Temperature gradient controlled flask rack for actinomyces fermentation

Abstract

The utility model belongs to the field of actinomycete fermentation technology, and disclose the temperature gradient control flask rack for actinomycete fermentation, including flask machine body, be provided with the heat preservation casing on flask machine body, the front side hinged of heat preservation casing has the observation window, the inner chamber of heat preservation casing is provided with the rack, the top of rack is evenly provided with flask positioning seat, the interval of flask positioning seat is provided with the heat insulating plate, be provided with bottle body positioning mechanism on the rack, bottle body positioning mechanism includes adjusting assembly, positioning clamping plate and protection pad, the top of heat preservation casing is provided with the partition heating assembly, through the partition heating of heat insulating plate cooperation partition heating assembly, can test the fermentation effect of actinomycete under different temperature simultaneously, and except temperature, the oscillation rate, the liquid loading, the culture time and so on condition are completely identical when testing, solve the problem of traditional equipment needing multiple tests, inaccurate data.

Description

Technical Field

[0001] This invention belongs to the field of actinomycete fermentation technology, specifically a temperature gradient control shaker for actinomycete fermentation. Background Technology

[0002] In actinomycete fermentation experiments, traditional shaker racks are mostly designed for single temperature zones, which cannot simultaneously provide multiple independent and stable temperature environments to test the fermentation effect of actinomycetes at different temperatures. Multiple experiments are required for verification. However, during these multiple experiments, key conditions such as oscillation rate, liquid volume, and incubation time are easily affected by changes in environmental temperature and humidity, as well as differences in operation, leading to deviations. This results in low accuracy and poor repeatability of experimental data, and significantly prolongs the experimental cycle. At the same time, traditional shaker fixing structures are mostly fixed-size brackets or simple clamps, which cannot accurately adjust the clamping force according to the diameter of the shaker, resulting in poor adaptability. During oscillation, either insufficient clamping force causes the shaker to tip over and leak, or excessive clamping force causes the shaker to break. It is difficult to achieve efficient and stable positioning of the shaker, which seriously affects the smooth conduct of actinomycete fermentation experiments and the reliability of data. It cannot meet the laboratory's precise needs for screening actinomycete strains and optimizing fermentation conditions.

[0003] Therefore, a temperature gradient control shaker for actinomycete fermentation is proposed to address the above problems. Utility Model Content

[0004] To address the problems mentioned in the background art, this utility model provides a temperature gradient control shaker for actinomycete fermentation. It features simultaneous testing of actinomycete fermentation under multiple temperature conditions through a heat insulation plate and zoned heating components, ensuring complete consistency in experimental conditions such as oscillation rate and liquid volume, except for temperature. This solves the problems of traditional equipment requiring multiple tests and inaccurate data. Furthermore, by using the adjusting components of the bottle positioning mechanism to link the positioning clamp and protective pad, precise clamping of the shaker is achieved, preventing tipping, leakage, and breakage during oscillation. This facilitates efficient and stable shaker positioning.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a temperature gradient control shaker for actinomycete fermentation, comprising a shaker body, an insulated shell on the shaker body, an observation window hinged to the front side of the insulated shell, a placement rack in the inner cavity of the insulated shell, shaker positioning seats evenly arranged on the top of the placement rack, heat insulation plates arranged at intervals of the shaker positioning seats, a bottle positioning mechanism on the placement rack, the bottle positioning mechanism including an adjustment component, a positioning clamp and a protective pad, and a zoned heating component on the top of the insulated shell.

[0006] Preferably, a guide frame is fixed on the inner wall of the insulation shell, and the two ends of the placement frame are slidably connected inside the guide frame.

[0007] Preferably, a limiting screw is provided at one end of the guide frame near the opening, and a limiting screw hole matching the limiting screw is provided on the guide frame.

[0008] Preferably, the adjustment assembly includes a rotating handle, an adjusting screw, and a moving block. The adjusting screw is disposed inside the mounting frame, one end of the adjusting screw is fixed to the rotating handle, and the moving block is threadedly connected to the adjusting screw.

[0009] Preferably, the positioning clamps are two plates extending along the length of the placement rack, and are symmetrically arranged on both sides above the corresponding shaker positioning seat arrangement area.

[0010] Preferably, the placement rack is U-shaped, and a guide groove is provided on the placement rack at the position corresponding to the moving block. One end of the positioning clamp passes through the guide groove and is fixedly connected to the moving block.

[0011] Preferably, the positioning clamp has a groove that matches the shape of the outer wall of the shaker, and the protective pad is attached to the inner wall of the groove.

[0012] Preferably, the inner walls on both sides of the placement rack are provided with slots that match the heat insulation plate, the heat insulation plate is slidably connected in the slots, and the heat insulation plate is disposed between adjacent temperature zones of the zone heating assembly.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] 1. This utility model, through the combination of a heat insulation plate and a zoned heating component, can simultaneously test the fermentation effect of actinomycetes at different temperatures. Moreover, during the test, except for the temperature, the oscillation rate, liquid volume, culture time and other conditions are completely consistent, solving the problem of traditional equipment requiring multiple tests and inaccurate data.

[0015] 2. This utility model uses a bottle positioning mechanism and a rotating adjustment component to precisely clamp the shaker bottle with a positioning clamping plate. This not only prevents the shaker bottle from tipping over and leaking during shaking, but also uses a protective pad to buffer the clamping force, preventing damage to the shaker bottle and facilitating the clamping and positioning of the shaker bottle. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of the thermal insulation shell of this utility model;

[0018] Figure 3 This is a schematic diagram of the placement rack structure of this utility model;

[0019] Figure 4 This is a schematic diagram of the bottle positioning mechanism of this utility model;

[0020] Figure 5 This is a schematic diagram of the adjustment component structure of this utility model.

[0021] In the diagram: 1. Shaker body; 2. Insulated shell; 3. Observation window; 4. Placement rack; 5. Shaker positioning seat; 6. Heat insulation plate;

[0022] 7. Bottle positioning mechanism;

[0023] 71. Adjustment component; 711. Rotary handle; 712. Adjustment screw; 713. Moving block;

[0024] 72. Positioning clamp; 73. Protective pad;

[0025] 8. Zoned heating assembly; 9. Guide frame; 10. Limiting screw. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] The following describes an embodiment of this utility model based on its overall structure.

[0028] like Figures 1 to 5 As shown, this utility model provides a temperature gradient control shaker rack for actinomycete fermentation, including a shaker body 1, an insulated shell 2 on the shaker body 1 to form a closed temperature control space, an observation window 3 hinged to the front of the insulated shell 2, the observation window 3 being connected to the insulated shell 2 via a hinge, a placement rack 4 being provided in the inner cavity of the insulated shell 2, horizontally placed in the inner cavity of the insulated shell 2, shaker positioning seats 5 being evenly arranged on the top of the placement rack 4 to initially fix the shaker, heat insulation plates 6 being provided at intervals of the shaker positioning seats 5, the heat insulation plates 6 being ceramic fiber heat insulation plates, a bottle positioning mechanism 7 being provided on the placement rack 4, the bottle positioning mechanism 7 including an adjustment component 71, a positioning clamp 72 and a protective pad 73, a zoned heating component 8 being provided on the top of the insulated shell 2, consisting of multiple sets of independent silicone heating pads and temperature sensors, each set of heating pads and sensors corresponding to a shaker positioning seat 5 area, forming an independent temperature zone.

[0029] In this embodiment, a guide frame 9 is fixed on the inner wall of the insulation shell 2, and the two ends of the placement frame 4 are slidably connected in the guide frame 9, so that the placement frame 4 can be pulled out of the insulation shell 2 as a whole.

[0030] A limiting screw 10 is provided at one end of the guide frame 9 near the opening. The guide frame 9 is provided with a limiting screw hole that matches the limiting screw 10. When the placement frame 4 is pushed to the preset position along the guide frame 9, the limiting screw 10 is tightened clockwise so that its end is screwed into the placement frame 4, thereby fixing the placement frame 4.

[0031] The adjustment assembly 71 includes a rotating handle 711, an adjusting screw 712, and a moving block 713. The adjusting screw 712 is disposed inside the placement frame 4, and one end of the adjusting screw 712 is fixed to the rotating handle 711. The moving block 713 is threadedly connected to the adjusting screw 712. The positioning clamp 72 consists of two plates extending along the length of the placement frame 4 and symmetrically disposed on both sides above the arrangement area of ​​the corresponding shaker positioning seat 5. Rotating the rotating handle 711 of the adjustment assembly 71 causes the adjusting screw 712 to rotate, causing the moving block 713 to move along the adjusting screw 712, thereby driving the plates of the positioning clamp 72 to move relative to each other.

[0032] The placement rack 4 is arranged in the shape of a c, with the opening facing upwards, which facilitates the placement of the shaker. A guide groove is provided on the placement rack 4 at the position corresponding to the moving block 713. One end of the positioning clamp 72 passes through the guide groove and is fixedly connected to the moving block 713. The guide groove restricts the movement direction of the positioning clamp 72.

[0033] The positioning clamp 72 has a clamping groove that matches the shape of the outer wall of the shaker. The protective pad 73 is attached to the inner wall of the clamping groove, and the silicone protective pad can buffer the clamping force.

[0034] The inner walls on both sides of the placement rack 4 are provided with slots that match the heat insulation plate 6. The heat insulation plate 6 is slidably connected in the slots. The heat insulation plate 6 is set between adjacent temperature zones of the zone heating assembly 8. The heat insulation plate 6 is inserted into the slot from the top of the placement rack 4 so that it stands vertically between adjacent shaker positioning seats 5, reducing heat leakage between temperature zones.

[0035] The shaker body 1 is existing technology and will not be described in detail here. In addition, the present invention also includes a power supply, a controller and a switch, etc., which are not the main technical points and will not be described in detail here.

[0036] The working principle and process of the temperature gradient control shaker rack for actinomycete fermentation: The shaker positioning seat 5 at the top of the rack 4 is used to support the shaker. The heat insulation plate 6 between adjacent shaker positioning seats 5 slides into the slot, dividing the rack 4 into multiple independent areas. Together with the zone heating component 8 at the top, they form physically isolated temperature zones. The zone heating component controls the temperature of each temperature zone individually. The heat insulation plate 6 blocks heat transfer between temperature zones, ultimately building a stable temperature gradient. The shaker is fixed by the bottle positioning mechanism 7. Rotating the rotation handle 711 of the adjustment component 71 drives the adjustment screw 712 to rotate, causing the moving block 713 to move along the adjustment screw 712. The movement drives the positioning clamps 72 on both sides to open and close through the guide grooves; the arc-shaped clamping grooves of the positioning clamps 72 fit the outer wall of the shake flask, and the inner wall protective pad 73 buffers the clamping force, so as to achieve stable fixation of shake flasks of different specifications and avoid shaking and leakage during oscillation. During the fermentation process, the shake flask body 1 oscillates the placement rack 4 and the shake flask, and the zone heating component 8 maintains the temperature of each temperature zone in real time. The placement rack 4 is stably supported under the limit fixation, and the bottle positioning mechanism 7 ensures the safety of the shake flask. Finally, it provides a fermentation environment with multiple temperature gradients for the actinomycetes, meets the needs of strain screening and condition optimization, and solves the problems of traditional equipment requiring multiple tests and inaccurate data.

[0037] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A temperature gradient controlled shaker for actinomycete fermentation, comprising a shaker body (1), characterized in that: The shaker body (1) is provided with a heat-insulating shell (2), and an observation window (3) is hinged to the front side of the heat-insulating shell (2). The inner cavity of the heat-insulating shell is provided with a placement rack (4). Shake positioning seats (5) are evenly arranged on the top of the placement rack (4). Heat insulation plates (6) are arranged at the intervals of the shake positioning seats (5). The placement rack (4) is provided with a bottle positioning mechanism (7). The bottle positioning mechanism (7) includes an adjustment component (71), a positioning clamp (72), and a protective pad (73). The top of the heat-insulating shell (2) is provided with a zone heating component (8).

2. The temperature gradient control shaker for actinomycete fermentation according to claim 1, characterized in that: A guide frame (9) is fixed on the inner wall of the heat-insulating shell (2), and the two ends of the placement frame (4) are slidably connected inside the guide frame (9).

3. The temperature gradient control shaker for actinomycete fermentation according to claim 2, characterized in that: The guide frame (9) is provided with a limiting screw (10) at one end near the opening, and the guide frame (9) is provided with a limiting screw hole that matches the limiting screw (10).

4. The temperature gradient control shaker for actinomycete fermentation according to claim 3, characterized in that: The adjustment assembly (71) includes a rotating handle (711), an adjusting screw (712), and a moving block (713). The adjusting screw (712) is disposed in the placement frame (4). One end of the adjusting screw (712) is fixed to the rotating handle (711), and the moving block (713) is threadedly connected to the adjusting screw (712).

5. The temperature gradient control shaker for actinomycete fermentation according to claim 4, characterized in that: The positioning clamp (72) consists of two plates extending along the length of the placement rack (4) and symmetrically arranged on both sides above the arrangement area of ​​the corresponding shaker positioning seat (5).

6. The temperature gradient control shaker for actinomycete fermentation according to claim 5, characterized in that: The placement rack (4) is arranged in a U-shape. A guide groove is provided on the placement rack (4) at the position corresponding to the moving block (713). One end of the positioning clamp (72) passes through the guide groove and is fixedly connected to the moving block (713).

7. The temperature gradient control shaker for actinomycete fermentation according to claim 6, characterized in that: The positioning clamp (72) has a clamping groove that matches the shape of the outer wall of the shaker, and the protective pad (73) is attached to the inner wall of the clamping groove.

8. The temperature gradient control shaker for actinomycete fermentation according to claim 7, characterized in that: The inner walls on both sides of the placement rack (4) are provided with slots that match the heat insulation plate (6). The heat insulation plate (6) is slidably connected in the slots and is disposed between adjacent temperature zones of the zone heating assembly (8).

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