Multifunctional brain slice incubation bath

Abstract

The utility model relates to biomedical experiment equipment technical field discloses a kind of multifunctional brain slice constant-temperature incubation tank, including tank body, incubation assembly and control box.Tank body is double-layered hollow shell, filled with heat-conducting silica gel, bottom is provided with flexible electrothermal film, its surface etching spiral resistance grid, coverage area is 80% more than tank bottom area, downside has ceramic heat insulating sheet, cooperate multipoint temperature sensor and temperature controller using PID control algorithm, realize accurate temperature control.Incubation assembly contains incubation rack of being jointed in tank body, there are multiple rows of incubation tank on the frame, load net and bubble separation net are arranged in layers in tank, bottom corresponds bubble stone and flow guide cylinder.Gas distribution chamber is arranged in control box, connected with bubble stone through air distribution pipe and branch pipe, constitute gas supply system.The structure has excellent heat insulation performance and thermal stability, heating is uniform, can effectively protect brain slice activity, improve incubation effect, with significant technical advantage and practical value.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental equipment technology, specifically a multifunctional brain slice constant temperature incubation tank. Background Technology

[0002] In research fields such as neuroscience, brain slice incubation is a key experimental technique used to simulate the physiological environment of brain tissue in vitro and to conduct related biological research.

[0003] Traditional incubation devices typically employ simple heating elements, such as ordinary heating wires, along with a single temperature monitoring point for temperature regulation. This makes it difficult to achieve precise and uniform temperature control of the incubation environment. Since brain slices are extremely sensitive to temperature, even minor fluctuations in localized temperature can severely impact their activity, thereby interfering with the accuracy and reliability of experimental results. Furthermore, existing heating structures often suffer from uneven heat distribution, leading to significant temperature differences in different areas of the incubation tank. This fails to provide a stable and uniform temperature environment for the brain slices, which greatly limits the quality and efficiency of brain slice incubation experiments.

[0004] Meanwhile, the gas supply system of traditional incubation devices often releases bubbles directly into the culture medium through a single bubble stone. The bubble group comes into contact with the brain slices without being effectively dispersed, and the resulting fluid shear force can easily damage the brain slice structure. In addition, the oxygen dissolution efficiency is insufficient, making it difficult to meet the stable oxygen concentration requirements for long-term incubation.

[0005] Therefore, a multifunctional constant temperature incubator for brain slices is proposed to address the current shortcomings. Utility Model Content

[0006] To address the problems of existing technologies, this invention provides a multifunctional constant-temperature incubation tank for brain slices.

[0007] The technical problem to be solved by this utility model is to overcome the defects of the above-mentioned technology and provide a multifunctional brain slice constant temperature incubation tank.

[0008] To solve the above-mentioned technical problems, the technical solution provided by this utility model is a multifunctional brain slice constant temperature incubation tank, including a tank body, incubation components and a control box;

[0009] The tank has a double-layer hollow shell filled with thermally conductive silicone. An electrothermal film is provided in the thermally conductive silicone at the bottom of the shell, and a temperature sensor is provided at the bottom of the shell.

[0010] The incubation assembly includes an incubation rack that snaps into the shell, a gas distribution chamber, and bubble stones. The incubation rack is provided with multiple incubation tanks. The incubation tanks are provided with a material carrying net and a bubble-proof net in layers. The gas distribution chamber is located inside the control box. The shell is provided with a gas distribution pipe connected to the gas distribution chamber. Bubble stones connected to the gas distribution pipe are provided below the incubation tanks.

[0011] The control box is equipped with a heater connected to an electric heating film, and a temperature controller connected to the heater and a temperature sensor is located on the front side of the control box.

[0012] As an improvement, the electrothermal film is a flexible electrothermal film, and a spiral resistor grid is etched on the surface of the electrothermal film. The coverage area of ​​the electrothermal film accounts for more than 80% of the bottom area of ​​the tank.

[0013] As an improvement, a ceramic heat insulation sheet is provided on the lower side of the electrothermal film to contact the housing.

[0014] As an improvement, the number of temperature sensors is multiple, and the multiple temperature sensors are respectively set at the four corners and the middle of the inner surface of the bottom of the tank.

[0015] As an improvement, the tank is provided with snap-fit ​​components at both ends, the incubation rack is adapted to the tank and has snap-fit ​​slots at both ends that cooperate with the snap-fit ​​components, and there is a certain distance between the bottom of the incubation rack and the tank.

[0016] As an improvement, multiple incubation tanks are arranged in rows along the length of the incubation rack, and the incubation tanks have a hollow cylindrical structure.

[0017] As an improvement, the bubble-sparing mesh is located at the bottom of the incubation tank, and the material-carrying mesh is located in the middle of the incubation tank and above the bubble-sparing mesh, and the pore size of the bubble-sparing mesh is 50μm.

[0018] As an improvement, the gas distribution chamber is fixedly installed in the upper part of the control box, and the gas distribution chamber is provided with an air inlet pipe extending to the outside of the control box, and a flow regulating valve is provided on the air inlet pipe.

[0019] As an improvement, the gas distribution pipe is arranged along the length of the bottom of the tank, with one end extending upward and passing through the tank wall to communicate with the gas distribution chamber.

[0020] As an improvement, the air distribution pipe is connected to the air bubble stone through a branch pipe, and the bottom of the incubation rack is provided with a guide tube that communicates with the bottom of the incubation tank. The upper part of the guide tube is provided with multiple air holes in the circumferential direction, and the air bubble stone is located inside the guide tube.

[0021] The advantages of this utility model compared with the prior art are as follows:

[0022] 1. A double-layered hollow shell structure is adopted, with thermally conductive silicone filling the interior to form a highly efficient insulation layer and heat transfer medium. This significantly improves the tank's insulation performance and ensures rapid and uniform heat transfer throughout the tank, avoiding uneven temperature distribution. Furthermore, multiple temperature sensors at the bottom of the tank monitor the temperature in different areas in real time. A PID control algorithm, used by a temperature controller, dynamically adjusts the heater output power, achieving precise temperature control throughout the tank. This effectively solves the temperature fluctuation problem that may exist in existing technologies, ensuring that brain slices are incubated in a stable temperature environment.

[0023] 2. The spiral resistor grid etched on the surface of the heating film increases the resistance path length and surface area, thereby improving thermal efficiency. Simultaneously, the heating film covers more than 80% of the tank bottom area, ensuring comprehensive and uniform heating. This not only improves heating efficiency but also avoids localized overheating or underheating, providing a uniform thermal environment for brain slice incubation.

[0024] 3. By employing components such as a gas distribution chamber, gas distribution pipe, and air stone, and through the combination of a flow guide tube and vent holes, uniform diffusion of bubbles and circulation of gas are achieved, effectively increasing the dissolved oxygen content in the culture medium and reducing the damage to brain slices caused by fluid shear force, thus providing a favorable microenvironment for brain slice incubation.

[0025] 4. The incubation rack is connected to the tank via snap-fit ​​connectors, achieving a suspended installation that avoids direct contact with the tank and prevents sample overheating. Simultaneously, the snap-fit ​​design of the incubation rack makes installation and disassembly more convenient, facilitating experimental operation and equipment maintenance. This not only improves the practicality of the equipment but also ensures the stable installation of the incubation rack within the tank, providing stable support for the incubation of brain slices.

[0026] 5. The layered material carrier net and bubble-proof net design in the incubation tank effectively supports the brain slice specimen and prevents air bubbles from directly contacting the sample, reducing the damage to the brain slice caused by fluid shear force. The double-layer design protects the brain slice structure while ensuring full contact between the solution and the brain slice, thus improving the incubation effect. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the structure of a multifunctional brain slice constant temperature incubation tank according to this utility model.

[0028] Figure 2 This is a schematic diagram of the internal structure of a multifunctional brain slice constant temperature incubator according to this utility model. Figure 1 .

[0029] Figure 3 yes Figure 2 A magnified view of a portion of point A in the middle.

[0030] Figure 4 This is a schematic diagram of the internal structure of a multifunctional brain slice constant temperature incubator according to this utility model. Figure 2 .

[0031] Figure 5 This is a schematic diagram of the internal structure of the control box in a multifunctional brain slice constant temperature incubation tank according to this utility model.

[0032] Figure 6 This is a top view of a multifunctional brain slice constant temperature incubator according to this utility model. Figure 1 .

[0033] Figure 7 yes Figure 6 Sectional view at point BB.

[0034] Figure 8 yes Figure 7 A magnified view of a section at point C.

[0035] Figure 9 This is a top view of a multifunctional brain slice constant temperature incubator according to this utility model. Figure 2 .

[0036] Figure 10 yes Figure 9 Sectional view at point DD.

[0037] As shown in the figure:

[0038] 1. Tank body; 101. Shell;

[0039] 2. Incubation components; 201. Incubation rack; 202. Gas distribution chamber; 203. Air stone; 204. Incubation tank; 205. Material carrier net; 206. Bubble separator net; 207. Gas distribution pipe; 208. Connecting piece; 209. Slot; 2010. Air inlet pipe; 2011. Flow regulating valve; 2012. Branch pipe;

[0040] 3. Control box; 4. Thermally conductive silicone; 5. Heating film; 6. Temperature sensor; 7. Heater; 8. Thermostat; 9. Ceramic heat insulation sheet; 10. Flow guide tube; 11. Vent. Detailed Implementation

[0041] To make the objectives, technical solutions, and advantages of the utility model embodiments clearer, the technical solutions of the utility model embodiments will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the utility model, not all embodiments. The components of the utility model embodiments described and shown in the accompanying drawings can typically be arranged and designed in various different configurations.

[0042] In the description of the embodiments of the utility model, it should be noted that if terms such as "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," or "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use, they are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the utility model. Furthermore, terms such as "first," "second," and "third" are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0043] Furthermore, the use of terms such as "horizontal," "vertical," and "sag" does not imply that the component must be absolutely horizontal or suspended, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0044] In the description of the utility model embodiments, "a plurality of" means at least two.

[0045] In the description of the embodiments of the utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in the utility model according to the specific circumstances.

[0046] As shown in the attached figures, a multifunctional brain slice constant temperature incubation tank includes a tank body 1, an incubation component 2, and a control box 3.

[0047] The tank 1 has a double-layer hollow shell 101. The hollow double-layer structure of the tank 1 forms a heat insulation layer, which has significant heat insulation performance and thermal stability. It is filled with thermally conductive silicone 4. The thermally conductive silicone 4 forms a uniform heat conduction medium in the shell 101. Due to the good thermal conductivity of the thermally conductive silicone 4, it can quickly and evenly transfer heat to the entire tank 1, and at the same time make the heat distribution in the tank 1 more uniform.

[0048] An electrothermal film 5 is provided in the thermally conductive silicone 4 at the bottom of the housing 101. The electrothermal film 5 is a flexible electrothermal film that can adapt to the deformation of the housing 101. A spiral resistor grid is etched on the surface of the electrothermal film 5. The coverage area of ​​the electrothermal film 5 accounts for more than 80% of the bottom area of ​​the tank 1.

[0049] In practice, the spiral resistor grid design etched on the surface of the heating film 5 increases the resistance path length and surface area, thereby improving thermal efficiency. Simultaneously, the heating film 5 covers more than 80% of the tank bottom area, ensuring comprehensive and uniform heating.

[0050] Furthermore, the lower side of the electric heating film 5 is provided with a ceramic heat insulation sheet 9 that contacts the housing 101, which can prevent heat from being lost downwards and enhance the heat preservation performance.

[0051] The bottom of the housing 101 is equipped with a temperature sensor 6, which can monitor the temperature in different areas of the tank 1 in real time, forming multi-point temperature monitoring, comprehensively grasping the temperature distribution in the tank 1, and avoiding the impact of local temperature fluctuations on brain slice activity.

[0052] There are multiple temperature sensors 6, which are respectively set at the four corners and the middle of the inner surface of the bottom of the tank 1. The temperature sensors 6 monitor the temperature in different areas of the tank 1 in real time.

[0053] The incubation assembly 2 includes an incubation rack 201 that snaps into the housing 101, a gas distribution chamber 202, and an air stone 203. The tank 1 has snap-fit ​​parts 208 at both ends. The incubation rack 201 is adapted to the tank 1 and has slots 209 at both ends that cooperate with the snap-fit ​​parts 208. The incubation rack 201 is suspended in the tank 1 through the cooperation of the snap-fit ​​parts 208 and the slots 209, avoiding direct contact with the tank 1 and preventing the sample from overheating. At the same time, it can ensure the stable installation of the incubation rack 201 on the tank 1 and facilitate the quick assembly and disassembly of the incubation rack 201 on the tank 1, which is convenient for experimental operation and equipment maintenance.

[0054] A certain distance is provided between the bottom of the incubation rack 201 and the tank 1 to provide installation space for the gas distribution pipe 207 and the air stone 203, and at the same time, to provide space for gas circulation.

[0055] The incubation rack 201 is provided with multiple incubation slots 204 for holding brain slices. Specifically, the multiple incubation slots 204 are arranged in a row along the length of the incubation rack 201. The incubation slots 204 have a hollow cylindrical structure. By setting up multiple incubation slots 204, multiple brain slices can be incubated.

[0056] The incubation tank 204 is equipped with a layered material carrier net 205 and a bubble-sparing net 206. The material carrier net 205 supports the brain slice specimen; the bubble-sparing net 206 prevents air bubbles from directly contacting the sample, reducing damage to the brain slices caused by fluid shear forces. The double-layer design of the material carrier net 205 and the bubble-sparing net 206 effectively protects the brain slice structure while ensuring sufficient contact between the solution and the brain slice.

[0057] In this embodiment, the bubble-sparing mesh 206 is located at the bottom of the incubation tank 204, and the material carrier mesh 205 is located in the middle of the incubation tank 204 and above the bubble-sparing mesh 206. In this embodiment, the pore size of the bubble-sparing mesh 206 is 50μm.

[0058] The gas distribution chamber 202 is located inside the control box 3. The gas distribution chamber 202 is fixedly located in the upper part of the control box 3. The gas distribution chamber 202 is provided with an air inlet pipe 2010 extending to the outside of the control box 3. The air inlet pipe 2010 is connected to an external gas source. The air inlet pipe 2010 is provided with a flow regulating valve 2011. The air flow rate is adjusted by adjusting the opening degree of the flow regulating valve 2011.

[0059] The housing 101 is provided with a gas distribution pipe 207 that connects to the gas distribution chamber 202. The gas distribution pipe 207 is arranged along the length of the bottom of the tank 1, with one end extending upward and passing through the tank wall of the tank 1 to communicate with the gas distribution chamber 202, so as to ensure that the gas can be evenly distributed to the bottom of each incubation tank 204.

[0060] Below the incubation tank 204, there is a bubble stone 203 connected to the gas distribution pipe 207. The gas distribution pipe 207 is connected to the bubble stone 203 through a branch pipe 2012. The branch pipe 2012 delivers the gas from the gas distribution pipe 207 to each bubble stone 203, forming an independent gas path.

[0061] Furthermore, the bottom of the incubation rack 201 is provided with a guide tube 10 that communicates with the bottom of the incubation tank 204. The guide tube 10 has a hollow cylindrical structure and is coaxially arranged with the incubation tank 204. The upper part of the guide tube 10 is provided with multiple ventilation holes 11. The air stone 203 is located inside the guide tube 10. Specifically, the air stone 203 is located below the ventilation holes 11.

[0062] Specifically, the air stone 203 is located in the lower part of the guide tube 10. The guide tube 10 guides the air bubbles to diffuse evenly to the bubble-blocking net 206. At the same time, the bubble-blocking net 206 blocks the air bubble group and diffuses it to the outside of the guide tube 10 through the vent 11.

[0063] The control box 3 is equipped with a heater 7 connected to the electric heating film 5. The heater 7 supplies power to the electric heating film 5. The front of the control box 3 is equipped with a temperature controller 8 connected to the heater 7 and the temperature sensor 6. The temperature controller 8 receives the temperature signal from the temperature sensor 6 and controls the heater 7 to dynamically adjust the output power.

[0064] The temperature controller 8 adopts a PID control algorithm, which can dynamically adjust the power output of the heater 7 according to the deviation between the actual temperature fed back by the temperature sensor 6 and the set value, so as to achieve precise control of the overall temperature in the tank 1.

[0065] In this embodiment, the temperature controller 8 is a Shimaden SR23 digital controller, the temperature sensor 6 is a Heraeus M222 series PT100 platinum resistance thermometer, and the heating film 5 is a Minco HK9100 series flexible silicone-based etched foil heating film.

[0066] In specific implementation of this utility model:

[0067] 1. Preparation stage:

[0068] 1.1 Install the incubation rack: Align and snap the incubation rack 201 with the snap-fit ​​parts 208 on the side wall of the tank 1 through the snap-fit ​​grooves 209 at both ends, so that the incubation rack 201 is suspended and fixed in the tank 1.

[0069] 1.2 Placing brain slices: Place the brain slice specimen on the material carrier net 205 in the middle of the incubation tank 204, ensuring that the bubble-proof net 206 is below the material carrier net 205.

[0070] 1.3 Connect the gas source: Connect the external gas source pipeline to the air inlet pipe 2010 of the gas distribution chamber 202, and adjust the flow regulating valve 2011 on the air inlet pipe 2010 to the preset ventilation speed.

[0071] 1.4 Setting parameters: Set the target temperature of tank 1 (e.g., 32℃±0.5℃) through the temperature controller 8 on the front side of control box 3.

[0072] 2) Heating and temperature control stage:

[0073] 2.1 Heating Start-up: Thermostat 8 controls heater 7 to supply power to electric heating film 5, and electric heating film 5 generates heat efficiently through surface spiral resistive grid.

[0074] 2.2 Heat transfer process: Due to the good thermal conductivity of the thermally conductive silicone 4, heat is evenly diffused to the entire area of ​​the tank 1 through the thermally conductive silicone 4, while the ceramic heat insulation sheet 9 prevents heat from being lost downwards.

[0075] 2.3 Dynamic temperature control: Multiple temperature sensors 6 at the four corners and the middle of the tank 1 monitor the temperature of different areas inside the tank 1 and transmit the temperature signals to the temperature controller 8. The temperature controller 8 adopts a PID control algorithm and dynamically adjusts the output power of the heater 7 according to the deviation between the received actual temperature and the set temperature to achieve precise control of the overall temperature inside the tank 1 and ensure that the brain slices are in a stable temperature environment.

[0076] 3. Gas supply and microenvironment construction stage:

[0077] 3.1 Gas delivery: External gas source gas enters the gas distribution chamber 202 through the gas inlet pipe 2010, and is delivered to each air bubble stone 203 through the gas distribution pipe 207 and the branch pipe 2012.

[0078] 3.2 Bubble generation and diffusion: The bubble stone 203 generates a group of microbubbles in the guide tube 10. The bubble group diffuses upward through the guide tube 10 and is blocked and dispersed by the bubble barrier 206, which slows down the rising speed. The dispersed gas bypasses the bubble barrier 206 and enters the culture medium in the incubation tank 204, which increases the dissolved oxygen and reduces the fluid shear force.

[0079] 3.3 Gas circulation: After the bubble group is blocked by the bubble barrier 206, some of the bubble group diffuses through the vent 11 on the guide tube 10 to the space between the bottom of the tank 1 and the incubation rack 201 to form a circulating airflow and ensure uniform oxygen distribution.

[0080] Incubation Stage: Under the set temperature and gas environment, the brain slices are incubated in incubation tank 204. During incubation, the incubation effect can be evaluated at any time by observing the state of the brain slices and detecting relevant indicators in the solution. If it is necessary to adjust the temperature or gas supply parameters, it can be adjusted accordingly through temperature controller 8 or flow regulating valve 2011.

[0081] 4. Monitoring and Adjustment Stage During Incubation:

[0082] 4.1 Environmental Maintenance: The thermostat 8 continuously and dynamically adjusts the power of the heating film 5 to maintain temperature stability; the flow regulating valve 2011 maintains a constant ventilation rate.

[0083] 4.2 Status monitoring: The morphological integrity of the brain slices on the carrier mesh 205 was observed under a microscope; the pH value and dissolved oxygen content of the solution were detected to assess the metabolic environment.

[0084] 5. End Stage: After incubation, turn off heater 7 to stop power supply to heating film 5, and shut off external gas source to cut off gas supply. Remove brain slices for subsequent experimental operations or preservation. Simultaneously, clean and maintain components such as incubation tank 204 and incubation rack 201 for future use.

[0085] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.

Claims

1. A multifunctional brain slice constant temperature incubator, characterized in that: It includes a tank (1), an incubation assembly (2), and a control box (3); The tank (1) has a double-layer hollow shell (101) filled with thermally conductive silicone (4). An electrothermal film (5) is provided in the thermally conductive silicone (4) at the bottom of the shell (101). A temperature sensor (6) is provided at the bottom of the shell (101). The incubation assembly (2) includes an incubation rack (201) that is snapped into the housing (101), a gas distribution chamber (202), and an air stone (203). The incubation rack (201) is provided with multiple incubation tanks (204). The incubation tanks (204) are provided with a material carrier net (205) and a bubble separator net (206) in layers. The gas distribution chamber (202) is located in the control box (3). The housing (101) is provided with a gas distribution pipe (207) that connects to the gas distribution chamber (202). The air stone (203) that connects to the gas distribution pipe (207) is provided below the incubation tanks (204). The control box (3) is equipped with a heater (7) connected to the electric heating film (5), and a temperature controller (8) connected to the heater (7) and the temperature sensor (6) is provided on the front side of the control box (3).

2. The multifunctional brain slice constant temperature incubator according to claim 1, characterized in that: The electrothermal film (5) is a flexible electrothermal film, and a spiral resistor grid is etched on the surface of the electrothermal film (5). The coverage area of ​​the electrothermal film (5) accounts for more than 80% of the bottom area of ​​the tank (1).

3. The multifunctional brain slice constant temperature incubator according to claim 2, characterized in that: The lower side of the electric heating film (5) is provided with a ceramic heat insulation sheet (9) that contacts the shell (101).

4. The multifunctional brain slice constant temperature incubator according to claim 1, characterized in that: The number of temperature sensors (6) is multiple, and the multiple temperature sensors (6) are respectively set at the four corners and the middle of the inner surface of the bottom of the tank (1).

5. The multifunctional brain slice constant temperature incubator according to claim 1, characterized in that: The tank (1) has snap-fit ​​parts (208) at both ends. The incubation rack (201) is adapted to the tank (1) and has slots (209) at both ends that cooperate with the snap-fit ​​parts (208). There is a certain distance between the bottom of the incubation rack (201) and the tank (1).

6. The multifunctional brain slice constant temperature incubator according to claim 5, characterized in that: Multiple incubation tanks (204) are arranged in a row along the length of the incubation rack (201), and the incubation tanks (204) have a hollow cylindrical structure.

7. A multifunctional brain slice constant temperature incubator according to claim 6, characterized in that: The bubble-sparing mesh (206) is located at the bottom of the incubation tank (204), and the material carrier mesh (205) is located in the middle of the incubation tank (204) and above the bubble-sparing mesh (206). The pore size of the bubble-sparing mesh (206) is 50 μm.

8. The multifunctional brain slice constant temperature incubator according to claim 1, characterized in that: The gas distribution chamber (202) is fixedly installed in the upper part of the control box (3). The gas distribution chamber (202) is provided with an air inlet pipe (2010) extending to the outside of the control box (3). The air inlet pipe (2010) is provided with a flow regulating valve (2011).

9. A multifunctional brain slice constant temperature incubator according to claim 8, characterized in that: The gas distribution pipe (207) is arranged along the length of the bottom of the tank (1), with one end extending upward and passing through the tank wall of the tank (1) to communicate with the gas distribution chamber (202).

10. A multifunctional brain slice constant temperature incubator according to claim 9, characterized in that: The air distribution pipe (207) is connected to the air bubble stone (203) through the branch pipe (2012). The bottom of the incubation rack (201) is provided with a guide tube (10) that communicates with the bottom of the incubation tank (204). The upper part of the guide tube (10) is provided with multiple air holes (11) in the circumferential direction. The air bubble stone (203) is located inside the guide tube (10).

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