Microbial and Cell Fluorescent Staining Detection Kit

By incorporating storage components and a fixing mechanism into the microbial and cell fluorescence staining detection kit, the problem of unstable locking mechanisms has been solved, ensuring the closure stability and safety of the kit during transportation and enhancing the user experience.

CN224448654UActive Publication Date: 2026-07-03YINGHE MEDICAL TECHNOLOGY (TIANJIN) CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
YINGHE MEDICAL TECHNOLOGY (TIANJIN) CO LTD
Filing Date
2025-08-20
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing microbial and cell fluorescence staining detection kits have unstable locking mechanisms when exposed to bumps or weightlessness, which may cause them to loosen and affect transportation safety.

Method used

The kit employs a storage component and fixing mechanism design, including a rectangular array layout of storage chambers for fungal fluorescent staining solution bottles, slides, sample preservation solution bottles, and anti-fluorescence quenching mirror oil bottles. Combined with the use of T-shaped strips and T-shaped grooves, and the linkage design of fixing frames, sliding blocks, springs, and insert rods, the kit ensures the closure stability of the kit.

Benefits of technology

The reagent kit is designed to be difficult to open during transportation and movement, ensuring closure stability and improving transportation safety. It also provides cushioning and shock absorption through silicone strips to prevent reagent leakage or slide breakage caused by drops or collisions, making it suitable for laboratory or field environments.

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Abstract

This utility model discloses a microbial and cell fluorescence staining detection kit, belonging to the technical field of reagent kits. It includes a box body with symmetrically arranged placement blocks inside. The box body also contains a storage component, and a fixing mechanism is located on the outer side of the box body. The storage component and the fixing mechanism work together. The storage component includes a storage cavity for fungal fluorescence staining solution bottles, which is located on one side of two placement blocks close to each other. T-shaped strips are fixedly connected to the inner wall of the box body in a rectangular array, and T-shaped grooves are formed in a rectangular array on the outer sides of the two placement blocks. This microbial and cell fluorescence staining detection kit, through its storage component and fixing mechanism, ensures correct use of the kit while preventing it from opening during transportation and movement, ensuring the kit's closure stability and transportation safety. It has a simple structure and strong practicality.
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Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, and in particular to a reagent kit for detecting microorganisms and cell fluorescence staining. Background Technology

[0002] The detection of fungi, bacteria, and cellular elements in human body fluid or skin samples primarily relies on microscopic observation. Based on sample processing methods, there are mainly two methods: conventional chemical staining microscopy and fluorescent special staining microscopy. Conventional chemical staining microscopy is simple and highly specific.

[0003] A search revealed a microbial and cellular fluorescence staining detection kit (authorization announcement number: CN 216350339U), which "includes an inner box, a glass slide, a sample processing and preservation solution bottle, a fungal fluorescence staining solution bottle, an anti-fluorescence quenching immersion oil bottle, a bacterial and cellular fluorescence staining solution bottle, lens paper, and a coverslip. The inner box has grooves for the glass slide, sample processing and preservation solution, fungal fluorescence staining solution, anti-fluorescence quenching immersion oil, bacterial and cellular fluorescence staining solution, lens paper, and coverslip. This utility model provides a comprehensive supply of reagents and materials for the entire process of microbial and cellular fluorescence staining detection of human body fluids or skin samples, including sample processing and preservation, sample smearing, fungal, bacterial, and cellular fluorescence staining, anti-fluorescence quenching, and objective lens maintenance. This combination greatly facilitates the operator, improves work efficiency, and standardizes the entire detection process."

[0004] Based on the aforementioned technologies, the applicant believes that although the aforementioned technologies can self-lock the box, the method of locking by relying on the weight of the box itself has certain defects. When staff carry the box by hand, if they encounter bumps or the box loses weight, it may loosen. The locking mechanism is not stable enough. In response to the above problems, we have launched a microbial and cell fluorescence staining detection kit. Utility Model Content

[0005] This utility model discloses a microbial and cell fluorescence staining detection kit, which aims to solve the technical problem that the kit may loosen or become unstable when encountering bumps or weightlessness.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A microbial and cell fluorescence staining detection kit includes a box body with symmetrically arranged placement blocks inside. The box body also contains a storage component, and a fixing mechanism is located on the outer side of the box body. The storage component and the fixing mechanism work together. The storage component includes a fungal fluorescence staining solution bottle storage cavity, which is located on one side of two placement blocks close to each other. Each of the two placement blocks has an anti-fluorescence quenching microlens bottle storage cavity on the side furthest from the fungal fluorescence staining solution bottle storage cavity. A slide storage cavity and a sample preservation solution bottle storage cavity are located on the side of the two placement blocks close to each other, between the anti-fluorescence quenching microlens bottle storage cavity and the fungal fluorescence staining solution bottle storage cavity. These three storage cavities work together. T-shaped strips are fixedly connected in a rectangular array on the inner wall of the box body. T-shaped grooves are formed in a rectangular array on the outer side of the two placement blocks, and the T-shaped strips and T-shaped grooves work together.

[0008] The designed storage components and fixing mechanism ensure the correct use of the reagent kit while preventing it from opening during transportation and movement. This guarantees the kit's closure stability, ensures safe transportation, and features a simple structure with strong practicality.

[0009] In a preferred embodiment, the fixing mechanism includes a fixing component and a protective component, which cooperate with each other. The fixing component includes a fixing frame, which is symmetrically fixedly connected to the top of the box. Sliding blocks are symmetrically slidably connected inside the two fixing frames. A spring is provided inside the two fixing frames and between the two sliding blocks. Insert rods are slidably connected to both sides of the inside of the two fixing frames. The insert rods are fixedly connected to the sliding blocks. A clearance groove is provided on the top of the two fixing frames. An adjusting block is fixedly connected to the top of the sliding block. The adjusting block is slidably connected to the clearance groove.

[0010] The fixing mechanism adopts a linkage design of fixing frame, sliding block, spring and plug rod, which makes the installation and disassembly of protective plate more convenient. The cooperation between adjustment block and clearance groove facilitates operation and improves experimental efficiency. The elastic reset function of spring ensures that plug rod automatically returns to its position, enhancing fixing stability.

[0011] In a preferred embodiment, the protective component includes positioning grooves symmetrically formed inside the housing. Slots are formed inside the housing and above the positioning grooves. Protective plates are inserted into both slots. Fixing holes are symmetrically formed on the top of both protective plates, and the fixing holes and insert rods cooperate with each other.

[0012] The protective plate is precisely positioned using slots and positioning grooves, and the locking mechanism of the fixing holes and insertion rods facilitates installation and disassembly.

[0013] In a preferred embodiment, the bottom of the box is symmetrically and fixedly connected with silicone strips for cushioning.

[0014] The silicone strip at the bottom of the box provides cushioning and shock absorption to prevent the reagent kit from leaking internal reagents or breaking glass slides due to drops or collisions, making it suitable for laboratory or field environments.

[0015] In a preferred embodiment, a handle is fixedly connected to the top of the box.

[0016] The handle design makes it easy to carry with one hand, especially suitable for scenarios where reagent kits need to be moved frequently, thus improving the user experience.

[0017] In a preferred embodiment, the size of the T-shaped strip and the size of the T-shaped groove are matched, and the T-shaped strip and the T-shaped groove are used in conjunction with each other.

[0018] The precise matching of the dimensions of the T-shaped strip and the T-shaped groove ensures a tight fit between the placement block and the box, preventing loosening due to tolerances, while also facilitating modular assembly and maintenance.

[0019] The microbial and cell fluorescence staining detection kit provided by this invention has the following advantages:

[0020] Firstly, the storage components and fixing mechanism ensure the correct use of the reagent kit, while also preventing the kit from opening during transportation and movement, thus ensuring the kit's own closure stability and transportation safety. The structure is simple and highly practical.

[0021] Secondly, the silicone strip at the bottom of the box provides cushioning and shock absorption to prevent the reagent kit from falling or colliding, which could cause internal reagent leakage or glass slide breakage. It is suitable for laboratory or field environments. The handle design makes it easy to carry with one hand, especially suitable for scenarios where reagent kits need to be moved frequently, thus improving the user experience. The T-shaped strip and T-shaped groove are precisely matched in size to ensure a tight fit between the placement block and the box, avoiding loosening due to tolerances, while also facilitating modular assembly and maintenance. Attached Figure Description

[0022] Figure 1 This is a three-dimensional schematic diagram of the microbial and cell fluorescence staining detection kit proposed in this utility model.

[0023] Figure 2 This is a three-dimensional bottom view of the microbial and cell fluorescence staining detection kit proposed in this utility model.

[0024] Figure 3This is a three-dimensional schematic diagram of the box of the microbial and cell fluorescence staining detection kit proposed in this utility model.

[0025] Figure 4 This is a three-dimensional schematic diagram of the placement block of the microbial and cell fluorescence staining detection kit proposed in this utility model.

[0026] Figure 5 This is a three-dimensional schematic diagram of the fixation component of the microbial and cell fluorescence staining detection kit proposed in this utility model.

[0027] In the attached diagram: 1. Box body; 2. Placement block; 3. Storage cavity for fungal fluorescent staining solution bottle; 4. Slide storage cavity; 5. Storage cavity for sample preservation solution bottle; 6. Storage cavity for anti-fluorescence quenching mirror oil bottle; 7. T-shaped strip; 8. T-shaped groove; 91. Fixing frame; 92. Sliding block; 93. Spring; 94. Clearance groove; 95. Adjustment block; 96. Insert rod; 101. Positioning groove; 102. Slot; 103. Protective plate; 104. Fixing hole; 11. Silicone strip; 12. Handle. Detailed Implementation

[0028] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and marked in the accompanying drawings can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0029] The microbial and cell fluorescence staining detection kit disclosed in this utility model is mainly used in kit application scenarios.

[0030] Reference Figure 1 - Figure 5A microbial and cell fluorescence staining detection kit includes a box 1, with symmetrically arranged placement blocks 2 inside the box 1. The box 1 also contains a storage component, and a fixing mechanism is located on the outside of the box 1. The storage component and the fixing mechanism work together. The storage component includes a fungal fluorescence staining solution bottle storage cavity 3, which is located on one side of the two placement blocks 2 that are close to each other. Both placement blocks 2 have anti-fluorescence quenching immersion oil bottle storage cavities 6 located on the side of the two placement blocks 2 that is furthest from the fungal fluorescence staining solution bottle storage cavity 3. On one side of the two placement blocks 2 that are close to each other and located between the anti-fluorescence quenching mirror oil bottle storage cavity 6 and the fungal fluorescent staining solution bottle storage cavity 3, there is a slide storage cavity 4 and a sample preservation solution bottle storage cavity 5. The fungal fluorescent staining solution bottle storage cavity 3, the slide storage cavity 4, the sample preservation solution bottle storage cavity 5 and the anti-fluorescence quenching mirror oil bottle storage cavity 6 are used together. The inner wall of the box body 1 is fixedly connected with T-shaped strips 7 in a rectangular array. The outer sides of the two placement blocks 2 are provided with T-shaped grooves 8 in a rectangular array. The T-shaped strips 7 and the T-shaped grooves 8 are used together. The fixing mechanism includes a fixing component and a protective component, which work together. The fixing component includes a fixing frame 91, which is symmetrically fixed to the top of the box 1. Sliding blocks 92 are symmetrically slidably connected inside each of the two fixing frames 91. A spring 93 is located inside each of the two fixing frames 91 and between the two sliding blocks 92. Insert rods 96 are slidably connected to both sides of the inside of each of the two fixing frames 91, and are fixedly connected to the sliding blocks 92. A clearance groove 94 is provided on the top of each of the two fixing frames 91. An adjusting block 95 is fixedly connected to the top of each sliding block 92, and is slidably connected to the clearance groove 94. The protective component includes a positioning groove 101, which is symmetrically located inside the box 1. A slot 102 is provided inside the box 1 and above the positioning groove 101. A protective plate 103 is inserted into each of the two slots 102. Fixing holes 104 are symmetrically provided on the top of each of the two protective plates 103, and the fixing holes 104 work together with the insert rods 96.

[0031] In this embodiment: After opening the protective plate 103, the reagent is taken out from the fungal fluorescent staining solution bottle storage chamber 3 and the sample preservation solution bottle storage chamber 5. The sample is prepared on the slide in the slide storage chamber 4. After adding the immersion oil from the anti-fluorescence quenching immersion oil bottle storage chamber 6, the sample is observed. When the reagent kit needs to be closed, the operator holds the two adjusting blocks 95 on the same side by hand. The two adjusting blocks 95 move towards each other, which drives the two insert rods 96 on the same side to move towards each other. Then, the two protective plates 103 are inserted into the slots 102 and the positioning slots 101. The insert rods 96 of the fixing mechanism automatically engage with the fixing holes 104 of the protective plate 103 under the action of the spring 93. Through the set storage components and fixing mechanism, the correct use of the reagent kit is achieved. At the same time, the reagent kit is prevented from opening during transportation and movement, ensuring the closure stability of the reagent kit itself, ensuring transportation safety, and the structure is simple and highly practical.

[0032] In the above technical solution, considering the possibility of loosening due to bumps or weightlessness of the box, and the instability of the locking mechanism, the specific operation is as follows to solve this problem:

[0033] Reference Figure 1 - Figure 5 In a preferred embodiment, silicone strips 11 for cushioning are symmetrically fixedly connected to the bottom of the box body 1. A handle 12 is fixedly connected to the top of the box body 1. The size of the T-shaped strip 7 matches the size of the T-shaped groove 8, and the T-shaped strip 7 and the T-shaped groove 8 are used in conjunction with each other.

[0034] In this embodiment: the silicone strip 11 at the bottom of the box 1 provides a cushioning and shock absorption function to prevent the reagent kit from falling or colliding, which could cause internal reagent leakage or glass slide breakage. It is suitable for laboratory or field environments. The handle 12 is designed for easy one-handed carrying, especially suitable for scenarios where reagent kits need to be moved frequently, thus improving the user experience. The T-shaped strip 7 and T-shaped groove 8 are precisely matched in size to ensure a tight fit between the mounting block 2 and the box 1, avoiding loosening due to tolerances, and facilitating modular assembly and maintenance.

[0035] Working principle: In use, the user carries the reagent kit through the handle 12, opens the protective plate 103, and takes out the reagent from the fungal fluorescent staining solution bottle storage chamber 3 and the sample preservation solution bottle storage chamber 5. The sample is prepared on the slide in the slide storage chamber 4, and the immersion oil in the anti-fluorescence quenching immersion oil bottle storage chamber 6 is added and observed. When it is necessary to close the reagent kit, the operator pinches the two adjusting blocks 95 on the same side. The two adjusting blocks 95 move towards each other, which drives the two insert rods 96 on the same side to move towards each other. Then, the two protective plates 103 are inserted into the slots 102 and the positioning grooves 101. The insert rods 96 of the fixing mechanism are automatically locked into the fixing holes 104 of the protective plate 103 under the action of the spring 93 to ensure safe transportation. The silicone strip 11 buffers vibration, and the cooperation between the T-shaped strip 7 and the T-shaped groove 8 maintains the stability of the internal structure, realizing efficient management and protection of the reagent.

[0036] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. The substitutions may be replacements of some structures, devices, or method steps, or they may be complete technical solutions. Equivalent substitutions or modifications made based on the technical solution and inventive concept of this utility model should all be covered within the protection scope of this utility model.

Claims

1. A kit for fluorescent staining of microorganisms and cells, comprising a box (1), characterized in that: The box (1) is symmetrically provided with placement blocks (2) inside, the box (1) is provided with storage components inside, and the box (1) is provided with fixing mechanisms on the outside. The storage components and fixing mechanisms are used in cooperation with each other. The storage assembly includes a fungal fluorescent staining solution bottle storage cavity (3), which is located on one side of two placement blocks (2) that are close to each other. An anti-fluorescence quenching mirror oil bottle storage cavity (6) is provided inside the two placement blocks (2) on the side away from the fungal fluorescent staining solution bottle storage cavity (3). A slide storage cavity (4) and a sample preservation solution bottle storage cavity (5) are provided on the side of the two placement blocks (2) that are close to each other and between the anti-fluorescence quenching mirror oil bottle storage cavity (6) and the fungal fluorescent staining solution bottle storage cavity (3). The fungal fluorescent staining solution bottle storage cavity (3), the slide storage cavity (4), the sample preservation solution bottle storage cavity (5), and the anti-fluorescence quenching mirror oil bottle storage cavity (6) are used in conjunction with each other. The inner wall of the box body (1) is fixedly connected with T-shaped strips (7) in a rectangular array. The outer side of the two placement blocks (2) is provided with T-shaped grooves (8) in a rectangular array. The T-shaped strips (7) and the T-shaped grooves (8) are used in conjunction with each other.

2. The microbial and cellular fluorescent staining test kit according to claim 1, characterized in that: The fixing mechanism includes a fixing component and a protective component, which are used in cooperation with each other. The fixing component includes a fixing frame (91), which is symmetrically fixedly connected to the top of the box (1). Sliding blocks (92) are symmetrically slidably connected inside the two fixing frames (91). A spring (93) is provided inside the two fixing frames (91) and between the two sliding blocks (92). Insert rods (96) are slidably connected to both sides inside the two fixing frames (91). The insert rods (96) are fixedly connected to the sliding blocks (92). A clearance groove (94) is provided on the top of the two fixing frames (91). An adjusting block (95) is fixedly connected to the top of the sliding block (92). The adjusting block (95) is slidably connected to the clearance groove (94).

3. The microbial and cellular fluorescent staining test kit according to claim 2, characterized in that: The protective component includes a positioning groove (101) which is symmetrically opened inside the box (1). A slot (102) is opened inside the box (1) and above the positioning groove (101). A protective plate (103) is inserted into the two slots (102). A fixing hole (104) is symmetrically opened on the top of the two protective plates (103). The fixing hole (104) and the insertion rod (96) are used in cooperation with each other.

4. The microbial and cellular fluorescent staining test kit according to claim 1, characterized in that: The bottom of the box (1) is symmetrically and fixedly connected with silicone strips (11) for cushioning.

5. The microbial and cellular fluorescent staining test kit according to claim 1, characterized in that: A handle (12) is fixedly connected to the top of the box (1).

6. The microbial and cellular fluorescent staining test kit according to claim 1, characterized in that: The size of the T-shaped strip (7) matches the size of the T-shaped groove (8), and the T-shaped strip (7) and the T-shaped groove (8) are used in conjunction with each other.