A grapevine leafroll disease leaf sampling device

Abstract

The application relates to a grape phylloxera leaf sampling device, which comprises a sampling assembly and a disinfecting assembly. The sampling assembly comprises a fixing plate, a pressure bearing plate, a sampling punch, a punch driving mechanism and a sampling tube. A positioning hole is arranged at one end of the fixing plate. The punch driving mechanism is arranged on the fixing plate and is in transmission cooperation with the sampling punch. The punch driving mechanism is used for driving the sampling punch to move in the axial direction of the positioning hole. The pressure bearing plate is arranged on the lower side of the fixing plate and keeps a leaf clamping gap with the fixing plate. A sampling hole is arranged at one end of the pressure bearing plate in the axial direction of the positioning hole. The sampling tube is detachably arranged in the sampling hole and has an upward opening. The disinfecting assembly comprises a disinfectant cavity arranged on the fixing plate and a spraying mechanism. The disinfectant cavity is in communication with the inlet of the spraying mechanism. The outlet of the spraying mechanism extends to the vicinity of the sampling punch and is used for spraying disinfectant towards the sampling punch, so that the grape phylloxera leaf can be continuously sampled after disinfection.

Description

Technical Field

[0001] This application belongs to the technical field of leaf sampling equipment, and specifically relates to a leaf sampling device for grape leaf curl disease. Background Technology

[0002] Grape leafroll is a viral disease that can spread to healthy grapevines not only through scions, cuttings, or dodder growing in the field, but also through human intervention during pruning, grafting, or sampling. This allows the virus to spread rapidly among grapevines and severely impact grape yield and quality. Because grape leafroll causes leaf curling, on-site leaf sampling is necessary to detect the causative virus and facilitate subsequent treatment. To prevent sample contamination or degradation, collected samples should be immediately placed in test tubes containing an RNA stabilizer to slow viral RNA degradation and improve detection sensitivity.

[0003] In the prior art, Chinese utility model publication CN217111575U discloses a blade sampler, including a first body and a second body, which are rotatably connected by a rotating shaft. The front ends of the first and second bodies are sampling mechanisms, and the rear ends of the first and second bodies are handles. A pressing plate is provided at the front end of the first body, and a support plate is provided at the front end of the second body. A pressing element is provided between the pressing plate and the support plate. The pressing element includes a punching post and a spring sleeved on the punching post. A through hole is provided on the support plate for the punching post to pass through. A blade placement opening is provided on the side wall of the support plate, which is connected to the through hole. A sampling tube is provided at the lower end of the support plate, and the sampling tube is positioned corresponding to the through hole. Although the device can achieve simple single sampling, it has the following problems: First, the sampling process relies on manual pressing, which is not only laborious but also inefficient and inconvenient for large-scale sampling; Second, after a single sampling, the sampling mechanism needs to be manually sterilized and disinfected repeatedly, which is a frequent process and not suitable for sampling in research on grape leaf curl disease. Summary of the Invention

[0004] Based on the aforementioned background technology needs, this application provides a grape leaf curl disease leaf sampling device, which is suitable for sampling grape leaves in research on grape leaf curl disease.

[0005] To achieve the above objectives, the technical solution of this application is as follows:

[0006] A sampling device for grape leaf curl disease includes a sampling component and a disinfection component. The sampling component includes a fixing plate, a pressure plate, a sampling punch, a punch driving mechanism, and a sampling tube. One end of the fixing plate has a positioning hole extending along a first direction. The punch driving mechanism is disposed on the fixing plate and drives the sampling punch. The punch driving mechanism drives the sampling punch to move within the positioning hole along the first direction. The pressure plate is disposed on the side of the fixing plate opposite to the punch driving mechanism and maintains a leaf clamping gap between the pressure plate and the fixing plate. One end of the pressure plate has a sampling hole extending along the first direction. The sampling tube is detachably disposed within the sampling hole, and its opening faces the opposite direction to the first direction. The disinfection component includes a disinfectant cavity disposed on the fixing plate and a spraying mechanism. The disinfectant cavity is connected to the inlet of the spraying mechanism, and the outlet of the spraying mechanism extends to the vicinity of the sampling punch and is used to spray disinfectant toward the sampling punch.

[0007] Preferably, a rotary reset mechanism is provided on the side of the fixed plate away from the punch drive mechanism. The pressure plate is driven by the rotary reset mechanism. The rotary reset mechanism is used to rotate the pressure plate in the same plane so that the sampling hole is opposite to or misaligned with the positioning hole.

[0008] Preferably, the sampling assembly further includes a protective housing that covers one side surface of the fixing plate and contains the punch drive mechanism and the disinfection assembly within its space.

[0009] Preferably, the punch driving mechanism includes a pneumatic telescopic cylinder, an air source, and a first actuation switch. The air source is connected to the pneumatic telescopic cylinder, and the first actuation switch is electrically connected to the air source. The sampling punch is detachably mounted on one end of the telescopic rod of the pneumatic telescopic cylinder.

[0010] Preferably, the sampling punch includes a slicing rod, a return spring, and a push rod. One end of the slicing rod is connected to the telescopic rod of the pneumatic telescopic cylinder, and the other end has an axial mounting hole. The push rod is slidably disposed in the mounting hole along the axial direction of the slicing rod. The return spring is disposed between the mounting hole and the push rod, and its two ends are respectively connected to the mounting hole and the push rod. The return spring is used to make one end of the push rod protrude out of the mounting hole when no force is applied.

[0011] Preferably, the protective shell has a liquid filling port on its surface, one side of which is connected to the disinfectant cavity, and a plug is detachably provided at the liquid filling port.

[0012] Preferably, the spraying mechanism includes a liquid pump, a nozzle, and a sensor switch. The nozzle is located on the fixed plate adjacent to the moving path of the sampling punch, and its nozzle faces the end of the sampling punch away from the punch drive mechanism. The inlet of the liquid pump is connected to the disinfectant chamber, and the outlet of the liquid pump is connected to the nozzle. The sensor switch is electrically connected to the liquid pump and is used to start or stop the liquid pump from supplying liquid based on the position information of the sampling punch.

[0013] Preferably, the grape leaf curl disease sampling device further includes an extension component, which includes a telescopic adjustment rod and a handle disposed at one end of the telescopic adjustment rod, and the other end of the telescopic adjustment rod is connected to at least a portion of the sampling component.

[0014] Preferably, the extension assembly further includes an angle adjustment motor and a second action switch. The end of the telescopic adjustment rod away from the handle is hinged to a hinge seat that can rotate up and down via a hinge shaft. The hinge seat is fixedly connected to at least part of the sampling assembly. The angle adjustment motor is fixed to the hinge seat and its drive shaft is drivenly connected to the hinge shaft. The second action switch is electrically connected to the angle adjustment motor.

[0015] Preferably, the second actuation switch is mounted on the surface of the handle.

[0016] By adopting the above technical solution, compared with the prior art, this application has at least the following beneficial effects:

[0017] After the operator takes a sample using the sampling component, the sampling nozzle is promptly disinfected using the disinfection component. This prevents the sampling nozzle from contaminating the sample or becoming a source of virus transmission during contact with the leaf. Replacing the sampling tube after disinfection allows for continuous sampling, improving the efficiency and safety of sample collection for grape leaf curl disease. It simplifies the sampling process, saves time and effort, and eliminates the need for human contact with the leaf. Attached Figure Description

[0018] Figure 1 This is a front view of the grape leaf curl disease sampling device in the embodiment.

[0019] Figure 2 This is a schematic diagram of the internal structure of the grape leaf curl disease sampling device from any perspective in the embodiment.

[0020] Figure 3 This is a partial structural schematic diagram of the grape leaf curl disease sampling device from another perspective in the embodiment.

[0021] Figure 4 This is a partial sectional view A of the sampling component in the embodiment (taken from...). Figure 2 ).

[0022] Figure 5 This is a partially enlarged cross-sectional view B of the sampling component in the embodiment.

[0023] Figure 6 This is a partial cross-sectional view (CC) of the sampling punch in the embodiment.

[0024] In the figure: Sampling component 10, fixing plate 11, positioning hole 111, pressure plate 12, sampling hole 121, sampling punch 13, slicing rod 131, mounting hole 1311, limit block 1312, return spring 132, top rod 133, punch drive mechanism 14, pneumatic telescopic cylinder 141, air source 142, first action switch 143, sampling tube 15, rotational reset mechanism 16, protective shell 17, plug 171, disinfection component 20, disinfectant cavity 21, spraying mechanism 22, liquid pump 221, nozzle 222, inductive switch 223, sampling punch position sensor 2231, extension component 30, telescopic adjustment rod 31, hinge shaft 311, hinge seat 312, handle 32, angle adjustment motor 33, second action switch 331.

[0025] It should be noted that, in order to demonstrate more details of this application and to help people better understand its contents, the above appendix... Figure 2 Appendix Figure 3 Partial partitions were made separately, as shown in the attached document. Figure 2 The telescopic adjustment rod 31 part is omitted in the text. Figure 3 The structure of the upper part of the protective casing has been omitted in the accompanying drawings to reduce the size of the drawings and showcase other key details. These omissions will not negatively impact the product details presented in this application. Detailed Implementation

[0026] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The technical solutions of this application will be further described below with reference to the accompanying drawings of the embodiments, and this application is not limited to the following specific implementation methods.

[0027] It should be understood that the same or similar reference numerals in the accompanying drawings of the embodiments correspond to the same or similar components. In the description of this application, it should be understood that if terms such as "upper," "lower," "inner," "outer," "left," "right," "front," "rear," "top," and "bottom" indicate directions or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, they are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the structure or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms describing positional relationships in the accompanying drawings are for illustrative purposes only and should not be construed as limitations on this patent. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0028] The following is in conjunction with the appendix Figure 1 To be continued Figure 6 The present application will be further described in detail with reference to specific embodiments.

[0029] This application discloses a sampling device for grape leaf curl disease (hereinafter referred to as "sampling device"), including a sampling component 10 for cutting and sampling leaves and a disinfection component 20 for disinfection after sampling to facilitate continuous sampling. The sampling component 10 includes a fixing plate 11, a pressure plate 12, a sampling punch 13, a punch driving mechanism 14, and a sampling tube 15. One end of the fixing plate 11 has a positioning hole 111 extending through it along a first direction, which is perpendicular to the upper plane of the fixing plate 11 and vertically upwards. The direction of the lower plane (i.e., downward in all figures) is where the punch drive mechanism 14 is located above the fixed plate 11 and is connected in a transmission engagement with the sampling punch 13. The punch drive mechanism 14 includes any drive mechanism or motion mechanism capable of driving the sampling punch 13 to move downward through the positioning hole 111 along the aforementioned first direction. The pressure plate 12 is located on the side of the fixed plate 11 away from the aforementioned punch drive mechanism 14 (i.e., the lower surface of the fixed plate 11), and the upper surface of the pressure plate 12 is aligned with the lower surface of the fixed plate 11. There is a blade clamping gap, which allows the curled blade to enter the gap between the pressure plate 12 and the fixed plate 11 in parallel. One end of the pressure plate 12 is provided with a sampling hole 121 through it along the first direction. The sampling tube 15 is detachably installed in the sampling hole 121 and its opening faces the opposite direction to the first direction (upward in the attached drawings of this application). Specifically, the sampling tube 15 applicable to this application is similar in structure to the commonly used ordinary test tubes on the market, and based on the structure of the test tube, its opening is provided with a sampling port on the outside. The flanges fit together or connect to allow the sampling tube 15 to be detachably fixed below the positioning hole 111; the above-mentioned disinfection assembly 20 includes a disinfectant cavity 21 disposed above the fixing plate 11 and any spraying mechanism 22 that can spray liquid disinfectant in a pressurized manner. The inlet of the spraying mechanism 22 is connected to the disinfectant cavity 21, and its outlet (i.e., the spray nozzle) extends to the vicinity of the sampling punch 13 and can spray the disinfectant solution remaining in the disinfectant cavity 21 toward the sampling punch 13.

[0030] Based on the above embodiments, one sampling process using the sampling device of this application is as follows: First, a clean sampling tube 15 with its opening facing upwards is clamped into the sampling port; second, the sampling assembly 10 is moved close to the blade to be sampled, so that the blade to be sampled enters the blade clamping gap parallel to it, and then the punch drive mechanism 14 is operated to drive the sampling punch 13 to move downwards, using the lower end of the sampling punch 13 to squeeze the blade and punch the fragments cut off from the blade into the sampling tube 15; after completing one sampling, the sampling tube 15 is removed and the spray mechanism 22 is operated to spray disinfectant onto the reset sampling punch 13, so that the sprayed disinfectant mist disinfects the sampling punch 13. Specifically, the disinfectant can be 75% alcohol atomized liquid, with 0.1 to 0.2 ml sprayed at a time.

[0031] Using the above-described sampling device has at least the following beneficial effects:

[0032] After the operator takes a sample using the sampling component 10, the sampling punch 13 is disinfected in a timely manner using the disinfection component 20. This prevents the sampling punch 13 from contaminating the sample or becoming a source of virus transmission during contact with the leaf. After disinfection, the sampling tube 15 is replaced, which allows for continuous sampling. This improves the efficiency and safety of sample collection for grape leaf curl disease, simplifies the sampling process, and saves time and effort. The entire sampling process does not require human contact with the leaf.

[0033] In addition, this application provides some more specific embodiments to improve the above-mentioned grape leaf curl disease leaf sampling device.

[0034] Based on the structure of the above embodiment, in order to improve the replacement efficiency and safety of the sampling tube 15, a rotary reset mechanism 16 is provided on the side of the fixed plate 11 away from the punch drive mechanism 14 (i.e., the lower surface). The pressure plate 12 is in transmission cooperation with the rotary reset mechanism 16. Specifically, the rotary reset mechanism 16 includes a positioning pin provided on the lower side of the fixed plate 11. A reset torsion spring is sleeved on the lower end of the positioning pin, and the pressure plate 12 is slidably sleeved on the upper end of the positioning pin. The two ends of the reset torsion spring are fixed to the positioning pin and the pressure plate 12, respectively.

[0035] When using this application, the pressure plate 12 can rotate around the positioning pin in a plane parallel to the fixed plate 11. When the sampling hole 121 is misaligned with the positioning hole 111, the sampling tube 15 can be disassembled or installed, or the spraying mechanism 22 can be operated to disinfect the sampling punch 13. In this embodiment, the sampling hole 121 is a countersunk hole with an upper diameter larger than the lower diameter, and the upper diameter of the countersunk hole is larger than the flange diameter of the sampling tube 15, larger than the lower diameter of the countersunk hole, and larger than the tube body diameter of the sampling tube 15. The operator can place the sampling tube 15 into the countersunk hole. Replacement can be completed by inserting the sampling tube 15 into the sampling hole 121. During disassembly, the sampling tube 15 can be removed, which can avoid excessive disassembly and sample ejection. Furthermore, by rotating the reset mechanism 16 to offset the sampling hole 121 and the positioning hole 111, it can also prevent disinfectant droplets from falling into the sampling tube 15 during disinfection and affecting the sample quality. After the sampling tube 15 is replaced, the pressure plate 12 can automatically reset under the rebound action of the reset torsion spring and be positioned so that the sampling tube 15 and the sampling punch 13 are on the same axis, so as to perform repeated sampling.

[0036] Furthermore, in order to form a disinfectant atomization space, the sampling component 10 also includes a protective housing 17. The protective housing 17 is covered and fixed to the upper side of the fixing plate 11 by means of fasteners, screws, screw holes, etc., and the punch drive mechanism 14 and the disinfection mechanism are both included in its (referring to the protective housing 17) space.

[0037] Specifically, the protective housing 17 is divided into upper and lower hollow structures by a support plate located in the middle of its internal space. The punch drive mechanism 14 is located on the support plate, and the disinfection component 20 is located in the space below the support plate. The space below the support plate can prevent the disinfectant from spreading too widely and affecting the sampling blade. The protective housing 17 protects key components such as the sampling punch 13, the punch drive mechanism 14, and the spray mechanism 22, preventing damage caused by bumps during sampling and extending the service life of this application. In order to facilitate the replenishment of disinfectant into the disinfectant cavity 21, one side of the disinfectant cavity 21 is integrated with the side wall of the protective housing 17. A liquid filling port is provided on the side of the protective housing 17 adjacent to the disinfectant cavity 21, and a plug 171 is detachably provided at the liquid filling port. The operator can remove or install the plug 171 to replenish disinfectant or seal the liquid filling port.

[0038] In one embodiment, to ensure that the sampling punch 13 has sufficient power to penetrate the blade and perform sampling, the punch drive mechanism 14 includes a pneumatic telescopic cylinder 141, an air source 142, and a first actuation switch 143. The pneumatic telescopic cylinder 141 is preferably a miniature cylinder, such as the miniature cylinder disclosed in the utility model with publication number CN217271152U. The air source 142 is preferably a miniature vacuum pump, such as the adjustable speed miniature vacuum pump C30L-42X from Hilintec. The air inlet and outlet of the pneumatic telescopic cylinder 141 are connected to the power output port and return port of the air source 142. The first actuation switch 143 electrically connects the power supply to the air source 142 to form a control circuit. One end of the telescopic rod of the pneumatic telescopic cylinder 141 is detachably connected to the sampling punch 13 via a thread.

[0039] When using the above-mentioned detection device, the operator triggers the switch to energize the air source 142 and supply or extract air to the pneumatic telescopic cylinder 141, so as to drive the sampling punch 13 to move downward quickly to impact and sample the blade.

[0040] Furthermore, to prevent the sampled blade from sticking to the lower end of the sampling punch 13 due to wear, thus preventing the sample from successfully entering the sampling tube 15, the sampling punch 13 includes a slicing rod 131, a return spring 132, and a push rod 133. The upper end of the slicing rod 131 is connected to the telescopic rod of the pneumatic telescopic cylinder 141, and the lower end is provided with an mounting hole 1311 along the axial direction. Specifically, the lower end of the mounting hole 1311 and the side wall of the slicing rod 131 are chamfered to form a cutting edge, which can cut out a circular blade sample during the contact with the blade; the upper bottom of the mounting hole 1311 extends downward to provide a limiting block 1312, the push rod 133 can slide up and down in the mounting hole 1311, the return spring 132 is located between the mounting hole 1311 and the slide rod, and the upper end of the return spring 132 is fixedly sleeved on the limiting block 1312, and the lower end is fixed to the push rod 133. The return spring 132 can make the lower end of the push rod 133 protrude out of the mounting hole 1311 in the natural state (i.e., the unforced state). Specifically, as the sampling punch 13 penetrates the blade downwards, the cutting edge at the lower end of the slicing rod 131 cuts the blade. When the lower end of the slicing rod 131 has completely penetrated the blade, the push rod 133 extends out of the mounting hole 1311 due to the lack of upward support force and the rebound action of the return spring 132, causing the cut blade sample to separate from the slicing rod 131 and fall into the sampling tube 15 for collection.

[0041] In a preferred embodiment, the above-mentioned spraying mechanism 22 includes a liquid pump 221, a nozzle 222, and an inductive switch 223. The nozzle 222 is located on the upper surface of the fixed plate 11 adjacent to the moving path of the sampling punch 13, and its (referring to the nozzle 222) nozzle faces the end (i.e. the lower end) of the sampling punch 13 away from the punch drive mechanism 14. The liquid pump 221 is a miniature electric liquid pump 221, whose inlet is connected to the disinfectant cavity 21 through a pipeline, and whose outlet is connected to the inlet of the nozzle 222. The inductive switch 223 is electrically connected to the liquid pump 221.

[0042] Specifically, to enable the nozzle 222 to accurately disinfect the reset sampling punch 13, the aforementioned inductive switch 223 is preferably a photoelectric position sensing switch 223, including a signal receiving control module and a sampling punch position sensor 2231. The receiving end of the signal receiving control module is opposite to the sampling punch position sensor 2231 and is spaced apart on both sides of the moving path of the sampling punch 13. The sampling punch position sensor 2231 continuously emits light signals to the signal receiving control module. When the sampling punch 13 blocks the light signal during its movement, preventing the signal receiving control module from sensing the light signal, the circuit is not broken, i.e., the liquid pump 221 is on standby. When the sampling punch 13 resets after sampling, the signal receiving control module senses the light signal (i.e., the position information of the sampling punch 13) and energizes the circuit. The liquid pump 221 draws out the disinfectant and sprays it through the nozzle 222 to the lower end of the sampling punch 13 for disinfection.

[0043] Furthermore, to increase the sampling range of the sampling component 10 of this application, the above-mentioned sampling device also includes an extension component 30. Based on the structure of the above embodiment, the extension component 30 includes a telescopic adjustment rod 31, which is a telescopic sleeve structure. One end of the telescopic adjustment rod 31 is provided with a handle 32, and the other end is connected to at least a portion of the sampling component 10. In a preferred embodiment, the front end of the telescopic adjustment rod 31 is connected to the protective housing 17. By extending the telescopic adjustment rod 31, the operator can hold the handle 32 and extend the sampling component 10 to a position that is difficult for the human hand to reach, so as to sample grape leaves at higher or deeper positions. Specifically, the first action switch 143 can also be provided on the handle 32, so that the operator can easily trigger the first action switch 143 to control the sampling timing.

[0044] To improve portability, the extension assembly 30 further includes an angle adjustment motor 33 and a second actuation switch 331. The end of the telescopic adjustment rod 31 furthest from the handle 32 is hinged to a hinge seat 312 via a hinge shaft 311. One side of the hinge seat 312 is fixedly connected to at least a portion of the sampling assembly 10, such as the protective housing 17 or the fixing plate 11. The fixed end of the angle adjustment motor 33 is fixed to one side of the hinge seat 312, and its drive shaft is coaxially connected to the hinge shaft 311. The second actuation switch 331 is mounted on the handle 32 and electrically connected to the angle adjustment motor 33. The second actuation switch 331 drives the angle adjustment motor 33 to rotate the hinge seat 312 up and down to a suitable angle, allowing the leaf clamping gap of the sampling assembly 10 (the gap between the fixing plate 11 and the pressure plate 12 mentioned above) to accommodate grape leaves growing in different directions, thereby improving sampling portability and sampling success rate.

[0045] By combining the various structures and features in the above embodiments, the sampling and adjustment functions of the above-mentioned grape leaf curl disease sampling device have been improved to enhance sampling efficiency, sampling safety, and human-machine ergonomics.

[0046] Obviously, the above embodiments of this application are merely examples for clearly illustrating this application, and are not intended to limit the implementation of this application. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A grapevine leafroll leaf sampling device, characterized by, include A sampling assembly includes a fixed plate, a pressure plate, a sampling punch, a punch drive mechanism, and a sampling tube. One end of the fixed plate has a positioning hole extending along a first direction. The punch drive mechanism is mounted on the fixed plate and drives the sampling punch, driving it to move within the positioning hole along the first direction. The pressure plate is located on the side of the fixed plate opposite to the punch drive mechanism and maintains a blade clamping gap with the fixed plate. One end of the pressure plate has a sampling hole extending along the first direction. The sampling tube is detachably mounted within the sampling hole, with its opening facing opposite to the first direction. The disinfection assembly includes a disinfectant cavity disposed on the fixed plate and a spraying mechanism. The disinfectant cavity is connected to the inlet of the spraying mechanism, and the outlet of the spraying mechanism extends to the vicinity of the sampling punch and is used to spray disinfectant toward the sampling punch.

2. The grapevine leafroll-affected leaf sampling device of claim 1, wherein, A rotary reset mechanism is provided on the side of the fixed plate away from the punch drive mechanism. The pressure plate is driven by the rotary reset mechanism. The rotary reset mechanism is used to rotate the pressure plate in the same plane so that the sampling hole is opposite to or misaligned with the positioning hole.

3. The grapevine leaf sampling device of claim 1, wherein, The sampling assembly also includes a protective housing that covers one side surface of the fixing plate and contains the punch drive mechanism and the disinfection assembly within its space.

4. The grapevine leafroll-affected leaf sampling device of claim 3, wherein, The punch drive mechanism includes a pneumatic telescopic cylinder, an air source, and a first actuation switch. The air source is connected to the pneumatic telescopic cylinder, and the first actuation switch is electrically connected to the air source. The sampling punch is detachably mounted on one end of the telescopic rod of the pneumatic telescopic cylinder.

5. The grapevine leaf sampling device of claim 4, wherein, The sampling punch includes a slicing rod, a return spring, and a push rod. One end of the slicing rod is connected to the telescopic rod of the pneumatic telescopic cylinder, and the other end has an axial mounting hole. The push rod is slidably disposed in the mounting hole along the axial direction of the slicing rod. The return spring is disposed between the mounting hole and the push rod, and its two ends are respectively connected to the mounting hole and the push rod. The return spring is used to make one end of the push rod protrude out of the mounting hole when no force is applied.

6. The grapevine leaf sampling device of claim 3, wherein, The protective shell has a liquid filling port on its surface. One side of the liquid filling port is connected to the disinfectant cavity. A plug is detachably installed at the liquid filling port.

7. The grapevine leaf sampling device of claim 1, wherein, The spraying mechanism includes a liquid pump, a nozzle, and a sensor switch. The nozzle is located on the fixed plate adjacent to the moving path of the sampling punch, and its nozzle faces the end of the sampling punch away from the punch drive mechanism. The inlet of the liquid pump is connected to the disinfectant cavity, and the outlet of the liquid pump is connected to the nozzle. The sensor switch is electrically connected to the liquid pump and is used to start or stop the liquid pump from supplying liquid based on the position information of the sampling punch.

8. The grapevine leaf sampling device according to any one of claims 1 to 7, wherein, It also includes an extension assembly, which includes a telescopic adjustment rod and a handle disposed at one end of the telescopic adjustment rod, the other end of which is connected to at least a portion of the sampling assembly.

9. The grapevine leaf sampling device of claim 8, wherein, The extension assembly further comprises an angle adjusting motor and a second action switch, one end of the telescopic adjusting rod away from the handle is hingedly connected with a hinged seat through a hinged shaft, the hinged seat is fixedly connected with at least part of the sampling assembly, the angle adjusting motor is fixed to the hinged seat and a driving shaft thereof is in driving connection with the hinged shaft, and the second action switch is in electrical connection with the angle adjusting motor.

10. The grapevine leaf sampling device of claim 9, wherein, The second action switch is mounted on the surface of the handle.

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