A melon interspecific hybrid tissue culture seedling domestication device
By using a double-layer light-transmitting cover, an intelligent temperature and humidity system, and a zoned ventilation design, the problem of uneven environmental control and nutrient supply in the melon interspecific hybrid tissue culture seedling acclimatization device was solved, thereby improving the survival rate and breeding efficiency of the tissue culture seedlings.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HAINAN UNIV
- Filing Date
- 2025-07-30
- Publication Date
- 2026-06-26
AI Technical Summary
Existing melon interspecific hybridization tissue culture seedling domestication devices have shortcomings in terms of insufficient precision in environmental control, uneven nutrient supply, and low space utilization, resulting in high seedling mortality and low breeding efficiency.
It adopts a double-layer light-transmitting supplementary light cover and an intelligent temperature and humidity feedback system, combined with an M-shaped cotton thread liquid absorption structure and a zoned ventilation adjustment valve to achieve precise environmental control, balanced nutrient supply, and independent seedling area management.
It significantly improved the survival rate and breeding efficiency of tissue culture seedlings, reduced labor intensity and equipment costs, and met the needs of interspecific hybridization of melons under different environmental conditions.
Smart Images
Figure CN224402483U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the technical field of plant seedling domestication devices, and in particular relates to a domestication device for interspecific hybrid tissue culture seedlings of melon. Background Technology
[0002] Interspecific hybridization breeding of melons is an important way to overcome the limitations of germplasm resources and cultivate new stress-resistant varieties. However, the environmental adaptability and nutrient supply issues during the acclimatization stage of tissue culture seedlings have long constrained its industrial application. Existing research shows that interspecific hybrid tissue culture seedlings of melons are significantly more sensitive to light, temperature, and humidity than ordinary varieties. Traditional acclimatization devices, due to their extensive environmental control and unbalanced nutrient supply, result in a seedling mortality rate as high as 40%-60%, becoming a key bottleneck restricting breeding efficiency.
[0003] The existing technology has three major drawbacks: First, the environmental control precision is insufficient. The single-layer acclimatization cover has poor heat preservation performance, and the temperature difference between day and night exceeds 10°C. Ventilation control relies on manually opening and closing windows, and the humidity difference within the same device can reach more than 30%, making it difficult to meet the microenvironment consistency requirements of interspecific hybrids. Second, the nutrient supply system is inefficient. The single layout of the siphon-type cotton thread leads to nutrient deficiency at the edge of the substrate, and the lack of drainage structure causes water accumulation and root rot, with a root rot rate as high as 25%-35%. Third, the space utilization rate and ease of operation are poor. The seedling density per unit area of the integrated acclimatization box is low (≤50 seedlings / m²), and cross-contamination is easy when different varieties are mixed. Manual adjustment of parameters takes more than 2 hours per batch, which is labor-intensive and prone to introducing errors.
[0004] To address the aforementioned problems, this utility model proposes a novel device for the domestication of interspecific hybrid tissue culture seedlings of melon. Utility Model Content
[0005] The purpose of this invention is to provide a device for the domestication of interspecific hybrid tissue culture seedlings of melon, which achieves precise environmental control through a double-layer light-transmitting supplementary light cover and an intelligent temperature and humidity feedback system, achieves balanced nutrient supply through an M-shaped cotton thread liquid absorption structure combined with micro-circulation filtration, and achieves independent environmental management through a zoned ventilation adjustment valve.
[0006] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:
[0007] This utility model relates to a device for acclimatizing interspecific hybrid tissue culture seedlings of melon, comprising a nutrient solution tank, a substrate tank, a double-layer light-transmitting supplementary light cover, and a ventilation adjustment component; the substrate tank is located inside the nutrient solution tank, and the ventilation adjustment component is installed on the nutrient solution tank and the substrate tank; the ventilation adjustment component divides the substrate tank into multiple seedling acclimatization zones, and each seedling acclimatization zone is equipped with one of the double-layer light-transmitting supplementary light covers.
[0008] As a preferred embodiment of this utility model, the double-layer light-transmitting supplementary light cover includes a first rectangular ring plate; an inner arch cover and an outer arch cover are fixed on the upper surface of the first rectangular ring plate; the two ends of the inner arch cover and the outer arch cover are respectively sealed by an arched plate; a supplementary light is installed on the outer wall of the outer arch cover; a control switch is installed on the outer wall of the inner arch cover; an electric heating film is installed on the outer wall of the outer arch cover; a temperature sensor is installed through one of the arched plates; a temperature controller electrically connected to the electric heating film and the temperature sensor is installed on the outer wall of the outer arch cover; a first rectangular frame plate is fixed at the lower end of the first rectangular ring plate.
[0009] As a preferred technical solution of this utility model, the nutrient solution tank includes a rectangular long tank with an opening at the top and a larger top and smaller bottom; a rectangular ring step is provided on the inner wall of the upper end of the rectangular long tank; a row of first vent holes is provided on the side wall of the rectangular ring step along its length.
[0010] As a preferred embodiment of this utility model, the substrate tank includes a second rectangular ring plate; an arc-shaped elongated tank is fixed at the lower center of the second rectangular ring plate; the sidewall of the arc-shaped elongated tank has several pairs of first circular through holes along its length; a cotton thread is installed through each pair of first circular through holes; the cotton thread is arranged in an M-shape; a row of second circular through holes is opened at the bottom of the arc-shaped elongated tank; filter cotton segments are installed on the second circular through holes.
[0011] As a preferred embodiment of this utility model, the ventilation adjustment component includes a partition frame and several pairs of ventilation adjustment valves; the partition frame includes a third rectangular ring plate that cooperates with the second rectangular ring plate; several partition plates are fixed between the inner walls of the third rectangular ring plate along its length to divide the interior of the third rectangular ring plate into multiple seedling acclimatization zones, and the lower ends of the partition plates extend to the bottom of the third rectangular ring plate; each seedling acclimatization zone corresponds to a pair of rectangular first rectangular ventilation channels symmetrically opened at the lower end of the third rectangular ring plate.
[0012] As a preferred technical solution of this utility model, the ventilation adjustment valve includes a rectangular slider that is slidably installed between the third rectangular ring plate and the side wall of the rectangular ring step; the lower end of the rectangular slider is provided with a second rectangular ventilation channel that cooperates with the first rectangular ventilation channel and the first vent hole; an identification limiting baffle that passes through the first vent hole is fixed to the inner wall of one end of the second rectangular ventilation channel; and a handle is fixed to the outer side of the rectangular slider.
[0013] This utility model has the following beneficial effects:
[0014] 1. This utility model features a double-layer light-transmitting supplementary lighting cover with an inner and outer arched structure. Combined with a supplementary lighting lamp, an electric heating film, and a temperature sensor, it achieves precise control of light intensity, temperature, and humidity. The temperature controller automatically adjusts the operation of the electric heating film based on sensor feedback, avoiding stress damage to tissue culture seedlings caused by temperature fluctuations and significantly improving the survival rate of young plants in the early stages of acclimatization.
[0015] 2. In this utility model, the partition frame divides the substrate tank into independent seedling areas, each equipped with an independent ventilation adjustment valve. The ventilation volume is adjusted by sliding a rectangular slider, precisely controlling the gas exchange rate. This zoned management method avoids the extensive "all-on" approach of traditional devices and is particularly suitable for interspecific hybrid tissue culture seedlings that are sensitive to the environment.
[0016] 3. The M-shaped cotton thread at the bottom of the substrate trough in this invention continuously absorbs the nutrient solution through capillary action and diffuses it throughout the entire arc-shaped trough, ensuring uniform nutrient distribution in the substrate. Compared to traditional siphon strips, the M-shaped structure increases the absorption area, reducing the risk of localized excessively high or low nutrient solution concentrations. The second circular through-hole at the bottom of the arc-shaped trough, in conjunction with the filter cotton section, allows excess water to permeate back into the nutrient solution trough, forming a closed-loop system of "absorption-diffusion-filtration-recirculation." This design prevents waterlogging and root rot in the substrate and avoids nutrient solution waste, meeting the needs of water-saving agriculture.
[0017] 4. This utility model divides a single device into multiple seedling cultivation areas using partition frames, allowing for the simultaneous acclimatization of tissue culture seedlings of different varieties or growth stages. This design reduces the equipment's footprint and lowers the acclimatization cost per unit area, making it particularly suitable for laboratory or small-scale breeding scenarios. The nutrient solution tank, substrate tank, and double-layer light-transmitting supplemental lighting cover are connected by snap-fit mechanisms, making installation and disassembly convenient. The number of seedling cultivation areas can be adjusted according to actual needs, avoiding the drawback of traditional integrated devices where "one failure renders the entire device useless."
[0018] 5. The combination of the electric heating film and the supplemental lighting in this invention enables the device to operate normally in low-temperature or low-light environments, breaking through the dependence of traditional devices on environmental conditions. By adjusting the ventilation volume, light intensity, and temperature parameters, the device can be adapted to the domestication needs of different interspecific hybrids of melons, providing a standardized technical platform for genetic improvement research.
[0019] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description
[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0021] Figure 1 This is a schematic diagram of the structure of the melon interspecific hybrid tissue culture seedling domestication device of this utility model.
[0022] Figure 2 for Figure 1 A schematic diagram of the transverse cross section.
[0023] Figure 3 This is a schematic diagram of the nutrient solution tank.
[0024] Figure 4 This is a schematic diagram of the substrate tank.
[0025] Figure 5 This is a schematic diagram of a double-layer light-transmitting supplementary light cover.
[0026] Figure 6 This is a schematic diagram of the separator frame.
[0027] Figure 7 This is a schematic diagram of the ventilation regulating valve.
[0028] The attached diagram lists the components represented by each number as follows:
[0029] 1-Nutrient solution tank, 2-Substrate tank, 3-Double-layer light-transmitting supplementary light cover, 31-First rectangular ring plate, 32-Inner arch cover, 33-Outer arch cover, 34-Arch plate, 35-Supplementary light, 36-Control switch, 37-Electric heating film, 38-Temperature sensor, 39-Temperature controller, 310-First rectangular frame plate, 11-Rectangular long groove, 12-Rectangular ring step, 13-First vent hole, 21-Second rectangular ring plate, 22-Arc-shaped long groove, 23-First round through hole, 24-Cotton thread, 25-Second round through hole, 26-Filter cotton section, 4-Separator frame, 5-Ventilation adjustment valve, 41-Third rectangular ring plate, 42-Separator plate, 43-First rectangular ventilation channel, 51-Rectangular slider, 52-Second rectangular ventilation channel, 53-Identification limit baffle, 54-Handle lever. Detailed Implementation
[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model. Specific Implementation Example 1:
[0032] Please see Figure 1-7 As shown, this utility model is a device for acclimatizing interspecific hybrid tissue culture seedlings of melon, including a nutrient solution tank 1, a substrate tank 2, a double-layer light-transmitting supplementary lighting cover 3, and a ventilation adjustment component. The substrate tank 2 is installed inside the nutrient solution tank 1, and the ventilation adjustment component is installed on the nutrient solution tank 1 and the substrate tank 2. The ventilation adjustment component divides the substrate tank 2 into multiple seedling acclimatization zones, and each seedling acclimatization zone is equipped with a double-layer light-transmitting supplementary lighting cover 3.
[0033] The double-layer light-transmitting supplementary lighting cover 3 includes a first rectangular ring plate 31. A double-layered inner arch cover 32 and an outer arch cover 33 are fixed to the upper surface of the first rectangular ring plate 31. The inner arch cover 32 and the outer arch cover 33 are sealed at both ends by an arched plate 34. A supplementary lighting lamp 35 is installed on the outer wall of the outer arch cover 33 to provide necessary lighting conditions for the tissue culture seedlings. A control switch 36 for controlling the supplementary lighting lamp 35 is installed on the outer wall of the inner arch cover 32, allowing users to manually control the lighting environment inside the cover. An electric heating film 37 is installed on the outer wall of the outer arch cover 33 to heat the air inside the cover. A temperature sensor 38 is installed through one of the arched plates 34 to monitor the temperature inside the cover in real time. A temperature controller 39, electrically connected to the electric heating film 37 and the temperature sensor 38, is installed on the outer wall of the outer arch cover 33. Based on feedback from the temperature sensor 38, the controller automatically adjusts the working state of the electric heating film 37 to maintain a stable temperature inside the cover. The lower end of the first rectangular ring plate 31 is fixed with a first rectangular frame plate 310 to enhance structural stability, and the first rectangular frame plate 310 is used to insert and install on the partition frame 4 to improve sealing. The double-layer light-transmitting supplementary lighting cover 3 has significantly improved heat preservation performance compared to the single-layer transparent cover, which helps to avoid rapid temperature changes inside the double-layer light-transmitting supplementary lighting cover 3. The double-layer light-transmitting supplementary lighting cover 3 has the functions of supplemental lighting and temperature control, which facilitates providing a stable light and temperature environment for seedling acclimatization over a long period of time.
[0034] The nutrient solution tank 1 includes a rectangular elongated tank 11 that is open at the top and wider at the bottom than at the top. A rectangular ring step 12 is formed on the inner wall of the upper end of the rectangular elongated tank 11 to support the substrate tank 2. A row of first ventilation holes 13 are formed on the side walls along the length of the rectangular ring step 12, which are used in conjunction with the ventilation adjustment component to adjust the ventilation volume inside the hood.
[0035] The substrate tank 2 includes a second rectangular ring plate 21. An arc-shaped elongated trough 22 is fixed to the lower center of the second rectangular ring plate 21 to support the culture substrate. Several pairs of first circular through-holes 23 are formed along the length of the sidewall of the arc-shaped elongated trough 22. A cotton thread 24 is threaded through each pair of first circular through-holes 23. The cotton thread 24 is arranged in an M-shape to increase the absorption area and effectively absorb nutrient solution from the nutrient solution tank 1 and diffuse it into the substrate. A row of second circular through-holes 25 is formed at the bottom of the arc-shaped elongated trough 22. Filter cotton segments 26 are installed on the second circular through-holes 25. The M-shaped cotton thread 24 in the substrate tank 2 facilitates the absorption of nutrient solution from the nutrient solution tank 1 and its wide diffusion into the substrate within the arc-shaped elongated trough 22. Simultaneously, the filter cotton segments 26 installed at the bottom of the arc-shaped elongated trough 22 allow excess water in the substrate to drip back into the nutrient solution tank 1 below, forming a micro-circulation of the nutrient solution and ensuring the stability of the moisture in the substrate.
[0036] The ventilation adjustment assembly includes a partition frame 4 and several pairs of ventilation adjustment valves 5. The partition frame 4 includes a third rectangular ring plate 41 that cooperates with the second rectangular ring plate 21. Several partition plates 42 are fixed between the inner walls along the length of the third rectangular ring plate 41, dividing the interior of the third rectangular ring plate 41 into multiple seedling acclimatization zones. The lower ends of the partition plates 42 extend below the third rectangular ring plate 41 to enhance the separation effect. Each seedling acclimatization zone has a pair of rectangular ventilation channels 43 symmetrically opened at the lower end of the third rectangular ring plate 41 for gas exchange.
[0037] The ventilation regulating valve 5 includes a rectangular slider 51 slidably mounted between the third rectangular ring plate 41 and the side wall of the rectangular ring step 12. A second rectangular ventilation channel 52, which mates with the first rectangular ventilation channel 43 and the first vent hole 13, is provided at the lower end of the rectangular slider 51. An identification limiting baffle 53, passing through the first vent hole 13, is fixed to the inner wall of one end of the second rectangular ventilation channel 52 to limit the sliding range of the rectangular slider 51. A handle 54 is fixed to the outer side of the rectangular slider 51 for easy operation. By squeezing the handle 54, the entire ventilation regulating valve 5 is moved between the third rectangular ring plate 41 and the side wall of the rectangular ring step 12, thereby adjusting the communication area between the second rectangular ventilation channel 52, the first rectangular ventilation channel 43, and the first vent hole 13, thus achieving ventilation within the double-layer light-transmitting supplementary light cover 3 and adjusting the humidity within the double-layer light-transmitting supplementary light cover 3.
[0038] A specific application of this embodiment is as follows: When using the novel melon interspecific hybrid tissue culture seedling acclimatization device, firstly, the substrate, scalpels, and plates to be used are placed in a high-temperature sterilizer for high-temperature sterilization. After sterilization, they are placed together with sterile water, nutrient solution, and washed pots and containers in a clean bench and irradiated with ultraviolet light for 30 minutes. A portion of the sterilized substrate is first placed in substrate tank 2. Then, using sterilized and cooled scalpels, the tissue culture seedlings are removed from the tissue culture bottles and their roots are cleaned in sterile water. The seedlings are then placed in the cultivation structure using large tweezers, substrate is added again and compacted, and then an appropriate amount of nutrient solution is poured in to promote close contact between the substrate and roots, facilitating plant growth. Nutrient solution is added to nutrient solution tank 1, ensuring the liquid level is appropriate. Then, substrate tank 2 is installed inside nutrient solution tank 1, ensuring that the cotton thread 24 is fully in contact with the nutrient solution. Subsequently, the partition frame 4 and several pairs of ventilation adjustment valves 5 are installed. Finally, multiple double-layered light-transmitting supplementary lighting covers 3 are installed, and the ventilation adjustment valves 5 are adjusted to provide a suitable ventilation volume, providing a stable light, temperature, and humidity environment for the tissue culture seedlings. During the acclimatization process, the light intensity of the supplementary lighting lamps 35 and the working status of the electric heating film 37 are adjusted in a timely manner according to the growth of the tissue culture seedlings to ensure healthy growth. When the tissue culture seedlings have grown to a suitable age for transplanting, the corresponding double-layered light-transmitting supplementary lighting covers 3 are removed, and the seedlings are taken out of the acclimatization device and transplanted to the field or greenhouse for continued cultivation.
[0039] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.
[0040] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.
Claims
1. A device for acclimatizing interspecific hybrid tissue culture seedlings of melon, characterized in that: It includes a nutrient solution tank (1), a substrate tank (2), a double-layer light-transmitting supplementary light cover (3), and a ventilation adjustment component; The substrate tank (2) is located inside the nutrient solution tank (1), and the ventilation adjustment assembly is installed on the nutrient solution tank (1) and the substrate tank (2); The ventilation adjustment component divides the substrate tank (2) into multiple seedling acclimatization zones, and each seedling acclimatization zone is equipped with a double-layer light-transmitting supplementary light cover (3).
2. The melon interspecific hybrid tissue culture seedling acclimatization device according to claim 1, characterized in that, The double-layer light-transmitting supplementary light cover (3) includes a first rectangular ring plate (31); a double-layered inner arch cover (32) and an outer arch cover (33) are fixed on the upper surface of the first rectangular ring plate (31); the two ends of the inner arch cover (32) and the outer arch cover (33) are respectively sealed by an arched plate (34); a supplementary light (35) is installed on the outer wall of the outer arch cover (33); a control switch (36) is installed on the outer wall of the inner arch cover (32); an electric heating film (37) is installed on the outer wall of the outer arch cover (33); a temperature sensor (38) is installed through one of the arched plates (34); a temperature controller (39) electrically connected to the electric heating film (37) and the temperature sensor (38) is installed on the outer wall of the outer arch cover (33); a first rectangular frame plate (310) is fixed at the lower end of the first rectangular ring plate (31).
3. The melon interspecific hybrid tissue culture seedling acclimatization device according to claim 2, characterized in that, The nutrient solution tank (1) includes a rectangular long tank (11) with an open top and a larger top and smaller bottom; a rectangular ring step (12) is provided on the inner wall of the upper end of the rectangular long tank (11); a row of first ventilation holes (13) are provided on the side wall of the rectangular ring step (12) along its length.
4. The melon interspecific hybrid tissue culture seedling acclimatization device according to claim 3, characterized in that, The substrate tank (2) includes a second rectangular ring plate (21); an arc-shaped long groove (22) is fixed at the lower middle part of the second rectangular ring plate (21); the side wall of the arc-shaped long groove (22) has several pairs of first round through holes (23) along its length; a cotton thread (24) is installed through each pair of first round through holes (23); the cotton thread (24) is arranged in an M-shape; a row of second round through holes (25) is opened at the bottom of the arc-shaped long groove (22); filter cotton segments (26) are installed on the second round through holes (25).
5. The melon interspecific hybrid tissue culture seedling acclimatization device according to claim 4, characterized in that, The ventilation adjustment assembly includes a partition frame (4) and several pairs of ventilation adjustment valves (5); the partition frame (4) includes a third rectangular ring plate (41) that cooperates with the second rectangular ring plate (21); several partition plates (42) are fixed between the inner walls of the third rectangular ring plate (41) along its length to divide the interior of the third rectangular ring plate (41) into multiple seedling acclimatization zones, and the lower end of the partition plate (42) extends to the bottom of the third rectangular ring plate (41); each seedling acclimatization zone corresponds to a pair of rectangular first rectangular ventilation channels (43) symmetrically opened at the lower end of the third rectangular ring plate (41).
6. The melon interspecific hybrid tissue culture seedling acclimatization device according to claim 5, characterized in that, The ventilation regulating valve (5) includes a rectangular slider (51) that is slidably installed between the third rectangular ring plate (41) and the side wall of the rectangular ring step (12); the lower end of the rectangular slider (51) is provided with a second rectangular ventilation channel (52) that cooperates with the first rectangular ventilation channel (43) and the first vent hole (13); an identification limiting baffle (53) that passes through the first vent hole (13) is fixed on the inner wall of one end of the second rectangular ventilation channel (52); and a handle (54) is fixed on the outer side of the rectangular slider (51).