Tissue cutting device

Abstract

This application discloses a tissue cutting device provided by this utility model, including a housing with a through hole for tissue passage, and a cutting mesh for cutting tissue installed in the through hole. This utility model, through customized material selection and a selectable 0.5–5 mm aperture, precisely adapts to different tissues, significantly reducing mechanical stress damage to fragile cells while avoiding the uneven size problems of traditional manual cutting. The use of different material combinations can specifically address the cutting resistance of high-fiber tissues, the adhesion residue of lipid tissues, and the clogging risk of sticky tissues, improving enzymatic hydrolysis efficiency and cell survival rate.

Description

Technical Field

[0001] This utility model belongs to the field of laboratory auxiliary device technology, and particularly relates to the field of auxiliary devices for tissue and cell pretreatment, specifically a tissue cutting device. Background Technology

[0002] In cell biology and medical research, tissue fragmentation is a crucial step in many experiments. For example, in primary cell culture, when isolating primary cells from tissue, fragmentation increases the contact area between the tissue and digestive enzymes, promoting cell dissociation. Furthermore, when studying proteins, nucleic acids, or metabolites, fragmentation followed by homogenization is more efficient and reduces component loss. When assessing drug permeability and metabolic activity, small tissue fragments can mimic the in vivo microenvironment, improving experimental consistency. When constructing biological scaffolds or organoids, it is necessary to retain a certain tissue structure; fragmentation facilitates subsequent recombination. Large tissue fragments are prone to hypoxia and necrosis; fragmentation improves nutrient / oxygen exchange, increasing survival rates. In summary, tissue fragmentation is an important pretreatment step in many cell research studies.

[0003] Currently, the cutting of ex vivo tissues in biomedical experiments largely relies on manual operations, such as scalpels or scissors, or general mechanical cutting equipment. These methods have significant limitations: manual cutting is inefficient, results in uneven tissue block sizes, leading to inconsistent enzymatic digestion or culture results, and is susceptible to human error due to operator skill differences. While traditional mechanical cutting equipment improves efficiency, the shearing process can easily damage cell viability due to frictional heat or mechanical stress, especially in fragile tissues such as brain tissue or primary tumor cells, significantly impacting survival rates. Furthermore, existing cutting tools have limited material options, lack adaptability to different tissue characteristics, and have complex sterilization processes, making it difficult to balance high-throughput requirements with low contamination risks. These problems severely restrict cell separation efficiency, experimental reproducibility, and clinical translation potential. Based on the above-mentioned problems and practical needs, this utility model was developed. Utility Model Content

[0004] To address the problems of low cutting efficiency, uneven tissue block size, and uneven enzymatic digestion in existing technologies, this application provides a simple and practical tissue cutting device that can perform consistent and efficient cutting for different tissues and different tissue block requirements.

[0005] To achieve the above objectives, the technical solution adopted in this application is as follows:

[0006] The tissue cutting device provided by this utility model includes a housing with a through hole for tissue to pass through, and a cutting mesh for cutting tissue is installed in the through hole.

[0007] To improve the compatibility of this invention for use in multiple scenarios and facilitate the cleaning, maintenance, and replacement of the cutting mesh, preferably, the housing includes an upper housing and a lower housing nested within each other, with the cutting mesh fixedly or detachably fixed to the lower housing or the upper housing; the upper housing has a first through hole, and the lower housing has a second through hole. Making the housing detachable allows for removal and cleaning after each tissue cutting, preventing residual material from corroding the cutting mesh and avoiding contamination for subsequent tissue cutting.

[0008] As another optimized structural configuration, preferably, the housing includes an upper housing and a lower housing nested within each other and each having a first through hole and a second through hole, respectively, and a mesh disposed between the upper and lower housings. The mesh includes a fixing ring embedded in the lower housing and a cutting mesh fixedly disposed on the fixing ring. By making the upper housing, cutting mesh, and lower housing all detachable, it is easier to clean the structure after tissue cutting. Simultaneously, when different tissues need to be cut, the cutting mesh can be easily replaced without needing to replace the upper and lower housings, avoiding housing waste or excessive inventory and improving the effective utilization rate of the housing.

[0009] To facilitate quick and easy installation and fastening, preferably, the inner wall of the lower housing is provided with an inner boss for installing and restricting the mesh, and the outer wall of the upper housing is provided with an outer boss for restricting the relative position between the upper housing and the mesh or the inner boss. The aforementioned inner and outer bosses allow for intuitive installation of the upper and lower housings during the installation process. When the upper and lower housings are nested together and reach the position of the outer boss, it can be clearly determined that they are properly installed without relying on the operator's experience.

[0010] To facilitate tissue cutting and improve cutting efficiency and uniform force distribution, preferably, the upper shell has a bottom plate for sealing the first through hole. When the upper and lower shells are fully nested, the bottom plate is in contact with the cutting mesh. The bottom plate structure provides a different tissue cutting method. Given that the entire tissue cutting device has through holes, the cutting mesh is the only structure that obstructs the tissue. Cutting requires applying a force to the tissue using an operating rod, such as a glass rod or homogenizing rod, in conjunction with the cutting action of the cutting mesh. However, for tissues that are not easily damaged or are very easy to cut, such as brain tissue, the bottom plate of the upper shell can be used to apply uniform pressure to the tissue, and the cutting mesh can then be used for cutting. The operation is also simpler; the upper shell simply moves the tissue toward the cutting mesh, ensuring uniform pressure. Under the combined action of the cutting mesh and the bottom plate's compression, the tissue can be cut evenly, and the proportion of cell damage can be effectively controlled. Of course, the speed at which the upper shell moves and the pressure applied will vary depending on the tissue. To be as gentle as possible, it is preferable to nest the upper and lower shells in a threaded manner. This can effectively control the degree of pressure on the tissue and is suitable for cutting different tissues.

[0011] In order to cut different tissues while reducing other effects of the cutting mesh on the tissue, the cutting mesh is preferably made of any one of stainless steel wire, titanium alloy wire, nylon or Teflon.

[0012] More preferably, the mesh diameter of the cut mesh is 0.5mm-4mm.

[0013] Beneficial effects:

[0014] This invention utilizes cutting meshes with different cutting materials and apertures to cut different tissues, offering the following technical advantages compared to existing technologies:

[0015] 1. This utility model can significantly reduce the damage of mechanical stress to fragile cells by customizing the selection of materials and precisely adapting to different tissues with an optional pore size of 0.5–5 mm, while avoiding the problem of uneven size in traditional manual cutting.

[0016] 2. This utility model uses a combination of different materials to specifically address the cutting resistance of high-fiber tissues, the adhesion residue of lipid tissues, and the clogging risk of sticky tissues, thereby improving enzymatic hydrolysis efficiency and cell survival rate.

[0017] 3. The modular design of the cutting mesh supports rapid replacement and low-temperature sterilization (such as ultraviolet light or ethanol), simplifying the operation process while ensuring sterility, and is especially suitable for high-throughput processing of clinical samples. In addition, the integration of standardized pore size with automated equipment can reduce human error, enhance experimental reproducibility, and provide a more stable tissue pretreatment solution for cutting-edge fields such as organoid construction and precision drug screening, with the advantages of high efficiency, flexibility and low cost. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is the main structural view of this utility model.

[0020] Figure 2 yes Figure 1 A sectional view with the section symbol AA along the center line.

[0021] Figure 3 yes Figure 1 Top view.

[0022] Figure 4 yes Figure 1 Axonometric drawing.

[0023] Figure 5 yes Figure 4 Exploded view.

[0024] In the figure: 1-Upper shell; 11-First through hole; 12-Outer boss; 2-Grid; 21-Cut grid; 3-Lower shell; 31-Inner boss; 32-Second through hole. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0027] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0028] In the description of this application, it should be noted that the use of terms such as "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer" to indicate orientation or positional relationships is based on the orientation or positional relationships shown in the accompanying drawings, or the orientation or positional relationships commonly used when the product is in use. These terms are used solely for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the use of terms such as "first" and "second" in the description of this application is only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0029] Furthermore, the use of terms such as "horizontal" and "vertical" in the description of this application does not imply that the component is required to be absolutely horizontal or suspended, but rather that it may be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but rather that it may be slightly tilted.

[0030] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0031] Example 1:

[0032] Refer to the instruction manual. Figure 1-3As shown, the tissue cutting device provided in this embodiment includes a housing with a through-hole for tissue passage. A cutting mesh 21 for cutting tissue is installed within the through-hole. During operation, the tissue block to be processed is simply placed on the cutting mesh 21, and the tissue is gently moved across the cutting mesh 21 using a sterile abrasive rod. By applying pressure towards the cutting mesh 21, the tissue is shredded. Because the cutting mesh 21 has uniform mesh openings, the tissue cut by the cutting mesh 21 is more uniform and consistent in size compared to tissue blocks cut by existing scissors. Tissue blocks cut by the cutting mesh 21 effectively increase surface area, accelerating enzyme digestion or reagent penetration, such as antibody staining and fixation. It also prevents the death of central cells in large tissue blocks due to hypoxia / accumulation of metabolic waste, and improves the reproducibility of subsequent experiments and allows for the preservation of microstructures such as intercellular connections in specific experiments.

[0033] Example 2:

[0034] To improve the compatibility of this utility model for use in multiple scenarios and to facilitate the cleaning, maintenance, and replacement of the cutting mesh 21, this embodiment optimizes the structure based on Embodiment 1. See the appendix to the specification. Figures 1-3 As shown, the housing includes an upper housing 1 and a lower housing 3 nested within each other. The cutting mesh 21 is fixedly or detachably fixed to the lower housing 3 or the upper housing 1. The upper housing 1 has a first through hole 11, and the lower housing 3 has a second through hole 32. Making the housing detachable allows for easy removal and cleaning after each tissue cutting, preventing residual material from corroding the cutting mesh 21 and avoiding contamination for subsequent tissue cutting. Figure 5 As shown, a technical solution is illustrated where the upper shell 1, the cutting mesh 21, and the lower shell 3 are all detachable structures. When tissue needs to be cut, the upper shell 1, the cutting mesh 21, and the lower shell 3 can be combined into the following configuration: Figure 4 The structure shown is sufficient; it is very convenient and practical.

[0035] Example 3:

[0036] This embodiment provides another optimized structural configuration, which is also a further optimization based on Embodiment 1. See the appendix of the specification for details. Figures 1-5As shown, the housing includes an upper housing 1 and a lower housing 3 nested together and having a first through hole 11 and a second through hole 32 respectively, and a mesh 2 disposed between the upper housing 1 and the lower housing 3. The mesh 2 includes a fixing ring embedded in the lower housing 3 and a cutting mesh 21 fixedly disposed on the fixing ring. The upper housing 1, the cutting mesh 21, and the lower housing 3 are all detachable, which facilitates the cleaning of the tissue after cutting. Simultaneously, when different tissues need to be cut, the cutting mesh 21 can be easily replaced without replacing the upper housing 1 and the lower housing 3, avoiding waste or excessive inventory and improving the effective utilization rate of the housing. In this embodiment, the mesh 2 is a key component of this invention, undertaking the function of cutting tissue. The fixing ring of the mesh 2 mainly functions to: 1. effectively fix the edge of the cutting mesh 21, preventing the mesh wires of the cutting mesh 21 from shifting and scattering, thus fixing the cutting mesh 21. 2. Adaptively matched with the lower housing 3 and the upper housing 1, installed between the lower housing 3 and the upper housing 1, taking into account the effectiveness of cutting and the convenience of disassembly and cleaning.

[0037] To facilitate quick and easy installation and fastening, in this embodiment, the inner wall of the lower housing 3 is provided with an inner boss 31 for installing and restricting the mesh 2. See [link to relevant documentation]. Figure 5 As shown, an outer boss 12 is provided on the outer wall of the upper housing 1 to limit the relative position between the upper housing 1 and the mesh 2 or the inner boss 31. The arrangement of the inner boss 31 and the outer boss 12 allows for intuitive installation of the upper housing 1 and the lower housing 3 during installation. When the upper housing 1 and the lower housing 3 are nested together and reach the position of the outer boss 12, it can be intuitively determined that they are properly installed without relying on the operator's experience.

[0038] Example 4:

[0039] To facilitate tissue cutting, improve cutting efficiency, and enhance the uniformity of force application, such as Figure 5As shown, the upper housing 1 has a bottom plate for sealing the first through hole 11. When the upper housing 1 and the lower housing 3 are in a fully nested state, the bottom plate is in contact with the cutting mesh 21. The bottom plate structure provides a different tissue cutting method. Given that the entire tissue cutting device has through holes, the cutting mesh 21 is the only structure that blocks the tissue. During cutting, a force is applied to the tissue by an operating rod, allowing the cutting mesh 21 to cut the tissue. For example, a glass rod or homogenizing rod can be used to gently cut the tissue, combined with the cutting action of the cutting mesh 21. However, for some tissues that are not easily damaged or are very easy to cut, such as brain tissue, the bottom plate at the bottom of the upper housing 1 can be used to apply uniform pressure to the tissue, and the cutting mesh 21 can be used for cutting. The operation is also simpler; the upper housing 1 is simply moved towards the cutting mesh 21, so that the tissue is evenly compressed. Under the dual action of the cutting mesh 21 and the pressure of the bottom plate, the tissue can be cut evenly, and the proportion of cell damage can be effectively controlled. Of course, the speed at which the upper shell 1 moves and the pressure applied will vary depending on the tissue. To ensure the procedure is as gentle as possible, it is preferable that the upper shell 1 and the lower shell 3 are nested together in a threaded manner. This effectively controls the degree of pressure on the tissue and is suitable for cutting different tissues. When the outer boss 12 contacts the upper surface of the lower shell 3, the upper shell 1 or the base plate will press the cutting mesh 21 against the inner boss 31 and make contact with the cutting mesh 21, squeezing all the tissue to the other side of the cutting mesh 21, thus completing the tissue cutting.

[0040] To target different tissues while minimizing the impact of the cutting mesh 21 on the tissue, in this embodiment, the cutting mesh 21 is made of stainless steel wire, titanium alloy wire, nylon, or Teflon. Furthermore, the mesh diameter of the cutting mesh 21 is 0.5mm-4mm. That is, in practical use, the same upper housing 1 and lower housing 3 can be fitted with multiple cutting meshes 21 of different materials and apertures. Different cutting meshes 21 can be selected as needed for different tissue cutting requirements, making it practical, convenient, efficient, and easy to clean.

[0041] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A tissue cutting device comprising a housing, characterized by: The housing has a through hole for tissue to pass through, and a cutting mesh (21) for cutting tissue is installed in the through hole; the housing includes an upper housing (1) and a lower housing (3) nested together and having a first through hole (11) and a second through hole (32) respectively, and the cutting mesh (21) or grid (2) disposed between the upper housing (1) and the lower housing (3), the grid (2) including a fixing ring embedded in the lower housing (3), and the cutting mesh (21) fixedly disposed on the fixing ring.

2. The tissue cutting device of claim 1, wherein: The inner wall of the lower housing (3) is provided with an inner boss (31) for installing and restricting the grid (2), and the outer wall of the upper housing (1) is provided with an outer boss (12) for restricting the relative position between the upper housing (1) and the grid (2) or the inner boss (31).

3. The tissue cutting device according to claim 1 or 2, characterized in that: The upper housing (1) has a bottom plate for sealing the first through hole (11). When the upper housing (1) and the lower housing (3) are in a fully nested state, the bottom plate is in contact with the cutting mesh (21).

4. The tissue cutting device according to any one of claims 1-2, characterized in that: The upper housing (1) and the lower housing (3) are nested together by a threaded detachable structure.

5. The tissue cutting apparatus according to any one of claims 1-2, characterized in that: The cutting mesh (21) is made of any one of stainless steel wire, titanium alloy wire, nylon or Teflon.

6. The tissue cutting apparatus according to any one of claims 1-2, characterized in that: The mesh diameter of the cut mesh (21) is 0.5mm-4mm.

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