An ultrasonic cell disruptor

Abstract

This utility model discloses an ultrasonic cell disruptor, including a disruption chamber with an amplitude transformer on its upper side and a quick-fixing mechanism. The quick-fixing mechanism includes a cooling cup, rectangular mounting boxes, rubber rings, a rotating rod, and a rubber top. An electric telescopic rod is located at the bottom of the disruption chamber, with a holding plate fixedly connected to its telescopic end. A cooling cup is placed on the upper surface of the holding plate, and a beaker is placed inside the cooling cup. Rectangular mounting boxes are evenly distributed on the upper surface of the cooling cup. Rubber rings are fixedly connected to the side of each rectangular mounting box facing the center of the cooling cup and the side facing away from the center of the cooling cup. A rotating rod is movably fitted onto the inner arc surface of every two rubber rings located on the same side. This utility model can quickly fix and position beakers of different sizes for subsequent disruption operations.

Description

Technical Field

[0001] This utility model relates to the field of pharmacokinetic research technology, specifically to an ultrasonic cell disruptor. Background Technology

[0002] Ultrasonic cell disruptors are widely used in pharmacokinetic research. The working principle of an ultrasonic cell disruptor is to use the cavitation and mechanical effects of high-intensity ultrasound to generate strong impact and shear force on the cell membrane and cell wall, causing them to rupture and release intracellular contents. This is beneficial for the extraction and purification of intracellular biomolecules such as proteins, DNA, and RNA.

[0003] In the prior art, the patent with authorization publication number CN202323560616.8 discloses an ultrasonic cell disruptor, which has the following problems in actual use: for example, after the tissue sample is placed in the beaker, when the beaker is placed on the rotating platform, the beaker is not fixed, which can easily cause problems such as tipping over and inaccurate positioning, making subsequent disruption operations inconvenient. Utility Model Content

[0004] The technical problem to be solved by this invention is to overcome the existing defects and provide an ultrasonic cell disruptor that can quickly fix and position beakers of different sizes for subsequent disruption operations, thus effectively solving the problems in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an ultrasonic cell disruptor, comprising a disruption chamber, an amplitude transformer on the upper side of the disruption chamber, and a quick-fixing mechanism;

[0006] The quick-fixing mechanism includes a cooling cup, a rectangular mounting box, rubber rings, a rotating rod, and a rubber top. The bottom of the crushing box is equipped with an electric telescopic rod, and the telescopic end of the electric telescopic rod is fixedly connected to a holding plate. The cooling cup is placed on the upper surface of the holding plate, and a holding beaker is placed inside the cooling cup. The upper surface of the cooling cup is equipped with evenly distributed rectangular mounting boxes. Rubber rings are fixedly connected to the side of the rectangular mounting box facing the center of the cooling cup and the side facing away from the center of the cooling cup, respectively. A rotating rod is movably fitted on the inner arc surface of every two rubber rings located on the same side. A rubber top is fixedly connected to the end of the three rotating rods facing the center of the cooling cup, respectively.

[0007] The system also includes a controller, which is located on the upper side of the crushing box. The input end of the controller is electrically connected to an external power source, and the input end of the electric telescopic rod is electrically connected to the output end of the controller.

[0008] Furthermore, the quick-fixing mechanism also includes a fixing plate, external threads, and limiting sleeves. The fixing plates are respectively fixedly connected to the inner center of the three rectangular mounting boxes. External threads are respectively provided on the middle part of the outer arc surface of the three rotating rods. The external threads are connected to the middle thread of the fixing plate located in the same rectangular mounting box. Limiting sleeves are respectively provided on the front and rear sides of the outer arc surface of the rotating rods. The external threads are respectively located between the two limiting sleeves on the same side, realizing the function of clamping the beaker on three sides.

[0009] Furthermore, the quick-fixing mechanism also includes a toggle head, which is fixedly connected to the end of each of the three rotating rods away from the center of the cooling cup, providing power for fixing the beaker.

[0010] Furthermore, glass covers are fixedly connected to the upper side of the rectangular mounting box, and scale lines are provided on the upper side of the glass covers to enable the function of visual observation and positioning.

[0011] Furthermore, the upper surface of the cooling cup is provided with evenly distributed placement grooves, and ice baskets are placed inside the cooling cup to achieve the cooling function.

[0012] Furthermore, the inner wall of the cooling cup is provided with evenly distributed rectangular flow holes, and the front side of the cooling cup is provided with a water leakage hole to realize the function of cold water flow.

[0013] Furthermore, an ultrasonic generator is placed on the right side of the crushing box. A transducer is fixedly connected to the left end of the output wire of the ultrasonic generator. The output shaft of the transducer is fixedly connected to the upper end of the amplitude transformer to provide power for the operation of the amplitude transformer.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] The beaker is held in place by three relatively close rubber tips, which can quickly fix the beaker when animal tissue fluid is poured into beakers of different sizes. This allows for rapid fixation of the beaker, and the position of the beaker can then be controlled up and down to allow the animal tissue fluid inside the beaker to contact the amplitude transformer. The amplitude transformer then breaks down the cells inside the animal tissue. This method can quickly fix and position beakers of different sizes for subsequent breaking operations. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0017] Figure 2 This is a cross-sectional structural diagram of the crushing box of this utility model;

[0018] Figure 3 This is an exploded structural diagram of the rapid fixing mechanism of this utility model;

[0019] Figure 4This is an enlarged structural diagram of point A of this utility model.

[0020] In the diagram: 1. Crushing box, 2. Electric telescopic rod, 3. Quick fixing mechanism, 31. Cooling cup, 32. Rectangular mounting box, 33. Fixing plate, 34. Rubber ring, 35. Rotating rod, 36. External thread, 37. Limiting sleeve, 38. Twist head, 39. Rubber top, 4. Placement slot, 5. Ice basket, 6. Drain hole, 7. Glass cover, 8. Ultrasonic generator, 9. Transducer, 10. Amplitude rod, 11. Controller, 12. Beaker. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-4 This embodiment provides a technical solution: an ultrasonic cell disruptor, including a disruption chamber 1, an amplitude transformer 10 on the upper side of the disruption chamber 1, an ultrasonic generator 8 placed on the right side of the disruption chamber 1, a transducer 9 fixedly connected to the left end of the output wire of the ultrasonic generator 8, and the output shaft of the transducer 9 fixedly connected to the upper end of the amplitude transformer 10; it also includes a controller 11, which is disposed on the upper side of the disruption chamber 1, and the input end of the controller 11 is electrically connected to an external power supply.

[0023] The system also includes a quick-fixing mechanism 3; the quick-fixing mechanism 3 includes a cooling cup 31, a rectangular mounting box 32, rubber rings 34, a rotating rod 35, and a rubber top 39. The bottom of the crushing box 1 is equipped with an electric telescopic rod 2, the input end of which is electrically connected to the output end of a controller 11. A holding plate is fixedly connected to the telescopic end of the electric telescopic rod 2. A cooling cup 31 is placed on the upper surface of the holding plate, and a holding beaker 12 is placed inside the cooling cup 31. Evenly distributed rectangular mounting boxes 32 are provided on the upper surface of the cooling cup 31. Rubber rings 34 are fixedly connected to the side of the rectangular mounting box 32 facing the center of the cooling cup 31 and the side facing away from the center of the cooling cup 31, respectively. Each pair of rubber rings 34 located on the same side has a movable inner arc surface fitted with... A rotating rod 35 and three rotating rods 35 are respectively fixedly connected to rubber heads 39 at their ends facing the center of the cooling cup 31; the quick fixing mechanism 3 also includes a fixing plate 33, an external thread 36 and a limiting sleeve 37. The fixing plate 33 is respectively fixedly connected to the middle of the interior of three rectangular mounting boxes 32. The outer arc surface of the three rotating rods 35 is respectively provided with an external thread 36. The external thread 36 is threadedly connected to the middle of the fixing plate 33 located in the same rectangular mounting box 32. The front and rear sides of the outer arc surface of the rotating rod 35 are respectively provided with a limiting sleeve 37. The external thread 36 is located between two limiting sleeves 37 on the same side; the quick fixing mechanism 3 also includes a twisting head 38. The twisting head 38 is respectively fixedly connected to the end of the three rotating rods 35 away from the center of the cooling cup 31.

[0024] Among them, a glass cover 7 is fixedly connected to the upper side of the rectangular mounting box 32, and a scale line is provided on the upper side of the glass cover 7. The upper surface of the cooling cup 31 is provided with evenly distributed placement grooves 4. An ice basket 5 is placed inside the cooling cup 31. The inner side wall of the cooling cup 31 is provided with evenly distributed rectangular flow holes. A water leakage hole 6 is provided on the front side of the cooling cup 31.

[0025] The working principle of this utility model is as follows:

[0026] When the ultrasonic cell disruptor is needed, the cooling cup 31 can be picked up manually, and the liquid containing animal tissue to be disrupted can be poured into the holding beaker 12. Then the holding beaker 12 is placed inside the cooling cup 31.

[0027] At this point, the head 38 can be turned, which in turn turns the rod 35. Due to the threaded connection between the external thread 36 and the middle of the fixed plate 33, the rod 35 will move through the two rubber rings 34 when it is turned. The rod 35 can be moved to the designated position according to the markings on the scale. At this point, the three rubber caps 39 press against the outer surface of the beaker 12. Then, the rubber stopper is inserted into the drain hole 6, and the ice cubes are placed into the ice basket 5. The ice basket 5 is then placed into the placement slot 4. A certain amount of water is added to the cooling cup 31. The water is cooled by the ice cubes and wraps around the outer surface of the beaker 12. The cooling cup 31 is then placed on the upper surface of the placement plate.

[0028] At this time, the controller 11 can be adjusted to operate the electric telescopic rod 2. The DC motor inside the electric telescopic rod 2 is powered on, and the output shaft of the motor is rigidly connected to a precision lead screw through a coupling. When the motor rotates, it drives the lead screw to rotate synchronously. A matching nut is fitted on the lead screw, and the nut is fixedly connected to one end of the telescopic rod. The nut is restricted from rotating. When the lead screw rotates, the nut moves linearly back and forth along the axis of the lead screw under the action of the thread thrust of the lead screw, thereby driving the telescopic rod to extend or retract, which in turn pushes the holding plate to move up and down, thereby causing the cooling cup 31 to move up and down until the liquid in the holding beaker 12 is immersed in the outer arc surface of the amplitude transformer 10, and the moving up and down of the holding plate stops.

[0029] At this point, the operation of the ultrasonic generator 8 can be controlled. The ultrasonic generator 8 converts the current into a high-frequency electrical signal. At this time, the transducer converts the high-frequency electrical signal into mechanical vibration. The amplitude transformer amplifies the vibration amplitude of the transducer and transmits the vibration directly to the liquid sample, triggering cavitation effect and mechanical impact, ultimately achieving cell disruption.

[0030] After completing the task, remove the cooling cup 31, release the fixing to the holding beaker 12, then remove the holding beaker 12, then remove the ice basket 5, and pull out the rubber stopper. Excess water can drain out through the drain hole 6.

[0031] It is worth noting that the core chip of the controller 11 disclosed in the above embodiments is a single-chip microcomputer, specifically the S7-200. The electric telescopic rod 2, ultrasonic generator 8, transducer 9, and amplitude transformer 10 can be freely configured according to the actual application scenario. It is recommended that the electric telescopic rod 2 be an LX835 model electric servo push rod, the ultrasonic generator 8 be a TS-UGC model ultrasonic generator, the transducer 9 be a YP-6015-4BZ series ultrasonic transducer, and the amplitude transformer 10 can be an ultrasonic amplitude transformer of appropriate specifications according to the working requirements. The amplitude transformer 10 controls the operation of the electric telescopic rod 2 using methods commonly used in the prior art.

[0032] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. An ultrasonic cell disruptor, comprising a disruption chamber (1), wherein an amplitude transformer (10) is provided on the upper side of the disruption chamber (1), characterized in that: Including a quick-fixing mechanism (3); The quick-fixing mechanism (3) includes a cooling cup (31), a rectangular mounting box (32), a rubber ring (34), a rotating rod (35), and a rubber top (39). The bottom of the crushing box (1) is provided with an electric telescopic rod (2). The telescopic end of the electric telescopic rod (2) is fixedly connected to a holding plate. The cooling cup (31) is placed on the upper surface of the holding plate. A holding beaker (12) is placed inside the cooling cup (31). The upper surface of the cooling cup (31) is provided with evenly distributed rectangular mounting boxes (32). The side of the rectangular mounting box (32) facing the center of the cooling cup (31) and the side away from the center of the cooling cup (31) are respectively fixedly connected with rubber rings (34). A rotating rod (35) is movably fitted on the inner arc surface of every two rubber rings (34) located on the same side. A rubber top (39) is fixedly connected to one end of the three rotating rods (35) facing the center of the cooling cup (31). Among them, there is also a controller (11), which is located on the upper side of the crushing box (1). The input end of the controller (11) is electrically connected to an external power source, and the input end of the electric telescopic rod (2) is electrically connected to the output end of the controller (11).

2. An ultrasonic cell disruptor according to claim 1, wherein: The quick-fixing mechanism (3) also includes a fixing plate (33), an external thread (36), and a limiting sleeve (37). The fixing plate (33) is fixedly connected to the middle of the interior of the three rectangular mounting boxes (32). The outer arc surface of the three rotating rods (35) is provided with external threads (36) respectively. The external threads (36) are threaded to the middle of the fixing plate (33) located in the same rectangular mounting box (32). The front and rear sides of the outer arc surface of the rotating rod (35) are provided with limiting sleeves (37) respectively. The external threads (36) are located between the two limiting sleeves (37) on the same side.

3. An ultrasonic cell disruptor according to claim 2, wherein: The quick-fixing mechanism (3) also includes a toggle head (38), which is fixedly connected to one end of the three rotating rods (35) away from the center of the cooling cup (31).

4. An ultrasonic cell disruptor according to claim 1, wherein: The upper side of the rectangular mounting box (32) is fixedly connected with a glass cover (7), and the upper side of the glass cover (7) is provided with scale lines.

5. An ultrasonic cell disruptor according to claim 1, wherein: The upper surface of the cooling cup (31) is provided with evenly distributed placement grooves (4), and ice baskets (5) are placed inside the cooling cup (31).

6. An ultrasonic cell disruptor according to claim 1, wherein: The inner wall of the cooling cup (31) is provided with evenly distributed rectangular flow holes, and the front side of the cooling cup (31) is provided with a water leakage hole (6).

7. An ultrasonic cell disruptor according to claim 1, wherein: An ultrasonic generator (8) is placed on the right side of the crushing box (1). A transducer (9) is fixedly connected to the left end of the output wire of the ultrasonic generator (8). The output shaft of the transducer (9) is fixedly connected to the upper end of the amplitude transformer (10).

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