Water quality microorganism flow cytometry fluorescence labeling device
By designing a flow cytometry fluorescent labeling device with a protective cover, fluorescent labeling components, and a light-transmitting inner cavity, the problems of non-specific binding and uneven labeling efficiency in water quality microbial cell fluorescent labeling devices were solved, achieving precise fluorescent labeling and target recognition.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- BEIJING SCIENCE & TECHNOLOGY INSPECTION & TESTING TECHNOLOGY CO LTD
- Filing Date
- 2025-07-22
- Publication Date
- 2026-06-09
AI Technical Summary
Existing fluorescent labeling devices for water microbial cells suffer from background interference due to non-specific binding, uneven labeling efficiency, and target recognition bias, especially for low-abundance microorganisms, which are prone to missed detection.
A flow cytometry fluorescent labeling device was designed, comprising a protective cover, a fluorescent labeling component, and a light-transmitting inner cavity. The sample is transported by a conveyor belt, and a stepper motor controls the rotating baffle and the inner positioning carrier to allow microbial cells to pass through the light-transmitting inner cavity one by one. Combined with the fluorescent labeling component and a fluorescence microscope, precise fluorescent labeling is achieved, and signal data is monitored and recorded in real time.
It achieves precision and consistency in fluorescent labeling of microorganisms in water quality, reduces non-specific binding and target recognition bias, and ensures accurate labeling of low-abundance microorganisms.
Smart Images
Figure CN224341391U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cell fluorescence labeling technology and relates to a flow cytometry cell fluorescence labeling device for aquatic microorganisms. Background Technology
[0002] The low accuracy of existing fluorescent labeling of aquatic microorganisms mainly stems from three aspects: background interference caused by non-specific binding, uneven labeling efficiency, and target recognition bias. Uneven labeling efficiency is caused by differences in microbial cell membrane structure, physiological state, and dye permeability. Gram-positive bacteria, due to their thick peptidoglycan layer, may hinder the entry of fluorescent probes, while dormant microorganisms have low metabolic activity and a weakened response to ATP- or enzyme-dependent fluorescent labeling. For example, when labeling live cells with CFDA-SE, the fluorescence intensity of dormant bacteria is significantly lower than that of active bacteria, leading to quantitative bias in the target bacterial population. Conventional solutions include optimizing staining concentration and incubation time to reduce non-specific binding, such as determining the minimum effective dye concentration through preliminary experiments, and removing free dye and background impurities through centrifugation and washing. However, optimizing staining parameters may result in insufficient labeling of some target bacteria, especially low-abundance microorganisms, which can easily lead to missed detections. Therefore, there is an urgent need for a flow cytometry fluorescent labeling device for aquatic microorganisms to solve these problems. Utility Model Content
[0003] To address the shortcomings of existing technologies, the purpose of this invention is to provide a flow cytometry fluorescent labeling device for aquatic microorganisms, thereby resolving the problems mentioned in the background section.
[0004] This utility model is achieved through the following technical solution: a flow cytometry fluorescent labeling device for aquatic microorganisms, comprising: a protective cover, a fluorescent labeling component and a light-transmitting inner cavity, wherein the lower end of the protective cover is provided with a set of support frames for supporting the lower end thereto, and the lower end inside the protective cover is provided with a set of conveyor belts for batch transporting aquatic microbial fluid cells.
[0005] The conveyor belt is located on the inner side of the upper end of the support frame. The upper part of the middle position of the support frame is provided with an inner positioning frame for marking and positioning water microbial fluid cells. The lower end of the inner positioning frame is provided with a set of stepper motors for positioning, blocking and stepping release, as well as a rotating stop bar controlled by the stepper motors. The middle position of the upper end of the inner positioning frame is provided with a set of light-transmitting inner cavities for specifying the position of fluorescent markers.
[0006] In a preferred embodiment, the upper part of the middle position of the protective cover is provided with a set of viewing ports for facilitating fluorescent labeling by the fluorescent labeling component. The viewing ports have the same cross-sectional length and width as the light-transmitting inner cavity and are completely overlapped in the vertical direction. In actual use, the device is first pushed to the rear of the protective cover by the moving wheels. After ensuring that the support frame is placed stably, the water sample to be tested is placed into the sample inlet of the conveyor belt. The conveyor belt, under the control of the stepper motor, transports the sample in batches to the inner positioning frame area. The rotating baffle releases the sample intermittently according to the preset program, so that the microbial fluid cells pass through the light-transmitting inner cavity one by one. The fluorescent labeling component accurately irradiates the cells through the perfectly aligned viewing ports and the light-transmitting inner cavity, exciting the fluorescent signal and completing the labeling process.
[0007] In a preferred embodiment, the upper end of the fluoroscopic port is provided with a set of fluorescent labeling components for fluorescently labeling aquatic microbial fluid cells. The fluorescent labeling components include an adjusting screw and a base, and the outer side of the upper end of the adjusting screw is provided with a set of guide support seats for moving and guiding it.
[0008] In a preferred embodiment, a set of guide posts are provided on the front and rear sides of the lower end of the guide support for guiding the movement of the movable block. A set of movable blocks is provided in the middle of the two sets of guide posts and the adjusting screw for driving the fluorescent marker, laser generator and fluorescence microscope to move synchronously.
[0009] In a preferred embodiment, the right side of the movable block is provided with a set of carrier plates for connecting and fixing with the fluorescent labeler, and the front side of the carrier plate is provided with a set of laser generators for fluorescent labeling of aquatic microbial fluid cells and a fluorescence microscope for observing the fluorescent labeling.
[0010] In a preferred embodiment, the lower ends of the two sets of guide columns are provided with a set of bases for maintaining stability, the laser generator marking end is located directly above the fluoroscopic opening, and the lower end of the fluorescent marking component is provided with a set of mounting platforms for supporting it.
[0011] In a preferred embodiment, the upper right side of the mounting platform is equipped with a set of control devices for controlling the fluorescent labeling components. The control devices are computer hosts with control software for the fluorescent labeling components and related equipment modules. The lower end of the mounting platform is equipped with a set of storage cabinets for placing related consumables. The lower end of the storage cabinets is equipped with several sets of casters for easy movement. In actual use, after the control device is turned on and the system is initialized, the water sample to be tested is placed at the sample inlet of the conveyor belt. The conveyor belt, driven by a stepper motor, transports the sample to the inner positioning frame area. The rotating baffle sorts the sample according to the program, so that the microbial cells accurately pass through the overlapping area of the light-transmitting inner cavity and the fluoroscopic port. The operator controls the three-dimensional movement of the movable block by adjusting the screw, which drives the laser generator on the carrier plate to align with the fluoroscopic port for fluorescence excitation. At the same time, the fluorescence microscope monitors the labeling effect in real time. During the labeling process, the guide column and guide support seat can ensure the movement accuracy of the optical components. The control device records the fluorescence signal data synchronously to ensure accurate fluorescent labeling.
[0012] After adopting the above technical solution, the beneficial effects of this utility model are as follows: First, the device is pushed to the back of the protective cover by the moving wheels. After ensuring that the support frame is placed stably, the water quality sample to be tested is put into the sample inlet of the conveyor belt. Under the control of the stepper motor, the conveyor belt transports the sample to the inner positioning frame area in batches. The rotating baffle releases the sample intermittently according to the preset program, so that the microbial fluid cells pass through the light-transmitting inner cavity one by one. The fluorescent labeling component accurately irradiates the cells through the fully aligned fluoroscopic port and the light-transmitting inner cavity, excites the fluorescent signal and completes the labeling process.
[0013] After the control equipment is activated and the system is initialized, the water sample to be tested is placed at the sample inlet of the conveyor belt. Driven by a stepper motor, the conveyor belt transports the sample to the inner positioning carrier area. The rotating baffle sorts the sample according to the program, so that the microbial cells can accurately pass through the overlapping area of the light-transmitting inner cavity and the fluoroscopic port. The operator controls the three-dimensional movement of the movable block by adjusting the screw, which drives the laser generator on the carrier plate to align with the fluoroscopic port for fluorescence excitation. At the same time, the fluorescence microscope monitors the labeling effect in real time. During the labeling process, the guide column and guide support can ensure the movement accuracy of the optical components. The control equipment records the fluorescence signal data synchronously to ensure accurate fluorescence labeling. Attached Figure Description
[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0015] Figure 1This is a right oblique front view schematic diagram of the flow cytometry fluorescent labeling device for aquatic microorganisms according to the present invention;
[0016] Figure 2 This is a top view of the fluorescent labeling component and the storage cabinet in a flow cytometry fluorescent labeling device for water microorganisms according to this utility model.
[0017] Figure 3 This is a schematic diagram of the right oblique front view of the internal positioning carrier structure in the flow cytometry fluorescent labeling device for aquatic microorganisms of this utility model.
[0018] Figure 4 This is a right-side view of the internal structure of the fluorescent labeling component in a flow cytometry fluorescent labeling device for water microorganisms according to this utility model.
[0019] In the diagram: 100-protective cover, 110-support frame, 120-conveyor belt, 130-moving cabinet, 140-control equipment, 150-fluorescent marking component, 160-viewing port, 170-mounting platform, 180-storage cabinet, 190-moving wheels, 200-inner positioning frame, 210-light-transmitting inner cavity;
[0020] 15a-Adjusting screw, 15b-Guide support, 15c-Modible block, 15d-Fluorescent marker, 15e-Laser generator, 15f-Fluorescent microscope, 15g-Base. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0022] Please see Figures 1-4 As the first embodiment of this utility model: a flow cytometry fluorescent labeling device for aquatic microorganisms, comprising: a protective cover 100, a fluorescent labeling component 150 and a light-transmitting inner cavity 210, the lower end of the protective cover 100 is provided with a set of support frames 110 for supporting the lower end thereto, and the lower end of the inner side of the protective cover 100 is provided with a set of conveyor belts 120 for batch conveying aquatic microbial fluid cells.
[0023] The conveyor belt 120 is installed on the inner side of the upper end of the support frame 110. The upper end of the middle position of the support frame 110 is provided with an inner positioning carrier 200 for marking and positioning water microbial fluid cells. The lower end of the inner positioning carrier 200 is provided with a set of stepper motors for positioning, blocking and stepping release, as well as a rotating stop bar controlled by the stepper motors. The middle position of the upper end of the inner positioning carrier 200 is provided with a set of light-transmitting inner cavities 210 for specifying the position of fluorescent markers.
[0024] The upper part of the middle position of the protective cover 100 is provided with a set of transparent openings 160 for the fluorescent labeling component 150 to perform fluorescent labeling. The transparent openings 160 and the light-transmitting inner cavity 210 have the same cross-sectional length and width and are completely overlapped in the vertical direction. In actual use, the device is first pushed to the rear of the protective cover 100 by the moving wheels 190. After ensuring that the support frame 110 is placed stably, the water quality sample to be tested is put into the sample inlet of the conveyor belt 120. Under the control of the stepper motor, the conveyor belt 120 transports the sample in batches to the inner positioning carrier 200 area. The rotating baffle releases the sample intermittently according to the preset program, so that the microbial fluid cells pass through the light-transmitting inner cavity 210 one by one. The fluorescent labeling component 150 accurately irradiates the cells through the perfectly aligned transparent openings 160 and the light-transmitting inner cavity 210, exciting the fluorescent signal and completing the labeling process.
[0025] Please see Figures 1-4 As a second embodiment of this utility model: based on the description in the above embodiments, further, the upper end of the fluoroscopic port 160 is provided with a set of fluorescent labeling components 150 for fluorescent labeling of water microbial fluid cells. The fluorescent labeling component 150 includes an adjusting screw 15a and a base 15g. The outer side of the upper end of the adjusting screw 15a is provided with a set of guide support seats 15b for moving and guiding it.
[0026] The lower end of the guide support 15b is provided with a set of guide posts on the front and rear sides for moving and guiding the movable block 15c. The two sets of guide posts and the adjusting screw 15a are located in the middle of the movable block 15c for driving the fluorescent marker 15d, the laser generator 15e and the fluorescence microscope 15f to move synchronously.
[0027] On the right side of the movable block 15c is a set of carrier plates for connecting and fixing with the fluorescent labeler 15d. On the front side of the carrier plate is a set of laser generator 15e for fluorescent labeling of aquatic microbial fluid cells and a fluorescence microscope 15f for observing the fluorescent labeling.
[0028] The lower ends of the two sets of guide columns are provided with a set of bases 15g for maintaining stability. The laser generator 15e marking end is located directly above the viewing port 160. The lower end of the fluorescent marking component 150 is provided with a set of mounting platforms 170 for supporting it.
[0029] The upper right side of the mounting platform 170 is equipped with a control device 140 for controlling the fluorescent labeling component 150. The control device 140 is a computer host with control software for the fluorescent labeling component 150 and related equipment modules. The lower part of the mounting platform 170 is equipped with a storage cabinet 180 for placing related consumables. The lower part of the storage cabinet 180 is equipped with several sets of casters 190 for easy movement. In actual use, after the control device 140 is turned on and the system is initialized, the water sample to be tested is placed at the sample inlet of the conveyor belt 120. The conveyor belt 120 transports the sample under the drive of a stepper motor. The sample is positioned in the inner positioning carrier 200 area. The rotating baffle sorts the sample according to the program, so that the microbial cells can accurately pass through the overlapping area of the light-transmitting inner cavity 210 and the fluoroscopic port 160. The operator controls the three-dimensional movement of the movable block 15c by adjusting the screw 15a, which drives the laser generator 15e on the carrier plate to align with the fluoroscopic port 160 for fluorescence excitation. At the same time, the fluorescence microscope 15f monitors the labeling effect in real time. During the labeling process, the guide column and guide support 15b can ensure the movement accuracy of the optical components. The control device 140 records the fluorescence signal data synchronously to ensure accurate fluorescence labeling.
[0030] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A flow cytometry fluorescent labeling device for aquatic microorganisms, comprising: The protective cover (100), fluorescent marking component (150), and light-transmitting inner cavity (210) are characterized in that: the lower end of the protective cover (100) is provided with a set of support frame (110) for supporting its lower end, and the lower end of the inner side of the protective cover (100) is provided with a set of conveyor belt (120) for batch conveying water quality microbial fluid cells. The conveyor belt (120) is located on the inner side of the upper end of the support frame (110). The upper end of the middle position of the support frame (110) is provided with an inner positioning carrier (200) for marking and positioning water microbial fluid cells. The lower end of the inner positioning carrier (200) is provided with a set of stepper motors for positioning and blocking and stepping release, as well as a rotating stop bar controlled by the stepper motors. The middle position of the upper end of the inner positioning carrier (200) is provided with a set of light-transmitting inner cavities (210) for specifying the position of fluorescent markers.
2. The flow cytometry fluorescent labeling device for aquatic microorganisms according to claim 1, characterized in that: The upper part of the middle position of the protective cover (100) is provided with a set of transparent openings (160) for the fluorescent marking component (150) to perform fluorescent marking. The transparent openings (160) and the light-transmitting inner cavity (210) have the same cross-sectional length and width, and are completely overlapped in the vertical direction.
3. The flow cytometry fluorescent labeling device for aquatic microorganisms according to claim 2, characterized in that: The upper end of the fluoroscopic port (160) is provided with a set of fluorescent labeling components (150) for fluorescent labeling of aquatic microbial fluid cells. The fluorescent labeling component (150) includes an adjusting screw (15a) and a base (15g). The outer side of the upper end of the adjusting screw (15a) is provided with a set of guide support seats (15b) for moving and guiding it.
4. The flow cytometry fluorescent labeling device for aquatic microorganisms according to claim 3, characterized in that: The guide support base (15b) has a set of guide posts on the front and rear sides at the lower end for guiding the movement of the movable block (15c). The two sets of guide posts and the adjusting screw (15a) are connected in the middle of the movable block (15c) for driving the fluorescent marker (15d), laser generator (15e) and fluorescence microscope (15f) to move synchronously.
5. The flow cytometry fluorescent labeling device for aquatic microorganisms according to claim 4, characterized in that: The right side of the movable block (15c) is provided with a set of carrier plates for connecting and fixing with the fluorescent labeler (15d). The front side of the carrier plate is provided with a set of laser generators (15e) for fluorescent labeling of aquatic microbial fluid cells and a fluorescence microscope (15f) for observing the fluorescent labeling.
6. The flow cytometry fluorescent labeling device for aquatic microorganisms according to claim 4, characterized in that: The lower ends of the two sets of guide columns are provided with a set of bases (15g) for maintaining stability. The marking end of the laser generator (15e) is located directly above the viewing port (160). The lower end of the fluorescent marking component (150) is provided with a set of mounting platforms (170) for supporting it.
7. The flow cytometry fluorescent labeling device for aquatic microorganisms according to claim 6, characterized in that: The upper right side of the mounting platform (170) is provided with a set of control devices (140) for controlling the fluorescent marking component (150). The control device (140) is a computer host with control software for the fluorescent marking component (150) and related equipment modules. The lower end of the mounting platform (170) is provided with a set of storage cabinets (180) for placing related consumables. The lower end of the storage cabinets (180) is provided with several sets of movable wheels (190) for easy movement.