A multimodal microscopic spectral imaging integrated detection system

Abstract

This invention provides a multimodal microscopic imaging integrated detection system, relating to the field of microscopic optical detection technology. It includes a bright-field microscopic imaging module sharing a tissue section sampling optical path assembly, and at least two of three modules: a polarized light microscopic imaging module, a fluorescence microscopic imaging module, and a micro-Raman spectroscopy detection module. The bright-field, polarized light, and fluorescence microscopic imaging modules share an imaging optical path assembly. The micro-Raman spectroscopy detection module includes a Raman spectrometer and a laser for irradiating tissue sections to excite Raman scattering light. The beneficial effect of this invention is that, based on bright-field microscopic imaging, it introduces multiple characteristic imaging or spectroscopic technologies such as polarized light imaging, fluorescence imaging, and Raman spectroscopy. Through multimodal information complementarity, it enriches the differential information between cancerous tissues and normal, inflammatory lesions, etc., significantly improving the speed and efficiency of pathological examination and enabling rapid and accurate intraoperative cancer diagnosis.

Description

Technical Field

[0001] This utility model relates to the field of microscopic optical detection technology, and in particular to a multimodal microscopic spectral imaging integrated detection system. Background Technology

[0002] Bright-field microscopy (conventional optical microscopy) has been widely used to observe the microscopic tissue structure and cellular characteristics of biological tissue sections for pathological examination, and is the "gold standard" for cancer diagnosis.

[0003] However, it usually requires complex and time-consuming hematoxylin and eosin (H&E) staining to highlight cell morphology, number and distribution.

[0004] The applicant has discovered that the prior art has at least the following technical problems:

[0005] Eliminating H&E staining can significantly improve the speed of pathological examination and enable rapid intraoperative cancer diagnosis. However, the loss of cellular characteristics will directly reduce the accuracy of cancer diagnosis, requiring the addition of other microscopic feature information to maintain accurate cancer diagnosis.

[0006] Therefore, there is an urgent need for a multimodal microscopic spectral imaging integrated detection system to solve the above-mentioned technical problems. Utility Model Content

[0007] The purpose of this invention is to provide a multimodal microscopic imaging integrated detection system. Based on bright-field microscopy, it introduces various characteristic imaging or spectroscopic techniques such as polarized light imaging, fluorescence imaging, and Raman spectroscopy. Polarized light imaging can extract anisotropic microstructures and distribution characteristics, such as collagen fibers; fluorescence imaging can extract the distribution characteristics of fluorescent molecules, such as collagen and FAD; and Raman spectroscopy can extract tissue molecular composition characteristics with high specificity. The complementary multimodal information enriches the differential information between cancerous, normal, and inflammatory tissues. The numerous technical effects of the preferred solutions provided by this invention are detailed below.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] This utility model provides a multimodal microscopic imaging integrated detection system, including a bright-field microscopic imaging module that shares a tissue section sampling optical path component, and at least two of a polarized light microscopic imaging module, a fluorescence microscopic imaging module, and a micro-Raman spectroscopy detection module. The bright-field microscopic imaging module, the polarized light microscopic imaging module, and the fluorescence microscopic imaging module share an imaging optical path component. The micro-Raman spectroscopy detection module includes a Raman spectrometer and a laser for emitting Raman light onto the tissue section.

[0010] Preferably, the sampling optical path assembly includes:

[0011] The components arranged from bottom to top along the first axis are: a first light source, a condenser lens, a sampling assembly, a quarter lens box, and a half lens box; and

[0012] A second light source, a converging lens group, and a laser are arranged along a second axis perpendicular to the first axis, with the laser and the converging lens group respectively disposed on both sides of the quarter-lens box.

[0013] Preferably, the sampling optical path assembly further includes:

[0014] The first polarization component is coaxially disposed between the first light source and the condenser lens;

[0015] A polarization analyzer is coaxially disposed between the split lens box and the quarter lens box.

[0016] Preferably, the sampling optical path assembly further includes:

[0017] The second polarization assembly is coaxially disposed between the converging lens group and the quarter lens box.

[0018] Preferably, the quarter-lens box includes a semi-transparent and semi-reflective mirror box unit, a fluorescence dichroic mirror box unit, a Raman dichroic mirror box unit, and a first empty box unit, wherein:

[0019] The semi-transparent and semi-reflective mirrors in the semi-transparent and semi-reflective mirror box unit and the fluorescent dichroic mirrors in the fluorescent dichroic mirror box unit are arranged in the same direction;

[0020] The Raman dichroic mirror in the Raman dichroic mirror box unit is set to form a 90° angle with the semi-transparent and semi-reflective mirror and the fluorescent dichroic mirror;

[0021] A filter is provided inside the fluorescent dichroic mirror box unit.

[0022] Preferably, the sampling component includes:

[0023] Objective lens assembly;

[0024] The stage, located below the objective lens assembly, includes a stage body capable of moving along the XY axis and a lifting mechanism connected to the stage body for enabling the stage to move along the Z axis.

[0025] A tablet compression device is mounted on the loading platform.

[0026] Preferably, the imaging optical path assembly includes: an imaging lens group and an image sensor arranged coaxially, wherein the imaging lens group is disposed between the image sensor and the bipartite box.

[0027] Preferably, the micro Raman spectroscopy detection module further includes an optical fiber translation adjustment frame, an optical fiber coupler, and a positioning LED light source, which are sequentially arranged above the split-lens box. The optical fiber coupler includes a main body and an optical fiber bundle connected to the main body. The main body is coaxially arranged with the optical fiber translation adjustment frame and the positioning LED light source. The optical fiber bundle is arranged between the main body and the positioning LED light source.

[0028] Preferably, the fiber bundle is a Y-type fiber bundle, comprising:

[0029] A Raman collecting fiber is connected to the Raman spectrometer;

[0030] The positioning light fiber is arranged in a "Y" shape with the Raman collecting fiber and connected to the positioning LED light source. The positioning light fiber is arranged in a ring around the Raman collecting fiber, and the cross-sectional area of ​​the positioning light fiber is smaller than that of the Raman collecting fiber.

[0031] Preferably, the split-lens box includes a reflector box unit and a second empty box unit.

[0032] The multimodal microscopic imaging integrated detection system provided by this utility model, by setting up at least two of the following three modules—a bright-field microscopic imaging module, a polarized light microscopic imaging module, a fluorescence microscopic imaging module, and a micro-Raman spectroscopy detection module—which share a common tissue slice sampling optical path component, can integrate bright-field imaging and at least two of polarized light imaging, fluorescence imaging, and Raman spectroscopy. It can perform spectral detection on the same area of ​​biological tissue slices. Based on bright-field microscopic imaging, polarized light imaging can extract anisotropic microstructure and distribution characteristics, such as collagen fibers; fluorescence imaging can extract the distribution characteristics of fluorescent molecules, such as collagen and FAD; and Raman spectroscopy can extract tissue molecular composition characteristics with high specificity. The multimodal information is complementary, enriching the differential information between cancerous tissues and normal, inflammatory lesions, etc., which can significantly improve the speed of pathological examination and diagnostic efficiency, and enable rapid and accurate intraoperative cancer diagnosis. Attached Figure Description

[0033] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0034] Figure 1 This is a schematic diagram of the structure of an embodiment of the multimodal microscopic spectral imaging integrated detection system of this utility model;

[0035] Figure 2 This is a schematic diagram of the bipartite box structure in the multimodal microscopic spectral imaging integrated detection system of this utility model;

[0036] Figure 3 This is a schematic diagram of the structure of the quarter-scope box in the multimodal microscopic spectral imaging integrated detection system of this utility model;

[0037] Figure 4 yes Figure 3 A structural diagram from another angle;

[0038] Figure 5 This is the bright-field transmission imaging optical path in the multimodal microscopic spectral imaging integrated detection system of this utility model;

[0039] Figure 6 This is the excitation optical path for fluorescence reflection imaging in the multimodal microscopic spectral imaging integrated detection system of this utility model;

[0040] Figure 7 This is the collection optical path for fluorescence reflection imaging in the multimodal microscopic spectral imaging integrated detection system of this utility model.

[0041] Figure 8 This is a schematic cross-sectional view of the fiber optic coupler in the multimodal microscopic spectral imaging integrated detection system of this utility model;

[0042] Figure 9 This is the Raman backscattering optical path in the multimodal microscopic spectral imaging integrated detection system of this utility model.

[0043] In the diagram: 1. First light source; 2. Polarizer assembly of the first polarizer; 3. Quarter-wave plate assembly of the first polarizer; 4. Condenser; 5. Lifting mechanism; 6. Stage; 7. Tablet pressing device; 8. Objective lens assembly; 9. Quarter lens box; 91. Semi-transparent mirror box unit; 92. Fluorescent dichroic mirror box unit; 93. Raman dichroic mirror box unit; 94. First empty box unit; 95. Filter; 10. Quarter-wave plate assembly of the analyzer; 11. Polarizer assembly of the analyzer; 12. 121. Dividing mirror box; 122. Reflecting mirror box unit; 123. Second empty box unit; 14. Imaging mirror group; 15. Image sensor; 16. Second light source; 17. Converging lens group; 18. Polarizer assembly of the second polarizing component; 19. Quarter wave plate assembly of the second polarizing component; 20. Laser; 21. Fiber optic translation adjustment frame; 22. Fiber optic coupler; 22. Fiber bundle; 221. Raman collection fiber; 222. Positioning light fiber; 23. Positioning LED light source; 24. Raman spectrometer. Detailed Implementation

[0044] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other implementation methods obtained by those skilled in the art without creative effort, as well as implementation methods that combine some spectral modes from this utility model, are within the scope of protection of this utility model.

[0045] Figure 1 This is a structural schematic diagram of this embodiment, as shown below. Figure 1 As shown, this utility model provides a multimodal microscopic imaging integrated detection system, including a bright-field microscopic imaging module that shares a tissue section sampling optical path component, and at least two of the following three modules: a polarized light microscopic imaging module, a fluorescence microscopic imaging module, and a micro-Raman spectroscopy detection module. The bright-field microscopic imaging module, the polarized light microscopic imaging module, and the fluorescence microscopic imaging module share an imaging optical path component. The micro-Raman spectroscopy detection module includes a Raman spectrometer 24 and a laser 19 for irradiating the tissue section to excite Raman scattering light.

[0046] This multimodal microscopic imaging integrated detection system incorporates at least two of the following: bright-field microscopy, polarized light microscopy, fluorescence microscopy, and micro-Raman spectroscopy. Multiple modules share a common tissue section sampling optical path, enabling spectral detection of the same region of biological tissue sections. By integrating bright-field imaging, polarized light imaging, fluorescence imaging, and Raman spectroscopy, and building upon bright-field microscopy, polarized light imaging can extract anisotropic microstructures and distribution characteristics, such as collagen fibers. Fluorescence imaging can extract the distribution characteristics of fluorescent molecules, such as collagen and FAD. Raman spectroscopy can extract tissue molecular composition characteristics with high specificity. The complementary multimodal information enriches the differential information between cancerous, normal, and inflammatory tissues, significantly improving the speed and efficiency of pathological examination and enabling rapid and accurate intraoperative cancer diagnosis.

[0047] As an optional implementation method, such as Figure 1 As shown, the sampling optical path assembly in this embodiment includes a first light source 1, a condenser lens 4, a sampling component, a quarter lens box 9, and a half lens box 12 arranged sequentially from bottom to top along the first axis. It also includes a second light source 15, a converging lens group 16, and a laser 19 arranged along a second axis perpendicular to the first axis. The laser 19 and the converging lens group 16 are respectively disposed on both sides of the quarter lens box 9.

[0048] The sampling assembly in this embodiment includes an objective lens assembly 8, a stage 6, a lifting mechanism 5, and a tablet pressing device 7. The stage 6 is located below the objective lens assembly 8 and includes a stage body that can move along the XY axis and a lifting mechanism 5 connected to the stage body to enable the stage to move along the Z axis.

[0049] To facilitate operation and allow for adjustment of the stage 6 according to actual needs, this embodiment provides a stage 6 comprising upper and lower layers. The lower layer is fixedly connected to the lifting mechanism 5, enabling vertical displacement of the stage 6 along the Z-axis through changes in its position relative to the lifting mechanism 5. A slide rail is provided between the upper and lower layers to allow horizontal displacement of the upper layer along the XY-axis. A tablet pressing device 7 is located on the upper layer of the stage 6. In this embodiment, the tablet pressing device 7 is a tablet clamp used to limit the tissue slices.

[0050] like Figure 2 As shown, the bipartite box 12 in this embodiment includes a reflecting mirror box unit 121 and a second empty box unit 122. The bipartite box 12 is switched by a push-pull method to correspond to the imaging and spectral modules respectively. The imaging optical path components in this embodiment include: an imaging mirror group 13 and an image sensor 14 arranged coaxially, with the imaging mirror group 13 disposed between the image sensor 14 and the bipartite box 12.

[0051] like Figure 3 and Figure 4 As shown, the quadrature box 9 includes a semi-transparent and semi-reflective mirror box unit 91, a fluorescence dichroic mirror box unit 92, a Raman dichroic mirror box unit 93, and a first empty box unit 94. The quadrature box 9 is switched by a push-pull method to correspond to different imaging / spectral modules.

[0052] The semi-transparent and semi-reflective mirror in the semi-transparent and semi-reflective mirror box unit 91 and the fluorescent dichroic mirror in the fluorescent dichroic mirror box unit 92 are arranged in the same direction; the Raman dichroic mirror in the Raman dichroic mirror box unit 93 is set to form a 90° angle with the semi-transparent and semi-reflective mirror and the fluorescent dichroic mirror; a filter 95 is provided on the surface of the fluorescent dichroic mirror box unit 92 facing the second light source 15, and the filter 95 can be added or removed from one side of the fluorescent dichroic mirror box unit 92 according to actual use needs.

[0053] During operation, the sampling optical path components can be used to form a transmission sampling optical path component for transmission imaging, a reflection sampling optical path component for reflection imaging, and a scattering sampling optical path component for scattering spectrum detection.

[0054] Specifically, during transmission imaging, the transmission sampling optical path assembly includes a first light source 1, a condenser lens 4, a sampling component, a quarter lens box 9, and a half lens box 12, which are arranged coaxially from bottom to top.

[0055] During reflection imaging, the reflection sampling optical path assembly includes a second light source 15, a converging lens group 16, a quarter-lens box 9, a sampling component, and a bipartite box 12.

[0056] During scattering spectroscopy detection, the scattering sampling optical path assembly includes a laser 19, a quarter-lens box 9, a sampling component, and a bi-lens box 12.

[0057] The bright-field microscopy module in this embodiment includes two forms: transmission imaging and reflection imaging.

[0058] like Figure 5 As shown, during transmission imaging, the optical path follows the transmission sampling optical path assembly, which includes a first light source 1, a condenser lens 4, a sampling assembly, a reflector in the reflector box unit 121, an imaging lens group 13, and an image sensor 14.

[0059] During reflection imaging, the optical path is sampled along the reflection optical path assembly, which includes a second light source 15, a converging lens group 16 and a semi-transparent mirror in the semi-transparent mirror box unit 91, a sampling component, an imaging mirror group 13 and an image sensor 14.

[0060] The polarizing microscopy module in this embodiment includes two forms: transmission imaging and reflection imaging.

[0061] In transmission imaging, based on the bright-field microscopy (transmission) module, the transmission sampling optical path assembly also includes a first polarization component and an analyzer component. In reflection imaging, based on the bright-field microscopy (reflection) module, the reflection sampling optical path assembly also includes a second polarization component.

[0062] Specifically, in this embodiment, the first polarization assembly, the second polarization assembly, and the polarization detection assembly all include a polarizer assembly and a quarter-wave plate assembly. The polarizer assembly includes a polarizer and a sliding device, and the quarter-wave plate assembly includes a quarter-wave plate and a sliding device.

[0063] The first polarizer assembly is coaxially disposed between the first light source 1 and the condenser lens 4. The polarizer assembly 2 and the quarter-wave plate assembly 3 of the first polarizer assembly are arranged sequentially, with the polarizer assembly 2 located below the quarter-wave plate assembly 3. The second polarizer assembly is coaxially disposed between the converging lens group 16 and the quarter-panel box. The polarizer assembly 17 and the quarter-wave plate assembly 18 of the second polarizer assembly are arranged sequentially, with the polarizer assembly 17 positioned closer to the converging lens group 16 than the quarter-wave plate assembly 18.

[0064] The polarizer is coaxially disposed between the bisection box 12 and the quarter section box 9. The quarter wave plate assembly 10 and the polarizer assembly 11 of the polarizer are arranged sequentially, with the quarter wave plate assembly 10 located below the polarizer assembly 11.

[0065] Specifically, in transmission imaging, based on the bright-field microscopy (transmission) module, two sets of polarizers, two sets of quarter-wave plates and their respective sliding devices are added and located on the front and rear sides of the sampling component. The first polarizer is used for polarization, and the polarizer is used for polarization detection, which together adjust the polarization imaging parameters.

[0066] In reflection imaging, based on the bright-field microscopy (reflection) module, two sets of polarizers, two sets of quarter-wave plates and their respective sliding devices are added. The second polarizing component is located behind the second light source 15, and the polarizing analyzer is located behind the sampling component. They are used for polarizing and polarizing respectively, and together they adjust the polarization imaging parameters.

[0067] It is understood that the polarizer, quarter-wave plate, and their respective sliding devices in this embodiment are only used in the optical path of the polarizing microscope imaging module. The polarizer and quarter-wave plate are slid into the optical path by turning the knob. In other spectral modes, the polarizer and quarter-wave plate need to be slid out of the optical path by turning the knob.

[0068] The fluorescence microscopy module in this embodiment uses reflection imaging.

[0069] like Figure 6 and Figure 7 As shown, based on the bright-field microscopy (reflection) module, a second light source 15 is used as the fluorescence excitation source, and a filter 95 and a fluorescence dichroic mirror box unit 92 are added. The filter 95 is used to selectively transmit excitation light of a specific wavelength, and the fluorescence dichroic mirror of the fluorescence dichroic mirror box unit 92 is used to deflect the fluorescence excitation light path. After the short-wavelength excitation light is reflected, it is focused on the tissue section by the objective lens assembly 8, which excites long-wavelength fluorescence. The fluorescence is collected by the objective lens assembly 8, transmitted through the fluorescence dichroic mirror, and reflected by the mirror in the reflection mirror box unit 121 and imaged onto the image sensor 14 by the imaging mirror group 13.

[0070] As an optional implementation method, such as Figure 1 As shown, the micro Raman spectroscopy detection module in this embodiment also includes an optical fiber translation adjustment frame 20, an optical fiber coupler 21, and a positioning LED light source 23, which are sequentially arranged above the split mirror box 12. The optical fiber coupler 21 includes a main body and an optical fiber bundle 22 connected to the main body. The main body is coaxially arranged with the optical fiber translation adjustment frame 20 and the positioning LED light source 23. The optical fiber bundle 22 is arranged between the main body and the positioning LED light source 23.

[0071] like Figure 8 As shown, the fiber bundle 22 in this embodiment adopts a Y-shaped fiber bundle, including Raman collecting fiber 221 and positioning light fiber 222 arranged in a "Y" shape with Raman collecting fiber 221.

[0072] Among them, the Raman collecting fiber 221 is connected to the Raman spectrometer 24; the positioning light fiber 222 is connected to the positioning LED light source 23, and the positioning light fiber 222 is arranged in a ring around the Raman collecting fiber 221, and the cross-sectional area of ​​the positioning light fiber 222 is smaller than the cross-sectional area of ​​the Raman collecting fiber 221.

[0073] The micro Raman spectroscopy detection module in this embodiment adopts backscattering.

[0074] like Figure 9 As shown, the optical path includes laser 19, Raman dichroism mirror in Raman dichroism mirror box unit 93, sampling assembly, fiber coupler 21, Y-shaped fiber bundle 22, and Raman spectrometer 24. Short-wavelength excitation light exits laser 19 and enters this multimodal microscopic spectral imaging system. The Raman dichroism mirror in Raman dichroism mirror box unit 93 reflects the short-wavelength excitation light, which is then focused onto the tissue slice by objective lens assembly 8, exciting long-wavelength Raman scattered light. This long-wavelength scattered light is collected by the objective lens in objective lens assembly 8, then transmitted through the Raman dichroism mirror in Raman dichroism mirror box unit 93, coupled into the fiber optic cable 21, and connected to the Raman spectrometer 24 for spectral measurement. During operation, positioning LED light source 23 generates a ring-shaped positioning dot array to adjust the fiber optic translation adjustment frame 20, ensuring efficient coupling of Raman scattered light into the fiber optic cable.

[0075] This multimodal microscopic spectral imaging integrated detection system, through the setting of four modules—bright-field microscopy (transmission / reflection), polarized light microscopy (transmission / reflection), fluorescence microscopy (reflection), and micro-Raman spectral acquisition (backscattering)—shares a common tissue section sampling optical path component. By performing spectral imaging on the same area of ​​biological tissue sections, it can achieve multimodal information complementarity, enrich the differential information between cancerous tissues and normal, inflammatory lesions, etc., and can significantly improve the speed of pathological examination and diagnostic efficiency, enabling rapid and accurate intraoperative cancer diagnosis.

[0076] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A multimodal microscopic spectral imaging integrated detection system, characterized in that: The device includes at least two of the following: a bright-field microscopy imaging module that shares a common optical path component for tissue section sampling; a polarized light microscopy imaging module; a fluorescence microscopy imaging module; and a micro-Raman spectroscopy detection module. The bright-field microscopy imaging module, the polarized light microscopy imaging module, and the fluorescence microscopy imaging module share an imaging optical path component. The micro-Raman spectroscopy detection module includes a Raman spectrometer and a laser for irradiating the tissue section with excitation Raman scattering light.

2. The multimodal microscopic spectral imaging integrated detection system according to claim 1, characterized in that, The sampling optical path component includes: The components arranged from bottom to top along the first axis are: a first light source, a condenser lens, a sampling assembly, a quarter lens box, and a half lens box; and A second light source, a converging lens group, and a laser are arranged along a second axis perpendicular to the first axis, with the laser and the converging lens group respectively disposed on both sides of the quarter-lens box.

3. The multimodal microscopic spectral imaging integrated detection system according to claim 2, characterized in that, The sampling optical path assembly also includes: The first polarization component is coaxially disposed between the first light source and the condenser lens; A polarization analyzer is coaxially disposed between the split lens box and the quarter lens box.

4. The multimodal microscopic spectral imaging integrated detection system according to claim 3, characterized in that, The sampling optical path assembly also includes: The second polarization assembly is coaxially disposed between the converging lens group and the quarter lens box.

5. The multimodal microscopic spectral imaging integrated detection system according to any one of claims 2-4, characterized in that: The quarter-lens box includes a semi-transparent and semi-reflective mirror box unit, a fluorescence dichroic mirror box unit, a Raman dichroic mirror box unit, and a first empty box unit, wherein: The semi-transparent and semi-reflective mirrors in the semi-transparent and semi-reflective mirror box unit and the fluorescent dichroic mirrors in the fluorescent dichroic mirror box unit are arranged in the same direction; The Raman dichroic mirror in the Raman dichroic mirror box unit is set to form a 90° angle with the semi-transparent and semi-reflective mirror and the fluorescent dichroic mirror; A filter is provided inside the fluorescent dichroic mirror box unit.

6. The multimodal microscopic spectral imaging integrated detection system according to any one of claims 2-4, characterized in that: The sampling component includes: Objective lens assembly; The stage, located below the objective lens assembly, includes a stage body capable of moving along the XY axis and a lifting mechanism connected to the stage body for enabling the stage to move along the Z axis. A tablet compression device is mounted on the stage.

7. The multimodal microscopic spectral imaging integrated detection system according to any one of claims 2-4, characterized in that, The imaging optical path assembly includes: an imaging lens group and an image sensor arranged coaxially, wherein the imaging lens group is disposed between the image sensor and the bipartite box.

8. The multimodal microscopic spectral imaging integrated detection system according to any one of claims 2-4, characterized in that: The micro Raman spectroscopy detection module further includes an optical fiber translation adjustment frame, an optical fiber coupler, a positioning LED light source, and a Raman spectrometer, which are sequentially arranged above the split-lens box. The optical fiber coupler includes a main body and an optical fiber bundle connected to the main body. The main body, the optical fiber translation adjustment frame, and the positioning LED light source are coaxially arranged. The optical fiber bundle is arranged between the main body and the positioning LED light source.

9. The multimodal microscopic spectral imaging integrated detection system according to claim 8, characterized in that: The fiber bundle is a Y-type fiber bundle, including: A Raman collecting fiber is connected to the Raman spectrometer; The positioning light fiber is arranged in a "Y" shape with the Raman collecting fiber and connected to the positioning LED light source. The positioning light fiber is arranged in a ring around the Raman collecting fiber, and the cross-sectional area of ​​the positioning light fiber is smaller than that of the Raman collecting fiber.

10. The multimodal microscopic spectral imaging integrated detection system according to any one of claims 2-4, characterized in that: The split-lens box includes a reflector box unit and a second empty box unit.

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