Biologics Sample Gradient Dilution Positioning Frame

Abstract

This utility model relates to the technical field of biomedical engineering and discloses a gradient dilution positioning rack for biological agents. It includes a chassis, a rotating shaft fixedly connected to the top center of the chassis, a protective mechanism at the top of the rotating shaft, and a limiting shell fixedly connected to the top of the chassis. A heating component is provided on the inner wall of the limiting shell. The protective mechanism includes a chuck, the bottom of which is fixedly connected to the top of the rotating shaft, and multiple fixing slots on the top of the chuck. In this utility model, the valve cap closes naturally to prevent liquid leakage; the anti-contamination plate isolates adjacent test tubes, reducing cross-contamination; and the protective cap, after rotating and closing, forms a closed space with the chuck, which, combined with the cleaning component, allows for further cleaning. This effectively prevents liquid leakage and aerosol diffusion, solving the problem of contamination prevention that existing technologies cannot achieve, and improving the safety and reliability of sample processing.

Description

Technical Field

[0001] This invention belongs to the technical field of biomedical engineering, and particularly relates to a positioning rack for gradient dilution of biological agents. Background Technology

[0002] A gradient dilution positioning frame for biological agents is a device used in biological experiments to precisely position and stably fix the container holding the sample when performing gradient dilution of biological agents. Through preset holes or slot structures, it provides a stable placement space for the sample container, ensuring that the experimenter or automated pipetting equipment can accurately add the sample and diluent to different containers, thereby achieving stepwise dilution of the sample concentration according to a certain ratio. It is an indispensable auxiliary tool in biological agent research and development, clinical testing, and microbial culture experiments, and directly affects the accuracy and reproducibility of experimental results.

[0003] Early serial dilution positioning frames for biological agents were mostly simple one-piece plastic or metal plates with fixed-size holes, only suitable for containers of specific sizes. This structure had significant drawbacks: firstly, the large gaps between the container and the holes caused container movement, affecting pipetting accuracy; secondly, the entire device was completely open, and aerosols, liquid splashes, and accidental spillage during pipetting could cause cross-contamination between different samples. To improve these issues, existing positioning frames typically use adjustable elastic slots to secure containers of different sizes and reduce container movement; some positioning frames also incorporate simple baffles or edges. While the frame does prevent direct liquid splashing to some extent, the existing device's anti-contamination capability remains poor. Its operation primarily relies on the clamping force of the elastic slots to secure the container and the baffles to prevent large liquid particles from splashing, thus avoiding contamination caused by significant container shaking and direct liquid splashing. However, because the pipetting area of ​​the existing device is still semi-open, the movement of the pipette tip into and out of the container during pipetting causes airflow, leading to the diffusion of aerosols generated by the sample within the device. Furthermore, the container tops lack sealing or isolation structures, with the open ends of adjacent containers directly facing each other. Aerosols and volatile sample components can then be transmitted through the air to other containers, making cross-contamination difficult to avoid. Utility Model Content

[0004] The purpose of this invention is to provide a gradient dilution positioning rack for biological agent samples, which aims to solve the technical problem in the prior art where the top of the container does not have a sealing or isolation structure, and the open ends of adjacent containers are directly opposite each other, making cross-contamination difficult to avoid.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a gradient dilution positioning frame for biological agents, comprising a chassis, a rotating shaft fixedly connected to the top center of the chassis, a protective mechanism provided at the top of the rotating shaft, a limiting shell fixedly connected to the top of the chassis, and a heating assembly provided on the inner wall of the limiting shell.

[0006] The protective mechanism includes a chuck, the bottom of which is fixedly connected to the top of the rotating shaft. The top of the chuck has multiple fixing slots, and test tubes are slidably connected to the inner walls of the multiple fixing slots. A limit ring is fixed to the top of the outer wall of the test tubes, and a valve cap is fixedly connected to the top of the test tubes. A dirt-proof plate is fixedly connected to the top of the test tubes. A protective cover is rotatably connected to the outer wall of the chuck, and a cleaning component is provided on the top of the protective cover.

[0007] As a further description of the above technical solution:

[0008] The heating assembly includes a preheating block, the outer wall of which is fixedly connected to the inner wall of the limiting shell, a monitor fixedly connected to the outer wall of the preheating block, a heating plate rotatably connected to the bottom of the preheating block, multiple heating holes opened at the top, and a corrugated plate fixedly connected to the inner wall of the preheating block.

[0009] As a further description of the above technical solution:

[0010] The cleaning assembly includes multiple disinfection lamps, the outer walls of which are respectively fixedly connected to the inner wall of the protective cover around the perimeter. A capacitor block is fixedly connected to the top of the protective cover, a protective shell is fixedly connected to the inner wall of the capacitor block, and a cover plate is slidably connected to the top of the capacitor block.

[0011] As a further description of the above technical solution:

[0012] A suction cup is fixedly connected to the bottom of the chassis, and a telescopic rod is fixedly connected to the left side of the outer wall of the chassis.

[0013] As a further description of the above technical solution:

[0014] The top of the telescopic rod is fixedly connected to a bracket, and the outer wall of the bracket is fixedly connected to multiple hooks.

[0015] As a further description of the above technical solution:

[0016] A drawer is slidably connected to the rear side of the bracket, and a handle is fixedly connected to the rear side of the drawer.

[0017] As a further description of the above technical solution:

[0018] The bottoms of the plurality of limiting rings are slidably connected to the top of the anti-fouling plate, and the outer walls of the plurality of valve covers are fixedly connected to the inner wall of the anti-fouling plate.

[0019] As a further description of the above technical solution:

[0020] The bottom of the outer wall of the rotating shaft is connected to the bottom of the inner wall of the preheating block, and the bottom of the wave plate is fixedly connected to the top of the heating plate.

[0021] This utility model has the following beneficial effects:

[0022] 1. In this utility model, the valve cover closes naturally to prevent liquid leakage; the anti-contamination plate can isolate adjacent test tubes and reduce cross-contamination; after the protective cover is rotated and closed, it forms a closed space with the chuck, which can be further cleaned in combination with the cleaning components, effectively preventing liquid leakage and aerosol diffusion, solving the problem that the existing technology cannot achieve anti-contamination, and improving the safety and reliability of sample processing.

[0023] 2. In this utility model, the monitor monitors the temperature in real time and, together with the heating plate and heating holes, accurately conducts heat to the sample to ensure a constant temperature. The corrugated plate enhances the heat preservation effect and reduces heat loss, thus achieving constant temperature protection for the sample during transportation. This effectively solves the problem that existing technologies cannot maintain cell activity during transportation and improves the stability and reliability of biological agent sample transportation. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 A perspective view provided for an embodiment of this utility model;

[0026] Figure 2 A front view provided for an embodiment of this utility model;

[0027] Figure 3 An exploded view of the protective mechanism provided in an embodiment of this utility model;

[0028] Figure 4 An exploded view of the cleaning components provided in an embodiment of this utility model;

[0029] Figure 5 This is an exploded view of the heating assembly provided in an embodiment of the present invention.

[0030] The following are the labeling elements in the figure:

[0031] 1—Chassis; 2—Protective Mechanism; 201—Chuck; 202—Fixing Groove; 203—Test Tube; 204—Limiting Ring; 205—Valve Cover; 206—Anti-fouling Plate; 207—Protective Cover; 208—Cleaning Component; 2081—Disinfection Lamp; 2082—Capacitor Block; 2083—Protective Shell; 2084—Cover Plate; 3—Heating Component; 301—Preheating Block; 302—Monitor; 303—Heating Plate; 304—Heating Hole; 305—Wave Plate; 4—Rotating Shaft; 5—Limiting Shell; 6—Suction Cup; 7—Telescopic Rod; 8—Bracket; 9—Hook; 10—Drawer Cabinet; 11—Handle. Detailed Implementation

[0032] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the embodiments of the present invention, and should not be construed as limiting the present invention.

[0033] In the description of the embodiments of this utility model, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0034] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0035] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0036] Reference Figure 1 , Figure 3 and Figure 4 This utility model provides an embodiment of a gradient dilution positioning rack for biological agents, comprising a base 1, which serves as the supporting foundation for the entire device and is used to stably place and fix other components; a rotating shaft 4 is fixedly connected to the top center of the base 1, which can drive a protective mechanism 2 to rotate, facilitating operation and observation of samples from different angles by experimental personnel; a protective mechanism 2 is provided at the top of the rotating shaft 4, which protects the sample from external contamination and provides space for sample placement and processing; a limiting shell 5 is fixedly connected to the top of the base 1, which limits the position of a heating component 3 and provides a certain degree of protection; a heating component 3 is provided on the inner wall of the limiting shell 5, which maintains a suitable temperature for the sample during transportation or storage to maintain cell viability.

[0037] The protective mechanism 2 includes a chuck 201, the bottom of which is fixedly connected to the top of the rotating shaft 4. The chuck 201 serves as the main structure of the protective mechanism 2, supporting and fixing the test tube 203. Multiple fixing grooves 202 are provided on the top of the chuck 201, and the test tube 203 is slidably connected to the inner wall of each groove. The fixing grooves 202 provide a stable placement position for the test tube 203, ensuring that it does not shake or tip over during operation. A limiting ring 204 is fixed to the top of the outer wall of the test tube 203, used to restrict the test tube. The position of test tube 203 in the fixing groove 202 prevents test tube 203 from being excessively inserted into or detached from the fixing groove 202; a valve cap 205 is fixedly connected to the top of test tube 203, which is in a closed state under natural conditions, effectively preventing liquid leakage and external contaminants from entering test tube 203; a contamination plate 206 is fixedly connected to the top of chuck 201, which is used to isolate adjacent test tubes 203 and reduce the risk of cross-contamination between samples; a protective cover 207 is rotatably connected to the outer wall of chuck 201, which can be rotated to secure the test tube 203. The chuck 201 is now opened and closed to protect the internal samples from external contamination when not in use. A cleaning component 208 is located on the top of the protective cover 207. This component cleans and disinfects the interior of the protective mechanism 2, maintaining a hygienic sample processing environment. The cleaning component 208 includes multiple disinfection lamps 2081, the outer walls of which are fixedly connected to the inner walls of the protective cover 207. These lamps emit ultraviolet light or other disinfecting rays to sterilize the interior of the protective mechanism 2. The top of the protective cover 207... A capacitor block 2082 is fixedly connected to the capacitor block 2081 to provide power and enable the electronic components of the disinfection lamp 2081 to work normally. A protective shell 2083 is fixedly connected to the inner wall of the capacitor block 2082 to protect the electronic components inside the capacitor block 2082 from external physical impact or liquid corrosion. A cover plate 2084 is slidably connected to the top of the capacitor block 2082 to cover the capacitor block 2082, providing further protection and facilitating the maintenance or battery replacement of the capacitor block 2082.

[0038] Specifically, the chassis 1 fixes the rotating shaft 4, providing support for the whole and fixing the limiting shell 5. The rotating shaft 4 drives the chuck 201 to rotate, facilitating the operation of the protective mechanism 2. The limiting shell 5 fixes the heating component 3, which maintains the sample activity. The chuck 201 slides to connect the test tube 203 through the fixing groove 202. The limiting ring 204 restricts the position of the test tube 203 to prevent it from being over-inserted or detached. The valve cover 205 closes to block liquid leakage and external contamination. The anti-contamination plate 206 isolates adjacent test tubes 203 to reduce cross-contamination. The protective cover 207 rotates to close the chuck 201, protecting the internal sample. In the cleaning component 208 on its top, the disinfection lamp 2081 sterilizes the inside. The capacitor block 2082 powers the disinfection lamp 2081. The protective shell 2083 protects the internal components of the capacitor block 2082. The cover plate 2084 covers the capacitor block 2082 and facilitates maintenance.

[0039] Reference Figure 1 , Figure 2 and Figure 5 The heating assembly 3 includes a preheating block 301, which serves as the main structure of the heating assembly 3. The preheating block 301 is used to install and fix other heating components and provides basic insulation. The outer wall of the preheating block 301 is fixedly connected to the inner wall of the limiting shell 5. The overall positioning of the heating assembly 3 is achieved through the fixed cooperation with the limiting shell 5. A monitor 302 is fixedly connected to the outer wall of the preheating block 301. The monitor 302 is used to monitor the temperature changes inside the heating assembly 3 and around the test tube 203 in real time, so as to adjust the heating state in a timely manner. A heating plate 303 is rotatably connected to the bottom of the heating block 301. The heating plate 303 can be rotated to adjust its relative position with the test tube 203 to ensure uniform heating. Multiple heating holes 304 are provided on the top of the heating plate 303. The heating holes 304 are used to concentrate the heat generated by the heating plate 303 to the bottom of the test tube 203 to improve heating efficiency. A corrugated plate 305 is fixedly connected to the inner wall of the preheating block 301. The corrugated plate 305 increases the heat reflection area through its corrugated structure, reduces heat loss, and enhances the heat preservation effect.

[0040] Specifically, in the heating assembly 3, the outer wall of the preheating block 301 is fixed to the inner wall of the limiting shell 5 to achieve overall positioning of the heating assembly 3. It serves as the main structure for installing and fixing other heating components and provides basic insulation. The monitor 302 fixed to the outer wall of the preheating block 301 monitors the temperature changes inside and around the test tube 203 in real time so as to adjust the heating state in time. The heating plate 303 rotatably connected to the bottom of the preheating block 301 can be rotated to adjust its relative position with the test tube 203 to ensure heating uniformity. Multiple heating holes 304 on the top of the heating plate 303 concentrate the heat generated by the heating plate 303 to the bottom of the test tube 203 to improve heating efficiency. The corrugated plate 305 fixed to the inner wall of the preheating block 301 increases the heat reflection area through its corrugated structure, reduces heat loss, and enhances the insulation effect.

[0041] Reference Figure 1 and Figure 2 A suction cup 6 is fixedly connected to the bottom of the chassis 1. The suction cup 6 can firmly fix the chassis 1 to the placement surface through adsorption, preventing the device from sliding during operation or transportation. A telescopic rod 7 is fixedly connected to the left side of the outer wall of the chassis 1. The telescopic rod 7 can be adjusted in length by telescoping to meet the needs of different heights. A bracket 8 is fixedly connected to the top of the telescopic rod 7. The bracket 8 is used to install and support hooks 9 and drawers 10, providing tool storage space. Multiple hooks 9 are fixedly connected to the outer wall of the bracket 8. The hooks 9 can be used to hang experimental tools such as pipettes and pipette tips for easy access and organization. A drawer 10 is slidably connected to the rear side of the bracket 8. The drawer 10 can be opened and closed by sliding and is used to store experimental consumables or record data. A handle 11 is fixedly connected to the rear side of the drawer 10, which makes it easy for the operator to pull the drawer. Cabinet 10 is designed to open and close. The bottoms of multiple limiting rings 204 are slidably connected to the top of the anti-fouling plate 206. This sliding connection not only provides auxiliary limiting for the test tubes 203 but also does not affect the placement and removal of the test tubes 203. The outer walls of multiple valve caps 205 are fixedly connected to the inner walls of the anti-fouling plate 206. The anti-fouling plate 206 is used to fix the valve caps 205, ensuring their stable position and better preventing leakage. The bottom of the outer wall of the rotating shaft 4 is connected to the bottom of the inner wall of the preheating block 301, so that the rotating shaft 4 and the preheating block 301 form a heat conduction path, which helps to transfer heat to the test tubes 203. The bottom of the corrugated plate 305 is fixedly connected to the top of the heating plate 303. This fixing method makes the corrugated plate 305 and the heating plate 303 tightly combined, enhancing heat reflection and heat preservation.

[0042] Specifically, the suction cup 6 at the bottom of the chassis 1 fixes the chassis 1 by adsorption, preventing the device from sliding. The telescopic rod 7 on the left side of the chassis 1 can be extended and adjusted to adapt to different heights. The bracket 8 at the top of the telescopic rod 7 is equipped with a load-bearing hook 9 and a drawer 10 to provide storage space. The hook 9 on the outer wall of the bracket 8 hangs experimental tools for easy access and organization. The drawer 10 on the back of the bracket 8 slides open and close to store consumables or documents. The handle 11 on the back of the drawer 10 makes it easy to pull the drawer 10. The bottom of multiple limiting rings 204 is slidably connected to the top of the anti-fouling plate 206 to assist in limiting the test tubes 203 without affecting the loading and unloading. The outer wall of multiple valve caps 205 is fixed to the inner wall of the anti-fouling plate 206. The anti-fouling plate 206 fixes the valve caps 205 to ensure their stability and prevent leakage. The bottom of the outer wall of the rotating shaft 4 is connected to the bottom of the inner wall of the preheating block 301, forming a heat conduction path to help the heat be transferred to the test tubes 203. The bottom of the corrugated plate 305 is fixed to the top of the heating plate 303, and the tight combination enhances the heat reflection and insulation effect.

[0043] Working principle: When liquid leaks from test tube 203, the valve cover 205 is in a closed state under normal conditions. Its cross-shaped cut can immediately block most of the overflowing liquid. The small amount of liquid that is not completely blocked drips onto the anti-fouling plate 206 under the action of gravity. The anti-fouling plate 206 prevents the liquid from spreading on the surface of the device and forming a large area of ​​contamination. The protective cover 207 is tightly fastened to the chuck 201 through a snap-fit ​​structure. When the protective cover 207 is closed, it triggers the sensing circuit of the capacitor block 2082, which causes the disinfection lamp 2081 to start and release ultraviolet light, thus making up for the shortcomings of the existing device that only relies on physical isolation and lacks active disinfection function.

[0044] Furthermore, when it is necessary to transport biological agent samples, the monitor 302 is activated to trigger the heating plate 303 to start. The heating plate 303 converts electrical energy into heat energy, and the heat is precisely conducted to the bottom of the test tube 203 through the heating hole 304, directly acting on the sample area. This ensures that the corrugated plate 305 reduces heat loss to the external environment. The preheating block 301 wraps around the outer wall of the test tube 203, which not only prevents the test tube 203 from falling out of its position due to transportation bumps, but also ensures that temperature fluctuations are controlled within the cell's tolerance range, effectively solving the problem that existing technologies cannot stably maintain cell activity during transportation.

[0045] The rest of this embodiment is the same as that in Embodiment 1. Features not explained in this embodiment are explained using the methods in Embodiment 1, and will not be repeated here.

[0046] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A biological agent sample gradient dilution positioning rack comprising a base tray (1) characterised in that: A rotating shaft (4) is fixedly connected to the top center of the chassis (1), and a protective mechanism (2) is provided at the top of the rotating shaft (4). A limiting shell (5) is fixedly connected to the top of the chassis (1), and a heating component (3) is provided on the inner wall of the limiting shell (5). The protective mechanism (2) includes a chuck (201), the bottom of which is fixedly connected to the top of the rotating shaft (4). The top of the chuck (201) has multiple fixing grooves (202), and test tubes (203) are slidably connected to the inner walls of the multiple fixing grooves (202). A limit ring (204) is fixed to the top of the outer wall of the test tubes (203). A valve cap (205) is fixedly connected to the top of the test tubes (203). A dirt-proof plate (206) is fixedly connected to the top of the chuck (201). A protective cover (207) is rotatably connected to the outer wall of the chuck (201). A cleaning component (208) is provided on the top of the protective cover (207).

2. The biological sample gradient dilution positioning rack of claim 1, wherein: The heating assembly (3) includes a preheating block (301), the outer wall of which is fixedly connected to the inner wall of the limiting shell (5), a monitor (302) is fixedly connected to the outer wall of the preheating block (301), a heating plate (303) is rotatably connected to the bottom of the preheating block (301), a plurality of heating holes (304) are opened on the top of the (303), and a corrugated plate (305) is fixedly connected to the inner wall of the preheating block (301).

3. The biological sample gradient dilution positioning rack of claim 1, wherein: The cleaning component (208) includes multiple disinfection lamps (2081), the outer walls of which are fixedly connected to the inner walls of the protective cover (207) around the perimeter. A capacitor block (2082) is fixedly connected to the top of the protective cover (207), a protective shell (2083) is fixedly connected to the inner wall of the capacitor block (2082), and a cover plate (2084) is slidably connected to the top of the capacitor block (2082).

4. The biological sample gradient dilution positioning rack of claim 1, wherein: A suction cup (6) is fixedly connected to the bottom of the chassis (1), and a telescopic rod (7) is fixedly connected to the left side of the outer wall of the chassis (1).

5. The biological agent sample gradient dilution positioning rack of claim 4, wherein: The top of the telescopic rod (7) is fixedly connected to a bracket (8), and the outer wall of the bracket (8) is fixedly connected to multiple hooks (9).

6. The biological agent sample gradient dilution positioning rack of claim 5, wherein: A drawer (10) is slidably connected to the rear side of the bracket (8), and a handle (11) is fixedly connected to the rear side of the drawer (10).

7. The biological sample gradient dilution positioning rack of claim 1, wherein: The bottoms of the plurality of limiting rings (204) are slidably connected to the top of the anti-fouling plate (206), and the outer walls of the plurality of valve covers (205) are fixedly connected to the inner wall of the anti-fouling plate (206).

8. The biological sample gradient dilution positioning rack of claim 2, wherein: The bottom of the outer wall of the rotating shaft (4) is connected to the bottom of the inner wall of the preheating block (301), and the bottom of the wave plate (305) is fixedly connected to the top of the heating plate (303).

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