Adjustable cell filter

Abstract

This utility model discloses an adjustable cell filter, including a filter cover and a connecting sleeve disposed at the bottom of the filter cover. The top of the filter cover is open, and a retaining ring is fixed to the lower circular inner wall of the center of the filter cover. A placement ring is placed at the top of the retaining ring, and a cylindrical filter frame is fixed inside the placement ring. The top of the cylindrical filter frame is open. By adding a novel flow guiding and anti-splash device inside the upper side of the center of the filter cover, located above the cell filter membrane, when tissue fluid is poured in, it is poured into the conical flow guiding and anti-splash cover. Its smooth inner wall allows the tissue fluid to flow slowly, avoiding impact on the cell filter membrane and extending its life. The conical structure can also converge and guide the flow. The flat cylindrical protective cover further buffers the flow, allowing the tissue fluid to fall smoothly onto the cell filter membrane, achieving uniform filtration. The hemispherical diffuser block arches upward to diffuse the tissue fluid, avoiding excessive local pressure that could damage the filter membrane, optimizing distribution, and improving efficiency and quality.

Description

Technical Field

[0001] This invention belongs to the technical field of biological experimental instruments, specifically relating to an adjustable cell filter. Background Technology

[0002] A cell filter is a device used to separate and filter solid particles and impurities from cell samples. It is widely used in clinical testing, biomedical research, and biopharmaceuticals. Cell filters utilize the size of the mesh openings to screen cells or molecules of different sizes. When a mixture of cell samples is passed through the filter, cells or molecules smaller than the mesh openings can pass through, while cells or molecules larger than the mesh openings are blocked above the openings, thus achieving the separation of the target cells or molecules from the mixture. However, traditional cell filters have revealed some problems in practical use.

[0003] When pouring the tissue fluid to be filtered into the filter, due to the lack of effective flow guidance, the tissue fluid often directly impacts the cell filtration membrane at the bottom of the filter. This direct impact not only easily leads to localized damage to the cell filtration membrane, shortening its lifespan and increasing experimental costs, but also causes uneven distribution of the tissue fluid on the cell filtration membrane, affecting the filtration effect, reducing the accuracy and stability of cell filtration, and potentially biasing subsequent cell analysis and research results. Furthermore, there is the problem of liquid splashing during the pouring process. This not only wastes samples and affects the accuracy of experimental data, but the splashed tissue fluid containing cells may also contaminate the experimental environment and pose a potential threat to the safety of experimental personnel. Utility Model Content

[0004] The purpose of this invention is to provide an adjustable cell filter to solve the problems mentioned in the background art, such as the lack of effective flow guidance measures when the cell tissue fluid to be filtered is poured into the filter, resulting in direct impact on the cell filter membrane, causing local damage, shortening its lifespan, and uneven distribution of tissue fluid affecting the filtration effect and accuracy. At the same time, the pouring process causes liquid splashing, resulting in sample waste, affecting data accuracy, and may also pollute the environment and threaten the safety of laboratory personnel.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an adjustable cell filter, comprising a filter cover and a connecting sleeve disposed at the bottom of the filter cover. The top of the filter cover is open, and a retaining ring is fixed to the lower circular inner wall of the center of the filter cover. A placement ring is placed at the top of the retaining ring, and a cylindrical filter frame is fixed inside the placement ring. The top of the cylindrical filter frame is open, and the bottom of the cylindrical filter frame has equally spaced leakage holes. A cell filter membrane is laid flat inside the bottom of the cylindrical filter frame, and the cell filter membrane completely covers the top of the multiple leakage holes. A flow guiding and splash-proof device is disposed inside the upper side of the center of the filter cover.

[0006] Preferably, the flow guiding and splash-proof device includes a handle, a flat cylindrical protective cover, a conical flow guiding and splash-proof cover, and an annular mounting baffle. The annular mounting baffle is placed at the top of the filter cover. A handle is provided on the outer wall of the right end of the annular mounting baffle. The conical flow guiding and splash-proof cover is fixed to the inner wall of the annular mounting baffle. The cone tip of the conical flow guiding and splash-proof cover faces downward, and the opening diameter at the top of the conical flow guiding and splash-proof cover is larger than the opening diameter at the bottom. The conical flow guiding and splash-proof cover is located inside the upper side of the center of the filter cover. The bottom end of the conical flow guiding and splash-proof cover is connected to a flat cylindrical protective cover, and the bottom end of the flat cylindrical protective cover is open.

[0007] Preferably, the flow guiding and splash-proof device further includes thin fixing rods, flat fixing blocks, and hemispherical diffuser blocks. A hemispherical diffuser block is provided directly below the bottom opening of the conical flow guiding and splash-proof cover, and the hemispherical diffuser block is located at the center inside the flat cylindrical protective cover. A flat fixing block is provided at the bottom of the hemispherical diffuser block. Thin fixing rods are fixed at both ends of the flat fixing block, and the two thin fixing rods are respectively fixed to the inner walls of the left and right ends of the flat cylindrical protective cover.

[0008] Preferably, the inner wall of the conical flow guide splash shield is smooth and burr-free, the flat cylindrical protective cover is located above the cylindrical filter frame, and the inner diameter of the flat cylindrical protective cover is equal to the inner diameter of the cylindrical filter frame.

[0009] Preferably, the hemispherical diffuser block is arranged in an upward arched manner below the conical flow guide splash shield, and the outer wall of the hemispherical diffuser block is smooth and burr-free, and the diameter of the hemispherical diffuser block is smaller than the diameter of the bottom opening of the conical flow guide splash shield.

[0010] Preferably, a filter material collection test tube is inserted into the lower half of the connecting sleeve, a base is provided on the outside of the bottom end of the filter material collection test tube, a test tube insertion hole is provided in the center of the base, and the filter material collection test tube is inserted into the test tube insertion hole.

[0011] Preferably, the bottom third of the filter cover is tapered, the filter cover is connected to the inside of the filter material collection tube through a connecting sleeve, and a vacuum tube is provided on the upper side of the center of the right end of the connecting sleeve.

[0012] Preferably, the vacuum tube is connected to an external vacuum pump via a connecting tube, and multiple spare test tube insertion holes are equidistantly arranged on the outer side and inside of the center of the base, and the diameters of the multiple spare test tube insertion holes are different.

[0013] Compared with the prior art, this utility model provides an adjustable cell filter with the following features:

[0014] Beneficial effects:

[0015] This invention features a novel flow-guiding and splash-proof device added to the upper inner side of the center of the filter cover. This device is positioned above the cell filter membrane used for cell filtration. When tissue fluid containing cells is poured onto the cell filter membrane inside the filter cover, the fluid can be directly poured into the conical flow-guiding and splash-proof cover. The smooth inner wall of the conical cover allows the tissue fluid to flow slowly down its inner wall, preventing direct impact on the cell filter membrane and thus extending its lifespan. Furthermore, due to its unique conical structure, the diameter of the top opening is larger than the bottom opening, which helps to initially gather and guide the poured tissue fluid, allowing it to flow more evenly towards the flat cylindrical protective cover below. This flat cylindrical protective cover, positioned above the cylindrical filter frame and with an inner diameter equal to that of the cylindrical filter frame, further buffers and guides the tissue fluid, ensuring it falls smoothly onto the cell filter membrane for a more uniform filtration effect.

[0016] Furthermore, the hemispherical diffuser block of the device is arranged in an upward arched manner below the conical flow guide and splash guard. When tissue fluid impacts the hemispherical diffuser block, its smooth outer wall can diffuse the tissue fluid in all directions, avoiding excessive local pressure that could damage the cell filtration membrane. This further optimizes the distribution of tissue fluid on the cell filtration membrane, improving filtration efficiency and quality. In addition, the entire flow guide and splash guard device has a simple structure and is easy to operate, providing a more stable and reliable environment for cell filtration. Attached Figure Description

[0017] Figure 1 This is a side-view three-dimensional structural diagram of an adjustable cell filter according to the present invention.

[0018] Figure 2 This is a frontal planar structural diagram of an adjustable cell filter according to the present invention.

[0019] Figure 3 This is a cross-sectional perspective view of the filter cover and its internal components of the present invention.

[0020] Figure 4 This is a schematic diagram of the external three-dimensional structure of the flow guiding and splash-proof device of this utility model.

[0021] Figure 5 This is a cross-sectional perspective view of the three-dimensional structure of the flow guiding and splash-proof device of this utility model.

[0022] In the diagram: 1. Base; 2. Test tube insertion hole; 3. Filter media collection test tube; 4. Connecting sleeve; 5. Filter cover; 6. Flow guiding and splash prevention device; 7. Vacuum tube; 8. Cylindrical filter rack; 9. Leakage hole; 10. Cell filter membrane; 11. Placement ring; 12. Baffle ring; 13. Handle; 14. Flat cylindrical protective cover; 15. Conical flow guiding and splash prevention cover; 16. Ring-type placement baffle; 17. Thin fixing rod; 18. Flat fixing block; 19. Hemispherical diffusion block. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] This utility model provides, for example Figures 1-5 An adjustable cell filter is shown, comprising a filter cover 5 and a connecting sleeve 4 disposed at the bottom of the filter cover 5. The top of the filter cover 5 is open, and a retaining ring 12 is fixed to the lower circular inner wall of the center of the filter cover 5. A placement ring 11 is placed at the top of the retaining ring 12, and a cylindrical filter frame 8 is fixed inside the placement ring 11. The top of the cylindrical filter frame 8 is open, and the bottom of the cylindrical filter frame 8 has equally spaced and densely distributed leakage holes 9. A cell filter membrane 10 is laid flat inside the bottom of the cylindrical filter frame 8, and the cell filter membrane 10 completely covers the top of the multiple leakage holes 9. The filter cover 5 serves as the starting container for the entire filtration process, and its top opening facilitates the pouring of the cell tissue fluid to be filtered. The retaining ring 12 on the lower side of the core is used to stabilize the placement ring 11, which in turn provides support for the cylindrical filter frame 8. The densely distributed leakage holes 9 at the bottom of the cylindrical filter frame 8 are the channels for the filtrate to flow out. The cell filter membrane 10, which is laid flat on the top of the leakage holes 9, is the key component for cell filtration. It can screen and filter cells and impurities of different sizes according to the pore size. The bottom third of the filter cover 5 is set in a conical shape. This conical design helps the liquid to be filtered to gather into the connecting sleeve 4. The filter cover 5 is connected to the inside of the filter media collection tube 3 through the connecting sleeve 4, so that the filtrate filtered by the cell filter membrane 10 can flow smoothly into the filter media collection tube 3 for collection.

[0025] like Figure 1 , Figure 2 and Figure 3As shown, a filter media collection tube 3 is inserted into the lower half of the connecting sleeve 4. A base 1 is installed on the outside of the bottom of the filter media collection tube 3. A test tube insertion hole 2 is installed inside the center of the base 1, and the filter media collection tube 3 is inserted into the test tube insertion hole 2. A vacuum tube 7 is installed on the upper side of the center of the right end of the connecting sleeve 4. The vacuum tube 7 is connected to an external vacuum pump through a connecting pipe. The vacuum tube 7 is an important part to realize the adjustable function. After the vacuum pump is turned on, a negative pressure environment can be formed in the filtration system. By adjusting the working intensity of the vacuum pump, the pressure in the filter cover 5 and the filter media collection tube 3 can be precisely controlled. When it is necessary to speed up the filtration, the suction power of the vacuum pump can be appropriately increased. The vacuum pump increases the negative pressure difference within the filtration system, allowing cell tissue fluid to pass through the cell filter membrane 10 more quickly, thus improving filtration efficiency. If the cells to be filtered are relatively fragile, the suction force of the vacuum pump can be reduced to decrease the negative pressure difference and allow the cells to complete the filtration process under a gentler pressure environment, ensuring cell activity and integrity. Multiple spare test tube sockets are also equidistantly arranged on the outer side and inner side of the center of the base 1, with different hole diameters. This allows for the adaptation of different specifications of filter media collection tubes 3, meeting the diverse requirements for filtrate collection volume and collection container specifications under different experimental needs, further enhancing the applicability and flexibility of the cell filter.

[0026] like Figure 1 , Figure 4 and Figure 5 As shown, a flow guiding and splash-proof device 6 is provided inside the upper side of the center of the filter cover 5. The flow guiding and splash-proof device 6 includes a handle piece 13, a flat cylindrical protective cover 14, a conical flow guiding and splash-proof cover 15, and an annular mounting baffle 16. The annular mounting baffle 16 is placed at the top of the filter cover 5. A handle piece 13 is provided on the outer wall of the right end of the annular mounting baffle 16. The conical flow guiding and splash-proof cover 15 is fixed to the inner wall of the annular ring of the annular mounting baffle 16. The cone tip of the conical flow guiding and splash-proof cover 15 faces downward, and the opening diameter at the top of the conical flow guiding and splash-proof cover 15 is larger than the opening diameter at the bottom. The conical flow guiding and splash-proof cover 15 is located inside the upper side of the center of the filter cover 5. The bottom end of the conical flow guiding and splash-proof cover 15 is connected to the flat cylindrical protective cover. 14. The bottom of the flat cylindrical protective cover 14 is open, and the ring-shaped baffle 16 is placed on the top of the filter cover 5 to provide stable support for the entire flow guiding and splash-proof device 6. The handle 13 facilitates the handling and placement of the device. When the cell tissue fluid to be filtered is poured in, it first comes into contact with the conical flow guiding and splash-proof cover 15. The cone tip of the cover faces downward, and the diameter of the top opening is larger than the diameter of the bottom opening. This special structure can guide the poured tissue fluid to flow slowly downward along the smooth inner wall, achieving initial convergence and flow guiding, and directing the liquid to the flat cylindrical protective cover 14 below, avoiding direct impact of the liquid on the cell filter membrane 10, and reducing membrane damage and uneven filtration problems that may be caused by impact.

[0027] likeFigure 1 , Figure 4 and Figure 5 As shown, the flow guiding and splash-proof device 6 also includes thin fixing rods 17, flat fixing blocks 18, and hemispherical diffuser blocks 19. A hemispherical diffuser block 19 is positioned directly below the bottom opening of the conical flow guiding and splash-proof cover 15, and is located at the center of the flat cylindrical protective cover 14. A flat fixing block 18 is positioned at the bottom of the hemispherical diffuser block 19. Thin fixing rods 17 are fixed to both ends of the flat fixing block 18, and the two thin fixing rods 17 are respectively fixed to the inner walls of the left and right ends of the flat cylindrical protective cover 14. The inner wall of the conical flow guiding and splash-proof cover 15 is smooth and burr-free. The flat cylindrical protective cover 14 is located above the cylindrical filter frame 8, and the inner diameter of the flat cylindrical protective cover 14 is equal to the inner diameter of the cylindrical filter frame 8. The flat cylindrical protective cover 14 can further buffer the tissue fluid, allowing the tissue fluid to enter the cylinder... The filter holder 8 allows for smoother flow, ensuring uniform distribution of tissue fluid on the cell filter membrane 10. This helps improve the uniformity and stability of filtration, guaranteeing the filtration effect. The hemispherical diffuser block 19 is positioned below the conical flow guide splash shield 15, arching upwards. The outer wall of the hemispherical diffuser block 19 is smooth and burr-free. The diameter of the hemispherical diffuser block 19 is smaller than the diameter of the bottom opening of the conical flow guide splash shield 15. When tissue fluid flows out from the bottom of the conical flow guide splash shield 15 and impacts the hemispherical diffuser block 19, its smooth and burr-free outer wall causes the tissue fluid to diffuse in all directions. This process effectively avoids excessive local pressure that could damage the cell filter membrane 10, further optimizing the distribution of tissue fluid on the cell filter membrane 10, making the filtration process more efficient and stable, thereby improving the overall quality of cell filtration.

[0028] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An adjustable cell filter, comprising a filter cover (5) and a connecting sleeve (4) disposed at the bottom end of the filter cover (5), wherein the top end of the filter cover (5) is open, a retaining ring (12) is fixed to the lower circular inner wall at the center of the filter cover (5), a placement ring (11) is placed at the top end of the retaining ring (12), and a cylindrical filter frame (8) is fixed inside the placement ring (11), wherein the top end of the cylindrical filter frame (8) is open, characterized in that: The bottom of the cylindrical filter frame (8) is densely covered with leakage holes (9) at equal intervals. The bottom of the cylindrical filter frame (8) is covered with a cell filter membrane (10), and the cell filter membrane (10) completely covers the top of the multiple leakage holes (9). The filter cover (5) is provided with a flow guiding and splash-proof device (6) on the upper side of the center. The flow guiding and splash-proof device (6) includes a handle (13), a flat cylindrical protective cover (14), a conical flow guiding and splash-proof cover (15), and an annular mounting baffle (16). The annular mounting baffle (16) is placed at the top of the filter cover (5). The outer wall of the right end of the annular mounting baffle (16) is provided with a handle (13). The annular inner wall of the annular mounting baffle (16) is fixed with a conical flow guiding and splash-proof cover (15). The cone tip of the conical flow guiding and splash-proof cover (15) faces downward, and the opening diameter at the top of the conical flow guiding and splash-proof cover (15) is larger than the opening diameter at the bottom. The conical flow guiding and splash-proof cover (15) is located inside the upper side of the center of the filter cover (5). The bottom end of the conical flow guiding and splash-proof cover (15) is connected to a flat cylindrical protective cover (14). The bottom end of the flat cylindrical protective cover (14) is set with an opening.

2. An adjustable cell filter according to claim 1, characterized in that: The flow guiding and splash-proof device (6) also includes a thin fixing rod (17), a flat fixing block (18), and a hemispherical diffuser block (19). The hemispherical diffuser block (19) is located directly below the bottom opening of the conical flow guiding and splash-proof cover (15), and the hemispherical diffuser block (19) is located at the center inside the flat cylindrical protective cover (14). The bottom end of the hemispherical diffuser block (19) is provided with a flat fixing block (18). The flat fixing block (18) is fixed with thin fixing rods (17) at both ends, and the two thin fixing rods (17) are respectively fixed to the inner walls of the left and right ends of the flat cylindrical protective cover (14).

3. An adjustable cell filter according to claim 2, characterized in that: The inner wall of the conical flow guide splash shield (15) is smooth and burr-free. The flat cylindrical protective shield (14) is located above the cylindrical filter frame (8), and the inner diameter of the flat cylindrical protective shield (14) is equal to the inner diameter of the cylindrical filter frame (8).

4. An adjustable cell filter according to claim 2, characterized in that: The hemispherical diffuser block (19) is arranged in an upward arched manner below the conical flow guide splash shield (15), and the outer wall of the hemispherical diffuser block (19) is smooth and burr-free. The diameter of the hemispherical diffuser block (19) is smaller than the diameter of the bottom opening of the conical flow guide splash shield (15).

5. An adjustable cell filter according to claim 1, characterized in that: The lower half of the connecting sleeve (4) is fitted with a filter material collection test tube (3). The bottom of the filter material collection test tube (3) is provided with a base (1). The center of the base (1) is provided with a test tube insertion hole (2), and the filter material collection test tube (3) is inserted into the test tube insertion hole (2).

6. An adjustable cell filter according to claim 5, characterized in that: The bottom third of the filter cover (5) is tapered. The filter cover (5) is connected to the inside of the filter material collection tube (3) through the connecting sleeve (4). A vacuum tube (7) is provided on the upper side of the center of the right end of the connecting sleeve (4).

7. An adjustable cell filter according to claim 6, characterized in that: The vacuum tube (7) is connected to an external vacuum pump through a connecting tube. Multiple test tube spare holes are also equidistantly arranged on the outer side of the center of the base (1), and the diameters of the multiple test tube spare holes are different.

Images