Bioreactor for Rabies Virus Cell Culture Monitoring

Abstract

This utility model relates to the field of biopharmaceutical technology, and in particular to a bioreactor for monitoring rabies virus cell culture. It includes a tank and a detection mechanism. The detection mechanism is located on one side of the tank, and an assembly mechanism is located at one end of the tank. In this rabies virus cell culture monitoring bioreactor, the solution is first placed inside the tank through the detection mechanism, and a top cover is fixed to the tank via a threaded connection. The detection cover is used to seal the sample to be tested. An integrated head integrates a heating rod and is electrically connected to a battery pack to power the heating rod, thereby heating the sample inside the tank. The heating rod is located inside a sensor so that the sensor can monitor the sample status (such as gas, temperature, pressure, etc.) in real time when heated. The sensor is connected to the integrated head through a connector and a socket to form a closed detection circuit. The detection signal is transmitted to a display screen via the integrated head to display the detection result. If an abnormality occurs, an alarm light flashes.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceutical technology, and in particular to a bioreactor for monitoring rabies virus cell culture. Background Technology

[0002] Rabies virus cell culture monitoring refers to the process of real-time monitoring and regulation of key parameters in the culture environment, such as temperature, pH, dissolved oxygen concentration, cell density, and virus replication, during cell culture. The aim is to ensure the efficient, safe, and stable proliferation of rabies virus in host cells, providing reliable data support and culture conditions for vaccine production and virus research. Therefore, bioreactors for rabies virus cell culture monitoring are particularly needed.

[0003] However, existing bioreactors for monitoring rabies virus cell culture usually require opening the reactor when various data need to be detected. This can easily expose the culture environment to the outside world, increasing the risk of contamination. It can also cause fluctuations in parameters such as temperature, humidity, and gas concentration, affecting the normal growth of cells and viruses, reducing culture stability and yield. Furthermore, frequent operation increases human error and safety hazards. Utility Model Content

[0004] The purpose of this invention is to provide a bioreactor for monitoring rabies virus cell culture, in order to solve the problems mentioned in the background art. Existing bioreactors for monitoring rabies virus cell culture usually require opening the reactor when various data need to be detected, which easily exposes the culture environment to the outside world, increases the risk of contamination, and causes fluctuations in parameters such as temperature, humidity, and gas concentration, affecting the normal growth of cells and viruses, reducing culture stability and yield. In addition, frequent operation increases human error and safety hazards.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a bioreactor for monitoring rabies virus cell culture, comprising a tank and a detection mechanism, wherein the detection mechanism is provided on one side of the surface of the tank and a combination mechanism is provided at one end of the tank;

[0006] The detection mechanism includes a top cover, a placement slot, a detection cover, an integrated head, a threaded groove, a heating rod, a battery pack, a socket, an alarm light, a connecting head, a display screen, and a sensor. The top cover is threadedly connected to the top surface of the tank. A placement slot is formed in the central area of ​​the top cover. A detection cover is threadedly connected to the inside of the placement slot. An integrated head is installed at one end of the detection cover. A threaded groove is formed on one side of the surface of the detection cover. A heating rod is installed on one side of the integrated head. A battery pack is electrically connected to the top of the integrated head. A socket is formed on the side wall of the integrated head. An alarm light is installed on the side wall of the integrated head. A connecting head is threadedly connected to the inside of the threaded groove. A display screen is installed on the other side of the battery pack. A sensor is installed on one side of the connecting head.

[0007] Preferably, both the sensor and the heating rod are disposed inside the tank, with the heating rod disposed inside the sensor.

[0008] Preferably, there are six sets of sockets and alarm lights, and the six sets of sockets and alarm lights are distributed at equal angles on the surface of the integrated head, and are aligned one by one.

[0009] Preferably, one side of the connector is inserted into the socket, and the sensor is electrically connected to the integrated head through the connector and the socket.

[0010] Preferably, the threaded grooves are provided in six groups, and the six groups of threaded grooves are distributed at equal angles on the detection cover.

[0011] Preferably, the integrated head is electrically connected to the heating rod, and the display screen is electrically connected to the integrated head via a battery pack.

[0012] Preferably, the combined mechanism includes a discharge port, a mounting groove, a feed port, a rotating rod, a sealing cap, a motor, and blades. The discharge port is installed at the bottom of the tank. The mounting groove and the feed port are respectively opened on one side of the surface of the top cover. The rotating rod is fitted inside the mounting groove. The sealing cap is threaded inside the feed port. One side of the rotating rod is connected to the output end of the motor. Blades are installed on the surface of the rotating rod.

[0013] Preferably, the rotating rod and the mounting groove form a sliding structure, and the rotating rod and the blade rotate synchronously.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows: This bioreactor for monitoring rabies virus cell culture, through the setting of a detection mechanism and a combination mechanism, allows the detection mechanism to continuously monitor key parameters such as temperature, pH, dissolved oxygen, and cell density without opening the device, ensuring that the culture environment is always in the optimal state, thereby improving virus yield and stability. At the same time, the combination mechanism optimizes reaction conditions through stirring and material addition, and, in conjunction with a real-time reaction display screen, allows for understanding of real-time fluctuation data. Finally, the modular configuration enhances the flexibility of maintenance. Attached Figure Description

[0015] Figure 1 This is a side view of the appearance structure of this utility model;

[0016] Figure 2 This is a cross-sectional side view exploded structural diagram of some parts of the testing mechanism of this utility model;

[0017] Figure 3 This is a cross-sectional exploded side view of some parts of the combined mechanism of this utility model;

[0018] Figure 4 This utility model Figure 2 Enlarged structural diagram at point A in the middle;

[0019] Figure 5 This utility model Figure 3 Enlarged structural diagram at point B.

[0020] In the diagram: 1. Tank body; 2. Detection mechanism; 201. Top cover; 202. Placement slot; 203. Detection cover; 204. Integrated head; 205. Threaded groove; 206. Heating rod; 207. Battery pack; 208. Socket; 209. Alarm light; 210. Connecting head; 211. Display screen; 212. Sensor; 3. Assembly mechanism; 301. Discharge port; 302. Mounting slot; 303. Inlet; 304. Rotating rod; 305. Sealing cover; 306. Motor; 307. Blade. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-5This utility model provides a technical solution: a bioreactor for monitoring rabies virus cell culture, including a tank 1 and a detection mechanism 2. The detection mechanism 2 is provided on one side of the surface of the tank 1, and a combination mechanism 3 is provided at one end of the tank 1.

[0023] The testing mechanism 2 includes a top cover 201, a placement slot 202, a testing cover 203, an integrated head 204, a threaded groove 205, a heating rod 206, a battery pack 207, a socket 208, an alarm light 209, a connecting head 210, a display screen 211, and a sensor 212. The top cover 201 is threadedly connected to the top surface of the tank body 1. A placement slot 202 is formed in the central area of ​​the top cover 201. The testing cover 203 is threadedly connected to the inside of the placement slot 202. An integrated head 204 is installed at one end of the testing cover 203. 4. A threaded groove 205 is formed on one side of the surface of the detection cover 203. A heating rod 206 is installed on one side of the integrated head 204. A battery pack 207 is electrically connected to the top of the integrated head 204. An insertion hole 208 is formed on the side wall of the integrated head 204. An alarm light 209 is installed on the side wall of the integrated head 204. A connecting head 210 is threaded into the inside of the threaded groove 205. A display screen 211 is installed on the other side of the battery pack 207. A sensor 212 is installed on one side of the connecting head 210. The sensor is connected to the top cover. The setup of components 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, and 212 involves first placing the solution inside the tank 1, then fixing the top cover 201 to the top surface of the tank 1 via a threaded connection, and using the detection cover 203 to seal the sample to be tested. The integrated head 204 integrates the heating rod 206 and is electrically connected to the battery pack 207. The heating rod 206 is powered to heat the sample inside the container 1. The heating rod 206 is located inside the sensor 212 so that the sensor 212 can monitor the sample status (such as gas, temperature, pressure, etc.) in real time when it is heated. The sensor 212 is connected to the integrated head 204 through the connector 210 and the socket 208 to form a closed detection circuit. The detection signal is transmitted to the display screen 211 through the integrated head 204 to display the detection result. If an abnormality occurs, the alarm light 209 flashes to alarm.

[0024] Furthermore, both the sensor 212 and the heating rod 206 are located inside the container 1. The heating rod 206 is located inside the sensor 212. Through the setting of the heating rod 206, the heating rod 206 is used to heat the sample inside the container 1 to improve the reaction efficiency or stimulate the detection reaction, thereby providing effective detection conditions for the sensor 212.

[0025] Furthermore, six sets of sockets 208 and alarm lights 209 are provided. The six sets of sockets 208 and alarm lights 209 are distributed at equal angles on the surface of the integrated head 204 and are aligned one by one. Through the arrangement of sockets 208 and alarm lights 209, sockets 208 are used to connect sensors 212 and integrated head 204 to realize signal and power transmission connection. When an abnormality is detected, the alarm lights 209 emit an audible and visual alarm to remind operators to deal with it in time.

[0026] Furthermore, one side of the connector 210 is inserted into the socket 208. The sensor 212 is electrically connected to the integrated head 204 through the connector 210 and the socket 208. The connector 210 is used to connect the sensor 212 to the socket 208 to form an electrical path, thereby realizing the transmission of the sensing signal and the electrical connection with the integrated head 204.

[0027] Furthermore, six sets of threaded grooves 205 are provided, and the six sets of threaded grooves 205 are distributed at equal angles on the detection cover 203. Through the setting of the threaded grooves 205, the threaded grooves 205 are used to connect and fix the connecting head 210, ensuring a stable electrical connection and sealing stability between the sensor 212 and the integrated head 204.

[0028] Furthermore, the integrated head 204 is electrically connected to the heating rod 206, and the display screen 211 is electrically connected to the integrated head 204 through the battery pack 207. Through the configuration of the integrated head 204 and the display screen 211, the integrated head 204 integrates heating, signal processing and power connection functions, and is the core control unit of the detection mechanism 2. The display screen 211 is used to display the data collected by the sensor 212 in real time, so as to facilitate the operator to monitor the detection results.

[0029] Furthermore, the combined mechanism 3 includes a discharge port 301, a mounting groove 302, a feed port 303, a rotating rod 304, a sealing cover 305, a motor 306, and blades 307. The discharge port 301 is installed at the bottom of the tank body 1. The mounting groove 302 and the feed port 303 are respectively opened on one side of the surface of the top cover 201. The rotating rod 304 is fitted inside the mounting groove 302. The sealing cover 305 is threadedly connected inside the feed port 303. One side of the rotating rod 304 is connected to the output end of the motor 306. A sealing cover 305 is installed on the surface of the rotating rod 304. The blade 307, through the arrangement of the discharge port 301, mounting groove 302, inlet port 303, rotating rod 304, sealing cover 305, motor 306 and blade 307, allows for the addition of test liquid during the process. When it is necessary to add test liquid, the sample is added in a sealed manner through the inlet port 303. At the same time, the bottom of the tank body 1 is provided with a discharge port 301 for the discharge of sample or residual liquid after the test is completed. When it is necessary to mix the test liquid, the motor 306 is started. The motor 306 drives the rotating rod 304 to rotate, which in turn drives the blade 307 to rotate synchronously, so as to realize sample mixing or dynamic reaction.

[0030] Furthermore, the rotating rod 304 forms a sliding structure with the mounting groove 302 via the motor 306, and the rotating rod 304 and the blade 307 rotate synchronously. Through the arrangement of the rotating rod 304 and the blade 307, the rotating rod 304 is connected to the motor 306 and drives the rotation, thereby driving the blade 307 to rotate synchronously to stir the sample. The blade 307 rotates with the rotating rod 304 to stir the sample in the tank 1, thereby enhancing the mixing and reaction effect.

[0031] Working principle: First, the solution is placed inside the tank 1. Then, the top cover 201 is fixed to the top surface of the tank 1 through a threaded connection. The sample to be tested is sealed by the detection cover 203. The integrated head 204 integrates the heating rod 206 and is electrically connected to the battery pack 207 to power the heating rod 206, thereby heating the sample inside the tank 1. The heating rod 206 is located inside the sensor 212 so that the sensor 212 can monitor the sample status (such as gas, temperature, pressure, etc.) in real time when heated. The sensor 212 is connected to the integrated head 204 through the connector 210 and the socket 208 to form a closed detection circuit. The detection signal is transmitted to the display screen 211 through the integrated head 204 to display the detection result. If an abnormality occurs, the alarm light 209 flashes to sound an alarm.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A bioreactor for monitoring cell culture of rabies virus comprising a tank (1) and a detection mechanism (2), characterized in that: A detection mechanism (2) is provided on one side of the surface of the tank (1), and a combination mechanism (3) is provided at one end of the tank (1). The detection mechanism (2) includes a top cover (201), a placement slot (202), a detection cover (203), an integrated head (204), a threaded groove (205), a heating rod (206), a battery pack (207), a socket (208), an alarm light (209), a connecting head (210), a display screen (211), and a sensor (212). The top surface of the tank (1) is threadedly connected to the top cover (201). The center area of ​​the top cover (201) is provided with a placement slot (202). The inside of the placement slot (202) is threadedly connected to the detection cover (203). An integrated head is installed at one end of the detection cover (203). (204), a threaded groove (205) is provided on one side of the surface of the detection cover (203), a heating rod (206) is installed on one side of the integrated head (204), a battery pack (207) is electrically connected to the top of the integrated head (204), an insertion hole (208) is provided on the side wall of the integrated head (204), an alarm light (209) is installed on the side wall of the integrated head (204), a connecting head (210) is connected to the internal thread of the threaded groove (205), a display screen (211) is installed on the other side of the battery pack (207), and a sensor (212) is installed on one side of the connecting head (210).

2. The bioreactor for monitoring the cell culture of rabies virus according to claim 1, characterized in that: The sensor (212) and the heating rod (206) are both located inside the tank (1), and the heating rod (206) is located inside the sensor (212).

3. The bioreactor for monitoring the cell culture of rabies virus according to claim 1, characterized in that: The sockets (208) and the alarm lights (209) are provided in six sets. The six sets of sockets (208) and alarm lights (209) are distributed at equal angles on the surface of the integrated head (204) and are aligned one by one.

4. The bioreactor for monitoring rabies virus cell culture according to claim 1, characterized in that: One side of the connector (210) is inserted into the socket (208), and the sensor (212) is electrically connected to the integrated head (204) through the connector (210) and the socket (208).

5. The bioreactor for monitoring rabies virus cell culture according to claim 1, characterized in that: The threaded grooves (205) are provided in six groups, and the six groups of threaded grooves (205) are distributed at equal angles on the detection cover (203).

6. The bioreactor for monitoring rabies virus cell culture according to claim 1, characterized in that: The integrated head (204) is electrically connected to the heating rod (206), and the display screen (211) is electrically connected to the integrated head (204) through the battery pack (207).

7. The bioreactor for monitoring rabies virus cell culture according to claim 1, characterized in that: The combined mechanism (3) includes a discharge port (301), a mounting groove (302), a feed port (303), a rotating rod (304), a sealing cover (305), a motor (306), and blades (307). The discharge port (301) is installed at the bottom of the tank (1). The mounting groove (302) and the feed port (303) are respectively opened on one side of the surface of the top cover (201). The rotating rod (304) is fitted inside the mounting groove (302). The sealing cover (305) is threaded inside the feed port (303). One side of the rotating rod (304) is connected to the output end of the motor (306). Blades (307) are installed on the surface of the rotating rod (304).

8. The bioreactor for monitoring rabies virus cell culture according to claim 7, characterized in that: The rotating rod (304) forms a sliding structure with the mounting groove (302) via the motor (306), and the rotating rod (304) and the blade (307) rotate synchronously.

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