Leafhopper hemolymph collection device

Abstract

This utility model relates to the field of insect hemolymph collection technology, and discloses a leafhopper hemolymph collection device, including a collection tube, a rubber stopper at the opening of the collection tube, a negative pressure cylinder, a liquid collection tube with an insertion needle at one end, and a positioning component for positioning the collection tube. The other end of the liquid collection tube passes through the rubber stopper and is connected to the collection tube. The rubber stopper is tightly fitted to the opening of the collection tube. An air inlet is opened at the upper part of the negative pressure cylinder, and a bent tube is fixedly inserted into the air inlet of the negative pressure cylinder. The bent tube is fixedly inserted into the rubber stopper and is connected to the collection tube. The positioning component includes an upper sleeve with an opening at the lower end, a lower sleeve with an opening at the upper end, and multiple arc-shaped positioning blocks made of elastic rubber. The purpose is to provide a device for collecting leafhopper hemolymph and to solve the problem that existing insect hemolymph extraction devices require insertion and removal of the suction hose and liquid collection hose on the sealing cap during use, resulting in a complex structure and inconvenience in use.

Description

Technical Field

[0001] This utility model belongs to the field of insect hemolymph collection technology, specifically relating to a hemolymph collection device for leafhoppers. Background Technology

[0002] Leafhoppers belong to the order Hemiptera, suborder Auchenorrhyncha, and family Cicadellidae, and are widely distributed across the six major zoogeographical regions of the world. They are a group of vector insects that seriously damage crops. They feed on the phloem sap of plants, transmitting plant pathogens while sucking the sap, and are a major cause of outbreaks and spreads of plant diseases in the field. Pathogens circulate and infect within the leafhopper via the hemolymphatic system. The collection and study of leafhopper hemolymph is a crucial breakthrough in understanding pathogen transmission and the interaction between pathogens and vector insects. Previous literature has not described any collection devices for leafhopper hemolymph.

[0003] Chinese patent CN 216495298 U discloses a device for extracting hemolymph from worker bees, including an air extraction cylinder. The air inlet of the cylinder is connected to one end of an extraction hose, and the other end of the extraction hose is connected to a low-temperature collection unit. The low-temperature collection unit is also connected to one end of a collection hose, and the other end of the collection hose is connected to the tail end of a collection needle. This solves the problems of cumbersome and complex operation steps, long time consumption, susceptibility to contamination, and low quality and content of the collected hemolymph in existing bee hemolymph extraction technologies. However, this hemolymph extraction device requires inserting and removing the extraction hose and collection hose from the sealing cap during use, making the structure relatively complex and inconvenient to use. Furthermore, leafhoppers and bees have significant differences in biological characteristics and size. Therefore, we propose a collection device for leafhopper hemolymph research to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a device for collecting hemolymph from leafhoppers and to solve the problem that existing insect hemolymph extraction devices require inserting and removing the suction hose and collection hose on the sealing cap during use, resulting in a complex structure and inconvenience in use.

[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by this utility model is as follows:

[0006] A leafhopper hemolymph collection device includes a collection tube, a rubber stopper at the opening of the collection tube, a negative pressure cylinder, a liquid collection tube with an insertion needle at one end, and a positioning component for positioning the collection tube. The other end of the liquid collection tube passes through the rubber stopper and is connected to the collection tube. The rubber stopper is tightly fitted to the opening of the collection tube. An air inlet is provided at the upper part of the negative pressure cylinder. A bent tube is fixedly inserted into the air inlet of the negative pressure cylinder. The bent tube is fixedly inserted into the rubber stopper and is connected to the collection tube.

[0007] Further specifying, the positioning assembly includes an upper sleeve with an opening at its lower end, a lower sleeve with an opening at its upper end, and multiple arc-shaped positioning blocks made of elastic rubber. The rubber plug is fixedly installed on the top of the inner wall of the upper sleeve. The collection tube passes through the upper end of the upper sleeve. The lower opening of the upper sleeve has an internal thread. The lower sleeve is threadedly connected to the lower end of the upper sleeve. The multiple arc-shaped positioning blocks are fixedly installed inside the lower sleeve. When the collection tube is located inside the lower sleeve, the arc-shaped positioning blocks contact the outer wall of the collection tube. This structural design, with multiple arc-shaped positioning blocks working together, can position the collection tube, clamping it at the center of the lower sleeve.

[0008] Furthermore, a compression spring is fixedly installed at the bottom of the inner wall of the lower sleeve, and a tray is fixedly installed at the upper end of the compression spring. A positioning groove is formed at the center of the upper end of the tray, and the upper end of the tray is coated with a rubber layer. With this structural design, the compression spring, in conjunction with the tray, not only positions the collection tube but also lifts the collection tube upward, maintaining a sealed relationship between the collection tube and the rubber stopper.

[0009] Further specifying, a piston is movably installed inside the negative pressure cylinder, and a push rod is fixedly installed at the upper end of the piston. The upper end of the push rod penetrates through the upper end of the negative pressure cylinder, and a sealing ring is provided at the penetration point. A pressing block is fixedly installed at the upper end of the push rod. The negative pressure cylinder is movably inserted into the upper sleeve. The lower end of the negative pressure cylinder has an air outlet, and an air outlet pipe is fixedly installed at the air outlet. The end of the air outlet pipe penetrates through the upper sleeve. With this structural design, when the piston moves downward by the pressing block and the push rod, air is discharged outward through the air outlet pipe, and the upper part of the negative pressure cylinder is in a negative pressure state. Combined with the collection tube, the hemolymph of the leafhopper can be drawn into the collection tube, making it more convenient to use.

[0010] Furthermore, the upper sleeve has an input hole, and a movable plug is movably inserted into the input hole. This structural design allows cold air or coolant to be supplied between the upper and lower sleeves through the input hole, while the movable plug helps to retain the cold air and coolant inside.

[0011] The utility model adopting the above technical solution has the following advantages:

[0012] This invention features a positioning component for easy positioning of the collection tube, eliminating the need to hold the tube by hand. The needle is inserted into the leafhopper's body, and the collection tube is brought to a negative pressure state using a negative pressure cylinder and a bent tube. Combined with the collection tube, the leafhopper's hemolymph can be collected into the collection tube. The structure is simple and easy to use. Attached Figure Description

[0013] This utility model can be further illustrated by the non-limiting embodiments given in the accompanying drawings;

[0014] Figure 1 This is a schematic diagram of the structure of the leafhopper hemolymph collection device of this utility model;

[0015] Figure 2 This is a cross-sectional structural schematic diagram of the leafhopper hemolymph collection device of this utility model;

[0016] Figure 3 for Figure 2 Enlarged structural diagram at point A;

[0017] Figure 4 for Figure 2 Enlarged structural diagram at point B;

[0018] Figure 5 This is a cross-sectional view of the upper sleeve in the leafhopper hemolymph collection device of this utility model.

[0019] The symbols for the main components are explained below:

[0020] 1. Collection tube; 2. Rubber stopper;

[0021] 3. Negative pressure cylinder; 31. Bent pipe; 32. Piston; 33. Push rod; 34. Air outlet pipe;

[0022] 4. Liquid collecting tube;

[0023] 5. Install the sleeve;

[0024] 51. Lower sleeve; 511. Compression spring; 512. Tray;

[0025] 52. Arc-shaped positioning block;

[0026] 6. Input hole; 61. Movable plug. Detailed Implementation

[0027] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that similar or identical parts are referred to by the same reference numerals in the drawings or description. Implementations not shown or described in the drawings are forms known to those skilled in the art. Furthermore, directional terms mentioned in the embodiments, such as "up," "down," "top," "bottom," "left," "right," "front," and "back," are only for reference to the directions in the drawings and are not intended to limit the scope of protection of the present invention.

[0028] like Figures 1-5 As shown, the leafhopper hemolymph collection device of this utility model includes a collection tube 1, a rubber stopper 2 set at the opening of the collection tube 1, a negative pressure cylinder 3, a liquid collection tube 4 with a needle inserted at one end, and a positioning component for positioning the collection tube 1. The other end of the liquid collection tube 4 passes through the rubber stopper 2 and is connected to the collection tube 1. The rubber stopper 2 is tightly fitted to the opening of the collection tube 1. An air inlet is opened at the upper part of the negative pressure cylinder 3. A bent tube 31 is fixedly inserted into the air inlet of the negative pressure cylinder 3. The bent tube 31 is fixedly inserted into the rubber stopper 2 and is connected to the collection tube 1.

[0029] The positioning assembly includes an upper sleeve 5 with an opening at the lower end, a lower sleeve 51 with an opening at the upper end, and multiple arc-shaped positioning blocks 52 made of elastic rubber. A rubber plug 2 is fixedly installed on the top of the inner wall of the upper sleeve 5. A liquid collection tube 4 passes through the upper end of the upper sleeve 5. The lower end of the upper sleeve 5 has an internal thread. The lower sleeve 51 is threaded to the lower end of the upper sleeve 5. Multiple arc-shaped positioning blocks 52 are fixedly installed inside the lower sleeve 51. There is a gap between adjacent arc-shaped positioning blocks 52 so that cold air can flow inside the lower sleeve 51 and can smoothly cool the part of the collection tube 1 located inside the lower sleeve 51. When the collection tube 1 is located inside the lower sleeve 51, the arc-shaped positioning blocks 52 are in contact with the outer wall of the collection tube 1.

[0030] A compression spring 511 is fixedly installed on the bottom of the inner wall of the lower sleeve 51. A tray 512 is fixedly installed on the upper end of the compression spring 511. A positioning groove is opened in the center of the upper end of the tray 512. When the collection tube 1 is inserted into the lower sleeve 51, the lower end of the collection tube 1 will slide into the positioning groove, which makes the support of the collection tube 1 more stable. The upper end of the tray 512 is coated with a rubber layer, which can increase the friction with the end of the tube when it comes into contact with the collection tube 1. Because of its elasticity, it can protect the collection tube 1.

[0031] A piston 32 is movably installed inside the negative pressure cylinder 3. A push rod 33 is fixedly installed on the upper end of the piston 32. The upper end of the push rod 33 passes through the upper end of the negative pressure cylinder 3, and a sealing ring is provided at the passage. A pressing block is fixedly installed on the upper end of the push rod 33. The negative pressure cylinder 3 is movably inserted into the upper sleeve 5. The lower end of the negative pressure cylinder 3 is provided with an air outlet. An air outlet pipe 34 is fixedly installed on the air outlet. The end of the air outlet pipe 34 passes through the upper sleeve 5. An inlet hole 6 is opened on the upper sleeve 5. A movable plug 61 is movably inserted into the inlet hole 6. Cold air or coolant can be delivered into the upper sleeve 5 and the lower sleeve 51 through the inlet hole 6 to keep the collecting pipe 1 at a low temperature.

[0032] The method of using this utility model is as follows:

[0033] When in use, align the opening of the collection tube 1 with the rubber stopper 2, apply force to assemble the collection tube 1 onto the upper sleeve 5, hold the lower sleeve 51, align the center of the lower sleeve 51 with the end of the collection tube 1, so that the collection tube 1 extends into the lower sleeve 51 and is clamped by the arc-shaped positioning block 52.

[0034] When the lower sleeve 51 contacts the lower end of the upper sleeve 5, rotate the lower sleeve 51 and thread the lower sleeve 51 to the lower end of the upper sleeve 5. At this time, the tray 512 generates an upward supporting force on the collection tube 1, keeping the tube opening of the collection tube 1 in close contact with the rubber stopper 2.

[0035] When it is necessary to collect leafhopper hemolymph, open the movable plug 61 to fill the space between the upper sleeve 5 and the lower sleeve 51 with cold air or coolant. Then, control the insertion needle to pierce the leafhopper's body. Press the push rod 33 and piston 61 by pressing the pressing block. The air in the negative pressure cylinder 3 will be discharged through the air outlet 34, so that the collection tube 1 is in a negative pressure state, thereby drawing the leafhopper hemolymph into the collection tube 1 and completing the collection of leafhopper hemolymph.

[0036] The foregoing has provided a detailed description of the leafhopper hemolymph collection device provided by this utility model. The specific embodiments are described only to aid in understanding the method and core concept of this utility model. It should be noted that those skilled in the art can make various improvements and modifications to this utility model without departing from its principles, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

Claims

1. A leafhopper hemolymph collection device, comprising a collection tube (1), a rubber stopper (2) disposed at the opening of the collection tube (1), a negative pressure cylinder (3), a collection tube (4) with an insertion needle at one end, and a positioning component for positioning the collection tube (1), wherein the other end of the collection tube (4) passes through the rubber stopper (2) and is connected to the collection tube (1), and the rubber stopper (2) is tightly fitted to the opening of the collection tube (1), characterized in that: The upper part of the negative pressure cylinder (3) is provided with an air inlet end. A bent tube (31) is fixedly inserted into the air inlet end of the negative pressure cylinder (3). The bent tube (31) is fixedly inserted into the rubber plug (2) and connected to the collection tube (1).

2. The leafhopper hemolymph collection device according to claim 1, characterized in that: The positioning assembly includes an upper sleeve (5) with an opening at the lower end, a lower sleeve (51) with an opening at the upper end, and multiple arc-shaped positioning blocks (52) made of elastic rubber. The rubber plug (2) is fixedly installed on the top of the inner wall of the upper sleeve (5). The liquid collection tube (4) passes through the upper end of the upper sleeve (5). The lower end opening of the upper sleeve (5) is provided with an internal thread. The lower sleeve (51) is threadedly connected to the lower end of the upper sleeve (5). Multiple arc-shaped positioning blocks (52) are fixedly installed inside the lower sleeve (51). When the collection tube (1) is located inside the lower sleeve (51), the arc-shaped positioning blocks (52) are in contact with the outer wall of the collection tube (1).

3. The leafhopper hemolymph collection device of claim 2, wherein: A compression spring (511) is fixedly installed on the bottom of the inner wall of the lower sleeve (51), and a tray (512) is fixedly installed on the upper end of the compression spring (511). A positioning groove is provided at the center of the upper end of the tray (512), and the upper end of the tray (512) is coated with a rubber layer.

4. The leafhopper hemolymph collection device of claim 2, wherein: A piston (32) is movably installed inside the negative pressure cylinder (3). A push rod (33) is fixedly installed at the upper end of the piston (32). The upper end of the push rod (33) passes through the upper end of the negative pressure cylinder (3), and a sealing ring is provided at the passage. A pressing block is fixedly installed at the upper end of the push rod (33). The negative pressure cylinder (3) is movably inserted into the upper sleeve (5). An air outlet is provided at the lower end of the negative pressure cylinder (3). An air outlet pipe (34) is fixedly installed at the air outlet. The end of the air outlet pipe (34) passes through the upper sleeve (5).

5. The leafhopper hemolymph collection device of claim 2, wherein: An input hole (6) is provided on the upper sleeve (5), and a movable plug (61) is movably inserted into the input hole (6).

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