A protein high-temperature oil bath reaction tank system for a laboratory
By using heat transfer oil circulation heating and stirring blade design, combined with a cooling system, the heat transfer simulation problem of high-temperature reaction vessels in the laboratory was solved. This achieved accurate experimental data and safe and efficient heat transfer conditions for high-temperature and high-pressure reaction vessels, meeting the heat transfer requirements of high-temperature and high-pressure reaction vessels in the laboratory and fulfilling experimental requirements.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- YICHUN JINNONG BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-07-21
- Publication Date
- 2026-07-03
AI Technical Summary
The heat transfer conditions of a high-pressure reactor are difficult to simulate under laboratory conditions, resulting in inaccurate experimental data. Furthermore, the static state of the material when using an autoclave affects the interpretation of the data.
It adopts a heat transfer oil circulation heating and a three-layer stirring blade design, combined with the coil embedded design of the cooling system, to achieve dynamic heating and temperature uniformity of the material inside the tank. It uses high-boiling-point heat transfer oil as the heat transfer medium and is equipped with a high-pressure resistant tank.
It accurately simulates the industrial environment, improves the reliability of experimental data, ensures safe and efficient high-temperature and high-pressure reactions, and achieves rapid temperature rise and fall to meet experimental requirements.
Smart Images
Figure CN224443022U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of protein reaction vessel equipment technology, and in particular to a high-temperature oil bath protein reaction vessel system for laboratory use. Background Technology
[0002] Protein production involves high-temperature reactions, and high-pressure reaction vessels are typically used in production, with steam as the medium to achieve the high-temperature process.
[0003] However, during the research and development process, due to limited laboratory conditions, it was inconvenient to use steam as a heat transfer medium to simulate actual production conditions. Typically, an autoclave is used to replace the high-temperature reaction process. However, in an autoclave, the materials are in a static state, and the heat transfer coefficient differs significantly from that of a high-pressure reaction vessel, making it impossible to accurately simulate real-world conditions and severely impacting data analysis during the research and development process. Utility Model Content
[0004] This application addresses the shortcomings of the prior art by providing a high-temperature oil bath reaction vessel system for proteins in the laboratory. The system utilizes circulating heat transfer oil to dynamically heat the materials inside the vessel, ensuring uniform temperature distribution and closely mimicking the heat transfer conditions of high-pressure reaction vessels in industrial production. The cooling system directly cools the heat transfer oil through an embedded coil design, thereby facilitating heat exchange within the reaction vessel. This significantly improves the cooling efficiency of high-temperature materials, meeting experimental requirements.
[0005] The technical solution adopted in this utility model is as follows:
[0006] A high-temperature oil bath reaction vessel system for proteins in a laboratory includes a reaction vessel, the outer wall of which is sequentially provided with a vessel jacket and an insulation layer, and a heating system and a cooling system are respectively provided on the outer sides of the reaction vessel.
[0007] The heating system inputs heat transfer oil from the bottom side of the tank jacket and circulates heat transfer oil from the top side of the tank jacket.
[0008] The cooling system discharges cooling coils from the bottom side of the tank jacket. The cooling coils are arranged around the bottom circumference of the tank jacket to the top. The cooling system inputs cooling water through the cooling coils and circulates cooling water from the top.
[0009] Furthermore, the reaction vessel is equipped with three layers of stirring blades, which are driven by a servo motor located at the top of the vessel. A temperature probe for measuring temperature is also installed inside the reaction vessel.
[0010] Furthermore, a heat transfer oil inlet pipe is provided on the bottom left side of the tank jacket, and a heat transfer oil outlet pipe is provided on the top right side; a cooling water inlet pipe is provided at the bottom of the tank jacket, and a cooling water outlet pipe is provided on the top left side.
[0011] Furthermore, the heating system includes a hot oil tank, in which a heating rod is installed. A heat transfer oil pump and a second solenoid valve are sequentially connected to the bottom discharge end of the hot oil tank, and the liquid outlet end of the heat transfer oil pump is connected to the heat transfer oil inlet pipe.
[0012] Furthermore, a second PLC module and a second temperature sensor are sequentially arranged along the path from the hot oil tank to the second solenoid valve.
[0013] Furthermore, the cooling system includes a cold water tank, the internal circulation of which is equipped with a chiller unit and a unit circulation pump. The bottom outlet of the cold water tank is sequentially connected to a chiller pump and a first solenoid valve, which is connected to the cooling water inlet pipe.
[0014] Furthermore, a first PLC module and a first temperature sensor are sequentially connected along the path from the chiller unit to the chilled water pump.
[0015] The advantages of this utility model over the prior art are as follows:
[0016] Accurately simulates the industrial environment and improves the reliability of R&D data: Through the synergistic effect of heat transfer oil circulation heating and three-layer stirring blades, the material inside the tank is dynamically heated and the temperature distribution is uniform, which highly replicates the heat transfer conditions of high-pressure reaction tanks in industrial production, ensuring the consistency between experimental data and actual production.
[0017] The high-temperature and high-pressure reaction during the experiment is safer: high-boiling-point heat transfer oil is used as the heat transfer medium, avoiding the high risk of using steam in the laboratory; combined with the high-pressure resistant tank design, it meets the requirements of high-temperature and high-pressure processes such as protein disulfide bond destruction, ensuring operational safety.
[0018] Rapid heating and cooling rates and efficient process response: The low heat transfer coefficient of the heat transfer oil, combined with the jacketed direct circulation structure, enables rapid heating of materials; the cooling system directly cools the heat transfer oil through the coil embedded design, which significantly improves the cooling efficiency of high-temperature materials and meets experimental requirements. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the structure of this utility model.
[0020] The components include: 1. Chiller unit; 2. Unit circulating pump; 3. First PLC module; 4. First temperature sensor; 5. Chiller pump; 6. First solenoid valve; 7. Heat transfer oil outlet pipe; 8. Cooling water inlet pipe; 9. Tank jacket; 10. Heat transfer oil inlet pipe; 11. Second solenoid valve; 12. Heat transfer oil pump; 13. Second temperature sensor; 14. Second PLC module; 15. Heating rod; 16. Cooling water outlet pipe; 17. Insulation layer. Detailed Implementation
[0021] The specific embodiments of this utility model are described below with reference to the accompanying drawings.
[0022] This invention provides a high-temperature oil bath reaction vessel system for proteins in the laboratory, aiming to solve the problem that existing technologies use sterilizers to replace high-temperature reaction processes, which cannot accurately simulate the actual heat transfer regulation of the reaction vessel.
[0023] like Figure 1 As shown, the present invention includes a reaction vessel, the outer wall of which is sequentially provided with a vessel jacket 9 and a heat insulation layer 17, and a heating system and a cooling system are respectively provided on the outer sides of the reaction vessel.
[0024] The heating system inputs heat transfer oil from the bottom side of the tank jacket 9 and circulates heat transfer oil from the top side of the tank jacket 9.
[0025] In this invention, heat transfer oil is used as the heat exchange medium. Due to its high boiling point and high thermal stability, it can reach a high temperature of 200℃ under normal pressure, making it a relatively ideal high-temperature heating medium. Compared with steam, it is safer. The heat transfer oil has a low heat transfer coefficient, but this is compensated for by forced circulation and three-layer stirring blades, achieving rapid heating and cooling. During the reaction process, it can quickly meet the process requirements.
[0026] The cooling system discharges cooling coils from the bottom side of the tank jacket 9. The cooling coils are arranged around the bottom circumference of the tank jacket 9 to the top. The cooling system inputs cooling water through the cooling coils and circulates cooling water from the top.
[0027] According to the above structure, the cooling water inlet pipe 8 and the cooling water outlet pipe 16 form a coil structure in the tank jacket 9. The coil structure is embedded in the jacket heat transfer oil. During the cooling process, the cold water cools the heat transfer oil, and the heat transfer oil exchanges heat with the reaction tank wall to further cool the material.
[0028] In one embodiment of this utility model, the reaction vessel is provided with three layers of stirring blades, which are driven by a servo motor located at the top of the vessel. A temperature probe for measuring temperature is also provided inside the reaction vessel.
[0029] In one embodiment of this utility model, a heat transfer oil inlet pipe 10 is provided on the bottom left side of the tank jacket 9, and a heat transfer oil outlet pipe 7 is provided on the top right side; the bottom of the tank jacket 9 has a cooling water inlet pipe 8, and a cooling water outlet pipe 16 is provided on the top left side.
[0030] In one embodiment of this utility model, the heating system includes a hot oil tank, a heating rod 15 is provided in the hot oil tank, and a heat transfer oil pump 12 and a second solenoid valve 11 are connected in sequence to the bottom discharge end of the hot oil tank. The liquid outlet end of the heat transfer oil pump 12 is connected to the heat transfer oil inlet pipe 10.
[0031] In one embodiment of this utility model, a second PLC module 14 and a second temperature sensor 13 are also arranged sequentially along the path from the hot oil tank to the second solenoid valve 11.
[0032] Based on the above structure, the temperature of the heat transfer oil can be controlled by the heating rod 15 through the second PLC module 14 to achieve the required temperature setting. The heated heat transfer oil enters the tank jacket 9 through the pump and the second solenoid valve 11. The higher the temperature of the heat transfer oil, the faster the temperature of the heat exchange material in the jacket will rise, provided that the frequency of the heat transfer oil pump 12 remains constant.
[0033] In one embodiment of this utility model, the cooling system includes a cold water tank, and the cold water tank is equipped with a chiller unit 1 and a unit circulation pump 2 for internal circulation. The bottom outlet of the cold water tank is connected in sequence to a chiller pump 5 and a first solenoid valve 6, and the first solenoid valve 6 is connected to a cooling water inlet pipe 8.
[0034] In one embodiment of this utility model, a first PLC module 3 and a first temperature sensor 4 are sequentially connected along the path from the cooling unit to the chilled water pump 5.
[0035] Based on the above structure, the chilled water temperature can be controlled by the first PLC module 3 to achieve the required temperature setting for the chiller unit 1. The chilled water enters the cooling coil through the pump and the first solenoid valve 6. The lower the chilled water temperature, the faster the heat transfer oil is cooled, provided that the frequency of the chilled water pump 5 remains constant.
[0036] Because the high-temperature heat transfer oil can make the temperature of the material inside the tank >100℃, the material inside the tank is under high pressure during high-temperature reaction. The reaction tank and seals of this structure need to be able to withstand high pressure. Under normal circumstances, a high pressure resistance of 0.3MPa is sufficient to meet the process requirements for protein disulfide bond destruction.
[0037] Working principle:
[0038] The system mainly consists of a cooling water system, a heating system, and a reaction tank. The reaction tank is equipped with three layers of stirring blades to ensure uniform temperature of the materials inside. A temperature probe is installed at the center of the reaction tank to monitor the temperature inside. The heat transfer oil, through the second PLC module 14, controls the power of the heating rods 15 in the hot oil tank, achieving a temperature of up to 200℃. When a high-temperature reaction is required in the reaction tank, the second solenoid valve 11 is opened, allowing the heated heat transfer oil to enter the reaction tank jacket and circulate, raising the temperature of the materials. After the high-temperature reaction is complete, when the material temperature is >100℃, the material is under high pressure inside the tank, posing a safety risk if discharged directly. To quickly cool and discharge the material, the second solenoid valve 11 is closed, and the first solenoid valve 6 is opened to allow cooling water to enter. This lowers the temperature of the heat transfer oil in the tank jacket 9, thereby cooling the materials inside the tank and ensuring safe discharge. This system design allows for the high-temperature reaction and rapid cooling of materials within the tank for safe discharge without the need for external steam, all through the tank jacket 9.
[0039] The above description is an explanation of the present utility model and not a limitation thereof. The scope of the present utility model is defined by the claims. Within the protection scope of the present utility model, any form of modification may be made.
Claims
1. A high-temperature oil bath reaction vessel system for proteins in a laboratory, characterized in that: The reaction vessel includes a tank jacket (9) and an insulation layer (17) sequentially arranged on the outer wall of the reaction vessel. A heating system and a cooling system are respectively arranged on the outer sides of the reaction vessel. The heating system inputs heat transfer oil from the bottom side of the tank jacket (9) and circulates heat transfer oil from the top side of the tank jacket (9); The cooling system discharges cooling coils from the bottom side of the tank jacket (9), and the cooling coils are arranged around the bottom of the tank jacket (9) to the top. The cooling system inputs cooling water through the cooling coils and circulates cooling water from the top.
2. The protein high temperature oil bath reaction vessel system for laboratory of claim 1, wherein: The reaction vessel is equipped with three layers of stirring blades, which are driven by a servo motor located at the top of the vessel. A temperature probe is also installed inside the reaction vessel to measure the temperature.
3. The protein high temperature oil bath reaction vessel system for laboratory of claim 1, wherein: The bottom left side of the tank jacket (9) is provided with a heat transfer oil inlet pipe (10), and the top right side is provided with a heat transfer oil outlet pipe (7); the bottom of the tank jacket (9) is provided with a cooling water inlet pipe (8), and the top left side is provided with a cooling water outlet pipe (16).
4. A protein high temperature oil bath reaction vessel system for use in a laboratory as claimed in claim 3, wherein: The heating system includes a hot oil tank, in which a heating rod (15) is installed. A heat transfer oil pump (12) and a second solenoid valve (11) are connected in sequence to the bottom discharge end of the hot oil tank. The liquid outlet end of the heat transfer oil pump (12) is connected to the heat transfer oil inlet pipe (10).
5. A protein high temperature oil bath reaction vessel system for use in a laboratory as claimed in claim 4, wherein: The path from the hot oil tank to the second solenoid valve (11) is also arranged with a second PLC module (14) and a second temperature sensor (13).
6. A protein high temperature oil bath reaction vessel system for use in a laboratory as defined in claim 3, wherein: The cooling system includes a cold water tank, and the internal circulation of the cold water tank is equipped with a chiller unit (1) and a unit circulation pump (2). The bottom outlet of the cold water tank is connected in sequence to a chiller pump (5) and a first solenoid valve (6), and the first solenoid valve (6) is connected to the cooling water inlet pipe (8).
7. A protein high temperature oil bath reaction vessel system for use in a laboratory as claimed in claim 6, wherein: The path from the chiller unit (1) to the chiller pump (5) is also connected in sequence to the first PLC module (3) and the first temperature sensor (4).