Water body nucleic acid enrichment instrument

Abstract

The utility model provides a water body nucleic acid enrichment instrument, which relates to the technical field of water body nucleic acid detection and comprises a box body, a filter bin, an enrichment bin and an equipment bin are distributed in the box body, a filter bin cover is arranged on the filter bin, a first feeding hole is formed in the filter bin cover, and the upper end of the first feeding hole is sequentially connected with a valve, a filter and a feeding increment pipe. The top of the enrichment bin is an enrichment bin top cover, a second feeding hole is formed in the enrichment bin top cover, the upper end of the second feeding hole is sequentially connected with a valve, an enrichment adsorption device and a feeding increment pipe, and a negative pressure device is arranged in the equipment bin. The device can effectively reduce the difficulty of water body enrichment operation before water body nucleic acid detection, has the characteristics of simplicity in use, convenience in operation and convenience in carrying, and has practicability.

Description

Technical Field

[0001] This utility model relates to the field of water body nucleic acid detection technology, and in particular to a water body nucleic acid enrichment instrument. Background Technology

[0002] Nucleic acids in water mainly consist of DNA and RNA. As the basic building blocks of genetic material in organisms, nucleic acids are a class of biopolymers, widely distributed in all known life forms, and are one of the most crucial biomolecules. Nucleic acids, namely deoxyribonucleic acid and ribonucleic acid, are polymers of numerous nucleotide monomers, belonging to biological macromolecules and are one of the most fundamental substances for maintaining life activities.

[0003] Aquatic nucleic acid testing refers to the detection and analysis of nucleic acids in target organisms within water bodies using specific methods and technologies. This testing method is of paramount importance in safeguarding public health and preventing the spread of diseases.

[0004] Currently, before conducting nucleic acid testing in water bodies, it is essential to enrich the nucleic acids in the water. However, conventional enrichment methods are complex, and the equipment used is mostly independently configured, which undoubtedly increases the operational difficulty and necessitates a more convenient and efficient solution. Summary of the Invention

[0005] In response to the problems mentioned in the background art, this utility model provides a water nucleic acid enrichment instrument.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A water-based nucleic acid enrichment instrument includes a housing with an openable cover installed at the top. The housing contains a filtration chamber, an enrichment chamber, and an equipment chamber.

[0008] The filter chamber is provided with an openable filter chamber cover. The filter chamber cover is provided with several first inlet holes that communicate with the inside of the filter chamber. The upper end of the first inlet hole is connected to a valve, a filter and an inlet increment pipe in sequence. A collection bottle is provided inside the filter chamber.

[0009] The top of the enrichment chamber is an enrichment chamber top cover, and the enrichment chamber top cover is provided with several second inlet holes that communicate with the interior of the enrichment chamber. The upper end of the second inlet hole is connected in sequence to a valve, an enrichment adsorption device and an inlet increment pipe.

[0010] The equipment compartment has a built-in negative pressure device, which corresponds one-to-one with the filtration compartment and the enrichment compartment, and is connected to each other.

[0011] Furthermore, the filter is a needle filter, the enrichment adsorption device is an enrichment column, and the negative pressure device is a negative pressure generator;

[0012] Furthermore, a discharge pipe communicating with the enrichment bin is provided on the side of the box body, and a discharge valve is provided on the discharge pipe. The bottom of the enrichment bin is a sloping slope that extends towards the discharge pipe and gradually decreases in height.

[0013] Furthermore, the filter chamber and the filter chamber cover are connected by a hinge and can be opened and closed, and the opening of the filter chamber and the filter chamber cover are sealed by a slot and a sealing ring.

[0014] Furthermore, the front of the housing is provided with one-way pressure relief valves that are respectively connected to the filter chamber and the enrichment chamber;

[0015] Furthermore, a control panel is integrated on the side of the enclosure;

[0016] Furthermore, the box lid is provided with a storage structure, which includes a bag body. The top of the bag body is fitted with a seal, and the bottom of the seal is fitted with a snap.

[0017] The technical effects and advantages of this utility model are as follows:

[0018] This utility model proposes a water nucleic acid enrichment instrument, relating to the field of water nucleic acid detection technology. It includes a housing containing a filtration chamber, an enrichment chamber, and an equipment chamber. The filtration chamber has a cover with a first inlet hole, the upper end of which is connected to a valve, a filter, and an inlet increment pipe. The enrichment chamber has a top cover with a second inlet hole, the upper end of which is connected to a valve, an enrichment adsorption device, and an inlet increment pipe. The equipment chamber has a built-in negative pressure device. This utility model has a reasonable design and simple principle, integrating water filtration and enrichment equipment, effectively reducing the difficulty of water enrichment operations before water nucleic acid detection. It is also simple to use, easy to operate, and portable, making it practical. Attached Figure Description

[0019] Figure 1 This is a perspective view of the present utility model.

[0020] Figure 2 This is a cross-sectional view of the present invention.

[0021] The attached diagrams are labeled as follows: 1. Box body; 2. Box cover; 3. Filter chamber; 4. Enrichment chamber; 5. Equipment chamber; 6. Filter chamber cover; 7. First inlet port; 8. Valve; 9. Filter; 10. Incremental feed pipe; 11. Collection bottle; 12. Enrichment chamber top cover; 13. Second inlet port; 14. Enrichment adsorption device; 15. Negative pressure device; 16. Discharge pipe; 17. Discharge valve; 18. Sloping surface; 19. Slot; 20. Sealing ring; 21. One-way pressure relief valve; 22. Control panel; 23. Storage structure; 24. Bag body; 25. Sealing; 26. Snap fastener. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] As shown in the figure, this embodiment proposes a water nucleic acid enrichment instrument for filtering and enriching water nucleic acid before detection. The water nucleic acid enrichment instrument includes a box 1, and the top of the box 1 is equipped with an openable box cover 2, which is convenient to use. In addition, the box 1 contains a filter chamber 3, an enrichment chamber 4 and an equipment chamber 5 with different functions.

[0024] The filter chamber 3 is used for water filtration. It is equipped with an openable filter chamber cover 6. The filter chamber cover 6 is provided with several first feed holes 7 that communicate with the inside of the filter chamber 3. The upper end of the first feed holes 7 is connected in sequence to a detachable valve 8, a filter 9 and a feed increment pipe 10. In use, the valve 8, the filter 9 and the feed increment pipe 10 are connected in sequence. In addition, the filter chamber 3 is also equipped with a collection bottle 11 for collecting the filtered water.

[0025] Among them, the enrichment chamber 4 is used for the enrichment of nucleic acids in water. Its top is the enrichment chamber top cover 12 (normally closed). The enrichment chamber top cover 12 is provided with several second feed holes 13 that communicate with the inside of the enrichment chamber 4. The upper end of the second feed hole 13 is connected in sequence to the detachable valve 8, the enrichment adsorption device 14 and the feed increment pipe 10. When in use, the valve 8, the enrichment adsorption device 14 and the feed increment pipe 10 are connected in sequence.

[0026] The equipment compartment 5 is used to place the equipment. It has a built-in negative pressure device 15, which corresponds to the filter compartment 3 and the enrichment compartment 4 and is connected to them respectively. It is used to create negative pressure inside the filter compartment 3 and the enrichment compartment 4 and adjust their vacuum degree.

[0027] The working method of this water-based nucleic acid enrichment instrument is as follows:

[0028] First, water filtration is performed. The collection bottle 11 is placed into the filter chamber 3, and then the filter chamber cover 6 is closed. The valve 8, filter 9, and feed increment pipe 10 are installed sequentially at the first feed inlet 7. After installation, the water sample is poured into the feed increment pipe 10. Then, the negative pressure device 15 is activated to create a negative pressure inside the filter chamber 3. Under atmospheric pressure, the water sample in the feed increment pipe 10 is filtered by the filter 9 and finally falls into the collection bottle 11 in the filter chamber 3, completing the filtration and collection. During this process, the following points should be noted: the unused first feed inlet 7 on the filter chamber cover 6 needs to be sealed. The sealing method can be to insert the valve 8 in the closed state. In addition, the vacuum degree inside the filter chamber 3 needs to be controlled to prevent agitation and splashing.

[0029] Next comes the enrichment of nucleic acids in the water (before enrichment, the filtered water needs to undergo additional treatment, such as cell lysis and nucleic acid precipitation). A valve 8, an enrichment adsorption device 14, and an increment tube 10 are installed at the second inlet port 13. After installation, the water to be enriched is poured into the increment tube 10. Then, the negative pressure device 15 is activated to create a negative pressure inside the enrichment chamber 4. Under atmospheric pressure, the water to be enriched in the increment tube 10 is enriched by the enrichment adsorption device 14. The remaining water is discarded and eventually falls into the enrichment chamber 4, while the target nucleic acid remains on the enrichment adsorption device 14. The enrichment adsorption device 14 can then be removed for subsequent extraction and detection of nucleic acids in the water. During this process, the unused second inlet port 13 also needs to be sealed.

[0030] To facilitate nucleic acid testing in water and meet relevant standards, the filter 9 in this embodiment is a needle filter, the enrichment adsorption device 14 is an enrichment column, and the negative pressure device 15 is a negative pressure generator.

[0031] To facilitate wastewater discharge, this embodiment provides a discharge pipe 16 connected to the enrichment chamber 4 on the side of the housing 1. The discharge pipe 16 is equipped with a discharge valve 17 for controlling drainage. In addition, to prevent wastewater residue, the bottom of the enrichment chamber 4 in this embodiment is designed as a sloping slope 18, which extends towards the discharge pipe 16 and gradually decreases in height.

[0032] To improve ease of use, the filter chamber 3 and the filter chamber cover 6 are connected by a hinge and can be opened and closed. At the same time, to ensure vacuum, the opening of the filter chamber 3 and the filter chamber cover 6 are sealed by a slot 19 and a sealing ring 20. In addition, the enrichment chamber top cover 12 and the enrichment chamber 4 are normally closed, but can also be opened and closed to facilitate regular cleaning of the inside of the enrichment chamber 4.

[0033] In addition, the front of the housing 1 is provided with one-way pressure relief valves 21 that are connected to the filter chamber 3 and the enrichment chamber 4 respectively, for quickly depressurizing the filter chamber 3 and the enrichment chamber 4 (in actual use, when the negative pressure device 15 is closed and the feed increment pipe 10 is emptied, it can depressurize on its own). In order to facilitate equipment control, this embodiment integrates a control panel 22 on the side of the housing 1 for controlling the operation of the equipment.

[0034] Meanwhile, in this embodiment, a storage structure 23 is provided inside the box cover 2. The storage structure 23 includes a bag body 24, a seal 25 is installed at the top of the bag body 24, and a snap 26 is installed at the bottom of the seal 25 for storing work tools, such as valves 8, filters 9, enrichment adsorption devices 14, and feed increment pipes 10.

[0035] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," "join," and "fix" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0036] All standard parts used in this invention can be purchased from the market, and irregular parts can be customized according to the description and drawings.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A water-based nucleic acid enrichment instrument, comprising a housing (1), wherein the top of the housing (1) is fitted with an openable cover (2), characterized in that, The housing (1) contains a filter chamber (3), an enrichment chamber (4), and an equipment chamber (5); The filter chamber (3) is provided with an openable filter chamber cover (6), and the filter chamber cover (6) is provided with several first feed holes (7) that communicate with the inside of the filter chamber (3). The upper end of the first feed hole (7) is connected to a valve (8), a filter (9) and a feed increment pipe (10) in sequence. A collection bottle (11) is provided inside the filter chamber (3). The top of the enrichment chamber (4) is an enrichment chamber top cover (12). The enrichment chamber top cover (12) is provided with several second feed holes (13) that communicate with the interior of the enrichment chamber (4). The upper end of the second feed holes (13) is connected to a valve (8), an enrichment adsorption device (14), and a feed increment pipe (10) in sequence. The equipment compartment (5) has a built-in negative pressure device (15), which corresponds one-to-one with the filter compartment (3) and the enrichment compartment (4) and is connected to each other.

2. The water nucleic acid enrichment instrument according to claim 1, characterized in that, The filter (9) is a needle filter, the enrichment adsorption device (14) is an enrichment column, and the negative pressure device (15) is a negative pressure generator.

3. The water nucleic acid enrichment instrument according to claim 1, characterized in that, The side of the box (1) is provided with a discharge pipe (16) that communicates with the enrichment bin (4). The discharge pipe (16) is equipped with a discharge valve (17). The bottom of the enrichment bin (4) is a sloping slope (18). The sloping slope (18) extends toward the discharge pipe (16) and gradually decreases in height.

4. The water nucleic acid enrichment instrument according to claim 3, characterized in that, The filter chamber (3) and the filter chamber cover (6) are connected by a hinge and can be opened and closed. The opening of the filter chamber (3) and the filter chamber cover (6) are sealed by a slot (19) and a sealing ring (20).

5. A water-based nucleic acid enrichment instrument according to any one of claims 1, 3, and 4, characterized in that, The front of the housing (1) is provided with a one-way pressure relief valve (21) that is connected to the filter chamber (3) and the enrichment chamber (4) respectively.

6. The water nucleic acid enrichment instrument according to claim 1, characterized in that, The control panel (22) is integrated on the side of the housing (1).

7. The water nucleic acid enrichment instrument according to claim 1, characterized in that, The lid (2) is provided with a storage structure (23), which includes a bag (24). The top of the bag (24) is fitted with a seal (25), and the bottom of the seal (25) is fitted with a snap (26).

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