Biochemical incubator

Abstract

The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.

Description

Technical Field

[0001] This utility model belongs to the field of bioengineering equipment technology, and relates to a biochemical incubator. Background Technology

[0002] Biochemical incubators, as core equipment for maintaining a stable growth environment for biological samples, are widely used in life science research, pharmaceutical development, food testing, and other fields. They provide suitable culture conditions for cells, microorganisms, and other samples through precise control of parameters such as temperature, humidity, and carbon dioxide concentration. However, with the increasing sophistication of scientific research requirements for culture environments and the growing demand for high-throughput, long-term continuous culture, traditional biochemical incubators are gradually becoming insufficient to meet the needs of modern experiments in terms of both function and performance.

[0003] In actual production and use, existing biochemical incubators have revealed numerous problems. On the one hand, the control precision of temperature, humidity, and gas concentration is insufficient. Affected by uneven internal space distribution and external environmental interference, localized temperature fluctuations or humidity deviations can easily occur, leading to inconsistent sample growth and affecting the reliability of experimental data. On the other hand, the equipment lacks intelligent monitoring and control mechanisms, relying heavily on manual parameter setting and periodic inspections. It cannot detect environmental changes in real time and make dynamic adjustments, posing a risk of sample culture failure due to untimely handling of abnormal parameters. Furthermore, the sealing design of traditional incubators is not perfect, easily leading to gas leakage after long-term use, causing gas concentration imbalances within the chamber, and making maintenance inconvenient, increasing equipment operating costs.

[0004] Currently, some solutions exist to address the aforementioned issues. Patent CN202323196809.X proposes an intelligent biochemical incubator that uses a screw + slider + connecting pin structure to achieve rapid, equidistant expansion of partitions, shortening the time for placing large batches of samples and improving operational efficiency. However, its core improvement only addresses the mechanical structure of the partitions and does not involve key technologies such as temperature and humidity control and air purification, thus failing to solve the problem of culture environment stability. CN211645252U discloses a home fermentation chamber with semiconductor cooling and heating and ultrasonic humidification. It uses a conductive block + semiconductor cooling chip + radiator to achieve bidirectional temperature regulation and utilizes an ultrasonic atomizing plate to atomize room temperature water into micron-sized particles for humidification, avoiding the temperature interference of traditional heating and humidification. Its advantages include fast temperature control response, high humidity control accuracy, and improved safety through a 12V DC power supply. However, this solution is designed for home fermentation scenarios, lacks a professional-grade air filtration system, and the chamber structure lacks an insulation layer, failing to meet the cleanliness and energy consumption control requirements of biochemical culture. Utility Model Content

[0005] This invention provides a biochemical incubator that addresses the shortcomings of existing circulating hot water tanks in terms of temperature and humidity control and air purification, thus failing to provide a stable and suitable environment for cultivation.

[0006] To solve the above problems, the technical solution adopted by the utility model is as follows:

[0007] A biochemical incubator includes a base, a housing on the base, an incubator fixedly mounted inside the housing, and a conductive plate mounted on the incubator and fitted against it. A semiconductor cooling / heating module is mounted on the incubator and fixedly connected to the conductive plate. An air filtration system is located on one side of the housing and connected to the incubator via a circulation pipe. An ultrasonic humidifier is located below the semiconductor cooling / heating module and connected to the incubator via a pipe. A movable sealing door is mounted on the incubator and sealed to it. A movable door is located on the housing, and a controller is mounted on the movable door and fixedly mounted thereon. A gap is provided between the incubator and the housing.

[0008] The principle and advantages of this scheme are as follows:

[0009] The incubator utilizes a semiconductor cooling and heating module in conjunction with a conductive plate, leveraging the Peltier effect of semiconductors to achieve precise temperature control. Heat is rapidly transferred to the incubator via the conductive plate, ensuring temperature uniformity. An ultrasonic humidifier delivers water mist through pipes to the incubator, atomizing the water through high-frequency oscillation for efficient humidification, meeting the humidity requirements of the samples. An air filtration system, connected to the incubator via a circulation pipe, circulates and filters the air inside, removing impurities, microorganisms, and other contaminants to maintain a clean culture environment. The gap between the incubator and the main body forms an insulation layer, reducing external environmental interference with the internal temperature. Simultaneously, the sealed door fits tightly with the incubator to prevent gas leakage and ensure stable gas concentration. The controller integrates control of temperature, humidity, and air circulation modules, enabling intelligent adjustment. It can automatically start or stop components based on preset parameters to maintain stable culture conditions within the incubator.

[0010] Compared to existing technologies, this solution offers several advantages in temperature control. Existing technologies often employ single heating or cooling devices, resulting in low and uneven temperature control accuracy. This solution, however, combines a semiconductor cooling / heating module with a conductive plate to achieve high-precision temperature control of ±0.1℃. For example, in cell culture experiments, temperature fluctuations in traditional incubators can lead to abnormal cell growth, while the stable temperature environment of this solution effectively protects cell viability. Regarding humidity control, traditional incubators suffer from low humidification efficiency and uneven humidity distribution. This solution's ultrasonic humidifier can quickly stabilize humidity within ±2% of the set value, significantly improving the success rate of humidity-sensitive fungal culture experiments. The air filtration system is another major breakthrough. Existing technologies often lack efficient air purification capabilities, failing to meet aseptic culture requirements. This solution, through circulating filtration, can reduce the microbial content inside the chamber by over 90%, effectively preventing sample contamination. Furthermore, the gap design between the incubator and the chamber body, along with the sealed door structure, reduces gas leakage by 80% compared to traditional incubators, ensuring stable gas concentrations within the chamber. This advantage is particularly pronounced in cell metabolism experiments requiring precise carbon dioxide concentration control. The intelligent control of the controller has changed the traditional manual operation or simple remote monitoring mode. It can automatically adjust various parameters in real time according to environmental changes, avoid cultivation failure due to human negligence or data delay, and greatly improve the reliability and efficiency of the experiment. These are all unexpected technical effects that existing technologies cannot achieve.

[0011] Furthermore, the air filtration system includes a temperature and humidity regulating chamber and a filter, which are installed vertically stacked.

[0012] Furthermore, inclined pulsed light generators are provided on both sides of the top of the housing.

[0013] Furthermore, the inner wall of the incubator is coated with a nano-titanium dioxide photocatalytic coating.

[0014] Furthermore, a condenser dehumidifier is provided on the back of the incubator, and the condenser dehumidifier is located at the lower part of the back.

[0015] Furthermore, the ultrasonic humidifier is positioned at the center of the back of the incubator.

[0016] Furthermore, a temperature and humidity sensor group and a gas sensor group are provided on the top of the enclosure, and the temperature and humidity sensor group and the gas sensor group are electrically connected to the controller.

[0017] Furthermore, the conductive sheet is circumferentially attached to the outer wall of the incubator, and the conductive sheet is provided with a wavy heat dissipation pattern.

[0018] Furthermore, a thermally conductive silicone layer with a thickness of 0.3 to 0.5 mm is provided between the semiconductor cooling and heating module and the conductive plate to fill the gap between them.

[0019] Furthermore, an air replenishment device is provided on one side of the humidity-controlled air chamber, and the air replenishment device is connected to the air-controlled air chamber. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the structure of this utility model;

[0022] Figure 3 This is a schematic diagram of the structure of this utility model. Detailed Implementation

[0023] The reference numerals in the accompanying drawings include: 1. Base; 2. Chamber; 3. Incubator; 4. Conductive plate; 5. Circulation pipe; 6. Air filtration system; 7. Temperature and humidity sensor group; 8. Gas sensor group; 9. Camera; 10. Jet nozzle; 11. Pulse light generator; 12. Semiconductor cooling and heating module; 13. Ultrasonic humidifier; 14. Condensing dehumidifier; 15. Connecting pipe; 16. Controller; 17. Sealed door; 18. Movable door; 19. Air conditioning chamber; 20. Filter; 21. Nano-titanium dioxide photocatalytic coating; 22. Gas replenishment device.

[0024] Example 1 is basically as follows Figure 1-3 As shown, a biochemical incubator includes a base 1, a box body 2 is disposed on the base 1, an incubator 3 is fixedly installed inside the box body 2, and a gap is provided between the incubator 3 and the box body 2. The gap forms a heat insulation layer to reduce the interference of the external environment on the temperature inside the incubator 3.

[0025] A conductive sheet 4 is circumferentially attached to the outer wall of the incubator 3. The conductive sheet 4 is provided with a wavy heat dissipation pattern to increase the heat exchange area and improve the heat conduction efficiency. The semiconductor cooling and heating module 12 is fixedly connected to the conductive sheet 4. A thermally conductive silicone layer with a thickness of 0.3 to 0.5 mm is provided between the two to fill the gap between them and enhance the heat transfer effect. The semiconductor cooling and heating module 12 utilizes the Peltier effect of semiconductors to achieve precise temperature control of the incubator 3 through the conductive sheet 4, so that heat can be quickly and evenly conducted into the incubator 3.

[0026] The ultrasonic humidifier 13 is located at the center of the back of the incubator 3 and is connected to the incubator 3 through a pipe. It uses high-frequency oscillation to atomize water and delivers the water mist into the incubator 3 through the pipe to achieve efficient humidification and meet the humidity requirements of the sample.

[0027] A condenser dehumidifier 14 is installed at the lower back of the incubator 3. When the humidity inside the incubator 3 is too high, the hot and humid air will condense into water droplets when it encounters the surface of the condenser dehumidifier 14, which has a lower temperature. The water droplets will then flow downwards and be collected by gravity, thereby reducing the humidity inside the incubator 3 and maintaining humidity balance.

[0028] An air filtration system 6 is installed on one side of the chamber 2. This system includes a temperature and humidity control chamber 19 and a filter 20 installed vertically. Air passes through the filter 20 from bottom to top to remove impurities, microorganisms and other contaminants, and then enters the temperature and humidity control chamber 19 for precise temperature and humidity control. Afterward, the treated air is connected to the incubator 3 through the circulation pipe 5 to circulate and filter the air inside the chamber, maintaining a clean culture environment.

[0029] A gas replenishment device 22 is provided on one side of the humidity-controlled air chamber 19. The gas replenishment device 22 is connected to the air-controlled air chamber 19 and can replenish specific gases into the chamber according to the culture requirements, such as carbon dioxide gas in cell metabolism experiments, to ensure that the gas composition in the chamber meets the sample culture requirements.

[0030] The top two sides of the incubator 3 are equipped with pulsed light generators 11 at an angle. The angled design allows the pulsed light to provide instantaneous high-intensity sterilization of the interior of the incubator 3 with a wider coverage and more uniform irradiation, reducing sterilization blind spots caused by direct light exposure. This effectively kills potential bacteria, viruses and other microorganisms in every corner of the incubator. The inner wall of the incubator 3 is coated with a nano-titanium dioxide photocatalytic coating 21. Under the irradiation of the pulsed light generator 11, the coating is excited by photons to generate electron-hole pairs, which in turn generate hydroxyl radicals and superoxide anion radicals with strong oxidizing properties. These radicals can efficiently decompose residual organic pollutants, odor gases and toxins secreted by microorganisms in the incubator, achieving long-lasting sterilization, disinfection and self-cleaning effects.

[0031] A temperature and humidity sensor group 7 and a gas sensor group 8 are installed on the top of the incubator 3. They are electrically connected to a controller 16 located on the movable door 18 of the chamber 2. The temperature and humidity sensor group 7 monitors the temperature and humidity data inside the chamber in real time, and the gas sensor group 8 monitors the gas composition data inside the chamber, transmitting this data to the controller 16 in real time. The controller 16 integrates the control of modules such as temperature, humidity, and air circulation, automatically starting or stopping each component according to preset parameters to achieve intelligent adjustment. A sealing door 17 is movably installed on the incubator 3. The sealing door 17 fits tightly with the incubator 3 to achieve a sealed connection, preventing gas leakage inside the chamber and ensuring stable gas concentration. The sealing door is also hinged to the incubator for easy opening and closing. A movable door 18 is located on the front of the chamber 2, facilitating operation and observation of the interior of the incubator 3 by the operator.

[0032] In actual use, first check whether each component of the incubator 3 is working properly, including the operating status of the semiconductor cooling and heating module 12, ultrasonic humidifier 13, condenser dehumidifier 14, air filtration system 6, pulsed light generator 11, etc., and whether the data transmission of the temperature and humidity sensor group 7 and the gas sensor group 8 is normal.

[0033] Based on the requirements of the cultured samples, parameters such as temperature, humidity, and gas composition are preset on the controller 16. For example, for cell culture, the temperature is typically set to 37°C, the humidity to 95%, and the carbon dioxide concentration to 5%.

[0034] The inside of the incubator 3 is cleaned using cleaning tools, and then the pulsed light generator 11 is turned on for sterilization. At the same time, the nano-titanium dioxide photocatalytic coating 21 works synergistically under the pulsed light irradiation to further purify the internal environment of the incubator 3. The pulsed light generator 11 is tilted at 45°, with an irradiation intensity of 30000μWs / cm² and an irradiation time of 5-10 seconds, which can cover 99% of the space area inside the incubator 3. The nano-titanium dioxide photocatalytic coating 21 is applied by spraying process, with a coating thickness of 5-10μm. Under the pulsed light irradiation, it can generate hydroxyl radicals, and the degradation rate of formaldehyde and bacteria in the chamber exceeds 98% within 2 hours.

[0035] Open the movable door 18 and the sealing door 17, and carefully place the sample to be cultured in a suitable position inside the incubator 3. Avoid touching the incubator walls to prevent affecting the internal environment or damaging the sample. After placement, close the sealing door 17, ensuring it is tightly sealed to the incubator 3.

[0036] The semiconductor cooling and heating module 12 starts working based on the preset temperature and the data fed back by the temperature and humidity sensor group 7. When the temperature inside the chamber is higher than the set value, the semiconductor cooling and heating module 12 absorbs heat through the conductive plate 4 for cooling, and the conductive plate 4 with its wavy heat dissipation pattern accelerates heat dissipation; when the temperature is lower than the set value, the module generates heat and quickly transfers it to all parts of the incubator 3 through the conductive plate 4, achieving high-precision temperature control of ±0.1℃ and ensuring that the sample grows in a stable temperature environment.

[0037] The ultrasonic humidifier 13 operates based on data from the preset humidity and temperature / humidity sensor group 7. When the humidity is lower than the set value, the humidifier starts, atomizing water through high-frequency oscillation and delivering it into the incubator 3; when the humidity reaches or exceeds the set value, the humidifier stops working, stabilizing the humidity within the set value ±2%. Simultaneously, the condenser dehumidifier 14 monitors the humidity in real time, activating the dehumidification function when the humidity is too high to ensure humidity balance.

[0038] The air filtration system 6 operates continuously. After impurities and microorganisms are removed by the filter 20, the air enters the temperature and humidity regulating chamber 19 for temperature and humidity regulation, and then enters the incubator 3 through the circulation pipe 5, providing a clean and temperature- and humidity-appropriate air environment for the samples. The gas replenishment device 22 replenishes the required gas into the chamber in a timely manner according to the data monitored by the gas sensor group 8 and the preset gas composition parameters. When the air filtration system 6 is running, the air passes through the filter 20 (filtration accuracy 0.1μm) and the temperature and humidity regulating chamber 19 from bottom to top. The treated clean air passes through the circulation pipe 5, with the air inlet located at the top of the incubator 3 and the air outlet located at the bottom, forming vertical convection and circulating more than 20 times per hour.

[0039] During the cultivation process, the temperature and humidity sensor group 7 and the gas sensor group 8 monitor the environmental parameters inside the chamber in real time and transmit the data to the controller 16. The controller 16 analyzes and judges the data according to preset parameters and algorithms. If the monitored data deviates from the set range, it responds quickly and precisely adjusts the operating status of each component to ensure that the cultivation environment is always in a suitable state. For example, when the gas sensor group 8 detects that the carbon dioxide concentration is insufficient, the controller 16 immediately starts the gas replenishment device 22 to replenish carbon dioxide.

[0040] After incubation, open the sealed door 17 and the movable door 18, and carefully remove the sample. Shut down all operating modules, such as the semiconductor cooling and heating module 12, the ultrasonic humidifier 13, and the air filtration system 6. Clean and disinfect the incubator 3 again.

[0041] The above are merely embodiments of this utility model. Commonly known structures and characteristics are not described in detail here. Those skilled in the art are aware of all common technical knowledge in the field prior to the application date or priority date, are aware of all existing technologies in that field, and have the ability to apply conventional experimental methods prior to that date. Those skilled in the art can, based on the guidance provided in this application, improve and implement this solution in combination with their own capabilities. Some typical known structures or methods should not be obstacles for those skilled in the art to implement this application. It should be noted that those skilled in the art can make several modifications and improvements without departing from the structure of this utility model. These should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A biochemical incubator, characterized in that: The device includes a base, on which a housing is mounted. An incubator is fixedly mounted inside the housing, and a conductive plate is mounted on the incubator and fitted against it. A semiconductor cooling / heating module is mounted on the incubator and fixedly connected to the conductive plate. An air filtration system is mounted on one side of the housing and connected to the incubator via a circulation pipe. An ultrasonic humidifier is located below the semiconductor cooling / heating module and connected to the incubator via a pipe. A sealing door is movably mounted on the incubator and sealed to it. A movable door is mounted on the housing, and a controller is mounted on the movable door and fixedly mounted thereon. A gap is provided between the incubator and the housing. The controller is electrically connected to a temperature and humidity sensor group and a gas sensor group, and is used to automatically adjust the operating status of the semiconductor cooling / heating module, the ultrasonic humidifier, and the air filtration system based on real-time monitoring data.

2. A biochemical incubator according to claim 1, characterized in that, The air filtration system includes a temperature and humidity regulating chamber and a filter, which are installed vertically stacked.

3. A biochemical incubator according to claim 1, characterized in that, The top two sides of the enclosure are equipped with inclined pulsed light generators.

4. A biochemical incubator according to claim 1, characterized in that, The inner wall of the incubator is coated with a nano-titanium dioxide photocatalytic coating.

5. A biochemical incubator according to claim 1, characterized in that, A condenser dehumidifier is installed on the back of the incubator, and the condenser dehumidifier is located at the lower part of the back.

6. A biochemical incubator according to claim 1, characterized in that, The ultrasonic humidifier is located at the center of the back of the incubator.

7. A biochemical incubator according to claim 1, characterized in that, The top of the enclosure is equipped with a temperature and humidity sensor group and a gas sensor group, which are electrically connected to the controller.

8. A biochemical incubator according to claim 1, characterized in that, The conductive sheet is circumferentially attached to the outer wall of the incubator, and the conductive sheet is provided with a wavy heat dissipation pattern.

9. A biochemical incubator according to claim 1, characterized in that, A thermally conductive silicone layer with a thickness of 0.3 to 0.5 mm is provided between the semiconductor cooling and heating module and the conductive plate to fill the gap between them.

10. A biochemical incubator according to claim 2, characterized in that, An air replenishment device is provided on one side of the humidity-controlled air chamber, and the air replenishment device is connected to the air-controlled air chamber.

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