An anaerobic microbial culture medium mixing device

Abstract

This utility model discloses an anaerobic microbial culture medium mixing device, relating to the field of culture medium mixing. It includes a mixing tank with a detachable top cover. A closed cap protruding upwards is formed at the center of the top plate of the mixing tank. The interior of the mixing tank has a hollow structure. An electrical socket is installed on the outer wall of the closed cap. A vacuum mechanism communicating with the inner cavity of the mixing tank is installed on the top of the top cover. A pressure-replenishing mechanism communicating with the inner cavity of the top cover is also installed on the top of the mixing tank. The vacuum mechanism and the pressure-replenishing mechanism are symmetrically distributed above the top plate of the top cover. A discharge pipe with a discharge valve is installed at the bottom of the mixing tank. This utility model, by setting up the vacuum mechanism and the pressure-replenishing mechanism, can extract the gas inside the device and replenish it with nitrogen at a rated pressure, thereby preventing oxygen from being mixed into the culture medium during mixing.

Description

Technical Field

[0001] This utility model relates to the field of culture medium mixing, specifically an anaerobic microbial culture medium mixing device. Background Technology

[0002] When preparing culture media for anaerobic microorganisms, care must be taken to avoid large amounts of oxygen from being mixed into the media. In existing technologies, slow stirring or making the container of the device narrower is generally used to reduce the contact surface with oxygen, thereby avoiding the above-mentioned problems.

[0003] However, the above method still inevitably results in some oxygen being mixed into the culture medium raw materials being stirred during the stirring process. Utility Model Content

[0004] The purpose of this invention is to provide an anaerobic microbial culture medium mixing device in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an anaerobic microbial culture medium mixing device, comprising a mixing tank, a top cover detachably connected to the top of the mixing tank, a closed cover protruding upward formed at the center of the top plate of the mixing tank, the interior of the mixing tank having a hollow structure, an electrical socket installed on the outer wall of the closed cover, an air extraction mechanism communicating with the inner cavity of the mixing tank installed on the top of the top cover, a pressure replenishing mechanism communicating with the inner cavity of the top cover installed on the top of the mixing tank, the air extraction mechanism and the pressure replenishing mechanism being symmetrically distributed above the top plate of the top cover, a discharge pipe installed at the bottom of the mixing tank, and a discharge valve installed on the discharge pipe.

[0006] As a further embodiment of this utility model: the air extraction mechanism includes a vertical pipe extending vertically from above the top plate of the upper cover to below the bottom plate of the upper cover. A horizontally protruding guide pipe is integrally formed in the middle of the vertical pipe. A solenoid valve is installed in the inner cavity of the upper cover on the vertical pipe. A one-way air inlet valve is installed above the solenoid valve on the vertical pipe. The one-way air inlet valve is located below the guide pipe. A one-way exhaust valve is installed above the one-way air inlet valve on the vertical pipe. The one-way exhaust valve is located above the guide pipe.

[0007] As a further embodiment of this utility model: the air extraction mechanism further includes a piston slidably installed in the inner cavity of the guide tube, one end of the piston is equipped with a piston rod extending to the outside of the guide tube, the end of the piston rod located outside the guide tube is rotatably mounted with a connecting rod, the connecting rod is rotatably connected to one end of the rotating disk at an eccentric position, and one end of the rotating disk is coaxially and fixedly connected to the output shaft of the rotary motor.

[0008] As a further embodiment of this utility model: the pressure replenishment mechanism includes a gas supply pipe extending from the top of the upper cover plate to the bottom of the upper cover plate. A first pressure sensor is installed at the input end of the gas supply pipe, and an air inlet pipe is installed at the output end of the first pressure sensor. A second one-way air inlet valve is installed on the air inlet pipe. A second pressure sensor is installed outside the first pressure sensor, and the sensing end of the second pressure sensor is located inside the nitrogen tank.

[0009] As a further embodiment of this utility model: a stirring motor is installed at the top of the bottom plate of the upper cover, and a stirrer is installed at the output end of the stirring motor, which penetrates into the inner cavity of the stirring tank. The stirrer is rotatably connected to the upper cover through a bearing, and the stirring motor is electrically connected to the electrical socket through a wire.

[0010] As a further embodiment of this utility model: a first pressure sensor is installed above the top plate of the upper cover, penetrating into the inner cavity of the mixing tank, and the first pressure sensor is electrically connected to the first solenoid valve and the second solenoid valve through a controller.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. By setting up a gas extraction mechanism and a pressure replenishment mechanism, the gas inside the device can be extracted and nitrogen gas at the rated pressure can be added, thereby avoiding the introduction of oxygen into the culture medium during the stirring process. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model;

[0014] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0015] Figure 3 This is a schematic diagram of the piston installation of this utility model;

[0016] Figure 4 This is a schematic diagram of the installation of the No. 2 solenoid valve of this utility model.

[0017] In the diagram: 1. Mixing tank; 2. Top cover; 3. Pressure sensor No. 1; 4. Gas supply pipe; 5. Nitrogen tank; 6. Inlet pipe; 7. Pressure sensor No. 2; 8. Sealing cover; 9. Vertical pipe; 10. Electrical socket; 11. Mixing motor; 12. Agitator; 13. Discharge pipe; 14. Discharge valve; 15. Guide pipe; 16. Piston; 17. Piston rod; 18. Connecting rod; 19. Rotary motor; 20. One-way exhaust valve; 21. One-way inlet valve No. 1; 22. Solenoid valve No. 1; 23. Solenoid valve No. 2; 24. Rotary disc. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1-4 In this embodiment of the present invention, an anaerobic microbial culture medium mixing device includes a mixing tank 1. The top of the mixing tank 1 is detachably connected to a top cover 2. A closed cover 8 protruding upward is formed at the center of the top plate of the mixing tank 1. The interior of the mixing tank 1 is hollow. An electrical socket 10 is installed on the outer wall of the closed cover 8. An air extraction mechanism communicating with the inner cavity of the mixing tank 1 is installed on the top of the top cover 2. A pressure replenishing mechanism communicating with the inner cavity of the top cover 2 is installed on the top of the mixing tank 1. The air extraction mechanism and the pressure replenishing mechanism are symmetrically distributed above the top plate of the top cover 2. A discharge pipe 13 is installed at the bottom of the mixing tank 1. A discharge valve 14 is installed on the discharge pipe 13.

[0020] In this embodiment: First, before the upper cover 2 is connected to the mixing tank 1, the culture medium raw material is added to the mixing tank 1. After the addition is completed, the upper cover 2 is connected to the top of the mixing tank 1 by rotating the bolts and nuts. Then, the suction mechanism is started to extract the gas inside the device. When the gas inside the mixing tank 1 reaches the preset minimum gas pressure, the suction mechanism stops running. Then, the pressurization mechanism pressurizes the device with nitrogen to compensate the gas pressure inside the tank to normal pressure. In this way, oxygen can be avoided from mixing into the culture medium liquid raw material during the stirring process. After the stirring is completed, the stirred raw material can be discharged by connecting the receiving port of the container to the discharge pipe 13 and opening the discharge valve 14.

[0021] Please refer to this carefully. Figure 1 , Figure 2 and Figure 3The air extraction mechanism includes a vertical pipe 9 extending vertically from the top plate of the upper cover 2 to the bottom plate of the upper cover 2. A horizontally protruding guide pipe 15 is integrally formed in the middle of the vertical pipe 9. A solenoid valve 22 is installed in the inner cavity of the upper cover 2. A one-way air intake valve 21 is installed above the solenoid valve 22 in the vertical pipe 9. The one-way air intake valve 21 is located below the guide pipe 15. A one-way exhaust valve 20 is installed above the one-way air intake valve 21 in the vertical pipe 9. The one-way exhaust valve 20 is located above the guide pipe 15. The air extraction mechanism also includes a piston 16 slidably installed in the inner cavity of the guide pipe 15. A piston rod 17 is installed at one end of the piston 16, extending to the outside of the guide pipe 15. A connecting rod 18 is rotatably installed at the end of the piston rod 17 located outside the guide pipe 15. The connecting rod 18 is rotatably connected to one end of the rotating disk 24 at an eccentric position. One end of the rotating disk 24 is coaxially fixedly connected to the output shaft of the rotary motor 19.

[0022] In this embodiment: when the raw materials are added and the air is being evacuated, the rotary motor 19 is started, which drives the rotating disk 24 to rotate. The rotation of the rotating disk 24 causes one end of the connecting rod 18 to rotate circumferentially. At this time, the other end of the connecting rod 18 pushes the piston rod 17 to slide axially. The piston rod 17 can then drive the piston 16 to move synchronously. When the piston 16 moves toward the rotating disk 24, a suction force is formed. At this time, the air inside the device enters the inner cavity of the guide tube 15 through the first one-way air inlet valve 21. Then, when the piston 16 moves in the opposite direction, the air that has entered the guide tube 15 is discharged outward through the one-way exhaust valve 20, forming the air evacuation function, until the air pressure inside the device reaches the preset minimum air pressure. At this time, the controller can control the first solenoid valve 22 to close.

[0023] Please refer to this carefully. Figure 1 , Figure 2 and Figure 4 The pressurization mechanism includes a gas supply pipe 4 that extends from the top of the top plate of the upper cover 2 to the bottom of the bottom plate of the upper cover 2. A first pressure sensor 3 is installed at the input end of the gas supply pipe 4. An air inlet pipe 6 is installed at the output end of the first pressure sensor 3. A second one-way air inlet valve is installed on the air inlet pipe 6. A second pressure sensor 7 is installed outside the first pressure sensor 3. The sensing end of the second pressure sensor 7 is located in the inner cavity of the nitrogen tank 5. A first pressure sensor 3 that extends from the top plate of the upper cover 2 to the inner cavity of the mixing tank 1 is installed above the top plate of the upper cover 2. The first pressure sensor 3 is electrically connected to the first solenoid valve 22 and the second solenoid valve 23 through a controller.

[0024] In this embodiment: when the internal pressure of the device reaches the preset minimum pressure, the first pressure sensor 3 sends an electrical signal to the controller, which controls the first solenoid valve 22 to close and simultaneously controls the second solenoid valve 23 to open. At this time, the nitrogen in the nitrogen tank 5 is input into the device through the gas supply pipe 4 to fill the device with nitrogen until the internal pressure of the device reaches the rated preset maximum pressure. At this time, the first pressure sensor 3 sends an electrical signal to the controller, which controls the second solenoid valve 23 to close. In this way, the negative pressure splashing phenomenon during stirring and mixing is avoided.

[0025] After the nitrogen in nitrogen tank 5 is output, when it reaches the preset minimum pressure, the second pressure sensor 7 sends an electrical signal to the controller. The controller then controls the delivery air pump to replenish nitrogen in nitrogen tank 5 until the nitrogen pressure reaches the rated preset maximum pressure. At this point, the second pressure sensor 7 sends an electrical signal to the controller, and the controller then controls the air pump to stop running.

[0026] Please refer to this carefully. Figure 1 A stirring motor 11 is installed at the top of the bottom plate of the upper cover 2. A stirrer 12 that penetrates into the inner cavity of the mixing tank 1 is installed at the output end of the stirring motor 11. The stirrer 12 is rotatably connected to the upper cover 2 through a bearing. The stirring motor 11 is electrically connected to the electrical socket 10 through a wire.

[0027] In this embodiment: During stirring, the stirring motor 11 is started, and the stirring motor 11 drives the stirrer 12 to stir the culture medium raw materials. In order to avoid air leakage at the shaft and rotating connection of the stirrer 12, the stirring motor 11 is built into the inside of the upper cover 2 and powered through the electrical socket 10. The heat generated by the motor operation is dissipated outward through the metal upper cover 2.

[0028] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An anaerobic microbial culture medium mixing device, comprising a mixing tank (1), characterized in that, The top of the mixing tank (1) is detachably connected to a top cover (2). A closed cover (8) protruding upward is formed at the center of the top plate of the mixing tank (1). The interior of the mixing tank (1) is hollow. An electrical socket (10) is installed on the outer wall of the closed cover (8). An air extraction mechanism connected to the inner cavity of the mixing tank (1) is installed on the top of the top cover (2). A pressure replenishing mechanism connected to the inner cavity of the top cover (2) is installed on the top of the mixing tank (1). The air extraction mechanism and the pressure replenishing mechanism are symmetrically distributed above the top plate of the top cover (2). A discharge pipe (13) is installed at the bottom of the mixing tank (1). A discharge valve (14) is installed on the discharge pipe (13).

2. The anaerobic microbial culture medium mixing and stirring device according to claim 1, characterized in that, The air extraction mechanism includes a vertical pipe (9) that extends vertically from the top plate of the upper cover (2) to the bottom plate of the upper cover (2). A horizontally protruding guide pipe (15) is integrally formed in the middle of the vertical pipe (9). A solenoid valve (22) is installed in the inner cavity of the upper cover (2) on the vertical pipe (9). A one-way air inlet valve (21) is installed above the solenoid valve (22) on the vertical pipe (9). The one-way air inlet valve (21) is located below the guide pipe (15). A one-way exhaust valve (20) is installed above the one-way air inlet valve (21) on the vertical pipe (9). The one-way exhaust valve (20) is located above the guide pipe (15).

3. The anaerobic microbial culture medium mixing and stirring device according to claim 2, characterized in that, The air extraction mechanism also includes a piston (16) slidably installed in the inner cavity of the guide tube (15). One end of the piston (16) is equipped with a piston rod (17) that extends through to the outside of the guide tube (15). One end of the piston rod (17) located outside the guide tube (15) is rotatably equipped with a connecting rod (18). The connecting rod (18) is rotatably connected to one end of the rotating disk (24) at an eccentric position. One end of the rotating disk (24) is coaxially fixedly connected to the output shaft of the rotary motor (19).

4. The anaerobic microbial culture medium mixing and stirring device according to claim 3, characterized in that, The pressure replenishment mechanism includes a gas supply pipe (4) extending from the top plate of the upper cover (2) to the bottom plate of the upper cover (2). A first pressure sensor (3) is installed at the input end of the gas supply pipe (4). An air inlet pipe (6) is installed at the output end of the first pressure sensor (3). A second one-way air inlet valve is installed on the air inlet pipe (6). A second pressure sensor (7) is installed outside the first pressure sensor (3). The sensing end of the second pressure sensor (7) is located in the inner cavity of the nitrogen tank (5).

5. The anaerobic microbial culture medium mixing and stirring device according to claim 4, characterized in that, A stirring motor (11) is installed at the top of the bottom plate of the upper cover (2). A stirrer (12) is installed at the output end of the stirring motor (11) and extends into the inner cavity of the stirring tank (1). The stirrer (12) is rotatably connected to the upper cover (2) through a bearing. The stirring motor (11) is electrically connected to the electrical socket (10) through a wire.

6. The anaerobic microbial culture medium mixing device according to claim 5, characterized in that, A first pressure sensor (3) is installed above the top plate of the cover (2) and extends into the inner cavity of the mixing tank (1). The first pressure sensor (3) is electrically connected to the first solenoid valve (22) and the second solenoid valve (23) through a controller.

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