Cell culture constant flow liquid supplement device

Abstract

This invention provides a constant-flow nutrient solution replenishment device for cell culture, relating to the field of cell culture technology. It includes a base, an internally connected storage tank, a top-mounted lid, a fixedly connected inlet cylinder at the bottom of the storage tank, and an operating box fixedly connected to the top of the inlet cylinder. A constant-flow component is mounted on the outer surface of the operating box, comprising a drive unit and an output rod fixedly connected to the output end of the drive unit. This invention features a base supporting the storage tank, facilitating the flow of nutrient solution from the bottom inlet hole into the inlet cylinder. The inlet cylinder and guide plate are designed to introduce the nutrient solution into the culture chamber. The dust cover prevents dust from falling into the culture chamber during the nutrient solution's flow. The operating box serves the same purpose as the dust cover and also houses a constant-flow component for controlling the constant flow of the nutrient solution.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a constant flow replenishment device for cell culture. Background Technology

[0002] In cutting-edge fields such as biomedical research, drug development, and cell therapy, cell culture technology is an indispensable basic method. As a core device for maintaining the cell growth environment, the performance of cell culture replenishment devices directly affects experimental results and application effectiveness. However, existing cell culture replenishment devices generally have some unavoidable defects. Traditional replenishment devices rarely deliver stable and constant flow of nutrient solution. During cell culture, fluctuations in nutrient solution flow can adversely affect the cell growth environment, leading to unstable cell growth and even affecting cell activity and the accuracy of experimental results. Utility Model Content

[0003] The purpose of this invention is to address the shortcomings of existing technologies, such as the fact that traditional fluid replenishment devices rarely deliver nutrient solutions stably, and to provide a constant-flow fluid replenishment device for cell culture.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a cell culture constant flow replenishment device, comprising a base, a storage tank being snapped into the interior of the base, a lid being snapped into the top of the storage tank, an inlet cylinder being fixedly connected to the bottom of the storage tank, an operating box being fixedly connected to the top of the inlet cylinder, a constant flow component being provided on the outer surface of the operating box, the constant flow component comprising a drive body, an output rod being fixedly connected to the output end of the drive body, a turntable being fixedly connected to the top of the output rod, a constant flow hole being provided on the outer surface of the turntable, a dust cover being fixedly connected to the outer surface of the operating box, a culture chamber being fixedly connected to the top of the dust cover, and an inlet hole being provided on the inner wall of the storage tank.

[0005] In a preferred embodiment, the outer surface of the bucket lid is provided with a sealing assembly, the sealing assembly includes a support plate, a cover plate is provided on one side of the support plate, a moving groove is formed on the outer surface of the support plate, and a spring is fixedly connected to the inner wall of the moving groove.

[0006] In a preferred embodiment, a connecting plate is fixedly connected to one side of the cover plate, the outer surface of the connecting plate is fixedly connected to the top of the spring, and the cover plate is fitted to the top of the cover hole.

[0007] In a preferred embodiment, a support column is fixedly connected to the inner wall of the operating box, a diversion plate is fixedly connected to the top of the support column, a rotating groove is provided on the outer surface of the operating box, a liquid outlet is provided on the side of the operating box near the dust cover, and a cover hole is provided on the outer surface of the bucket lid.

[0008] In a preferred embodiment, the drainage plate is disposed on the lower side of the liquid inlet cylinder, and the drainage plate extends through the liquid outlet hole into the interior of the dust cover and the culture chamber.

[0009] In a preferred embodiment, the outer surface of the turntable is fitted to the inner wall of the rotating groove.

[0010] In a preferred embodiment, the liquid inlet cylinder is fixedly connected to the bottom of the liquid inlet hole.

[0011] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0012] This utility model features a base to support the storage tank, allowing the nutrient solution inside to flow downwards from the bottom inlet into the inlet cylinder. The inlet cylinder and the guide plate are both designed to introduce the nutrient solution into the culture chamber. The dust cover prevents dust from falling into the nutrient solution during its flow into the culture chamber. The operating box serves the same purpose as the dust cover, and also houses a constant flow component that controls the constant flow of the nutrient solution, thus ensuring a continuous constant flow of the nutrient solution. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of a cell culture constant flow replenishment device provided by the present invention.

[0014] Figure 2 This is a top view of a cell culture constant flow replenishment device provided by this utility model.

[0015] Figure 3 A cross-sectional schematic diagram of the operating box of a cell culture constant flow replenishment device provided by this utility model.

[0016] Figure 4 A schematic diagram of the internal structure of the housing of a cell culture constant flow replenishment device provided by this utility model.

[0017] Figure 5 This is a schematic diagram of the cover plate assembly of a cell culture constant flow replenishment device provided by this utility model.

[0018] Legend:

[0019] 1. Base; 2. Storage tank; 3. Tank lid; 4. Liquid inlet cylinder; 5. Operating box; 6. Constant flow assembly; 61. Drive body; 62. Output rod; 63. Turntable; 64. Constant flow orifice; 7. Dust cover; 8. Culture chamber; 9. Sealing assembly; 91. Support plate; 92. Cover plate; 93. Moving trough; 94. Spring; 10. Liquid inlet hole; 11. Support column; 12. Drain plate; 13. Rotating trough; 14. Liquid outlet hole; 15. Cover hole. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Example 1

[0022] like Figures 1-4 As shown, this utility model provides a technical solution: a cell culture constant flow replenishment device, including a base 1, a storage tank 2 is internally engaged with the base 1, a tank cover 3 is engaged with the top of the storage tank 2, an inlet cylinder 4 is fixedly connected to the bottom of the storage tank 2, the inlet cylinder 4 is fixedly connected to the bottom of the inlet hole 10, an operating box 5 is fixedly connected to the top of the inlet cylinder 4, a constant flow component 6 is provided on the outer surface of the operating box 5, the constant flow component 6 includes a drive body 61, an output rod 62 is fixedly connected to the output end of the drive body 61, a turntable 63 is fixedly connected to the top of the output rod 62, and the outer surface of the turntable 63 is attached to the rotating groove 13. The inner wall of the turntable 63 has a constant flow hole 64 on its outer surface. The outer surface of the operating box 5 is fixedly connected to a dust cover 7. The top of the dust cover 7 is fixedly connected to a culture chamber 8. The inner wall of the storage tank 2 has a liquid inlet hole 10. The inner wall of the operating box 5 is fixedly connected to a support column 11. The top of the support column 11 is fixedly connected to a flow guide plate 12. The flow guide plate 12 is located on the lower side of the liquid inlet cylinder 4. The flow guide plate 12 extends through the liquid outlet hole 14 into the interior of the dust cover 7 and the culture chamber 8. The outer surface of the operating box 5 has a rotating groove 13. The side of the operating box 5 near the dust cover 7 has a liquid outlet hole 14. The outer surface of the tank cover 3 has a cover hole 15.

[0023] In this embodiment, a base 1 is provided to support the storage tank 2, so that the nutrient solution in the tank can flow down from the bottom inlet hole 10 into the inlet cylinder 4. The inlet cylinder 4 and the guide plate 12 are both provided to introduce the nutrient solution into the culture chamber 8. The dust cover 7 is to prevent dust from falling into the nutrient solution during the flow into the culture chamber 8. The operation box 5 is provided, which serves the same purpose as the dust cover 7. On the other hand, a constant flow component 6 is provided inside the operation box 5 to control the constant flow of the nutrient solution. The output end of the drive body 61 in the constant flow component 6 passes through the through hole and is connected to the output rod 62 to drive the turntable 63 to rotate. The constant flow hole 64 is opened on the surface of the turntable 63. Liquid can only flow out when the constant flow hole 64 rotates to the outlet of the inlet cylinder 4, thereby achieving constant flow. The nutrient solution flows to the guide plate 12 and then flows into the culture chamber 8 through the outlet hole 14.

[0024] Example 2

[0025] like Figure 5 As shown, a sealing assembly 9 is provided on the outer surface of the bucket lid 3. The sealing assembly 9 includes a support plate 91. A cover plate 92 is provided on one side of the support plate 91. A moving groove 93 is opened on the outer surface of the support plate 91. A spring 94 is fixedly connected to the inner wall of the moving groove 93. A connecting plate is fixedly connected to one side of the cover plate 92. The outer surface of the connecting plate is fixedly connected to the top of the spring 94. The cover plate 92 fits against the top of the cover hole 15.

[0026] In this embodiment, a capping component 9 is provided. After the nutrient solution is added to the storage tank 2, it can automatically seal the cap hole 15. When opening, simply push the small protrusion on the outer surface of the cap plate 92 backward to move the cap plate 92 into the moving groove 93. The spring 94 is compressed. After the addition is completed, the support releases the small protrusion to put the cap plate 92 back on the surface of the cap hole 15.

[0027] Working principle:

[0028] like Figures 1-5 As shown, when the equipment is in use, the drive body 61 will be activated. The output end of the drive body 61 in the constant flow assembly 6 will pass through the operating box 5 and connect to the output rod 62, thereby driving the turntable 63 to rotate. The constant flow hole 64 on the surface of the turntable 63 can only discharge liquid when the constant flow hole 64 rotates to the outlet of the liquid inlet cylinder 4, thereby achieving constant flow. The nutrient liquid flows to the guide plate 12 and then flows into the culture chamber 8 through the liquid outlet hole 14. When replenishing the nutrient liquid in the storage tank 2, the small protrusion on the outer surface of the cover plate 92 is pulled back to move the cover plate 92 into the moving groove 93. The spring 94 is compressed. After the addition is completed, the support is released and the small protrusion can be moved back onto the surface of the cover hole 15.

[0029] The above are merely preferred embodiments of this utility model and are not intended to limit the utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model without departing from the technical solution of this utility model shall still fall within the protection scope of this utility model.

Claims

1. A cell culture constant flow replenishment device, comprising a base (1), characterized in that: The base (1) is internally connected to a storage tank (2), the top of the storage tank (2) is connected to a lid (3), the bottom of the storage tank (2) is fixedly connected to an inlet cylinder (4), the top of the inlet cylinder (4) is fixedly connected to an operating box (5), the outer surface of the operating box (5) is provided with a constant flow component (6), the constant flow component (6) includes a drive body (61), the output end of the drive body (61) is fixedly connected to an output rod (62), the top of the output rod (62) is fixedly connected to a turntable (63), the outer surface of the turntable (63) is provided with a constant flow hole (64), the outer surface of the operating box (5) is fixedly connected to a dust cover (7), the top of the dust cover (7) is fixedly connected to a culture chamber (8), and the inner wall of the storage tank (2) is provided with an inlet hole (10).

2. The cell culture constant flow replenishment device according to claim 1, characterized in that: The outer surface of the bucket lid (3) is provided with a sealing assembly (9), the sealing assembly (9) includes a support plate (91), a cover plate (92) is provided on one side of the support plate (91), a moving groove (93) is opened on the outer surface of the support plate (91), and a spring (94) is fixedly connected to the inner wall of the moving groove (93).

3. The cell culture constant flow replenishment device according to claim 2, characterized in that: A connecting plate is fixedly connected to one side of the cover plate (92), and the outer surface of the connecting plate is fixedly connected to the top of the spring (94). The cover plate (92) is attached to the top of the cover hole (15).

4. The cell culture constant flow replenishment device according to claim 1, characterized in that: The inner wall of the operating box (5) is fixedly connected to a support column (11), the top of the support column (11) is fixedly connected to a flow guide plate (12), the outer surface of the operating box (5) is provided with a rotating groove (13), the side of the operating box (5) near the dust cover (7) is provided with a liquid outlet hole (14), and the outer surface of the bucket cover (3) is provided with a cover hole (15).

5. A cell culture constant flow replenishment device according to claim 4, characterized in that: The flow guide plate (12) is located on the lower side of the liquid inlet cylinder (4), and the flow guide plate (12) extends through the liquid outlet hole (14) into the interior of the dust cover (7) and the culture chamber (8).

6. The cell culture constant flow replenishment device according to claim 1, characterized in that: The outer surface of the turntable (63) is attached to the inner wall of the rotating groove (13).

7. A cell culture constant flow replenishment device according to claim 1, characterized in that: The liquid inlet cylinder (4) is fixedly connected to the bottom of the liquid inlet hole (10).

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