An integrated gene extraction and amplification detection device

Abstract

The utility model discloses an integrated gene extraction and amplification detection equipment relates to gene detection device technical field, this integrated gene extraction and amplification detection equipment includes detection equipment main part. This integrated gene extraction and amplification detection equipment combines gene extraction with amplification appearance, improves gene detection efficiency, and through setting up the cleaning component in the anti -dripping board inside, and setting up the waste liquid collection frame at the bottom of anti -dripping board, under the cooperation of micro motor, it is convenient to tilt anti -dripping board, when anti -dripping board is inclined to the top drop sample and is washed, further can open waste liquid outlet automatically, make the waste liquid of washing can under the gravity effect automatic flow into the inside of waste liquid collection frame and collect, simultaneously, after washing, through the reset of anti -dripping board, make sealing plate sealed waste liquid outlet automatically, avoid the waste liquid in the waste liquid collection frame with airflow, contact diffusion to other areas, further can improve the accuracy of multiple group gene detection.

Description

Technical Field

[0001] This utility model relates to the field of gene detection device technology, specifically an integrated gene extraction and amplification detection device. Background Technology

[0002] With the development of molecular biology technology, integrated gene extraction and amplification detection equipment has been widely used in clinical diagnosis, disease screening and scientific research experiments due to its advantages such as convenient operation and rapid detection. However, in actual application, the equipment's anti-pollution performance and waste liquid treatment mechanism are still insufficient.

[0003] Currently, most integrated gene extraction and amplification detection devices use the magnetic rod method for sample processing during nucleic acid extraction. When the magnetic rod is transferred between different reagent tubes, liquid residue easily remains on its surface. Although some devices are equipped with anti-drip plates to reduce liquid dripping, sample waste liquid containing impurities, unbound nucleic acids, or pathogens remains on the top of the anti-drip plate during the drip-proofing process. Due to the lack of effective cleaning measures, these residual liquids can easily contaminate subsequent samples or reagents during device operation and subsequent operations, leading to false positives or false negatives in the test results, which seriously affects the accuracy and reliability of gene detection. To address the shortcomings of existing technologies, we propose an integrated gene extraction and amplification detection device to solve the above problems. Utility Model Content

[0004] To achieve the above objectives, this utility model is implemented through the following technical solution: an integrated gene extraction and amplification detection device, comprising a detection device body, a magnetic rod module disposed inside the detection device body, the magnetic rod module being moved inside the detection device body by a robotic arm, a reagent tube disposed at the bottom of the magnetic rod module, an amplification instrument disposed on one side of the reagent tube, an anti-drip plate disposed on one side of the magnetic rod module, the anti-drip plate being moved inside the detection device body by a driving component, an installation frame being fixedly sleeved on the outer peripheral wall of the anti-drip plate, and a cleaning component disposed on one side of the inner side of the installation frame;

[0005] A waste liquid collection frame is located directly below the anti-drip plate. A connecting shaft is fixedly installed on one outer wall of the mounting frame. The mounting frame tilts the anti-drip plate around the connecting shaft. A waste liquid outlet is opened on the side of the anti-drip plate away from the connecting shaft. A sealing plate is attached to the top of the waste liquid outlet to seal it. The sealing plate is installed with the waste liquid collection frame via a mounting assembly. Tilting the anti-drip plate opens the waste liquid outlet.

[0006] Preferably, the drive assembly includes a mounting plate, a drive cylinder, a mounting side plate, a support base plate, and a limiting side plate. The mounting plate is slidably connected to the top of the main body of the detection equipment, and the drive cylinder is fixedly installed at the bottom of the mounting plate.

[0007] Preferably, the output end of the drive cylinder is fixedly installed with the mounting side plate, the support base plate is fixedly installed on the bottom side of the mounting side plate, the limiting side plate is fixedly installed on the top side of the support base plate, and the waste liquid collection frame is slidably connected between the support base plate and the anti-drip plate.

[0008] Preferably, a micro motor is fixedly installed on one outer wall of the mounting side plate, the output end of the micro motor is fixedly connected to one end of the connecting shaft, and the two ends of the connecting shaft are rotatably connected to the mounting side plate.

[0009] Preferably, the cleaning assembly includes a disinfectant tank, a high-pressure nozzle pipe, and a cleaning scraper. The disinfectant tank is fixedly installed on the outer wall of the mounting side plate, and the high-pressure nozzle pipe is fixedly installed at one end inside the mounting frame.

[0010] Preferably, the high-pressure nozzle is connected to the disinfectant tank via a connecting hose, the cleaning scraper is slidably connected inside the mounting frame, and the bottom of the cleaning scraper is in contact with the top of the anti-drip plate.

[0011] Preferably, the installation assembly includes a support rod, a threaded component, a rubber pad threaded cylinder, and a threaded ring. The top end of the support rod passes through the waste liquid outlet and is fixedly installed on both sides of the bottom of the sealing plate. The threaded cylinder is located on both sides of the bottom of the waste liquid collection frame.

[0012] Preferably, the threaded component is fixedly installed at the bottom end of the support rod, the bottom end of the threaded component passes through the waste liquid collection frame and is threadedly connected to the threaded cylinder, the threaded ring is fixedly installed at the top of the threaded cylinder, the threaded ring is threadedly connected to the bottom of the waste liquid collection frame, the rubber pad is fixedly sleeved on the outer peripheral wall of the support rod, and the rubber pad is attached to the top of the waste liquid collection frame.

[0013] This utility model discloses an integrated gene extraction and amplification detection device, which has the following beneficial effects: This integrated gene extraction and amplification detection device, by setting a cleaning component inside the anti-drip plate and a waste liquid collection frame at the bottom of the anti-drip plate, facilitates tilting of the anti-drip plate with the assistance of a micro-motor. During the cleaning process of the anti-drip plate cleaning the sample dripping from the top, the waste liquid outlet is automatically opened, allowing the cleaning waste liquid to automatically flow into the waste liquid collection frame for collection under gravity. Simultaneously, after cleaning, the anti-drip plate resets, automatically sealing the waste liquid outlet through a sealing plate. This prevents the waste liquid inside the bottom waste liquid collection frame from spreading to other areas with airflow and contact, avoiding contamination of subsequent samples or reagents, and preventing residual liquid on the top of the anti-drip plate from interfering with subsequent operations, thereby improving the accuracy of gene detection. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the main body of the testing equipment of this utility model;

[0017] Figure 3 This is a schematic diagram of the connection structure between the drive assembly and the anti-drip plate of this utility model;

[0018] Figure 4 This is a schematic diagram of the anti-drip plate structure of this utility model after tilting;

[0019] Figure 5 This is a schematic diagram showing the installation positions of the mounting frame and the waste liquid collection frame of this utility model;

[0020] Figure 6 This is an exploded view of the anti-drip plate and waste liquid collection frame of this utility model;

[0021] Figure 7 This is a side cross-sectional view of the waste liquid collection frame of this utility model.

[0022] In the diagram: 1. Main body of the testing equipment; 2. Magnetic rod module; 21. Robotic arm; 3. Reagent tube; 4. Amplifier; 5. Anti-drip plate; 51. Waste liquid outlet; 6. Drive assembly; 61. Mounting plate; 62. Drive cylinder; 63. Mounting side plate; 64. Support base plate; 65. Limiting side plate; 7. Mounting frame; 8. Cleaning assembly; 81. Disinfectant tank; 82. High-pressure nozzle pipe; 83. Cleaning scraper; 9. Waste liquid collection frame; 91. Sealing plate; 10. Connecting shaft; 101. Micro motor; 11. Mounting assembly; 111. Support rod; 112. Threaded part; 113. Rubber pad; 114. Threaded cylinder; 115. Threaded ring. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments of this utility model are described clearly and completely. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0024] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0025] This utility model discloses an integrated gene extraction and amplification detection device.

[0026] According to the appendix Figure 1-7 As shown, the device includes a main body 1 for the detection equipment. Inside the main body 1 is a magnetic rod module 2, which moves within the main body 1 via a robotic arm 21. A reagent tube 3 is located at the bottom of the magnetic rod module 2. The reagent tube 3 contains pre-filled or sequentially added reagents such as lysis buffer, binding buffer, washing buffer, and elution buffer, as well as the biological sample to be extracted. An amplification instrument 4 is located on one side of the reagent tube 3, used for gene amplification. An anti-drip plate 5 is located on one side of the magnetic rod module 2, which moves into the main body 1 via a drive assembly 6. A mounting frame 7 is fixedly fitted to the outer periphery of the anti-drip plate 5, and a cleaning assembly 8 is located on one side of the mounting frame 7. During use, multiple reagent tubes 3 containing nucleic acid genes are first placed inside the main body 1. With the assistance of the robotic arm 21, the magnetic rod module 2 moves to the reagent tube 3 to extract the genes. The magnetic rod module 2 is the core component directly involved in nucleic acid separation in the gene extraction device, primarily functioning through magnetic adsorption. This is the existing working principle of gene extraction and will not be elaborated further.

[0027] A waste liquid collection frame 9 is located directly below the anti-drip plate 5. A connecting shaft 10 is fixedly installed on one outer wall of the mounting frame 7. The mounting frame 7 tilts the anti-drip plate 5 around the connecting shaft 10. A waste liquid outlet 51 is opened on the side of the anti-drip plate 5 away from the connecting shaft 10. A sealing plate 91 is attached to the top of the waste liquid outlet 51, sealing the waste liquid outlet 51. The sealing plate 91 is installed with the waste liquid collection frame 9 via the mounting assembly 11. The tilting of the anti-drip plate 5 opens the waste liquid outlet 51, allowing the magnetic rod module 2 to... During the extraction and transfer process, with the cooperation of the drive component 6, the anti-drip plate 5 can shield the bottom of the magnetic rod module 2, thus avoiding the risks of contamination, reagent waste, and result errors caused by liquid residue or dripping. After the magnetic rod module 2 completes the operation of the previous reagent tube 3, liquid may remain on its surface. If it is directly transferred to the next reagent tube 3, the dripping droplets may carry residual impurities or non-target nucleic acids, leading to cross-contamination between different samples or reagents. The anti-drip plate 5 can avoid cross-contamination.

[0028] The drive assembly 6 includes a mounting plate 61, a drive cylinder 62, a mounting side plate 63, a support base plate 64, and a limiting side plate 65. The mounting plate 61 is slidably connected to the top of the main body 1 of the detection equipment. The drive cylinder 62 is fixedly installed at the bottom of the mounting plate 61. The output end of the drive cylinder 62 is fixedly installed with the mounting side plate 63. The support base plate 64 is fixedly installed on one side of the bottom of the mounting side plate 63. The limiting side plate 65 is fixedly installed on one side of the top of the support base plate 64. The waste liquid collection frame 9 is slidably connected between the support base plate 64 and the anti-drip plate 5.

[0029] A micro motor 101 is fixedly installed on one outer wall of the mounting side plate 63. The output end of the micro motor 101 is fixedly connected to one end of the connecting shaft 10, and both ends of the connecting shaft 10 are rotatably connected to the mounting side plate 63. The cleaning assembly 8 includes a disinfectant tank 81, a high-pressure nozzle pipe 82, and a cleaning scraper 83. The disinfectant tank 81 is fixedly installed on the outer wall of the mounting side plate 63, and the high-pressure nozzle pipe 82 is fixedly installed inside one end of the mounting frame 7. By starting the micro motor 101, the mounting frame 7 rotates around the connecting shaft 10, thereby tilting the anti-drip plate 5. The high-pressure nozzle pipe 82 operates, spraying disinfectant onto the surface of the anti-drip plate 5. With the cooperation of the cleaning scraper 83, the top of the anti-drip plate 5 is cleaned. At the same time, the tilting of the anti-drip plate 5 facilitates the discharge of the sample and disinfectant together through the waste liquid outlet 51 into the waste liquid collection frame 9 for collection. For details, please refer to the appendix. Figure 5 This facilitates the cleaning of liquid on the top of the anti-drip plate 5, ensuring clarity and uniformity. The micro motor 101 drives the anti-drip plate 5 to return to its original position, automatically sealing the waste liquid outlet 51 with the sealing plate 91. This prevents the anti-drip plate 5 from contacting the magnetic rod module 2 during the next gene extraction process, thus avoiding the liquid from spreading to other areas with airflow and contaminating subsequent samples or reagents.

[0030] The high-pressure nozzle 82 is connected to the disinfectant tank 81 via a connecting hose. The cleaning scraper 83 is slidably connected inside the mounting frame 7, with the bottom of the cleaning scraper 83 fitting against the top of the anti-drip plate 5. The mounting assembly 11 includes a support rod 111, a threaded component 112, a rubber pad 113, a threaded cylinder 114, and a threaded ring 115. The top of the support rod 111 passes through the waste liquid outlet 51 and is fixedly installed on both sides of the bottom of the sealing plate 91. The threaded cylinder 114 is located on both sides of the bottom of the waste liquid collection frame 9. When the anti-drip plate 5 is tilted to clean the sample dripping from the top, the support rod... The positions of 111 and sealing plate 91 remain unchanged, thereby automatically opening the waste liquid outlet 51. This allows the cleaning waste liquid to automatically flow into the waste liquid collection frame 9 for collection under gravity. Simultaneously, after cleaning, the anti-drip plate 5 resets, automatically sealing the waste liquid outlet 51 through sealing plate 91. This prevents the waste liquid inside the bottom waste liquid collection frame 9 from spreading to other areas with airflow and contaminating subsequent samples or reagents, avoiding residual liquid interference with subsequent operations, and thus improving the accuracy of multi-gene detection.

[0031] A threaded component 112 is fixedly installed at the bottom end of the support rod 111. The bottom end of the threaded component 112 passes through the waste liquid collection frame 9 and is threadedly connected to the threaded cylinder 114. Through the threaded connection between the threaded cylinder 114, the threaded component 112, and the waste liquid collection frame 9, the sealing plate 91 can be connected and fixed to the waste liquid collection frame 9, and the sealing plate 91 is limited and installed on the top of the anti-drip plate 5. The sealing plate 91 seals the waste liquid outlet 51. A threaded ring 115 is fixedly installed on the top of the threaded cylinder 114 and threadedly connected to the bottom of the waste liquid collection frame 9. A rubber pad 113 is fixedly sleeved on the outer peripheral wall of the support rod 111 and fits against the outer peripheral wall of the support rod 111. After the overall gene extraction is completed, the waste liquid collection frame 9 can be removed separately from the top of the support base plate 64 to clean the waste liquid collected inside the waste liquid collection frame 9. When disassembling the waste liquid collection frame 9, first rotate the threaded cylinder 114 to disengage the threaded cylinder 114 from the bottom of the waste liquid collection frame 9 by the threaded part 112. Then lift the sealing plate 91 upwards, so that the sealing plate 91 can drive the two sets of support rods 111 and threaded parts 112 to disengage from the top of the anti-drip plate 5, and the waste liquid collection frame 9 is no longer restricted. Then the waste liquid collection frame 9 can be directly removed from the top of the support base plate 64.

[0032] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. An integrated gene extraction and amplification detection device, comprising a detection device body (1), wherein a magnetic rod module (2) is disposed inside the detection device body (1), the magnetic rod module (2) is moved inside the detection device body (1) by a robotic arm (21), a reagent tube (3) is disposed at the bottom of the magnetic rod module (2), and an amplification instrument (4) is disposed on one side of the reagent tube (3), characterized in that: A drip-proof plate (5) is provided on one side of the magnetic rod module (2). The drip-proof plate (5) is moved into the main body (1) of the detection device by the drive component (6). A mounting frame (7) is fixedly sleeved on the outer peripheral wall of the drip-proof plate (5). A cleaning component (8) is provided on one side of the inner side of the mounting frame (7). A waste liquid collection frame (9) is provided directly below the anti-drip plate (5). A connecting shaft (10) is fixedly installed on one side of the outer wall of the mounting frame (7). The mounting frame (7) tilts the anti-drip plate (5) with the connecting shaft (10) as the axis. A waste liquid outlet (51) is opened on the side of the anti-drip plate (5) away from the connecting shaft (10). A sealing plate (91) is attached to the top of the waste liquid outlet (51). The sealing plate (91) seals the waste liquid outlet (51). The sealing plate (91) is installed with the waste liquid collection frame (9) through the mounting assembly (11). The tilting of the anti-drip plate (5) opens the waste liquid outlet (51).

2. The integrated gene extraction and amplification detection device according to claim 1, characterized in that: The drive assembly (6) includes a mounting plate (61), a drive cylinder (62), a mounting side plate (63), a support base plate (64), and a limiting side plate (65). The mounting plate (61) is slidably connected to the top of the main body (1) of the detection equipment, and the drive cylinder (62) is fixedly installed at the bottom of the mounting plate (61).

3. The integrated gene extraction and amplification detection device according to claim 2, wherein: The output end of the drive cylinder (62) is fixedly installed on the mounting side plate (63), the support base plate (64) is fixedly installed on the bottom side of the mounting side plate (63), the limiting side plate (65) is fixedly installed on the top side of the support base plate (64), and the waste liquid collection frame (9) is slidably connected between the support base plate (64) and the anti-drip plate (5).

4. The integrated gene extraction and amplification detection device according to claim 3, wherein: A micro motor (101) is fixedly installed on one side of the outer wall of the mounting side plate (63). The output end of the micro motor (101) is fixedly connected to one end of the connecting shaft (10), and the two ends of the connecting shaft (10) are rotatably connected to the mounting side plate (63).

5. The integrated gene extraction and amplification detection device of claim 1, wherein: The cleaning assembly (8) includes a disinfectant tank (81), a high-pressure nozzle pipe (82), and a cleaning scraper (83). The disinfectant tank (81) is fixedly installed on the outer wall of the mounting side plate (63), and the high-pressure nozzle pipe (82) is fixedly installed at one end inside the mounting frame (7).

6. The integrated gene extraction and amplification detection device according to claim 5, wherein: The high-pressure nozzle pipe (82) is connected to the disinfectant tank (81) via a connecting hose. The cleaning scraper (83) is slidably connected inside the mounting frame (7). The bottom of the cleaning scraper (83) is attached to the top of the anti-drip plate (5).

7. The integrated gene extraction and amplification detection device according to claim 1, characterized in that: The mounting assembly (11) includes a support rod (111), a threaded component (112), a rubber pad (113), a threaded cylinder (114), and a threaded ring (115). The top end of the support rod (111) passes through the waste liquid outlet (51) and is fixedly installed on both sides of the bottom of the sealing plate (91). The threaded cylinder (114) is located on both sides of the bottom of the waste liquid collection frame (9).

8. The integrated gene extraction and amplification detection device of claim 7, wherein: The threaded component (112) is fixedly installed at the bottom of the support rod (111). The bottom end of the threaded component (112) passes through the waste liquid collection frame (9) and is threadedly connected to the threaded cylinder (114). The threaded ring (115) is fixedly installed at the top of the threaded cylinder (114). The threaded ring (115) is threadedly connected to the bottom of the waste liquid collection frame (9). The rubber pad (113) is fixedly sleeved on the outer peripheral wall of the support rod (111). The rubber pad (113) is attached to the top of the waste liquid collection frame (9).

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