Continuous purification chromatography column

Abstract

The utility model relates to chromatography column technical field especially, it is a kind of continuous purification chromatography column, the continuous purification chromatography column includes: base, base top two ends fixed connection first support, middle part fixed connection second support, two first support and second support are evenly installed chromatography column body between;Symmetrical installation multiple sets of clamps between first support and second support, first support inside installation rotating assembly, for driving clamping and releasing chromatography column body of clamp;The symmetrical fixed level bubble of base upper surface, four corners install leveling assembly, for adjusting base level, base bottom installs multiple sets of self-locking universal wheel;The continuous purification chromatography column provided by the utility model has the advantages of installation fixed convenient efficient, base leveling accurate and fast, equipment moves and is fixed flexible and stable, can improve operation convenience, work efficiency and purification quality, reduce maintenance cost, and it is strong practicality.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, and in particular to a continuous purification chromatography column. Background Technology

[0002] Continuous purification chromatography columns are core separation equipment widely used in biopharmaceutical, chemical analysis, and food engineering fields. They achieve efficient separation and purification of different substances by utilizing the differences in the partition coefficients of components in a mixture between the stationary and mobile phases. In biopharmaceuticals, continuous purification chromatography columns can be used to separate and purify active ingredients such as proteins and peptides from complex bio-fermentation broths. In chemical analysis, they enable precise separation of components in mixtures for qualitative and quantitative analysis. This equipment significantly improves separation efficiency and reduces production costs, making it of great significance to the development of related industries.

[0003] However, existing continuous purification chromatography columns still have many shortcomings in practical applications. First, traditional fixing methods mostly use bolt fastening or complex snap-fit ​​structures. When maintaining, replacing, or cleaning the chromatography column, operators need to spend a lot of time and effort disassembling numerous parts, making the operation process cumbersome. Moreover, repeated disassembly and assembly can easily lead to problems such as stripped bolts and worn snap-fits, which not only increases maintenance costs but may also affect the stability of subsequent installations, reducing the service life and operational reliability of the equipment. Second, in terms of base leveling, existing devices lack convenient and precise leveling structures. Relying on manual experience for adjustment makes it difficult to ensure that the base is in a precise level state, causing the chromatography column to tilt. This results in uneven liquid flow within the column, reducing the efficiency and purity of sample purification.

[0004] Therefore, it is necessary to provide a new continuous purification chromatography column to solve the above-mentioned technical problems. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a continuous purification chromatography column.

[0006] The continuous purification chromatography column provided by this utility model includes: a base, with first supports fixedly connected to both ends of the top of the base, and a second support fixedly connected to the middle of the top of the base; a chromatography column body is installed between the two first supports and the second support; multiple sets of clamping plates are symmetrically installed between the first supports and the second support, and the clamping plates are used to clamp the chromatography column body; a rotating assembly is installed inside the first support, and the rotating assembly is used to drive the clamping plates to clamp and release the chromatography column body; leveling bubbles are symmetrically fixedly connected to the upper surface of the base, and leveling assemblies are installed at the four corners of the base, and the leveling assemblies are used to adjust the base to make the base level.

[0007] Preferably, the rotating assembly includes: a slide groove, a threaded slider, a first threaded rod, a guide slider, a guide rod, and a connecting rod; slide grooves are equidistantly provided on opposite sides of the first and second supports; a threaded slider is symmetrically slidably connected in the middle slide groove of the first support; a first threaded rod is rotatably connected in the middle slide groove of the first support; both ends of the first threaded rod are threadedly connected to the threaded slider; guide sliders are symmetrically slidably connected in the slide grooves at the top and bottom of the first support and in the slide grooves of the second support; guide rods are fixedly connected in the slide grooves at the top and bottom of the first support and in the slide grooves of the second support; the guide rods are slidably connected to the guide sliders; a connecting rod is symmetrically slidably connected inside the first support; and the threaded slider is fixed to the guide slider via the connecting rod.

[0008] Preferably, the leveling component includes: a second threaded rod and a support plate; the base is threaded with the second threaded rod at each of the four corners, and the bottom of the second threaded rod is fixedly connected to the support plate.

[0009] Preferably, one end of the first threaded rod extends out of the first bracket and is fixedly connected to a swivel.

[0010] Preferably, the first threaded rod is a bidirectional threaded rod.

[0011] Preferably, both the threaded slider and the guide slider are fixedly connected to the clamping plate.

[0012] Preferably, a throttle is fixedly connected to the top of the second threaded rod.

[0013] Preferably, multiple sets of self-locking casters are symmetrically installed on the bottom of the base.

[0014] Compared with related technologies, the continuous purification chromatography column provided by this utility model has the following beneficial effects:

[0015] Convenient and efficient chromatography column installation and fixation:

[0016] Regarding the installation and fixation of the chromatography column body, this invention features a unique rotating assembly. When installing the chromatography column body, the operator only needs to rotate the ring at one end of the first threaded rod. The bidirectional threaded rod allows the threaded slider to move in opposite directions along the guide rod within the groove. The connecting rod drives the guide slider and clamping plate to move synchronously, achieving rapid clamping and fixation of the chromatography column body. This structure eliminates the need for cumbersome bolt disassembly and assembly, significantly reducing installation time and the operator's workload. When maintaining, replacing, or cleaning the chromatography column, simply rotate the ring in the opposite direction to easily release the clamping plate, achieving rapid disassembly and greatly improving operational convenience. Furthermore, since repeated bolt disassembly and assembly are unnecessary, problems such as bolt stripping and clip wear are avoided, effectively extending the equipment's service life while ensuring the stability of subsequent installations and improving the reliability of equipment operation.

[0017] Precise and efficient base leveling:

[0018] To address the challenge of leveling the base, this invention features a specialized leveling assembly. The structure formed by the second threaded rods at the four corners of the base and the support plate, along with symmetrically arranged bubble levels on the upper surface, makes leveling simple and precise. Operators can easily adjust the height of the four corners of the base by rotating the handle at the top of the second threaded rod, based on the bubble level indicators, ensuring the base quickly reaches a horizontal position and thus keeping the chromatography column vertical. Compared to traditional methods relying on manual experience, this structure significantly improves leveling efficiency and accuracy, ensuring uniform and stable liquid flow within the column during chromatography, thereby effectively enhancing sample purification efficiency and purity.

[0019] Flexible and stable equipment movement and fixing:

[0020] Regarding the movement and fixation functions of the equipment, this utility model features multiple sets of self-locking casters symmetrically installed at the bottom of the base. During equipment transfer, the locking device of the casters is unlocked, allowing the casters to rotate freely and enabling the equipment to move flexibly. This effectively avoids the problem of jamming during movement of traditional devices and improves the efficiency of equipment handling. Once the equipment reaches the designated location, the casters can be locked by operating the self-locking device, which can securely fix the equipment in the corresponding position. Compared with traditional fixing methods, this method offers greater stability and effectively eliminates the safety hazard of displacement caused by external forces during use, providing a stable and reliable working environment for chromatography purification. Attached Figure Description

[0021] Figure 1 A schematic diagram of the structure of the continuous purification chromatography column provided by this utility model;

[0022] Figure 2 for Figure 1 A schematic diagram of the cross-sectional structure of the first support shown;

[0023] Figure 3 for Figure 2 The diagram shows the structure of part A.

[0024] Figure 4 for Figure 2 The diagram shows the structure of structure B.

[0025] Figure 5 for Figure 2 The diagram shows the structure of the second threaded rod.

[0026] The following are the labels in the diagram: 1. Base; 2. First support; 3. Second support; 4. Chromatography column body; 5. Clamping plate; 6. Leveling bubble; 7. Slide groove; 8. Threaded slider; 9. First threaded rod; 10. Guide slider; 11. Guide rod; 12. Connecting rod; 13. Second threaded rod; 14. Support plate; 15. Rotary ring; 16. Turning handle; 17. Self-locking caster wheel. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely for explaining the present utility model and are not intended to limit the present utility model.

[0028] The specific implementation of this utility model will be described in detail below with reference to specific embodiments.

[0029] Please see Figures 1 to 5 A continuous purification chromatography column includes: a base 1, with first supports 2 fixedly connected to both ends of the top of the base 1, and a second support 3 fixedly connected to the middle of the top of the base 1; a chromatography column body 4 is installed between the two first supports 2 and the second support 3; multiple sets of clamping plates 5 are symmetrically installed between the first supports 2 and the second support 3, and the clamping plates 5 are used to clamp the chromatography column body 4; a rotating assembly is installed inside the first support 2, and the rotating assembly is used to drive the clamping plates 5 to clamp and release the chromatography column body 4; a leveling bubble 6 is symmetrically fixedly connected to the upper surface of the base 1; a leveling assembly is installed at each of the four corners of the base 1, and the leveling assembly is used to adjust the base 1 to make the base 1 horizontal; multiple sets of self-locking casters 17 are symmetrically installed at the bottom of the base 1; the leveling assembly includes: a second threaded rod 13 and a support plate 14; a second threaded rod 13 is threadedly connected to each of the four corners of the base 1, and a support plate 14 is fixedly connected to the bottom of each second threaded rod 13; a handle 16 is fixedly connected to the top of each second threaded rod 13.

[0030] It should be noted that the operator can move this device using the self-locking casters 17; the chromatography column body 4 is perpendicular to the base 1.

[0031] Please see Figures 2 to 4The rotating assembly includes: a slide groove 7, a threaded slider 8, a first threaded rod 9, a guide slider 10, a guide rod 11, and a connecting rod 12; slide grooves 7 are equidistantly provided on opposite sides of the first bracket 2 and the second bracket 3; a threaded slider 8 is symmetrically slidably connected in the middle slide groove 7 of the first bracket 2; a first threaded rod 9 is rotatably connected in the middle slide groove 7 of the first bracket 2; both ends of the first threaded rod 9 are threadedly connected to the threaded slider 8; guide sliders 10 are symmetrically slidably connected in the top and bottom slide grooves 7 of the first bracket 2 and the slide groove 7 of the second bracket 3; guide rods 11 are fixedly connected in the top and bottom slide grooves 7 of the first bracket 2 and the slide groove 7 of the second bracket 3; guide rods 11 are slidably connected to guide sliders 10; connecting rods 12 are symmetrically slidably connected inside the first bracket 2; the threaded slider 8 is fixed to the guide slider 10 through the connecting rod 12; one end of the first threaded rod 9 extends out of the first bracket 2 and is fixedly connected to a rotating ring 15; the first threaded rod 9 is a bidirectional threaded rod; both the threaded slider 8 and the guide slider 10 are fixedly connected to the clamping plate 5.

[0032] It should be noted that the connecting rod 12 can make the guide slider 10 and the threaded slider 8 move synchronously, and the guide rod 11 guides the movement of the guide slider 10.

[0033] The working principle of the continuous purification chromatography column provided by this utility model is as follows:

[0034] Chromatography column body 4 Installation and fixing:

[0035] After placing the chromatography column body 4 in the corresponding position between the two first supports 2 and the second support 3, the fixing process is started. The operator rotates the swivel ring 15 at one end of the first threaded rod 9. Since the first threaded rod 9 is a bidirectional threaded rod, its two ends are respectively threadedly connected to the threaded sliders 8 symmetrically arranged in the slide groove 7 in the middle of the first support 2. As the first threaded rod 9 rotates, according to the principle of thread transmission, the two threaded sliders 8 will move towards or away from each other in the slide groove 7 along the direction of the guide rod 11. At the same time, guide sliders 10 are symmetrically slidably connected in the slide grooves 7 at the top and bottom of the first support 2 and the slide groove 7 of the second support 3. These guide sliders 10 are fixedly connected to the corresponding slide grooves 7. The guide rod 11 slides to ensure that the slider can only move along the direction of the guide rod 11. The key is that both the threaded slider 8 and the guide slider 10 are fixedly connected to the clamping plate 5, and the connecting rod 12, which is symmetrically slidably connected inside the first bracket 2, connects the threaded slider 8 and the guide slider 10. When the threaded slider 8 moves, it will drive the guide slider 10 to move synchronously through the connecting rod 12, thereby driving the clamping plate 5 to clamp or release the chromatography column body 4. By controlling the rotation angle of the rotating ring 15, the operator can accurately adjust the clamping force of the clamping plate 5 on the chromatography column body 4, ensuring that the chromatography column is stable and does not shake during subsequent work, providing a stable basic environment for the efficient separation and purification of samples.

[0036] Base 1 horizontal adjustment:

[0037] When it is necessary to level the base 1 to ensure that the chromatography column 4 is vertical, the operator starts adjusting by rotating the handle 16 at the top of the second threaded rod 13. Because the base 1 is threaded with the second threaded rod 13 at each of its four corners, rotating the second threaded rod 13 will cause it to move up and down on the base 1 according to the characteristics of thread transmission. The support plate 14, which is fixedly connected to the bottom of the second threaded rod 13, will also rise and fall synchronously. The level bubble 6, which is symmetrically fixed on the upper surface of the base 1, is the core basis for judging the level state. Once the base 1 is tilted, the bubble in the level bubble 6 will deviate from the center position. The operator continuously observes the level bubble 6 and continuously adjusts the lifting height of the second threaded rod 13 at the four corners until the level bubble 6 is in the center position. At this time, the base 1 is in a horizontal state, so that the chromatography column 4 installed on the support of the base 1 remains vertical. This ensures that the liquid flows evenly and stably in the column during the chromatography process, avoids abnormal liquid flow caused by the tilt of the base 1, and thus improves the efficiency and quality of sample purification.

[0038] Equipment relocation and installation:

[0039] Multiple sets of self-locking casters 17 symmetrically installed at the bottom of the base 1 provide the continuous purification chromatography column with excellent mobility. When the equipment needs to be moved to a designated location in the work area, the operator unlocks the locking device of the self-locking casters 17, at which point the casters can rotate freely. By pushing the equipment, the rolling of the casters enables flexible displacement of the equipment. When the equipment reaches the predetermined position, the operator operates the self-locking device again to lock the casters, preventing them from rotating freely, thus firmly fixing the equipment in the corresponding position and preventing displacement of the equipment due to external interference during subsequent use, creating a stable working environment for chromatography purification.

[0040] The working principle of the chromatography column itself:

[0041] A chromatography column is packed with a stationary phase, commonly including adsorbents, ion exchange resins, and gels. Different stationary phases are suitable for separating different types of mixtures. The mixture to be separated is carried by a mobile phase (such as a specific solvent or buffer solution) and continuously injected from the top of the column. Due to differences in the interaction forces between the components in the mixture and the stationary phase (such as adsorption force, ion exchange capacity, and molecular size exclusion effect), the partition coefficients of each component differ between the stationary and mobile phases. Components with larger partition coefficients have stronger adsorption or retention capacity on the stationary phase and migrate more slowly within the column; components with smaller partition coefficients migrate more easily with the mobile phase. Within the chromatography column, the components move rapidly. As the mobile phase flows downwards, the components repeatedly undergo partitioning, adsorption, and desorption processes between the stationary and mobile phases. These differences are gradually amplified, and the originally mixed components gradually separate into different bands within the chromatography column. Finally, the components elute from the bottom of the column in sequence. By collecting the eluent at different time points, the mixture can be separated and purified. The continuous purification chromatography column, with its stable fixed structure and precisely leveled base 1, ensures the stability and uniformity of liquid flow during chromatography, avoiding the deterioration of separation effect due to external factors, thereby improving separation efficiency and purity.

[0042] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A continuous purification chromatography column, characterized in that, include: A base (1) is fixedly connected to two ends of the top of the base (1) with a first bracket (2) and a second bracket (3) is fixedly connected to the middle of the top of the base (1). A chromatography column body (4) is installed between the two first brackets (2) and the second bracket (3). Multiple sets of clamping plates (5) are symmetrically installed between the first brackets (2) and the second brackets (3). The clamping plates (5) are used to clamp the chromatography column body (4). A rotating component is installed inside the first bracket (2). The rotating component is used to drive the clamping plates (5) to clamp and release the chromatography column body (4). A leveling bubble (6) is symmetrically fixedly connected to the upper surface of the base (1). A leveling component is installed at each of the four corners of the base (1). The leveling component is used to adjust the base (1) so that the base (1) is level.

2. The continuous purification chromatography column according to claim 1, characterized in that, The rotating assembly includes: a slide groove (7), a threaded slider (8), a first threaded rod (9), a guide slider (10), a guide rod (11), and a connecting rod (12); the first bracket (2) and the second bracket (3) are provided with slide grooves (7) at equal intervals on opposite sides; the threaded slider (8) is symmetrically slidably connected in the middle slide groove (7) of the first bracket (2); the first threaded rod (9) is rotatably connected in the middle slide groove (7) of the first bracket (2); and the two ends of the first threaded rod (9) are threadedly connected to the threaded slider (8). Guide sliders (10) are symmetrically slidably connected in the top and bottom grooves (7) of the first bracket (2) and the grooves (7) of the second bracket (3). Guide rods (11) are fixedly connected in the top and bottom grooves (7) of the first bracket (2) and the grooves (7) of the second bracket (3). The guide rods (11) are slidably connected to the guide sliders (10). Connecting rods (12) are symmetrically slidably connected inside the first bracket (2). The threaded slider (8) is fixed to the guide slider (10) through the connecting rods (12).

3. The continuous purification chromatography column according to claim 1, characterized in that, The leveling assembly includes: a second threaded rod (13) and a support plate (14); the base (1) is threaded with the second threaded rod (13) at each of the four corners, and the bottom of the second threaded rod (13) is fixedly connected with the support plate (14).

4. The continuous purification chromatography column according to claim 2, characterized in that, One end of the first threaded rod (9) extends out of the first bracket (2) and is fixedly connected to a swivel (15).

5. The continuous purification chromatography column according to claim 2, characterized in that, The first threaded rod (9) is a bidirectional threaded rod.

6. The continuous purification chromatography column according to claim 2, characterized in that, Both the threaded slider (8) and the guide slider (10) are fixedly connected to the clamp (5).

7. The continuous purification chromatography column according to claim 3, characterized in that, The top of each of the second threaded rods (13) is fixedly connected to a throttle (16).

8. The continuous purification chromatography column according to claim 1, characterized in that, Multiple sets of self-locking casters (17) are symmetrically installed on the bottom of the base (1).

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