Outdoor portable DNA enrichment device for aquatic environments

Abstract

This utility model provides an outdoor portable aquatic DNA enrichment device, belonging to the technical field of aquatic DNA enrichment devices. The utility model includes a main body with a pump inside. A water inlet pipe is located on one side of the main body. A drain pipe is inserted into the top of the enrichment reagent kit. Two handles are fixedly connected to the top of the main body. A positioning device is provided on the outer surface of each handle. The positioning device includes a U-shaped rod, and a locking block is slidably connected to one end of the inner wall of the pressure rod. By setting the positioning device, after the drain pipe is connected to the top of the enrichment reagent kit, the end of the drain pipe near the enrichment reagent kit is wrapped around the outer surface of the pressure rod, and the pressure rod restricts the position of the drain pipe outside the pressure rod. This minimizes the risk of the drain pipe loosening and falling off at the end near the enrichment reagent kit during use, thus improving the stability of the enrichment device during operation.

Description

Technical Field

[0001] This utility model relates to the technical field of DNA enrichment devices for aquatic environments, and in particular to an outdoor portable DNA enrichment device for aquatic environments. Background Technology

[0002] Aquatic DNA enrichment devices are equipment used to extract and enrich environmental DNA from water bodies, and are widely used in fields such as aquatic biodiversity monitoring, invasive species detection, and ecological health assessment.

[0003] When using an aquatic DNA enrichment device, the device's pumping pipe is placed into the water area to be sampled. Water is drawn into the device by an internal pump and output through different outlet pipes. The enrichment kit is then used to enrich the eDNA sample. The enrichment kit and the outlet pipe at the top of the enrichment device are usually connected by threads. The drain pipe is usually inserted directly into the outside of the top of the kit, with the other end connected to the sampling bag. During the drainage process, if the drain pipe is pulled or the pump is unstable, causing a sudden increase in drainage pressure, the connection between the drain pipe and the top of the enrichment kit may loosen, resulting in water leakage and affecting the enrichment process. Utility Model Content

[0004] The purpose of this invention is to solve the problem that when the drainage pipe is stretched or the pump is unstable during the drainage process, the connection between the drainage pipe and the top of the enrichment reagent kit may become loose, causing water sample leakage and affecting the enrichment process. This invention proposes an outdoor portable DNA enrichment device for aquatic environments.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: an outdoor portable aquatic DNA enrichment device, comprising a body, a pump installed inside the body, a water inlet pipe installed on one side of the body, and several connecting pipes fixedly connected to the top of the body, the outer surface of the connecting pipes being threaded, the two ends of the pump inside the body being connected to one side of the water inlet pipe and the bottom end of each connecting pipe respectively through pipes, an enrichment reagent kit being threadedly connected to the outer surface of the connecting pipes, a drain pipe being inserted into the top of the enrichment reagent kit, and two handles fixedly connected to the top of the body, the outer surface of the handles being provided with a positioning device that can restrict the position of the drain pipe at the top of the body.

[0006] The effect achieved by the above components is as follows: When using the water environment DNA enrichment device, connect the water pumping pipe to the water inlet pipe on one side of the device, then connect each enrichment kit to the connecting pipe thread at the top of the device, insert several drain pipes on the outside of the top of the enrichment kit, and connect the other end of the drain pipe to the water sample collection bag. Place one end of the water pumping pipe into the water source, and control the operation of the pump inside the device through the control panel on one side of the device. The pump draws water into the device and outputs it through the connecting pipes. When the water sample passes through the enrichment kit, the eDNA in the water sample can be enriched and sampled through the filter inside the enrichment kit. Then the water will enter the sampling bag through the drain pipe. After sampling is completed, stop the operation of the pump inside the device and remove the water pumping pipe, drain pipe and enrichment kit.

[0007] Preferably, the positioning device includes a U-shaped rod, the bottom end of which is fixedly connected to the top ends of two handles. A stop bar is fixedly connected to the outer surface of the U-shaped rod, and the outer surface of the stop bar has several grooves. A rotating shaft is rotatably connected to one end of the U-shaped rod, and a pressure rod is fixedly connected to the outer surface of the rotating shaft. A locking block is slidably connected to one end of the inner wall of the pressure rod. A spring is provided on one side of the locking block, and the spring is installed inside the pressure rod to limit the position of the locking block at one end of the pressure rod. A slot is provided at the end of the U-shaped rod away from the rotating shaft.

[0008] The effect achieved by the above components is as follows: When using the enrichment device, after connecting the drain tube to the top of the enrichment reagent kit, the end of the drain tube away from the enrichment reagent kit is passed through the bottom of the U-shaped rod and the stop rod. Pulling the pressure rod and rotating it upwards via the rotating shaft causes the drain tube to pass through the top of the stop rod in the opposite direction and wrap around the outer surface of the stop rod. Then, push the pressure rod to rotate in the direction of the U-shaped rod, press one end of the locking block to make the locking block enter the interior of the pressure rod and compress the spring. When the pressure rod and the U-shaped rod are parallel to each other, the compressed spring inside the pressure rod returns to its original state and pushes the locking block outwards, inserting the locking block into the slot. This fixes the position of the pressure rod at the top of the U-shaped rod, so that the groove on the outer surface of the pressure rod presses against the outside of the drain tube, further restricting the position of the drain tube at the top of the device and preventing the drain tube from becoming loose as much as possible.

[0009] Preferably, the two sides of the stop bar are covered with rubber sheets, and the outer edge of the rubber sheets is arc-shaped.

[0010] The effect achieved by the above components is that by covering the two sides of the stop bar with arc-shaped rubber sheets, the surface of the drain pipe is not easily scratched by the edge of the stop bar when it is wrapped around the outer surface of the stop bar.

[0011] Preferably, one end of the rotating shaft is provided with a torsion spring, and the two ends of the torsion spring are fixedly connected to one side of the rotating shaft and one side of the U-shaped rod, respectively.

[0012] The effect achieved by the above components is that by setting torsion springs, the position of the rotating shaft and the pressure rod can be further restricted, and the angle between the pressure rod and the U-shaped rod can be adjusted to facilitate the locking of the block into the slot.

[0013] Preferably, an operating rod is fixedly connected to the top of the card block, and the operating rod slides on one end of the inner wall of the pressure rod.

[0014] The effect achieved by the above components is that squeezing the outer surface of the operating lever makes it easier to push the operating lever to control the movement of the locking block inside the pressure rod, so that the locking block is disengaged from the slot, making it easier to rotate the pressure rod to release the position restriction on the drain pipe.

[0015] Preferably, a storage device is provided on one side of the machine body. The storage device includes a slot box, which is fixed to one side of the machine body. Two drawers are slidably connected to the inner wall of the slot box, and the drawers have storage slots inside.

[0016] The effect achieved by the above components is as follows: when carrying the enrichment device, the two drawers can be pulled out by moving them inside the slot on one side of the device. Then, the excess spare enrichment reagent kits or enrichment reagent kits after sampling can be placed in the storage slot inside the drawer and pushed into the slot. This makes it convenient to store and carry the enrichment reagent kits and improves the convenience of using the enrichment device.

[0017] Preferably, a pull rod is fixedly connected to one side of the drawer, and the outer surface of the pull rod is U-shaped.

[0018] The effect achieved by the above components is that holding the outer surface of the U-shaped lever makes it easier to pull the lever and move the drawer.

[0019] Preferably, a limiting rod is fixedly connected to one side of the drawer, and the limiting rod is made of rubber.

[0020] The effect achieved by the above components is that when the drawer is pushed to move and enter the slot, one side of the rubber limiting rod will be attached to one side of the outer surface of the slot. The limiting rod can further secure the position of the drawer inside the slot, making it less likely to accidentally slide outward when the drawer is put into the slot.

[0021] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0022] In this invention, by setting a positioning device, after connecting the drain pipe to the top of the enrichment reagent kit, the end of the drain pipe near the enrichment reagent kit is wrapped around the outer surface of the baffle, and the position of the drain pipe outside the baffle is restricted by the pressure rod. This avoids the drain pipe from being pulled loose and falling off when using the enrichment device, thus affecting the normal progress of the enrichment process and improving the stability of the enrichment device during use. Attached Figure Description

[0023] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0024] Figure 2 This is a three-dimensional structural diagram of the body of this utility model;

[0025] Figure 3 This is a three-dimensional structural diagram of the slot box of this utility model;

[0026] Figure 4 This is a three-dimensional structural diagram of the U-shaped rod of this utility model;

[0027] Figure 5 This utility model Figure 4 A magnified three-dimensional structural diagram of point A.

[0028] Legend: 1. Body; 2. Positioning device; 3. Storage device; 4. Water inlet pipe; 5. Connecting pipe; 6. Enrichment reagent kit; 7. Drain pipe; 8. Handle; 21. U-shaped rod; 22. Stop bar; 23. Rotating shaft; 24. Pressure rod; 25. Groove; 26. Spring; 27. Locking block; 28. Locking slot; 29. ​​Torsion spring; 210. Operating lever; 31. Slot box; 32. Drawer box; 33. Storage slot; 34. Pull rod; 35. Limiting rod. Detailed Implementation

[0029] Example 1, as Figure 1-2As shown, the outdoor portable aquatic DNA enrichment device includes a body 1. A pump is installed inside the body 1. A water inlet pipe 4 is installed on one side of the body 1. Several connecting pipes 5 are fixedly connected to the top of the body 1. The outer surface of the connecting pipes 5 is threaded. The two ends of the pump inside the body 1 are connected to one side of the water inlet pipe 4 and the bottom of each connecting pipe 5 via pipes. An enrichment reagent kit 6 is threaded onto the outer surface of the connecting pipes 5. A drain pipe 7 is inserted into the top of the enrichment reagent kit 6. Two handles 8 are fixedly connected to the top of the body 1. The outer surface of the handles 8 is provided with a positioning device 2 that can restrict the position of the drain pipe 7 at the top of the body 1. When using the aquatic DNA enrichment device, the water inlet pipe is connected to the water inlet pipe 4 on one side of the body 1. Connect the water pipe 4, and then thread each enrichment kit 6 to the connecting pipe 5 at the top of the body 1. Insert several drain pipes 7 into the outside of the top of the enrichment kit 6, and connect the other end of the drain pipe 7 to the water sample collection bag. Place one end of the water pump into the water source. Control the operation of the pump inside the body 1 through the control panel on one side of the body 1. The pump draws water into the body 1 and outputs it through each connecting pipe 5. When the water sample passes through the enrichment kit 6, the eDNA in the water sample can be enriched and sampled through the filter inside the enrichment kit 6. Then the water will enter the sampling bag through the drain pipe 7. After sampling is completed, stop the operation of the pump inside the body 1 and remove the water pump, drain pipe and enrichment kit 6.

[0030] Reference Figure 1-5As shown, in this embodiment: the positioning device 2 includes a U-shaped rod 21, the bottom end of which is fixedly connected to the top ends of two handles 8. A stop bar 22 is fixedly connected to the outer surface of the U-shaped rod 21, and several grooves 25 are formed on the outer surface of the stop bar 22. A rotating shaft 23 is rotatably connected to one end of the U-shaped rod 21, and a pressure rod 24 is fixedly connected to the outer surface of the rotating shaft 23. A locking block 27 is slidably connected to one end of the inner wall of the pressure rod 24, and a spring 26 is provided on one side of the locking block 27. The spring 26 is installed on the pressure rod 24. The internal mechanism is used to restrict the position of the locking block 27 at one end of the pressure rod 24. A slot 28 is provided at the end of the U-shaped rod 21 away from the rotating shaft 23. When using the enrichment device, after connecting the drain pipe 7 to the top of the enrichment reagent kit 6, the end of the drain pipe 7 away from the enrichment reagent kit 6 is passed between the bottom end of the U-shaped rod 21 and the stop rod 22. The pressure rod 24 is pulled upwards via the rotating shaft 23, causing the drain pipe 7 to pass through the top of the stop rod 22 in the opposite direction and wrap around the outer surface of the stop rod 22. Then, the pressure rod 24 is pushed towards the U-shaped rod 24. Rotating lever 21 presses one end of the locking block 27, causing the locking block 27 to enter the interior of the pressure lever 24 and compress the spring 26. When the pressure lever 24 and the U-shaped lever 21 are parallel to each other, the compressed spring 26 inside the pressure lever 24 returns to its original state and pushes the locking block 27 outward, inserting the locking block 27 into the slot 28. This fixes the position of the pressure lever 24 at the top of the U-shaped lever 21, causing the groove 25 on the outer surface of the pressure lever 24 to press against the outside of the drain pipe 7, further restricting the position of the drain pipe 7 at the top of the body 1, and minimizing the possibility of the drain pipe 7 becoming loose. By setting the positioning device 2, after connecting the drain pipe 7 to the top of the enrichment reagent kit 6, the end of the drain pipe 7 near the enrichment reagent kit 6 is wrapped around the outer surface of the baffle 22, and the position of the drain pipe 7 outside the baffle 22 is restricted by the pressure lever 24, minimizing the possibility of the drain pipe 7 near the enrichment reagent kit 6 being pulled loose and falling off during the use of the enrichment device, thus affecting the normal progress of the enrichment process and improving the stability of the enrichment device during use.

[0031] Reference Figure 2-5 As shown in this embodiment: rubber sheets are covered on both sides of the baffle 22. The outer edge of the rubber sheet is arc-shaped. By covering the two sides of the baffle 22 with arc-shaped rubber sheets, the surface of the drain pipe 7 is not easily scratched by the edge of the baffle 22 when it is wrapped around the outer surface of the baffle 22. A torsion spring 29 is provided at one end of the rotating shaft 23. The two ends of the torsion spring 29 are fixedly connected to one side of the rotating shaft 23 and one side of the U-shaped rod 21, respectively. By setting the torsion spring 29, the position of the rotating shaft 23 and the pressure rod 24 can be further restricted, and the angle between the pressure rod 24 and the U-shaped rod 21 can be adjusted to facilitate the insertion of the locking block 27 into the slot 28.

[0032] Reference Figure 2-5As shown in this embodiment: the top of the locking block 27 is fixedly connected to an operating rod 210. The operating rod 210 slides on one end of the inner wall of the pressure rod 24. Pinch the outer surface of the operating rod 210 to push the operating rod 210 to control the locking block 27 to move inside the pressure rod 24, so that the locking block 27 is disengaged from the slot 28, and the pressure rod 24 can be rotated to release the position restriction on the drain pipe 7.

[0033] Reference Figure 1-3 As shown in this embodiment: a storage device 3 is provided on one side of the main body 1. The storage device 3 includes a slot 31, which is fixed to one side of the main body 1. Two drawers 32 are slidably connected to the inner wall of the slot 31. The drawers 32 have storage slots 33 inside. When carrying the enrichment device, the two drawers 32 can be pulled out by moving them inside the slot 31 from one side of the main body 1. Then, excess spare enrichment reagent kits 6 or enrichment reagent kits 6 after sampling can be placed in the storage slots 33 inside the drawers 32. The drawers 32 can then be pushed back into the slot 31 for easy storage and carrying of the enrichment reagent kits 6. To improve the convenience of using the enrichment device, a pull rod 34 is fixedly connected to one side of the drawer 32. The outer surface of the pull rod 34 is U-shaped. Holding the outer surface of the U-shaped pull rod 34 makes it easier to pull the pull rod 34 to move the drawer 32. A limiting rod 35 is fixedly connected to one side of the drawer 32. The limiting rod 35 is made of rubber. When the drawer 32 is pushed to move and enter the slot 31, one side of the rubber limiting rod 35 will fit against one side of the outer surface of the slot 31. The limiting rod 35 can further secure the position of the drawer 32 inside the slot 31, making it less likely for the drawer 32 to slide out accidentally when it is entered into the slot 31.

[0034] Working principle: When using the aquatic DNA enrichment device, connect the water pump to the water inlet pipe 4 on one side of the main body 1, and then thread each enrichment kit 6 to the connecting pipe 5 at the top of the main body 1. Insert several drain pipes 7 into the outer side of the top of the enrichment kit 6, and pass the end of the drain pipe 7 away from the enrichment kit 6 through the space between the bottom of the U-shaped rod 21 and the stop rod 22. Pull the pressure rod 24 to rotate upward through the rotating shaft 23, so that the drain pipe 7 passes through the top of the stop rod 22 in the opposite direction and wraps around the stop rod. 22. On the outer surface, push the pressure rod 24 to rotate towards the U-shaped rod 21, press one end of the locking block 27 so that the locking block 27 enters the interior of the pressure rod 24 and compresses the spring 26. When the pressure rod 24 and the U-shaped rod 21 are parallel to each other, the compressed spring 26 inside the pressure rod 24 returns to its original state and pushes the locking block 27 outward, inserting the locking block 27 into the slot 28, fixing the pressure rod 24 at the top of the U-shaped rod 21, so that the groove 25 on the outer surface of the pressure rod 24 presses against the outside of the drain pipe 7. The position of the drain pipe 7 at the top of the body 1 is further restricted to prevent it from becoming loose. Then, the other end of the drain pipe 7 is connected to the water sample collection bag, and one end of the water pump is placed into the water source. The pump inside the body 1 is controlled by the control panel on one side of the body 1. The pump draws water into the body 1 and outputs it through each connecting pipe 5. When the water sample passes through the enrichment kit 6, the eDNA in the water sample can be enriched and sampled through the filter inside the enrichment kit 6. Then, the water will enter the sampling bag through the drain pipe 7. After sampling is completed, the pump inside the body 1 is stopped, and the water pump, drain pipe and enrichment kit 6 are removed. When carrying the enrichment device, the two draw boxes 32 can be pulled out by moving them inside the slot 31 on one side of the body 1. Then, the enrichment kit 6 after sampling is placed in the storage slot 33 inside the draw box 32 and pushed into the slot 31 for easy storage and carrying of the enrichment kit 6.

[0035] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model in any other way. Any person skilled in the art may use the disclosed technical content to make changes or modifications to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model, without departing from the scope of the utility model's technical solution, still fall within the protection scope of this utility model's technical solution. In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood through specific circumstances.

Claims

1. An outdoor portable water environment DNA enrichment device, comprising a body (1), characterized in that: The machine body (1) is equipped with a pump inside. A water inlet pipe (4) is provided on one side of the machine body (1). Several connecting pipes (5) are fixedly connected to the top of the machine body (1). The outer surface of the connecting pipes (5) is provided with threads. The two ends of the pump inside the machine body (1) are respectively connected to one side of the water inlet pipe (4) and the bottom end of each connecting pipe (5) through pipelines. An enrichment reagent kit (6) is threadedly connected to the outer surface of the connecting pipe (5). A drain pipe (7) is inserted into the top of the enrichment reagent kit (6). Two handles (8) are fixedly connected to the top of the machine body (1). The outer surface of the handles (8) is provided with a positioning device (2) that can restrict the position of the drain pipe (7) at the top of the machine body (1).

2. The portable outdoor aquatic environment DNA enrichment device according to claim 1, characterized in that: The positioning device (2) includes a U-shaped rod (21), the bottom end of which is fixedly connected to the top of two handles (8), a stop bar (22) is fixedly connected to the outer surface of the U-shaped rod (21), and a plurality of grooves (25) are provided on the outer surface of the stop bar (22). A rotating shaft (23) is rotatably connected to one end of the U-shaped rod (21), and a pressure rod (24) is fixedly connected to the outer surface of the rotating shaft (23). A locking block (27) is slidably connected to one end of the inner wall of the pressure rod (24), and a spring (26) is provided on one side of the locking block (27). The spring (26) is installed inside the pressure rod (24) to limit the position of the locking block (27) at one end of the pressure rod (24). A slot (28) is provided at the end of the U-shaped rod (21) away from the rotating shaft (23).

3. The portable outdoor aquatic environment DNA enrichment device according to claim 2, characterized in that: The two sides of the stop bar (22) are covered with rubber sheets, and the outer edge of the rubber sheets is arc-shaped.

4. The portable outdoor aquatic environment DNA enrichment device according to claim 3, characterized in that: One end of the rotating shaft (23) is provided with a torsion spring (29), and the two ends of the torsion spring (29) are fixedly connected to one side of the rotating shaft (23) and one side of the U-shaped rod (21), respectively.

5. The portable outdoor aquatic environment DNA enrichment device according to claim 4, characterized in that: The top of the card block (27) is fixedly connected to an operating rod (210), which slides on one end of the inner wall of the pressure rod (24).

6. The portable outdoor aquatic environment DNA enrichment device according to claim 5, characterized in that: A storage device (3) is provided on one side of the body (1). The storage device (3) includes a slot box (31). The slot box (31) is fixed on one side of the body (1). Two drawers (32) are slidably connected to the inner wall of the slot box (31). A storage slot (33) is provided inside the drawer (32).

7. The outdoor portable aquatic environment DNA enrichment device according to claim 6, characterized in that: A pull rod (34) is fixedly connected to one side of the drawer (32), and the outer surface of the pull rod (34) is U-shaped.

8. The outdoor portable aquatic environment DNA enrichment device according to claim 7, characterized in that: A limiting rod (35) is fixedly connected to one side of the drawer (32), and the limiting rod (35) is made of rubber.

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