A multi-well cell culture plate

Abstract

The utility model relates to a kind of porous cell culture plate, including culture plate component, culture plate component includes culture plate, there are several groups of PCR manifold in the bottom of culture plate along left and right direction evenly, and the first connecting part and the second connecting part are detachably mounted together between the two groups of PCR manifold adjacent to left and right, each group of PCR manifold includes several PCR tubes distributed along front and back direction;Support component is provided below culture plate.The utility model is placed in the support component below culture plate component, ensure that culture plate is in horizontal state after being placed, prevent culture plate from shaking left and right, can provide stable support and accurate positioning function.

Description

Technical Field

[0001] This utility model relates to the field of biological instrument technology, and in particular to a porous cell culture plate. Background Technology

[0002] A search revealed Chinese patent publication number CN221421098U, which discloses a PCR tube for rapid PCR. The technical solution includes: a bracket on which a PCR tube is placed; a PCR tube cap is installed on the top of the PCR tube; an aluminum-plastic composite film is adhered to the bottom of the PCR tube; the bottom circumference of the PCR tube cap has an outwardly protruding arc-shaped step; PCR tube placement holes are evenly spaced on the bracket; PCR tubes are installed on the inner wall of the PCR tube placement holes; vertically marked letters are located on the top left end of the bracket; and horizontally marked numbers are located on the top rear end of the bracket.

[0003] Existing multi-well cell culture plates lack dedicated support components. PCR plates rely primarily on their own structure for support during placement. When supporting multiple PCR tubes and reaction solutions, instability can cause them to tilt, affecting the accuracy of experimental procedures. Furthermore, while adjacent sets of PCR tubes can be detachably connected via a docking structure, the lack of a clear guide means manual alignment is required during installation. Inaccurate alignment can lead to installation difficulties or even damage to the docking structure.

[0004] In view of the above-mentioned shortcomings, the designer has actively researched and innovated in order to create a porous cell culture plate that has greater industrial application value. Utility Model Content

[0005] To solve the above-mentioned technical problems, the purpose of this utility model is to provide a porous cell culture plate.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A multi-well cell culture plate includes a culture plate assembly, the culture plate assembly includes a culture plate, and several sets of PCR tubes are evenly distributed along the left-right direction at the bottom of the culture plate. Two adjacent sets of PCR tubes are detachably connected together through a first connecting part and a second connecting part. Each set of PCR tubes includes several PCR tubes distributed along the front-back direction.

[0008] A support assembly is provided below the culture plate. The support assembly includes a base and side frames located on the left and right sides of the base. An intermediate frame is installed on the inner side between the two side frames. Several through holes for PCR tubes are opened on the intermediate frame. A positioning groove with a downward indentation is opened on the top of the side frame. Support blocks are provided on the left and right sides of the bottom of the culture plate. The positioning blocks at the bottom of the support blocks can be embedded into the positioning groove below from top to bottom.

[0009] The first connecting part includes a docking groove formed in the culture plate along the front-back direction, and a guide groove formed on the outside of the docking groove; the second connecting part includes a guide block arranged on the culture plate along the front-back direction, and a docking block arranged on the outside of the guide block.

[0010] After the guide block is inserted into the guide groove in the front-to-back direction, the docking block is installed in the docking groove. The first magnetic attraction structure located in the docking groove is compatible with the second magnetic attraction structure located on the docking block.

[0011] As a further improvement of this utility model, support feet are installed on both the left and right sides of the bottom of the base.

[0012] As a further improvement of this invention, the PCR loop at the top of the PCR tube is located on the top outer side of the culture plate.

[0013] As a further improvement of this invention, side plates are installed on the culture plates on both the front and back sides of each PCR tube.

[0014] As a further improvement of this utility model, the side plate is inclined outward relative to the inner culture plate.

[0015] As a further improvement of this utility model, a card plate is rotatably installed on the culture plate inside the docking groove through a rotating structure, and the buckling protrusion on the card plate can be snapped into the buckling groove on the docking block.

[0016] As a further improvement of this utility model, a conical groove is provided on the outside of the guide groove, and a conical block adapted to the conical groove is provided on the guide block.

[0017] As a further improvement of this invention, each PCR tube set includes eight PCR tubes.

[0018] By means of the above solution, this utility model has at least the following advantages:

[0019] This invention uses a support assembly located below the culture plate assembly to ensure that the culture plate is placed horizontally, preventing the culture plate from swaying left and right, and providing stable support and precise positioning.

[0020] The first magnetic attraction structure of this utility model is compatible with the second magnetic attraction structure. During installation, when the guide block is inserted into the guide groove, the magnetic attraction can automatically align the docking block and the docking groove, eliminating the need for manual precise alignment and greatly improving installation efficiency.

[0021] In this invention, the side plates on both sides of each PCR tube are tilted outward relative to the inner culture plate, making it easier for operators to handle the culture plate. They also serve as guides during assembly, facilitating the alignment of adjacent culture plates.

[0022] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the following are the preferred embodiments of this utility model and are described in detail with reference to the accompanying drawings. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the structure of a porous cell culture plate according to the present invention;

[0025] Figure 2 yes Figure 1 A schematic diagram of the split structure;

[0026] Figure 3 yes Figure 2 Schematic diagram of the middle support assembly;

[0027] Figure 4 yes Figure 2 A schematic diagram of the structure of the culture plate assembly;

[0028] Figure 5 yes Figure 4 A schematic diagram showing the disassembly structure of two adjacent PCR tubes;

[0029] Figure 6 yes Figure 5 A partially enlarged structural diagram of the first connecting part and the second connecting part;

[0030] Figure 7 yes Figure 4 A schematic diagram of the installation structure of two adjacent PCR tubes;

[0031] Figure 8 yes Figure 7 A partially enlarged structural diagram of the first and second connecting parts.

[0032] The meanings of the labels in the figures are as follows.

[0033] Scaffold assembly 1, culture plate assembly 2;

[0034] 11. Base; 12. Side frame; 13. Middle frame; 14. Positioning groove; 15. Support leg;

[0035] Culture plate 21, PCR clamping ring 22, support block 23, positioning block 24, side plate 25, PCR tube 26, first connecting part 27, second connecting part 28;

[0036] 271. Connecting groove, 272. First magnetic attraction structure, 273. Guide groove, 274. Conical groove, 275. Clamping plate, 276. Rotating structure, 277. Snap-on protrusion;

[0037] Connecting block 281, guide block 282, snap-fit ​​groove 283, second magnetic attraction structure 284, conical block 285. Detailed Implementation

[0038] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0039] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0040] The first embodiment of this utility model:

[0041] like Figures 1-2 As shown, a porous cell culture plate of this embodiment mainly includes a scaffold assembly 1 and a culture plate assembly 2. The scaffold assembly 1 is located below the culture plate assembly 2 and is used to support the bottom of the culture plate assembly 2.

[0042] I. Culture plate assembly 2:

[0043] like Figure 4 The culture plate assembly 2 mainly includes a culture plate 21. Several sets of PCR tubes are evenly distributed along the left-right direction at the bottom of the culture plate 21. Each set of PCR tubes includes eight PCR tubes 26. Two adjacent sets of PCR tubes are detachably installed together through a first connecting part 27 and a second connecting part 28. Each set of PCR tubes includes several PCR tubes 26 distributed along the front-back direction.

[0044] Support blocks 23 are provided on both the left and right sides of the bottom of the culture plate 21. The positioning block 24 at the bottom of the support block 23 can be embedded into the positioning groove 14 below from top to bottom.

[0045] like Figures 5-8 As shown:

[0046] The first connecting part 27 includes a docking groove 271 opened in the culture plate 21 along the front-back direction, a guide groove 273 opened on the outside of the docking groove 271, and a first magnetic attraction structure 272 (which can be an N52 grade neodymium iron boron permanent magnet array) is embedded on the upper and lower sides of the docking groove 271 respectively.

[0047] The second connecting part 28 includes a guide block 282 disposed on the culture plate 21 along the front-back direction, a docking block 281 disposed on the outside of the guide block 282, and a second magnetic attraction structure 284 (a soft iron magnetic attraction plate with a nickel-plated surface for rust prevention) is embedded on the upper and lower sides of the docking block 281 respectively.

[0048] The mounting structure of the first connecting part 27 and the second connecting part 28:

[0049] After the guide block 282 is inserted into the guide groove 273 in the front-back direction, the docking block 281 is installed in the docking groove 271. The first magnetic attraction structure 272 located in the docking groove 271 is adapted to the second magnetic attraction structure 284 located on the docking block 281.

[0050] A tapered groove 274 is provided on the outer side of the guide groove 273, and a tapered block 285 adapted to the tapered groove 274 is provided on the guide block 282.

[0051] The guide groove is opened in the culture plate along the front-to-back direction, and its length is the same as that of the guide block. After the guide block is inserted into the guide groove, it can restrict the left and right movement of the culture plate and ensure that the docking block enters the docking groove accurately.

[0052] The tapered groove on the outside of the guide groove matches the tapered block on the guide block. When inserted, the tapered structure can automatically guide the guide block into the guide groove. Even if there is a certain angular deviation, it can be aligned smoothly, reducing the difficulty of installation and improving the speed and accuracy of installation.

[0053] The magnetic pole distribution of the first and second magnetic attraction structures is fixed. If installed in reverse, the magnetic attraction will repel each other, preventing proper installation and thus serving as a foolproof method. Simultaneously, the shape design of the mating block and mating groove (e.g., the mating block has a magnetic attraction structure only on one side) also prevents reverse installation.

[0054] The first magnetic attraction structure is compatible with the second magnetic attraction structure. During installation, as the guide block is inserted into the guide groove, the magnetic attraction can automatically align the docking block and the docking groove, eliminating the need for manual precise alignment and greatly improving installation efficiency.

[0055] The magnetic structure, composed of the first magnetic structure 272 and the second magnetic structure 284, enables "instant bonding", shortening the PCR tube splicing time.

[0056] A clamping plate 275 is rotatably mounted on the culture plate 21 inside the docking groove 271 via a rotating structure 276. The buckling protrusion 277 on the clamping plate 275 can be snapped into the buckling groove 283 on the docking block 281.

[0057] The plate rotates via a rotating structure, and the latching protrusions latch into the latching grooves of the mating block, forming a mechanical lock to prevent the connection from loosening due to vibration or other factors, thus ensuring structural stability.

[0058] The rotating structure inside the docking groove can be a pivot or hinge, around which the locking plate rotates. The shape of the locking protrusion matches the shape of the locking groove (such as trapezoidal or arc shape, etc.) to ensure a tight lock.

[0059] The PCR upper retaining ring 22 at the top of the PCR tube 26 is located on the top outer side of the culture plate 21. The PCR upper retaining ring 22 and the PCR tube 26 are integrally formed with the culture plate 21.

[0060] Furthermore, the PCR upper retaining ring 22 and the PCR tube 26 can be separate from the culture plate 21. That is, the PCR upper retaining ring at the top of the PCR tube is located on the outer side of the top of the culture plate, which can prevent the PCR tube from falling off the bottom of the culture plate, and at the same time provide an upward limit for the PCR tube, ensuring that the PCR tube is fixed in position within the central through-hole. The inner diameter of the PCR upper retaining ring is interference-fitted with the outer diameter of the PCR tube, and is tightly fixed after installation.

[0061] The side plates on both sides of each PCR tube are tilted outwards relative to the inner culture plate, making it easier for operators to handle the culture plate and also serving as a guide during splicing to facilitate the alignment of adjacent culture plates. The tilt angle of the side plates is 10°~15°, which does not affect the strength of the culture plate while providing a good grip.

[0062] II. Support Component 1:

[0063] like Figure 3 The support assembly 1 mainly includes a base 11, a side frame 12, and a middle frame 13.

[0064] The base 11 is a rectangular plate, the side frames 12 are vertically fixed to the left and right sides of the base 11, and the middle frame 13 is fixed between the two side frames 12 by bolts or welding. The diameter of the middle through hole is slightly larger than the outer diameter of the PCR tube, leaving a certain gap for easy insertion and removal.

[0065] A downward-facing positioning groove 14 is provided on the top of the side frame 12. The positioning groove 14 is a rectangular groove with a depth of 5-10mm. The positioning block 24 is a rectangular block that matches the shape of the positioning groove 14.

[0066] The positioning block at the bottom of the culture plate's support block is embedded from top to bottom into the positioning groove at the top of the side frame, forming a precise vertical position and preventing the culture plate from wobbling from side to side. The depth of the positioning groove and the height of the positioning block are matched to ensure that the culture plate is in a horizontal position after placement, improving stability.

[0067] The central through-holes on the intermediate frame correspond to the number and position of the PCR tubes. The PCR tubes pass through the central through-holes, and the intermediate frame supports and constrains the PCR tubes, preventing them from wobbling back and forth. It also prevents the PCR tubes from tilting due to uneven force, ensuring the stability of the sample reaction environment.

[0068] Support legs 15 are installed on both the left and right sides of the base 11. These legs raise the height of the support assembly from the ground, preventing the base from directly contacting the tabletop and becoming damp. They also increase the contact area between the base and the tabletop, improving overall stability. The legs can be made of rubber with anti-slip textured bottoms and a height of 5-10mm.

[0069] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0070] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0071] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A multi-well cell culture plate, comprising a culture plate assembly (2), the culture plate assembly (2) comprising a culture plate (21), wherein a plurality of PCR tubes are evenly distributed along the left-right direction at the bottom of the culture plate (21), and two adjacent sets of PCR tubes are detachably mounted together through a first connecting part (27) and a second connecting part (28), and each set of PCR tubes comprises a plurality of PCR tubes (26) distributed along the front-back direction. Its features are: A support assembly (1) is provided below the culture plate (21). The support assembly (1) includes a base (11) and side frames (12) located on the left and right sides of the base (11). An intermediate frame (13) is installed on the inner side between the two side frames (12). Several intermediate through holes for PCR tubes (26) to pass through are provided on the intermediate frame (13). A positioning groove (14) with a downward indentation is provided on the top of the side frame (12). Support blocks (23) are provided on the left and right sides of the bottom of the culture plate (21). The positioning block (24) at the bottom of the support block (23) can be embedded into the positioning groove (14) below from top to bottom. The first connecting part (27) includes a docking groove (271) opened in the culture plate (21) along the front-back direction, and a guide groove (273) is opened on the outside of the docking groove (271). The second connecting part (28) includes a guide block (282) arranged in the culture plate (21) along the front-back direction, and a docking block (281) is arranged on the outside of the guide block (282). After the guide block (282) is inserted into the guide groove (273) in the front-back direction, the docking block (281) is installed in the docking groove (271). The first magnetic attraction structure (272) located in the docking groove (271) is adapted to the second magnetic attraction structure (284) located on the docking block (281).

2. The porous cell culture plate as described in claim 1, characterized in that, Support legs (15) are installed on both the left and right sides of the bottom of the base (11).

3. The porous cell culture plate as described in claim 1, characterized in that, The PCR loop (22) at the top of the PCR tube (26) is located on the top outer side of the culture plate (21).

4. A porous cell culture plate as described in claim 1, characterized in that, Side plates (25) are installed on the culture plates (21) on both sides of each group of PCR tubes.

5. A porous cell culture plate as described in claim 4, characterized in that, The side plate (25) is inclined outward relative to the inner culture plate (21).

6. A porous cell culture plate as described in claim 1, characterized in that, A card plate (275) is rotatably mounted on the culture plate (21) inside the docking groove (271) via a rotating structure (276). The buckling protrusion (277) on the card plate (275) can be snapped into the buckling groove (283) on the docking block (281).

7. A porous cell culture plate as described in claim 1, characterized in that, A tapered groove (274) is provided on the outside of the guide groove (273), and a tapered block (285) adapted to the tapered groove (274) is provided on the guide block (282).

8. A porous cell culture plate as described in claim 1, characterized in that, Each PCR bundle consists of eight PCR tubes (26).

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