Centrifugal tube for preparing platelet-rich plasma with low cell breakage rate
By designing the inlet tube to be angled and the filter paper structure to be breathable in the centrifuge tube, the problem of high cell damage rate was solved, and the preparation of highly active platelet-rich plasma was achieved, thus improving the clinical treatment effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- 大庆市萨尔图区郭燕庆健康咨询工作室
- Filing Date
- 2025-04-17
- Publication Date
- 2026-05-19
AI Technical Summary
The current platelet-rich plasma preparation process suffers from a high rate of cell damage, which affects cell viability and clinical application efficacy.
A centrifuge tube for preparing platelet-rich plasma with low cell damage rate was designed. The tube is set at an angle and combined with an inner wall drainage and air-permeable filter paper structure to reduce damage caused by cell impact and vibration with the tube wall.
It effectively reduces cell damage rate, improves cell integrity and activity in platelet-rich plasma, and enhances clinical application efficacy.
Smart Images
Figure CN224252853U_ABST
Abstract
Description
Technical Field
[0001] This application relates to a centrifuge tube for preparing platelet-rich plasma (PRP) with low cell damage rate. Background Technology
[0002] Platelet-rich plasma (PRP) is generally prepared by centrifugation. The most important component in this process is the centrifuge tube. There are generally two strategies for transferring whole blood into the centrifuge tube:
[0003] One method involves pre-installing a conduit inside the centrifuge tube. This conduit, while serving to guide blood into the centrifuge tube, prevents cell damage during transfer. However, during centrifugation, fragile blood cells can be damaged due to the following reasons: 1. The conduit itself, located within the centrifuge tube, transmits vibrations during centrifugation, causing it to vibrate; 2. Resonance or synchrotron vibrations occur during centrifugation, causing conduit vibration; 3. The conduit itself vibrates at high frequency during centrifugation. These three factors combined create a "stirring rod" effect, similar to a pulverizer, resulting in the destruction of a large number of fragile blood cells. 4. Cells do not travel vertically; the conduit in the centrifuge tube adds a cell impact interface, leading to cell damage. After leaving the vascular endothelial environment, blood cells are extremely fragile, and the combined effect of the above factors results in a very high cell damage rate, thus affecting the clinical efficacy of viable cells.
[0004] Another method involves not installing internal tubing and instead directly introducing whole blood through an opening in the cap. While this method avoids the "crusher effect" of the stirring rod during centrifugation and reduces cell damage during the process, the high speed and transfer height of the injection tube during cell introduction lead to greater impact speed between the cells and the tube wall, which can also increase cell damage. Damaged cells can then be further destroyed during centrifugation, affecting the integrity of platelet-rich plasma preparation.
[0005] Therefore, improvements are needed for the two structural forms mentioned above to minimize or avoid the problems as much as possible, thereby reducing the damage rate of blood cells during centrifugation, improving the activity of the prepared PRP, and enhancing clinical efficacy. Utility Model Content
[0006] To address the aforementioned issues, this application proposes a centrifuge tube for preparing platelet-rich plasma with low cell damage rate. The tube includes a tube body, an upper cap, an input channel on the upper cap, and an inlet tube at the end of the input channel facing the tube body. The inlet tube is angled towards the inner wall of the tube body. Several delivery channels are also provided on the upper cap. This application utilizes the angled inlet tube design, which allows for drainage through the inner wall of the tube body, providing excellent buffering and preventing cell damage during transfer. Because the inlet tube is short, it does not come into contact with blood or blood cells during centrifugation, thus preventing duct-related damage and ensuring the integrity of the cells obtained from platelet-rich plasma preparation. This improves cell viability and enhances clinical efficacy. The angled inlet tube designation refers to the inlet tube being closer to the inner wall of the tube body than the outlet, and it is not limited to whether the inlet tube is partially or entirely angled.
[0007] Preferably, the inlet tube includes a vertical tube connected to the input channel, and a bent tube is provided at the end of the vertical tube away from the upper tube cover, which bends toward the inner wall of the tube body. The angle of the outlet of the bent tube is α, where 5°≤α≤170°.
[0008] Preferably, the angle of the bend outlet is 90°, and an external outlet tube is also provided on the side of the bend away from the vertical tube. The inlet tube of this application is set at an upward angle, which can better utilize the inner wall of the tube to receive and slow down the introduced blood, thereby further ensuring that damage to the internal cells is avoided due to direct impact between blood cells and the tube wall during blood introduction.
[0009] Preferably, the bending angle of the end of the bend is set to be diagonally upward, horizontal, or diagonally downward.
[0010] Preferably, the distance between the outermost end of the inlet tube near the tube body and the inner wall of the inclined tube body is 0.5-20mm.
[0011] Preferably, the delivery channel is provided with breathable filter paper.
[0012] Preferably, the upper tube cap is provided with several connecting tubes, and an auxiliary cap is sleeved on the connecting tube. The auxiliary cap communicates with the connecting tube in the middle to form a delivery channel. The breathable filter paper is located in the middle of the auxiliary cap, and an auxiliary protective cap is provided at the top of the delivery channel. This application, by setting the auxiliary cap and the internal breathable filter paper, allows for the smooth introduction of internal gas during introduction, and when export is required, export can be completed by piercing the breathable filter paper with a syringe. This results in better operational stability and greater convenience.
[0013] Preferably, the inlet tube extends through the upper tube cover, an inlet cap is fitted onto the top of the inlet tube, the middle part of the inlet cap is connected to the inlet tube, and an inlet protective cap is fitted onto the top of the inlet cap.
[0014] Preferably, the number of the pick-up and delivery channels is two.
[0015] Preferably, the upper tube cap is threaded to the inner or outer side of the tube body, or the upper tube cap is sleeved to the inner or outer side of the tube body.
[0016] This application can bring the following beneficial effects:
[0017] 1. This application adopts an oblique tube setting, which can utilize the inner wall of the tube for drainage, and has a very good buffering effect, avoiding damage to cells during transfer. Because the inlet tube is short, it will not cause damage to catheter-related cells during centrifugation, thus ensuring the integrity of cells in the obtained platelet-rich plasma, improving PRP activity, and improving clinical efficacy.
[0018] 2. The infusion tube of this application is preferably set at an upward angle, which can better utilize the inner wall of the tube to receive and slow down the infused blood, thereby further ensuring that damage to the internal cells is avoided during blood infusion.
[0019] 3. By setting an auxiliary cap and an internal breathable filter paper, this application can smoothly introduce the internal gas during introduction, and when it is necessary to export, it can be done by piercing the breathable filter paper with a syringe. The operation is more stable and more convenient. Attached Figure Description
[0020] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings:
[0021] Figure 1 This is a schematic diagram of the structure of this application.
[0022] Figure 2 This is a schematic diagram of a structure with an external discharge tube. Detailed Implementation
[0023] To clearly illustrate the technical features of this solution, the following detailed description, in conjunction with the accompanying drawings, will be provided.
[0024] In the first embodiment, such as Figure 1As shown, a centrifuge tube for preparing platelet-rich plasma with low cell damage rate includes a tube body 1, an upper tube cap 2 on the tube body 1, an input channel 3 on the upper tube cap 2, and an inlet tube 4 at one end of the input channel 3 facing the tube body 1. The inlet tube 4 is inclined towards the inner wall of the tube body 1. Several delivery channels 5 are also provided on the upper tube cap 2.
[0025] In operation, the blood to be separated is first introduced into tube 1 through inlet channel 3 and inlet tube 4. The entire tube is then placed in a centrifuge for centrifugation. After the first centrifugation, a long needle is inserted through pick-and-place channel 5 via a syringe. The syringe aspirates the red blood cells from the bottom layer. The syringe and the aspirated red blood cells are then removed, and the tube is placed back into the centrifuge for a second centrifugation. After the second centrifugation, a suitable amount of platelet-rich plasma is extracted from the bottom of tube 1 through pick-and-place channel 5 via a syringe, thus completing the separation process.
[0026] In the second embodiment, as Figure 1-2 As shown, a centrifuge tube for preparing platelet-rich plasma with low cell damage rate includes a tube body 1, an upper tube cap 2 on the tube body 1, an input channel 3 on the upper tube cap 2, and an inlet tube 4 at one end of the input channel 3 facing the tube body 1. The inlet tube 4 is inclined towards the inner wall of the tube body 1. Several delivery channels 5 are also provided on the upper tube cap 2.
[0027] The inlet tube 4 includes a vertical tube 6 connected to the inlet channel 3. At the end of the vertical tube 6 furthest from the upper cap 2, a bent tube 7 is provided, bending towards the inner wall of the tube body 1. The outlet angle of the bent tube 7 is α, where 5°≤α≤170°. The outlet angle of the bent tube 7 is 90°. An external outlet tube 8 is also provided on the side of the bent tube 7 furthest from the vertical tube 6. The bending angle of the end of the bent tube 7 can be angled upwards, horizontally, or downwards. An upward angle minimizes damage to blood cells caused by impact with the tube wall during inlet insertion.
[0028] The distance between the outermost end of the inlet tube 4 near the end of the tube body 1 and the inner wall of the inclined tube body 1 is 0.5-20mm. The inner wall of the tube body 1 is used for guidance.
[0029] A breathable filter paper 9 is provided on the delivery channel 5. Several connecting tubes 10 are provided on the upper tube cover 2. An auxiliary cap 11 is fitted onto each connecting tube 10, and the auxiliary cap 11 communicates with the connecting tube 10 in the middle to form the delivery channel 5. The breathable filter paper 9 is located in the middle of the auxiliary cap 11. An auxiliary protective cap 12 is provided at the top of the delivery channel 5. The inlet tube 4 extends out of the upper tube cover 2, and an inlet cap 13 is fitted onto the top of the inlet tube 4. The middle of the inlet cap 13 communicates with the inlet tube 4, and an inlet protective cap 14 is fitted at the top of the inlet cap 13. There are two delivery channels 5.
[0030] The upper tube cap 2 is threadedly connected to the inner or outer side of the tube body 1, or the upper tube cap 2 is sleeved to the inner or outer side of the tube body 1.
[0031] In operation, the blood to be separated is first introduced into the tube body 1 through the inlet channel 3 and the inlet tube 4. The entire tube is then placed in a centrifuge for centrifugation. After the first centrifugation, the auxiliary protective cap 12 on the first delivery channel 5 is opened. A syringe with a long needle is then inserted through the delivery channel 5, passing through the auxiliary cap 11 and the breathable filter paper 9 in sequence. The syringe aspirates the red blood cells from the bottom layer. The syringe and the aspirated red blood cells are then removed, and the tube is placed back into the centrifuge for a second centrifugation. After the second centrifugation, the auxiliary protective cap 12 on the second delivery channel 5 is opened. A syringe with a long needle is then inserted through the delivery channel 5, passing through the auxiliary cap 11 and the breathable filter paper 9 in sequence, to extract an appropriate amount of platelet-rich plasma from the bottom of the tube body 1, thus completing the separation process.
[0032] The above are merely embodiments of this application and are not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.
Claims
1. A centrifuge tube for preparing platelet-rich plasma with a low cell breakage rate, characterized by: It includes a tube body, an upper tube cover on the tube body, an input channel on the upper tube cover, and an inlet tube at one end of the input channel facing the tube body, the inlet tube being inclined toward the inner wall of the tube body; the upper tube cover also has several delivery channels.
2. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 1, characterized in that: The inlet tube includes a vertical tube connected to the input channel, and a bent tube is provided at the end of the vertical tube away from the upper tube cover, which bends toward the inner wall of the tube body. The angle of the outlet of the bent tube is α, where 5°≤α≤170°.
3. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 2, characterized in that: The angle of the bend outlet is 90°, and an external outlet pipe is also provided on the side of the bend away from the vertical pipe.
4. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 2, characterized in that: The bending angle of the end of the bend is set to be diagonally upward, horizontal, or diagonally downward.
5. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 1, characterized in that: The distance between the outermost end of the inlet tube near the tube body and the inner wall of the inclined tube body is 0.2-20mm.
6. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 1, wherein: The pick-and-place channel is equipped with breathable filter paper.
7. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 1, wherein: The upper tube cover is provided with several connecting tubes, and an auxiliary cap is fitted onto the connecting tube. The middle part of the auxiliary cap is connected to the connecting tube to form a feeding channel. The air-permeable filter paper is placed in the middle of the auxiliary cap, and an auxiliary protective cover is provided at the top of the feeding channel.
8. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 1, wherein: The inlet tube extends through the upper tube cover, and an inlet cap is fitted onto the top of the inlet tube. The middle part of the inlet cap is connected to the inlet tube, and an inlet protective cap is installed on the top of the inlet cap.
9. A centrifuge tube for preparing platelet-rich plasma with low cell damage rate according to claim 1, characterized in that: The number of pick-up and delivery channels is two.
10. The low cell disruption rate platelet rich plasma preparation centrifuge tube according to claim 1, wherein: The upper tube cap is threaded to the inner or outer side of the tube body, or the upper tube cap is sleeved to the inner or outer side of the tube body.