An enzyme separation device

Abstract

This utility model relates to the field of chemical equipment technology, and provides an enzyme separation device, including a vessel body, a filter cover, a filter plate, a drain pipe, and an outlet pipe. Several filter covers are arranged on the lower part of the inner peripheral wall of the vessel body, and several filter plates are arranged at the bottom of the vessel body. Both the filter plates and filter covers are used to separate porous resin with attached enzyme powder from the solution. Several drain pipes are arranged on the outer peripheral wall of the vessel body, located at the bottom inside the filter covers, and are used to discharge the solution filtered by the filter covers. An outlet pipe is arranged at the bottom of the vessel body to discharge the solution filtered by the filter plates. This device is used to separate the solution that has completed the enzyme-catalyzed reaction from the porous resin with attached enzyme powder. The collected porous resin with attached enzyme powder can be reused, improving the utilization rate of the porous resin with attached enzyme powder. The filter cover and filter plate solve the problems of low solution purity and difficulty in enzyme collection in the prior art.

Description

Technical Field

[0001] This utility model relates to the field of chemical equipment technology, specifically to an enzyme separation device. Background Technology

[0002] Enzymes are widely used as highly efficient biocatalysts in fields such as biocatalysis, food processing, and pharmaceutical preparation. After the reaction, the enzyme needs to be separated and recovered from the reaction system to ensure product purity and achieve enzyme recycling, thereby reducing production costs. Currently, commonly used enzyme separation methods mainly include centrifugation and membrane separation.

[0003] However, existing separation methods have significant drawbacks in practical applications: First, centrifugation relies on parameters such as rotation speed and time. If these parameters are not set appropriately, enzymes may not completely settle and remain in the product solution, severely affecting solution purity. Second, while membrane separation technology offers high separation precision, enzyme proteins are easily adsorbed onto the membrane surface, causing membrane fouling and rapid decline in membrane flux. Furthermore, adsorbed enzymes are difficult to completely desorb and recover through conventional washing, resulting in wasted enzyme resources. In addition, existing separation devices are mostly intermittent in operation, making them unsuitable for the demands of continuous industrial production, further limiting the efficiency and economy of the enzyme separation process. These solutions fail to address the technical problems of low solution purity and difficult enzyme collection, severely hindering the industrial application of enzyme catalysis. Therefore, a highly efficient, stable enzyme separation device suitable for continuous production is urgently needed to overcome these shortcomings. Utility Model Content

[0004] To overcome the above-mentioned defects, this utility model provides an enzyme separation device, which solves the technical problems of low solution purity and difficulty in enzyme collection in the prior art.

[0005] According to one aspect, at least one embodiment of the present invention provides an enzyme separation device for separating a porous resin coated with enzyme powder and a solution, comprising:

[0006] The vessel body;

[0007] The filter cover has a plurality of filter covers, which are disposed below the inner peripheral wall of the vessel and are arranged at intervals along the inner peripheral wall of the vessel.

[0008] A filter plate is disposed inside the vessel and located below the filter cover. Both the filter plate and the filter cover are used to separate porous resin and solution with enzyme powder attached.

[0009] The drain pipe has several drain pipes, each of which is disposed on the outer peripheral wall of the vessel body. Each drain pipe corresponds to a filter cover and is located at the bottom inner side of the filter cover. The drain pipe is used to discharge the solution filtered by the filter cover.

[0010] A liquid outlet pipe is located at the bottom of the vessel body and is used to discharge the solution filtered by the filter plate.

[0011] Optional, also includes:

[0012] The backflush tubes are arranged in a plurality of form, each penetrating the vessel body. The backflush tubes are respectively positioned inside the filter hood and below the filter plate. Each backflush tube has an air inlet and several air outlets. The air inlet is located outside the vessel body, and the air outlets are spaced apart on the tube body of the backflush tube. The air outlets face the filter hood or the filter plate and are used to spray gas onto the filter hood and the filter plate to blow off the porous resin with enzyme powder adhering to them.

[0013] Optionally, the filter plate includes:

[0014] The mounting frames are of several kinds, and the mounting frames are arranged circumferentially in the vessel body. The mounting frames are located on the same horizontal plane, and the side walls of adjacent mounting frames abut against each other.

[0015] The filter screen has several screens, and each screen corresponds to a mounting frame. The screens are detachably mounted on the mounting frame.

[0016] Optionally, the filter screen is further covered with several layers of filter cloth.

[0017] Optionally, the filter cover is rectangular, and at least one side of the outer wall of the filter cover is fitted with a filter screen.

[0018] Optionally, the vessel body further includes:

[0019] The feed pipe has several feed pipes, which are disposed on the top surface of the vessel body. The feed pipes are used to add porous resin and solution with enzyme powder attached to them into the vessel body.

[0020] Optionally, the vessel body includes:

[0021] cylindrical body;

[0022] A base is detachably mounted on the bottom of the cylinder, and a sealing ring is provided between the bottom surface of the cylinder and the top surface of the base.

[0023] Optionally, the longitudinal section of the base is an isosceles trapezoid, and the liquid outlet pipe is located in the middle of the base.

[0024] Optional, also includes:

[0025] An electric motor is disposed on the outer side of the top of the cylinder.

[0026] A stirring rod, one end of which is located on the power output end of the motor, and the other end of which penetrates the top wall of the cylinder and extends into the cylinder body.

[0027] Optionally, a movable frame is provided on the outer side of the bottom of the base.

[0028] The beneficial effects of the embodiments of this utility model are as follows:

[0029] In this invention, several filter covers are arranged below the inner peripheral wall of the vessel, and several filter plates are arranged at the bottom of the vessel. Both the filter plates and filter covers are used to separate porous resin with attached enzyme powder from the solution. The device also includes several drain pipes and outlet pipes. The drain pipes are all located on the outer peripheral wall of the vessel, corresponding one-to-one with the filter covers. The drain pipes are located at the bottom inner side of the filter covers and are used to discharge the solution filtered by the filter covers. The outlet pipes are located at the bottom of the vessel and are used to discharge the solution filtered by the filter plates. This device is used to separate the solution that has completed the enzyme catalytic reaction from the porous resin with attached enzyme powder. The collected porous resin with attached enzyme powder can be reused, improving the utilization rate of the porous resin with attached enzyme powder. The filter covers and filter plates solve the problems of low solution purity and difficulty in enzyme collection in the prior art. Attached Figure Description

[0030] To more clearly illustrate the technical solutions in the embodiments of this utility model, the accompanying drawings used in the description of the embodiments of this utility model will be briefly introduced below. Obviously, the drawings described below are merely some exemplary embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the content of the exemplary embodiments of this utility model and these drawings without any creative effort.

[0031] Figure 1 This is a schematic diagram of the enzyme separation device in one embodiment of the present invention;

[0032] Figure 2 for Figure 1 A schematic diagram of the internal structure of the enzyme separation device in the embodiment;

[0033] Figure 3 for Figure 1 Top view of the enzyme separation device in the embodiment;

[0034] Figure 4 for Figure 1 The embodiment shows a schematic diagram of the filter plate structure.

[0035] In the diagram: 1. Kettle body; 101. Feed pipe; 11. Cylinder; 12. Base; 2. Filter cover; 3. Filter plate; 31. Mounting frame; 32. Filter screen; 4. Drain pipe; 5. Outlet pipe; 6. Backflush pipe; 601. Air inlet; 602. Air outlet; 7. Motor; 8. Stirring rod; 9. Moving frame. Detailed Implementation

[0036] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit its scope.

[0037] To keep the drawings concise, only the parts relevant to the utility model are shown schematically in each drawing; these do not represent the actual structure of the product. Furthermore, for ease of understanding, in some drawings, only one of the components with the same structure or function is schematically shown, or only one is labeled. In this document, "a" not only means "only one," but can also mean "more than one," and "several" includes "two" and "more than two."

[0038] In this document, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0039] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0040] In the description of this embodiment, terms such as "upper," "lower," "left," and "right" are based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of description and simplification of operation, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0041] Furthermore, in the description of this application, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0042] like Figures 1-4 As shown, this invention illustrates an enzyme separation device in one embodiment, used to separate porous resin with attached enzyme powder from a solution. It should be noted that after the porous resin acts as a carrier to immobilize the enzyme powder, the substrate in the solution first diffuses to the resin surface, and then diffuses through the pores to the active site of the enzyme. The enzyme catalyzes the conversion of the substrate into a product by lowering the activation energy of the reaction. The product is then released back into the solution through internal and external diffusion. The entire process is a coupling of mass transfer and enzymatic catalysis. The porous structure provides loading space for the enzyme but also affects the mass transfer efficiency and enzyme stability. However, this device is used to separate the solution after the enzyme-catalyzed reaction from the porous resin still attached with enzyme powder. The collected porous resin still attached with enzyme powder can be reused, improving the utilization rate of the porous resin with attached enzyme powder.

[0043] For example, such as Figure 1 As shown, specifically, the enzyme separation device includes a vessel body 1, a filter cover 2, a filter plate 3, a drain pipe 4, and an outlet pipe 5. The vessel body 1 is cylindrical and is used to hold the solution and the porous resin with attached enzyme powder, providing space for the reaction between the solution and the porous resin with attached enzyme powder. Several filter covers 2 are arranged at intervals along the inner circumferential wall of the vessel body 1. The filter plate 3 is located inside the vessel body 1 and below the filter covers 2. Both the filter plate 3 and the filter covers 2 are used to separate the porous resin still with attached enzyme powder and the solution that has completed the enzyme catalytic reaction.

[0044] Several drain pipes 4 are installed on the outer peripheral wall of the vessel body 1, each corresponding to a filter cover 2. The drain pipes 4 are located at the bottom inner side of the filter cover 2 and are used to discharge the solution filtered by the filter cover 2. An outlet pipe 5 is installed at the center of the bottom of the vessel body 1 to discharge the solution filtered by the filter plate 3. The drain pipes 4 and 5 together discharge the solution after the catalytic reaction until the liquid level in the vessel body 1 is lower than the drain pipes 4. The remaining solution is then discharged through the outlet pipe 5, avoiding the problem of incomplete drainage of the solution in the vessel body 1 and minimizing solution loss. The two-stage filtration structure of the filter cover 2 and filter plate 3 significantly reduces enzyme powder residue and solves the problem of insufficient solution purity caused by separation. The staged drainage design enables continuous filtration and drainage, overcoming the limitations of intermittent operation and improving the overall efficiency of the device.

[0045] It should be noted that in some examples, after the solution and the porous resin with attached enzyme powder enter the vessel 1, the enzyme powder attached to the porous resin begins to catalyze the solution. After catalysis, the solution can flow out from the filter cover 2 and filter plate 3 through the drain pipe 4 on the side wall and the outlet pipe 5 at the bottom. The porous resin with attached enzyme powder cannot pass through the filter cover 2 and filter plate 3, so it remains on the filter cover 2 and filter plate 3, making it easy to collect the porous resin for secondary use.

[0046] For example, such as Figure 2 As shown, the enzyme separation device also includes several backflush pipes 6, all of which are installed through the vessel body 1. Specifically, the backflush pipes 6 are L-shaped, and the several backflush pipes 6 are respectively located inside the filter cover 2 and below the filter plate 3. Since the porous resin with enzyme powder attached will stick to the filter cover 2 and the filter plate 3 during the filtration process, resulting in a decrease in the solution outflow rate, each backflush pipe 6 has an air inlet 601 and several air outlets 602. The air inlet 601 is located on the outside of the vessel body 1, and the several air outlets 602 are located on the outside of the vessel body 1. 02 are evenly spaced on the body of the backflush pipe 6, with the air outlet 602 facing the filter cover 2 or filter plate 3. Several air outlets 602 are used to uniformly spray gas onto the filter cover 2 and filter plate 3 to blow off the porous resin with enzyme powder adhering to the filter cover 2 and filter plate 3, so that the porous resin with enzyme powder adhering to it returns to the vessel body 1. This avoids the problem that the porous resin with enzyme powder adhering to it will stick to the filter cover 2 and filter plate 3, causing the solution to not flow out of the vessel body 1, thus affecting the working efficiency of the device.

[0047] It should be noted that when the operator observes a decrease in the liquid discharged from drain pipe 4 and outlet pipe 5, the backflush pipe 6 is opened. The enzyme powder adhering to the filter cover 2 and filter plate 3 is blown back into the reactor body 1 through the backflush pipe 6 to continue catalyzing the liquid reaction. When an increase in the discharged liquid is detected, the backflush pipe 6 is closed, and the device continues to operate. A control valve is installed at the air inlet 601 of the backflush pipe 6. Because a small amount of liquid flows into the backflush pipe 6 when it is discharged from the filter cover 2 and filter plate 3, the control valve needs to be opened when the backflush pipe 6 starts operating to blow out the liquid remaining in the backflush pipe 6, thereby reducing the impact on the normal operation of the backflush pipe 6.

[0048] For example, such as Figure 4 As shown, the filter plate 3 is fan-shaped. Specifically, the filter plate 3 includes a mounting frame 31 and a filter screen 32. Several mounting frames 31 are arranged circumferentially inside the vessel body 1 and are located on the same horizontal plane. The side walls of adjacent mounting frames 31 abut against each other, and the outer periphery of the mounting frames 31 abuts against the inner wall of the vessel body 1. Several mounting through holes are provided on the mounting frames 31, which are spaced apart. The mounting frames 31 can be installed or removed by fasteners, making it easy to replace or repair them. Each mounting frame 31 is provided with a filter screen 32, which is detachably mounted on the mounting frame 31 and connected to the inner wall of the mounting frame 31. It can be removed and replaced by means of a sliding groove or a snap fastener.

[0049] It should be noted that at least three layers of filter cloth are sequentially covered on the filter screen 32. These filter cloths have several pores of different sizes, and the pores are arranged from largest to smallest on the filter screen 32. Installing multiple layers of filter cloth on the filter screen 32 is to prevent the enzyme powder from flowing out of the vessel 1 with the solution, avoiding the problem of low solution purity, and thus improving the yield of the solution.

[0050] For example, such as Figure 1 As shown, in some examples, the cross-section of the filter cover 2 is rectangular or square, and at least one side of the outer wall of the filter cover 2 is equipped with multiple layers of filter screens. The material of the filter screens is not limited here. This also prevents the enzyme powder from flowing out of the vessel body 1, which makes it easier to collect more enzyme powder for reuse and also improves the purity of the solution.

[0051] For example, such as Figure 3 As shown, in some examples, the vessel body 1 is provided with a feed pipe 101, and there are at least two feed pipes 101. These feed pipes 101 are all provided on the top surface of the vessel body 1. The feed pipes 101 are used to add porous resin and solution with enzyme powder attached into the vessel body 1.

[0052] It should be noted that an observation hole is also provided on the top surface of the vessel body 1. The observation hole is used to allow operators to observe the catalytic reaction between the enzyme powder and the solution inside the vessel body 1. It is also used by operators to rinse the inner wall of the vessel body 1 through an external water pipe after all the solution has flowed out of the vessel body 1, to prevent enzyme powder from adhering to the inner wall of the vessel body 1. This is conducive to the collection of enzyme powder. As a biocatalyst, enzymes are usually expensive to prepare. The ability to efficiently recover the enzyme after the reaction allows it to be reused, which greatly reduces enzyme consumption and lowers the raw material cost in production.

[0053] The vessel body 1 includes a cylindrical body 11 and a base 12. The base 12 is detachably mounted on the bottom of the cylindrical body 11. Specifically, the base 12 and the cylindrical body 11 are locked together by bolts and nuts. A sealing ring is provided between the bottom surface of the cylindrical body 11 and the top surface of the base 12 to seal the vessel body 1 when locked, preventing the solution inside the vessel body 1 from flowing out through the connection and reducing solution loss. After the solution is drained, the base 12 can be removed, allowing operators to collect the porous resin for reuse of the enzyme powder in the porous resin to catalyze reactions in other solutions. In addition, after the base 12 is separated from the cylindrical body 11, operators can easily remove the filter cover 2 and the filter plate 3, facilitating cleaning of the vessel body 1, the filter cover 2, and the filter plate 3.

[0054] For example, such as Figure 1 As shown, in some examples, the longitudinal section of the base 12 is an isosceles trapezoid, and the liquid outlet pipe 5 is located in the middle of the base 12 so that the solution can be completely discharged from the base 12, further reducing the solution loss rate.

[0055] For example, such as Figure 1 As shown, in some examples, the enzyme separation device also includes a motor 7 and a stirring rod 8. The motor 7 is located on the outer side of the top of the cylinder 11 and is used to drive the stirring rod 8 to rotate. One end of the stirring rod 8 is located on the power output end of the motor 7, and the other end of the stirring rod 8 passes through the top wall of the cylinder 11 and extends into the cylinder 11. Several stirring paddles are provided on the stirring rod 8 and are spaced apart along the axial direction of the stirring rod 8. The stirring paddles are used to stir the solution in the vessel 1 and the porous resin with enzyme powder attached, so that the solution and the porous resin with enzyme powder attached can be in full contact.

[0056] For example, such as Figure 1 As shown, a movable frame 9 is provided on the outer side of the bottom of the base 12. The movable frame 9 is connected to casters to facilitate the movement of the vessel body 1.

[0057] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. An enzyme separation device for separating a porous resin having enzyme powder adhered thereto from a solution, characterized by comprising: a porous resin having enzyme powder adhered thereto; a solution; and a magnetic field generator for generating a magnetic field, wherein the porous resin and the solution are separated by the magnetic field generated by the magnetic field generator. include: The vessel body (1); A filter cover (2) is provided, and the filter cover (2) is provided in a plurality of them. The plurality of filter covers (2) are disposed below the inner peripheral wall of the vessel body (1), and the plurality of filter covers (2) are arranged at intervals along the inner peripheral wall of the vessel body (1). The filter plate (3) is disposed inside the vessel body (1) and located below the filter cover (2). Both the filter plate (3) and the filter cover (2) are used to separate the porous resin and solution with enzyme powder attached. The drain pipe (4) has several drain pipes, and all drain pipes (4) are arranged on the outer peripheral wall of the vessel body (1). The drain pipe (4) corresponds one-to-one with the filter cover (2). The drain pipe (4) is located at the bottom inside the filter cover (2). The drain pipe (4) is used to discharge the solution filtered by the filter cover (2). The liquid outlet pipe (5) is located at the bottom of the vessel body (1) and is used to discharge the solution filtered by the filter plate (3).

2. The enzyme separation device according to claim 1, characterized in that, Also includes: A backflush pipe (6) is provided, and the backflush pipe (6) is provided through the vessel body (1). The backflush pipe (6) is provided inside the filter cover (2) and below the filter plate (3). Each backflush pipe (6) has an air inlet (601) and a number of air outlets (602). The air inlet (601) is located outside the vessel body (1). The air outlets (602) are provided at intervals on the pipe body of the backflush pipe (6). The air outlets (602) face the filter cover (2) or the filter plate (3). The air outlets (602) are used to spray gas onto the filter cover (2) and the filter plate (3) to blow off the porous resin with enzyme powder adhering to the filter cover (2) and the filter plate (3).

3. The enzyme separation device according to claim 2, characterized in that, The filter plate (3) includes: Mounting frames (31) are provided in a plurality of them. The plurality of mounting frames (31) are arranged circumferentially within the vessel body (1) and are located on the same horizontal plane. The side walls of adjacent mounting frames (31) abut against each other. A filter screen (32) is provided, and there are several filter screens (32) and the mounting frame (31) correspond one-to-one. The filter screen (32) is detachably mounted on the mounting frame (31).

4. The enzyme separation device according to claim 3, characterized in that, The filter screen (32) is also covered with several layers of filter cloth in sequence.

5. The enzyme separation device according to claim 1, characterized in that, The filter cover (2) is rectangular, and at least one side of the outer wall of the filter cover (2) is equipped with a filter screen.

6. The enzyme separation device according to claim 1, characterized in that, The vessel body (1) also includes: Feed pipe (101), there are several feed pipes (101), several feed pipes (101) are arranged on the top surface of the vessel body (1), the feed pipes (101) are used to add porous resin and solution with enzyme powder attached into the vessel body (1).

7. The enzyme separation device according to claim 1, characterized in that, The vessel body (1) includes: Cylinder (11); The base (12) is detachably disposed at the bottom of the cylinder (11), and a sealing ring is provided between the bottom surface of the cylinder (11) and the top surface of the base (12).

8. The enzyme separation device according to claim 7, characterized in that, The longitudinal section of the base (12) is an isosceles trapezoid, and the liquid outlet pipe (5) is located in the middle of the base (12).

9. An enzyme separation device according to claim 8, characterized in that, Also includes: Motor (7), the motor (7) is located on the outer side of the top of the cylinder (11); A stirring rod (8) is provided at one end of the power output end of the motor (7), and the other end of the stirring rod (8) passes through the top wall of the cylinder (11) and extends into the cylinder (11).

10. An enzyme separation device according to claim 8, characterized in that, A movable frame (9) is provided on the outer side of the bottom of the base (12).

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