery of the liquid inlet plate, and the liquid inlet channel includes a horizontally extending cross channel on the rear side of the liquid inlet plate. A flow channel outlet is provided on the cross channel, and the flow channel outlet is located at the middle position of each group of sample loading chambers. Multiple liquid inlet outlets are evenly distributed and located on the lower side of the liquid inlet plate. Multiple liquid inlet branch channels are evenly distributed and diverted between the flow channel outlet and each liquid inlet outlet.

[0010] Furthermore, the drain port is located on the lower side of the front end of the sample carrier plate, and the drain port is connected to the outlet of the sample carrier cavity located in front.

[0011] Furthermore, it also includes a temperature control plate, which is disposed on the lower side of the sample carrier plate and forms a heat exchange relationship with each of the sample carrier cavities; a drainage slope is provided on the front side of the temperature control plate corresponding to the drain port position, which is inclined downwards towards the front.

[0012] Furthermore, the sample-carrying cavity has an elliptical cross-section, and the outlet and inlet are located at opposite ends of the sample-carrying cavity along its length; the length of each sample-carrying cavity is arranged along the front-back direction of the sample-carrying plate.

[0013] Furthermore, a cylindrical vertically arranged connecting cavity is provided between two adjacent sample-carrying cavities. One end of the connecting cavity extends horizontally to form a first flow channel that communicates with the outlet of one sample-carrying cavity. The other end of the connecting cavity extends horizontally to form a second flow channel that communicates with the inlet of another sample-carrying cavity. The first flow channel, the connecting cavity, and the second flow channel form the connecting channel.

[0014] The beneficial effects of this utility model are as follows:

[0015] The perfusion module can be adapted to combine different sample carrier plates and infusion plates according to the biological tissue section samples and tissue types, meeting the perfusion and wetting requirements of external perfusion solutions for biological tissue section samples. It utilizes a temperature control plate for heat transfer and an infusion plate for liquid injection and diversion, ensuring consistency and repeatability of the perfusion operation.

[0016] The perfusion module features outlets and inlets with varying heights in each sample chamber on the sample carrier plate. This design ensures that the perfusion solution fully infiltrates the biological tissue slices in the sample chambers as it passes through the perfusion module, guaranteeing effective infiltration. Attached Figure Description

[0017] Figure 1 This is an exploded structural diagram of the irrigation module of this utility model;

[0018] Figure 2 yes Figure 1 A magnified view of part A;

[0019] Figure 3 This is a cross-sectional structural diagram of the irrigation module of this utility model;

[0020] Figure 4 yes Figure 3 A magnified view of part B.

[0021] Explanation of reference numerals in the attached figures:

[0022] Sample carrier 1; sample carrier cavity 11, connecting cavity 111, first flow channel 112, second flow channel 113, immersion flow channel 12, outlet 121, connecting channel 122, inlet 123, drain 13.

[0023] Liquid inlet plate 2; liquid inlet channel 21, liquid inlet 22, liquid inlet outlet 23, liquid inlet passage 24, crossflow channel 241, channel outlet 242, liquid inlet branch channel 243, sealing plate 25.

[0024] Flow splitting space 3; Flow splitting cavity 31

[0025] Temperature control plate 4; drainage ramp 41. Detailed Implementation

[0026] To make the technical solution, purpose and advantages of this utility model clearer, the following explanation is given in conjunction with the accompanying drawings and embodiments.

[0027] like Figures 1 to 4 As shown, for the perfusion application of biological tissue slice samples, this utility model provides a perfusion module, which is provided with at least two sample-carrying cavities 11 for carrying biological tissue slice samples, and each sample-carrying cavity 11 is connected to the other via an infiltration channel 12; the sample-carrying cavity 11 is connected to a drain port 13; the infiltration channel 12 includes an outlet 121 located on the upper side of one end of one sample-carrying cavity 11, an inlet 123 located on the lower side of the other end of the other sample-carrying cavity 11, and a connecting channel 122 connecting the outlet 121 and the inlet 123; the outlet 121 and the inlet 123 have a height difference; the sample-carrying cavity 11 is connected to an externally provided liquid inlet channel 21.

[0028] In application, biological tissue slices are placed in the sample-carrying chamber 11 of the perfusion module, and an infusion device is connected to the inlet channel 21 to allow the perfusion solution to be input into the biological tissue slices in the sample-carrying chamber 11. The perfused solution is discharged from the drain port 13, so that it can be used to input the perfusion solution into the sample-carrying chamber 11 for flow perfusion operation. In the perfusion module of this utility model, by designing the wetting channels 12 connecting each sample-carrying chamber 11, including the outlet 121 with a height difference, the inlet 123, and the connecting channel 122, the perfusion solution can fully wet the biological tissue slices in the sample-carrying chamber 11 when passing through the perfusion module, ensuring the wetting effect.

[0029] Example:

[0030] In this embodiment, a preferred structural configuration of the irrigation module is described.

[0031] The perfusion module includes a rectangular plate-shaped sample carrier plate 1 and a liquid inlet plate 2. Each sample carrier cavity 11 and an immersion channel 12 are disposed on the sample carrier plate 1. Each sample carrier cavity 11 is formed by vertical concavity in the sample carrier plate 1, and its cross-section is elliptical with an upper opening. The liquid inlet plate 2 is pressed against the upper side of the sample carrier plate 11 to form a sealed space with each sample carrier cavity 11. The liquid inlet plate 2 is provided with a liquid inlet channel 21 that connects the outside to the sample carrier cavity 11.

[0032] Specifically, taking the length direction of the sample carrier plate 1 as the front-back direction, a plurality of sample carrier cavities 11 are arranged in a group along the horizontal direction on the sample carrier plate 1; the immersion channel 12 is connected between two adjacent sample carrier cavities 11 in the same group along the front-back direction, wherein the outlet 121 and the inlet 123 are respectively located at both ends of the length direction of the sample carrier cavity 11, and a cylindrical vertically arranged connecting cavity 111 is provided between two adjacent sample carrier cavities 11. A first channel 112 is provided on the upper side of one end of the connecting cavity 111 in the horizontal direction and is connected to the outlet 121 in one sample carrier cavity 11; a second channel 113 is provided on the lower side of the other end of the connecting cavity 111 in the horizontal direction and is connected to the inlet 123 in another sample carrier cavity 11; the first channel 112, the connecting cavity 111, and the second channel 113 form the connecting channel 122; at least two groups of a plurality of sample carrier cavities 11 arranged in the front-back direction are provided on the sample carrier plate 1.

[0033] Through the specific arrangement of the immersion channel 12 and the grouped sample loading chambers 11, it can be ensured that during the immersion process, the immersion solution can flow from back to front according to the grouped sample loading chambers 11, thus ensuring the fluidity and flow efficiency of the immersion; at the same time, it ensures that the immersion solution fills each sample loading chamber 11, thus ensuring the immersion effect.

[0034] The sample carrier plate 1 is provided with a diversion space 3 at the rear of the sample carrier cavity 11. Each inlet 123 located at the lower rear end of the sample carrier cavity 11 in each group is connected to the diversion space 3. The liquid inlet channel 21 is connected to the diversion space 3.

[0035] The externally input perfusion solution is introduced into the diversion space 3 through the inlet channel 21 and fills the space. Since the inlet 123 of the diversion space 3 and the rear side of each sample loading chamber 11 are set at the same horizontal position, the perfusion solution will be continuously delivered to each group of sample loading chambers 11 at the same pressure. This satisfies the perfusion and infiltration requirements of biological tissue slice samples in multiple groups of sample loading chambers 11 with a single input, and effectively ensures that the perfusion effect between each group of sample loading chambers 11 is equal.

[0036] Specifically, the liquid inlet channel 21 includes a liquid inlet 22 disposed on the outer periphery of the liquid inlet plate 2, a plurality of liquid inlet outlets 23 evenly distributed and disposed on the lower side of the liquid inlet plate 2, and a liquid inlet channel 24 connecting the liquid inlet 22 and the liquid inlet outlets 23; the liquid inlet channel 24 includes a cross channel 241 extending horizontally on the rear side of the liquid inlet plate 2, a channel outlet 242 disposed on the cross channel 241, the channel outlet 242 being disposed at the middle position of each group of sample-carrying cavities 11, and a plurality of liquid inlet branch channels 243 evenly distributed and diverted between the channel outlet 242 and each liquid inlet outlet 23. The diversion space 3 includes a diversion cavity 31 disposed on the sample carrier plate 1. The extension width of the diversion cavity 31 is set to correspond to the arrangement width of each group of sample carrier cavities 11. The diversion cavity 31 is connected to each flow inlet 123 on the lower rear end of each sample carrier cavity 11. Each liquid outlet 23 is evenly distributed side by side along the width direction and is set to correspond to the position of the diversion cavity 31.

[0037] For ease of processing, the flow channel outlet 242 and the liquid inlet branch channel 243 are machined and configured corresponding to the upper side of the liquid inlet plate 2. In this embodiment, a sealing plate 25 is provided on the rear part of the upper side of the liquid inlet plate 2 to press against the positions of the flow channel outlet 242 and the liquid inlet branch channel 243, so as to form a flow channel seal.

[0038] The drain port 13 is located on the lower front side of the sample carrier plate 1, and is connected to the outlet 121 of the sample carrier cavity 11 located in front. Specifically, the drain port 13 has the same structure as the inlet 123, allowing the outlet 121 of the sample carrier cavity 11 to flow out through the connecting channel 122 to the drain port 13. In order to meet the temperature control requirements of the perfusion module during the perfusion process, the perfusion module also includes a temperature control plate 4, which is located on the lower side of the sample carrier plate 1 and forms a heat exchange relationship with each of the sample carrier cavities 11. The front side of the temperature control plate 4 is provided with a downwardly inclined drain ramp 41 corresponding to the drain port 13. The drain ramp 41 facilitates the discharge of the tissue perfusion solution.

[0039] The above description is only a preferred embodiment of the present utility model. For those skilled in the art, modifications can still be made to the embodiments without departing from the implementation principle of the present utility model, and the corresponding modifications should also be considered within the protection scope of the present utility model.

Claims

1. An irrigation module for organizing staggered irrigation, characterized in that, The sample carrier chamber is provided with at least two sample-carrying cavities for holding biological tissue slices, and the sample-carrying cavities are connected by an infiltration channel; the sample-carrying cavities are connected to a drain port; the infiltration channel includes an outlet located on the upper side of one end of one sample-carrying cavity, an inlet located on the lower side of the other end of the other sample-carrying cavity, and a connecting channel connecting the outlet and the inlet; the outlet and the inlet have a height difference; the sample-carrying cavity is connected to an externally provided inlet channel.

2. The irrigation module as described in claim 1, characterized in that, include: A sample carrier plate, wherein each of the aforementioned sample carrier cavities and immersion channels is disposed on the sample carrier plate; The liquid inlet plate is pressed onto the sample carrier plate to form a sealed space for each sample carrier cavity; the liquid inlet plate is provided with a liquid inlet channel that connects the outside world to the sample carrier cavity.

3. The irrigation module as described in claim 2, characterized in that, Multiple sample-carrying cavities are arranged in a group along the horizontal direction on the sample-carrying plate, and the immersion channel is connected between two adjacent sample-carrying cavities in the same group along the front-to-back direction.

4. The irrigation module as described in claim 3, characterized in that, The sample carrier plate is provided with at least two sets of sample carrier cavities arranged in a front-to-back direction; a flow distribution space is provided on the rear side of the sample carrier cavity located at the rear, and each flow inlet on the lower rear end of the sample carrier cavity in each set is connected to the flow distribution space; the liquid inlet channel is connected to the flow distribution space.

5. The irrigation module as described in claim 4, characterized in that, The liquid inlet channel includes a liquid inlet, a liquid outlet, and a liquid inlet passage connecting the liquid inlet and the liquid outlet on the liquid inlet plate; the diversion space includes a diversion cavity on the sample carrier plate, the extension width of the diversion cavity is set to correspond to the arrangement width of each set of sample carrier cavities, and the diversion cavity is connected to each flow inlet on the lower rear side of each sample carrier cavity; the liquid outlet is set to correspond to the diversion cavity.

6. The irrigation module as described in claim 5, characterized in that, The liquid inlet is located on the outer periphery of the liquid inlet plate. The liquid inlet channel includes a horizontally extending cross channel on the rear side of the liquid inlet plate. A flow channel outlet is provided on the cross channel. The flow channel outlet is located at the middle position of each group of sample loading chambers. Multiple liquid inlet outlets are evenly distributed and located on the lower side of the liquid inlet plate. Multiple liquid inlet branch channels are evenly distributed and diverted between the flow channel outlet and each liquid inlet outlet.

7. The irrigation module as described in claim 2, characterized in that, The drain port is located on the lower front side of the sample carrier plate, and the drain port is connected to the outlet of the sample carrier cavity located in front.

8. The irrigation module as described in claim 7, characterized in that, It also includes a temperature control plate, which is disposed on the lower side of the sample carrier plate and forms a heat exchange relationship with each of the sample carrier cavities; the front side of the temperature control plate is provided with a drainage slope that is inclined downwards towards the front side corresponding to the position of the drainage port.

9. The irrigation module as described in any one of claims 1 to 8, characterized in that, The sample-carrying cavity has an elliptical cross-section, and the outlet and inlet are located at opposite ends of the length of the sample-carrying cavity.

10. The irrigation module as described in claim 9, characterized in that, A cylindrical vertical connecting cavity is provided between two adjacent sample-carrying cavities. One end of the connecting cavity extends horizontally to form a first flow channel that communicates with the outlet of one sample-carrying cavity. The other end of the connecting cavity extends horizontally to form a second flow channel that communicates with the inlet of another sample-carrying cavity. The first flow channel, the connecting cavity, and the second flow channel form the connecting channel.

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