A 3d cell microsphere or organoid culture well plate
By designing 3D cell microspheres or organoid culture plates, and using matrix microplates to achieve interference-free medium exchange, the problem of 3D cell microspheres being easily moved or washed away during medium exchange in existing technologies is solved, thus improving the stability and accuracy of experiments.
Patent Information
- Application Number
- CN202521577040.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-28
- Publication Date
- 2026-06-26
- Estimated Expiration
- 2035-07-28
AI Technical Summary
In existing technologies, 3D cell microspheres are easily moved or washed away during media exchange, affecting experimental accuracy and increasing operational complexity.
Design a 3D cell microsphere or organoid culture plate, including a culture plate base plate and a cover plate. The cover plate has a matrix-type microplate. Microspheres or tumor organoids are constructed and cultured on the microplate. Interference-free medium exchange is achieved through the micropores. The cover plate has a culture medium filling port at the corner.
This method enables the maintenance of the stability and integrity of 3D cell microspheres during media exchange, simplifies the operation process, and improves the accuracy and efficiency of experiments.
Smart Images
Figure CN224411785U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biomedical engineering technology, and in particular to a 3D cell microsphere or organoid culture plate. Background Technology
[0002] In traditional two-dimensional cell culture, cells are typically grown on a flat substrate or plate. While this method is convenient for manipulation and observation, the cell growth state, morphology, differentiation, and function often differ significantly from their natural state in vivo. To overcome these limitations, scientists have developed three-dimensional cell culture technology, or 3D cell culture. This technology simulates the three-dimensional growth environment of cells in vivo, providing them with a more natural microenvironment, thus allowing cell behavior and responses to more closely resemble their physiological state.
[0003] A key application of 3D cell culture is the construction of three-dimensional cell spheres or organoids, structures that not only morphologically mimic real organs but also exhibit similar functional properties. This method allows researchers to cultivate various types of organoids for studying organ development mechanisms, drug screening, and developing cancer treatment strategies.
[0004] With the continuous advancement of 3D cell culture technology, it has achieved remarkable success in simulating complex tissues and organs, and has shown great potential in fields such as disease modeling, drug development, and toxicology testing. However, for large-sized 3D organoids, maintaining their structural stability and integrity during bioassays remains a pressing technical challenge. Existing techniques, using U-shaped bottoms or low-adhesion cell culture plates for culturing 3D organoids (cell spheres), while able to simulate the in vivo environment to some extent, often result in the organoids (cell spheres) moving or being washed away during medium changes. This not only affects the accuracy of the experiment but also increases the complexity of the operation. Utility Model Content
[0005] The present invention aims to provide a 3D cell microsphere or organoid culture plate to solve the problem that the existing medium change process easily causes the 3D cell microspheres to be moved or washed away.
[0006] To achieve the above objectives, this utility model provides the following technical solution: a 3D cell microsphere or organoid culture plate, comprising a culture plate bottom plate and a culture plate cover, wherein the top of the culture plate bottom plate has an installation groove, the culture plate cover includes a culture plate top plate that cooperates with the installation groove, the culture plate top plate is provided with a plurality of culture grooves, the bottom of the culture grooves is provided with a matrix-type microplate, and the sidewalls of the culture grooves are provided with a plurality of micropores.
[0007] Preferably, the diameter of the micropore is 1 μm.
[0008] Preferably, the number of culture tanks is 6, 12, 48 or 96, and the diameter of the culture tanks is 5 to 40 mm and the depth is 10 to 20 mm.
[0009] Preferably, after the culture well plate cover is installed, there is a distance of 1 to 2 mm between the bottom of the mounting groove and the bottom of the inner wall of the culture well plate base plate.
[0010] Preferably, a culture medium filling and replacement port is provided at one corner of the top plate of the culture plate.
[0011] Preferably, the matrix microplate has a plurality of micropores, and the micropores have multiple sizes, with a height of 0.5 to 2 mm and a diameter of 0.2 to 1 mm.
[0012] The principle and beneficial effects of this technical solution:
[0013] The device is equipped with a culture well plate cover and a culture well plate base plate. Microplates of different sizes are placed in the culture medium in the culture well plate cover plate. Microspheres or tumor organoids can be constructed and cultured on the microplates. Culture medium and cation and cation exchange are carried out through the culture well plate base plate. The solution added in the culture well plate base plate enters the culture tank in the culture well plate cover plate through the micropores, thus realizing interference-free medium exchange.
[0014] The culture well plate cover has a culture medium filling and replacement port at one corner of the top plate, which allows for the removal of the culture well plate cover. At the same time, the culture medium filling and replacement port can also be used as a replacement port for adding and removing culture medium. Attached Figure Description
[0015] Figure 1 A schematic diagram of the structure of a 3D cell microsphere or organoid culture plate provided in an embodiment of this utility model;
[0016] Figure 2 A schematic diagram of a culture tank structure for a 3D cell microsphere or organoid culture well plate provided in an embodiment of this utility model;
[0017] In the diagram: 1. Culture plate bottom plate; 2. Culture plate cover; 3. Mounting groove; 4. Culture plate top plate; 5. Culture tank; 6. Micropores; 7. Culture medium filling and changing port; 8. Matrix microplate. Detailed Implementation
[0018] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments:
[0019] like Figure 1The 3D cell microsphere or organoid culture plate shown includes a culture plate base plate and a culture plate cover plate. The culture plate base plate has an installation groove at its top. The culture plate cover plate includes a culture plate top plate that mates with the installation groove. A culture medium filling and replacement port is provided at one corner of the culture plate top plate, which facilitates the separation of the culture plate cover plate and the culture plate base plate, or serves as a replacement port for adding and removing culture medium.
[0020] The top plate of the culture well plate is provided with several culture tanks, the number of which is 6, 12, 48 or 96, the diameter of the culture tank is 5 to 40 mm and the depth is 10 to 20 mm.
[0021] A matrix-type microplate is installed at the bottom of the culture tank, with conical micropores arranged in a matrix pattern inside the microplate. The matrix-type microplate is a substrate design, containing numerous micropores of various sizes, ranging from 0.5 to 2 mm in height and 0.2 to 1 mm in diameter, to accommodate the preparation of microspheres of different sizes. The related technology for matrix-type microplates is relatively mature; this invention utilizes existing technology that conforms to these dimensions and will not be described in detail.
[0022] The culture tank has several micropores on its sidewalls, each with a diameter of 1 μm. The culture medium can enter and exit through the micropores, while the cell microspheres will not fall from the micropores into the bottom plate of the culture well.
[0023] After the culture well plate cover is installed, there is a 1-2mm distance between the bottom of the installation groove and the bottom of the inner wall of the culture well plate base.
[0024] The specific implementation process is as follows:
[0025] The prepared culture plate caps are sterilized and then placed into the culture plate base to complete the assembly. Cell suspension is prepared and added to the culture tank using a pipette. Culture medium is added to the culture plate base. When changing the culture medium, the culture plate cap is slowly lifted from the culture medium inlet and the liquid in the culture plate base is replaced. This allows for undisturbed culture medium replacement. Alternatively, the culture medium inlet can be used as the culture medium inlet, and culture medium can be added and removed directly from the culture medium inlet using a pipette.
[0026] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions or characteristics are not described in detail here. For those skilled in the art, various modifications and improvements can be made without departing from the technical solution of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.
Claims
1. A 3D cell microsphere or organoid culture plate, characterized in that: The device includes a culture plate base plate and a culture plate cover. The culture plate base plate has an installation groove at its top. The culture plate cover includes a culture plate top plate that mates with the installation groove. The culture plate top plate has a plurality of culture tanks. A matrix-type microplate is provided at the bottom of each culture tank. The sidewalls of the culture tanks have a plurality of micropores.
2. The 3D cell microsphere or organoid culture plate according to claim 1, characterized in that: The diameter of the micropore is 1 μm.
3. The 3D cell microsphere or organoid culture plate according to claim 1, characterized in that: The number of culture tanks is 6, 12, 48 or 96, and the diameter of the culture tanks is 5 to 40 mm and the depth is 10 to 20 mm.
4. A 3D cell microsphere or organoid culture plate according to claim 1, characterized in that: After the culture well plate cover is installed, there is a distance of 1-2 mm between the bottom of the installation groove and the bottom of the inner wall of the culture well plate base.
5. A 3D cell microsphere or organoid culture plate according to claim 1, characterized in that: A culture medium filling and replacement port is located at one corner of the top plate of the culture plate.
6. A 3D cell microsphere or organoid culture plate according to claim 1, characterized in that: The matrix-type microplate has a number of micropores, and the micropores have multiple sizes, ranging from 0.5 to 2 mm in height and 0.2 to 1 mm in diameter.