A sterile syringe type spleen lymphocyte separation and extraction device

Abstract

The utility model discloses a sterile needle cylinder type spleen lymphocyte separation and extraction device, including separation part, grinding part, collection part, and separation part is needle cylinder shape, including detachable connection's upper cylinder, lower cylinder, and the lower end detachable connection cell screen of upper cylinder, and the lower end detachable connection collection part of lower cylinder, and grinding part includes push rod, rocker, and the lower end of push rod is equipped with piston head, and piston head and upper cylinder closely slide and cooperate, and the center of push rod and piston head is equipped with the through -hole of continuity, and the rocker is slid and is arranged in the through -hole, and the upper end is stretched out push rod outside and is connected the rocking handle, and the lower end is stretched out piston head outside and is connected the grinding pestle, and the lateral wall of upper cylinder is equipped with liquid adding pipe, and one end of liquid adding pipe is located in the upper cylinder, and the other end is located in the upper cylinder outside. The utility model can carry out the coherent operation of organization grinding, cell release, impurity filtration, lymphocyte enrichment under the semi -closed environment, reduces the sample pollution risk, and promotes the repeatability of experiment.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a sterile syringe-type spleen lymphocyte separation and extraction device. Background Technology

[0002] Lymphocytes, white blood cells produced by lymphoid organs (including lymph nodes, spleen, and thymus), constitute a key component of the body's immune response. They are the main executors of almost all immune functions in the lymphatic system, acting as the frontline "warriors" against external infections and monitoring cellular mutations within the body. The spleen, as one of the key organs of the body's immune system, is rich in lymphocytes, accounting for a quarter of the total lymphoid tissue in the body. However, current equipment and technology are largely focused on medical research, particularly in techniques for extracting lymphocytes from blood, while research on spleen grinding in animal experiments is relatively lacking. In mouse experiments, due to the small blood volume, isolating large amounts of lymphocytes from peripheral blood is difficult; therefore, obtaining lymphocytes from the spleen is often necessary, making it an indispensable sample source for immunological research. The isolation and extraction of spleen lymphocytes is an essential foundational step in many fields, including immunological research, vaccine development, and disease diagnosis and treatment.

[0003] Traditional spleen lymphocyte isolation techniques primarily rely on manual operations, including steps such as mechanical grinding, enzymatic digestion, and density gradient centrifugation. Mechanical grinding involves repeatedly grinding spleen tissue in a mortar to release cells, while density gradient centrifugation utilizes the difference in sedimentation rates of different cellular components in a density gradient medium to achieve cell separation. These steps are fragmented and disjointed, relying on scattered consumables (such as centrifuge tubes, sieves, and grinding pestles), resulting in poor operational continuity, low efficiency, and susceptibility to microbial contamination in an open environment, affecting cell viability and experimental reproducibility. Utility Model Content

[0004] This invention provides a sterile syringe-type spleen lymphocyte separation and extraction device, which can perform continuous operations of tissue grinding, cell release, impurity filtration and lymphocyte enrichment in a semi-closed environment, reducing the risk of sample contamination and improving the reproducibility of experiments.

[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by this utility model is as follows:

[0006] A sterile syringe-type spleen lymphocyte separation and extraction device includes a separation section, a grinding section, and a collection section. The separation section is syringe-shaped and includes a detachably connected upper cylinder and a lower cylinder. A cell sieve is detachably connected to the lower end of the upper cylinder, and the collection section is detachably connected to the lower end of the lower cylinder. The grinding section includes a push rod and a rocker arm. A piston head is provided at the lower end of the push rod. The piston head is in close sliding fit with the upper cylinder. A continuous through hole is provided in the center of the push rod and the piston head. The rocker arm slides through the through hole, with its upper end extending out of the push rod and connected to a rocker handle, and its lower end extending out of the piston head and connected to a grinding pestle. A liquid addition tube is provided on the side wall of the upper cylinder, with one end of the liquid addition tube located inside the upper cylinder and the other end located outside the upper cylinder.

[0007] Furthermore, the lower cylinder is equipped with a cell filter, the pore size of which is smaller than that of a cell sieve.

[0008] Furthermore, the outer diameter of the cell sieve is smaller than the inner diameter of the upper and lower cylinders, and the top of the cell sieve is provided with a flange that is detachably connected to the upper cylinder.

[0009] Furthermore, the cell sieve is snapped or inserted into the upper cylinder.

[0010] Furthermore, the center line of the grinding pestle is parallel to but does not coincide with the center line of the rocker arm.

[0011] Furthermore, the minimum distance from the center of the rocker arm to the side of the grinding pestle is less than the distance from the center of the rocker arm to the inner end of the liquid adding tube.

[0012] Furthermore, the maximum distance from the bottom edge of the grinding pestle to the center of the rocker arm is less than the inner diameter of the cell sieve.

[0013] Furthermore, the upper cylinder and the lower cylinder are threaded together.

[0014] Furthermore, the collection section is a centrifuge tube, and the lower cylinder is connected to the centrifuge tube via a converter.

[0015] Furthermore, the outer end of the liquid addition tube is provided with a plug.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] This invention enables continuous operations of tissue grinding, cell release, impurity filtration, and lymphocyte enrichment in a semi-closed environment, reducing the risk of sample contamination and improving experimental reproducibility. It features a hand-cranked grinding pestle for gentler grinding, avoiding cell damage and enhancing cell viability. A double-layered filter allows for coarse and fine filtration, ensuring more thorough filtration. A syringe-type operating chamber generates negative pressure by pushing a push rod, facilitating rapid filtration of the cell separation solution. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a cross-sectional view showing the connection of the upper cylinder, cell sieve, and lower cylinder.

[0021] In the diagram: 1-Upper cylinder, 2-Lower cylinder, 3-Cell sieve, 31-Flange, 4-Push rod, 5-Rock bar, 6-Piston head, 7-Through hole, 8-Handle, 9-Grinding pestle, 10-Liquid addition tube, 11-Cell filter, 12-Center tube, 13-Converter, 14-Block. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0023] In the description of the embodiments of this application, it should be noted that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that the product of this application is usually placed in when in use, or the orientation or positional relationship that is commonly understood by those skilled in the art. It is only for the convenience of describing this application and simplifying the description, and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this application.

[0024] In the description of the embodiments of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set," "install," and "connect" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0025] like Figure 1As shown, a sterile syringe-type spleen lymphocyte separation and extraction device includes a separation section, a grinding section, and a collection section. The separation section is syringe-shaped and includes a detachably connected upper cylinder 1 and lower cylinder 2. Preferably, the upper cylinder 1 and lower cylinder 2 are threadedly connected. A cell sieve 3 is detachably connected to the lower end of the upper cylinder 1. A cell filter 11 is provided inside the lower cylinder 2, and the pore size of the cell filter 11 is smaller than that of the cell sieve 3. The outer diameter of the cell sieve 3 is smaller than the inner diameter of the upper cylinder 1 and the lower cylinder 2. A flange 31 is provided at the top of the cell sieve 3, and the flange 31 is detachably connected to the upper cylinder 1. Figure 2 As shown, the connection method can be a snap-fit ​​connection in the form of a buckle (flange) and a slot, or an insertion connection in the form of a slot and a insert (flange).

[0026] The collection section is a centrifuge tube 12. The lower cylinder 2 is connected to the centrifuge tube 12 via a converter 13. The converter has an interface that can be matched with syringes and centrifuge tubes 12.

[0027] The grinding section includes a push rod 4 and a rocker arm 5. A piston head 6 is located at the lower end of the push rod 4, and the piston head 6 slides tightly against the upper cylinder 1. A continuous through hole 7 is located at the center of both the push rod 4 and the piston head 6. The rocker arm 5 slides through the through hole 7, with its upper end extending out of the push rod 4 and connecting to a crank handle 8, and its lower end extending out of the piston head 6 and connecting to a grinding pestle 9. The centerline of the grinding pestle 9 is parallel to but does not coincide with the centerline of the rocker arm 5, resulting in a larger grinding coverage area. The maximum distance from the bottom edge of the grinding pestle 9 to the center of the rocker arm 5 is less than the inner diameter of the cell sieve 3. A liquid addition tube 10 is located on the side wall of the upper cylinder 1 for adding the auxiliary liquid required for grinding. One end of the liquid addition tube 10 is located inside the upper cylinder 1, and the other end is located outside the upper cylinder 1. A plug 14 is located at the outer end of the liquid addition tube 10 to prevent dust accumulation. The minimum distance from the center of the rocker arm 5 to the side of the grinding pestle 9 is less than the distance from the center of the rocker arm 5 to the inner end of the liquid adding tube 10. When the grinding pestle 9 moves up and down, the rocker arm 5 can be used to turn the grinding pestle 9 to the side away from the liquid adding tube 10, which can prevent the grinding pestle 9 from colliding with the liquid adding tube 10.

[0028] The method of using this utility model is as follows: Connect the cell sieve 3 containing mouse spleen tissue to the upper cylinder 1, connect the lower cylinder 2 to the centrifuge tube 12, and then connect the upper cylinder 1 to the lower cylinder 2. Add auxiliary liquid to the cell sieve 3 through the liquid addition tube. Then move the rocker arm 5 down to make the grinding pestle 9 fully contact the spleen tissue. Operate the rocker handle 8 to rotate the grinding pestle 9 for grinding. After the cells are released, move the push rod 4 down. The negative pressure causes the cell fluid to be filtered through the double filter and concentrated in the centrifuge tube 12.

[0029] Of course, there may be other embodiments of this utility model. Without departing from the spirit and essence of this utility model, those skilled in the art can make various corresponding changes and modifications based on this utility model, but these corresponding changes and modifications should all fall within the protection scope of the appended claims of this utility model.

Claims

1. A sterile syringe-type spleen lymphocyte separation and extraction device, characterized in that: It includes a separation section, a grinding section, and a collection section. The separation section is syringe-shaped and includes a detachably connected upper cylinder (1) and a lower cylinder (2). The lower end of the upper cylinder (1) is detachably connected to a cell sieve (3), and the lower end of the lower cylinder (2) is detachably connected to the collection section. The grinding section includes a push rod (4) and a rocker arm (5). The lower end of the push rod (4) is provided with a piston head (6). The piston head (6) is in close sliding fit with the upper cylinder (1). The center of the push rod (4) and the piston head (6) is provided with a continuous through hole (7). The rocker arm (5) slides through the through hole (7). The upper end extends out of the push rod (4) and is connected to a rocker arm (8). The lower end extends out of the piston head (6) and is connected to a grinding pestle (9). The side wall of the upper cylinder (1) is provided with a liquid addition tube (10). One end of the liquid addition tube (10) is located inside the upper cylinder (1), and the other end is located outside the upper cylinder (1).

2. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 1, characterized in that: The lower cylinder (2) is equipped with a cell filter (11), the pore size of which is smaller than that of the cell sieve (3).

3. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 2, characterized in that: The outer diameter of the cell sieve (3) is smaller than the inner diameter of the upper cylinder (1) and the lower cylinder (2). The top of the cell sieve (3) is provided with a flange (31), which is detachably connected to the upper cylinder (1).

4. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 3, characterized in that: The cell sieve (3) is snapped or inserted into the upper cylinder (1).

5. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 1, characterized in that: The center line of the grinding pestle (9) is parallel to but does not coincide with the center line of the rocker arm (5).

6. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 5, characterized in that: The minimum distance from the center of the rocker arm (5) to the side of the grinding pestle (9) is less than the distance from the center of the rocker arm (5) to the inner end of the liquid adding tube (10).

7. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 5, characterized in that: The maximum distance from the bottom edge of the grinding pestle (9) to the center of the rocker arm (5) is less than the inner diameter of the cell sieve (3).

8. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 1, characterized in that: The upper cylinder (1) and the lower cylinder (2) are threaded together.

9. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 1, characterized in that: The collection section is a centrifuge tube (12), and the lower cylinder (2) is connected to the centrifuge tube (12) via a converter (13).

10. The sterile syringe-type spleen lymphocyte separation and extraction device according to claim 1, characterized in that: The liquid addition tube (10) is provided with a plug (14) at its outer end.

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