NK cell preparation and culture device

Abstract

The utility model discloses an NK (Natural Killer) cell preparation and culture device, which relates to the technical field of biological cell culture equipment and comprises a bottom shell, a culture structure, a sealing structure and an extraction structure. The culture structure is provided with a plurality of culture grooves, the sealing structure is nested with the sealing groove through the sealing cover to form a sterile environment, and the magnetic permeable shell allows a magnetic field to penetrate so as to realize non-contact magnetic attraction positioning. The extraction structure is attracted and positioned through a first magnetic attraction block and a second magnetic attraction block, an electric telescopic rod drives an extraction pipe to vertically ascend and descend, a negative pressure system communicates with a sealing cover through a butt joint, and sealed negative pressure extraction is achieved. The device supports accurate extraction of cell sap in any culture tank through magnetic attraction guiding and closed flow channel design, isolates external pollution in the whole process, and is suitable for large-scale culture of NK cells with high cleanliness requirements.

Description

Technical Field

[0001] This utility model relates to the field of biological cell culture equipment technology, specifically: an NK cell preparation and culture device. Background Technology

[0002] NK cells (natural killer cells) play a crucial role in immunotherapy, and their in vitro culture requires a strictly aseptic environment. Traditional culture devices often use open culture dishes or multi-well plates, necessitating frequent opening of the sealed caps for sampling, which poses a risk of microbial contamination. While some closed culture systems can reduce contamination, the sampling process relies on manual operation, which is inefficient and can easily disrupt the culture environment, affecting cell viability. For example: High risk of contamination: Traditional devices require exposure of the culture environment during sampling, easily introducing external microorganisms. Insufficient operational precision: Manual sampling makes it difficult to accurately control the sampling location and depth, potentially leading to cell damage or cross-contamination of samples. Utility Model Content

[0003] In view of the problems in the related technologies, this utility model proposes an NK cell preparation and culture device to overcome the above-mentioned technical problems existing in the existing related technologies.

[0004] Therefore, the specific technical solution adopted by this utility model is as follows:

[0005] An NK cell preparation and culture device includes a bottom shell, a culture structure placed on the bottom shell, a sealing structure covering the culture structure, and an extraction structure connected to the sealing structure. The culture structure achieves a relatively sealed, pollution-proof culture function through the sealing structure, and achieves a sealed extraction function through the extraction structure.

[0006] Furthermore, the culture structure includes a culture plate, a culture tank, a sealing groove, and connecting ears. The culture plate has culture tanks evenly distributed inside, a sealing groove is opened on the inner side of the top of the culture plate, and connecting ears are fixedly connected around the perimeter of the culture plate. The culture plate fits into the bottom shell.

[0007] Furthermore, the sealing structure includes a sealing cover, a magnetic shell, and a connecting pipe. The magnetic shell is fixedly mounted on the sealing cover, and the connecting pipe is provided on one side of the sealing cover. The sealing cover is sealed and placed on the sealing groove.

[0008] Furthermore, the extraction structure includes a first magnetic block, a gripping block, a push switch, a guide block, a mounting block, a second magnetic block, an electric telescopic rod, an extraction tube, a connecting tube, a solenoid valve, a telescopic joint, a negative pressure tube, a connecting seat, and a connector. A gripping block is fixedly connected to the first magnetic block, and a push switch is mounted on the gripping block. Guide blocks are fixedly connected around the first magnetic block, and a caster wheel is fixedly mounted at the bottom of the guide blocks, contacting the top of the magnetic shell. A second magnetic block is mounted on the bottom surface of the magnetic shell, and the second magnetic block is flatly embedded in the top surface of the mounting block. An electric telescopic rod is fixedly connected to the bottom of the mounting block, and the extraction tube is connected to the drive end of the electric telescopic rod. A connecting tube is connected to one side of the extraction tube, and a solenoid valve is mounted on the connecting tube. A telescopic joint is connected to one end of the connecting tube, and a negative pressure tube is connected to one end of the telescopic joint. A connector is connected to the top of the negative pressure tube. A connecting seat is fixedly connected to one side of the electric telescopic rod, and the negative pressure tube is fixedly connected to the connecting seat.

[0009] Furthermore, the connector is equipped with a one-way valve, and the connector fits into the connecting pipe.

[0010] The beneficial effects of this utility model are as follows: the sealing cap and the sealing groove are nested to form a physical isolation, and the closed flow channel of the extraction structure ensures that the entire process of culture and sampling is sterile. The magnetic shell allows the external magnetic field to drive the extraction module to move, avoiding the risk of contamination caused by physical contact. The electric telescopic rod controls the vertical lifting and lowering of the extraction tube. Combined with the negative pressure system, the cell fluid in the designated culture tank can be accurately extracted. Attached Figure Description

[0011] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] Figure 1 This is a schematic diagram of the main structure of an NK cell preparation and culture device according to an embodiment of the present invention;

[0013] Figure 2 This is a schematic diagram of the bottom shell of an NK cell preparation and culture device according to an embodiment of the present invention;

[0014] Figure 3 This is a schematic diagram of the culture structure of an NK cell preparation and culture device according to an embodiment of the present invention;

[0015] Figure 4 This is a schematic diagram of the extraction structure of an NK cell preparation and culture device according to an embodiment of the present invention.

[0016] In the picture:

[0017] 1. Bottom shell; 2. Culture structure; 201. Culture plate; 202. Culture tank; 203. Sealing groove; 204. Connecting ear; 3. Sealing structure; 301. Sealing cover; 302. Magnetic shell; 303. Connecting tube; 4. Extraction structure; 401. First magnetic block; 402. Holding block; 403. Press switch; 404. Guide block; 405. Mounting block; 406. Second magnetic block; 407. Electric telescopic rod; 408. Extraction tube; 409. Connecting tube; 410. Solenoid valve; 411. Expansion joint; 412. Negative pressure tube; 413. Connecting seat; 414. Connecting joint. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] According to an embodiment of the present invention, an NK cell preparation and culture apparatus is provided.

[0020] like Figure 1-4 As shown, the NK cell preparation and culture device according to an embodiment of the present invention includes a bottom shell 1, a culture structure 2 placed on the bottom shell 1, a sealing structure 3 covering the culture structure 2, and an extraction structure 4 connected to the sealing structure 3. The culture structure 2 achieves a relatively sealed anti-contamination culture function through the sealing structure 3, and achieves a sealed extraction function through the extraction structure 4. The extraction structure 4 serves as the device base for fixing the culture structure 2 and is made of corrosion-resistant stainless steel.

[0021] like Figure 1-4 As shown, the culture structure 2 includes a culture plate 201, a culture tank 202, a sealing groove 203, and connecting ears 204. The culture tank 202 is evenly distributed inside the culture plate 201. The sealing groove 203 is opened on the inner side of the top of the culture plate 201. The connecting ears 204 are fixedly connected around the culture plate 201. The culture plate 201 fits onto the bottom shell 1. Multiple cylindrical culture tanks 202 are evenly distributed on the surface of the culture plate 201 for placing cell culture medium. The sealing groove 203 is located on the inner side of the top of the culture plate 201 and forms a nested sealing structure with the sealing cap 301. The connecting ears 204 are symmetrically distributed around the culture plate 201 to facilitate the removal of the culture plate 201.

[0022] like Figure 1-4As shown, the sealing structure 3 includes a sealing cover 301, a magnetic shell 302, and a connecting pipe 303. The magnetic shell 302 is fixedly mounted on the sealing cover 301, and the connecting pipe 303 is provided on one side of the sealing cover 301. The sealing cover 301 is sealed and placed on the sealing groove 203, covering the culture plate 201. The edge of the sealing cover 301 is embedded in the sealing groove 203. The material is medical silicone to ensure airtightness. The magnetic shell 302 is fixed on the top of the sealing cover 301 and is made of non-magnetic transparent material to allow magnetic fields to penetrate. The connecting pipe 303 is connected to the side wall of the sealing cover 301 and is used to connect the connecting pipe 414.

[0023] like Figure 1-4 As shown, the extraction structure 4 includes a first magnetic block 401, a gripping block 402, a push switch 403, a guide block 404, a mounting block 405, a second magnetic block 406, an electric telescopic rod 407, an extraction tube 408, a connecting tube 409, a solenoid valve 410, a telescopic joint 411, a negative pressure tube 412, a connecting seat 413, and a coupling 414. The gripping block 402 is fixedly connected to the first magnetic block 401, and the push switch 403 is mounted on the gripping block 402. Guide blocks 404 are fixedly connected around the first magnetic block 401, and a caster wheel is fixedly mounted at the bottom of the guide block 404. The caster wheel contacts the top of the magnetic shell 302. The second magnetic block 406 is mounted on the bottom surface of the magnetic shell 302 and is flush-fitted into the top surface of the mounting block 405. The electric telescopic rod 407 is fixedly connected to the bottom of the mounting block 405, and the drive end of the electric telescopic rod 407 is connected to... There is an extraction tube 408, one side of which is connected to a connecting tube 409. A solenoid valve 410 is installed on the connecting tube 409. One end of the connecting tube 409 is connected to a telescopic joint 411. One end of the telescopic joint 411 is connected to a negative pressure tube 412. The top end of the negative pressure tube 412 is connected to a connector 414. One side of the electric telescopic rod 407 is fixedly connected to a connecting seat 413. The connecting seat 413 is fixedly connected to the negative pressure tube 412. The connector 414 is equipped with a one-way valve. The connector 414 fits into the connector tube 303. The first magnetic block 401 and the second magnetic block 406 form a magnetic positioning module. The first magnetic block 401 and the second magnetic block 406 achieve non-contact adsorption through the magnetic shell 302 to ensure that the extraction device has no physical contact with the culture structure 2. The electric telescopic rod 407 and the extraction tube 408 form a driving module. The electric telescopic rod 407 drives the extraction tube 408 to rise and fall vertically.

[0024] like Figure 1-4As shown, after the negative pressure pipe 412 is connected to the connecting pipe 303 via the connector 414, a negative pressure pump can be used to draw negative pressure into the negative pressure pipe 412. Then, the connector 414 is disconnected and the solenoid valve 410 is kept closed, so that the negative pressure pipe 412 maintains a certain negative pressure. The expansion joint 411 is used to compensate for pipeline displacement. The connector 414 has a built-in one-way valve, which forms a closed flow channel after being inserted with the connecting pipe 303, and then connects to the cell extraction device. The external interface of the connector 414 extracts the cell fluid drawn into the negative pressure pipe 412. The press switch 403 controls the electric telescopic rod 407 wirelessly. The electric telescopic rod 407 is equipped with a battery.

[0025] To facilitate understanding of the above-mentioned technical solutions of this utility model, the working principle or operation method of this utility model in actual process will be described in detail below.

[0026] With the help of the above-mentioned technical solution of this utility model, NK cell culture medium is injected into culture tank 202, and sealing cap 301 is pressed with culture plate 201 through sealing groove 203 to form a sterile environment. Magnetic shell 302 allows external magnetic field to act on second magnetic block 406, but physically isolates the inner and outer space to avoid contamination.

[0027] The operator holds the grip block 402. The magnetic shell 302 has a positioning ring that matches the position of the culture tank 202. The first magnetic block 401, through magnetic attraction, moves the mounting block 405 connected to the second magnetic block 406 and its electric telescopic rod 407 on the magnetic shell 302, positioning it on the culture tank 202 containing the cell solution to be extracted. Then, pressing the switch 403 triggers the electric telescopic rod 407 to push down, causing the extraction tube 408 to extend into the culture tank 202. The solenoid valve 410 opens, connecting the negative pressure tube 412 to the extraction tube 408, thereby extracting the cell solution from the culture tank 202 into the negative pressure tube 412. Once the extraction is complete, the solenoid valve 410 is closed. Then, the gripping block 402 is moved to align the connector 414 with the connector 303. The outer connector of the connector 303 is then connected to the cell fluid extraction device. The cell fluid is then extracted from the negative pressure tube 412. This extraction method can extract cell base fluid from any culture tank 202, and the process is a sealed extraction. During the extraction, the sealing cap 301 and the culture plate 201 remain sealed, and the sample is output unidirectionally only through the connector 303 to prevent external microorganisms from entering. The universal wheel at the bottom of the guide block 404 assists the extraction structure 4 to move smoothly along the surface of the magnetic shell 302, reducing friction loss.

[0028] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An NK cell preparation and culture device, characterized in that, It includes a bottom shell (1), a culture structure (2) is placed on the bottom shell (1), a sealing structure (3) is covered on the culture structure (2), and an extraction structure (4) is connected to the sealing structure (3). The culture structure (2) achieves a relatively sealed anti-pollution culture function through the sealing structure (3), and achieves a sealed extraction function of the culture structure (2) through the extraction structure (4).

2. The NK cell preparation and culture apparatus according to claim 1, characterized in that, The culture structure (2) includes a culture plate (201), a culture tank (202), a sealing groove (203), and a connecting ear (204). The culture tank (202) is evenly provided inside the culture plate (201). The sealing groove (203) is opened on the inner side of the top of the culture plate (201). The connecting ear (204) is fixedly connected around the culture plate (201). The culture plate (201) fits into the bottom shell (1).

3. The NK cell preparation and culture device according to claim 2, characterized in that, The sealing structure (3) includes a sealing cover (301), a magnetic shell (302), and a connecting pipe (303). The magnetic shell (302) is fixedly provided on the sealing cover (301), and the connecting pipe (303) is provided on one side of the sealing cover (301). The sealing cover (301) is sealed and placed on the sealing groove (203).

4. The NK cell preparation and culture device according to claim 3, characterized in that, The extraction structure (4) includes a first magnetic block (401), a gripping block (402), a push switch (403), a guide block (404), a mounting block (405), a second magnetic block (406), an electric telescopic rod (407), an extraction tube (408), a connecting tube (409), a solenoid valve (410), an expansion joint (411), a negative pressure tube (412), a connecting seat (413), and a coupling (414). The gripping block (402) is fixedly connected to the first magnetic block (401), and the push switch (403) is provided on the gripping block (402). The guide block (404) is fixedly connected around the first magnetic block (401), and a universal wheel is fixedly provided at the bottom of the guide block (404).

5. The NK cell preparation and culture apparatus according to claim 4, characterized in that, The universal wheel contacts the top of the magnetic shell (302). The bottom surface of the magnetic shell (302) is provided with a second magnetic block (406). The second magnetic block (406) is flatly embedded in the top surface of the mounting block (405). The bottom end of the mounting block (405) is fixedly connected to an electric telescopic rod (407).

6. The NK cell preparation and culture apparatus according to claim 5, characterized in that, The drive end of the electric telescopic rod (407) is connected to a pull tube (408), one side of the pull tube (408) is connected to a connecting tube (409), a solenoid valve (410) is installed on the connecting tube (409), and one end of the connecting tube (409) is connected to a telescopic joint (411).

7. The NK cell preparation and culture apparatus according to claim 6, characterized in that, One end of the expansion joint (411) is connected to the negative pressure pipe (412), and the top end of the negative pressure pipe (412) is connected to the connector (414).

8. The NK cell preparation and culture apparatus according to claim 7, characterized in that, A connecting seat (413) is fixedly connected to one side of the electric telescopic pole (407), and the connecting seat (413) is fixedly connected to the negative pressure pipe (412).

9. The NK cell preparation and culture apparatus according to claim 8, characterized in that, The connector (414) is equipped with a check valve and the connector (414) fits into the connector (303).

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