A device for sterilizing leaves in a plant tissue

Abstract

The utility model relates to tissue culture technical field, concretely is a kind of plant tissue leaf disinfection device, including disinfection cup, the placing assembly for limiting leaf placement is equipped in disinfection cup, cup cover is inserted and is pulled in the top of disinfection cup, the centre position of cup cover is equipped with the stirring assembly for the eccentric stirring of the placing assembly, the top of stirring assembly is equipped with the driving assembly for the eccentric rotation of the stirring assembly;By setting driving assembly, stirring assembly and placing assembly, leaf is placed in filter box and sinks into disinfectant, uses stirring shaft to drive filter box eccentric stirring, generates multidirectional eddy current, makes leaf and disinfectant contact more fully, guarantees disinfection effect, simultaneously through filter box can quickly take out leaf, can avoid frequent damage leaf by clamping, after disinfection is completed, through identical stirring mode can make leaf and sterile water fully contact, effectively clean residual disinfectant, improve cleaning effect.

Description

Technical Field

[0001] This utility model relates to the field of tissue culture technology, and in particular to a disinfection device for leaves in plant tissues. Background Technology

[0002] The main purpose of leaf disinfection is to kill microorganisms attached to the leaf surface, including bacteria, fungi, and viruses, in order to prevent these microorganisms from contaminating the culture medium and plant tissue during plant tissue culture, thereby ensuring the success rate of plant tissue culture.

[0003] The commonly used disinfection method is to place the explant in a sterilization cup, pour in disinfectant alcohol, pour out the disinfectant alcohol after 30-40 seconds, then pour in sodium hypochlorite solution or mercuric chloride disinfectant, pour out the disinfectant after 10-20 minutes, and then rinse 3 times with sterile water.

[0004] A plant explant surface sterilization and filtration device disclosed in Chinese Patent Publication No. CN219305656U is described. This device allows for the replacement of filters with appropriate mesh sizes based on the size of the explant, enhancing adaptability and better meeting experimental requirements. However, according to related technologies and existing technologies, existing leaf disinfection devices typically involve immersing the leaves in a disinfectant, which makes it difficult for the leaves to fully contact the disinfectant, resulting in poor disinfection. Furthermore, after disinfection, the leaves need to be repeatedly rinsed in sterile water with tweezers, which is ineffective and can easily damage the leaves. Utility Model Content

[0005] The purpose of this invention is to overcome the shortcomings of the prior art, solve the problems mentioned in the background art, and provide a disinfection device for leaves in plant tissues.

[0006] The purpose of this utility model is achieved through the following technical solution: a disinfection device for leaves in plant tissue, including a disinfection cup, wherein a placement component for limiting the placement of leaves is provided inside the disinfection cup, a cup lid is plugged into the top of the disinfection cup, a stirring component for driving the placement component to eccentrically stir is provided at the center of the cup lid, a driving component for driving the stirring component to rotate eccentrically is provided at the top of the stirring component, and a support component for providing stable support for the disinfection cup is provided at the bottom of the disinfection cup.

[0007] Preferably, the stirring assembly includes a hinge fixed at the center of the cup lid, a hinge ball rotatably mounted at the center of the hinge, and an inclined swing shaft passing through the hinge ball.

[0008] Preferably, the drive assembly includes a mounting cover fixed to the top of the cup lid, a rotating shaft extending through the top of the mounting cover, a rotating handle fixed to the top of the rotating shaft, a turntable fixed to the bottom of the rotating shaft, and the top of the swing shaft eccentrically fixed to the bottom of the turntable.

[0009] Preferably, the placement assembly includes a fixed cover fixed to the bottom of the swing shaft, and a filter box is inserted and removed from the bottom of the fixed cover.

[0010] Preferably, the support assembly includes a base that is pluggably installed at the bottom of the sterilization cup, and a suction cup is fixedly provided on the top of the base.

[0011] Preferably, a rotating groove for mounting the hinge ball is provided at the center of the hinge seat.

[0012] Preferably, the filter box is a flat cuboid with a porous structure.

[0013] Beneficial effects:

[0014] This plant tissue leaf disinfection device, through the inclusion of a drive component, an agitation component, and a placement component, places the leaves in a filter box and immerses them in disinfectant. The agitation shaft drives the filter box to agitate eccentrically, generating multi-directional vortices that allow the leaves to fully rotate and contact the disinfectant, ensuring thorough contact and guaranteeing effective disinfection. Simultaneously, the filter box allows for quick removal of the leaves, avoiding frequent handling that could damage them. After disinfection, the same agitation method ensures the leaves are fully in contact with sterile water, effectively washing away residual disinfectant and improving the cleaning effect. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a schematic diagram showing the state of the present invention during disinfection;

[0018] Figure 3 This is a schematic diagram showing the position of the filter frame during disinfection according to this utility model;

[0019] Figure 4 This is a schematic diagram of the structure of the drive component of this utility model;

[0020] Figure 5 This is a schematic diagram of the agitation component of this utility model;

[0021] Figure 6 This is a schematic diagram showing the connection between the swing shaft and the turntable of this utility model.

[0022] In the diagram: 1. Sterilization cup; 2. Cup lid; 3. Agitator assembly; 31. Hinge seat; 32. Hinge ball; 33. Swing shaft; 4. Drive assembly; 41. Mounting cover; 42. Rotating shaft; 43. Turntable; 44. Rotating handle; 5. Placement assembly; 51. Fixing cover; 52. Filter box; 6. Support assembly; 61. Base; 62. Suction cup; 7. Rotating groove. Detailed Implementation

[0023] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0024] Additional aspects and advantages of this invention will be further set forth in the description which follows in conjunction with the accompanying drawings, and in part will be obvious from the description or may be learned by practice of the invention.

[0025] like Figures 1 to 6 As shown, a disinfection device for leaves in plant tissue includes a disinfection cup 1, a placement component 5 for positioning and holding the leaves inside the cup 1, a lid 2 pluggably mounted on the top of the cup 1, a stirring component 3 for eccentrically agitating the placement component 5 at the center of the lid 2, a driving component 4 for eccentrically rotating the stirring component 3 at the top of the stirring component 3, and a support component 6 for providing stable support to the bottom of the cup 1. The disinfection cup 1 is fixed to a workbench by the support component 6. In use, disinfectant is poured into the cup 1, then the leaves are placed inside the placement component 5, and the lid 2 is closed. The cup lid 2 extends the placement component 5 into the sterilization cup 1 via the stirring component 3, immersing the blades in the disinfectant. At this time, the driving component 4 controls the eccentric rotation of the stirring component 3, causing the stirring component 3 to drive the blades in the placement component 5 to eccentrically stir in the disinfectant, making the blades more fully contacted with the disinfectant and ensuring the disinfection effect of the disinfectant on the blades. After disinfection, the cup lid 2 is opened and the blades are lifted out through the placement component 5 without the need to clamp the blades. Then, the disinfectant is poured out and sterile water is poured in. At this time, the above stirring process can make the blades fully contact with the sterile water, washing off the disinfectant on the blades and improving the cleaning effect of the sterile water on the blades.

[0026] like Figures 3 to 5 As shown, the stirring assembly 3 includes a hinge seat 31 fixed at the center of the cup lid 2. A hinge ball 32 is rotatably mounted at the center of the hinge seat 31. An inclined swing shaft 33 passes through the hinge ball 32. A rotating groove 7 for mounting the hinge ball 32 is opened at the center of the hinge seat 31. The blade is placed inside the placement assembly 5, and then the cup lid 2 is placed on the sterilization cup 1. At this time, the swing shaft 33 sinks the blade into the sterilization cup 1 through the placement assembly 5 at its bottom, so that the blade is immersed in the disinfectant in the sterilization cup 1. Then, the drive assembly 4 drives the top of the swing shaft 33 to rotate eccentrically, so that the bottom of the swing shaft 33 rotates eccentrically inside the sterilization cup 1 under the support of the hinge ball 32 and the hinge seat 31. The swing shaft 33 drives the blade inside the placement assembly 5 to stir eccentrically inside the sterilization cup 1, so that the blade and the disinfectant can be in more sufficient contact. The eccentric stirring setting can generate multi-directional vortex, so that the blade can be fully turned and contacted in the disinfectant, which promotes more sufficient contact between the blade and the disinfectant and ensures the disinfection effect.

[0027] like Figure 3 and Figure 4 As shown, the drive assembly 4 includes a mounting cover 41 fixed to the top of the cup lid 2. A rotating shaft 42 is provided through the top of the mounting cover 41. A rotating handle 44 is fixed to the top of the rotating shaft 42. A turntable 43 is fixed to the bottom of the rotating shaft 42. The top of the swing shaft 33 is eccentrically fixed to the bottom of the turntable 43. The rotating shaft 42 is rotated by the rotating handle 44, which causes the rotating shaft 42 to drive the turntable 43 to rotate. The turntable 43 then drives the top of the swing shaft 33 to rotate around the rotating shaft 42.

[0028] like Figure 1 and Figure 4 As shown, the placement component 5 includes a fixed cover 51 fixed to the bottom of the swing shaft 33. A filter box 52 is inserted and installed at the bottom of the fixed cover 51. The filter box 52 is a flat cuboid with a porous structure. The blade is placed into the flat filter box 52, and the top of the filter box 52 is inserted into the fixed cover 51 to limit the placement of the blade. After disinfection, the cup lid 2 can be opened to move the filter box 52 out of the disinfection cup 1, and the blade can be lifted out of the disinfection cup 1 through the filter box 52 without clamping the blade, thus reducing damage to the blade.

[0029] like Figure 1 As shown, the support component 6 includes a base 61 that is pluggably installed at the bottom of the sterilization cup 1, and a suction cup 62 is fixedly provided on the top of the base 61; the suction cup 62 is attached to the workbench to fix the base 61, and at the same time the bottom of the sterilization cup 1 is inserted into the base 61 to fix the sterilization cup 1, thereby ensuring the stability of the sterilization cup 1 when it is stirred.

[0030] The work process is as follows:

[0031] S1: As Figure 1As shown, during disinfection, the suction cup 62 is attached to the workbench to fix the base 61, and at the same time, the bottom of the disinfection cup 1 is inserted into the base 61 to fix the disinfection cup 1. After fixing, disinfectant is poured into the disinfection cup 1.

[0032] S2: As Figures 1 to 3 As shown, the blades are then placed into the flat filter box 52, and the top of the filter box 52 is inserted into the fixed cover 51 with the top facing upward. Then the cup lid 2 is placed on the disinfection cup 1. At this time, the swing shaft 33 sinks the filter box 52 into the disinfection cup 1 through the fixed cover 51, so that the blades are immersed in the disinfectant in the disinfection cup 1.

[0033] S3: As Figure 4 As shown, the rotating shaft 42 is then rotated by the rotating handle 44, causing the rotating shaft 42 to drive the rotating disk 43 to rotate. The rotating disk 43 then drives the top of the swing shaft 33 to rotate around the rotating shaft 42, thereby causing the filter box 52 at the bottom of the swing shaft 33 to rotate eccentrically inside the sterilization cup 1 under the support of the hinge ball 32 and the hinge seat 31.

[0034] S4: As Figure 4 As shown, the filter box 52 drives the blades to stir in the disinfectant, so that the blades and disinfectant can be in more thorough contact. The eccentric stirring setting can generate multi-directional vortex, so that the blades can be fully turned and contacted in the disinfectant, ensuring that the blades and disinfectant are in more thorough contact and disinfection effect. After stirring for a few rounds, let it stand for fifteen minutes for disinfection.

[0035] S5: As Figure 3 As shown, after disinfection, open the cup lid 2, so that the cup lid 2 moves the filter box 52 out of the disinfection cup 1, and the leaf is lifted out of the disinfection cup 1 through the filter box 52. There is no need to clamp the leaf, which reduces damage to the leaf.

[0036] S6: As Figure 4 As shown, the disinfectant in the disinfection cup 1 is then poured out and sterile water is poured in. At this time, the above stirring process can make the leaves come into full contact with the sterile water, wash off the disinfectant on the leaves, and ensure the cleaning effect of the sterile water on the leaves.

[0037] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A device for disinfecting leaves in plant tissue, characterized by: The cup includes a sterilization cup (1), which has a placement component (5) for limiting the placement of blades inside. A cup lid (2) is plugged into the top of the sterilization cup (1). A stirring component (3) for driving the placement component (5) to stir eccentrically is provided at the center of the cup lid (2). A driving component (4) for driving the stirring component (3) to rotate eccentrically is provided at the top of the stirring component (3). A support component (6) for providing stable support for the sterilization cup (1) is provided at the bottom of the sterilization cup (1).

2. A plant tissue leaf disinfestation apparatus as claimed in claim 1, wherein: The stirring assembly (3) includes a hinge (31) fixed at the center of the cup lid (2), and a hinge ball (32) is rotatably mounted at the center of the hinge (31), with an inclined swing shaft (33) passing through the hinge ball (32).

3. The disinfection device for leaves in plant tissue according to claim 2, characterized in that: The drive assembly (4) includes a mounting cover (41) fixed to the top of the cup lid (2), a rotating shaft (42) is provided through the top of the mounting cover (41), a rotating handle (44) is fixed to the top of the rotating shaft (42), a turntable (43) is fixed to the bottom of the rotating shaft (42), and the top of the swing shaft (33) is eccentrically fixed to the bottom of the turntable (43).

4. The disinfection device for leaves in plant tissue according to claim 3, characterized in that: The placement assembly (5) includes a fixing cover (51) fixed to the bottom of the swing shaft (33), and a filter box (52) is inserted and removed from the bottom of the fixing cover (51).

5. The disinfection device for leaves in plant tissue according to claim 1, characterized in that: The support assembly (6) includes a base (61) that is pluggable and installed at the bottom of the sterilization cup (1), and a suction cup (62) is fixedly provided on the top of the base (61).

6. The disinfection device for leaves in plant tissue according to claim 2, characterized in that: The hinge seat (31) has a rotating groove (7) at its center for mounting the hinge ball (32).

7. The disinfection device for leaves in plant tissue according to claim 4, characterized in that: The filter box (52) is a flat cuboid with a porous structure.

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